Tan0003143 (gene) Snake gourd v1

Overview


Name	Tan0003143
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Auxin-responsive GH3 family protein
Location	LG05: 6046809 .. 6049283 (+)
RNA-Seq Expression	Tan0003143
Synteny	Tan0003143

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATGATAACACCATTGGTCTTGTTTTGTGGCCACTAAGCTTTCGTACATTTAATTCGGGTAGTGTAACTTTTTGCAGTAGTAGAAGTTTTTTTTTCTTTTGTCCAAATCAGTCCCTCTTCTCAGTCTTTTGCTTGCCCTTGCATAACAAAACACACACACCTAGCTAGCTTCTACCTGATTCTTATATATATAGATATAGGCCAAAAAAAAAAAAAAAAGAAAAAGGGTTATTATATGGGGTGTTTATAATGGAATTGGAAGAGTTGAGTGCGGCGGAGGAAGATGAAGAGATGATTGATTGGTTCGAGAAAGTGTCGAGAGAGGCAGGATTGGCTCAATCCCGCACTCTCCGTCAGATTCTCCAACAGAATTCTGGTGTGGAGTACCTGAAACAATGGCTCCGAAATGATGAATATGTCAATATCCATGAGATTCCAGCTGATGACTTTACATTCGAATCTCTCTTCACTTCCTTAGTCCCCCTCTCTTCTCACCCCGATTTCGAGCCTTATCTTCAACGTATTGCAGATGGCGACTCCACTCCTTTACTTACTCAACAACCCATTAAAACTCTCTCTTTAAGGTACCCGGCCTCTTTTTCTCTTTTTTTAATTCATTCATTTCATGCACTTCAACCATAAAAATAGAAGGATAAATGTCACTGTTGTTTTTTCCCGCTCTTTTGCTTCTTACTAATTTATATCCCGCTGCAGTTCTGGCACCACGGACGGCAGACAAAAGTACGTACCTTTTACTCCCCATAGTGCACAGACTACTCTTCTTATCTTTCGTTTAGCTGCTGCTTACAGATCCAGGTATTAATCATATACATAATAATAATATATTATTATTATTATTATTTATTCGTTTTCCTGATTTGATTTGATCTGATCTGATCACAATCAAATTCGTAATTGGAATAATGGGTAAAATTAGGGTTTATCCCACAAGGGAAGGAGGGAAAATTCTAGAGTTCATTTACAGCAGCAAACAGACGAAAACCAAAGGAGGAATAACAACCGGAACCGCCACAACCCATTACTACGCGAGTGATGAATTCAAAATCAAGCAGCTGAAAACCAAATCATTCACTTGCAGCCCACAGGAAGTGATTTCCGGCGGCGACTACAAGCAATCCACCTATTGCCATCTCCTTCTCGGCCTCTTCTTTAGCGACCAAATCGAGTTCGTCACCTCCACTTTTGCCTACAGCATAGTCCAAGCCTTTAACGAATTGGAAGACACTTGGCCCCAAATCTGCGACGACATTACCCACGCCACACTCTGCTCCAAAATCACCGTACCCAAAATCCGCAAGGCGGTTTTGAAGGTCTTGAGCACTCCGAACCCGACTCTGGGTTCCAGAATCCAATCCCTCTGCCGAGAGCTTCAGAGTGAGGACTGGGTGGGTTTGATTCCGAAGCTGTGGCCGAATTGCAAGTATGTTTATTCCATAATGACCGGGTCCATGCAGCCGTATGTGAAGAAATTGAGACACTACGCCGGTGGTTTGCCGTTGGTGAGTGCGGATTATGGATCCACAGAGAGTTGGATTGGGGTTAATCTGGATCCTGATTTACCGCCTGAAAATGTCACTTTTGCGGTGATTCCCACTTTCTCTTATTTCGAATTCATCCCACTTTTCAGATCACATGATCAACAAAATCATCATAGTGACTTCTTGGAAGGCCAACCCGTACCCTTGTCTAAAGTCAAGATTGGGCAAGAGTATGAAATCGTTCTTACAACTTTCACAGGTATAATTAATTTCTCTCATCTTCAATCTTTATAAGTATGATGTGATCAATATTGTGGAGATACTTAACATCCATCGGCCAGCTGTAATTGGGGTTGGAATATATAATATGGCAGGACTTTACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCTGGGTTTCACAACAATACGCCGAAACTAAACTTCATATGCAGAAGAAAGCTTGTATTAACAATAAACATAGACAAGAACACAGAGAAGGATCTCCAATTGGTGGTTGAAAGAGGGAGGCAGGTATTGGGGGAGGAAAGTGGAGGCGAGCTAGTTGATTTCACCAGCCACGCTGAGGTGGCGACGGAGCAACCTGGTCACTACGTCATATTCTGGGAAGTCAAAGGTGATGTGGACGACACAGTTTTCAGAAGATGTTGCTCAGAAATGGACGCTGCTTTCGTTGACTATGGCTACGTTGTCTCTCGAAGGTCCAATTCCATTGGACCTCTTGAGCTTCGGATTGTTCAGAGAGGCACTTTCAACAGTATTTTGGAACATTATATTCGAAATGGAGCTGCACTTAGCCAATTCAAGACCCCAAGATGTACTACAAATCACCTTCTTCTTACCATTCTCAATCTCTCTACTCTCAAAACCTTTCGCAGCTCTGCATACTCCTGACACTTTTTTTTCCTACAAAAACTTAAATGGAATCATCTAAAATCCAACCAACTTCAAAG

