Tan0002717 (gene) Snake gourd v1

Overview


Name	Tan0002717
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	beta-amyrin 28-oxidase
Location	LG09: 54959689 .. 54963791 (-)
RNA-Seq Expression	Tan0002717
Synteny	Tan0002717

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCATTTCCTCGTCTCTCTCTTAATCCTCTTCGTCTCCCTCCTCTCCCTCACTCTCTTCATCCTCTTCTACAAGCACAAATCCTTATTCTCCTATCCAAATCTTCCTCCCGGCACCATCGGCATTCCGATCCTCGGCGAGAGCCTGGAGTTTTTATCCAGCGGCGCCGAGAAGTTCATCTTCGACCGTATGAATAAGTACAAGTCTGATATCTTCAAAACCTCCATCATTGGCAATCCGGCCGCCATTTTCTGTGGCCCTGCCTGCAACAAGTTCCTCTTCTCCAACGAGAATAAACTCGTTACTCCGTGGTGGCCGGACTCCGTCAACAAAATCTTCCCCTCTTCCACTCAGACAAACTCCAAAGAAGAAGCTAAGAAACTCAAGAAGCTCCTCCCGCAGTTCCTTAATCCGGAAGCGCTTCAGCGTTATATCGGAATCATGGACGAACTCGCTCAACGCCATTTCGCTTCCTCTTGGGAGAACAGAGAACAAGTGCTGGTGTCTCCTCTGGCTACAAGGTAATTAAAAAAAATAAAAAGTCTCTAAAAATCGGTCTTTACCATTAGTCTATTGTTAATTTTTTTTTAAAAAAACAGTCTCTATTAGCTCTTTTTCCTACCAATTTTGAAAATATGTTTACATTGTGCCTTTTATTGTATTAAAAATATTACTATTTTGTAAGTAATTTAGATAAAAATTAACTTCAAATTTATCATAGGGACCAATTTTAAGTTTTATTGAAAGTACATAAACTAAAATTGAACATTTGAAAATACAGAGACCAAAATGGAACAATTCTCAAAGTATAAGGACCAAAATGGTATTTTAACCTAAAAAAAAAGGTTCTGTCTCTTCGAAAAAGTATGCTTGCTTTTCCATCATTTCTTTTCTACAATGATTTCCATCTTTTTTGAGGTAAAATAATATAGGCCCGTTTGATAACTTTTCTGCAATGATTTCCATGTTTTTTGAGGTAAAATAATATAGGCCCGTTTGATAACTAAAAGCTAAATGGTTTACCTATTTGGATCTTAATTATTATTAGCTTCACATTCTCGATCGCGTGCCGATTGTTCGTGAGCGTGGAGGATGGAAGCCAGATGGAAAGATTATCAAGAGCATTTGAGAAAATTGGGAGAGGAGTGTTTTCGATGCCGATTGATTTACCAGGAACGAGGTTCAATGGAGCGATTAAGGCGTCGAACTTGGTAAGAAAGGAACTGCGGTCGATAGTCAGGCAGAGGAAATCCGATCTGGCAGAAGGAAAGGCTTCAGCGACACAGGACATTCTGTCGCACATGCTTGTCACGTGCGATGAGAACGGCCAGTACATGAATGAATCCGATATCGCCGATAAGATTCTTGGATTGTTGATCGGCGGCCATGATACTACCAGTGCCACCTGTACCTTCATCGTCAAGTTCCTCGCTGACCTTCCTCATATCTACCATGGCGTCTACACAGGTTCGGTATACTAACAATAACAACACAATCATGCTTTTTTTTACAAAATTTTTATAATTGAATTTCATCTCATTTAAGACGAATGACTCGAGAAATAGCTAACATTGCAGATCAAATTTGAAACAATTTTATTTATTTACTTTTATTATTGTTGTTAATTTTGCATCGAGACTTATTGATATTAGATAATATAAATTTTTTATAATTAATTGTATTTTTATATGTCTTAATACATGTTATATATTTTATATTATATAACTAAATTCTTTTAAAATAATAAAAAATAAGAAAAAACAAGAAAAACATTCCAGAACAAGAAATAAGAAATAATTATCAAACAATCTTTTGTTTCTATTTCTTGTTTTTCCAAGAATAAGAAATAGAAATAGTTATCAAACATATTCTTGTTTCTTATTCTTACAAAACGAAAAACAAGAAACAAGAAACAAGAAATAGGAAACATGAACGTTATCAAACGAGACTTAAATAATCTATTTTGCATGCAATATGGGTCTGTTTTTCAATCAACATGATTCCACCAAACAAATTATTTAATCTTCAAATTGATTAGCTAATTGCCAAAGAGTGTCTATCTCAACTAGCATATCGAATGTGTTAACAAGTACGAGATTTGTGGTTCGAATCTCCCTAACTCCTATTATATTAAATGATCAATATGAAGTCAAAGAGAGATTGAATCTAACAATCTTACTAACAAATAAGACTAAAGATTCAAAATGTTAAGTAACCTTTGACTTAAATCATTGAAAACAAAACTTTAAAATTGAAAACTACATTAAACTAGCCATAAATCCGAGAATTGACTTTTCAATCAACCTATATAAAGTTTGTAACCTCTCATGCATAAATGGAGTCCCACTTGAAGTAGCTTGAAACTGTAATTGCATAAATGAAGTTTAATTATCTTTAATAAAACATGAGTTGCACTTGAGTTCGTTAAATGTATCTTTAGTTTGTCATAAACGAAACTTGACAATATTTAATTCAACTCTATTTTGAACCTTTTCTTTAGCATATCTCAACTGACATATAATGATTACGAGGTTTGTGGTTTGAATTATCCTAACTTCTATTGTACTAAATGATCGCAAATCAAAGAGAAATTGAATCTAACAACCTTGCTAACAAACAAGACCTTTAGATCTAAAATCCAAAAATTTCAAATCTAGATGATTTTAAAGAAGCCACAGATTCTGACATCTAAGTAACTTTTGATAATTGGATTGAACAATCTTGCTAAAATAAACTAGACCTTTAGAGCTCAACCTAAAAAAGCTCAAAATGAATTTTGAAAATCAACACAAATAAGAAATCGAATTACCTTGACAATCAAATATCTCAAGGCAAGAAAACTTGTTTGAGATTCGAATCACTCCACAAGTAAGCTTCATCAAATCAAGCTTGAATACTTCTAAGCATACAACCTAATCTACATAGAGAAACATGGCCTAATTAATTCTAAAATTATTTGACAACGAACAGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGAGAGCTATTGAACTGGGAAGACATTCAGAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTCCCCCACTCCAAGGTGCCTTTAGAGAAGTCCTAAACGACTTCGTTTTCAAAGGCTTCTACATTCCCAAGGGCTGGAAGGTAATTTCTCCTCCTATATCTCATTATCATTTTTATGAAATTTTTTGTTAAATTGCATATTTGATCCTATAATTCACAGAAAGTTAGAATTTCAACTCTCATAATTAAATAATCTATGTGGTTTTATGACAATTTTTATCAAACCACATGACTAATTCTAACCTTTAAAAATTTAAAATCATAAAGACTAAATTCTAACTTTCTGTGAACTATAGAGACTAAATTTATTATTTAATTAAAAAATTTTGAAGGATAGCATTTTTAATTTTTAAATCCTTTTGCAAATATGTAATTTTCTATGACGAGCTAATTGTCAATATGGCCATTATTTTTTTTTTAAAAAAAAACAATAATGGCAAATATAACAATTTTTTAAAATGAGTAGATCGTTTATTAACTTAAAGTATACGAAATTTGCCAACTTATAATAGATATTGATTAATTATAGTATTAGCACATCACCAATTAATCTATATATTGATCAACTATAATCTTAATATTTTAGAACTATAATATATTGATCAACTATTATTGTGATGAATTACTAACTAACTTATATATTGATTGACGTATATGTGAAATTTAAAAGTGTGAACCTGATGAGTAACAAAACAAACATTGTGAAACACTGATTCAGAAAAGTTTCAGATATATTGGAGTTCAAACGCAACACACAAGAACAAAGAGTACTTCCCAGAGCCGTACAAATTGGAGCCATCAAGATTTGAGGGCAATGGGCCAGCGCCCTACACGTTTGTGCCCTTCGGCGGAGGGCCAAGAATGTGCCCTGGAAAAGAGTACGCCAGGCTCGAGATTCTCGTCTTCATCCACAATTTGGTCAAGAGATTCAAATGGTCAAACCTTCTCGACAATGAGAAAATCATTGTTGATCCAATCCCAACCCCTCAAAAAGGTCTCCCCATTTGCCTTTTTCCTCATTAA

mRNA sequence

ATGGAGCATTTCCTCGTCTCTCTCTTAATCCTCTTCGTCTCCCTCCTCTCCCTCACTCTCTTCATCCTCTTCTACAAGCACAAATCCTTATTCTCCTATCCAAATCTTCCTCCCGGCACCATCGGCATTCCGATCCTCGGCGAGAGCCTGGAGTTTTTATCCAGCGGCGCCGAGAAGTTCATCTTCGACCGTATGAATAAGTACAAGTCTGATATCTTCAAAACCTCCATCATTGGCAATCCGGCCGCCATTTTCTGTGGCCCTGCCTGCAACAAGTTCCTCTTCTCCAACGAGAATAAACTCGTTACTCCGTGGTGGCCGGACTCCGTCAACAAAATCTTCCCCTCTTCCACTCAGACAAACTCCAAAGAAGAAGCTAAGAAACTCAAGAAGCTCCTCCCGCAGTTCCTTAATCCGGAAGCGCTTCAGCGTTATATCGGAATCATGGACGAACTCGCTCAACGCCATTTCGCTTCCTCTTGGGAGAACAGAGAACAAGTGCTGGTGTCTCCTCTGGCTACAAGCTTCACATTCTCGATCGCGTGCCGATTGTTCGTGAGCGTGGAGGATGGAAGCCAGATGGAAAGATTATCAAGAGCATTTGAGAAAATTGGGAGAGGAGTGTTTTCGATGCCGATTGATTTACCAGGAACGAGGTTCAATGGAGCGATTAAGGCGTCGAACTTGGTAAGAAAGGAACTGCGGTCGATAGTCAGGCAGAGGAAATCCGATCTGGCAGAAGGAAAGGCTTCAGCGACACAGGACATTCTGTCGCACATGCTTGTCACGTGCGATGAGAACGGCCAGTACATGAATGAATCCGATATCGCCGATAAGATTCTTGGATTGTTGATCGGCGGCCATGATACTACCAGTGCCACCTGTACCTTCATCGTCAAGTTCCTCGCTGACCTTCCTCATATCTACCATGGCGTCTACACAGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGAGAGCTATTGAACTGGGAAGACATTCAGAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTCCCCCACTCCAAGGTGCCTTTAGAGAAGTCCTAAACGACTTCGTTTTCAAAGGCTTCTACATTCCCAAGGGCTGGAAGATATATTGGAGTTCAAACGCAACACACAAGAACAAAGAGTACTTCCCAGAGCCGTACAAATTGGAGCCATCAAGATTTGAGGGCAATGGGCCAGCGCCCTACACGTTTGTGCCCTTCGGCGGAGGGCCAAGAATGTGCCCTGGAAAAGAGTACGCCAGGCTCGAGATTCTCGTCTTCATCCACAATTTGGTCAAGAGATTCAAATGGTCAAACCTTCTCGACAATGAGAAAATCATTGTTGATCCAATCCCAACCCCTCAAAAAGGTCTCCCCATTTGCCTTTTTCCTCATTAA

