Tan0002702 (gene) Snake gourd v1

Overview
NameTan0002702
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionglutelin type-A 2-like
LocationLG01: 7245881 .. 7247745 (+)
RNA-Seq ExpressionTan0002702
SyntenyTan0002702
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACCGATGAATCCCAAGCCCTTCACTGAGGGAGATGCTGGATCGTATCACAAATGGCTTCCTTCTGAATATCCCTTACTTGCTCAGACCAAGGTCGCCGCCGGCCGCCTTCTCCTCCGCCCTCGCGGCTTCGTCGTTCCCCACTACGCCGATTGCTCTAAAGTGGGCTATGTTCTTCAAGGTTAACCTCTCTATCTCTACTACTTTTAGTTCTCTTATGTCAATTTATAGTTTAAGATTCGAACAAGAAATTTTCTCATAATAATTGATAAGGACAACGGATCCTCTACCTCGTTCGTAATTTTGCTTTTTTTTTTTTGTTTAAGTCGAAATTAGACTTCATATAATGTCCCTTACTTAATTTATGATTTTTCTTCCTAATAGAATTTGCTTCAATTCATACAAAAATTTATGAAGGCGACAAATTTTATCATTCTCTCAACAATTTCGGTTGATTATTCCATATAAACTTTCTCTTGAAGATCTTCACATGAAAAAGACAAAAGAATTAAATAGATGTAAGGATCACAAATTTAAGACAACGAATTAATTAGAAAGAGAAATTTTTAAAGCTTTTAAATTTTTTTGCTCAATACTTTCAAAGTTGTTTTGGAATAATTTTTCAAATCTTTAGAAAATGGGTTTTTCATATGTAAAACAAAAAAAAAAGATTTTTGAGTATTTGAAAATTCATTTTAAACATGTTTGTGTAATCCATGCACGAGGGCGGGAGCTCTCTTCAAATTTTTTATCTTTATATAGAAAAAGTAAAGTCAAATAATAGTAAATATGTTATTAACCTCACCCAAGTCCCAAATTCAAGTTTAGTTTGTACAATGTTAATAATTTTTCCAATGTCATATTGAATCTATGTGTTTGCAGGTGAAGATGGGGTTGCAGGATTGGTGTTTCCAAACAAGTCCGATGAAGTGGTAGTGAAACTTAAGAAAGGAGATCTGATTCCGGTGCCGGAAGGAGTCACGTCGTGGTGGTTCAACGACGGAGACTCCGATTTCGAGATTATCTTTTTGGGTGAAACCAAAACCGCTCATGTCGCCGGTGACATCTCTTACTTCATTCTCTCCGGCCCTCTTGGCTTCCTGCAAGGCTTCTCGCCGGAGTACGTCGGAAAAACCTACTCTTTAAACGAAGAACAAACAACCACACTTCTCAAAAGCCAATCCAACGCCCTTATCTTCGCCCTTGCACAACCCCAATCCCTCCCCAAACCCCAAAAACACAGCAAACTAGTTTACAACATTGACGCCGCCGCGCCGGACACCACACCCAAGCCTAGCGGCGGCGGCGCCGTCACGACGGTGACGGAATCCAAATTTCCCTTCATTGGCCAATCTGGGTTGACGGCAATTCTTGAAAAGCTTGACGCCAACGCCGTTCGATCGCCGGTGTACGTTGCTGAGCCGTCCGATCAACTGATCTATGTGGCTAAAGGATTCGGGAAGATTCAGATTGTTGGATTTTCGAGTAAAGTTGATGCAGAGGTGAAAATGGGTCAGCTTATTTTAGTCCCCAAATACTTCGTCGCCGGAAAAATCGCCGGAGAAGAAGGCTTGGAGTGCTTCTCCATTATCACAGCTACACAGTAAAAACTAAAAAGCTTCAATTTTATTTTTTTTTTACTTTTTTATTTTTGGGGTTTTGTTTCTGAATCTTAAAATTTTTACATTTCTGATTTTGAATTGAACAGTCCTCTGGTGGAAGAATTGGCCGGAAAGACGTCGGTTTTCGAGGCATTGTCGCCGGAGATTCTTCAAGTTTCGTTCAACGTCACGGCGGAGTTCGAAAAGCTTCTTAGATCGAAGATCACAAAAACTTCACCAGTGATTCCACCTTCAGATTGA

mRNA sequence

ATGGAACCGATGAATCCCAAGCCCTTCACTGAGGGAGATGCTGGATCGTATCACAAATGGCTTCCTTCTGAATATCCCTTACTTGCTCAGACCAAGGTCGCCGCCGGCCGCCTTCTCCTCCGCCCTCGCGGCTTCGTCGTTCCCCACTACGCCGATTGCTCTAAAGTGGGCTATGTTCTTCAAGGTGAAGATGGGGTTGCAGGATTGGTGTTTCCAAACAAGTCCGATGAAGTGGTAGTGAAACTTAAGAAAGGAGATCTGATTCCGGTGCCGGAAGGAGTCACGTCGTGGTGGTTCAACGACGGAGACTCCGATTTCGAGATTATCTTTTTGGGTGAAACCAAAACCGCTCATGTCGCCGGTGACATCTCTTACTTCATTCTCTCCGGCCCTCTTGGCTTCCTGCAAGGCTTCTCGCCGGAGTACGTCGGAAAAACCTACTCTTTAAACGAAGAACAAACAACCACACTTCTCAAAAGCCAATCCAACGCCCTTATCTTCGCCCTTGCACAACCCCAATCCCTCCCCAAACCCCAAAAACACAGCAAACTAGTTTACAACATTGACGCCGCCGCGCCGGACACCACACCCAAGCCTAGCGGCGGCGGCGCCGTCACGACGGTGACGGAATCCAAATTTCCCTTCATTGGCCAATCTGGGTTGACGGCAATTCTTGAAAAGCTTGACGCCAACGCCGTTCGATCGCCGGTGTACGTTGCTGAGCCGTCCGATCAACTGATCTATGTGGCTAAAGGATTCGGGAAGATTCAGATTGTTGGATTTTCGAGTAAAGTTGATGCAGAGGTGAAAATGGGTCAGCTTATTTTAGTCCCCAAATACTTCGTCGCCGGAAAAATCGCCGGAGAAGAAGGCTTGGAGTGCTTCTCCATTATCACAGCTACACATCCTCTGGTGGAAGAATTGGCCGGAAAGACGTCGGTTTTCGAGGCATTGTCGCCGGAGATTCTTCAAGTTTCGTTCAACGTCACGGCGGAGTTCGAAAAGCTTCTTAGATCGAAGATCACAAAAACTTCACCAGTGATTCCACCTTCAGATTGA