mRNA sequence

AGATGATAACACCATTGGTCTTGTTTTGTGGCCACTAAGCTTTCGTACATTTAATTCGGGTAGTGTAACTTTTTGCAGTAGTAGAAGTTTTTTTTTCTTTTGTCCAAATCAGTCCCTCTTCTCAGTCTTTTGCTTGCCCTTGCATAACAAAACACACACACCTAGCTAGCTTCTACCTGATTCTTATATATATAGATATAGGCCAAAAAAAAAAAAAAAAGAAAAAGGGTTATTATATGGGGTGTTTATAATGGAATTGGAAGAGTTGAGTGCGGCGGAGGAAGATGAAGAGATGATTGATTGGTTCGAGAAAGTGTCGAGAGAGGCAGGATTGGCTCAATCCCGCACTCTCCGTCAGATTCTCCAACAGAATTCTGGTGTGGAGTACCTGAAACAATGGCTCCGAAATGATGAATATGTCAATATCCATGAGATTCCAGCTGATGACTTTACATTCGAATCTCTCTTCACTTCCTTAGTCCCCCTCTCTTCTCACCCCGATTTCGAGCCTTATCTTCAACGTATTGCAGATGGCGACTCCACTCCTTTACTTACTCAACAACCCATTAAAACTCTCTCTTTAAGTTCTGGCACCACGGACGGCAGACAAAAGTACGTACCTTTTACTCCCCATAGTGCACAGACTACTCTTCTTATCTTTCGTTTAGCTGCTGCTTACAGATCCAGGGTTTATCCCACAAGGGAAGGAGGGAAAATTCTAGAGTTCATTTACAGCAGCAAACAGACGAAAACCAAAGGAGGAATAACAACCGGAACCGCCACAACCCATTACTACGCGAGTGATGAATTCAAAATCAAGCAGCTGAAAACCAAATCATTCACTTGCAGCCCACAGGAAGTGATTTCCGGCGGCGACTACAAGCAATCCACCTATTGCCATCTCCTTCTCGGCCTCTTCTTTAGCGACCAAATCGAGTTCGTCACCTCCACTTTTGCCTACAGCATAGTCCAAGCCTTTAACGAATTGGAAGACACTTGGCCCCAAATCTGCGACGACATTACCCACGCCACACTCTGCTCCAAAATCACCGTACCCAAAATCCGCAAGGCGGTTTTGAAGGTCTTGAGCACTCCGAACCCGACTCTGGGTTCCAGAATCCAATCCCTCTGCCGAGAGCTTCAGAGTGAGGACTGGGTGGGTTTGATTCCGAAGCTGTGGCCGAATTGCAAGTATGTTTATTCCATAATGACCGGGTCCATGCAGCCGTATGTGAAGAAATTGAGACACTACGCCGGTGGTTTGCCGTTGGTGAGTGCGGATTATGGATCCACAGAGAGTTGGATTGGGGTTAATCTGGATCCTGATTTACCGCCTGAAAATGTCACTTTTGCGGTGATTCCCACTTTCTCTTATTTCGAATTCATCCCACTTTTCAGATCACATGATCAACAAAATCATCATAGTGACTTCTTGGAAGGCCAACCCGTACCCTTGTCTAAAGTCAAGATTGGGCAAGAGTATGAAATCGTTCTTACAACTTTCACAGGACTTTACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCTGGGTTTCACAACAATACGCCGAAACTAAACTTCATATGCAGAAGAAAGCTTGTATTAACAATAAACATAGACAAGAACACAGAGAAGGATCTCCAATTGGTGGTTGAAAGAGGGAGGCAGGTATTGGGGGAGGAAAGTGGAGGCGAGCTAGTTGATTTCACCAGCCACGCTGAGGTGGCGACGGAGCAACCTGGTCACTACGTCATATTCTGGGAAGTCAAAGGTGATGTGGACGACACAGTTTTCAGAAGATGTTGCTCAGAAATGGACGCTGCTTTCGTTGACTATGGCTACGTTGTCTCTCGAAGGTCCAATTCCATTGGACCTCTTGAGCTTCGGATTGTTCAGAGAGGCACTTTCAACAGTATTTTGGAACATTATATTCGAAATGGAGCTGCACTTAGCCAATTCAAGACCCCAAGATGTACTACAAATCACCTTCTTCTTACCATTCTCAATCTCTCTACTCTCAAAACCTTTCGCAGCTCTGCATACTCCTGACACTTTTTTTTCCTACAAAAACTTAAATGGAATCATCTAAAATCCAACCAACTTCAAAG

Coding sequence (CDS)

ATGGAATTGGAAGAGTTGAGTGCGGCGGAGGAAGATGAAGAGATGATTGATTGGTTCGAGAAAGTGTCGAGAGAGGCAGGATTGGCTCAATCCCGCACTCTCCGTCAGATTCTCCAACAGAATTCTGGTGTGGAGTACCTGAAACAATGGCTCCGAAATGATGAATATGTCAATATCCATGAGATTCCAGCTGATGACTTTACATTCGAATCTCTCTTCACTTCCTTAGTCCCCCTCTCTTCTCACCCCGATTTCGAGCCTTATCTTCAACGTATTGCAGATGGCGACTCCACTCCTTTACTTACTCAACAACCCATTAAAACTCTCTCTTTAAGTTCTGGCACCACGGACGGCAGACAAAAGTACGTACCTTTTACTCCCCATAGTGCACAGACTACTCTTCTTATCTTTCGTTTAGCTGCTGCTTACAGATCCAGGGTTTATCCCACAAGGGAAGGAGGGAAAATTCTAGAGTTCATTTACAGCAGCAAACAGACGAAAACCAAAGGAGGAATAACAACCGGAACCGCCACAACCCATTACTACGCGAGTGATGAATTCAAAATCAAGCAGCTGAAAACCAAATCATTCACTTGCAGCCCACAGGAAGTGATTTCCGGCGGCGACTACAAGCAATCCACCTATTGCCATCTCCTTCTCGGCCTCTTCTTTAGCGACCAAATCGAGTTCGTCACCTCCACTTTTGCCTACAGCATAGTCCAAGCCTTTAACGAATTGGAAGACACTTGGCCCCAAATCTGCGACGACATTACCCACGCCACACTCTGCTCCAAAATCACCGTACCCAAAATCCGCAAGGCGGTTTTGAAGGTCTTGAGCACTCCGAACCCGACTCTGGGTTCCAGAATCCAATCCCTCTGCCGAGAGCTTCAGAGTGAGGACTGGGTGGGTTTGATTCCGAAGCTGTGGCCGAATTGCAAGTATGTTTATTCCATAATGACCGGGTCCATGCAGCCGTATGTGAAGAAATTGAGACACTACGCCGGTGGTTTGCCGTTGGTGAGTGCGGATTATGGATCCACAGAGAGTTGGATTGGGGTTAATCTGGATCCTGATTTACCGCCTGAAAATGTCACTTTTGCGGTGATTCCCACTTTCTCTTATTTCGAATTCATCCCACTTTTCAGATCACATGATCAACAAAATCATCATAGTGACTTCTTGGAAGGCCAACCCGTACCCTTGTCTAAAGTCAAGATTGGGCAAGAGTATGAAATCGTTCTTACAACTTTCACAGGACTTTACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCTGGGTTTCACAACAATACGCCGAAACTAAACTTCATATGCAGAAGAAAGCTTGTATTAACAATAAACATAGACAAGAACACAGAGAAGGATCTCCAATTGGTGGTTGAAAGAGGGAGGCAGGTATTGGGGGAGGAAAGTGGAGGCGAGCTAGTTGATTTCACCAGCCACGCTGAGGTGGCGACGGAGCAACCTGGTCACTACGTCATATTCTGGGAAGTCAAAGGTGATGTGGACGACACAGTTTTCAGAAGATGTTGCTCAGAAATGGACGCTGCTTTCGTTGACTATGGCTACGTTGTCTCTCGAAGGTCCAATTCCATTGGACCTCTTGAGCTTCGGATTGTTCAGAGAGGCACTTTCAACAGTATTTTGGAACATTATATTCGAAATGGAGCTGCACTTAGCCAATTCAAGACCCCAAGATGTACTACAAATCACCTTCTTCTTACCATTCTCAATCTCTCTACTCTCAAAACCTTTCGCAGCTCTGCATACTCCTGA

Protein sequence

MELEELSAAEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPTFSYFEFIPLFRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAYS

Homology

[Close]

BLAST of Tan0003143 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 3.0e-230
Identity = 397/599 (66.28%), Postives = 476/599 (79.47%), Query Frame = 0


Query: 3   LEELSAAEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEI 62
           +E + A  +D  +I WFE VS  A   QS TLR+IL+ NSGVEYL++WL     V++ ++
Sbjct: 1   METVEAGHDD--VIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGT---VDVEKM 60

Query: 63  PADDFTFESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKY 122
             DD+T E+LFTSLVP+ SH D +PY+QRIADG+++PLLTQ+PI  LSLSSGTT+GRQKY
Sbjct: 61  --DDYTLETLFTSLVPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKY 120

Query: 123 VPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYY 182
           VPFT HSAQTTL IFRL+AAYRSR YP REGG+ILEFIY+ K+ KT GG+T GTATTHYY
Sbjct: 121 VPFTRHSAQTTLQIFRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYY 180

