Tan0002865 (gene) Snake gourd v1

Overview


Name	Tan0002865
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Cytochrome p450 76c2
Location	LG07: 5854218 .. 5857416 (+)
RNA-Seq Expression	Tan0002865
Synteny	Tan0002865

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCAGTAAATTTCAAAAATCACAACTAAAACTTAAAATGGAACTCTATTTTTTTTTATTAAATTTCTTATTATTGAACAAACATCCAAAATAACTTGTCTTTTTATCGATTTATTTTCTATATTTATATAATAAATGATGTATGAAGATTGAAACTCTAATTTTAAAATAAGGAATAAATGCTAATTATCATTAACACTATTGATACAAAAATTTAATTAATATTTCTATAATATCGGAGTTGACAAAGTAAATAGTGTCAACAAAATATTACTAATATAATATCAACCTAATATTTATTAAAAAAACTTTTAACTGGTTTAAAAAGTGTTTAATATTTATTTCTTTATTTAAATATGCGTTTAATAAATTTCTTGATTTGTATATCTTTTCAAAAATATCTATTGACAACTTGAAATCCACATATTTTTTTTATCCTACACTAATTATATATTTAATAATTTTAGATTAAAGGACAAATTTAATCTCTAAACTTTGAAGTATATGTGTATTTGTTCTTTGACCTTTCAAAACTTTAAATTTAGTCTTTGAACATTTGAATTTTTTAAAATTTACGAATGTACTATACAAAAATTTAGATTTATATCTAATAATCAATTAGTTTTTTTTCCAACAATAACATAACTCATCTGACATCACGTATAATTCATAACCAAGAGGTTGGTCATGAGTTCGAATCTCCCACCCCAATGGTTGATGTACTCAATAAAAAATAAAAATAATCAATTAGTTTTTAAAAATTCCAAATTTATCAAGGACACAAAATTAAAGTTAAAAGACTTATTAGATATAAAATAAAAAATTTGAAGATTTATTAGATATTTTTTGCCCATCATTTTAACTTCTCCCAACTTTCCATAAAGAAAATCTCTAGTAGTACAAGAATATCATTCTCTATGTTCACACTGTGTTTTCAAAAGTTAGACTAATCTCACATGATTCATGATGGAACTTACATAGAAAAATACTTGGGTGCAGCCTCAGTTACACTCATTTAATATGCAACCCAATGGAGAGTCACAAAAAAATAATTATTTTTTTTAACTTTTTATAAAAAAAATTTGTAGCTGGAAAATAGATTGCAGTGTATCCTGCAACTCATTCTAAAAAAGATCATGACATGACAAACAATCATTTTTCTTCCTCCCCTACTCTACTACTCAAGTTTAAAATGTCGTTATCGATGGAAATATTGAAGTCTCAATTTCATGAAATGTCGATGGAAATATTGAAAAATTTTCGATGTCGATGGATATTTGTAAAAAAAAATTAAAAGTAAAAAATTTTAAAAATATATTTAAATTAGAAAATAAACCTTCTACATTTTTTAAATGAGTTAAAATACTTGTTATAATATTATATTCACGTTAGGTTACATTTTATTGTAATTTTCTTTAATATTTCATCACTTTTGTATGATATAATGGAAATATCGATTATCGCTCGTGTCGATGTCAAACCCATGGACATGTCTACGGAAATTTAATATCATGCTACTACTAGATATTTTTCTCAATAAAGAGAGGTCTTCAACAATAATAATTCTAAGCCATAACTTACTGCAGATTTCAAAGATGGTGATTGCCAATGGTGAAGATATTCTTTCATTTCCCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTTAATTTTCATTATTCTCAAACACATCAATTCTCCATCTTCAAAGAAACTCCCACTTCCTCCAGGTCCAAGGCCATGGCCAATTTTAGGAAATCTCCTTCACATTGGCCAAAATGCTCACATTTCTATCACTCACTTTGCTCAAATCTACGGCCCTCTAATATCCCTCAACCTTGGAGCTCAGCGCCTCGTCGTTGCTTCTTCTCCTGCTGCCGCCGCTGCCATTCTCAAGACTCAAGATCGCCTTCTCTCTGCTCGATACATTTTCCAAATGACTCCTGACAGAGCTTTCCATGACGATTCCTCTCTTGTTTTCTCTCCTCACTGTAACGATCGTTGGAAGAGTTTGAGGTCCATTTGCAGAGCCAATTTGTTTACAGCCAGAGCGATAGAATCCCAAGCCAATTTCAGACGAAGGAAGATGAATGACATGGTGGACTTCTTGCACTCAAAACAGGGGTCCGTTGTCGAGATTAAGGATTTTGTGTTCACCACCGTTTTCAACACTCTTTCTAACTTCATCTTCTCGAGGGATCTTCTTGGGTTCGTTGGCGATGGATTCAATGGGATTAAAGCGCCTTTCAAGAAGATGATGAATTTGGGTTTGACCCCAAATTTAGCTGACTTCTACCCTTTGCTCAGAAGCTTTGATCTTCAGGGTCTTCGAAAGAAAGCTGCCATCTACCAGGAGCAAGTCCTTTCTTCGTGGGAGATTCTCATTCAAGAAAGAAGAAAAATCCATTCTCAAAATGGCTCTGCTGCAGATCGTGACTTCTTGGATGTCTTGATCGAAAGTGGATTCACTGATCAACAAATTTATTACTTGATTGTTGTATGTGTGTAAAGAATCTCCTATTAAATTTAACCATAACCTCAAAAATTTGAGTTGATTAAGAGTCTTAAATGAATTGCAGGAGGTGTTATCAGCAGGGACAGATACAACTACGACGACGGTGGAGTGGACAATGGCAGAGTTGTTGAAAAACAGAGACATAATGAACAGAGTGCAAGAGGAGATGAAAAGGGTAATAAATAATGGAAGCGCCATTATTGAAGAATCAGAGATCTCTGAGCTTCATTATCTATGGAGATGTGTGAAAGAAACTCTAAGGCTTCATCCACCGGCGCCATTTCTTCTTCCACGAGTAGCTCCAGAAGATTGTGAAGTAATGGGATATTCCATTCCAAAAGATACTCTGATTTTTGTGAATGTTTGGGGGATCGGACGAGACCCATTAGTTTGGGAAGATTCTGAATCGTTCAATCCAGAAAGATTCGTTGGTTGCGATGTGGATTTTAAAGGTTATGATTATCGATATCTACCATTTGGAGGAGGAAGAAGAATTTGTCCTGGACTTCCCATGGCGGCTCTGCAAGTTCCTTTGATCTTAGCTACGTTAATTCATAACTTTGAATGGTCCCTTCCTAATGGTGAGGATTTATCGAAGCTGGATTTTAATGGAAGGCTTGGTGTAACATTGAAGAAGGAAGAACCTCTCAAACTTATTCCTAAAAAGAAGATTATGTGA