Coding sequence (CDS)

ATGGAGCATTTCCTCGTCTCTCTCTTAATCCTCTTCGTCTCCCTCCTCTCCCTCACTCTCTTCATCCTCTTCTACAAGCACAAATCCTTATTCTCCTATCCAAATCTTCCTCCCGGCACCATCGGCATTCCGATCCTCGGCGAGAGCCTGGAGTTTTTATCCAGCGGCGCCGAGAAGTTCATCTTCGACCGTATGAATAAGTACAAGTCTGATATCTTCAAAACCTCCATCATTGGCAATCCGGCCGCCATTTTCTGTGGCCCTGCCTGCAACAAGTTCCTCTTCTCCAACGAGAATAAACTCGTTACTCCGTGGTGGCCGGACTCCGTCAACAAAATCTTCCCCTCTTCCACTCAGACAAACTCCAAAGAAGAAGCTAAGAAACTCAAGAAGCTCCTCCCGCAGTTCCTTAATCCGGAAGCGCTTCAGCGTTATATCGGAATCATGGACGAACTCGCTCAACGCCATTTCGCTTCCTCTTGGGAGAACAGAGAACAAGTGCTGGTGTCTCCTCTGGCTACAAGCTTCACATTCTCGATCGCGTGCCGATTGTTCGTGAGCGTGGAGGATGGAAGCCAGATGGAAAGATTATCAAGAGCATTTGAGAAAATTGGGAGAGGAGTGTTTTCGATGCCGATTGATTTACCAGGAACGAGGTTCAATGGAGCGATTAAGGCGTCGAACTTGGTAAGAAAGGAACTGCGGTCGATAGTCAGGCAGAGGAAATCCGATCTGGCAGAAGGAAAGGCTTCAGCGACACAGGACATTCTGTCGCACATGCTTGTCACGTGCGATGAGAACGGCCAGTACATGAATGAATCCGATATCGCCGATAAGATTCTTGGATTGTTGATCGGCGGCCATGATACTACCAGTGCCACCTGTACCTTCATCGTCAAGTTCCTCGCTGACCTTCCTCATATCTACCATGGCGTCTACACAGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGAGAGCTATTGAACTGGGAAGACATTCAGAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTCCCCCACTCCAAGGTGCCTTTAGAGAAGTCCTAAACGACTTCGTTTTCAAAGGCTTCTACATTCCCAAGGGCTGGAAGATATATTGGAGTTCAAACGCAACACACAAGAACAAAGAGTACTTCCCAGAGCCGTACAAATTGGAGCCATCAAGATTTGAGGGCAATGGGCCAGCGCCCTACACGTTTGTGCCCTTCGGCGGAGGGCCAAGAATGTGCCCTGGAAAAGAGTACGCCAGGCTCGAGATTCTCGTCTTCATCCACAATTTGGTCAAGAGATTCAAATGGTCAAACCTTCTCGACAATGAGAAAATCATTGTTGATCCAATCCCAACCCCTCAAAAAGGTCTCCCCATTTGCCTTTTTCCTCATTAA

Protein sequence

MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSGAEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPSSTQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSFTFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRSIVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCTFIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQGAFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPFGGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH

Homology

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BLAST of Tan0002717 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 8.0e-202
Identity = 329/477 (68.97%), Postives = 402/477 (84.28%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           ME F +SLL++FV  +S+ L +LFYKH+S F+ PNLPPG IG P++GESLEFLS+G    
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR++KY S++FKTS++G PAA+F G A NKFLFSNENKLV  WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           STQT+SKEEAKK++KLLPQF  PEALQRYIGIMD +AQRHFA SW+NR++V+V PLA  F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TF +ACRLF+S+ED + + +  + F  +  G+ ++PIDLPGT F+ AIKASN +RKELR+
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           I++QRK DLAEGKAS  QDILSHML+  DE+G +MNE +IADKILGLLIGGHDT SA  T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           F++K++A+LPHIY  VY EQMEIA +KAPGELLNW+D+QKM+YSWNVACEV+R+APPLQG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE + DFVF GF IPKGWK+YWS+N+THK+ E FP+P   +P+RFEG+GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFP 474
           GGGPRMCPGKEYARLEILVF+HN+VKRFKW  LL +EKIIVDP+P P KGLP+ L P
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHP 477

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BLAST of Tan0002717 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 703.4 bits (1814), Expect = 1.8e-201
Identity = 329/478 (68.83%), Postives = 401/478 (83.89%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           ME F +SLL++ V  +S+ L++L YKH+S F+ PNLPPG IG P++GESLEFLS+G    
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR++KY S++FKTS++G PAA+F G A NKFLFSNENKLV  WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           STQT+SKEEAKK++KLLPQFL PEALQRY GIMD +AQRHFA SW+NR++V+V PLA  F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TF +ACRLF+S+ED + + +  + F  +  G+ ++PIDLPGT F+ AIKASN +RKELR+
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           I++QRK DLAE KAS TQDILSHML+  DE+G +MNE  IADKILGLLIGGHDT S+  T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           F+VK++A+LPHIY  VY EQMEIA +KAPGELLNW+D+QKM+YSWNVACEV+R+APPLQG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE + DFVF GF IPKGWK+YWS+N+THK+ E FP+P K +P+RFEG GPAPYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYARLEIL+F+HNLVKRFKW  LL +EKIIVDP+P P KGLP+ L PH
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPH 478

[Close]

BLAST of Tan0002717 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 6.4e-199
Identity = 329/479 (68.68%), Postives = 393/479 (82.05%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKS-LFSYPNLPPGTIGIPILGESLEFLSSG--- 60
           M+ F V LL LFV  +SL+ + LFYK KS   S   LPPG  G P++GES EFLS+G   
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 61  -AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFP 120
             EKFIFDR+ KY S++FKTS+ G PAA FCG ACNKFLFSNENKLV  WWPDSVNK+FP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 121 SSTQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATS 180
           SSTQT+SKEEA K++KLLP FL PEALQRY+G+MD++A +HF S WEN+++VLV PLA +
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

Query: 181 FTFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELR 240
           +TF IAC++FVSVE+ +Q+ +L   F  I  G+ S+PIDLPGT FN AIK+S +VR +L 
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240

Query: 241 SIVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATC 300
            I++QRK+DL EGKAS TQDILSHML+T DENG++M E DIADKILGLLIGGHDT S+ C
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300

Query: 301 TFIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQ 360
           TF+VKFLA+LP IY GVY EQMEIAK+K  GELLNWEDIQKMKYSWNVACEVLR+APPLQ
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360

Query: 361 GAFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVP 420
           GAFRE L DF + GF IPKGWK+YWS+N+TH+N E FPEP K +PSRFEG GP PY+FVP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420

Query: 421 FGGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           FGGGPRMCPGKEYARLEILVF+H++VKRFKW  ++ +EKI+V+P+P P KGLP+ LFPH
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPH 479

[Close]

BLAST of Tan0002717 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 3.9e-196
Identity = 328/480 (68.33%), Postives = 388/480 (80.83%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHK--SLFSYPNLPPGTIGIPILGESLEFLSSG-- 60
           ME F V LL LFV  +SL+   LFYK K  S   +P LPPG  G PI+GES EFLS+G  
Sbjct: 1   MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFP-LPPGKTGWPIIGESYEFLSTGWK 60

Query: 61  --AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIF 120
              EKFIFDRM KY S++FKTSI G PAA+FCG ACNKFLFSNENKLV  WWPDSVNK+F
Sbjct: 61  GYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVF 120

Query: 121 PSSTQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLAT 180
           PSSTQT+SKEEA K++K+LP F  PEALQRYIG+MD++A  HF S WEN+ +V+V PLA 
Sbjct: 121 PSSTQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAK 180

Query: 181 SFTFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKEL 240
           S+TF IAC++FVSVE+ +Q+  L   F  I  G+ S+PIDLPGT FN AIK+S +VR++L
Sbjct: 181 SYTFWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKL 240

Query: 241 RSIVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSAT 300
             I++QRK DL EGKASATQDILSHML+T DE+G++M E DIADKILGLLIGGHDT S+ 
Sbjct: 241 VGIIKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSA 300

Query: 301 CTFIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPL 360
           CTF+VKFLA+LP IY GVY EQMEI K+K  GELL WEDIQKMKYSWNVACEVLR+APPL
Sbjct: 301 CTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPL 360

Query: 361 QGAFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFV 420
           QGAFRE L+DF + GF IPKGWK+YWS+N+TH N E FPEP K +PSRF+G GP P++FV
Sbjct: 361 QGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFV 420

Query: 421 PFGGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           PFGGGPRMCPGKEYARLEILVF+H+LVKRFKW  ++ +EKI+V+P+P P  GLP+ LFPH
Sbjct: 421 PFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPH 479

[Close]

BLAST of Tan0002717 vs. ExPASy Swiss-Prot
Match: A0A3Q7HBJ5 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 6.6e-196
Identity = 325/478 (67.99%), Postives = 391/478 (81.80%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           ME   V L+ +FV L+SL   +L YK K   S   LPPG  G P+ GESLEFLSSG    
Sbjct: 1   MELLYVCLVCVFVFLVSL---LLLYKKK---SGEGLPPGKTGWPVFGESLEFLSSGWKGH 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+ KY S +FKT ++G  AA+FCG + NKFLFSNENKLV  WWP+SVNK+FPS
Sbjct: 61  PEKFIFDRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENKLVQAWWPNSVNKVFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           STQT+SKEEA K++K+LP F  PEALQRY+GIMD + QRHFA+ WEN+EQV+V PL   +
Sbjct: 121 STQTSSKEEAIKMRKMLPNFFKPEALQRYVGIMDHITQRHFATGWENKEQVVVFPLTKRY 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TF +ACRLF+SVED   + + +  F+ +  G+ S+PIDLPGT FN AIKASN +RKEL  
Sbjct: 181 TFWLACRLFLSVEDPKHVAKFADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELVR 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           I++QRK DL EGK S+TQDILSHML+TCDENG+++ + DIADKILGLLIGGHDT S+ C+
Sbjct: 241 IIKQRKIDLGEGKVSSTQDILSHMLLTCDENGKFLGDLDIADKILGLLIGGHDTASSACS 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVK+LA+LPHIY  VYTEQMEIAK+K PGELL WEDIQKMKYSWNVACEVLR+APPLQG
Sbjct: 301 FIVKYLAELPHIYQRVYTEQMEIAKSKGPGELLRWEDIQKMKYSWNVACEVLRLAPPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE L+DF+F GFYIPKGWKIYWS+N+THK +E+FP+P K +PSRFEG+GPAPYTFVPF
Sbjct: 361 AFREALSDFMFNGFYIPKGWKIYWSANSTHKREEFFPDPEKFDPSRFEGSGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYARLEILVF+H+LVKRFK+  ++ +EKIIV+P+P P  GLP+ L+PH
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHHLVKRFKFEKIIPHEKIIVNPMPIPANGLPLRLYPH 472

[Close]

BLAST of Tan0002717 vs. NCBI nr
Match: KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 815.5 bits (2105), Expect = 2.5e-232
Identity = 393/478 (82.22%), Postives = 426/478 (89.12%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           M  FL+SLLILF+SL+SLTLF+LFYKHKSLFSYPN PPG IG+PI+GESLEFLS+G    
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSDIFKTSI+G PAAIFCGPACNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           ST+ NSKE A +LKKLLP+FL PEALQRYIG+MDELAQRHFASSWENREQVLV PLA SF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVS+ED   +ERLS  F+ I  G+ SMPIDLPGT FN AIKASN VRKEL S
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRKSDLAEGKAS TQDILSHML+TCDE GQYMNES IAD ILGLLIGGHDTTSA CT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVK+LA+LP IY GVY EQMEIAKAKA GELL WEDIQKMKYSWNV CEVLR+A PLQG
Sbjct: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE +N FVF GFYIPKGWK+YWSSN+THKN  YFPEPYK +PSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LLD EKIIVDP+P P+KGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. NCBI nr
Match: XP_022960871.1 (beta-amyrin 28-oxidase [Cucurbita moschata])

HSP 1 Score: 815.1 bits (2104), Expect = 3.2e-232
Identity = 393/478 (82.22%), Postives = 426/478 (89.12%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           M  FL+SLLILF+SL+SLTLF+LFYKHKSLFSYPN PPG IG+PI+GESLEFLS+G    
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSDIFKTSI+G PAAIFCGPACNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           ST+ NSKE A +LKKLLP+FL PEALQRYIG+MDELAQRHFASSWENREQVLV PLA SF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVS+ED   +ERLS  F+ I  G+ SMPIDLPGT FN AIKASN VRKEL S
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRKSDLAEGKAS TQDILSHML+TCDE GQYMNES IAD ILGLLIGGHDTTSA CT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIV +LA+LP IY GVY EQMEIAKAKA GELL WEDIQKMKYSWNVACEVLR+A PLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE +N FVF GFYIPKGWK+YWSSN+THKN  YFPEPYK +PSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LLD EKIIVDP+P P+KGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. NCBI nr
Match: XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 812.4 bits (2097), Expect = 2.1e-231
Identity = 391/478 (81.80%), Postives = 425/478 (88.91%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           M  FL+SLLILF+S +SLTLF+LFYKHKSLFSYPN PPG IG+PI+GESLEFLS+G    
Sbjct: 1   MADFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSDIFKTSI+G PAAIFCGPACNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           ST+ NSKE A +LKKLLP+FL PEALQRYIG+MDELAQRHFASSWENREQVLV PLA SF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVS+ED   +ERLS  F+ I  G+ SMPIDLPGT FN AIKASN VRKEL S
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRKSDLAEGKAS TQDILSHML+TCDE GQYMNES IAD ILGLLIGGHDTTSA CT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVK+LA+LP IY  VY EQMEIAKAK  GELL WEDIQKMKYSWNVACEVLR+A PLQG
Sbjct: 301 FIVKYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE +NDFVF GFYIPKGWK+YWSSN+THKN  YFPEPYK +PSRFEGNGPAPYTFVPF
Sbjct: 361 AFREPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ L+D EKIIVDP+P P+KGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 809.7 bits (2090), Expect = 1.4e-230
Identity = 387/478 (80.96%), Postives = 425/478 (88.91%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           MEHFL+SLLI F+  LSLTLFILF+ HKSLFSYPN+PPG IG+PILGESLEFLS+G    
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR++KYKSD+FKTSI+G PAAIFCG ACNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           STQT+SKEEAKKLKK LPQFL PEALQRYIG+MDELAQRHFAS WENRE+VLV PLA SF
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVSVED   ++RLS  FE I  G+ S+PIDLPGT FN AIKAS  +RKEL +
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IV++R+ DLAEGKASATQDILSHML+TCDENG +MNESDIADKILGLLIGGHDT S  CT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVKFLA+LPHIYHGVYTEQMEIAK KAPGE L WEDI+KMKYSWNV CEVLR+A P QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE L DFVF GFYIPKGWK+YWS+N+THK+ EYFPEPYK EP+RFEGNGP PYTFVPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LL+ E IIVDP+P PQKGLPI LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPH 478

[Close]

BLAST of Tan0002717 vs. NCBI nr
Match: XP_022158061.1 (beta-amyrin 28-oxidase [Momordica charantia])

HSP 1 Score: 809.3 bits (2089), Expect = 1.8e-230
Identity = 387/478 (80.96%), Postives = 422/478 (88.28%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           MEHFLVSLLI FVS++ + LFILFYKHKS FSYPN+PPG IG PI+GESLEF S+G    
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKS IFKTSI+G PAA+FCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           STQT+S EEAKKLKKLLPQFL PEAL RYIGIMD LA RHFASSWEN++QVLV PLA SF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVSVED + +ERLS  F+ I  G+ SMPID PGT FN  IKASN +RKEL S
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IV+QRK DLAEGKAS TQDILSHML+TCDENG YMNE DIADKILGLLIGGHDT SA CT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVKFLA+LPHIY GVY EQM IAKAKAPGELLNWEDIQKMKYSWNVACEVLR+A PLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE +NDFVF GFYIPKGWK+YWSSN+THKN +YFP+PY  +PSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYARLEILVF+HNLVKRFKW+ +L+ EKI+VDP+P PQKGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. ExPASy TrEMBL
Match: A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.6e-232
Identity = 393/478 (82.22%), Postives = 426/478 (89.12%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           M  FL+SLLILF+SL+SLTLF+LFYKHKSLFSYPN PPG IG+PI+GESLEFLS+G    
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSDIFKTSI+G PAAIFCGPACNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           ST+ NSKE A +LKKLLP+FL PEALQRYIG+MDELAQRHFASSWENREQVLV PLA SF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVS+ED   +ERLS  F+ I  G+ SMPIDLPGT FN AIKASN VRKEL S
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRKSDLAEGKAS TQDILSHML+TCDE GQYMNES IAD ILGLLIGGHDTTSA CT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIV +LA+LP IY GVY EQMEIAKAKA GELL WEDIQKMKYSWNVACEVLR+A PLQG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE +N FVF GFYIPKGWK+YWSSN+THKN  YFPEPYK +PSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LLD EKIIVDP+P P+KGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. ExPASy TrEMBL
Match: A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 8.5e-231
Identity = 387/478 (80.96%), Postives = 422/478 (88.28%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           MEHFLVSLLI FVS++ + LFILFYKHKS FSYPN+PPG IG PI+GESLEF S+G    
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKS IFKTSI+G PAA+FCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           STQT+S EEAKKLKKLLPQFL PEAL RYIGIMD LA RHFASSWEN++QVLV PLA SF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLFVSVED + +ERLS  F+ I  G+ SMPID PGT FN  IKASN +RKEL S
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IV+QRK DLAEGKAS TQDILSHML+TCDENG YMNE DIADKILGLLIGGHDT SA CT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVKFLA+LPHIY GVY EQM IAKAKAPGELLNWEDIQKMKYSWNVACEVLR+A PLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE +NDFVF GFYIPKGWK+YWSSN+THKN +YFP+PY  +PSRFEGNGPAPYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYARLEILVF+HNLVKRFKW+ +L+ EKI+VDP+P PQKGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. ExPASy TrEMBL
Match: A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.9e-230
Identity = 388/478 (81.17%), Postives = 422/478 (88.28%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           MEHFL+SLLIL    LSLTLFILF+ HKSLFSYPN PPG IG+PILGESLEFLSSG    
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSD+FKTSI+G PAAIFCGP CNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           +TQT+SKEEAKKLKKLLPQFL PEALQRYIGIMDELA+RHF S W+NRE+VLV PLA SF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLF+SVED   +ERLS  FE I  G+ SMPIDLPGT FN AIKAS  +RKE+ +
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRK DLAEGKA ATQDILSHML+TCDENG YMNESDI DKILGLLIGGHDT S  CT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVKFLA+LPHIY  VYTEQMEIA+AKA GE L WEDI+KMKYSWNVACEVLR+A PLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE LNDFVF GF+IPKGWK+YWS+N+THKN EYFPEPYK EP RFEGNGP PYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LL+NE IIV+P+P PQKGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. ExPASy TrEMBL
Match: A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 4.0e-228
Identity = 388/480 (80.83%), Postives = 423/480 (88.12%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           ME FL+SLLILF+S +SLTLF+LFYKHKSLFSYPN PPG IG+PI+GESLEFLS+G    
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSDIFKTSI+G PAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           ST+ NSKE A +LKKLLP+FL PEALQRYIG+MDELAQRHFASSWENRE VLV PLA SF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFS+ACRLF+S+ED   +ERLS  F+ I  G+ SMPIDLPGT FN AIKASN VRKEL S
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRKSDLAEGKAS TQDILSHML+TCDE GQYMNES IAD ILGLLIGGHDT SA CT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEI--AKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPL 360
           FIVK+LA+LP IY  VY EQMEI  AKAKA GELL WEDIQKMKYSWNVACEVLR+A PL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 361 QGAFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFV 420
           QGAFRE +NDFVF GF IPKGWK+YWSSN+THKN  YFPEPYK +PSRFEGNGPAPYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 421 PFGGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           PFGGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LL+ EKIIVDP+P P+KGLP+ LFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

[Close]

BLAST of Tan0002717 vs. ExPASy TrEMBL
Match: A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 5.2e-228
Identity = 385/478 (80.54%), Postives = 421/478 (88.08%), Query Frame = 0


Query: 1   MEHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG---- 60
           ME FL+SLLIL    LSLTLFILF+ HKSLFSYPN PPG IG+PILGES+EFLSSG    
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 61  AEKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 120
            EKFIFDR+NKYKSD+FKTSI+G PAAIFCGP CNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 180
           +TQT++KEEAKKLKKLLPQFL PEALQRYIGIMDELA+RHF S W+NRE+VLV PLA SF
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 240
           TFSIACRLF+SVED   +ERLS  FE I  G+ SMPIDLPGT FN AIKAS  +RKE+ +
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 300
           IVRQRK DLAEGKA ATQDILSHML+TCDENG YMNESDI DKILGLLIGGHDT S  CT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 360
           FIVKFLA+LPHIY  VYTEQMEIA+AKA GE L WEDI+KMKYSWNVACEVLRIA PLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 361 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 420
           AFRE L+DFVF GF+IPKGWK+YWS+N+THKN EYFPEPYK +P RFEGNGP PYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFPH 475
           GGGPRMCPGKEYA+LEILVF+HNLVKRFKW+ LL+NE IIV+P+P PQKGLP+ LFPH
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPH 478

[Close]

BLAST of Tan0002717 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 620.9 bits (1600), Expect = 8.3e-178
Identity = 293/478 (61.30%), Postives = 365/478 (76.36%), Query Frame = 0


Query: 4   FLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG----AEK 63
           ++  ++ILF+S + L+L +L  KH S FSYPNLPPG  G+P++GES  FLS+G     EK
Sbjct: 2   YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61

Query: 64  FIFDRMNKYKSD---IFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPS 123
           FI DR+ ++ S    +FKT + G+P A+  G + NKFLF+NENKLV  WWPDSVNKIFPS
Sbjct: 62  FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121

Query: 124 STQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSF 183
           S QT+SKEEA+KL+ LL QF+ PEAL+RY+G+MDE+AQRHF + W N++QV+V PL   F
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 181

Query: 184 TFSIACRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRS 243
           TFSIACR F+S+ED +++ +L   F  +  G+FS+PIDLPGTRFN AIKAS L+RKE+ +
Sbjct: 182 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 241

Query: 244 IVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCT 303
           IVRQRK +L  GKA    DILSHML+   E      + D+ADKI+GLLIGGHDT S  CT
Sbjct: 242 IVRQRKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCT 301

Query: 304 FIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQG 363
           F+V +LA+ PH+Y  V  EQ EI K K   E L WEDI+KM+YSWNVACEV+RI PPL G
Sbjct: 302 FVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSG 361

Query: 364 AFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPF 423
            FRE ++ F FKGFYIPKGWK+YWS+ ATH N +YFPEP + EP+RFEG+GP PYT+VPF
Sbjct: 362 TFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPF 421

Query: 424 GGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNE-KIIVDPIPTPQKGLPICLFP 474
           GGGPRMCPGKEYARLEIL+F+HNLV RFKW  +  NE KI+VDP+P P KGLPI +FP
Sbjct: 422 GGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 475

[Close]

BLAST of Tan0002717 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 354.4 bits (908), Expect = 1.4e-97
Identity = 177/318 (55.66%), Postives = 229/318 (72.01%), Query Frame = 0


Query: 6   VSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSG----AEKFI 65
           ++++ LF+S +   L     KH S F YPNLPPG IG P++GE+L FLS+G     EKF+
Sbjct: 3   LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62

Query: 66  FDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNKIFPSSTQTN 125
            DR+  + S IFKT + G+P A+  G + NKFLF+NENKLV  WWPDSVNKIFPSSTQT+
Sbjct: 63  TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122

Query: 126 SKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSFTFSIA 185
           SKEEA K + LL   + PEAL+RY+G+MDE+AQ+HF + W N++Q++V PL   FTFSIA
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182

Query: 186 CRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRSIVRQR 245
           CRLF+S++D  ++ +L   F  +  GVFS+PIDLPGTRFN AIKAS L+ KE+ +I+RQR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242

Query: 246 KSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCTFIVKF 305
           K +L  GK S  QDILSHML+   E      + D+ADKI+ LLIGGHDTTS  CTF+V +
Sbjct: 243 KEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNY 302

Query: 306 LADLPHIYHGVYTEQMEI 320
           LA+ PHIY  V  E M+I
Sbjct: 303 LAEFPHIYQRV-LEGMQI 315

[Close]

BLAST of Tan0002717 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 270.0 bits (689), Expect = 3.6e-72
Identity = 159/475 (33.47%), Postives = 254/475 (53.47%), Query Frame = 0


Query: 2   EHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSGAEKFI 61
           E  L++L I+ V ++ L  + L +K + L     LPPG++G+P +GE+L   +     F 
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRLYTENPNSFF 78

Query: 62  FDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNK-IFPSSTQT 121
             R NKY  DIFKT I+G P  +   P   + +  ++  L  P +P S  + I P +   
Sbjct: 79  ATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFF 138

Query: 122 NSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSFTFSI 181
           +       LK+L+     P AL+  +  + EL      SSW +++ +        + F +
Sbjct: 139 HQGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFDV 198

Query: 182 A-CRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRSIVR 241
           A    F   E+ + ++ +   ++++ RG  SMP+DLPGT F+ ++KA   + +ELR ++ 
Sbjct: 199 AIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIE 258

Query: 242 QRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCTFIV 301
           +R+ +  E        +L  +L   D+    +++S IAD I+G++    DTT++  T+++
Sbjct: 259 KRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLL 318

Query: 302 KFLADLPHIYHGVYTEQMEI-AKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQGAF 361
           K+L D P++   V  EQ  I  K K     ++WED +KM  +  V  E LR A  L   F
Sbjct: 319 KYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTF 378

Query: 362 REVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPFGG 421
           RE + D  + G+ IPKGWK+       H + E+FP+P K +PSRFE   P PYT++PFG 
Sbjct: 379 REAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGN 438

Query: 422 GPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFP 474
           G   CPG E A+LE+L+ +H+L   F+W  + D E I   P P P+KGLPI + P
Sbjct: 439 GVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of Tan0002717 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 270.0 bits (689), Expect = 3.6e-72
Identity = 159/475 (33.47%), Postives = 254/475 (53.47%), Query Frame = 0


Query: 2   EHFLVSLLILFVSLLSLTLFILFYKHKSLFSYPNLPPGTIGIPILGESLEFLSSGAEKFI 61
           E  L++L I+ V ++ L  + L +K + L     LPPG++G+P +GE+L   +     F 
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRLYTENPNSFF 78

Query: 62  FDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWPDSVNK-IFPSSTQT 121
             R NKY  DIFKT I+G P  +   P   + +  ++  L  P +P S  + I P +   
Sbjct: 79  ATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFF 138

Query: 122 NSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVSPLATSFTFSI 181
           +       LK+L+     P AL+  +  + EL      SSW +++ +        + F +
Sbjct: 139 HQGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFDV 198

Query: 182 A-CRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASNLVRKELRSIVR 241
           A    F   E+ + ++ +   ++++ RG  SMP+DLPGT F+ ++KA   + +ELR ++ 
Sbjct: 199 AIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIE 258

Query: 242 QRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGHDTTSATCTFIV 301
           +R+ +  E        +L  +L   D+    +++S IAD I+G++    DTT++  T+++
Sbjct: 259 KRRENGRE-----EGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLL 318

Query: 302 KFLADLPHIYHGVYTEQMEI-AKAKAPGELLNWEDIQKMKYSWNVACEVLRIAPPLQGAF 361
           K+L D P++   V  EQ  I  K K     ++WED +KM  +  V  E LR A  L   F
Sbjct: 319 KYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTF 378

Query: 362 REVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGPAPYTFVPFGG 421
           RE + D  + G+ IPKGWK+       H + E+FP+P K +PSRFE   P PYT++PFG 
Sbjct: 379 REAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGN 438

Query: 422 GPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLPICLFP 474
           G   CPG E A+LE+L+ +H+L   F+W  + D E I   P P P+KGLPI + P
Sbjct: 439 GVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of Tan0002717 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 263.8 bits (673), Expect = 2.6e-70
Identity = 159/485 (32.78%), Postives = 259/485 (53.40%), Query Frame = 0


Query: 3   HFLVSLLILFV-SLLSLTLFILFYKH-----KSLFSYPNLPPGTIGIPILGESLEFLSSG 62
           +F++S + L + + +S TLF    KH     K +     L PG +G+P +GE+++F  + 
Sbjct: 6   NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65

Query: 63  A-----EKFIFDRMNKYKSDIFKTSIIGNPAAIFCGPACNKFLFSNENKLVTPWWP-DSV 122
                 E F+  R+ K+  +IFKT I+G+P  +  G   N+ + SNE  LV   WP  SV
Sbjct: 66  KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125

Query: 123 NKIFPSSTQTNSKEEAKKLKKLLPQFLNPEALQRYIGIMDELAQRHFASSWENREQVLVS 182
             +  +       E+ + L+ ++   L+   L+  I  + +  + H  + W  +E++ + 
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185

Query: 183 PLATSFTFSIA--CRLFVSVEDGSQMERLSRAFEKIGRGVFSMPIDLPGTRFNGAIKASN 242
             A   TF++   C   + VE G     +   FE++  GVF++P++ P ++F  A KA  
Sbjct: 186 RSAKVLTFTVVFECLYGIKVEIG-----MLEVFERVLEGVFALPVEFPCSKFARAKKARL 245

Query: 243 LVRKELRSIVRQRKSDLAEGKASATQDILSHMLVTCDENGQYMNESDIADKILGLLIGGH 302
            +   L   VR+++ ++ +  A      L   LV     G  + E ++ D ++ L+   H
Sbjct: 246 EIETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELIKG-VITEEEVVDNMVLLVFAAH 305

Query: 303 DTTSATCTFIVKFLADLPHIYHGVYTEQMEIAKAKAPGELLNWEDIQKMKYSWNVACEVL 362
           DTTS   +   K LA  P     +  E  +I   K  GE L  ED++KMKYSW V  E +
Sbjct: 306 DTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETM 365

Query: 363 RIAPPLQGAFREVLNDFVFKGFYIPKGWKIYWSSNATHKNKEYFPEPYKLEPSRFEGNGP 422
           R++PP+ G+FR+ + D  + G+ IPKGWKI W++  TH N E F +P   +P+RF+    
Sbjct: 366 RLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQ 425

Query: 423 APYTFVPFGGGPRMCPGKEYARLEILVFIHNLVKRFKWSNLLDNEKIIVDPIPTPQKGLP 474
           A YT++PFGGGPR+C G + A++ ILVF+H +V  F WS +  +E I +DP+P P  G+P
Sbjct: 426 A-YTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMP 482

The following BLAST results are available for this feature:

BLAST of Tan0002717 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6H9N6	8.0e-202	68.97	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1	[more]
A5BFI4	1.8e-201	68.83	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2	[more]
A0A0S2II38	6.4e-199	68.68	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
I7C6E8	3.9e-196	68.33	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]
A0A3Q7HBJ5	6.6e-196	67.99	Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 S...	[more]

BLAST of Tan0002717 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590124.1	2.5e-232	82.22	Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022960871.1	3.2e-232	82.22	beta-amyrin 28-oxidase [Cucurbita moschata]	[more]
XP_023516848.1	2.1e-231	81.80	beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo]	[more]
XP_038877759.1	1.4e-230	80.96	LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida]	[more]
XP_022158061.1	1.8e-230	80.96	beta-amyrin 28-oxidase [Momordica charantia]	[more]

BLAST of Tan0002717 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8T4	1.6e-232	82.22	beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1	[more]
A0A6J1DZX7	8.5e-231	80.96	beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1	[more]
A0A1S3B8H4	1.9e-230	81.17	beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1	[more]
A0A6J1JJQ7	4.0e-228	80.83	beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1	[more]
A0A0A0LXN3	5.2e-228	80.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1	[more]

BLAST of Tan0002717 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	8.3e-178	61.30	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT5G36140.1	1.4e-97	55.66	cytochrome P450, family 716, subfamily A, polypeptide 2	[more]
AT2G29090.1	3.6e-72	33.47	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	3.6e-72	33.47	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G42850.1	2.6e-70	32.78	cytochrome P450, family 718	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 286..303 score: 33.48 coord: 342..353 score: 35.23 coord: 423..434 score: 38.09 coord: 414..423 score: 58.31
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 37..454 e-value: 1.2E-56 score: 192.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 381..405 score: 21.64 coord: 423..446 score: 30.49 coord: 275..292 score: 28.51 coord: 413..423 score: 52.97
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 27..472 e-value: 4.1E-114 score: 383.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..473
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 11..454 e-value: 1.1E-6 score: 25.7
None	No IPR available	PANTHER	PTHR24286:SF297	BETA-AMYRIN 28-OXIDASE-LIKE	coord: 8..470
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 8..470
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 416..425

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0002717.1	Tan0002717.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen