Tan0003001 (gene) Snake gourd v1

Overview
NameTan0003001
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionethylene-responsive transcription factor CRF4-like
LocationLG01: 1466215 .. 1467876 (-)
RNA-Seq ExpressionTan0003001
SyntenyTan0003001
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACAGCGATTTCAGTATTTTCGATTCCATTGCTATTGCAATTTCAATGGCGTTCTTCTGAGAGATTTCTCAAACAGTTACAGTACTTACTGACCCCATCTCTCTTTCTCTCTCACAATAAATACTTCTTCTTCTTCTTCTTCTTCTTCTTCATCTTCCTCCCCTATTCTCATTAGTCCCACAGAACAGGACTAAGAAACAGCCAGAACAAGCGTGTTCCTCACTGTGCCGTTTTTAGTTTCTTTTTCCTCAGTGTGAATCCGAAAAACGAAAAACTGTCTGTTTTTTGACTTACCCTTTTCCCTCTCCGTCTATAAAATCCCCCATTCCTCTTACAGTTTGATGCTGTATCTCCCATGGAGGATACTCTGTTTTTTCCTGTTAAATTCACAGAGCACCGTAACTTCACCAACAAATTTTCCTCCAACAAACACCACTCATCGGAAAATCGACTCGTACGGATTTCCGTCACCGACCCTGATGCCACGGATTCCTCCAGCGACGAAGAAGGGGAGTTTTTTGAGCGCCAGAGAGTCAAGAAATATGTCAATGAGATCAAAATCCAAAGTGGCTCTCGAAATACCTTTCTACCCACTTGCCGGAAAAGGCCTGCCGGTGAGCCTTCGGATTTACGCCCGCCGGGGAAGCCGGCTCCGCTCACCAATGGGAAGAAATTTCGTGGCGTACGCCAGCGGCCCTGGGGTAAATGGGCGGCCGAGATTAGAGATCCGGCCCGCCGTGTACGGCTGTGGTTGGGAACTTACGACACGGCGGAGGAAGCTGCCATGGTGTACGATAACGCCGCCATTCAGCTTCGGGGGCCGGACGCTTTGACGAACTTTGCAACGCCACCGCCGCCGGTGCCGGAGAAGGAAGTGGAGGCGGCGAATATCCCCTCTGTTTCCGGTTCCTATTACGACTCCGGTGAGGAGTCTCACAATTTGTCATCGCCGACTTCGGTTCTCCACTTCAGAACACCGTCGCTGGAGGAATCCGAAAACCCCCAAAAATCCGACGAGCTTCAGAAACCGCCGCCGCCGCTCGCCGACGAACAATTTCACGAATGTCAAGGGGAAACTTCATTTTCCGAGGAGTATTACACCGATTTCCCTAAATTCGACGACGTTTTCAAGCTTCCGATCCCCGAGTCGCCGATCTTCTTCGACGAACAGCCGCTGTTTTTCGAAGAAAGCATATTTAACGAAGGTTTCAGTGAAATGTTTACAAATTTGCCTGAAGATTTTGGGTCGCCATTGTTGTCTTCTTCGATCGGTCAAGGGGCCGATGACTATTTCCAAGATATTTTGATGGGGTCGGATCCTCTAGTAGTTCTGTAAGCGACGCGGCGGAGATCCGGCCGGCCGGCGGCGGTGGACCACTGCTCCCACCGTTATGTCGGCGGAATTTTGTTCCCATATCGCATCTCTCAAAAAACGGCTATTTTTGCGACTTTTCCTTTTCTTTTTCTTTTTTTTTTGGTATACTTTTCTTTACCTGTTTACATATTCTGGGAAGTTGAGTGGACCAGTTTTGCTAGAGATATTTATTCTTTTCCCCCCTTTTTCCTTTTTTTATTTAAATCAATAATTAGGCTTTGTTATTATTTATTAAGAGTGTATAATGTGGAAGAGCTTTGTATAATTATATATCTCAAATATTTT

mRNA sequence

AAAACAGCGATTTCAGTATTTTCGATTCCATTGCTATTGCAATTTCAATGGCGTTCTTCTGAGAGATTTCTCAAACAGTTACAGTACTTACTGACCCCATCTCTCTTTCTCTCTCACAATAAATACTTCTTCTTCTTCTTCTTCTTCTTCTTCATCTTCCTCCCCTATTCTCATTAGTCCCACAGAACAGGACTAAGAAACAGCCAGAACAAGCGTGTTCCTCACTGTGCCGTTTTTAGTTTCTTTTTCCTCAGTGTGAATCCGAAAAACGAAAAACTGTCTGTTTTTTGACTTACCCTTTTCCCTCTCCGTCTATAAAATCCCCCATTCCTCTTACAGTTTGATGCTGTATCTCCCATGGAGGATACTCTGTTTTTTCCTGTTAAATTCACAGAGCACCGTAACTTCACCAACAAATTTTCCTCCAACAAACACCACTCATCGGAAAATCGACTCGTACGGATTTCCGTCACCGACCCTGATGCCACGGATTCCTCCAGCGACGAAGAAGGGGAGTTTTTTGAGCGCCAGAGAGTCAAGAAATATGTCAATGAGATCAAAATCCAAAGTGGCTCTCGAAATACCTTTCTACCCACTTGCCGGAAAAGGCCTGCCGGTGAGCCTTCGGATTTACGCCCGCCGGGGAAGCCGGCTCCGCTCACCAATGGGAAGAAATTTCGTGGCGTACGCCAGCGGCCCTGGGGTAAATGGGCGGCCGAGATTAGAGATCCGGCCCGCCGTGTACGGCTGTGGTTGGGAACTTACGACACGGCGGAGGAAGCTGCCATGGTGTACGATAACGCCGCCATTCAGCTTCGGGGGCCGGACGCTTTGACGAACTTTGCAACGCCACCGCCGCCGGTGCCGGAGAAGGAAGTGGAGGCGGCGAATATCCCCTCTGTTTCCGGTTCCTATTACGACTCCGGTGAGGAGTCTCACAATTTGTCATCGCCGACTTCGGTTCTCCACTTCAGAACACCGTCGCTGGAGGAATCCGAAAACCCCCAAAAATCCGACGAGCTTCAGAAACCGCCGCCGCCGCTCGCCGACGAACAATTTCACGAATGTCAAGGGGAAACTTCATTTTCCGAGGAGTATTACACCGATTTCCCTAAATTCGACGACGTTTTCAAGCTTCCGATCCCCGAGTCGCCGATCTTCTTCGACGAACAGCCGCTGTTTTTCGAAGAAAGCATATTTAACGAAGGTTTCAGTGAAATGTTTACAAATTTGCCTGAAGATTTTGGGTCGCCATTGTTGTCTTCTTCGATCGGTCAAGGGGCCGATGACTATTTCCAAGATATTTTGATGGGGTCGGATCCTCTAGTAGTTCTGTAAGCGACGCGGCGGAGATCCGGCCGGCCGGCGGCGGTGGACCACTGCTCCCACCGTTATGTCGGCGGAATTTTGTTCCCATATCGCATCTCTCAAAAAACGGCTATTTTTGCGACTTTTCCTTTTCTTTTTCTTTTTTTTTTGGTATACTTTTCTTTACCTGTTTACATATTCTGGGAAGTTGAGTGGACCAGTTTTGCTAGAGATATTTATTCTTTTCCCCCCTTTTTCCTTTTTTTATTTAAATCAATAATTAGGCTTTGTTATTATTTATTAAGAGTGTATAATGTGGAAGAGCTTTGTATAATTATATATCTCAAATATTTT

Coding sequence (CDS)

ATGGAGGATACTCTGTTTTTTCCTGTTAAATTCACAGAGCACCGTAACTTCACCAACAAATTTTCCTCCAACAAACACCACTCATCGGAAAATCGACTCGTACGGATTTCCGTCACCGACCCTGATGCCACGGATTCCTCCAGCGACGAAGAAGGGGAGTTTTTTGAGCGCCAGAGAGTCAAGAAATATGTCAATGAGATCAAAATCCAAAGTGGCTCTCGAAATACCTTTCTACCCACTTGCCGGAAAAGGCCTGCCGGTGAGCCTTCGGATTTACGCCCGCCGGGGAAGCCGGCTCCGCTCACCAATGGGAAGAAATTTCGTGGCGTACGCCAGCGGCCCTGGGGTAAATGGGCGGCCGAGATTAGAGATCCGGCCCGCCGTGTACGGCTGTGGTTGGGAACTTACGACACGGCGGAGGAAGCTGCCATGGTGTACGATAACGCCGCCATTCAGCTTCGGGGGCCGGACGCTTTGACGAACTTTGCAACGCCACCGCCGCCGGTGCCGGAGAAGGAAGTGGAGGCGGCGAATATCCCCTCTGTTTCCGGTTCCTATTACGACTCCGGTGAGGAGTCTCACAATTTGTCATCGCCGACTTCGGTTCTCCACTTCAGAACACCGTCGCTGGAGGAATCCGAAAACCCCCAAAAATCCGACGAGCTTCAGAAACCGCCGCCGCCGCTCGCCGACGAACAATTTCACGAATGTCAAGGGGAAACTTCATTTTCCGAGGAGTATTACACCGATTTCCCTAAATTCGACGACGTTTTCAAGCTTCCGATCCCCGAGTCGCCGATCTTCTTCGACGAACAGCCGCTGTTTTTCGAAGAAAGCATATTTAACGAAGGTTTCAGTGAAATGTTTACAAATTTGCCTGAAGATTTTGGGTCGCCATTGTTGTCTTCTTCGATCGGTCAAGGGGCCGATGACTATTTCCAAGATATTTTGATGGGGTCGGATCCTCTAGTAGTTCTGTAA