Coding sequence (CDS)

ATGGAACCGATGAATCCCAAGCCCTTCACTGAGGGAGATGCTGGATCGTATCACAAATGGCTTCCTTCTGAATATCCCTTACTTGCTCAGACCAAGGTCGCCGCCGGCCGCCTTCTCCTCCGCCCTCGCGGCTTCGTCGTTCCCCACTACGCCGATTGCTCTAAAGTGGGCTATGTTCTTCAAGGTGAAGATGGGGTTGCAGGATTGGTGTTTCCAAACAAGTCCGATGAAGTGGTAGTGAAACTTAAGAAAGGAGATCTGATTCCGGTGCCGGAAGGAGTCACGTCGTGGTGGTTCAACGACGGAGACTCCGATTTCGAGATTATCTTTTTGGGTGAAACCAAAACCGCTCATGTCGCCGGTGACATCTCTTACTTCATTCTCTCCGGCCCTCTTGGCTTCCTGCAAGGCTTCTCGCCGGAGTACGTCGGAAAAACCTACTCTTTAAACGAAGAACAAACAACCACACTTCTCAAAAGCCAATCCAACGCCCTTATCTTCGCCCTTGCACAACCCCAATCCCTCCCCAAACCCCAAAAACACAGCAAACTAGTTTACAACATTGACGCCGCCGCGCCGGACACCACACCCAAGCCTAGCGGCGGCGGCGCCGTCACGACGGTGACGGAATCCAAATTTCCCTTCATTGGCCAATCTGGGTTGACGGCAATTCTTGAAAAGCTTGACGCCAACGCCGTTCGATCGCCGGTGTACGTTGCTGAGCCGTCCGATCAACTGATCTATGTGGCTAAAGGATTCGGGAAGATTCAGATTGTTGGATTTTCGAGTAAAGTTGATGCAGAGGTGAAAATGGGTCAGCTTATTTTAGTCCCCAAATACTTCGTCGCCGGAAAAATCGCCGGAGAAGAAGGCTTGGAGTGCTTCTCCATTATCACAGCTACACATCCTCTGGTGGAAGAATTGGCCGGAAAGACGTCGGTTTTCGAGGCATTGTCGCCGGAGATTCTTCAAGTTTCGTTCAACGTCACGGCGGAGTTCGAAAAGCTTCTTAGATCGAAGATCACAAAAACTTCACCAGTGATTCCACCTTCAGATTGA

Protein sequence

MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAGDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKHSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITATHPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD
Homology
BLAST of Tan0002702 vs. ExPASy Swiss-Prot
Match: P07730 (Glutelin type-A 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA2 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 8.8e-20
Identity = 86/395 (21.77%), Postives = 162/395 (41.01%), Query Frame = 0

Query: 31  TKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGEDGVAGLVFP------------------ 90
           T V+  R ++ PRG ++PHY + + + Y++QG  G+ G  FP                  
Sbjct: 80  TGVSVVRRVIEPRGLLLPHYTNGASLVYIIQGR-GITGPTFPGCPETYQQQFQQSGQAQL 139

Query: 91  ----------NKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAGD 150
                         + + + ++GD+I +P GV  W +NDG+     I++ +        D
Sbjct: 140 TESQSQSHKFKDEHQKIHRFRQGDVIALPAGVAHWCYNDGEVPVVAIYVTDINNGANQLD 199

Query: 151 ISY--FILSG---------------PLGFLQGFSPEYVGKTYSL-----------NEEQT 210
                F+L+G                     GFS E + + + +           N+++ 
Sbjct: 200 PRQRDFLLAGNKRNPQAYRREVEEWSQNIFSGFSTELLSEAFGISNQVARQLQCQNDQRG 259

Query: 211 TTLLKSQSNALIFALAQPQSLPKPQKHSKLVY---------------------------- 270
             +   +  +L+   A  Q   + Q  S+  Y                            
Sbjct: 260 EIVRVERGLSLLQPYASLQEQEQGQMQSREHYQEGGYQQSQYGSGCPNGLDETFCTMRVR 319

Query: 271 -NIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPSDQ 330
            NID      T  P   G VT +    FP +    ++A+   L  NA+ SP +    +  
Sbjct: 320 QNIDNPNRADTYNPR-AGRVTNLNSQNFPILNLVQMSAVKVNLYQNALLSPFWNIN-AHS 379

Query: 331 LIYVAKGFGKIQIVGFSSKV--DAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITATHP 339
           ++Y+ +G  ++Q+V  + K   + E++ GQL++VP+++V  K A  EG    +  T  + 
Sbjct: 380 IVYITQGRAQVQVVNNNGKTVFNGELRRGQLLIVPQHYVVVKKAQREGCAYIAFKTNPNS 439

BLAST of Tan0002702 vs. ExPASy Swiss-Prot
Match: Q09151 (Glutelin type-A 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA3 PE=2 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 2.0e-19
Identity = 94/408 (23.04%), Postives = 166/408 (40.69%), Query Frame = 0

Query: 31  TKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGEDGVAGLVFP------------------ 90
           T V   R ++ PRG ++PHY++ + + YV+QG  G+ G  FP                  
Sbjct: 79  TGVFVVRRVIEPRGLLLPHYSNGATLVYVIQGR-GITGPTFPGCPETYQQQFQQSEQDQQ 138

Query: 91  ----------NKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFL----------- 150
                         + + + ++GD++ +P GV  W +NDGD+    I++           
Sbjct: 139 LEGQSQSHKFRDEHQKIHRFQQGDVVALPAGVAHWCYNDGDAPIVAIYVTDIYNSANQLD 198

Query: 151 ---------GETK-------------TAHVAGDISYFILSGPLGFLQGFS---------- 210
                    G  K             + +V G  S  +LS  LG   G +          
Sbjct: 199 PRHRDFFLAGNNKIGQQLYRYEARDNSKNVFGGFSVELLSEALGISSGVARQLQCQNDQR 258

Query: 211 PEYVGKTYSLNEEQTTTLLKSQSNALIFA--LAQPQSLPKPQKHS------------KLV 270
            E V   + L+  Q    L+ Q    + +    Q Q   K  + S            ++ 
Sbjct: 259 GEIVRVEHGLSLLQPYASLQEQQQEQVQSRDYGQTQYQQKQLQGSCSNGLDETFCTMRVR 318

Query: 271 YNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPSDQ 330
            NID      T  P   G +T +   KFP +    ++A+   L  NA+ SP +    +  
Sbjct: 319 QNIDNPNLADTYNPR-AGRITYLNGQKFPILNLVQMSAVKVNLYQNALLSPFWNIN-AHS 378

Query: 331 LIYVAKGFGKIQIVGFSSKV--DAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITATHP 352
           ++Y+ +G  ++Q+V  + K   D E++ GQL+++P++ V  K A  EG    ++ T    
Sbjct: 379 VVYITQGRARVQVVNNNGKTVFDGELRRGQLLIIPQHHVVIKKAQREGCSYIALKTNPDS 438

BLAST of Tan0002702 vs. ExPASy Swiss-Prot
Match: Q8GZP6 (11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occidentale OX=171929 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.7e-18
Identity = 98/421 (23.28%), Postives = 179/421 (42.52%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           ++ + P    E +AG+   W P+ +       VA  R  ++P G ++P Y++  ++ YV+
Sbjct: 30  LDALEPDNRVEYEAGTVEAWDPN-HEQFRCAGVALVRHTIQPNGLLLPQYSNAPQLIYVV 89

Query: 61  QGEDGVAGLVFPNKSD---------------------EVVVKLKKGDLIPVPEGVTSWWF 120
           QGE G+ G+ +P   +                     + + + ++GD+I +P GV  W +
Sbjct: 90  QGE-GMTGISYPGCPETYQAPQQGRQQGQSGRFQDRHQKIRRFRRGDIIAIPAGVAHWCY 149

Query: 121 NDGDSDFEIIFLGETKTA-----------HVAGDISYFI------LSGPLGFLQGFSPEY 180
           N+G+S    + L +   +           H+AG+            S       GF  E 
Sbjct: 150 NEGNSPVVTVTLLDVSNSQNQLDRTPRKFHLAGNPKDVFQQQQQHQSRGRNLFSGFDTEL 209

Query: 181 VGKTYSLNEEQTTTLLKSQSN-------------ALIFALAQPQSLPKPQKHS------- 240
           + + + + +E+    LKS+ N              +  + +Q +   + ++ S       
Sbjct: 210 LAEAFQV-DERLIKQLKSEDNRGGIVKVKDDELRVIRPSRSQSERGSESEEESEDEKRRW 269

Query: 241 --------------KLVYNID--AAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILE 300
                         +L  NI+  A A   TP+    G +TT+     P +    L+    
Sbjct: 270 GQRDNGIEETICTMRLKENINDPARADIYTPEV---GRLTTLNSLNLPILKWLQLSVEKG 329

Query: 301 KLDANAVRSPVYVAEPSDQLIYVAKGFGKIQIV-GFSSKV-DAEVKMGQLILVPKYFVAG 346
            L  NA+  P +    S  +IY  KG G++Q+V  F ++V D EV+ GQ+++VP+ F   
Sbjct: 330 VLYKNALVLPHWNLN-SHSIIYGCKGKGQVQVVDNFGNRVFDGEVREGQMLVVPQNFAVV 389

BLAST of Tan0002702 vs. ExPASy Swiss-Prot
Match: Q9ZWA9 (12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.7e-18
Identity = 100/408 (24.51%), Postives = 165/408 (40.44%), Query Frame = 0

Query: 6   PKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGEDG 65
           P   T+ +AG    W     P L    V   R+ L+P    +P +     + YV+QGE G
Sbjct: 46  PAQATKFEAGQMEVW-DHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGE-G 105

Query: 66  VAGLV---FPNKSDEV----------------------VVKLKKGDLIPVPEGVTSWWFN 125
           V G +    P    EV                      +   ++GD+     GV+ WW+N
Sbjct: 106 VMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYN 165

Query: 126 DGDSDFEI-IFLGETKTAHVAGDI-SYFILSG--------PLGF------LQGFSPEYVG 185
            GDSD  I I L  T   +    +   F L+G        PL +        GF P  + 
Sbjct: 166 RGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIA 225

Query: 186 KTYSLNEEQTTTLLKSQSN---------ALIFALAQPQ---------SLPKPQKHSKLVY 245
           + + +N E    L   + N          L F +  P+          + +    +K+  
Sbjct: 226 EAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHE 285

Query: 246 NIDAAAPDTTPK-PSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPSDQ 305
           NID   P+ +    +  G ++T+     P +    L A+   L +  +  P + A  +  
Sbjct: 286 NID--DPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTAN-AHT 345

Query: 306 LIYVAKGFGKIQIV--GFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITATHP 352
           ++YV  G  KIQ+V     S  + +V  GQ+I++P+ F   K AGE G E  S  T  + 
Sbjct: 346 VLYVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNA 405

BLAST of Tan0002702 vs. ExPASy Swiss-Prot
Match: P07728 (Glutelin type-A 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA1 PE=1 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 3.7e-18
Identity = 84/395 (21.27%), Postives = 159/395 (40.25%), Query Frame = 0

Query: 31  TKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGEDGVAGLVFP------------------ 90
           T V+  R ++ PRG ++PHY + + + Y++QG  G+ G  FP                  
Sbjct: 80  TGVSVVRRVIEPRGLLLPHYTNGASLVYIIQGR-GITGPTFPGCPESYQQQFQQSGQAQL 139

Query: 91  ----------NKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAGD 150
                         + + + ++GD+I +P GV  W +NDG+     I++ +        D
Sbjct: 140 TESQSQSQKFKDEHQKIHRFRQGDVIALPAGVAHWCYNDGEVPVVAIYVTDLNNGANQLD 199

Query: 151 ISY--FILSG---------------PLGFLQGFSPEYVGKTYSL-----------NEEQT 210
                F+L+G                     GFS E + +   +           N+++ 
Sbjct: 200 PRQRDFLLAGNKRNPQAYRREVEERSQNIFSGFSTELLSEALGVSSQVARQLQCQNDQRG 259

Query: 211 TTLLKSQSNALIFALAQPQSLPKPQKHSKLVY---------------------------- 270
             +      +L+   A  Q   + Q  S+  Y                            
Sbjct: 260 EIVRVEHGLSLLQPYASLQEQEQGQVQSRERYQEGQYQQSQYGSGCSNGLDETFCTLRVR 319

Query: 271 -NIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPSDQ 330
            NID      T  P   G VT +    FP +    ++A+   L  NA+ SP +    +  
Sbjct: 320 QNIDNPNRADTYNPR-AGRVTNLNTQNFPILSLVQMSAVKVNLYQNALLSPFWNIN-AHS 379

Query: 331 LIYVAKGFGKIQIVGFSSKV--DAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITATHP 339
           ++Y+ +G  ++Q+V  + K   + E++ GQL+++P+++   K A  EG    +  T  + 
Sbjct: 380 VVYITQGRARVQVVNNNGKTVFNGELRRGQLLIIPQHYAVVKKAQREGCAYIAFKTNPNS 439

BLAST of Tan0002702 vs. NCBI nr
Match: XP_022922755.1 (legumin J-like [Cucurbita moschata])

HSP 1 Score: 594.3 bits (1531), Expect = 6.6e-166
Identity = 300/351 (85.47%), Postives = 316/351 (90.03%), Query Frame = 0

Query: 2   EPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 61
           +PMNPKPFTE +AGSYHKWLPSEYPLLAQ KVAAGRLLLRPRGFVVPHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAG 121
           GE+GVAGLVFP+KSDEVVV LKKGDLIPVP GV+SWWFNDGDSD EIIFLGE+K AHV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKH 181
           DISYF+LSGPL  L GFSPEYVGKTYSLN E+TT  LKSQSNALIF++ Q QSLPKP K+
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKY 182

Query: 182 SKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 241
           SK VYNIDAAAPD   K  G GAVTTVTESKFPFIGQSGLTAILEKL+ANAVRSPVYVAE
Sbjct: 183 SKFVYNIDAAAPDGRVK-GGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVAE 242

Query: 242 PSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITAT 301
           P DQLIYVAKG GKIQIVG SSK+DAEVKMGQLILVPK+F  GKIAGE+GLEC SIITAT
Sbjct: 243 PYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITAT 302

Query: 302 HPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           HP+VEELAGKTSV EALSPEI QVSFNVTAEFEKLLRSKIT  SPVI  SD
Sbjct: 303 HPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of Tan0002702 vs. NCBI nr
Match: KAG6576976.1 (12S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.0 bits (1525), Expect = 3.3e-165
Identity = 299/351 (85.19%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 2   EPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 61
           +PMNPKPFTE +AGSYHKWLPSEYPLLA+ KVAAGRLLLRPRGFVVPHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAG 121
           GE+GVAGLVFP+KSDEVVV LKKGDLIPVP GV+SWWFN+GDSD EIIFLGE+K AHV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNEGDSDLEIIFLGESKNAHVPG 122

Query: 122 DISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKH 181
           DISYF+LSGPL  L GFSPEYVGKTYSLN E+TT  LKSQSNALIF++ Q QSLPKP K 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKF 182

Query: 182 SKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 241
           SK VYNIDAAAPD   K  G GAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE
Sbjct: 183 SKFVYNIDAAAPDGRVK-GGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 242

Query: 242 PSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITAT 301
           P DQLIYVAKG GKIQIVG SSK+DAEVKMGQLILVPK+F  GKIAGE+GLEC SIITAT
Sbjct: 243 PYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITAT 302

Query: 302 HPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           HP+VEELAGKTSV EALSPEI QVSFNVTAEFEKLLRSKIT  SPVI  SD
Sbjct: 303 HPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of Tan0002702 vs. NCBI nr
Match: XP_022985328.1 (12S seed storage protein CRD-like [Cucurbita maxima])

HSP 1 Score: 591.3 bits (1523), Expect = 5.6e-165
Identity = 298/351 (84.90%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 2   EPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 61
           +PMNPKPFTE +AGSYHKWLPSEYPLLA  KVAAGRLLLRPRGFVVPHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAG 121
           GE+GVAGLVFP+KSDEVVV LKKGDLIPVP GV+SWWFNDGDSD EIIFLGE+K AHV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKH 181
           DISYF+LSG L  L GFSPEYVG+TYSLN E+TT  LKSQSNALIF++ Q QSLPKP K+
Sbjct: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182

Query: 182 SKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 241
           SK VYNIDAAAPD   K  G GAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE
Sbjct: 183 SKFVYNIDAAAPDGRVK-GGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 242

Query: 242 PSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITAT 301
           P DQLIYVAKG GKIQIVGFSSK+DAEVKMGQLILVPK+F  GKIAGE+GLEC SIITAT
Sbjct: 243 PYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITAT 302

Query: 302 HPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           HP+VEELAGKTSV EALSPE+ QVSFNVTAEFEKLLRSKIT  SPVI  SD
Sbjct: 303 HPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 352

BLAST of Tan0002702 vs. NCBI nr
Match: XP_023552908.1 (12S seed storage globulin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-163
Identity = 296/351 (84.33%), Postives = 312/351 (88.89%), Query Frame = 0

Query: 2   EPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 61
           +PMNPKPFTE +AGSYHKWLPSEYPLLA+ KVAAGRLLLRPRGFVVPHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAG 121
           GE+GV GLVFP+KSDEVVV LKKGDLIPVP GV+SWWFNDGDSD EIIFLGE+K AHV G
Sbjct: 63  GENGVVGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKH 181
           DISYF+LSGPL  L GFSPEYVGKTYSLN E+TT  LKSQSNALI ++ Q QSLPKP K 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALICSIQQTQSLPKPPKF 182

Query: 182 SKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 241
           SK VYNIDAAAPD   K S  GAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE
Sbjct: 183 SKFVYNIDAAAPDGRVKGS-AGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 242

Query: 242 PSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITAT 301
           P DQLIYVAKG GKIQIVG SSK+DAEVKMGQLILVPK+F  GK AGE+GLEC SIITAT
Sbjct: 243 PYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKFAGEDGLECISIITAT 302

Query: 302 HPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           HP+VEELAGKTSV EALSPE+ QVSFNVTAEFEKLLRSKIT  SPVI  SD
Sbjct: 303 HPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of Tan0002702 vs. NCBI nr
Match: XP_008456076.1 (PREDICTED: glutelin type-A 2-like [Cucumis melo] >KAA0039043.1 glutelin type-A 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 548.5 bits (1412), Expect = 4.2e-152
Identity = 268/341 (78.59%), Postives = 293/341 (85.92%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           ME MNPKPF EG+ GSY KWLPS+YPLLAQT VA GRLLLRPRGF VPHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVA 120
           QGEDGV G VFPNK +EVV+KLKKGDLIPVP G+TSWWFNDGDSD EIIFLGETK AHV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQK 180
           GDI+YFILSGP G LQGF+PEYV K+YSL++E+T   LKSQSN LIF +   QSLPKP K
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHK 180

Query: 181 HSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           HSKLVYNIDAA PD   K  G  AVT VTES FPFIGQ+GLTA+LEKLDANA+RSPVY+A
Sbjct: 181 HSKLVYNIDAAVPDNRAK-VGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIA 240

Query: 241 EPSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITA 300
           EPSDQLIYV KG GKIQ+VGFSSK DA+VK+GQLILVP+YF  GK+AGEEGLEC S+I A
Sbjct: 241 EPSDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVA 300

Query: 301 THPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKI 342
           THP+VEELAGKTSV EALS E+ QVSFNVTAEFEKL RSK+
Sbjct: 301 THPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

BLAST of Tan0002702 vs. ExPASy TrEMBL
Match: A0A6J1E9P2 (legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.2e-166
Identity = 300/351 (85.47%), Postives = 316/351 (90.03%), Query Frame = 0

Query: 2   EPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 61
           +PMNPKPFTE +AGSYHKWLPSEYPLLAQ KVAAGRLLLRPRGFVVPHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAG 121
           GE+GVAGLVFP+KSDEVVV LKKGDLIPVP GV+SWWFNDGDSD EIIFLGE+K AHV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKH 181
           DISYF+LSGPL  L GFSPEYVGKTYSLN E+TT  LKSQSNALIF++ Q QSLPKP K+
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKY 182

Query: 182 SKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 241
           SK VYNIDAAAPD   K  G GAVTTVTESKFPFIGQSGLTAILEKL+ANAVRSPVYVAE
Sbjct: 183 SKFVYNIDAAAPDGRVK-GGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVAE 242

Query: 242 PSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITAT 301
           P DQLIYVAKG GKIQIVG SSK+DAEVKMGQLILVPK+F  GKIAGE+GLEC SIITAT
Sbjct: 243 PYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITAT 302

Query: 302 HPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           HP+VEELAGKTSV EALSPEI QVSFNVTAEFEKLLRSKIT  SPVI  SD
Sbjct: 303 HPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of Tan0002702 vs. ExPASy TrEMBL
Match: A0A6J1JDB2 (12S seed storage protein CRD-like OS=Cucurbita maxima OX=3661 GN=LOC111483370 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.7e-165
Identity = 298/351 (84.90%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 2   EPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 61
           +PMNPKPFTE +AGSYHKWLPSEYPLLA  KVAAGRLLLRPRGFVVPHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVAG 121
           GE+GVAGLVFP+KSDEVVV LKKGDLIPVP GV+SWWFNDGDSD EIIFLGE+K AHV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQKH 181
           DISYF+LSG L  L GFSPEYVG+TYSLN E+TT  LKSQSNALIF++ Q QSLPKP K+
Sbjct: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182

Query: 182 SKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 241
           SK VYNIDAAAPD   K  G GAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE
Sbjct: 183 SKFVYNIDAAAPDGRVK-GGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 242

Query: 242 PSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITAT 301
           P DQLIYVAKG GKIQIVGFSSK+DAEVKMGQLILVPK+F  GKIAGE+GLEC SIITAT
Sbjct: 243 PYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITAT 302

Query: 302 HPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           HP+VEELAGKTSV EALSPE+ QVSFNVTAEFEKLLRSKIT  SPVI  SD
Sbjct: 303 HPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 352

BLAST of Tan0002702 vs. ExPASy TrEMBL
Match: A0A5A7T7U8 (Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001330 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 268/341 (78.59%), Postives = 293/341 (85.92%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           ME MNPKPF EG+ GSY KWLPS+YPLLAQT VA GRLLLRPRGF VPHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVA 120
           QGEDGV G VFPNK +EVV+KLKKGDLIPVP G+TSWWFNDGDSD EIIFLGETK AHV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQK 180
           GDI+YFILSGP G LQGF+PEYV K+YSL++E+T   LKSQSN LIF +   QSLPKP K
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHK 180

Query: 181 HSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           HSKLVYNIDAA PD   K  G  AVT VTES FPFIGQ+GLTA+LEKLDANA+RSPVY+A
Sbjct: 181 HSKLVYNIDAAVPDNRAK-VGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIA 240

Query: 241 EPSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITA 300
           EPSDQLIYV KG GKIQ+VGFSSK DA+VK+GQLILVP+YF  GK+AGEEGLEC S+I A
Sbjct: 241 EPSDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVA 300

Query: 301 THPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKI 342
           THP+VEELAGKTSV EALS E+ QVSFNVTAEFEKL RSK+
Sbjct: 301 THPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

BLAST of Tan0002702 vs. ExPASy TrEMBL
Match: A0A1S3C2D5 (glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 268/341 (78.59%), Postives = 293/341 (85.92%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           ME MNPKPF EG+ GSY KWLPS+YPLLAQT VA GRLLLRPRGF VPHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVA 120
           QGEDGV G VFPNK +EVV+KLKKGDLIPVP G+TSWWFNDGDSD EIIFLGETK AHV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQK 180
           GDI+YFILSGP G LQGF+PEYV K+YSL++E+T   LKSQSN LIF +   QSLPKP K
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHK 180

Query: 181 HSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           HSKLVYNIDAA PD   K  G  AVT VTES FPFIGQ+GLTA+LEKLDANA+RSPVY+A
Sbjct: 181 HSKLVYNIDAAVPDNRAK-VGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIA 240

Query: 241 EPSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITA 300
           EPSDQLIYV KG GKIQ+VGFSSK DA+VK+GQLILVP+YF  GK+AGEEGLEC S+I A
Sbjct: 241 EPSDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVA 300

Query: 301 THPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKI 342
           THP+VEELAGKTSV EALS E+ QVSFNVTAEFEKL RSK+
Sbjct: 301 THPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

BLAST of Tan0002702 vs. ExPASy TrEMBL
Match: A0A0A0L6K0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G218160 PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.3e-151
Identity = 268/341 (78.59%), Postives = 291/341 (85.34%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           ME MNPKPF EG+ GSYHKWLPS+YPLLAQT VA GRLLLRPRGF VPHY+DCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYHKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYSDCSKFGYVL 60

Query: 61  QGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVA 120
           QGEDGV G VFP K +EVV+KLKKGDLIPVP GVTSWWFNDGDSD EIIFLGETK AHV 
Sbjct: 61  QGEDGVTGFVFPKKCNEVVIKLKKGDLIPVPAGVTSWWFNDGDSDLEIIFLGETKRAHVP 120

Query: 121 GDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQK 180
           GDI+YFILSGP G LQGF+PEYV K+ SLN+E+T T LKSQ N LIF +   QSLPKP K
Sbjct: 121 GDITYFILSGPRGLLQGFTPEYVQKSCSLNQEETNTFLKSQPNVLIFTVQPSQSLPKPHK 180

Query: 181 HSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           +SKLVYNIDAAAPD   K  G  AVT VTES FPFIGQ+GLT +LEKLDANA+RSPVY+A
Sbjct: 181 YSKLVYNIDAAAPDNRAK-VGDAAVTMVTESTFPFIGQTGLTPVLEKLDANAIRSPVYIA 240

Query: 241 EPSDQLIYVAKGFGKIQIVGFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITA 300
           EPSDQLIYV KG GKIQ+VGFSSK DA+VK GQLILVP+YF  GKIAGEEGLEC S+I A
Sbjct: 241 EPSDQLIYVTKGSGKIQVVGFSSKFDADVKTGQLILVPRYFAVGKIAGEEGLECISMIVA 300

Query: 301 THPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKI 342
           THP+VEELAGKTSV EALS E+ QVSFNVTAEFEKL RSK+
Sbjct: 301 THPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

BLAST of Tan0002702 vs. TAIR 10
Match: AT1G07750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.9e-71
Identity = 138/354 (38.98%), Postives = 204/354 (57.63%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           + P  PK    GD GSY  W P E P+L Q  + A +L L   GF VP Y+D SKV YVL
Sbjct: 5   LTPKLPKKVYGGDGGSYSAWCPEELPMLKQGNIGAAKLALEKNGFAVPRYSDSSKVAYVL 64

Query: 61  QGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVA 120
           QG  G AG+V P K +E V+ +K+GD I +P GV +WWFN+ D +  I+FLGET   H A
Sbjct: 65  QG-SGTAGIVLPEK-EEKVIAIKQGDSIALPFGVVTWWFNNEDPELVILFLGETHKGHKA 124

Query: 121 GDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQK 180
           G  + F L+G  G   GFS E+VG+ + L+E     L+ SQ+   I  L     +P+P++
Sbjct: 125 GQFTEFYLTGTNGIFTGFSTEFVGRAWDLDENTVKKLVGSQTGNGIVKLDAGFKMPQPKE 184

Query: 181 HSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
            ++  + ++            GG V  +     P +G+ G  A L ++DA+++ SP +  
Sbjct: 185 ENRAGFVLNCLEAPLDVDIKDGGRVVVLNTKNLPLVGEVGFGADLVRIDAHSMCSPGFSC 244

Query: 241 EPSDQLIYVAKGFGKIQIVGFSSK--VDAEVKMGQLILVPKYFVAGKIAGEEGLECFSII 300
           + + Q+ Y+  G G++Q+VG   K  ++  +K G L +VP++FV  KIA  +G+  FSI+
Sbjct: 245 DSALQVTYIVGGSGRVQVVGGDGKRVLETHIKAGSLFIVPRFFVVSKIADADGMSWFSIV 304

Query: 301 TATHPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           T   P+   LAG TSV+++LSPE+LQ +F V  E EK  RS  T ++   PPS+
Sbjct: 305 TTPDPIFTHLAGNTSVWKSLSPEVLQAAFKVAPEVEKSFRSTRTSSAIFFPPSN 356

BLAST of Tan0002702 vs. TAIR 10
Match: AT2G28680.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-69
Identity = 137/354 (38.70%), Postives = 200/354 (56.50%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           + P  PK    GD GSY  W P E P+L    + A +L L   G  +P Y+D  KV YVL
Sbjct: 5   LSPRLPKKVYGGDGGSYFAWCPEELPMLRDGNIGASKLALEKYGLALPRYSDSPKVAYVL 64

Query: 61  QGEDGVAGLVFPNKSDEVVVKLKKGDLIPVPEGVTSWWFNDGDSDFEIIFLGETKTAHVA 120
           QG  G AG+V P K +E V+ +KKGD I +P GV +WWFN+ D++  ++FLGET   H A
Sbjct: 65  QGA-GTAGIVLPEK-EEKVIAIKKGDSIALPFGVVTWWFNNEDTELVVLFLGETHKGHKA 124

Query: 121 GDISYFILSGPLGFLQGFSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQPQSLPKPQK 180
           G  + F L+G  G   GFS E+VG+ + L+E     L+ SQ+   I  +     +P+P+K
Sbjct: 125 GQFTDFYLTGSNGIFTGFSTEFVGRAWDLDETTVKKLVGSQTGNGIVKVDASLKMPEPKK 184

Query: 181 HSKLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
             +  + ++            GG V  +     P +G+ G  A L ++D +++ SP +  
Sbjct: 185 GDRKGFVLNCLEAPLDVDIKDGGRVVVLNTKNLPLVGEVGFGADLVRIDGHSMCSPGFSC 244

Query: 241 EPSDQLIYVAKGFGKIQIVGFSSK--VDAEVKMGQLILVPKYFVAGKIAGEEGLECFSII 300
           + + Q+ Y+  G G++QIVG   K  ++  VK G L +VP++FV  KIA  +GL  FSI+
Sbjct: 245 DSALQVTYIVGGSGRVQIVGADGKRVLETHVKAGVLFIVPRFFVVSKIADSDGLSWFSIV 304

Query: 301 TATHPLVEELAGKTSVFEALSPEILQVSFNVTAEFEKLLRSKITKTSPVIPPSD 353
           T   P+   LAG+TSV++ALSPE+LQ +F V  E EK  RSK T  +    PS+
Sbjct: 305 TTPDPIFTHLAGRTSVWKALSPEVLQAAFKVDPEVEKAFRSKRTSDAIFFSPSN 356

BLAST of Tan0002702 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 2.6e-19
Identity = 100/408 (24.51%), Postives = 165/408 (40.44%), Query Frame = 0

Query: 6   PKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGEDG 65
           P   T+ +AG    W     P L    V   R+ L+P    +P +     + YV+QGE G
Sbjct: 46  PAQATKFEAGQMEVW-DHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGE-G 105

Query: 66  VAGLV---FPNKSDEV----------------------VVKLKKGDLIPVPEGVTSWWFN 125
           V G +    P    EV                      +   ++GD+     GV+ WW+N
Sbjct: 106 VMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYN 165

Query: 126 DGDSDFEI-IFLGETKTAHVAGDI-SYFILSG--------PLGF------LQGFSPEYVG 185
            GDSD  I I L  T   +    +   F L+G        PL +        GF P  + 
Sbjct: 166 RGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIA 225

Query: 186 KTYSLNEEQTTTLLKSQSN---------ALIFALAQPQ---------SLPKPQKHSKLVY 245
           + + +N E    L   + N          L F +  P+          + +    +K+  
Sbjct: 226 EAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHE 285

Query: 246 NIDAAAPDTTPK-PSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPSDQ 305
           NID   P+ +    +  G ++T+     P +    L A+   L +  +  P + A  +  
Sbjct: 286 NID--DPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTAN-AHT 345

Query: 306 LIYVAKGFGKIQIV--GFSSKVDAEVKMGQLILVPKYFVAGKIAGEEGLECFSIITATHP 352
           ++YV  G  KIQ+V     S  + +V  GQ+I++P+ F   K AGE G E  S  T  + 
Sbjct: 346 VLYVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNA 405

BLAST of Tan0002702 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-17
Identity = 90/411 (21.90%), Postives = 164/411 (39.90%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           +  + P    + + G    W     P L  +  A  R ++ P+G  +P + +  K+ +V+
Sbjct: 35  LNALEPSQIIKSEGGRIEVW-DHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVV 94

Query: 61  QGEDGVAGLVFPNKSD------------------------EVVVKLKKGDLIPVPEGVTS 120
            G  G+ G V P  ++                        + V  L+ GD I  P GV  
Sbjct: 95  HGR-GLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQ 154

Query: 121 WWFNDGDSDFEIIFLGE--TKTAHVAGDISYFILSG--PLG--------------FLQGF 180
           W++N+G+    ++   +  +    +  ++  F+++G  P G                 GF
Sbjct: 155 WFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGF 214

Query: 181 SPEYVGKTYSLNEEQTTTLLKSQSN-ALIFALAQPQSLPKP---------QKHS------ 240
           +PE + + + +N E    L   Q N   I  +  P  + +P         Q H       
Sbjct: 215 APEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLE 274

Query: 241 ------KLVYNIDAAAPDTTPKPSGGGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSP 300
                 +   N+D  +     KPS  G ++T+     P +    L+A+   +  NA+  P
Sbjct: 275 ETLCTMRCTENLDDPSDADVYKPS-LGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLP 334

Query: 301 VYVAEPSDQLIYVAKGFGKIQIVGFSSK--VDAEVKMGQLILVPKYFVAGKIAGEEGLEC 346
            +    ++  +YV  G   IQ+V  + +   D E+  GQL++VP+ F   K A  E  E 
Sbjct: 335 QWNVN-ANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEW 394

BLAST of Tan0002702 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 3.3e-14
Identity = 86/417 (20.62%), Postives = 156/417 (37.41%), Query Frame = 0

Query: 1   MEPMNPKPFTEGDAGSYHKWLPSEYPLLAQTKVAAGRLLLRPRGFVVPHYADCSKVGYVL 60
           +  + P    + +AG    W     P L  + V+  R ++  +G  +P + + +K+ +V 
Sbjct: 41  LNALEPSHVLKSEAGRIEVW-DHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAKLSFVA 100

Query: 61  QGEDGVAGLVFPNKSD-------------------------EVVVKLKKGDLIPVPEGVT 120
           +G  G+ G V P  ++                         + V  ++ GD I    GV 
Sbjct: 101 KGR-GLMGKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVA 160

Query: 121 SWWFNDGDSDFEIIFLGETKTAHVAGDIS----YFILSGPLG--------------FLQG 180
            W++NDG     I+ + +  +     D +    Y   + P G                 G
Sbjct: 161 QWFYNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQKNIFNG 220

Query: 181 FSPEYVGKTYSLNEEQTTTLLKSQSNALIFALAQ--------PQSLPKPQ-------KHS 240
           F PE + +   ++ +    L     N       Q        P    +PQ       +H 
Sbjct: 221 FGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRHG 280

Query: 241 KLVYNID-----AAAPDTTPKPSGG-------GAVTTVTESKFPFIGQSGLTAILEKLDA 300
           +    ++     A   D    PS         G ++T+     P +    L+A+   +  
Sbjct: 281 RHGNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQ 340

Query: 301 NAVRSPVYVAEPSDQLIYVAKGFGKIQIVGFSSK--VDAEVKMGQLILVPKYFVAGKIAG 346
           NA+  P + A  ++ ++YV  G  +IQIV  +     D +V  GQLI VP+ F   K A 
Sbjct: 341 NAMVLPQWNAN-ANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVKRAT 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P077308.8e-2021.77Glutelin type-A 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA2 PE=1 SV=1[more]
Q091512.0e-1923.04Glutelin type-A 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA3 PE=2 SV=2[more]
Q8GZP63.7e-1823.2811S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occident... [more]
Q9ZWA93.7e-1824.5112S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1[more]
P077283.7e-1821.27Glutelin type-A 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022922755.16.6e-16685.47legumin J-like [Cucurbita moschata][more]
KAG6576976.13.3e-16585.1912S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022985328.15.6e-16584.9012S seed storage protein CRD-like [Cucurbita maxima][more]
XP_023552908.14.0e-16384.3312S seed storage globulin 1-like [Cucurbita pepo subsp. pepo][more]
XP_008456076.14.2e-15278.59PREDICTED: glutelin type-A 2-like [Cucumis melo] >KAA0039043.1 glutelin type-A 2... [more]
Match NameE-valueIdentityDescription
A0A6J1E9P23.2e-16685.47legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1[more]
A0A6J1JDB22.7e-16584.9012S seed storage protein CRD-like OS=Cucurbita maxima OX=3661 GN=LOC111483370 PE... [more]
A0A5A7T7U82.0e-15278.59Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3C2D52.0e-15278.59glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1[more]
A0A0A0L6K01.3e-15178.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G218160 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G07750.13.9e-7138.98RmlC-like cupins superfamily protein [more]
AT2G28680.13.6e-6938.70RmlC-like cupins superfamily protein [more]
AT1G03890.12.6e-1924.51RmlC-like cupins superfamily protein [more]
AT1G03880.11.1e-1721.90cruciferin 2 [more]
AT5G44120.33.3e-1420.62RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 186..335
e-value: 7.7E-12
score: 55.3
coord: 2..155
e-value: 4.3E-26
score: 102.7
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 200..333
e-value: 2.5E-15
score: 56.4
coord: 5..154
e-value: 2.8E-20
score: 72.4
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 5..174
e-value: 6.2E-30
score: 105.8
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 194..352
e-value: 9.7E-36
score: 124.5
NoneNo IPR availablePANTHERPTHR31189:SF4511S GLOBULIN SEED STORAGE PROTEIN 2-LIKEcoord: 1..343
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 1..343
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 3..163
e-value: 1.97076E-57
score: 183.555
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 201..349
e-value: 8.89742E-60
score: 187.681
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 4..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002702.1Tan0002702.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0045735 nutrient reservoir activity