mRNA sequence

ATGAGTTCAAGGTCTTCAACAATAATAATTCTAAGCCATAACTTACTGCAGATTTCAAAGATGGTGATTGCCAATGGTGAAGATATTCTTTCATTTCCCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTTAATTTTCATTATTCTCAAACACATCAATTCTCCATCTTCAAAGAAACTCCCACTTCCTCCAGGTCCAAGGCCATGGCCAATTTTAGGAAATCTCCTTCACATTGGCCAAAATGCTCACATTTCTATCACTCACTTTGCTCAAATCTACGGCCCTCTAATATCCCTCAACCTTGGAGCTCAGCGCCTCGTCGTTGCTTCTTCTCCTGCTGCCGCCGCTGCCATTCTCAAGACTCAAGATCGCCTTCTCTCTGCTCGATACATTTTCCAAATGACTCCTGACAGAGCTTTCCATGACGATTCCTCTCTTGTTTTCTCTCCTCACTGTAACGATCGTTGGAAGAGTTTGAGGTCCATTTGCAGAGCCAATTTGTTTACAGCCAGAGCGATAGAATCCCAAGCCAATTTCAGACGAAGGAAGATGAATGACATGGTGGACTTCTTGCACTCAAAACAGGGGTCCGTTGTCGAGATTAAGGATTTTGTGTTCACCACCGTTTTCAACACTCTTTCTAACTTCATCTTCTCGAGGGATCTTCTTGGGTTCGTTGGCGATGGATTCAATGGGATTAAAGCGCCTTTCAAGAAGATGATGAATTTGGGTTTGACCCCAAATTTAGCTGACTTCTACCCTTTGCTCAGAAGCTTTGATCTTCAGGGTCTTCGAAAGAAAGCTGCCATCTACCAGGAGCAAGTCCTTTCTTCGTGGGAGATTCTCATTCAAGAAAGAAGAAAAATCCATTCTCAAAATGGCTCTGCTGCAGATCGTGACTTCTTGGATGTCTTGATCGAAAGTGGATTCACTGATCAACAAATTTATTACTTGATTGTTGAGGTGTTATCAGCAGGGACAGATACAACTACGACGACGGTGGAGTGGACAATGGCAGAGTTGTTGAAAAACAGAGACATAATGAACAGAGTGCAAGAGGAGATGAAAAGGGTAATAAATAATGGAAGCGCCATTATTGAAGAATCAGAGATCTCTGAGCTTCATTATCTATGGAGATGTGTGAAAGAAACTCTAAGGCTTCATCCACCGGCGCCATTTCTTCTTCCACGAGTAGCTCCAGAAGATTGTGAAGTAATGGGATATTCCATTCCAAAAGATACTCTGATTTTTGTGAATGTTTGGGGGATCGGACGAGACCCATTAGTTTGGGAAGATTCTGAATCGTTCAATCCAGAAAGATTCGTTGGTTGCGATGTGGATTTTAAAGGTTATGATTATCGATATCTACCATTTGGAGGAGGAAGAAGAATTTGTCCTGGACTTCCCATGGCGGCTCTGCAAGTTCCTTTGATCTTAGCTACGTTAATTCATAACTTTGAATGGTCCCTTCCTAATGGTGAGGATTTATCGAAGCTGGATTTTAATGGAAGGCTTGGTGTAACATTGAAGAAGGAAGAACCTCTCAAACTTATTCCTAAAAAGAAGATTATGTGA

Coding sequence (CDS)

ATGAGTTCAAGGTCTTCAACAATAATAATTCTAAGCCATAACTTACTGCAGATTTCAAAGATGGTGATTGCCAATGGTGAAGATATTCTTTCATTTCCCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTTAATTTTCATTATTCTCAAACACATCAATTCTCCATCTTCAAAGAAACTCCCACTTCCTCCAGGTCCAAGGCCATGGCCAATTTTAGGAAATCTCCTTCACATTGGCCAAAATGCTCACATTTCTATCACTCACTTTGCTCAAATCTACGGCCCTCTAATATCCCTCAACCTTGGAGCTCAGCGCCTCGTCGTTGCTTCTTCTCCTGCTGCCGCCGCTGCCATTCTCAAGACTCAAGATCGCCTTCTCTCTGCTCGATACATTTTCCAAATGACTCCTGACAGAGCTTTCCATGACGATTCCTCTCTTGTTTTCTCTCCTCACTGTAACGATCGTTGGAAGAGTTTGAGGTCCATTTGCAGAGCCAATTTGTTTACAGCCAGAGCGATAGAATCCCAAGCCAATTTCAGACGAAGGAAGATGAATGACATGGTGGACTTCTTGCACTCAAAACAGGGGTCCGTTGTCGAGATTAAGGATTTTGTGTTCACCACCGTTTTCAACACTCTTTCTAACTTCATCTTCTCGAGGGATCTTCTTGGGTTCGTTGGCGATGGATTCAATGGGATTAAAGCGCCTTTCAAGAAGATGATGAATTTGGGTTTGACCCCAAATTTAGCTGACTTCTACCCTTTGCTCAGAAGCTTTGATCTTCAGGGTCTTCGAAAGAAAGCTGCCATCTACCAGGAGCAAGTCCTTTCTTCGTGGGAGATTCTCATTCAAGAAAGAAGAAAAATCCATTCTCAAAATGGCTCTGCTGCAGATCGTGACTTCTTGGATGTCTTGATCGAAAGTGGATTCACTGATCAACAAATTTATTACTTGATTGTTGAGGTGTTATCAGCAGGGACAGATACAACTACGACGACGGTGGAGTGGACAATGGCAGAGTTGTTGAAAAACAGAGACATAATGAACAGAGTGCAAGAGGAGATGAAAAGGGTAATAAATAATGGAAGCGCCATTATTGAAGAATCAGAGATCTCTGAGCTTCATTATCTATGGAGATGTGTGAAAGAAACTCTAAGGCTTCATCCACCGGCGCCATTTCTTCTTCCACGAGTAGCTCCAGAAGATTGTGAAGTAATGGGATATTCCATTCCAAAAGATACTCTGATTTTTGTGAATGTTTGGGGGATCGGACGAGACCCATTAGTTTGGGAAGATTCTGAATCGTTCAATCCAGAAAGATTCGTTGGTTGCGATGTGGATTTTAAAGGTTATGATTATCGATATCTACCATTTGGAGGAGGAAGAAGAATTTGTCCTGGACTTCCCATGGCGGCTCTGCAAGTTCCTTTGATCTTAGCTACGTTAATTCATAACTTTGAATGGTCCCTTCCTAATGGTGAGGATTTATCGAAGCTGGATTTTAATGGAAGGCTTGGTGTAACATTGAAGAAGGAAGAACCTCTCAAACTTATTCCTAAAAAGAAGATTATGTGA

Protein sequence

MSSRSSTIIILSHNLLQISKMVIANGEDILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISITHFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLVFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKKAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLGVTLKKEEPLKLIPKKKIM

Homology

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BLAST of Tan0002865 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 7.2e-119
Identity = 228/497 (45.88%), Postives = 316/497 (63.58%), Query Frame = 0


Query: 29  ILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISIT 88
           I+SF FLL L        F IL+      S+   LPPGPRP PI+GNLL +G   H    
Sbjct: 8   IVSFSFLLFL--------FFILR-----DSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFA 67

Query: 89  HFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLV 148
             AQ YG L SL LG+Q +VVASSPAAAA ILKT D++LS RY+FQ    +  H ++S+V
Sbjct: 68  KLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENSIV 127

Query: 149 FSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFT 208
           +S  CND WK LR +CR  LFT + IESQ+  R  K  +MV FL  K+G VV+I + VF 
Sbjct: 128 WS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFG 187

Query: 209 TVFNTLSNFIFSRDLLGFVG--DGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLR 268
           T+ N   N IFS+D+        G   +K    K++++G + N AD++P++   DL G R
Sbjct: 188 TLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQR 247

Query: 269 KKAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSA 328
           +  A   +Q+   W ++++ERR      GS +  DF+DVL+ +G  DQ+I  L++E+  A
Sbjct: 248 RAVAEVLQQIYDVWGVMLKERR---GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGA 307

Query: 329 GTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETL 388
           GT+T+ +T+EW + EL K   ++++++ E+  V+  G   ++ES++  L YL   VKETL
Sbjct: 308 GTETSASTIEWAITELTKKPLVVSKIRLELVNVV--GDNTVKESDLPHLPYLQAFVKETL 367

Query: 389 RLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCD 448
           RLHPP P LLPR A E C VM Y+IPK+  I VN W IGRDP  W+D  +F PERF+  D
Sbjct: 368 RLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSD 427

Query: 449 VDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRL 508
           VD+KG D+  +PFGGGRRICPGLP+A+    LI+ATL+ NFEWSLP G   S+L  + + 
Sbjct: 428 VDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKF 484

Query: 509 GVTLKKEEPLKLIPKKK 524
           G+TL+K+ PL ++ K +
Sbjct: 488 GLTLQKDPPLLIVLKAR 484

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BLAST of Tan0002865 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.7e-118
Identity = 209/464 (45.04%), Postives = 302/464 (65.09%), Query Frame = 0


Query: 63  LPPGPRPWPILGNLLHIGQNAHISITHFAQIYGPLISLNLGAQRLVVASSPAAAAAILKT 122
           LPPGP+PWPI+GNLL +G+  H      AQ YG + +L +G + +VVAS+ +AA+ ILKT
Sbjct: 28  LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 87

Query: 123 QDRLLSARYIFQMTPDRAFHDDSSLVFSPHCNDRWKSLRSICRANLFTARAIESQANFRR 182
            DR+LSARY+FQ    +  H ++S+V+S  C + WK+LR +CR  LFT + IESQA+ R 
Sbjct: 88  HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 147

Query: 183 RKMNDMVDFLHSKQGSVVEIKDFVFTTVFNTLSNFIFSRDL--LGFVGDGFNGIKAPFKK 242
           +K  +MV++L  KQG  V+I + +F T+ N   N IFS+++  LG    G +  K    +
Sbjct: 148 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWR 207

Query: 243 MMNLGLTPNLADFYPLLRSFDLQGLRKKAAIYQEQVLSSWEILIQERRKIHSQNGSAADR 302
           M+ LG + N AD++P+L  FDL G RK+ A   + + + W  ++QER+     +G  +  
Sbjct: 208 MLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKN 267

Query: 303 DFLDVLIESGFTDQQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVI 362
           DF+DV ++SG  D QI  L++E+  AGT+T+ +T+EW M EL KN  I  +++ E++ V+
Sbjct: 268 DFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVV 327

Query: 363 NNGSAIIEESEISELHYLWRCVKETLRLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVN 422
             G   ++ES+   L YL   VKETLRLHPP P LLPR A E C ++ Y+IPKD  I VN
Sbjct: 328 --GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVN 387

Query: 423 VWGIGRDPLVWEDSESFNPERFVGCDVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLIL 482
            WGIGRDP  W D  +F+PERF+   VDF+G D+  +PFG GRRICPGLP+A   + L++
Sbjct: 388 AWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLV 447

Query: 483 ATLIHNFEWSLPNGEDLSKLDFNGRLGVTLKKEEPLKLIPKKKI 525
           AT + N +W LPNG  +  L    + G+TL+KE PL ++PK ++
Sbjct: 448 ATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSRV 487

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BLAST of Tan0002865 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 4.0e-117
Identity = 213/490 (43.47%), Postives = 314/490 (64.08%), Query Frame = 0


Query: 37  LLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISITHFAQIYGP 96
           ++ + L+ +I   + ++   SS    LPPGP+PWPI+GNLL +G+  H      AQ YG 
Sbjct: 3   VVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGD 62

Query: 97  LISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLVFSPHCNDR 156
           + +L +G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H ++S+V+S  C + 
Sbjct: 63  IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTET 122

Query: 157 WKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFTTVFNTLSN 216
           WK+LR +CR  LFT + IESQA+ R +K  +MV++L  KQG  V+I + +F T+ N   N
Sbjct: 123 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 182

Query: 217 FIFSRDL--LGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKKAAIYQE 276
            IFS+++  LG    G +  K    +M+ LG + N AD++P+L  FDL G RK+ A   +
Sbjct: 183 LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 242

Query: 277 QVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAGTDTTTTT 336
            + + W  ++QER+     +G  +  DF+DV ++SG  D QI  L++E+  AGT+T+ +T
Sbjct: 243 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 302

Query: 337 VEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRLHPPAPF 396
           +EW M EL KN  I  +++ E++ V+  G   ++ES+   L YL   VKETLRLHPP P 
Sbjct: 303 IEWAMTELTKNPKITAKLRSELQTVV--GERSVKESDFPNLPYLEATVKETLRLHPPTPL 362

Query: 397 LLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDVDFKGYDY 456
           LLPR A E C ++ Y+IPKD  I VN WGIGRDP  W D  +F+PERF+   VDF+G D+
Sbjct: 363 LLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDF 422

Query: 457 RYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLGVTLKKEE 516
             +PFG GRRICPGLP+A   + L++AT + N +W LPNG  +  L    + G+TL+KE 
Sbjct: 423 SLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEP 482

Query: 517 PLKLIPKKKI 525
           PL ++PK ++
Sbjct: 483 PLFIVPKSRV 488

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BLAST of Tan0002865 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 4.1e-114
Identity = 213/489 (43.56%), Postives = 313/489 (64.01%), Query Frame = 0


Query: 37  LLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISITHFAQIYGP 96
           L+ +++   +++I +  +SP      LPPGP+PWPI+GNLL +G+  H      A+ YG 
Sbjct: 2   LVAVVITTFLYLIFRD-SSPKG----LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGD 61

Query: 97  LISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLVFSPHCNDR 156
           L SL LG++ +VVAS+P AA+ ILKT DR+LS RY+FQ    +  H ++S+V+S  CN+ 
Sbjct: 62  LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNET 121

Query: 157 WKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFTTVFNTLSN 216
           WK LR +CR  LFT + IESQA  R  K  +MV++L    G+ V+I + VF T+ N   N
Sbjct: 122 WKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 181

Query: 217 FIFSRDL--LGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKKAAIYQE 276
            IFS+++  LG    G   +K    +M+ LG + N AD++P L  FDL G  K  A   +
Sbjct: 182 LIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQ 241

Query: 277 QVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAGTDTTTTT 336
            + S W  +++E RKI  Q+ ++   DF+++L++SG  DQQI  L++E+  AGT+T+ +T
Sbjct: 242 GIYSVWGAMLKE-RKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAST 301

Query: 337 VEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRLHPPAPF 396
           +EW ++EL KN  +   ++ E+  V+  G   ++ES+I  + YL   VKETLRLHP  P 
Sbjct: 302 IEWALSELTKNPQVTANMRLELLSVV--GKRPVKESDIPNMPYLQAFVKETLRLHPATPL 361

Query: 397 LLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDVDFKGYDY 456
           LLPR A E C+V+ Y+IPK+  I VN WGIGRDP  W D   F+PERF+   +DFKG D+
Sbjct: 362 LLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDF 421

Query: 457 RYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLGVTLKKEE 516
             +PFG GRRICPG+P+A   + LI+++L+ NF+W  P G D S+L    + G+TL+KE 
Sbjct: 422 ELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEP 480

Query: 517 PLKLIPKKK 524
           PL ++PK +
Sbjct: 482 PLYIVPKTR 480

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BLAST of Tan0002865 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 9.8e-108
Identity = 210/492 (42.68%), Postives = 314/492 (63.82%), Query Frame = 0


Query: 38  LLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNA----HISITHFAQI 97
           L + L  LI +IL  I     K   LPPGP PWP++GNL  +  N     HI++ + A+ 
Sbjct: 3   LQIALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMART 62

Query: 98  YGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLVFSPHC 157
           +GP++ L LG Q  V+AS+  AA  ILKT DR+ SAR+I +M+     H   SLV+S  C
Sbjct: 63  HGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS-DC 122

Query: 158 NDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFTTVFNT 217
            D WK LR I R  +F+ + +++Q++ R +K+ +++DFL SK+G VV+I  FVF T+ N 
Sbjct: 123 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 182

Query: 218 LSNFIFSRDLLGFVGDGFN--GIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKKAAI 277
           L N +FS+D+  + GD  +  GI+   ++M+ +G  PN+A+FYP L   DLQGL+KK   
Sbjct: 183 LGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDE 242

Query: 278 YQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAGTDTT 337
              +V+  WE  ++ER+     N +   +D LDVL+ + F D QI  L +E    G++T+
Sbjct: 243 RFIRVMKMWEGTVKERK----ANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETS 302

Query: 338 TTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRLHPP 397
           + T+EW +AEL+K+   M +V++E+  V+  G++ I+ES++ +L YL  C+KE +RLHP 
Sbjct: 303 SATIEWVIAELIKSPKEMAKVRKELNEVV--GTSTIKESDLPQLPYLQACIKEAMRLHPA 362

Query: 398 APFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDVDFKG 457
           APFLLPR A E CEVMGY+IPK++ + VN + IGRDP  W+D  +F PERF+  DVDF G
Sbjct: 363 APFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHG 422

Query: 458 YDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLGVTLK 517
             Y+++PFG GRR C G+P+A   +PLI+ +L+HN+++ LP G     L  N  L +TL 
Sbjct: 423 AHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLA 482

Query: 518 KEEPLKLIPKKK 524
            +  L ++PK +
Sbjct: 483 IDPSLCVVPKAR 485

[Close]

BLAST of Tan0002865 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 796.6 bits (2056), Expect = 1.3e-226
Identity = 400/496 (80.65%), Postives = 440/496 (88.71%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F    LLLLLPLIFIIL+HI+S SSK+ PLPPGP+PWPILGNLL IGQNAHIS+
Sbjct: 2   EIASFSF----LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+ IYGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDR  HD+ SL
Sbjct: 62  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           +FSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMN+MV+FL SKQGSVVEIK+ VF
Sbjct: 122 LFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGIK PFK M++LGL PNLADFYPLLR FDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ QV SSWEILI+ERR IHSQ  SAA  DFLDVLI+SGFTD+QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQVDSSWEILIKERRTIHSQGVSAAS-DFLDVLIQSGFTDEQISYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAELLKNRDIMNRV+EE K+ IN G+  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF GCD 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRRICPGLPMAA+QVPLILATLIHNFEW LPN ED S LD NGRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLG 481

Query: 508 VTLKKEEPLKLIPKKK 524
           VTL+KEEPLKL+ K +
Sbjct: 482 VTLQKEEPLKLLSKNR 489

[Close]

BLAST of Tan0002865 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 788.1 bits (2034), Expect = 4.7e-224
Identity = 399/496 (80.44%), Postives = 437/496 (88.10%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F    LLLLLPLIF IL+HI S SSK+ PLPPGP PWPILGNLL IGQNAHIS+
Sbjct: 2   EIASFSF----LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+  YGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDR  HD+ SL
Sbjct: 62  TQFSHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           +FSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMNDMV+FL SKQGSVVEIK+ VF
Sbjct: 122 LFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGIK  FK +++LGL PNLADFYPLLRSFDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ QV SSWEILI+ERR IHS++ SAA  DFLDVLIESGFTD+QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQVDSSWEILIKERRTIHSRDVSAAS-DFLDVLIESGFTDEQISYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAELLKNRDIMNRV+EE K+ IN G+  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PKDT+IFVNVWGIGRDP +WED  SF PERF G D 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRRICPGLPMAA+QVPLILATLIHNFEW LPN ED S LD NGRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLG 481

Query: 508 VTLKKEEPLKLIPKKK 524
           VTL+KEEPLKL+ KK+
Sbjct: 482 VTLQKEEPLKLLSKKR 489

[Close]

BLAST of Tan0002865 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 777.3 bits (2006), Expect = 8.2e-221
Identity = 391/496 (78.83%), Postives = 438/496 (88.31%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F   LLLLLLPLI  ILKHI+S SSK+ PLPPGP+PWP+LGNLL IGQNAHIS+
Sbjct: 2   EIASFSF---LLLLLLPLIVAILKHISS-SSKQPPLPPGPKPWPMLGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+ IYGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDRA HD+ SL
Sbjct: 62  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           VFSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMN+MV+FL SKQGSVVEIK+FVF
Sbjct: 122 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGIK  FK +++L L PNLADFYPLLR FDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ Q+ SSWEILI+ERR IHS+  SAA  DFLDVLI+SG+T++QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQIDSSWEILIKERRTIHSRGVSAA-TDFLDVLIQSGYTNEQINYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TD  TTTVEW MAELLKNRDIMNRV+EE K+ IN G+  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TD--TTTVEWAMAELLKNRDIMNRVREETKKAINGGA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF GCD 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRR+CPGLPMAA+QVPLILATLIHNFEW LPN E+ S LD NGRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLG 481

Query: 508 VTLKKEEPLKLIPKKK 524
           +TL+KEEPLKL+PKK+
Sbjct: 482 ITLQKEEPLKLLPKKR 488

[Close]

BLAST of Tan0002865 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.5 bits (1996), Expect = 1.2e-219
Identity = 387/496 (78.02%), Postives = 433/496 (87.30%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F     LLLLPLIF +LKHI+S SSK+ PLPPGP PWPILGNLL IGQNAHIS+
Sbjct: 2   EIASFSF-----LLLLPLIFTVLKHISS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+ IYGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYI QMTPDRA HD+ SL
Sbjct: 62  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           +FSP C DRWK+LRSICRA LFTARAIESQA+ RR+KM DMV+FL SKQGSVVEIK+FVF
Sbjct: 122 LFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQGSVVEIKEFVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSN IFSRDL+ FVGDGFNGIK PFK M++LGL PNLADFYPLLRSFDLQGLRK
Sbjct: 182 ITVFNTLSNLIFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ Q+ SS EILI+ERR IHS+  SAA  DFLD+LIESGFT++QI YLI+EVL AG
Sbjct: 242 KAAIYKNQIDSSSEILIKERRTIHSRGVSAAS-DFLDILIESGFTNEQISYLILEVLLAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAEL+KNRDIMNRV+EE K+ +N G+  I+ES++SELHYLW+CVKET+R
Sbjct: 302 TDTTTTTVEWAMAELMKNRDIMNRVREETKKALNGGA--IDESKLSELHYLWKCVKETVR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF   D 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRR CPGLPMAA+QVPLILATLIHNFEW LPN ED S LD NGRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLG 481

Query: 508 VTLKKEEPLKLIPKKK 524
           +TL+KEEPLKL+PKK+
Sbjct: 482 ITLQKEEPLKLVPKKR 488

[Close]

BLAST of Tan0002865 vs. NCBI nr
Match: XP_022929108.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 772.3 bits (1993), Expect = 2.6e-219
Identity = 387/497 (77.87%), Postives = 432/497 (86.92%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F     LLLLPLIF ILKHI+S SSK+ P PPGP+PWP+LGNLL IGQNAHIS+
Sbjct: 2   EIASFSF-----LLLLPLIFAILKHISS-SSKQPPFPPGPKPWPMLGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+ IYGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDRA HD+ SL
Sbjct: 62  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           VFSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMN+MV+FL SKQGSVVEIK+FVF
Sbjct: 122 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGI+  FK +++  L PNLADFYPLLR FDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ Q+ SSWEILI+ERR IHS+ G     DFLDVLI+SGFTD+QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQIDSSWEILIKERRTIHSR-GVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAELLKNRDIMNRV+EE K+ IN  +  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PK T+I VNVWGIGRDP +WED +SF PERF  CD 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRR+CPGLPMAA+QVPLILATLIHNFEW LPN ED S LD  GRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLG 481

Query: 508 VTLKKEEPLKLIPKKKI 525
           VTL+KEEPLKL+PK++I
Sbjct: 482 VTLQKEEPLKLLPKRRI 489

[Close]

BLAST of Tan0002865 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 6.3e-227
Identity = 400/496 (80.65%), Postives = 440/496 (88.71%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F    LLLLLPLIFIIL+HI+S SSK+ PLPPGP+PWPILGNLL IGQNAHIS+
Sbjct: 2   EIASFSF----LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+ IYGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDR  HD+ SL
Sbjct: 62  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           +FSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMN+MV+FL SKQGSVVEIK+ VF
Sbjct: 122 LFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGIK PFK M++LGL PNLADFYPLLR FDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ QV SSWEILI+ERR IHSQ  SAA  DFLDVLI+SGFTD+QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQVDSSWEILIKERRTIHSQGVSAAS-DFLDVLIQSGFTDEQISYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAELLKNRDIMNRV+EE K+ IN G+  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF GCD 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRRICPGLPMAA+QVPLILATLIHNFEW LPN ED S LD NGRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLG 481

Query: 508 VTLKKEEPLKLIPKKK 524
           VTL+KEEPLKL+ K +
Sbjct: 482 VTLQKEEPLKLLSKNR 489

[Close]

BLAST of Tan0002865 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.3e-224
Identity = 399/496 (80.44%), Postives = 437/496 (88.10%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F    LLLLLPLIF IL+HI S SSK+ PLPPGP PWPILGNLL IGQNAHIS+
Sbjct: 2   EIASFSF----LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+  YGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDR  HD+ SL
Sbjct: 62  TQFSHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           +FSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMNDMV+FL SKQGSVVEIK+ VF
Sbjct: 122 LFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGIK  FK +++LGL PNLADFYPLLRSFDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ QV SSWEILI+ERR IHS++ SAA  DFLDVLIESGFTD+QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQVDSSWEILIKERRTIHSRDVSAAS-DFLDVLIESGFTDEQISYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAELLKNRDIMNRV+EE K+ IN G+  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PKDT+IFVNVWGIGRDP +WED  SF PERF G D 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRRICPGLPMAA+QVPLILATLIHNFEW LPN ED S LD NGRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLG 481

Query: 508 VTLKKEEPLKLIPKKK 524
           VTL+KEEPLKL+ KK+
Sbjct: 482 VTLQKEEPLKLLSKKR 489

[Close]

BLAST of Tan0002865 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.3e-219
Identity = 387/497 (77.87%), Postives = 432/497 (86.92%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISI 87
           +I SF F     LLLLPLIF ILKHI+S SSK+ P PPGP+PWP+LGNLL IGQNAHIS+
Sbjct: 2   EIASFSF-----LLLLPLIFAILKHISS-SSKQPPFPPGPKPWPMLGNLLQIGQNAHISM 61

Query: 88  THFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSL 147
           T F+ IYGPLISL LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDRA HD+ SL
Sbjct: 62  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 121

Query: 148 VFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVF 207
           VFSP C DRWK+LRSICRA LFTARAIESQA+ RR+KMN+MV+FL SKQGSVVEIK+FVF
Sbjct: 122 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 181

Query: 208 TTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRK 267
            TVFNTLSNFIFSRDL+GFVGDGFNGI+  FK +++  L PNLADFYPLLR FDLQGLRK
Sbjct: 182 ITVFNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRK 241

Query: 268 KAAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAG 327
           KAAIY+ Q+ SSWEILI+ERR IHS+ G     DFLDVLI+SGFTD+QI YLIVEVLSAG
Sbjct: 242 KAAIYKNQIDSSWEILIKERRTIHSR-GVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAG 301

Query: 328 TDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLR 387
           TDTTTTTVEW MAELLKNRDIMNRV+EE K+ IN  +  I+ES++SELHYLW+CVKETLR
Sbjct: 302 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDA--IDESKLSELHYLWKCVKETLR 361

Query: 388 LHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCDV 447
           LHPPAPFLLPR APE CE+MGYS+PK T+I VNVWGIGRDP +WED +SF PERF  CD 
Sbjct: 362 LHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDA 421

Query: 448 DFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLG 507
           D KG+DYR+LPFGGGRR+CPGLPMAA+QVPLILATLIHNFEW LPN ED S LD  GRLG
Sbjct: 422 DLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLG 481

Query: 508 VTLKKEEPLKLIPKKKI 525
           VTL+KEEPLKL+PK++I
Sbjct: 482 VTLQKEEPLKLLPKRRI 489

[Close]

BLAST of Tan0002865 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.4e-218
Identity = 390/501 (77.84%), Postives = 436/501 (87.03%), Query Frame = 0


Query: 29  ILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISIT 88
           I SF  LL  LLLLLP IFIILKHI SPSS KLPLPPGP PWPILGNLL IGQNAHISIT
Sbjct: 3   IPSFSILLPSLLLLLP-IFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHISIT 62

Query: 89  HFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLV 148
            FA IYGPLISL LGAQRL+VASSPAAA A+LKTQDRLLSARYIFQMTPDRA HD  SLV
Sbjct: 63  QFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLV 122

Query: 149 FSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFT 208
           FSP C D+WK+LRS+C+ NLFTA+AIESQA  R +KM ++V FL SKQGSVVE+KDFVFT
Sbjct: 123 FSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFT 182

Query: 209 TVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKK 268
           +VFNTLSN IFS DLL FVGDGF GIK PFKKMM+LGLTPNLADFYPLLR FDLQGL+ K
Sbjct: 183 SVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNK 242

Query: 269 AAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAGT 328
           A IY+ +V S W ILI+ERR+IH Q GSA++ DFLDVLI+SGF DQQI  LI+E+LSAGT
Sbjct: 243 ADIYKNEVDSLWGILIKERREIHCQQGSASN-DFLDVLIQSGFDDQQINALIIELLSAGT 302

Query: 329 DTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRL 388
           DTTTTTVEW M+E+LK++DI+N+V+EEMK+V       I+ES ISEL YLWRCVKETLRL
Sbjct: 303 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRP-IDESNISELQYLWRCVKETLRL 362

Query: 389 HPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERF-VGC-- 448
           HPPAPFLLPR+AP DCEVMGYSIPKD+++FVNVWGIGRDP VWEDS++FNP+RF VGC  
Sbjct: 363 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 422

Query: 449 -DVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNG 508
            +VDFKGYDYRYLPFGGGRRICPGLPMA +QVPLILATLIHNFEWSLPN EDLSKLD NG
Sbjct: 423 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 482

Query: 509 RLGVTLKKEEPLKLIPKKKIM 526
           RLGVTL+K E LKLIPK++++
Sbjct: 483 RLGVTLQKYETLKLIPKRRVI 499

[Close]

BLAST of Tan0002865 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 4.1e-218
Identity = 389/501 (77.64%), Postives = 436/501 (87.03%), Query Frame = 0


Query: 29  ILSFPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHISIT 88
           I SF  LL  LLLLLP IFIILKHI SPSS KLPLPPGP PWPILGNLL IGQNAHISIT
Sbjct: 3   IPSFSILLPSLLLLLP-IFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHISIT 62

Query: 89  HFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSSLV 148
            FA IYGPLISL LGAQRL+VASSPAAA A+LKTQDRLLSARYIFQMTPDRA HD  SLV
Sbjct: 63  QFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLV 122

Query: 149 FSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHSKQGSVVEIKDFVFT 208
           FSP C D+WK+LRS+C+ NLFTA+AIESQA  R +KM ++V FL SKQGSVVE+KDFVFT
Sbjct: 123 FSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFT 182

Query: 209 TVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKK 268
           +VFNTLSN IFS DL+ FVGDGF GIK PFKKMM+LGLTPNLADFYPLLR FDLQGL+ K
Sbjct: 183 SVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNK 242

Query: 269 AAIYQEQVLSSWEILIQERRKIHSQNGSAADRDFLDVLIESGFTDQQIYYLIVEVLSAGT 328
           A IY+ +V S W ILI+ERR+IH Q GSA++ DFLDVLI+SGF DQQI  LI+E+LSAGT
Sbjct: 243 ADIYKNEVDSLWGILIKERREIHCQQGSASN-DFLDVLIQSGFDDQQINALIIELLSAGT 302

Query: 329 DTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRL 388
           DTTTTTVEW M+E+LK++DI+N+V+EEMK+V       I+ES ISEL YLWRCVKETLRL
Sbjct: 303 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRP-IDESNISELQYLWRCVKETLRL 362

Query: 389 HPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERF-VGC-- 448
           HPPAPFLLPR+AP DCEVMGYSIPKD+++FVNVWGIGRDP VWEDS++FNP+RF VGC  
Sbjct: 363 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 422

Query: 449 -DVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNG 508
            +VDFKGYDYRYLPFGGGRRICPGLPMA +QVPLILATLIHNFEWSLPN EDLSKLD NG
Sbjct: 423 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 482

Query: 509 RLGVTLKKEEPLKLIPKKKIM 526
           RLGVTL+K E LKLIPK++++
Sbjct: 483 RLGVTLQKYETLKLIPKRRVI 499

[Close]

BLAST of Tan0002865 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 361.7 bits (927), Expect = 1.0e-99
Identity = 205/513 (39.96%), Postives = 294/513 (57.31%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSKK-LPLPPGPRPWPILGNLLHIGQNAHIS 87
           DI+    L  L   +L    I        SS+K +P PPGP   PI+GN+  +G+N H S
Sbjct: 2   DIIFEQALFPLFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHS 61

Query: 88  ITHFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAF----H 147
               ++ YGP++SL  G+   VV +SP AA  +L+T D++LS+R     TP  +     H
Sbjct: 62  FADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINH 121

Query: 148 DDSSLVFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFL--HSKQGSVV 207
           D  S+V+ P  + RW+ LR +    LF+ + IE+    R  K+ ++V F+   S++   V
Sbjct: 122 DKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAV 181

Query: 208 EIKDFVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSF 267
           +I    F T  N +SN +FS DL  +  +     +     +M     P+ A+F+P L   
Sbjct: 182 DISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFL 241

Query: 268 DLQGLRKKAAIYQEQVLSSW----EILIQERRKIHSQNGSAADRDFLDVLI------ESG 327
           DLQG RK      E++   +    +  + E+    + +    +RDF+DVL+      E+ 
Sbjct: 242 DLQGNRKTLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAE 301

Query: 328 FTDQQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEES 387
                I +L++++  AGTDT ++TVEW MAELL+N + M + Q E+  VI     ++EES
Sbjct: 302 LNTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQ-KGVVEES 361

Query: 388 EISELHYLWRCVKETLRLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLV 447
           +IS L YL   VKET RLHP AP L+PR A  D EV+G+ +PKDT +FVNVW IGRDP V
Sbjct: 362 DISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNV 421

Query: 448 WEDSESFNPERFVGCDVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWS 507
           WE+S  F PERF+G D+D +G DY   PFG GRRICPGLP+A   VPL+LA+L+++F+W 
Sbjct: 422 WENSSRFKPERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWK 481

Query: 508 LPNGEDLSKLDFNGRLGVTLKKEEPLKLIPKKK 524
           LPNG     LD +   G+TL K  PL  +P KK
Sbjct: 482 LPNGVGSEDLDMDETFGLTLHKTNPLHAVPVKK 508

[Close]

BLAST of Tan0002865 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 352.1 bits (902), Expect = 8.0e-97
Identity = 198/502 (39.44%), Postives = 293/502 (58.37%), Query Frame = 0


Query: 36  LLLLLLLLPLIFIILKHINS--PSSKKLPLPPGPRPWPILGNLLHIGQNAHISITHFAQI 95
           L LL   +   F+I     S   S +   LPPGP   PI+GN+  +G+N H S    A+I
Sbjct: 9   LFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKI 68

Query: 96  YGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAF-HDDSSLVFSPH 155
           YGP++SL  G    VV +SP AA  +L+T D++LS R        R F H++ S+++ P 
Sbjct: 69  YGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRK--SNDSIRCFGHEEVSVIWLPP 128

Query: 156 CNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLH--SKQGSVVEIKDFVFTTV 215
            + RW+ LR +    +F+ +  E+    R +K+ ++V F++  S++   V+I    +TTV
Sbjct: 129 SSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTV 188

Query: 216 FNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRSFDLQGLRKKAA 275
            N +SN +FS DL  +     N  +      M     P+ A+++P +   DLQG RK   
Sbjct: 189 LNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMR 248

Query: 276 IYQEQVLSSWEILIQER---RKIHSQNGSAADRDFLDVLI------ESGFTDQQIYYLIV 335
              E++   +   +  +   + + + +   ++RDFLD L+      E+   +  I +L++
Sbjct: 249 GLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLL 308

Query: 336 EVLSAGTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRC 395
           ++ +AGTDT+++T+EW MAELL+N   M + Q EM RV+   S +++ES+IS L YL   
Sbjct: 309 DMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNS-VVQESDISGLPYLQAV 368

Query: 396 VKETLRLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPER 455
           VKET RLHP AP L+PR A  D EV+G+ +PKDT + VNVW IGRDP VWE+   F PER
Sbjct: 369 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPER 428

Query: 456 FVGCDVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLD 515
           F+G D+D KG DY   PFGGGRRICPGLP+A   V L+LA+L+++F+W LPNG     LD
Sbjct: 429 FMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLD 488

Query: 516 FNGRLGVTLKKEEPLKLIPKKK 524
            +   G+TL +   L  IP KK
Sbjct: 489 MDETFGITLHRTNTLYAIPVKK 507

[Close]

BLAST of Tan0002865 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 343.2 bits (879), Expect = 3.7e-94
Identity = 201/512 (39.26%), Postives = 294/512 (57.42%), Query Frame = 0


Query: 28  DILSFPFLLLLLLLLLPLIFIILKHINSPSSK-KLPLPPGPRPWPILGNLLHIGQNAHIS 87
           DI+S   LLLL   +L    I     +   S+    LPPGP   PI+GN+  +G++ H S
Sbjct: 2   DIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRS 61

Query: 88  ITHFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAF----H 147
               ++ YGP++SL LG+   VV +SP AA  +L+T D++LSAR     +P  A     H
Sbjct: 62  FAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINH 121

Query: 148 DDSSLVFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFL--HSKQGSVV 207
            D+SLV+ P  + RW+ LR +    L + + IE+    R  K+ ++V F+   S +   V
Sbjct: 122 QDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESV 181

Query: 208 EIKDFVFTTVFNTLSNFIFSRDLLGF-VGDGFNGIKAPFKKMMNLGLTPNLADFYPLLRS 267
           +I    F T  N +SN +FS DL  +      NG++     +M+   TP+ A+++P LR 
Sbjct: 182 DISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRF 241

Query: 268 FDLQGLRKKAAIYQEQVLSSWEILIQERRKIHSQNGSAAD---RDFLDVLI-----ESGF 327
            DLQG  K   +  E+++  +   I  +    S   +  D    DF+D L+     ES  
Sbjct: 242 LDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESEL 301

Query: 328 TDQQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVINNGSAIIEESE 387
           +   I +L++++ +AGTDT+++T+EW M ELLKN   M + Q E+  VI   + I+EES+
Sbjct: 302 SISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQ-NGIVEESD 361

Query: 388 ISELHYLWRCVKETLRLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVW 447
           IS+L YL   VKET RLH P P L+PR A  D E++G+ + KDT + VNVW IGRDP VW
Sbjct: 362 ISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVW 421

Query: 448 EDSESFNPERFVGCDVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEWSL 507
           ++   F PERF+G D+D +G DY   PFG GRRICPG+P+A   V L+LA+L+++F+W L
Sbjct: 422 DNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKL 481

Query: 508 PNGEDLSKLDFNGRLGVTLKKEEPLKLIPKKK 524
           P G     LD +   G+TL K  PL  +P KK
Sbjct: 482 PKGVLSEDLDMDETFGLTLHKTNPLHAVPVKK 507

[Close]

BLAST of Tan0002865 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 336.7 bits (862), Expect = 3.5e-92
Identity = 204/514 (39.69%), Postives = 294/514 (57.20%), Query Frame = 0


Query: 28  DILS-FPFLLLLLLLLLPLIFIILKHINSPSSKKLPLPPGPRPWPILGNLLHIGQNAHIS 87
           DI+S  P  L+   +L  L+F           +    PPGP   PI+GN+  +G+N H S
Sbjct: 2   DIISGQPLFLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHS 61

Query: 88  ITHFAQIYGPLISLNLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRAFHDDSS 147
            T  ++ YGP++SL LG    VV +S  A   +LKT D++LS RYI + T     H+ S 
Sbjct: 62  FTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSV 121

Query: 148 LVFSPHCNDRWKSLRSICRANLFTARAIESQANFRRRKMNDMVDFLHS--KQGSVVEIKD 207
               P  + R++ LR +    LF+ + I++    R +K+ ++V+FL    ++   V+I  
Sbjct: 122 GWIHP-SSSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISH 181

Query: 208 FVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKAPFKKMM-----NLGLTPNLADFYPLLRS 267
             F T  N +SN +FS +L  +  D  N   + F++M+     ++G  P+LA+F+P +R 
Sbjct: 182 VSFVTALNIISNILFSVNLGSY--DSKN--SSAFQEMVIGYQESIG-NPDLANFFPFMRF 241

Query: 268 FDLQGLRKKAAIYQEQVLSSWEILIQER---RKIHSQNGSAADRDFLDVLI------ESG 327
            DLQG  KK      ++L  +      R   +   S     + +DFLDVLI      E+ 
Sbjct: 242 LDLQGNSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETE 301

Query: 328 FTDQQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNRDIMNRVQEEMKRVI-NNGSAIIEE 387
               +I +L++++  AGTDT ++TVEW MAELL N   M +VQ+E+  VI  NG    +E
Sbjct: 302 INIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGD--FQE 361

Query: 388 SEISELHYLWRCVKETLRLHPPAPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPL 447
           S+IS+L YL   VKET RLHP APFLL R A  + E++G+++ KD+ + VNVW IGRDPL
Sbjct: 362 SDISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPL 421

Query: 448 VWEDSESFNPERFVGCDVDFKGYDYRYLPFGGGRRICPGLPMAALQVPLILATLIHNFEW 507
           VWE+   F PERF+G ++D KG DY   PFG GRRICPGLP+A   V L+LA+L++ FEW
Sbjct: 422 VWENPTHFEPERFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEW 481

Query: 508 SLPNGEDLSKLDFNGRLGVTLKKEEPLKLIPKKK 524
            LPNG     LD     G+T+ K  PL  +P KK
Sbjct: 482 KLPNGVGSEDLDMEETFGLTVHKTNPLLAVPLKK 507

[Close]

BLAST of Tan0002865 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-90
Identity = 177/479 (36.95%), Postives = 275/479 (57.41%), Query Frame = 0


Query: 63  LPPGPRPWPILGNLLHI-GQNAHISITHFAQIYGPLISLNLGAQRLVVASSPAAAAAILK 122
           LPPGP PWP++GN+  + G   H S+T  ++ +GP+++L +G+   VV SS   A  I K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 123 TQDRLLSARYIFQMTPDRAFHDDSSLVFSPHCNDRWKSLRSICRANLFTARAIESQANFR 182
             D  L+ R I++        D S  + +      W+ LR +C    F  R +++ ++ R
Sbjct: 96  KHDAALAGRKIYEAMKGGKSSDGS--LITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 183 RRKMNDMVDFLH--SKQGS-VVEIKDFVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKAPF 242
            R ++ M+ F+    + G+  +++  + F   FN + N +FSRDLL    D   G +  +
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFY 215

Query: 243 K--KMMNLGLTPNLADFYPLLRSFDLQGLRKKAAIYQEQVLSSWEILIQERRKIHSQNGS 302
              K+M     PN+ADF+PLLR  D QG+R+K   + E+        I+ER ++  +  S
Sbjct: 216 HTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKS 275

Query: 303 -AADRDFLDVLIE-----------SGFTDQQIYYLIVEVLSAGTDTTTTTVEWTMAELLK 362
               +D+LDVL+E           S F+ + I  ++ E+ +AGTDTTT+T+EW +AELL 
Sbjct: 276 DEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLH 335

Query: 363 NRDIMNRVQEEMKRVINNGSAIIEESEISELHYLWRCVKETLRLHPPAPFLLPRVAPEDC 422
           N   + ++Q E++    + +  ++E ++  L YL   + ETLRLHPP PFL+P  A   C
Sbjct: 336 NPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTC 395

Query: 423 EVMG-YSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFVGCD--VDFKGYDYRYLPFGG 482
            +   Y+IPK+T + VNVW IGRDP  W D   F PERF+      DFKG DY +LPFG 
Sbjct: 396 HIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLPFGS 455

Query: 483 GRRICPGLPMAALQVPLILATLIHNFEWSLPNGEDLSKLDFNGRLGVTLKKEEPLKLIP 521
           GRR+CP LP+A+  +PL + +++ +F+W+L NG +  ++D   R+G+TLKK  PL+ IP
Sbjct: 456 GRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510

The following BLAST results are available for this feature:

BLAST of Tan0002865 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FXW4	7.2e-119	45.88	Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3...	[more]
O64899	4.7e-118	45.04	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif...	[more]
O64900	4.0e-117	43.47	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3...	[more]
Q9SP06	4.1e-114	43.56	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum...	[more]
A8CDR5	9.8e-108	42.68	Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1	[more]

BLAST of Tan0002865 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022928791.1	1.3e-226	80.65	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata]	[more]
XP_022970204.1	4.7e-224	80.44	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima]	[more]
KAG7017133.1	8.2e-221	78.83	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma...	[more]
XP_023551257.1	1.2e-219	78.02	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ...	[more]
XP_022929108.1	2.6e-219	77.87	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata]	[more]

BLAST of Tan0002865 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ELA6	6.3e-227	80.65	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O...	[more]
A0A6J1I4V7	2.3e-224	80.44	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=...	[more]
A0A6J1ER52	1.3e-219	77.87	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O...	[more]
A0A1S3CT14	2.4e-218	77.84	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656...	[more]
A0A5A7UIU3	4.1e-218	77.64	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]

BLAST of Tan0002865 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45570.1	1.0e-99	39.96	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45550.1	8.0e-97	39.44	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]
AT2G45560.1	3.7e-94	39.26	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT1G33720.1	3.5e-92	39.69	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]
AT3G52970.1	1.1e-90	36.95	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 466..477 score: 29.31 coord: 457..466 score: 60.95 coord: 380..391 score: 39.99 coord: 326..343 score: 39.99
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 64..512 e-value: 3.7E-99 score: 332.6
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 91..110 score: 33.24 coord: 466..489 score: 31.59 coord: 379..397 score: 32.62 coord: 420..444 score: 35.55 coord: 315..332 score: 27.98 coord: 335..361 score: 29.66 coord: 456..466 score: 55.82
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 48..524 e-value: 1.0E-115 score: 389.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 59..522
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 38..493 e-value: 5.8E-16 score: 56.3
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 37..521
None	No IPR available	PANTHER	PTHR47956:SF23	(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKE	coord: 37..521
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 459..468

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0002865.1	Tan0002865.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen