Tan0002743 (gene) Snake gourd v1

Overview
NameTan0002743
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionflavonol synthase/flavanone 3-hydroxylase-like
LocationLG11: 11222597 .. 11224106 (+)
RNA-Seq ExpressionTan0002743
SyntenyTan0002743
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTCGGGAGAGTCCAGGACATAGCTTCCGCGGTCCTCTTCTCCGATAGTCTCCCTTCTGAGTTCATCCGCCCGGAGACCGAGCAGCCCGGCATTACCACCGTCAAAGGCCAAACCCTCGCCGTTCCAGTCATCGATTTCAGTAACCCAGATGAAGACTCTGTTCTTCGAGCCATCGACGACGCCAGTCGCAATTGGGGCATGTTTCAAATCGTCAACCACGAGATCCCAAATGACGCAATCGCCCATTTGCAGGCCGTCGGGAAGGAATTCTTCGAGCTCCCACAGGAGGAGAAAGAGGTCTATGCTAAGCCCCCTGATTCCAAATCCGTTGAAGGCTATGGAACCAAGCTCCAAAAGGAAACCGATGGAAAGAAAGGTTGGGTCGACCATTTGTTCCATATCATTTGGCCGCCTTCTGCCATTAACTACAAATTCTGGCCCCAAAAACCCGCTTCCTACAGGTAGGAGCTATCAGATGAGAAGATCTCACCAATTTCTATCCTCTATTCCTTGATTATTGAGTTTATTTATCTGTTTTTGGGGTCTGAATTCAGGGCTGCGAATGAGGAGTACAGAAAGCATTTACATGGGGTTGTCGATAAGCTCTTTAGAAGCCTCTCGCTGGGGCTGGGGCTCGAAGAACATGAACTGAAAGAGCGTGCTGGTGGAGACGATTTGGTGTATTTAATGAAAATCAACTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCACACCGATATGTCGACCATAACAATTCTTGTGCCTAATGACGTTCAAGGTCTCCAAGCCTCTAGAGATGGGCATTGGTACGATGTCAATTACATCCCCAACGCTCTCGTCATCCACATTGGCGATCAAATTGAGGTACTGTTTCAATTTCAACTCCAGAGATTTTCATTTTTCTGCTGTGGAAAGAAGGCCTAATTGGAAGTACAACTGTTTGTTTGTGTTGATTATGTGGCTGCCTGCAGATTCTGAGTAATGGGAAGTACAAGAGCGTGCTCCACAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCACCGGCGGAGTTTGAAGTCGGGCCTCATCCAAAACTGGTCGATGAAGACAATCCGCCTAAATTCAAGACGAAGAAGTACAGTGAGTATGCATATTGCAAACTAAATAAAATTCCCCAGTGACTGAAACTGCGCCGTCGTCGTTTGTTTTGTTTTGTCTTCTCCGGCCATCCGCGGCGTTCTGCAATTCAGTTAGCAGAATGGTTTCTATTAATAACGTTTTTTAAAATCACGAGTCTGAACCAATTTAGGTTGGAATTTTATTTTGGCCAAAAAGGGAAATTAATATCCCTTTTTCTCTTTCGTGGGTTAAAAATTTTAATCTGGTTAGGAGGAGATTCACCCGAAGTGAGAATGTAATTCGGTGTTTTAATAAGAGTTGTATTTTATTGTAGGTTGAAATCTCAACTATTATTAATTAATAA

mRNA sequence

ATGGAGGTCGGGAGAGTCCAGGACATAGCTTCCGCGGTCCTCTTCTCCGATAGTCTCCCTTCTGAGTTCATCCGCCCGGAGACCGAGCAGCCCGGCATTACCACCGTCAAAGGCCAAACCCTCGCCGTTCCAGTCATCGATTTCAGTAACCCAGATGAAGACTCTGTTCTTCGAGCCATCGACGACGCCAGTCGCAATTGGGGCATGTTTCAAATCGTCAACCACGAGATCCCAAATGACGCAATCGCCCATTTGCAGGCCGTCGGGAAGGAATTCTTCGAGCTCCCACAGGAGGAGAAAGAGGTCTATGCTAAGCCCCCTGATTCCAAATCCGTTGAAGGCTATGGAACCAAGCTCCAAAAGGAAACCGATGGAAAGAAAGGTTGGGTCGACCATTTGTTCCATATCATTTGGCCGCCTTCTGCCATTAACTACAAATTCTGGCCCCAAAAACCCGCTTCCTACAGGGCTGCGAATGAGGAGTACAGAAAGCATTTACATGGGGTTGTCGATAAGCTCTTTAGAAGCCTCTCGCTGGGGCTGGGGCTCGAAGAACATGAACTGAAAGAGCGTGCTGGTGGAGACGATTTGGTGTATTTAATGAAAATCAACTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCACACCGATATGTCGACCATAACAATTCTTGTGCCTAATGACGTTCAAGGTCTCCAAGCCTCTAGAGATGGGCATTGGTACGATGTCAATTACATCCCCAACGCTCTCGTCATCCACATTGGCGATCAAATTGAGATTCTGAGTAATGGGAAGTACAAGAGCGTGCTCCACAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCACCGGCGGAGTTTGAAGTCGGGCCTCATCCAAAACTGGTCGATGAAGACAATCCGCCTAAATTCAAGACGAAGAAGTACAGTGAGTATGCATATTGCAAACTAAATAAAATTCCCCAGTGACTGAAACTGCGCCGTCGTCGTTTGTTTTGTTTTGTCTTCTCCGGCCATCCGCGGCGTTCTGCAATTCAGTTAGCAGAATGGTTTCTATTAATAACGTTTTTTAAAATCACGAGTCTGAACCAATTTAGGTTGGAATTTTATTTTGGCCAAAAAGGGAAATTAATATCCCTTTTTCTCTTTCGTGGGTTAAAAATTTTAATCTGGTTAGGAGGAGATTCACCCGAAGTGAGAATGTAATTCGGTGTTTTAATAAGAGTTGTATTTTATTGTAGGTTGAAATCTCAACTATTATTAATTAATAA

Coding sequence (CDS)

ATGGAGGTCGGGAGAGTCCAGGACATAGCTTCCGCGGTCCTCTTCTCCGATAGTCTCCCTTCTGAGTTCATCCGCCCGGAGACCGAGCAGCCCGGCATTACCACCGTCAAAGGCCAAACCCTCGCCGTTCCAGTCATCGATTTCAGTAACCCAGATGAAGACTCTGTTCTTCGAGCCATCGACGACGCCAGTCGCAATTGGGGCATGTTTCAAATCGTCAACCACGAGATCCCAAATGACGCAATCGCCCATTTGCAGGCCGTCGGGAAGGAATTCTTCGAGCTCCCACAGGAGGAGAAAGAGGTCTATGCTAAGCCCCCTGATTCCAAATCCGTTGAAGGCTATGGAACCAAGCTCCAAAAGGAAACCGATGGAAAGAAAGGTTGGGTCGACCATTTGTTCCATATCATTTGGCCGCCTTCTGCCATTAACTACAAATTCTGGCCCCAAAAACCCGCTTCCTACAGGGCTGCGAATGAGGAGTACAGAAAGCATTTACATGGGGTTGTCGATAAGCTCTTTAGAAGCCTCTCGCTGGGGCTGGGGCTCGAAGAACATGAACTGAAAGAGCGTGCTGGTGGAGACGATTTGGTGTATTTAATGAAAATCAACTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCACACCGATATGTCGACCATAACAATTCTTGTGCCTAATGACGTTCAAGGTCTCCAAGCCTCTAGAGATGGGCATTGGTACGATGTCAATTACATCCCCAACGCTCTCGTCATCCACATTGGCGATCAAATTGAGATTCTGAGTAATGGGAAGTACAAGAGCGTGCTCCACAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCACCGGCGGAGTTTGAAGTCGGGCCTCATCCAAAACTGGTCGATGAAGACAATCCGCCTAAATTCAAGACGAAGAAGTACAGTGAGTATGCATATTGCAAACTAAATAAAATTCCCCAGTGA

Protein sequence

MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQKETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLGLGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQASRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEFEVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ
Homology
BLAST of Tan0002743 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.2e-146
Identity = 239/336 (71.13%), Postives = 281/336 (83.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQ IAS     D++PSE+IR E EQP  TT+ G  L VPVID  +PDE+ +++ I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFE-LPQEEKEVYAKPPDSKSVEGYGTKL 120
            DAS+ WG+FQ++NH IP++AIA LQ VGKEFFE +PQEEKE+ AK P S  +EGYGT L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 QKETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSL 180
           QKE +GKKGWVDHLFH IWPPSA+NY++WP+ P SYR ANEEY K +  VVD++F+SLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQ 240
           GLGLE HE+ E AGGD++VYL+KINYYPPCPRPDLALGV AHTDMS ITILVPN+VQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 ASRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAE 300
             +DGHWYDV YIPNAL++HIGDQ+EILSNGKYKSV HRTTVNK+KTR+SWPVFLEPP+E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FEVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
            EVGP PKL+ E NPPKFKTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

BLAST of Tan0002743 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 9.8e-146
Identity = 240/322 (74.53%), Postives = 279/322 (86.65%), Query Frame = 0

Query: 18  SLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIVNHEI 77
           ++P+EFIR E EQPGITTV G+ L VP+IDFS+PDE+ ++  I +AS NWGM+QIVNH+I
Sbjct: 16  TIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHDI 75

Query: 78  PNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQKE-TDG---KKGWVDHL 137
           P++ I+ LQAVGKEFFELPQEEKE YAKPPDS S+EGYGTKL KE ++G   KKGWVD+L
Sbjct: 76  PSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDNL 135

Query: 138 FHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLGLGLEEHELKERAG 197
           F+ IWPPS +NY+FWP+ P SYR ANEEY KHLH VV+KLFR LSLGLGLE  ELK+ AG
Sbjct: 136 FNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAAG 195

Query: 198 GDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQASRDGHWYDVNYIP 257
           GD+L YL+KINYYPPCPRPDLALGV AHTDMST+TILVPNDVQGLQA +DG WYDV YIP
Sbjct: 196 GDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYIP 255

Query: 258 NALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEFEVGPHPKLVDEDN 317
           NALVIHIGDQ+EI+SNGKY SVLHRTTVNK+KTRISWPVFLEPPA+  VGPHP+LV+  N
Sbjct: 256 NALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAVN 315

Query: 318 PPKFKTKKYSEYAYCKLNKIPQ 336
            PK+KTKKY +Y YCK+NK+PQ
Sbjct: 316 QPKYKTKKYGDYVYCKINKLPQ 337

BLAST of Tan0002743 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 4.1e-144
Identity = 238/335 (71.04%), Postives = 276/335 (82.39%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQ+IAS     D++P+E+IR E EQP I+TV G  L VPVID S+ DE  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
            +AS+ WG+FQ+VNH IPN+ I  LQ VGK FFELPQEEKE+ AKP  S+S+EGYGT+LQ
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGKKGWVDHLFH IWPPSAINY+FWP+ P +YR ANEEY K L  VVD LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           L LE +  K+ AGGDDLVYLMKINYYPPCPRPDLALGV AHTDMS IT+LVPN+V GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            +DGHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV HRTTVNKEKTR+SWPVFLEPP + 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGP PKLV+E+NP KFKTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

BLAST of Tan0002743 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 7.8e-143
Identity = 237/337 (70.33%), Postives = 275/337 (81.60%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDF--SNPDEDSVLR 60
           MEV RVQ I+      D++PSE+IR E+EQP +TT++G  L VPVID   SN  E++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIDDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTK 120
            I +ASR WG+FQ+VNH IP+DAIA LQ VGKEFFELPQ+EKEV AKP   + VEGYGTK
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LQKETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLS 180
           LQKE  GKKGWVDHLFHIIWP SA+NY FWP  P  YR ANEEY   L GVVDKLF +LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGL 240
           LG+GLE+HELK+ +GGDDL+Y++KINYYPPCPRPDLALGV AHTDMS ITILVPN+VQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QASRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPA 300
           Q  +D HWYDV YIPNAL+IHIGDQIEI+SNGKYKSV HRTTVNK+KTR+SWPVFLEPP 
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 EFEVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           E   GP  KL+ ++NP KFKTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

BLAST of Tan0002743 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 1.6e-140
Identity = 228/335 (68.06%), Postives = 274/335 (81.79%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQ IAS    + ++P+EFIRPE EQP  TT  G    +P ID  +P +D ++R+I
Sbjct: 1   MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
            +ASR WG+FQ+ NH IP+D I  LQAVGKEFFELPQEEKEVY++P D+K V+GYGTKLQ
Sbjct: 61  AEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE +GKK WVDHLFH +WPPS+INY+FWP+ P SYRA NEEY K++  VVDKLF  LSLG
Sbjct: 121 KEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LG+E   LKE AGGDD+ Y++KINYYPPCPRPDLALGV AHTD+S +T+LVPN+V GLQ 
Sbjct: 181 LGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQV 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            +D  W D  YIPNALVIHIGDQIEILSNGKYK+VLHRTTVNK+KTR+SWPVFLEPPA+ 
Sbjct: 241 FKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADT 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
            VGP P+LVD++NPPK+K KK+ +Y+YCKLNK+PQ
Sbjct: 301 VVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335

BLAST of Tan0002743 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 302/335 (90.15%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS SLP+EF+RP+TEQPGITTVKGQ LAVPVIDF NPDEDSV+RAI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGKKGWVDHLFHIIWPPSAIN+KFWPQ P SYRAANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELKE AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.4 bits (1661), Expect = 5.3e-181
Identity = 301/335 (89.85%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS SLP+EF+RP+TEQPGITTVKGQ LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGKKGWVDHLFHIIWPPSAIN+KFWPQ P SYRAANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELKE AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. NCBI nr
Match: KAG6578981.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 300/335 (89.55%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS SLP+EF+RP+TEQPGITTVKGQ LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGKKGWVDHLFHIIWPPSAIN+KFWPQ P SYRAANEEYRKHLHGVVDKLF+SLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFKSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELKE AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. NCBI nr
Match: XP_022938382.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 300/335 (89.55%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS SLP+EF+RP+TEQPGITTVKGQ LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DG+KGWVDHLFHIIWPPSAIN+KFWPQ P SYRAANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELKE AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. NCBI nr
Match: XP_022993310.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 638.6 bits (1646), Expect = 2.9e-179
Identity = 297/335 (88.66%), Postives = 315/335 (94.03%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS S+P+EF+RP+ EQPGITTVKGQ LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGKKGWVDHLFHIIWPPSAIN+KFWPQ P SYR ANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELK+ AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.7e-181
Identity = 300/335 (89.55%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS SLP+EF+RP+TEQPGITTVKGQ LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DG+KGWVDHLFHIIWPPSAIN+KFWPQ P SYRAANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELKE AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. ExPASy TrEMBL
Match: A0A6J1HHY6 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111464499 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.4e-179
Identity = 301/335 (89.85%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           ME+ +VQDIASAVL S +LP++FIR ETEQPGITTVKGQTLAVPVIDFSNPD+ SV++AI
Sbjct: 1   MELEKVQDIASAVLSSGTLPAQFIRQETEQPGITTVKGQTLAVPVIDFSNPDQHSVIQAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASR+WGMFQI+NH+IPN+AIAHLQAVGKEFFELP EEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRHWGMFQIINHDIPNEAIAHLQAVGKEFFELPTEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE  GKK WVDHLFHIIWPPSAIN+KFWPQKP SYRAANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVGGKKDWVDHLFHIIWPPSAINFKFWPQKPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLEEHELK+ +GGDDLVYLMKINYYPPCPRPDLALGV  HTDMS ITILVPNDVQGLQA
Sbjct: 181 LGLEEHELKQSSGGDDLVYLMKINYYPPCPRPDLALGVAPHTDMSVITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            +DGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEK RISWPVFLEPPAEF
Sbjct: 241 FKDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKARISWPVFLEPPAEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVDEDNPPKFKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEDNPPKFKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.4e-179
Identity = 297/335 (88.66%), Postives = 315/335 (94.03%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIAS+ LFS S+P+EF+RP+ EQPGITTVKGQ LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASRNWGMFQI+NH+IPN+AIAHLQAVGK FFELP EEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGKKGWVDHLFHIIWPPSAIN+KFWPQ P SYR ANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELK+ AGGD LVYL+KINYYPPCP PDLALGVPAHTDMSTITILVPNDVQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPP EF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD+DNPPK+KTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. ExPASy TrEMBL
Match: A0A6J1JNE8 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111486888 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.7e-178
Identity = 298/335 (88.96%), Postives = 316/335 (94.33%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           ME+ +VQDIASAVL + +LP++FIR ETEQPGITTVKGQTLAVPV+DFSNPD+ SV++AI
Sbjct: 1   MELEKVQDIASAVLAAGTLPAQFIRQETEQPGITTVKGQTLAVPVVDFSNPDQHSVIQAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
           DDASR+WGMFQI+NH IPN+AIAHLQAVGKEFFELP EEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRHWGMFQIINHNIPNEAIAHLQAVGKEFFELPTEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE  GKK WVDHLFHIIWPPSAIN+KFWPQKP SYRAANEEYRKHLHGVVDKLFRSLSLG
Sbjct: 121 KEVGGKKDWVDHLFHIIWPPSAINFKFWPQKPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLEEHELK+ +GGDDLVYLMKINYYPPCPRPDLALGV  HTDMS ITILVPNDVQGLQA
Sbjct: 181 LGLEEHELKQSSGGDDLVYLMKINYYPPCPRPDLALGVAPHTDMSVITILVPNDVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            +DGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEK RISWPVFLEPPAEF
Sbjct: 241 FKDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKARISWPVFLEPPAEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLV+EDNPPKFKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVNEDNPPKFKTKKYGEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.3e-176
Identity = 290/335 (86.57%), Postives = 314/335 (93.73%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           M+VGRVQ++AS++LFS +LPSEF+RPETEQPGITTVKGQ LAVPVIDF+NPD+DSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
            DAS NWGMFQI+NH+IPN+AI HLQAVGKEFFELP EEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           KE DGK+GWVDHLFHIIWPPS+INY FWPQ P SYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGLE HELKER GGD+LVYL+KINYYPPCPRPDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            RDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPP EF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDE+NP K+KTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of Tan0002743 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 460.7 bits (1184), Expect = 1.0e-129
Identity = 211/334 (63.17%), Postives = 259/334 (77.54%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQDI+S+ L ++++P EFIR E EQP ITT +G T A+PV+D S+PDE+SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
             AS  WG+FQ+VNH IP + I  LQ VG++FFELP  EKE  AKP DSK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           K+ +GKK WVDHLFH IWPPS +NY+FWP+ P  YR  NEEY  H+  + + L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGL+   LKE  GG+   Y+MKINYYPPCPRPDLALGVPAHTD+S IT+LVPN+V GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            +D HW+D  YIP+A+++HIGDQI  LSNG+YK+VLHRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIP 335
            VGP P+L  +DNPPKFK   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of Tan0002743 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 460.7 bits (1184), Expect = 1.0e-129
Identity = 211/334 (63.17%), Postives = 259/334 (77.54%), Query Frame = 0

Query: 1   MEVGRVQDIASAVLFSDSLPSEFIRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQDI+S+ L ++++P EFIR E EQP ITT +G T A+PV+D S+PDE+SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  DDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEEKEVYAKPPDSKSVEGYGTKLQ 120
             AS  WG+FQ+VNH IP + I  LQ VG++FFELP  EKE  AKP DSK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAANEEYRKHLHGVVDKLFRSLSLG 180
           K+ +GKK WVDHLFH IWPPS +NY+FWP+ P  YR  NEEY  H+  + + L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVPAHTDMSTITILVPNDVQGLQA 240
           LGL+   LKE  GG+   Y+MKINYYPPCPRPDLALGVPAHTD+S IT+LVPN+V GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 SRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPAEF 300
            +D HW+D  YIP+A+++HIGDQI  LSNG+YK+VLHRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEDNPPKFKTKKYSEYAYCKLNKIP 335
            VGP P+L  +DNPPKFK   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of Tan0002743 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 341.3 bits (874), Expect = 9.0e-94
Identity = 157/295 (53.22%), Postives = 202/295 (68.47%), Query Frame = 0

Query: 40  TLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFFELPQEE 99
           +L +PVID SNPDE+ V  A+  AS+ WG+FQ+VNH IP + I  L  VG EFFELP+ E
Sbjct: 11  SLDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETE 70

Query: 100 KEVYAKPPDSKSVEGYGTKLQKETDGKKGWVDHLFHIIWPPSAINYKFWPQKPASYRAAN 159
           KE  AKP DS  +EGY TK QK+ +G+  WVDHLFH IWPPS +N+KFWP+ P  Y   N
Sbjct: 71  KEAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVN 130

Query: 160 EEYRKHLHGVVDKLFRSLSLGLGLEEHELKERAGGDDLVYLMKINYYPPCPRPDLALGVP 219
           EEY  H+  + +K+   LS GLGL    LKE  GG+ + YLMKINYYPPCP P+L +G P
Sbjct: 131 EEYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAP 190

Query: 220 AHTDMSTITILVPNDVQGLQASRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRT 279
            HTD++ IT+LV N+  GLQA +D  W D  Y  + +++ IGDQ   +SNGKYKSV HR 
Sbjct: 191 DHTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRA 250

Query: 280 TVNKEKTRISWPVFLEPPAEFEVGPHPKLV-DEDNPPKFKTKKYSEYAYCKLNKI 334
            ++KEKTRISWPVF+E   +   GP P+L+  ++N PKFK   Y +Y + KL K+
Sbjct: 251 KMDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

BLAST of Tan0002743 vs. TAIR 10
Match: AT5G63600.1 (flavonol synthase 5 )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 134/292 (45.89%), Postives = 189/292 (64.73%), Query Frame = 0

Query: 34  TTVKGQTLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFF 93
           +T+ G  + VPV+D S  DED ++R +  AS  WG+FQ+VNH IP + +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  ELPQEEKEVYAKPPDSKSVEGYGTKLQKETDGKKGWVDHLFHIIWPPSAINYKFWPQKPA 153
           ELP  EKE  AK  D    EGY    +    G   W +HLFH + PPS INYK+WP+ P 
Sbjct: 84  ELPDAEKETVAKEED---FEGY---KKNYLGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRAANEEYRKHLHGVVDKLFRSLSLGLGLEEHELKERAGGDDLVYLMKINYYPPCPRPD 213
            YR   EEY KH+  + +K+   LS GLGL+     +  GGD   Y++++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSTITILVPNDVQGLQASRDGHWYDVNYIPNALVIHIGDQIEILSNGKYK 273
           L +G  AH+DM  I +L+PN+V GLQA +D  W D++YI +A+V+ IGDQ+  ++NG+ K
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMRMTNGRLK 263

Query: 274 SVLHRTTVNKEKTRISWPVFLEPPAEFEVGPHPKLVDEDNPPKFKTKKYSEY 326
           +VLHR   +K+K RISWPVF+ P A+  VGP P+   ++NPPKF+T  Y++Y
Sbjct: 264 NVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 309

BLAST of Tan0002743 vs. TAIR 10
Match: AT5G63600.2 (flavonol synthase 5 )

HSP 1 Score: 288.1 bits (736), Expect = 9.0e-78
Identity = 134/293 (45.73%), Postives = 190/293 (64.85%), Query Frame = 0

Query: 34  TTVKGQTLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIVNHEIPNDAIAHLQAVGKEFF 93
           +T+ G  + VPV+D S  DED ++R +  AS  WG+FQ+VNH IP + +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  ELPQEEKEVYAKPPDSKSVEGYGTKLQKETDGKKGWVDHLFHIIWPPSAINYKFWPQKPA 153
           ELP  EKE  AK  D    EGY    +    G   W +HLFH + PPS INYK+WP+ P 
Sbjct: 84  ELPDAEKETVAKEED---FEGY---KKNYLGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRAANEEYRKHLHGVVDKLFRSLSLGLGLEEHELKERAGGDDLVYLMKINYYPPCPRPD 213
            YR   EEY KH+  + +K+   LS GLGL+     +  GGD   Y++++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSTITILVPNDVQGLQASRDGHWYDVNYIPNALVIHIGDQIEI-LSNGKY 273
           L +G  AH+DM  I +L+PN+V GLQA +D  W D++YI +A+V+ IGDQ+ + ++NG+ 
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMVWMTNGRL 263

Query: 274 KSVLHRTTVNKEKTRISWPVFLEPPAEFEVGPHPKLVDEDNPPKFKTKKYSEY 326
           K+VLHR   +K+K RISWPVF+ P A+  VGP P+   ++NPPKF+T  Y++Y
Sbjct: 264 KNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q075121.2e-14671.13Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ... [more]
Q9XHG29.8e-14674.53Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2... [more]
Q9M5474.1e-14471.04Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian... [more]
Q7XZQ67.8e-14370.33Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS... [more]
Q9ZWQ91.6e-14068.06Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023551148.12.4e-18190.15flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
KAG7016505.15.3e-18189.85Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp... [more]
KAG6578981.11.2e-18089.55Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp... [more]
XP_022938382.11.2e-18089.55flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata][more]
XP_022993310.12.9e-17988.66flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FDW35.7e-18189.55flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HHY61.4e-17989.85flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JY721.4e-17988.66flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1JNE82.7e-17888.96flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5D3CHN53.3e-17686.57Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
AT5G08640.11.0e-12963.17flavonol synthase 1 [more]
AT5G08640.21.0e-12963.17flavonol synthase 1 [more]
AT5G63590.19.0e-9453.22flavonol synthase 3 [more]
AT5G63600.11.8e-7845.89flavonol synthase 5 [more]
AT5G63600.29.0e-7845.73flavonol synthase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 201..296
e-value: 3.6E-25
score: 88.4
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..333
e-value: 1.9E-98
score: 331.8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 43..149
e-value: 7.3E-25
score: 88.0
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..332
NoneNo IPR availablePANTHERPTHR47990:SF36OS02G0767300 PROTEINcoord: 8..332
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 16..333
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 196..296
score: 14.583344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002743.1Tan0002743.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051555 flavonol biosynthetic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0045431 flavonol synthase activity
molecular_function GO:0046872 metal ion binding