Query: 183 ASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQA 242
           AS+EFK KQ  TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S Q+EFV S F+Y+IVQA
Sbjct: 181 ASEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQA 240

Query: 243 FNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSE-D 302
           F+  E+ W +IC DI    L S+IT+PK+RKAVL  L  PNP+L S I+ +C EL++   
Sbjct: 241 FSFFEEIWREICADIKEGNLSSRITLPKMRKAVL-ALIRPNPSLASHIEEICLELETNLG 300

Query: 303 WVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLP 362
           W GLI KLWPN K++ SIMTGSM PY+ KLRHYAGGLPLVSADYGSTESWIGVN+DP LP
Sbjct: 301 WFGLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLP 360

Query: 363 PENVTFAVIPTFSYFEFIPLFRSHDQQN--HHSDFLEGQPVPLSKVKIGQEYEIVLTTFT 422
           PE+V+FAVIPTFSYFEFIPL+R  +Q +     DF+E +PVPLS+VK+GQEYE+VLTTFT
Sbjct: 361 PEDVSFAVIPTFSYFEFIPLYRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFT 420

Query: 423 GLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEES 482
           GLYR RLGDVVEV  FH  TPKL+FI RRKL+LTINIDKNTEKDLQ VV++  Q+L   +
Sbjct: 421 GLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRST 480

Query: 483 GGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNS 542
             E+VDFTSHA+V   +PGHYVI+WE++G+ DD     CC EMD AFVDYGYVVSRR NS
Sbjct: 481 RAEVVDFTSHADVIA-RPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNS 540

Query: 543 IGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           IGPLELR+V+RGTF  + E  +     L+QFKTPRCTTN ++L ILN ST+K FRSSAY
Sbjct: 541 IGPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

[Close]

BLAST of Tan0003143 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 5.4e-171
Identity = 302/590 (51.19%), Postives = 415/590 (70.34%), Query Frame = 0


Query: 11  EDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFE 70
           + EE+I+ FE ++++AG  Q  TL++IL++N G EYL+QW  N +            T  
Sbjct: 12  DPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGK------------TDS 71

Query: 71  SLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSA 130
             F + +P+ +H D EPY+ RIADGD +P+LT +PI T+SLSSGTT G+ K+VPF     
Sbjct: 72  LSFKNCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELM 131

Query: 131 QTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIK 190
           ++T+ IF+ +  +R+R +P    GK L+FIY SKQ KTKGG+  GTATT+ Y + +FK  
Sbjct: 132 ESTMQIFKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKT 191

Query: 191 QLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTW 250
               ++  CSP EVI G D++QS YCHLL GL F D+++ V+STFA+SIV AF   E  W
Sbjct: 192 MKAMQTPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIW 251

Query: 251 PQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLW 310
            ++  +I    L S++ VP +R A+ K+L  P+P L   I + C  L   +W GLIP+L+
Sbjct: 252 QELVTNIREGVLSSRVIVPSMRAAMSKLLK-PDPELADTIFNKCSRL--SNWYGLIPELF 311

Query: 311 PNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVI 370
           PN +Y+Y IMTGSM+PY+KKLRHYAG LPL+SADYGS+E WIG N++P+LPPE VT+AV+
Sbjct: 312 PNTRYIYGIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVL 371

Query: 371 PTFSYFEFIPLFRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVV 430
           P   YFEFIPL         + D LE  PV L++VK+G+EYEIV+T F GLYR RLGDVV
Sbjct: 372 PNIGYFEFIPLM-------ENLDGLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVV 431

Query: 431 EVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHA 490
           ++ GFHN TP+L FICRR L+L+INIDKNTEKDLQL VE   ++L +E   E+VDFTSH 
Sbjct: 432 KIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEK-LEVVDFTSHV 491

Query: 491 EVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQR 550
            V+ + PGHYVIFWE+ G+  + + + CC+ +D +FVD GYV SR+ ++IG LELRIV+R
Sbjct: 492 NVSAD-PGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKR 551

Query: 551 GTFNSILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRSSAY 599
           GTF+ IL+H++  GAA+SQFKTPRC   TN  +L IL+ + ++++ S+A+
Sbjct: 552 GTFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

[Close]

BLAST of Tan0003143 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 596.3 bits (1536), Expect = 3.9e-169
Identity = 308/603 (51.08%), Postives = 413/603 (68.49%), Query Frame = 0


Query: 1   MELEELSAAEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIH 60
           M +E++    + E++I+ FE ++++AG  Q  TL++IL+QN G EYL+ W  N       
Sbjct: 3   MLVEKIEKKFDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLWGLNGR----- 62

Query: 61  EIPADDFTFESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQ 120
                  T    F + VP+ +H D EPY+QRIADGD +P+LT +PI+T+SLSSGTT G+ 
Sbjct: 63  -------TDPQTFKNCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKP 122

Query: 121 KYVPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH 180
           K+VPF     ++T+ IF+ + A+R+R +P    GK L+FIYSSKQ KTKGG+  GTATT+
Sbjct: 123 KFVPFNDELMESTMQIFKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTN 182

Query: 181 YYASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIV 240
            Y + +FK       +  CSP EVI G D+ QS YCHLL GL F D+++ V+STFA+SIV
Sbjct: 183 VYRNAQFKKTMKAMCTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIV 242

Query: 241 QAFNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSE 300
            AF   E  W  +  DI    L S++TVP IR A+ K+L  P+P L   I + C  L   
Sbjct: 243 HAFRTFEQVWEALVVDIREGVLSSRVTVPSIRLAMSKLLK-PDPELADTIYNKCSRL--S 302

Query: 301 DWVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDL 360
           +W GLIP L+PN +Y+Y IMTGSM+PY+KKLRHYAG LPL+SADYGS+E W+GVN++P L
Sbjct: 303 NWYGLIPDLFPNTRYIYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKL 362

Query: 361 PPENVTFAVIPTFSYFEFIPL---FRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTT 420
           PPE VT+AV+P   YFEFIPL       +Q N         PV L++VK+G+EYE+V T 
Sbjct: 363 PPELVTYAVLPNIGYFEFIPLGGNLNGIEQAN--------SPVGLTEVKLGEEYEVVFTN 422

Query: 421 FTGLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGE 480
           F GLYR RLGDVV+V GFHN TP+L F+CR  L+L+INIDKNTEKDLQL VE   + L +
Sbjct: 423 FAGLYRYRLGDVVKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVD 482

Query: 481 ESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRS 540
           E   E+VDFTSH  V+ + PGHYVIFWE+ G+  D + + CC+ +D +F+D GYV SR+ 
Sbjct: 483 EK-LEVVDFTSHVNVSAD-PGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKV 542

Query: 541 NSIGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRS 599
           N+IG LELRIV+RGTF+ IL+H++  G A+SQFKTPRC    N  LL IL+ + ++T+ S
Sbjct: 543 NAIGALELRIVKRGTFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVS 579

[Close]

BLAST of Tan0003143 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 7.1e-163
Identity = 294/591 (49.75%), Postives = 395/591 (66.84%), Query Frame = 0


Query: 13  EEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFESL 72
           EE I+ FE ++R+A   Q  TL++IL+ N+  EYL+ +                 T    
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGR------------TDAES 66

Query: 73  FTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQT 132
           + S +PL  H D EPY+QRI DGD++P++T +PI  LSLSSGTT G+ K++PF     +T
Sbjct: 67  YKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLET 126