Protein sequence

MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRVKKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPVPEKEVEAANIPSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQGETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSPLLSSSIGQGADDYFQDILMGSDPLVVL
Homology
BLAST of Tan0003001 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 2.6e-50
Identity = 137/331 (41.39%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 8   PVKFTEH----RNFTNKFSSNKHHSSEN--RLVRISVTDPDATDSSSDEEGEFFERQRVK 67
           PVK+TEH    R +T K S  +  S  +  RLVR+S+TD DATDSSSDEE   F R+RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 68  KYVNEIKIQSGSRNT----FLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGK 127
           + +NEI+++  S +T      PT  ++     S ++ P       N KK+RGVRQRPWGK
Sbjct: 71  RLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVSG--QNQKKYRGVRQRPWGK 130

Query: 128 WAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPVPEKEVEAA 187
           WAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF   P P P +E E  
Sbjct: 131 WAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQE 190

Query: 188 NIPSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESEN----PQKSDELQKPPPPLADEQ 247
              ++S S  +S ++S +LSSPTSVL+++T   EE  +    P K + L+    P++   
Sbjct: 191 PESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQEPIS--- 250

Query: 248 FHECQGETSFSEEYY-TDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMF--- 307
           +H  +G T+ +++ +  D    D+ F   +P+  IF  +QP+   +   N+ F+++    
Sbjct: 251 WHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLMLFD 310

Query: 308 TNLPEDFGSPL--LSSSIGQGADDYFQDILM 319
           +N  E + S +  + SS     D    D+L+
Sbjct: 311 SNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

BLAST of Tan0003001 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.8e-47
Identity = 158/352 (44.89%), Postives = 201/352 (57.10%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGE-----FFERQRVKKY 68
           +KFTEH+  T    S   ++ + R++RISVTDPDATDSSSD+E E       +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  VNEIKIQSGSRNTFLPTC--------RKRPAGEPSDLRPPGKPAPLTNG-KKFRGVRQRP 128
           VNE+ + SG+  T   +C        +KR     S + P       T G KKFRGVRQRP
Sbjct: 72  VNEVYLDSGAVVT--GSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRP 131

Query: 129 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA-TP-------- 188
           WGKWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+ TP        
Sbjct: 132 WGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKA 191

Query: 189 PPPVPEKEVEAAN---IPSVSGSYYDSGEESHN-LSSPTSVLHFRTP-SLEESENPQKSD 248
           PPP P K+ +  N     SV+ S   S   S++ L SP SVL  R+P +++E      S 
Sbjct: 192 PPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVL--RSPFAVDEFSGISSS- 251

Query: 249 ELQKPPPPLADEQFHECQGETSFSEEYYTDF--PKF--DDVFKLPIPESPIFFDEQPLFF 308
                  P+A     E    T+ SE  ++DF  P F  DDVF       P +     LF 
Sbjct: 252 -------PVAAVVVKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGGD-LFG 311

Query: 309 EESIFNEGFSEMFTNLPEDFGSPLLSSSIGQGADDYFQDI--LMGSDPLVVL 327
           E+    +  ++M  N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 EDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of Tan0003001 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-46
Identity = 129/322 (40.06%), Postives = 172/322 (53.42%), Query Frame = 0

Query: 6   FFPVKFTEHR----NFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGE--FFERQR 65
           F P+K+TEH+     +T K S         + V I  TDPDATDSSSDE+ E   F R+R
Sbjct: 7   FRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRR 66

Query: 66  VKKYVNEIKIQSGSRNTFLPTC---RKRPAGEPSDLRPPGKPAPLTNG--------KKFR 125
           VK++VNEI ++    N         RKR +    + + P       N         KKFR
Sbjct: 67  VKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKKFR 126

Query: 126 GVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP-- 185
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+ PP  
Sbjct: 127 GVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPPQE 186

Query: 186 --------PPVPEKEVEAANIPSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQK 245
                   P + EK V        + S   + E+  +LSSPTSVL+ R+  +++ + P K
Sbjct: 187 EEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQPFK 246

Query: 246 SDELQKPPPPLADEQFHECQGETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEE 301
           S    KP P +++  +      T   E         DD F L  P    +F+E P   E 
Sbjct: 247 S---AKPEPGVSNAPWWHTGFNTGLGES--------DDSFPLDTPFLDNYFNESPP--EM 306

BLAST of Tan0003001 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-38
Identity = 133/314 (42.36%), Postives = 167/314 (53.18%), Query Frame = 0

Query: 32  RLVRISVTDPDATDSSSDEEGEF------FERQRVKKYVNEIKIQSGSRNTFLPTCRKRP 91
           R++RISVTDP ATDSSSDEE E        +R+RVKKYV E+ + S      + + +++P
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDS------VVSDKEKP 70

Query: 92  AGEPSDLRPPGKPAPLTNG-KKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAM 151
             +    R    P  +T   +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEAA+
Sbjct: 71  MKKKRKKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAI 130

Query: 152 VYDNAAIQLRGPDALTNFATPPPPVPEKEVEAA-NIPSVSGSYYDSGEESHNLSSPTSVL 211
           VYDNAAIQLRGP+A  NF  PPPPV E   EA+  +  VS      GE    L SP SVL
Sbjct: 131 VYDNAAIQLRGPNAELNF--PPPPVTENVEEASTEVKGVSDFIIGGGE---CLRSPVSVL 190

Query: 212 HF-----RTPSLEESENPQKSDELQKPPPPLADEQFHECQGETSFSEEYYTDFPKFDDVF 271
                   T   EE      ++ + K  P             + FS   ++D    DDVF
Sbjct: 191 ESPFSGESTAVKEEFVGVSTAEIVVKKEPSF---------NGSDFSAPLFSD----DDVF 250

Query: 272 KLPIPESPIFFDEQPLF----FEESIFNEGFSEMFTNLPEDFGSPLLSSSIGQGADDYFQ 327
                 S  F  +  LF    F +  F  GF          FGS   S       +D+FQ
Sbjct: 251 GFSTSMSESFGGD--LFGDNLFADMSFGSGFG---------FGSG--SGFSSWHVEDHFQ 287

BLAST of Tan0003001 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.1e-35
Identity = 122/313 (38.98%), Postives = 161/313 (51.44%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEE--GEFFERQRVKKYVNE 68
           VKFTE+R  TN  ++  + S   RLVRI+VTDP ATDSSSD++         RVK+YV E
Sbjct: 8   VKFTENRTVTNVAATPSNGSP--RLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKE 67

Query: 69  IKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAAEIRDPA 128
           I+   G  ++     RK    E   +      +     KK+RGVRQRPWGK+AAEIRDP+
Sbjct: 68  IRFCQGESSS-STAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPS 127

Query: 129 RRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPVPEKEVEAANIPSVSGSY 188
            R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF TPP P P  +++         S 
Sbjct: 128 SRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTV-------SA 187

Query: 189 YDSGEES-HNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQGETSFSE 248
            D G +S  +L SPTSVL F     EE+E+  ++ EL         ++  E      F +
Sbjct: 188 CDYGRDSRQSLHSPTSVLRFNVN--EETEHEIEAIELSPERKSTVIKEEEESSAGLVFPD 247

Query: 249 EY-YTDFPKFDDVF-KLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSPLLSS 308
            Y   D     + F    I    +F DE+       + N   S+   N  EDF   L+  
Sbjct: 248 PYLLPDLSLAGECFWDTEIAPDLLFLDEETKIQSTLLPNTEVSKQGENETEDFEFGLIDD 307

Query: 309 SIGQGAD-DYFQD 316
                 D D+F D
Sbjct: 308 FESSPWDVDHFFD 308

BLAST of Tan0003001 vs. NCBI nr
Match: XP_004149454.1 (ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypothetical protein Csa_011137 [Cucumis sativus])

HSP 1 Score: 551.2 bits (1419), Expect = 6.0e-153
Identity = 275/327 (84.10%), Postives = 297/327 (90.83%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVKFTEHRNFTNKFS+ KHH SE+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN  LP+CRKRPAG+ S+ R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
           PSVSGSYYDS EESHNLSSPTSVLHFRTPS EESE P KSD+LQKPP P  D+QFHECQG
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQG 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           ETSF+EEY+T+FP+FD  FK P PE+PIF D+QPLFFE+SI+N+ FSE+FTNLPEDFGSP
Sbjct: 241 ETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSP 300

Query: 301 LLSSSIGQGADDYFQDILMGSDPLVVL 327
           LLSSSIGQG DDYFQDILMGSDPLVVL
Sbjct: 301 LLSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of Tan0003001 vs. NCBI nr
Match: XP_038900136.1 (ethylene-responsive transcription factor CRF4-like [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 274/327 (83.79%), Postives = 294/327 (89.91%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVKFTEHRNFTNKFS+ KHH SE+R+VRISVTDPDATDSSSDE+ ++FERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHHSESRVVRISVTDPDATDSSSDED-DYFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN  LP+CRKRPAGE SD +  GK AP  NGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGERSDFQRQGKVAPPMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP  +PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVSMPEKEVEMANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
           PSVSGSYYDS EESHNLSSPTSVLHFRTPS EESE P KSDE+Q PP P  D+QFHECQG
Sbjct: 181 PSVSGSYYDSCEESHNLSSPTSVLHFRTPSPEESEKPPKSDEVQNPPAPFTDDQFHECQG 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           ETSFSEE++T+FP+FD  FK P PESPIF D+QPLFFE+SI+NE FSE+FTNLPEDFGSP
Sbjct: 241 ETSFSEEFHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNEDFSEIFTNLPEDFGSP 300

Query: 301 LLSSSIGQGADDYFQDILMGSDPLVVL 327
           LLSSSI QG DDYFQDILMGSDPLVVL
Sbjct: 301 LLSSSISQGGDDYFQDILMGSDPLVVL 326

BLAST of Tan0003001 vs. NCBI nr
Match: XP_008439327.1 (PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KAA0033741.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa] >TYJ99880.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa])

HSP 1 Score: 542.0 bits (1395), Expect = 3.6e-150
Identity = 274/328 (83.54%), Postives = 294/328 (89.63%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVKFTEHRNFTNKFS+ KHH SE+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN  LP+CRKRPAG+ S+ R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP  P+PEKEVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
           PSVSGSYYDS EESHNLSSPTSVLHFRTPS EESE P KSD+LQKPP P  D+QFHECQG
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQG 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           ETSFSEEY+T+FP+FD  FK P PESPIF D+QPLFFE+SI+N+ FSE+FTNLPEDFGSP
Sbjct: 241 ETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSP 300

Query: 301 LL-SSSIGQGADDYFQDILMGSDPLVVL 327
           LL SSSIGQG DDYFQDIL GSDPLVVL
Sbjct: 301 LLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of Tan0003001 vs. NCBI nr
Match: KAG6576679.1 (Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014728.1 Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538.9 bits (1387), Expect = 3.1e-149
Identity = 274/327 (83.79%), Postives = 290/327 (88.69%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVK TEHRNFT+KFS  KHH SE+R+VRISVTDPDATDSSSDEE E FERQRV
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKKHHQSESRVVRISVTDPDATDSSSDEE-ELFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN+ LP+CRKR AGE SD R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP   P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKEVEVANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
            SVSGSYYDS EESHNLSSPTSVLHFRTPS EE+E PQK DE Q+PPPP AD+QFHECQ 
Sbjct: 181 ASVSGSYYDSSEESHNLSSPTSVLHFRTPSPEETEKPQKPDESQQPPPPFADDQFHECQV 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           +T+ SEEYYT+FP+FDDVFKLPIPE PIF DEQPLFFEESI+NE FSE FTNLPEDFG+P
Sbjct: 241 KTTLSEEYYTEFPRFDDVFKLPIPEPPIFVDEQPLFFEESIWNEDFSEFFTNLPEDFGTP 300

Query: 301 LLSSSIGQGADDYFQDILMGSDPLVVL 327
           LLSS I QG DDYFQDILMGSDPLVVL
Sbjct: 301 LLSSPISQGGDDYFQDILMGSDPLVVL 326

BLAST of Tan0003001 vs. NCBI nr
Match: XP_023552792.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.3 bits (1383), Expect = 8.9e-149
Identity = 273/327 (83.49%), Postives = 290/327 (88.69%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVK TEHRNFT+KFS  K+H SE+R+VRISVTDPDATDSSSDEE E FERQRV
Sbjct: 1   MEDTLFCPVKLTEHRNFTSKFSLKKYHQSESRVVRISVTDPDATDSSSDEE-ELFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN+ LP+CRKR AGE SD R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP P P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSPVPMPEKEVEVANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
            S SGSYYDS EESHNLSSPTSVLHFRTPS EE+E PQK DE Q+PPPP AD+QFHECQ 
Sbjct: 181 ASGSGSYYDSSEESHNLSSPTSVLHFRTPSPEETEKPQKPDESQQPPPPFADDQFHECQV 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           +T+ S EYYT+FP+FDDVFKLPIPESPIF DEQPLFFEESI+NE FSE FTNLPEDFG+P
Sbjct: 241 KTTISSEYYTEFPRFDDVFKLPIPESPIFVDEQPLFFEESIWNEDFSEFFTNLPEDFGTP 300

Query: 301 LLSSSIGQGADDYFQDILMGSDPLVVL 327
           LLSS I QG DDYFQDILMGSDPLVVL
Sbjct: 301 LLSSPISQGGDDYFQDILMGSDPLVVL 326

BLAST of Tan0003001 vs. ExPASy TrEMBL
Match: A0A0A0LB30 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.9e-153
Identity = 275/327 (84.10%), Postives = 297/327 (90.83%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVKFTEHRNFTNKFS+ KHH SE+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN  LP+CRKRPAG+ S+ R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
           PSVSGSYYDS EESHNLSSPTSVLHFRTPS EESE P KSD+LQKPP P  D+QFHECQG
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQG 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           ETSF+EEY+T+FP+FD  FK P PE+PIF D+QPLFFE+SI+N+ FSE+FTNLPEDFGSP
Sbjct: 241 ETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSP 300

Query: 301 LLSSSIGQGADDYFQDILMGSDPLVVL 327
           LLSSSIGQG DDYFQDILMGSDPLVVL
Sbjct: 301 LLSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of Tan0003001 vs. ExPASy TrEMBL
Match: A0A5D3BJ86 (Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold63471G00010 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.8e-150
Identity = 274/328 (83.54%), Postives = 294/328 (89.63%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVKFTEHRNFTNKFS+ KHH SE+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN  LP+CRKRPAG+ S+ R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP  P+PEKEVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
           PSVSGSYYDS EESHNLSSPTSVLHFRTPS EESE P KSD+LQKPP P  D+QFHECQG
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQG 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           ETSFSEEY+T+FP+FD  FK P PESPIF D+QPLFFE+SI+N+ FSE+FTNLPEDFGSP
Sbjct: 241 ETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSP 300

Query: 301 LL-SSSIGQGADDYFQDILMGSDPLVVL 327
           LL SSSIGQG DDYFQDIL GSDPLVVL
Sbjct: 301 LLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of Tan0003001 vs. ExPASy TrEMBL
Match: A0A1S3AY55 (ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LOC103484149 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.8e-150
Identity = 274/328 (83.54%), Postives = 294/328 (89.63%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVKFTEHRNFTNKFS+ KHH SE+R+VRISVTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN  LP+CRKRPAG+ S+ R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP  P+PEKEVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
           PSVSGSYYDS EESHNLSSPTSVLHFRTPS EESE P KSD+LQKPP P  D+QFHECQG
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQG 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           ETSFSEEY+T+FP+FD  FK P PESPIF D+QPLFFE+SI+N+ FSE+FTNLPEDFGSP
Sbjct: 241 ETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSP 300

Query: 301 LL-SSSIGQGADDYFQDILMGSDPLVVL 327
           LL SSSIGQG DDYFQDIL GSDPLVVL
Sbjct: 301 LLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of Tan0003001 vs. ExPASy TrEMBL
Match: A0A6J1E4E1 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662 GN=LOC111430503 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.1e-147
Identity = 272/327 (83.18%), Postives = 288/327 (88.07%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGEFFERQRV 60
           MEDTLF PVK TEHRNFT+KFS  KHH SE+R+VRISVTDPDATDSSSDEE E FERQRV
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKKHHQSESRVVRISVTDPDATDSSSDEE-ELFERQRV 60

Query: 61  KKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGKWAA 120
           KKY+NEIKIQSG RN+ LP+CRKR AGE SD R  GK  P TNGKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPP-PVPEKEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP   P+PEKEVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKEVEVANI 180

Query: 181 PSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKPPPPLADEQFHECQG 240
            SVSGSYYDS EESHNLSSPTSVLHFRT S EE+E PQK DE Q+PPPP AD+QFHE Q 
Sbjct: 181 ASVSGSYYDSSEESHNLSSPTSVLHFRTSSPEETEKPQKPDESQQPPPPFADDQFHERQV 240

Query: 241 ETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLPEDFGSP 300
           +T+ SEEYYT+FP+FDDVFKLPIPE PIF DEQPLFFEESI+NE FSE FTNLPEDFG+P
Sbjct: 241 KTTLSEEYYTEFPRFDDVFKLPIPEPPIFVDEQPLFFEESIWNEDFSEFFTNLPEDFGTP 300

Query: 301 LLSSSIGQGADDYFQDILMGSDPLVVL 327
           LLSS I QG DDYFQDILMGSDPLVVL
Sbjct: 301 LLSSPISQGGDDYFQDILMGSDPLVVL 326

BLAST of Tan0003001 vs. ExPASy TrEMBL
Match: A0A6J1CH70 (ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=3673 GN=LOC111011618 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.2e-146
Identity = 272/333 (81.68%), Postives = 294/333 (88.29%), Query Frame = 0

Query: 1   MEDTLFFPVKFTEHRNFTNKFSS-----NKHHSSENRLVRISVTDPDATDSSSD-EEGEF 60
           MEDTLF PVKFTEHRNFT KFS+     N+HH  ENR+VRISVTDPDATDSSSD EEGEF
Sbjct: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60

Query: 61  FERQRVKKYVNEIKIQSGSRNTFLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRP 120
           FERQRVKKYVNEIKI+SGSRN+ +P CRKRPAGE S+ R P KP P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPVPEKEV 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPPPP PEKE 
Sbjct: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPP-PEKEA 180

Query: 181 EAANIPSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQKSDELQKP-PPPLADEQ 240
           E  NIPSVSGSYYDSGEESHNLSSPTSVLHFRT S EESENP K +  QKP PP LAD+Q
Sbjct: 181 E-VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQ 240

Query: 241 FHECQGETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMFTNLP 300
           FHECQGETSFS EY TDFPKF+D+F+LP P+  IFFDEQPLFFEESI+NEGFS++FTN+P
Sbjct: 241 FHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMP 300

Query: 301 EDFGSPLLSSSIGQGADDYFQDILMGSDPLVVL 327
           ED GSP+LSSSI QG DDYFQDIL+GSDPLVVL
Sbjct: 301 EDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 331

BLAST of Tan0003001 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 200.7 bits (509), Expect = 1.8e-51
Identity = 137/331 (41.39%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 8   PVKFTEH----RNFTNKFSSNKHHSSEN--RLVRISVTDPDATDSSSDEEGEFFERQRVK 67
           PVK+TEH    R +T K S  +  S  +  RLVR+S+TD DATDSSSDEE   F R+RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 68  KYVNEIKIQSGSRNT----FLPTCRKRPAGEPSDLRPPGKPAPLTNGKKFRGVRQRPWGK 127
           + +NEI+++  S +T      PT  ++     S ++ P       N KK+RGVRQRPWGK
Sbjct: 71  RLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVSG--QNQKKYRGVRQRPWGK 130

Query: 128 WAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPPVPEKEVEAA 187
           WAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF   P P P +E E  
Sbjct: 131 WAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQE 190

Query: 188 NIPSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESEN----PQKSDELQKPPPPLADEQ 247
              ++S S  +S ++S +LSSPTSVL+++T   EE  +    P K + L+    P++   
Sbjct: 191 PESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQEPIS--- 250

Query: 248 FHECQGETSFSEEYY-TDFPKFDDVFKLPIPESPIFFDEQPLFFEESIFNEGFSEMF--- 307
           +H  +G T+ +++ +  D    D+ F   +P+  IF  +QP+   +   N+ F+++    
Sbjct: 251 WHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLMLFD 310

Query: 308 TNLPEDFGSPL--LSSSIGQGADDYFQDILM 319
           +N  E + S +  + SS     D    D+L+
Sbjct: 311 SNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

BLAST of Tan0003001 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 189.1 bits (479), Expect = 5.5e-48
Identity = 158/352 (44.89%), Postives = 201/352 (57.10%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGE-----FFERQRVKKY 68
           +KFTEH+  T    S   ++ + R++RISVTDPDATDSSSD+E E       +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  VNEIKIQSGSRNTFLPTC--------RKRPAGEPSDLRPPGKPAPLTNG-KKFRGVRQRP 128
           VNE+ + SG+  T   +C        +KR     S + P       T G KKFRGVRQRP
Sbjct: 72  VNEVYLDSGAVVT--GSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRP 131

Query: 129 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA-TP-------- 188
           WGKWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+ TP        
Sbjct: 132 WGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKA 191

Query: 189 PPPVPEKEVEAAN---IPSVSGSYYDSGEESHN-LSSPTSVLHFRTP-SLEESENPQKSD 248
           PPP P K+ +  N     SV+ S   S   S++ L SP SVL  R+P +++E      S 
Sbjct: 192 PPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVL--RSPFAVDEFSGISSS- 251

Query: 249 ELQKPPPPLADEQFHECQGETSFSEEYYTDF--PKF--DDVFKLPIPESPIFFDEQPLFF 308
                  P+A     E    T+ SE  ++DF  P F  DDVF       P +     LF 
Sbjct: 252 -------PVAAVVVKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGGD-LFG 311

Query: 309 EESIFNEGFSEMFTNLPEDFGSPLLSSSIGQGADDYFQDI--LMGSDPLVVL 327
           E+    +  ++M  N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 EDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of Tan0003001 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 189.1 bits (479), Expect = 5.5e-48
Identity = 158/352 (44.89%), Postives = 201/352 (57.10%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGE-----FFERQRVKKY 68
           +KFTEH+  T    S   ++ + R++RISVTDPDATDSSSD+E E       +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  VNEIKIQSGSRNTFLPTC--------RKRPAGEPSDLRPPGKPAPLTNG-KKFRGVRQRP 128
           VNE+ + SG+  T   +C        +KR     S + P       T G KKFRGVRQRP
Sbjct: 72  VNEVYLDSGAVVT--GSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRP 131

Query: 129 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA-TP-------- 188
           WGKWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+ TP        
Sbjct: 132 WGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKA 191

Query: 189 PPPVPEKEVEAAN---IPSVSGSYYDSGEESHN-LSSPTSVLHFRTP-SLEESENPQKSD 248
           PPP P K+ +  N     SV+ S   S   S++ L SP SVL  R+P +++E      S 
Sbjct: 192 PPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVL--RSPFAVDEFSGISSS- 251

Query: 249 ELQKPPPPLADEQFHECQGETSFSEEYYTDF--PKF--DDVFKLPIPESPIFFDEQPLFF 308
                  P+A     E    T+ SE  ++DF  P F  DDVF       P +     LF 
Sbjct: 252 -------PVAAVVVKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGGD-LFG 311

Query: 309 EESIFNEGFSEMFTNLPEDFGSPLLSSSIGQGADDYFQDI--LMGSDPLVVL 327
           E+    +  ++M  N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 EDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of Tan0003001 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 129/322 (40.06%), Postives = 172/322 (53.42%), Query Frame = 0

Query: 6   FFPVKFTEHR----NFTNKFSSNKHHSSENRLVRISVTDPDATDSSSDEEGE--FFERQR 65
           F P+K+TEH+     +T K S         + V I  TDPDATDSSSDE+ E   F R+R
Sbjct: 7   FRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRR 66

Query: 66  VKKYVNEIKIQSGSRNTFLPTC---RKRPAGEPSDLRPPGKPAPLTNG--------KKFR 125
           VK++VNEI ++    N         RKR +    + + P       N         KKFR
Sbjct: 67  VKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKKFR 126

Query: 126 GVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP-- 185
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+ PP  
Sbjct: 127 GVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPPQE 186

Query: 186 --------PPVPEKEVEAANIPSVSGSYYDSGEESHNLSSPTSVLHFRTPSLEESENPQK 245
                   P + EK V        + S   + E+  +LSSPTSVL+ R+  +++ + P K
Sbjct: 187 EEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQPFK 246

Query: 246 SDELQKPPPPLADEQFHECQGETSFSEEYYTDFPKFDDVFKLPIPESPIFFDEQPLFFEE 301
           S    KP P +++  +      T   E         DD F L  P    +F+E P   E 
Sbjct: 247 S---AKPEPGVSNAPWWHTGFNTGLGES--------DDSFPLDTPFLDNYFNESPP--EM 306

BLAST of Tan0003001 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 161.0 bits (406), Expect = 1.6e-39
Identity = 133/314 (42.36%), Postives = 167/314 (53.18%), Query Frame = 0

Query: 32  RLVRISVTDPDATDSSSDEEGEF------FERQRVKKYVNEIKIQSGSRNTFLPTCRKRP 91
           R++RISVTDP ATDSSSDEE E        +R+RVKKYV E+ + S      + + +++P
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDS------VVSDKEKP 70

Query: 92  AGEPSDLRPPGKPAPLTNG-KKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAM 151
             +    R    P  +T   +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEAA+
Sbjct: 71  MKKKRKKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAI 130

Query: 152 VYDNAAIQLRGPDALTNFATPPPPVPEKEVEAA-NIPSVSGSYYDSGEESHNLSSPTSVL 211
           VYDNAAIQLRGP+A  NF  PPPPV E   EA+  +  VS      GE    L SP SVL
Sbjct: 131 VYDNAAIQLRGPNAELNF--PPPPVTENVEEASTEVKGVSDFIIGGGE---CLRSPVSVL 190

Query: 212 HF-----RTPSLEESENPQKSDELQKPPPPLADEQFHECQGETSFSEEYYTDFPKFDDVF 271
                   T   EE      ++ + K  P             + FS   ++D    DDVF
Sbjct: 191 ESPFSGESTAVKEEFVGVSTAEIVVKKEPSF---------NGSDFSAPLFSD----DDVF 250

Query: 272 KLPIPESPIFFDEQPLF----FEESIFNEGFSEMFTNLPEDFGSPLLSSSIGQGADDYFQ 327
                 S  F  +  LF    F +  F  GF          FGS   S       +D+FQ
Sbjct: 251 GFSTSMSESFGGD--LFGDNLFADMSFGSGFG---------FGSG--SGFSSWHVEDHFQ 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUE32.6e-5041.39Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUQ27.8e-4744.89Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FK123.9e-4640.06Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825032.3e-3842.36Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M3743.1e-3538.98Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
XP_004149454.16.0e-15384.10ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypo... [more]
XP_038900136.11.6e-15083.79ethylene-responsive transcription factor CRF4-like [Benincasa hispida][more]
XP_008439327.13.6e-15083.54PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KA... [more]
KAG6576679.13.1e-14983.79Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma s... [more]
XP_023552792.18.9e-14983.49ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LB302.9e-15384.10AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=... [more]
A0A5D3BJ861.8e-15083.54Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3AY551.8e-15083.54ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1E4E13.1e-14783.18ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1CH701.2e-14681.68ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=367... [more]
Match NameE-valueIdentityDescription
AT4G27950.11.8e-5141.39cytokinin response factor 4 [more]
AT4G23750.15.5e-4844.89cytokinin response factor 2 [more]
AT4G23750.25.5e-4844.89cytokinin response factor 2 [more]
AT5G53290.12.8e-4740.06cytokinin response factor 3 [more]
AT4G11140.11.6e-3942.36cytokinin response factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 107..118
score: 70.91
coord: 129..145
score: 61.5
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 106..169
e-value: 7.3E-39
score: 145.1
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 106..155
e-value: 8.4E-14
score: 51.6
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 106..163
score: 23.762146
IPR001471AP2/ERF domainCDDcd00018AP2coord: 105..163
e-value: 7.67256E-34
score: 116.987
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 105..164
e-value: 3.7E-26
score: 93.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..210
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..107
NoneNo IPR availablePANTHERPTHR31194:SF137ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF4-LIKEcoord: 6..217
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 6..217
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 106..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003001.1Tan0003001.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity