Tan0002747 (gene) Snake gourd v1

BLAST of Tan0002747 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.4e-72
Identity = 176/446 (39.46%), Postives = 253/446 (56.73%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASD 61
           EE+ +P YFICPISL+IMKDPVT  +GI+YDR++I  W    + V  CP+TKQPL   SD
Sbjct: 6   EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL---EKVPSCPVTKQPLPLDSD 65

Query: 62  LTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERV-RLDV 121
           LTPNH LRRLI+ W VEN   G    +I T R    K  + + +++L     E + R + 
Sbjct: 66  LTPNHMLRRLIQHWCVENETRG--VVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 125

Query: 122 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVG-----GVEEALKILSLLS 181
           L+KL+ LA++ +  NR  M E GV K ++LFVV+C  E + G     G++E+L++L L+ 
Sbjct: 126 LQKLEVLAMDGN--NRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 185

Query: 182 NYSISETRVL----DVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKL 241
             S     +L     V ++LTWVL  E     + K+Y I +L+   +   S ++  ++  
Sbjct: 186 IPSNDAKTILMENDRVMESLTWVLHQE---DFLSKAYTIVLLRNLTEYTSSHIVE-RLNP 245

Query: 242 EFFKNITNLMKEKISN-----------------------------------SALKTTITI 301
           E FK I   +K+ +++                                    A+   + I
Sbjct: 246 EIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMI 305

Query: 302 LANLCPWGRNRIKIIEAGAVFELISLELE-KPEKNISELIFNLLAQLCSMADGRAELLRH 361
           L     W RNR  +++ GAV ELI LE+    EK I+EL+  +L++LC  A+GRAE+L H
Sbjct: 306 LLETSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAH 365

Query: 362 AGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLRVGAVSKLCMMMQSDCE 402
            GGIAVV+KR+LRVS A +DRAI IL+ +SK S    V+ EM+ VG V KLC ++  DC 
Sbjct: 366 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG 425

BLAST of Tan0002747 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 4.8e-71
Identity = 162/408 (39.71%), Postives = 250/408 (61.27%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASD 61
           EE+ +PP+F+CPISL+IMKDPV  +TGI+YDR+SIE W  + K    CP+TKQ +T A D
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKK-NSCPVTKQDITDA-D 67

Query: 62  LTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDVL 121
           LTPNHTLRRLI+SW   NA  G   ++I T RP + K+ + KL+RD A++ + +V+   L
Sbjct: 68  LTPNHTLRRLIQSWCTLNASYG--VERIPTPRPPICKSEIEKLIRDSASSHENQVK--CL 127

Query: 122 KKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKIL-------SLL 181
           K+L+ +  E+ T N+  +E  GV + +   V      G +   +EAL +L       ++L
Sbjct: 128 KRLRQIVSENAT-NKRCLEAAGVPEFLANIVSNDSENGSL--TDEALNLLYHLETSETVL 187

Query: 182 SNYSISETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFF 241
            N  ++  +  ++  +LT ++   M      + YA  +LK  ++VA   + S  +K E F
Sbjct: 188 KNL-LNNKKDNNIVKSLTKIMQRGMYES---RVYATLLLKNILEVA-DPMQSMTLKPEVF 247

Query: 242 KNITNLMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEK---PEKNI 301
             +  ++ ++IS  A K  + IL N+CPWGRNR K +EAG +  +I L +++    E+  
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 302 SELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARK 361
            E+   +L  LC  A+GRAE L H   IAVV K+ILRVS   +DRA+++L  + +  A  
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 362 DVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCI 400
            +L EML++G V+KLC+++Q  C    KEKA+E+L++H+ VW +SPC+
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of Tan0002747 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 4.8e-63
Identity = 155/432 (35.88%), Postives = 247/432 (57.18%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASD 61
           +E+ +P +F+CPISL IMKDPV  +TGI+YDRESIE W  S K    CP+TKQ +T+ +D
Sbjct: 3   QEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKK-NSCPVTKQVITE-TD 62

Query: 62  LTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDVL 121
           LTPNHTLRRLI+SW   NA  G   ++I T +P + K+ + KL+++ +++   +V+   L
Sbjct: 63  LTPNHTLRRLIQSWCTLNASYG--IERIPTPKPPICKSEIEKLIKESSSSHLNQVK--CL 122

Query: 122 KKLQALAIESDTANRGRMEEVGVAKGMVLFVVRC-----------------------FWE 181
           K+L+ + +  +T N+  +E   V + +   V                            E
Sbjct: 123 KRLRQI-VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLE 182

Query: 182 GKVGGVEEAL-KILSLLSNYSISETRVLDVFD-----ALTWVLGLEMENHII-IKSYAIE 241
            +       + + LS+L +   SET +  + +      L   L   M+  I   ++YA  
Sbjct: 183 NRFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAAL 242

Query: 242 VLKKAMDVA-PSTVLSAQMKLEFFKNITNLMKEKISNSALKTTITILANLCPWGRNRIKI 301
           +LKK ++VA P  ++   ++ E F  +  ++ ++IS+ A ++ + IL   CPWGRNR K 
Sbjct: 243 LLKKLLEVADPMQII--LLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKA 302

Query: 302 IEAGAVFELISLELE---KPEKNISELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRIL 361
           +E G +  +I L ++     E+  SE+   +L  LC  A+GRAE L H   IAVVSK+IL
Sbjct: 303 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 362

Query: 362 RVSTATNDRAIQILSLISKHSARKDVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILR 400
           RVS  T++RA+++L  + +  A   +L EML++G V+KLC+++Q  C    KEKA+E+L+
Sbjct: 363 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 422

BLAST of Tan0002747 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.5e-27
Identity = 123/403 (30.52%), Postives = 199/403 (49.38%), Query Frame = 0

Query: 4   VAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASDLT 63
           + +P +F CPISL++M+DPVT  TG +YDR SIE+W +S  +   CP+T+ PL+  + L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESW-VSIGNNTTCPVTRAPLSDFT-LI 71

Query: 64  PNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERV----RLD 123
           PNHTLRRLI+ W V N   G   ++I T +   D T +R LL   +A +   V    R  
Sbjct: 72  PNHTLRRLIQEWCVANRSNG--VERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 131

Query: 124 VLKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLL----S 183
            L++L+  A +SD  NR  +      + ++  +       ++  V E+L +L +L     
Sbjct: 132 ALRRLRGFARDSD-KNRVLIAAHNATEILIKILFSETTSSEL--VSESLALLVMLPITEP 191

Query: 184 NYSISETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLK---KAMDVAPSTVLSAQMKLE 243
           N  +S +      + LT +L        +  +  IE++    K+ D+  S   S  +   
Sbjct: 192 NQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--- 251

Query: 244 FFKNITNLMKEKISN-SALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNI 303
            F+ + +L++  IS+  ALK  I  L  LC     R   I AGA   LI       ++  
Sbjct: 252 -FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD 311

Query: 304 SELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVS-TATNDRAIQILSLISKHSAR 363
           +E     +  LC   +G A    HA  + ++ K ILRVS  AT   A  +L+L +     
Sbjct: 312 TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERW 371

Query: 364 KDVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVW 394
           ++   E    G V +L +M+QS+C    K+KA+++L++  + W
Sbjct: 372 RE---EAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Tan0002747 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.8e-26
Identity = 118/406 (29.06%), Postives = 193/406 (47.54%), Query Frame = 0

Query: 4   VAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASDLT 63
           + +P +F CPISL +M DPVT +TG +YDR SI++W         CP+T+  L+  + L 
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTT--CPVTRVALSDFT-LI 71

Query: 64  PNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERV----RLD 123
           PNHTLRRLI+ W V N   G   ++I T +   D   +R LL   +A +   V    R  
Sbjct: 72  PNHTLRRLIQEWCVANRSNG--VERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 131

Query: 124 VLKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGG--VEEALKILSLLSNY 183
            +++L+ LA +S+  NR  +      + +V  +        +    V E+L +L LL   
Sbjct: 132 AIRRLRGLARDSE-KNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMT 191

Query: 184 SISETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQM--KLEFFK 243
                 V      + ++  L  ++ I I+  A  +++  +  A S  L   +      F+
Sbjct: 192 ETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 251

Query: 244 NITNLMKEKISN-SALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISEL 303
            + +L+K  IS+  ALK  I  +  LC   + R   I AGA   LI       ++  +E 
Sbjct: 252 GVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTER 311

Query: 304 IFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVS-TATNDRAIQILSLISKHSARKDV 363
               +  LC + +G A    HA  + ++ K ILRVS  AT   A  +L+L +     +D 
Sbjct: 312 GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEERCRD- 371

Query: 364 LMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCI 400
             E    G V++L +++QSDC    K KA+ +L++  + W +   +
Sbjct: 372 --EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408

BLAST of Tan0002747 vs. NCBI nr
Match: XP_038879919.1 (E3 ubiquitin-protein ligase PUB24-like [Benincasa hispida])

HSP 1 Score: 669.8 bits (1727), Expect = 1.6e-188
Identity = 340/403 (84.37%), Postives = 375/403 (93.05%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGI+YDRESIENWFL+SKD IFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTLRRLIKSWLVENA PGG  DQIL  +P LDKTRLRKLL+DL+ ASD R+R++ 
Sbjct: 61  DLTPNHTLRRLIKSWLVENAGPGGGVDQILNPKPPLDKTRLRKLLQDLSVASDNRLRIEA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           +KKL+ALAIESDTANRG MEEVGVAKGMVLFV+RCF EG+VGG+EEAL ILSLLSNYSIS
Sbjct: 121 VKKLEALAIESDTANRGSMEEVGVAKGMVLFVIRCFREGRVGGLEEALNILSLLSNYSIS 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFFKNITNL 240
           ETRV+D F++LTWVLGL+MENHIIIKSYA+EVLKKA DVAPST+L+ Q+ LE FKNITNL
Sbjct: 181 ETRVVDFFESLTWVLGLKMENHIIIKSYAVEVLKKATDVAPSTILT-QINLELFKNITNL 240

Query: 241 MKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISELIFNLLA 300
           ++EKISNS+LKTT+TIL N+CPWG+NRIK+++ G VF+LI+LELEKP KNI+ELIFNLLA
Sbjct: 241 LREKISNSSLKTTLTILTNVCPWGQNRIKLVKVGTVFDLIALELEKPAKNITELIFNLLA 300

Query: 301 QLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLRV 360
            LCS ADGRAELLRHAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDVL+EMLRV
Sbjct: 301 HLCSTADGRAELLRHAGGIAVVSKRILRVSPATTDRAIQILSLISKHSARKDVLLEMLRV 360

Query: 361 GAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           GAVSKLCMMMQS+CE YLKEKAREILRMHSNVWSNSPCIGVYV
Sbjct: 361 GAVSKLCMMMQSNCEGYLKEKAREILRMHSNVWSNSPCIGVYV 402

BLAST of Tan0002747 vs. NCBI nr
Match: XP_008450468.1 (PREDICTED: E3 ubiquitin-protein ligase PUB24-like [Cucumis melo] >KAA0040216.1 E3 ubiquitin-protein ligase PUB24-like [Cucumis melo var. makuwa] >TYK07878.1 E3 ubiquitin-protein ligase PUB24-like [Cucumis melo var. makuwa])

HSP 1 Score: 663.3 bits (1710), Expect = 1.5e-186
Identity = 337/403 (83.62%), Postives = 373/403 (92.56%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGI+YDRESIENWFL+SKD IFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTLRRLIKSW+VENA PGG  D+ LT  PLLDKTRLRKLLR++A ASDER+R+D 
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           +KKL ALAIESDTA+RGRMEEVGVAKGMVLFV+R F EG++GG+EEALKILSLLS+YSIS
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFFKNITNL 240
           E R L+ F++LTWVLGLEMENH IIKSYAIEVLKKA DVAPST+L+ Q+ LEFFKNITNL
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILT-QINLEFFKNITNL 240

Query: 241 MKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISELIFNLLA 300
            +EKISNS+LKTT+T+L N+CPWGRNR+K++E GAVF+LI LELEKPEKNI+ELIFNLLA
Sbjct: 241 FREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLA 300

Query: 301 QLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLRV 360
            LCS ADGRAEL+ HAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDVL+EMLRV
Sbjct: 301 HLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360

Query: 361 GAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           GAVSKLCM+MQS+CE +LKEKAREILRMHSNVWSNSPCIGVYV
Sbjct: 361 GAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYV 402

BLAST of Tan0002747 vs. NCBI nr
Match: XP_004139143.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66591.1 hypothetical protein Csa_007018 [Cucumis sativus])

HSP 1 Score: 654.4 bits (1687), Expect = 6.9e-184
Identity = 340/404 (84.16%), Postives = 374/404 (92.57%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSK-DVIFCPLTKQPLTKA 60
           MEE AVPPYFICPISLQIMKDPVTATTGI+YDRESI+NWFL+SK D IFCPLTKQ LTKA
Sbjct: 1   MEEAAVPPYFICPISLQIMKDPVTATTGITYDRESIQNWFLTSKDDAIFCPLTKQSLTKA 60

Query: 61  SDLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLD 120
           SDLTPNHTLRRLIKSW+VENA   G  DQILT RPLLDKT LRK+LR++ AASDER+R+D
Sbjct: 61  SDLTPNHTLRRLIKSWMVENASSAGGDDQILTPRPLLDKTCLRKILREV-AASDERLRID 120

Query: 121 VLKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSI 180
            +KKL ALAIESDTANRGRMEEVGVAKGMVLFV+R F EG+VGG+EEALKILSLLS+YSI
Sbjct: 121 SVKKLHALAIESDTANRGRMEEVGVAKGMVLFVIRRFREGRVGGLEEALKILSLLSHYSI 180

Query: 181 SETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFFKNITN 240
           SETRVLD F++LTWVLGLEMENHIIIKSYAIEVLKKA DVAPST+L+ Q+ +EFFKNITN
Sbjct: 181 SETRVLDFFESLTWVLGLEMENHIIIKSYAIEVLKKATDVAPSTILT-QINIEFFKNITN 240

Query: 241 LMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISELIFNLL 300
           L++EKISNS+LKTT+TIL N+CP GRNR+K++E GAVF+LI+LELEKPEK  +ELIFNLL
Sbjct: 241 LLREKISNSSLKTTLTILTNICPCGRNRVKLVEVGAVFDLIALELEKPEKKTTELIFNLL 300

Query: 301 AQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLR 360
           A LCS ADGRAEL+RHAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDVL+EMLR
Sbjct: 301 AHLCSTADGRAELIRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLR 360

Query: 361 VGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           VGAVSKLCMMMQS+CE YLKEKAREILRMHSNVWSNSPCIGVYV
Sbjct: 361 VGAVSKLCMMMQSNCEGYLKEKAREILRMHSNVWSNSPCIGVYV 402

BLAST of Tan0002747 vs. NCBI nr
Match: XP_022961078.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita moschata] >KAG7023800.1 E3 ubiquitin-protein ligase PUB24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-181
Identity = 334/410 (81.46%), Postives = 367/410 (89.51%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGI+YDRESIENWF++SKD IFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTL RLIKSWLVENA PG   D+IL+ RPLLDKT LRKLLRDL  ASD+ +R D 
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDL-TASDDGLRHDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           ++KL ALAIE DTA RGRM EVGVAKGMVLFV+RCF EGKVGG+EE LKILS+L +YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQ-------MKLEF 240
           ETRVLDV D+L WVLGLEMENHIIIKSYAIEVLKKA+D APSTV+S           L+F
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQF 240

Query: 241 FKNITNLMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISE 300
           FKN+TNL++EKISNSALKTT+TIL  LC WGRNRIK++E GAVF+LI+LELEKPEKNI+E
Sbjct: 241 FKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITE 300

Query: 301 LIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDV 360
           LIFNLLA LC+MADGRAEL+RHAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDV
Sbjct: 301 LIFNLLAHLCTMADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360

Query: 361 LMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           L+EMLRVGAVSKLCM+MQSDCE YLKEKAREILRMHSNVWSNSPCIGVY+
Sbjct: 361 LVEMLRVGAVSKLCMVMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYL 409

BLAST of Tan0002747 vs. NCBI nr
Match: XP_022987475.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita maxima])

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-180
Identity = 335/417 (80.34%), Postives = 365/417 (87.53%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGI+YDRESIENWFL+SKD IFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTL RLIKSWLVENA PG   D+IL+ RPLLDKT LRKLLRDL  ASD+ +R D 
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDL-TASDDGLRHDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           ++KL ALAIE DTA RGRM EVGVAKGMVLFV+RCF EGKVGG+EE LKILS+L +YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQ------------ 240
           ETRVLDV D+L WVLGLEMENHIIIKSYAIEVLKKA+D APSTV+S              
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYELRYHEFESRYQ 240

Query: 241 --MKLEFFKNITNLMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEK 300
               L+FFKN+TNL++EKISNSALKTT+TIL  LC WGRNRIK++E GAVF LI+LELEK
Sbjct: 241 GFESLQFFKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFNLIALELEK 300

Query: 301 PEKNISELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISK 360
           PEKNI+ELIFNLLA LC+ ADGRAEL+RHAGGIAVVSKRILRVS AT DRAIQILSLISK
Sbjct: 301 PEKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISK 360

Query: 361 HSARKDVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           HSARKDVL+EMLRVGAVSKLCMMMQSDCE YLKEKAREILRMHSNVWSNSPCIGVY+
Sbjct: 361 HSARKDVLVEMLRVGAVSKLCMMMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYL 416

BLAST of Tan0002747 vs. ExPASy TrEMBL
Match: A0A5A7TDP8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1933G00240 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 7.2e-187
Identity = 337/403 (83.62%), Postives = 373/403 (92.56%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGI+YDRESIENWFL+SKD IFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTLRRLIKSW+VENA PGG  D+ LT  PLLDKTRLRKLLR++A ASDER+R+D 
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           +KKL ALAIESDTA+RGRMEEVGVAKGMVLFV+R F EG++GG+EEALKILSLLS+YSIS
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFFKNITNL 240
           E R L+ F++LTWVLGLEMENH IIKSYAIEVLKKA DVAPST+L+ Q+ LEFFKNITNL
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILT-QINLEFFKNITNL 240

Query: 241 MKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISELIFNLLA 300
            +EKISNS+LKTT+T+L N+CPWGRNR+K++E GAVF+LI LELEKPEKNI+ELIFNLLA
Sbjct: 241 FREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLA 300

Query: 301 QLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLRV 360
            LCS ADGRAEL+ HAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDVL+EMLRV
Sbjct: 301 HLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360

Query: 361 GAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           GAVSKLCM+MQS+CE +LKEKAREILRMHSNVWSNSPCIGVYV
Sbjct: 361 GAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYV 402

BLAST of Tan0002747 vs. ExPASy TrEMBL
Match: A0A1S3BP96 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492061 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 7.2e-187
Identity = 337/403 (83.62%), Postives = 373/403 (92.56%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGI+YDRESIENWFL+SKD IFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTLRRLIKSW+VENA PGG  D+ LT  PLLDKTRLRKLLR++A ASDER+R+D 
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           +KKL ALAIESDTA+RGRMEEVGVAKGMVLFV+R F EG++GG+EEALKILSLLS+YSIS
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFFKNITNL 240
           E R L+ F++LTWVLGLEMENH IIKSYAIEVLKKA DVAPST+L+ Q+ LEFFKNITNL
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILT-QINLEFFKNITNL 240

Query: 241 MKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISELIFNLLA 300
            +EKISNS+LKTT+T+L N+CPWGRNR+K++E GAVF+LI LELEKPEKNI+ELIFNLLA
Sbjct: 241 FREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLA 300

Query: 301 QLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLRV 360
            LCS ADGRAEL+ HAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDVL+EMLRV
Sbjct: 301 HLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360

Query: 361 GAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           GAVSKLCM+MQS+CE +LKEKAREILRMHSNVWSNSPCIGVYV
Sbjct: 361 GAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYV 402

BLAST of Tan0002747 vs. ExPASy TrEMBL
Match: A0A0A0M309 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G633420 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 3.4e-184
Identity = 340/404 (84.16%), Postives = 374/404 (92.57%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSK-DVIFCPLTKQPLTKA 60
           MEE AVPPYFICPISLQIMKDPVTATTGI+YDRESI+NWFL+SK D IFCPLTKQ LTKA
Sbjct: 1   MEEAAVPPYFICPISLQIMKDPVTATTGITYDRESIQNWFLTSKDDAIFCPLTKQSLTKA 60

Query: 61  SDLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLD 120
           SDLTPNHTLRRLIKSW+VENA   G  DQILT RPLLDKT LRK+LR++ AASDER+R+D
Sbjct: 61  SDLTPNHTLRRLIKSWMVENASSAGGDDQILTPRPLLDKTCLRKILREV-AASDERLRID 120

Query: 121 VLKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSI 180
            +KKL ALAIESDTANRGRMEEVGVAKGMVLFV+R F EG+VGG+EEALKILSLLS+YSI
Sbjct: 121 SVKKLHALAIESDTANRGRMEEVGVAKGMVLFVIRRFREGRVGGLEEALKILSLLSHYSI 180

Query: 181 SETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFFKNITN 240
           SETRVLD F++LTWVLGLEMENHIIIKSYAIEVLKKA DVAPST+L+ Q+ +EFFKNITN
Sbjct: 181 SETRVLDFFESLTWVLGLEMENHIIIKSYAIEVLKKATDVAPSTILT-QINIEFFKNITN 240

Query: 241 LMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISELIFNLL 300
           L++EKISNS+LKTT+TIL N+CP GRNR+K++E GAVF+LI+LELEKPEK  +ELIFNLL
Sbjct: 241 LLREKISNSSLKTTLTILTNICPCGRNRVKLVEVGAVFDLIALELEKPEKKTTELIFNLL 300

Query: 301 AQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLR 360
           A LCS ADGRAEL+RHAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDVL+EMLR
Sbjct: 301 AHLCSTADGRAELIRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLR 360

Query: 361 VGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           VGAVSKLCMMMQS+CE YLKEKAREILRMHSNVWSNSPCIGVYV
Sbjct: 361 VGAVSKLCMMMQSNCEGYLKEKAREILRMHSNVWSNSPCIGVYV 402

BLAST of Tan0002747 vs. ExPASy TrEMBL
Match: A0A6J1HAU5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461696 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 5.4e-182
Identity = 334/410 (81.46%), Postives = 367/410 (89.51%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGI+YDRESIENWF++SKD IFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTL RLIKSWLVENA PG   D+IL+ RPLLDKT LRKLLRDL  ASD+ +R D 
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDL-TASDDGLRHDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           ++KL ALAIE DTA RGRM EVGVAKGMVLFV+RCF EGKVGG+EE LKILS+L +YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQ-------MKLEF 240
           ETRVLDV D+L WVLGLEMENHIIIKSYAIEVLKKA+D APSTV+S           L+F
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQF 240

Query: 241 FKNITNLMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISE 300
           FKN+TNL++EKISNSALKTT+TIL  LC WGRNRIK++E GAVF+LI+LELEKPEKNI+E
Sbjct: 241 FKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITE 300

Query: 301 LIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDV 360
           LIFNLLA LC+MADGRAEL+RHAGGIAVVSKRILRVS AT DRAIQILSLISKHSARKDV
Sbjct: 301 LIFNLLAHLCTMADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360

Query: 361 LMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           L+EMLRVGAVSKLCM+MQSDCE YLKEKAREILRMHSNVWSNSPCIGVY+
Sbjct: 361 LVEMLRVGAVSKLCMVMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYL 409

BLAST of Tan0002747 vs. ExPASy TrEMBL
Match: A0A6J1JGZ2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485014 PE=4 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.9e-181
Identity = 335/417 (80.34%), Postives = 365/417 (87.53%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGI+YDRESIENWFL+SKD IFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDV 120
           DLTPNHTL RLIKSWLVENA PG   D+IL+ RPLLDKT LRKLLRDL  ASD+ +R D 
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDL-TASDDGLRHDA 120

Query: 121 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLLSNYSIS 180
           ++KL ALAIE DTA RGRM EVGVAKGMVLFV+RCF EGKVGG+EE LKILS+L +YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 ETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQ------------ 240
           ETRVLDV D+L WVLGLEMENHIIIKSYAIEVLKKA+D APSTV+S              
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYELRYHEFESRYQ 240

Query: 241 --MKLEFFKNITNLMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEK 300
               L+FFKN+TNL++EKISNSALKTT+TIL  LC WGRNRIK++E GAVF LI+LELEK
Sbjct: 241 GFESLQFFKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFNLIALELEK 300

Query: 301 PEKNISELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISK 360
           PEKNI+ELIFNLLA LC+ ADGRAEL+RHAGGIAVVSKRILRVS AT DRAIQILSLISK
Sbjct: 301 PEKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISK 360

Query: 361 HSARKDVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCIGVYV 404
           HSARKDVL+EMLRVGAVSKLCMMMQSDCE YLKEKAREILRMHSNVWSNSPCIGVY+
Sbjct: 361 HSARKDVLVEMLRVGAVSKLCMMMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYL 416

BLAST of Tan0002747 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 273.5 bits (698), Expect = 3.1e-73
Identity = 176/446 (39.46%), Postives = 253/446 (56.73%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASD 61
           EE+ +P YFICPISL+IMKDPVT  +GI+YDR++I  W    + V  CP+TKQPL   SD
Sbjct: 20  EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL---EKVPSCPVTKQPLPLDSD 79

Query: 62  LTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERV-RLDV 121
           LTPNH LRRLI+ W VEN   G    +I T R    K  + + +++L     E + R + 
Sbjct: 80  LTPNHMLRRLIQHWCVENETRG--VVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 139

Query: 122 LKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVG-----GVEEALKILSLLS 181
           L+KL+ LA++ +  NR  M E GV K ++LFVV+C  E + G     G++E+L++L L+ 
Sbjct: 140 LQKLEVLAMDGN--NRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 199

Query: 182 NYSISETRVL----DVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKL 241
             S     +L     V ++LTWVL  E     + K+Y I +L+   +   S ++  ++  
Sbjct: 200 IPSNDAKTILMENDRVMESLTWVLHQE---DFLSKAYTIVLLRNLTEYTSSHIVE-RLNP 259

Query: 242 EFFKNITNLMKEKISN-----------------------------------SALKTTITI 301
           E FK I   +K+ +++                                    A+   + I
Sbjct: 260 EIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMI 319

Query: 302 LANLCPWGRNRIKIIEAGAVFELISLELE-KPEKNISELIFNLLAQLCSMADGRAELLRH 361
           L     W RNR  +++ GAV ELI LE+    EK I+EL+  +L++LC  A+GRAE+L H
Sbjct: 320 LLETSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAH 379

Query: 362 AGGIAVVSKRILRVSTATNDRAIQILSLISKHSARKDVLMEMLRVGAVSKLCMMMQSDCE 402
            GGIAVV+KR+LRVS A +DRAI IL+ +SK S    V+ EM+ VG V KLC ++  DC 
Sbjct: 380 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG 439

BLAST of Tan0002747 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 270.0 bits (689), Expect = 3.4e-72
Identity = 162/408 (39.71%), Postives = 250/408 (61.27%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASD 61
           EE+ +PP+F+CPISL+IMKDPV  +TGI+YDR+SIE W  + K    CP+TKQ +T A D
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKK-NSCPVTKQDITDA-D 67

Query: 62  LTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDVL 121
           LTPNHTLRRLI+SW   NA  G   ++I T RP + K+ + KL+RD A++ + +V+   L
Sbjct: 68  LTPNHTLRRLIQSWCTLNASYG--VERIPTPRPPICKSEIEKLIRDSASSHENQVK--CL 127

Query: 122 KKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKIL-------SLL 181
           K+L+ +  E+ T N+  +E  GV + +   V      G +   +EAL +L       ++L
Sbjct: 128 KRLRQIVSENAT-NKRCLEAAGVPEFLANIVSNDSENGSL--TDEALNLLYHLETSETVL 187

Query: 182 SNYSISETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQMKLEFF 241
            N  ++  +  ++  +LT ++   M      + YA  +LK  ++VA   + S  +K E F
Sbjct: 188 KNL-LNNKKDNNIVKSLTKIMQRGMYES---RVYATLLLKNILEVA-DPMQSMTLKPEVF 247

Query: 242 KNITNLMKEKISNSALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEK---PEKNI 301
             +  ++ ++IS  A K  + IL N+CPWGRNR K +EAG +  +I L +++    E+  
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 302 SELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVSTATNDRAIQILSLISKHSARK 361
            E+   +L  LC  A+GRAE L H   IAVV K+ILRVS   +DRA+++L  + +  A  
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 362 DVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCI 400
            +L EML++G V+KLC+++Q  C    KEKA+E+L++H+ VW +SPC+
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of Tan0002747 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 243.4 bits (620), Expect = 3.4e-64
Identity = 155/432 (35.88%), Postives = 247/432 (57.18%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASD 61
           +E+ +P +F+CPISL IMKDPV  +TGI+YDRESIE W  S K    CP+TKQ +T+ +D
Sbjct: 3   QEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKK-NSCPVTKQVITE-TD 62

Query: 62  LTPNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERVRLDVL 121
           LTPNHTLRRLI+SW   NA  G   ++I T +P + K+ + KL+++ +++   +V+   L
Sbjct: 63  LTPNHTLRRLIQSWCTLNASYG--IERIPTPKPPICKSEIEKLIKESSSSHLNQVK--CL 122

Query: 122 KKLQALAIESDTANRGRMEEVGVAKGMVLFVVRC-----------------------FWE 181
           K+L+ + +  +T N+  +E   V + +   V                            E
Sbjct: 123 KRLRQI-VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLE 182

Query: 182 GKVGGVEEAL-KILSLLSNYSISETRVLDVFD-----ALTWVLGLEMENHII-IKSYAIE 241
            +       + + LS+L +   SET +  + +      L   L   M+  I   ++YA  
Sbjct: 183 NRFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAAL 242

Query: 242 VLKKAMDVA-PSTVLSAQMKLEFFKNITNLMKEKISNSALKTTITILANLCPWGRNRIKI 301
           +LKK ++VA P  ++   ++ E F  +  ++ ++IS+ A ++ + IL   CPWGRNR K 
Sbjct: 243 LLKKLLEVADPMQII--LLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKA 302

Query: 302 IEAGAVFELISLELE---KPEKNISELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRIL 361
           +E G +  +I L ++     E+  SE+   +L  LC  A+GRAE L H   IAVVSK+IL
Sbjct: 303 VEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKIL 362

Query: 362 RVSTATNDRAIQILSLISKHSARKDVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILR 400
           RVS  T++RA+++L  + +  A   +L EML++G V+KLC+++Q  C    KEKA+E+L+
Sbjct: 363 RVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLK 422

BLAST of Tan0002747 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 122.9 bits (307), Expect = 6.8e-28
Identity = 123/403 (30.52%), Postives = 199/403 (49.38%), Query Frame = 0

Query: 4   VAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASDLT 63
           + +P +F CPISL++M+DPVT  TG +YDR SIE+W +S  +   CP+T+ PL+  + L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESW-VSIGNNTTCPVTRAPLSDFT-LI 71

Query: 64  PNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERV----RLD 123
           PNHTLRRLI+ W V N   G   ++I T +   D T +R LL   +A +   V    R  
Sbjct: 72  PNHTLRRLIQEWCVANRSNG--VERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 131

Query: 124 VLKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGGVEEALKILSLL----S 183
            L++L+  A +SD  NR  +      + ++  +       ++  V E+L +L +L     
Sbjct: 132 ALRRLRGFARDSD-KNRVLIAAHNATEILIKILFSETTSSEL--VSESLALLVMLPITEP 191

Query: 184 NYSISETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLK---KAMDVAPSTVLSAQMKLE 243
           N  +S +      + LT +L        +  +  IE++    K+ D+  S   S  +   
Sbjct: 192 NQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--- 251

Query: 244 FFKNITNLMKEKISN-SALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNI 303
            F+ + +L++  IS+  ALK  I  L  LC     R   I AGA   LI       ++  
Sbjct: 252 -FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD 311

Query: 304 SELIFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVS-TATNDRAIQILSLISKHSAR 363
           +E     +  LC   +G A    HA  + ++ K ILRVS  AT   A  +L+L +     
Sbjct: 312 TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERW 371

Query: 364 KDVLMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVW 394
           ++   E    G V +L +M+QS+C    K+KA+++L++  + W
Sbjct: 372 RE---EAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Tan0002747 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 121.3 bits (303), Expect = 2.0e-27
Identity = 118/406 (29.06%), Postives = 193/406 (47.54%), Query Frame = 0

Query: 4   VAVPPYFICPISLQIMKDPVTATTGISYDRESIENWFLSSKDVIFCPLTKQPLTKASDLT 63
           + +P +F CPISL +M DPVT +TG +YDR SI++W         CP+T+  L+  + L 
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTT--CPVTRVALSDFT-LI 71

Query: 64  PNHTLRRLIKSWLVENAVPGGEADQILTSRPLLDKTRLRKLLRDLAAASDERV----RLD 123
           PNHTLRRLI+ W V N   G   ++I T +   D   +R LL   +A +   V    R  
Sbjct: 72  PNHTLRRLIQEWCVANRSNG--VERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 131

Query: 124 VLKKLQALAIESDTANRGRMEEVGVAKGMVLFVVRCFWEGKVGG--VEEALKILSLLSNY 183
            +++L+ LA +S+  NR  +      + +V  +        +    V E+L +L LL   
Sbjct: 132 AIRRLRGLARDSE-KNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMT 191

Query: 184 SISETRVLDVFDALTWVLGLEMENHIIIKSYAIEVLKKAMDVAPSTVLSAQM--KLEFFK 243
                 V      + ++  L  ++ I I+  A  +++  +  A S  L   +      F+
Sbjct: 192 ETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 251

Query: 244 NITNLMKEKISN-SALKTTITILANLCPWGRNRIKIIEAGAVFELISLELEKPEKNISEL 303
            + +L+K  IS+  ALK  I  +  LC   + R   I AGA   LI       ++  +E 
Sbjct: 252 GVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTER 311

Query: 304 IFNLLAQLCSMADGRAELLRHAGGIAVVSKRILRVS-TATNDRAIQILSLISKHSARKDV 363
               +  LC + +G A    HA  + ++ K ILRVS  AT   A  +L+L +     +D 
Sbjct: 312 GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEERCRD- 371

Query: 364 LMEMLRVGAVSKLCMMMQSDCEVYLKEKAREILRMHSNVWSNSPCI 400
             E    G V++L +++QSDC    K KA+ +L++  + W +   +
Sbjct: 372 --EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408