Query: 133 TLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQL 192
           TL I+R + A+R+R YP  + GK L+F+Y SKQ  TKGGI   TATT+ Y    +K    
Sbjct: 127 TLQIYRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMK 186

Query: 193 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQ 252
             +S  CSP EVI G D+ QS YCHLL GL +S+++  V STFA+S+V AF   E+ W  
Sbjct: 187 DIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWED 246

Query: 253 ICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPN 312
           +C DI    L  K+T P IR+AV K+L  PNP L   I   C  L   +W G+IP LWPN
Sbjct: 247 LCTDIRDGVLSKKVTAPSIREAVSKILK-PNPELADSIYKKCIGL--SNWYGVIPALWPN 306

Query: 313 CKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPT 372
            KYVY IMTGSM+PY+KKLRHYAG LPL+SADYG++E W+G N+DP +PPE VT+AV+P 
Sbjct: 307 AKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQ 366

Query: 373 FSYFEFIPLFRSHDQQNHHS---DFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDV 432
             YFEFIPL +   ++  +S    ++E  PV L++V++G+ YE+V+T F GLYR RLGDV
Sbjct: 367 VGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDV 426

Query: 433 VEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSH 492
           V++A FHN+TP+L FICRR LVL+INIDKNTEKDLQL VE   + L E    E++DFTS 
Sbjct: 427 VKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFL-EGEKLEVMDFTSF 486

Query: 493 AEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQ 552
            E +++ PG YVIFWE+ GD  D V   C + +D AF+D GY  SR+  +IGPLELRI++
Sbjct: 487 VERSSD-PGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILR 546

Query: 553 RGTFNSILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRSSAY 599
           +GTF  IL+H++  G A+SQFKTPR    +N  +L IL+ +  +++ S+AY
Sbjct: 547 KGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

[Close]

BLAST of Tan0003143 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 553.1 bits (1424), Expect = 3.8e-156
Identity = 281/586 (47.95%), Postives = 389/586 (66.38%), Query Frame = 0


Query: 15  MIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFESLFT 74
           +ID F++++R A   Q +TL++IL +N    YL+    N    +           E  F 
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDP----------EEAFK 72

Query: 75  SLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQTTL 134
           S+VPL +  + EPY++R+ DGD++P+LT  P+  +SLSSGT+ GR K++PFT    + TL
Sbjct: 73  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 132

Query: 135 LIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQLKT 194
            +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 133 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 192

Query: 195 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQIC 254
            S +CSP EVI   D  Q+ YCHLL G+ F DQ+++V + FA+ +V AF   E  W +I 
Sbjct: 193 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 252

Query: 255 DDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPNCK 314
            DI    L ++ITVP +R A+ K+L TPNP L   I++ C  L   +W GLIP L+PN K
Sbjct: 253 TDIKDGVLSNRITVPSVRTAMSKLL-TPNPELAETIRTKCMSL--SNWYGLIPALFPNAK 312

Query: 315 YVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPTFS 374
           YVY IMTGSM+PYV KLRHYAG LPLVS DYGS+E WI  N+ P L PE  TFAVIP   
Sbjct: 313 YVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLG 372

Query: 375 YFEFIPLFRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVVEVAG 434
           YFEF+P+  + +         E +PV L++VKIG+EYE+V+T + GLYR RLGDVV+V G
Sbjct: 373 YFEFLPVSETGEG--------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIG 432

Query: 435 FHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHAEVAT 494
           F+NNTP+L FICRR L+L+INIDKNTE+DLQL VE   + L EE   E++DF+S+ +V+T
Sbjct: 433 FYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVST 492

Query: 495 EQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQRGTFN 554
           + PGHY IFWE+ G+ ++ V + CC+ +D AF+D GYV SR+  +IG LELR+V +GTF 
Sbjct: 493 D-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFR 552

Query: 555 SILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRSSAY 599
            I EH++  G++  QFK PRC   +N  +L IL  + + ++ S+A+
Sbjct: 553 KIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of Tan0003143 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1009.2 bits (2608), Expect = 1.5e-290
Identity = 492/596 (82.55%), Postives = 546/596 (91.61%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           EE E+M+DWFE+VS EAG+AQSRTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSLVPLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKI 189
           AQTTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYAS++FKI
Sbjct: 123 AQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKI 182

Query: 190 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDT 249
           KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTSTFAY+IVQAFNELED 
Sbjct: 183 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDC 242

Query: 250 WPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKL 309
           WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++IQ++C EL+ +DW+ L+PKL
Sbjct: 243 WPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIQAVCEELERQDWLDLVPKL 302

Query: 310 WPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAV 369
           W NCKYVYSIMTGSMQPY+KKLR YAGGLPLVS DYGSTESWIG+N+DP LPPENVTFAV
Sbjct: 303 WSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAV 362

Query: 370 IPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKIGQEYEIVLTTFTGLY 429
           +PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKIGQ+YE+VLTTFTGLY
Sbjct: 363 VPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLY 422

Query: 430 RCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGE 489
           RCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLVVERG Q+L ++SG E
Sbjct: 423 RCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAE 482

Query: 490 LVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGP 549
           LVDFTSHA++ T QPGHYVIFWEVKG+VDD V  RCCSEMDAAFVDYGYVVSRRSN+IGP
Sbjct: 483 LVDFTSHADL-TNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGP 542

Query: 550 LELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           LELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNLST+K F S+AY
Sbjct: 543 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Tan0003143 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1000.0 bits (2584), Expect = 8.9e-288
Identity = 488/596 (81.88%), Postives = 542/596 (90.94%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           EE E+M+DWFE+VS EAG+AQ RTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSL+PLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKI 189
           AQTTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYAS++FKI
Sbjct: 123 AQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKI 182

Query: 190 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDT 249
           KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTSTFAY+IVQAFNELED 
Sbjct: 183 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDC 242

Query: 250 WPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKL 309
           WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++I ++C EL+ +DW+ L+PKL
Sbjct: 243 WPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIGAVCEELERQDWLDLVPKL 302

Query: 310 WPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAV 369
           WPNCKYVYSIMTGSMQPY+KKLR YAG LPLVS DYGSTESWIGVN+DP LPPENVTFAV
Sbjct: 303 WPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAV 362

Query: 370 IPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKIGQEYEIVLTTFTGLY 429
           +PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKIGQ+YE+VLTTFTGLY
Sbjct: 363 VPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLY 422

Query: 430 RCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGE 489
           RCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLVVERG Q+L ++SG E
Sbjct: 423 RCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAE 482

Query: 490 LVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGP 549
           LVDFTSHA++ T QPGHYVIFWEVKG V+D V   CCSEMDAAFVDYGYVVSRRSN+IGP
Sbjct: 483 LVDFTSHADL-TNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGP 542

Query: 550 LELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           LELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNLST+K F S+AY
Sbjct: 543 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Tan0003143 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 999.2 bits (2582), Expect = 1.5e-287
Identity = 487/596 (81.71%), Postives = 542/596 (90.94%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           E+ E+M+DWFE+VS EAG+AQ RTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSL+PLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKI 189
           AQTTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYAS++FKI
Sbjct: 123 AQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKI 182

Query: 190 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDT 249
           KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTSTFAY+IVQAFNELED 
Sbjct: 183 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDC 242

Query: 250 WPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKL 309
           WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++I ++C EL+ +DW+ L+PKL
Sbjct: 243 WPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIGAVCEELERQDWLDLVPKL 302

Query: 310 WPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAV 369
           WPNCKYVYSIMTGSMQPY+KKLR YAG LPLVS DYGSTESWIGVN+DP LPPENVTFAV
Sbjct: 303 WPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAV 362

Query: 370 IPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKIGQEYEIVLTTFTGLY 429
           +PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKIGQ+YE+VLTTFTGLY
Sbjct: 363 VPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLY 422

Query: 430 RCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGE 489
           RCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLVVERG Q+L ++SG E
Sbjct: 423 RCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAE 482

Query: 490 LVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGP 549
           LVDFTSHA++ T QPGHYVIFWEVKG V+D V   CCSEMDAAFVDYGYVVSRRSN+IGP
Sbjct: 483 LVDFTSHADL-TNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGP 542

Query: 550 LELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           LELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNLST+K F S+AY
Sbjct: 543 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Tan0003143 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 998.8 bits (2581), Expect = 2.0e-287
Identity = 488/596 (81.88%), Postives = 542/596 (90.94%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           EE E+M+DWFE+VS EAG+AQ RTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSLVPLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKI 189
           AQTTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYAS++FKI
Sbjct: 123 AQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKI 182

Query: 190 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDT 249
           KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTSTFAY+IVQAFNELED 
Sbjct: 183 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDC 242

Query: 250 WPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKL 309
           WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++I ++C EL+ +DW+ L+PKL
Sbjct: 243 WPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIGAVCEELERQDWLDLVPKL 302

Query: 310 WPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAV 369
           WPNCKYVYSIMTGS+QPY+KKLR YAG LPLVS DYGSTESWIGVN+DP LPPENVTFAV
Sbjct: 303 WPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAV 362

Query: 370 IPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKIGQEYEIVLTTFTGLY 429
           +PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKIGQ+YE+VLTTFTGLY
Sbjct: 363 VPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLY 422

Query: 430 RCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGE 489
           RCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLVVERG Q+L ++SG E
Sbjct: 423 RCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAE 482

Query: 490 LVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGP 549
           LVDFTSHA++ T QPGHYVIFWEVKG V+D V   CCSEMDAAFVDYGYVVSRRSN+IGP
Sbjct: 483 LVDFTSHADL-TNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGP 542

Query: 550 LELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           LELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNLST+K F S+AY
Sbjct: 543 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Tan0003143 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 992.6 bits (2565), Expect = 1.4e-285
Identity = 489/611 (80.03%), Postives = 543/611 (88.87%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           EE E+M+DWFE+VS EAG+AQ RTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSL+PLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRS---------------RVYPTREGGKILEFIYSSKQTKTKGGITT 189
           AQTTLLIFR+AAAYRS               RVYPTREGG+ILEFIYSSKQTKTKGGITT
Sbjct: 123 AQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITT 182

Query: 190 GTATTHYYASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTST 249
           GTATTHYYAS++FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTST
Sbjct: 183 GTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTST 242

Query: 250 FAYSIVQAFNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLC 309
           FAY+IVQAFNELED WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++IQ++C
Sbjct: 243 FAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIQAVC 302

Query: 310 RELQSEDWVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGV 369
            EL+ +DW+ L+PKLWPNCKYVYSIMTGSMQPY+KKLR YAG LPLVS DYGSTESWIGV
Sbjct: 303 EELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGV 362

Query: 370 NLDPDLPPENVTFAVIPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKI 429
           N+DP LPPENVTFAV+PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKI
Sbjct: 363 NVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKI 422

Query: 430 GQEYEIVLTTFTGLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLV 489
           GQ+YE+VLTTFTGLYRCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLV
Sbjct: 423 GQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLV 482

Query: 490 VERGRQVLGEESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFV 549
           VERG Q+L ++SG ELVDFTSHA++ T QPGHYVIFWEVKG V+D V   CCSEMDAAFV
Sbjct: 483 VERGSQLLADQSGAELVDFTSHADL-TNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFV 542

Query: 550 DYGYVVSRRSNSIGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNL 599
           DYGYVVSRRSN+IGPLELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNL
Sbjct: 543 DYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNL 602

[Close]

BLAST of Tan0003143 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 7.1e-291
Identity = 492/596 (82.55%), Postives = 546/596 (91.61%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           EE E+M+DWFE+VS EAG+AQSRTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSLVPLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKI 189
           AQTTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYAS++FKI
Sbjct: 123 AQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKI 182

Query: 190 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDT 249
           KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTSTFAY+IVQAFNELED 
Sbjct: 183 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDC 242

Query: 250 WPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKL 309
           WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++IQ++C EL+ +DW+ L+PKL
Sbjct: 243 WPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIQAVCEELERQDWLDLVPKL 302

Query: 310 WPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAV 369
           W NCKYVYSIMTGSMQPY+KKLR YAGGLPLVS DYGSTESWIG+N+DP LPPENVTFAV
Sbjct: 303 WSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAV 362

Query: 370 IPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKIGQEYEIVLTTFTGLY 429
           +PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKIGQ+YE+VLTTFTGLY
Sbjct: 363 VPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLY 422

Query: 430 RCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGE 489
           RCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLVVERG Q+L ++SG E
Sbjct: 423 RCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAE 482

Query: 490 LVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGP 549
           LVDFTSHA++ T QPGHYVIFWEVKG+VDD V  RCCSEMDAAFVDYGYVVSRRSN+IGP
Sbjct: 483 LVDFTSHADL-TNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGP 542

Query: 550 LELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           LELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNLST+K F S+AY
Sbjct: 543 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Tan0003143 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 7.4e-288
Identity = 487/596 (81.71%), Postives = 542/596 (90.94%), Query Frame = 0


Query: 10  EEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTF 69
           E+ E+M+DWFE+VS EAG+AQ RTLR+IL+QN GVEYL QWL +   VNIH+IP DDF  
Sbjct: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGD---VNIHQIP-DDFAL 62

Query: 70  ESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHS 129
           ESLFTSL+PLSSH DFEP+LQRIADGDS+PLLTQQPI+TLSLSSGTT+GRQKYVPFTPHS
Sbjct: 63  ESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHS 122

Query: 130 AQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKI 189
           AQTTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYAS++FKI
Sbjct: 123 AQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKI 182

Query: 190 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDT 249
           KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQ+EFVTSTFAY+IVQAFNELED 
Sbjct: 183 KQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDC 242

Query: 250 WPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKL 309
           WPQIC DITHATLCS+IT+P+IRKAVL ++S PNP LG++I ++C EL+ +DW+ L+PKL
Sbjct: 243 WPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIGAVCEELERQDWLDLVPKL 302

Query: 310 WPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAV 369
           WPNCKYVYSIMTGSMQPY+KKLR YAG LPLVS DYGSTESWIGVN+DP LPPENVTFAV
Sbjct: 303 WPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAV 362

Query: 370 IPTFSYFEFIPLFRSHDQQNHHS-------DFLEGQPVPLSKVKIGQEYEIVLTTFTGLY 429
           +PTFSYFEFIPLFR H Q +  S       DFLEGQP+PLS+VKIGQ+YE+VLTTFTGLY
Sbjct: 363 VPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLY 422

Query: 430 RCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGE 489
           RCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKDLQLVVERG Q+L ++SG E
Sbjct: 423 RCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAE 482

Query: 490 LVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGP 549
           LVDFTSHA++ T QPGHYVIFWEVKG V+D V   CCSEMDAAFVDYGYVVSRRSN+IGP
Sbjct: 483 LVDFTSHADL-TNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGP 542

Query: 550 LELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           LELRIV+RG+FN I+EHYI NGAALSQFKTPRCTTN +LLTILNLST+K F S+AY
Sbjct: 543 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Tan0003143 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 5.3e-278
Identity = 479/605 (79.17%), Postives = 543/605 (89.75%), Query Frame = 0


Query: 1   MELEELSAAEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIH 60
           M+++EL   EE+E++IDWFE+VS EA +AQSRTLR+ILQQN GVEYLK+W+ +   VNIH
Sbjct: 1   MKIDEL--CEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGD---VNIH 60

Query: 61  EIPADDFTFESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQ 120
           EIP DDFT +S+FTSL+PLSSH  FEP+LQRIADGDS+PLLTQQPI TLSLSSGTT+GRQ
Sbjct: 61  EIP-DDFTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQ 120

Query: 121 KYVPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH 180
           KYVPFTPHSA TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH
Sbjct: 121 KYVPFTPHSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH 180

Query: 181 YYASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIV 240
           YYAS+EFKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S+++EFVTSTFAY+IV
Sbjct: 181 YYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIV 240

Query: 241 QAFNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSE 300
           QAFN+LE++W +I  DI+HATL S+I +P+IRKAVLKV+S P P LG +I+ +C EL+ E
Sbjct: 241 QAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMS-PKPELGWKIRRVCEELKKE 300

Query: 301 DWVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDL 360
            W+GLIPKLWPNCKYVYSIMTGSMQPY+KKLRHY GGLPLVS DYGSTESWIGVN+DP L
Sbjct: 301 GWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYL 360

Query: 361 PPENVTFAVIPTFSYFEFIPLFRSH------DQQNHHSDFLEGQPVPLSKVKIGQEYEIV 420
           PPENVTFAVIPTFSYFEFIPLF+ H      D   H  DFLEGQP+ LS+VKIGQ+YE+V
Sbjct: 361 PPENVTFAVIPTFSYFEFIPLFKQHATTSSTDTAIH--DFLEGQPIALSEVKIGQQYELV 420

Query: 421 LTTFTGLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQV 480
           LTTFTGLYRCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKD+QL VERG Q+
Sbjct: 421 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQL 480

Query: 481 LGEESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVS 540
           + + SG ELVDFTS+AE+ + QPGHYVIFWEVKGDV+DTV  +CCSEMDAAFVDYGYVVS
Sbjct: 481 MSQLSGAELVDFTSYAEL-SNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVS 540

Query: 541 RRSNSIGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFR 600
           R++NSIGPLELRIV+RG+FN ILE+YI NGAALSQFKTPRCTTNH LL ILNLSTL++F 
Sbjct: 541 RKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFF 595

[Close]

BLAST of Tan0003143 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 5.3e-278
Identity = 479/605 (79.17%), Postives = 543/605 (89.75%), Query Frame = 0


Query: 1   MELEELSAAEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIH 60
           M+++EL   EE+E++IDWFE+VS EA +AQSRTLR+ILQQN GVEYLK+W+ +   VNIH
Sbjct: 1   MKIDEL--CEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGD---VNIH 60

Query: 61  EIPADDFTFESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQ 120
           EIP DDFT +S+FTSL+PLSSH  FEP+LQRIADGDS+PLLTQQPI TLSLSSGTT+GRQ
Sbjct: 61  EIP-DDFTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQ 120

Query: 121 KYVPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH 180
           KYVPFTPHSA TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH
Sbjct: 121 KYVPFTPHSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTH 180

Query: 181 YYASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIV 240
           YYAS+EFKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S+++EFVTSTFAY+IV
Sbjct: 181 YYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIV 240

Query: 241 QAFNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSE 300
           QAFN+LE++W +I  DI+HATL S+I +P+IRKAVLKV+S P P LG +I+ +C EL+ E
Sbjct: 241 QAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMS-PKPELGWKIRRVCEELKKE 300

Query: 301 DWVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDL 360
            W+GLIPKLWPNCKYVYSIMTGSMQPY+KKLRHY GGLPLVS DYGSTESWIGVN+DP L
Sbjct: 301 GWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYL 360

Query: 361 PPENVTFAVIPTFSYFEFIPLFRSH------DQQNHHSDFLEGQPVPLSKVKIGQEYEIV 420
           PPENVTFAVIPTFSYFEFIPLF+ H      D   H  DFLEGQP+ LS+VKIGQ+YE+V
Sbjct: 361 PPENVTFAVIPTFSYFEFIPLFKQHATTSSTDTAIH--DFLEGQPIALSEVKIGQQYELV 420

Query: 421 LTTFTGLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQV 480
           LTTFTGLYRCRLGDVVEVAGFHN TPKLNFICRRKLVLT+NIDKNTEKD+QL VERG Q+
Sbjct: 421 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQL 480

Query: 481 LGEESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVS 540
           + + SG ELVDFTS+AE+ + QPGHYVIFWEVKGDV+DTV  +CCSEMDAAFVDYGYVVS
Sbjct: 481 MSQLSGAELVDFTSYAEL-SNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVS 540

Query: 541 RRSNSIGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFR 600
           R++NSIGPLELRIV+RG+FN ILE+YI NGAALSQFKTPRCTTNH LL ILNLSTL++F 
Sbjct: 541 RKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFF 595

[Close]

BLAST of Tan0003143 vs. ExPASy TrEMBL
Match: A0A6J1CHT7 (indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=LOC111011043 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 2.0e-277
Identity = 473/602 (78.57%), Postives = 535/602 (88.87%), Query Frame = 0


Query: 9   AEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFT 68
           +E+++E+IDWFE+V+ +AG+AQSRTLRQILQQN GVEYLK WL +   VNIH+I  DD  
Sbjct: 18  SEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGD---VNIHQIQDDDSA 77

Query: 69  FESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPH 128
            ESLFTS VPLSSHP FEP+LQRIADGDSTPLLTQ PIKTLSLSSGTT+GRQKYV FTPH
Sbjct: 78  LESLFTSSVPLSSHPHFEPFLQRIADGDSTPLLTQLPIKTLSLSSGTTEGRQKYVLFTPH 137

Query: 129 SAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFK 188
           SAQTTLLIFRLAAAYRSRVYP REGGKI+EFIYSSKQTKTKGGITTGTATTHYYAS+EFK
Sbjct: 138 SAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFK 197

Query: 189 IKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELED 248
           IKQLKT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ Q+EFVTSTFAY+I+QAFN+LE+
Sbjct: 198 IKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEE 257

Query: 249 TWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPK 308
            WP IC DITHA+L S +T+PK+R+AVL ++S PNP+L S I++ C+ L++ DW GLIP+
Sbjct: 258 VWPDICRDITHASLSSTVTIPKMRRAVLDIIS-PNPSLASSIRASCQRLRARDWFGLIPE 317

Query: 309 LWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFA 368
           LWPN KYVYSI TGSMQPYVKKLRHYA GLPLVSADYGSTESWIGVN+DP LPPENVTFA
Sbjct: 318 LWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFA 377

Query: 369 VIPTFSYFEFIPLFRSHDQQNH---------HSDFLEGQPVPLSKVKIGQEYEIVLTTFT 428
           V+PTFSYFEFIPLFR H  QN            DFLEG+PVPLS+VKIGQ+YE+VLTTFT
Sbjct: 378 VVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFT 437

Query: 429 GLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGE-- 488
           GLYRCRLGD+VEV+GFHN TPKL+FICRRKLVLTINIDKNTEKDLQ+VVERG Q+LG+  
Sbjct: 438 GLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKS 497

Query: 489 ESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRS 548
           +SG E+VDFTSHA+VA +QPGHYVIFWEVKGDV+D V   CC+ MDAAFVD+GYVVSRRS
Sbjct: 498 QSGAEVVDFTSHADVA-DQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDHGYVVSRRS 557

Query: 549 NSIGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSA 600
           NSIGPLELR+V+RGTF  ILEH I NG ALSQFKTPRCTTN LLL+ILNLST+KTFRS+A
Sbjct: 558 NSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTA 614

[Close]

BLAST of Tan0003143 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 799.3 bits (2063), Expect = 2.2e-231
Identity = 397/599 (66.28%), Postives = 476/599 (79.47%), Query Frame = 0


Query: 3   LEELSAAEEDEEMIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEI 62
           +E + A  +D  +I WFE VS  A   QS TLR+IL+ NSGVEYL++WL     V++ ++
Sbjct: 1   METVEAGHDD--VIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGT---VDVEKM 60

Query: 63  PADDFTFESLFTSLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKY 122
             DD+T E+LFTSLVP+ SH D +PY+QRIADG+++PLLTQ+PI  LSLSSGTT+GRQKY
Sbjct: 61  --DDYTLETLFTSLVPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKY 120

Query: 123 VPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYY 182
           VPFT HSAQTTL IFRL+AAYRSR YP REGG+ILEFIY+ K+ KT GG+T GTATTHYY
Sbjct: 121 VPFTRHSAQTTLQIFRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYY 180

Query: 183 ASDEFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQA 242
           AS+EFK KQ  TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S Q+EFV S F+Y+IVQA
Sbjct: 181 ASEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQA 240

Query: 243 FNELEDTWPQICDDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSE-D 302
           F+  E+ W +IC DI    L S+IT+PK+RKAVL  L  PNP+L S I+ +C EL++   
Sbjct: 241 FSFFEEIWREICADIKEGNLSSRITLPKMRKAVL-ALIRPNPSLASHIEEICLELETNLG 300

Query: 303 WVGLIPKLWPNCKYVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLP 362
           W GLI KLWPN K++ SIMTGSM PY+ KLRHYAGGLPLVSADYGSTESWIGVN+DP LP
Sbjct: 301 WFGLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLP 360

Query: 363 PENVTFAVIPTFSYFEFIPLFRSHDQQN--HHSDFLEGQPVPLSKVKIGQEYEIVLTTFT 422
           PE+V+FAVIPTFSYFEFIPL+R  +Q +     DF+E +PVPLS+VK+GQEYE+VLTTFT
Sbjct: 361 PEDVSFAVIPTFSYFEFIPLYRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFT 420

Query: 423 GLYRCRLGDVVEVAGFHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEES 482
           GLYR RLGDVVEV  FH  TPKL+FI RRKL+LTINIDKNTEKDLQ VV++  Q+L   +
Sbjct: 421 GLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRST 480

Query: 483 GGELVDFTSHAEVATEQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNS 542
             E+VDFTSHA+V   +PGHYVI+WE++G+ DD     CC EMD AFVDYGYVVSRR NS
Sbjct: 481 RAEVVDFTSHADVIA-RPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNS 540

Query: 543 IGPLELRIVQRGTFNSILEHYIRNGAALSQFKTPRCTTNHLLLTILNLSTLKTFRSSAY 599
           IGPLELR+V+RGTF  + E  +     L+QFKTPRCTTN ++L ILN ST+K FRSSAY
Sbjct: 541 IGPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

[Close]

BLAST of Tan0003143 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.7e-157
Identity = 281/586 (47.95%), Postives = 389/586 (66.38%), Query Frame = 0


Query: 15  MIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFESLFT 74
           +ID F++++R A   Q +TL++IL +N    YL+    N    +           E  F 
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDP----------EEAFK 72

Query: 75  SLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQTTL 134
           S+VPL +  + EPY++R+ DGD++P+LT  P+  +SLSSGT+ GR K++PFT    + TL
Sbjct: 73  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 132

Query: 135 LIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQLKT 194
            +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 133 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 192

Query: 195 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQIC 254
            S +CSP EVI   D  Q+ YCHLL G+ F DQ+++V + FA+ +V AF   E  W +I 
Sbjct: 193 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 252

Query: 255 DDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPNCK 314
            DI    L ++ITVP +R A+ K+L TPNP L   I++ C  L   +W GLIP L+PN K
Sbjct: 253 TDIKDGVLSNRITVPSVRTAMSKLL-TPNPELAETIRTKCMSL--SNWYGLIPALFPNAK 312

Query: 315 YVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPTFS 374
           YVY IMTGSM+PYV KLRHYAG LPLVS DYGS+E WI  N+ P L PE  TFAVIP   
Sbjct: 313 YVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLG 372

Query: 375 YFEFIPLFRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVVEVAG 434
           YFEF+P+  + +         E +PV L++VKIG+EYE+V+T + GLYR RLGDVV+V G
Sbjct: 373 YFEFLPVSETGEG--------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIG 432

Query: 435 FHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHAEVAT 494
           F+NNTP+L FICRR L+L+INIDKNTE+DLQL VE   + L EE   E++DF+S+ +V+T
Sbjct: 433 FYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVST 492

Query: 495 EQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQRGTFN 554
           + PGHY IFWE+ G+ ++ V + CC+ +D AF+D GYV SR+  +IG LELR+V +GTF 
Sbjct: 493 D-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFR 552

Query: 555 SILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRSSAY 599
            I EH++  G++  QFK PRC   +N  +L IL  + + ++ S+A+
Sbjct: 553 KIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of Tan0003143 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.7e-157
Identity = 281/586 (47.95%), Postives = 389/586 (66.38%), Query Frame = 0


Query: 15  MIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFESLFT 74
           +ID F++++R A   Q +TL++IL +N    YL+    N    +           E  F 
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDP----------EEAFK 72

Query: 75  SLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQTTL 134
           S+VPL +  + EPY++R+ DGD++P+LT  P+  +SLSSGT+ GR K++PFT    + TL
Sbjct: 73  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 132

Query: 135 LIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQLKT 194
            +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 133 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 192

Query: 195 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQIC 254
            S +CSP EVI   D  Q+ YCHLL G+ F DQ+++V + FA+ +V AF   E  W +I 
Sbjct: 193 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 252

Query: 255 DDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPNCK 314
            DI    L ++ITVP +R A+ K+L TPNP L   I++ C  L   +W GLIP L+PN K
Sbjct: 253 TDIKDGVLSNRITVPSVRTAMSKLL-TPNPELAETIRTKCMSL--SNWYGLIPALFPNAK 312

Query: 315 YVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPTFS 374
           YVY IMTGSM+PYV KLRHYAG LPLVS DYGS+E WI  N+ P L PE  TFAVIP   
Sbjct: 313 YVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLG 372

Query: 375 YFEFIPLFRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVVEVAG 434
           YFEF+P+  + +         E +PV L++VKIG+EYE+V+T + GLYR RLGDVV+V G
Sbjct: 373 YFEFLPVSETGEG--------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIG 432

Query: 435 FHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHAEVAT 494
           F+NNTP+L FICRR L+L+INIDKNTE+DLQL VE   + L EE   E++DF+S+ +V+T
Sbjct: 433 FYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVST 492

Query: 495 EQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQRGTFN 554
           + PGHY IFWE+ G+ ++ V + CC+ +D AF+D GYV SR+  +IG LELR+V +GTF 
Sbjct: 493 D-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFR 552

Query: 555 SILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRSSAY 599
            I EH++  G++  QFK PRC   +N  +L IL  + + ++ S+A+
Sbjct: 553 KIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of Tan0003143 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.7e-157
Identity = 281/586 (47.95%), Postives = 389/586 (66.38%), Query Frame = 0


Query: 15  MIDWFEKVSREAGLAQSRTLRQILQQNSGVEYLKQWLRNDEYVNIHEIPADDFTFESLFT 74
           +ID F++++R A   Q +TL++IL +N    YL+    N    +           E  F 
Sbjct: 24  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDP----------EEAFK 83

Query: 75  SLVPLSSHPDFEPYLQRIADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQTTL 134
           S+VPL +  + EPY++R+ DGD++P+LT  P+  +SLSSGT+ GR K++PFT    + TL
Sbjct: 84  SMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTL 143

Query: 135 LIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQLKT 194
            +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK      
Sbjct: 144 QLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSI 203

Query: 195 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQIC 254
            S +CSP EVI   D  Q+ YCHLL G+ F DQ+++V + FA+ +V AF   E  W +I 
Sbjct: 204 TSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIV 263

Query: 255 DDITHATLCSKITVPKIRKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPNCK 314
            DI    L ++ITVP +R A+ K+L TPNP L   I++ C  L   +W GLIP L+PN K
Sbjct: 264 TDIKDGVLSNRITVPSVRTAMSKLL-TPNPELAETIRTKCMSL--SNWYGLIPALFPNAK 323

Query: 315 YVYSIMTGSMQPYVKKLRHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPTFS 374
           YVY IMTGSM+PYV KLRHYAG LPLVS DYGS+E WI  N+ P L PE  TFAVIP   
Sbjct: 324 YVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLG 383

Query: 375 YFEFIPLFRSHDQQNHHSDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVVEVAG 434
           YFEF+P+  + +         E +PV L++VKIG+EYE+V+T + GLYR RLGDVV+V G
Sbjct: 384 YFEFLPVSETGEG--------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIG 443

Query: 435 FHNNTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHAEVAT 494
           F+NNTP+L FICRR L+L+INIDKNTE+DLQL VE   + L EE   E++DF+S+ +V+T
Sbjct: 444 FYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVST 503

Query: 495 EQPGHYVIFWEVKGDVDDTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQRGTFN 554
           + PGHY IFWE+ G+ ++ V + CC+ +D AF+D GYV SR+  +IG LELR+V +GTF 
Sbjct: 504 D-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFR 563

Query: 555 SILEHYIRNGAALSQFKTPRCT--TNHLLLTILNLSTLKTFRSSAY 599
            I EH++  G++  QFK PRC   +N  +L IL  + + ++ S+A+
Sbjct: 564 KIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

[Close]

BLAST of Tan0003143 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 518.5 bits (1334), Expect = 7.3e-147
Identity = 257/509 (50.49%), Postives = 349/509 (68.57%), Query Frame = 0


Query: 92  IADGDSTPLLTQQPIKTLSLSSGTTDGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPTR 151
           + DGD++P+LT  P+  +SLSSGT+ GR K++PFT    + TL +FR A A+R+R +P  
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 152 EGGKILEFIYSSKQTKTKGGITTGTATTHYYASDEFKIKQLKTKSFTCSPQEVISGGDYK 211
           + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP EVI   D  
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 212 QSTYCHLLLGLFFSDQIEFVTSTFAYSIVQAFNELEDTWPQICDDITHATLCSKITVPKI 271
           Q+ YCHLL G+ F DQ+++V + FA+ +V AF   E  W +I  DI    L ++ITVP +
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 272 RKAVLKVLSTPNPTLGSRIQSLCRELQSEDWVGLIPKLWPNCKYVYSIMTGSMQPYVKKL 331
           R A+ K+L TPNP L   I++ C  L   +W GLIP L+PN KYVY IMTGSM+PYV KL
Sbjct: 181 RTAMSKLL-TPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKL 240

Query: 332 RHYAGGLPLVSADYGSTESWIGVNLDPDLPPENVTFAVIPTFSYFEFIPLFRSHDQQNHH 391
           RHYAG LPLVS DYGS+E WI  N+ P L PE  TFAVIP   YFEF+P+  + +     
Sbjct: 241 RHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPVSETGEG---- 300

Query: 392 SDFLEGQPVPLSKVKIGQEYEIVLTTFTGLYRCRLGDVVEVAGFHNNTPKLNFICRRKLV 451
               E +PV L++VKIG+EYE+V+T + GLYR RLGDVV+V GF+NNTP+L FICRR L+
Sbjct: 301 ----EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLI 360

Query: 452 LTINIDKNTEKDLQLVVERGRQVLGEESGGELVDFTSHAEVATEQPGHYVIFWEVKGDVD 511
           L+INIDKNTE+DLQL VE   + L EE   E++DF+S+ +V+T+ PGHY IFWE+ G+ +
Sbjct: 361 LSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVSTD-PGHYAIFWEISGETN 420

Query: 512 DTVFRRCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVQRGTFNSILEHYIRNGAALSQFK 571
           + V + CC+ +D AF+D GYV SR+  +IG LELR+V +GTF  I EH++  G++  QFK
Sbjct: 421 EDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFK 480

Query: 572 TPRCT--TNHLLLTILNLSTLKTFRSSAY 599
            PRC   +N  +L IL  + + ++ S+A+
Sbjct: 481 MPRCVKPSNAKVLQILCENVVSSYFSTAF 496

The following BLAST results are available for this feature:

BLAST of Tan0003143 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNS2	3.0e-230	66.28	Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A0A1J6KGJ9	5.4e-171	51.19	Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ...	[more]
A0A314KSQ4	3.9e-169	51.08	Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ...	[more]
Q6I581	7.1e-163	49.75	Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SKE2	3.8e-156	47.95	Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ...	[more]

BLAST of Tan0003143 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986272.1	1.5e-290	82.55	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima]	[more]
KAG6570848.1	8.9e-288	81.88	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022943441.1	1.5e-287	81.71	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata]	[more]
XP_023512179.1	2.0e-287	81.88	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo]	[more]
KAG7010696.1	1.4e-285	80.03	Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argy...	[more]

BLAST of Tan0003143 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J741	7.1e-291	82.55	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FRQ1	7.4e-288	81.71	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A5D3B9T4	5.3e-278	79.17	Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BIL6	5.3e-278	79.17	jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1CHT7	2.0e-277	78.57	indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Tan0003143 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03400.1	2.2e-231	66.28	Auxin-responsive GH3 family protein	[more]
AT2G46370.1	2.7e-157	47.95	Auxin-responsive GH3 family protein	[more]
AT2G46370.2	2.7e-157	47.95	Auxin-responsive GH3 family protein	[more]
AT2G46370.4	2.7e-157	47.95	Auxin-responsive GH3 family protein	[more]
AT2G46370.3	7.3e-147	50.49	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 11..437 e-value: 6.1E-146 score: 487.4
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 4..457
None	No IPR available	PANTHER	PTHR31901:SF48	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.10	coord: 4..457

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0003143.1	Tan0003143.1	mRNA
Tan0003143.2	Tan0003143.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity