Tan0002450 (gene) Snake gourd v1

Overview
NameTan0002450
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein KINESIN LIGHT CHAIN-RELATED 1
LocationLG06: 10400337 .. 10404212 (-)
RNA-Seq ExpressionTan0002450
SyntenyTan0002450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTGCAAAGGTTTAGGCTGAAACAACCAAAAACCTCGCAAAAGCGCAGACTATTCACTATCACTTTCAATCCCACAATGAGAAGAAACGCTTCGATTTCACTTCTCTCTCACTCCGATCGTCTCAATCTCCGCTCACTTTCATCCCTCATTTCCCCAAATTCCATTTCCAATCCTTCCCCTTCGTATTCCTCCCCTGTTTCAACCCCCCATCACAAGCCATGCACCAAAAACCATGGTTTGTTCTTCAAATTCACCCGATTTCAAGCAAGCCCATCTCGAAATTTGGATACCCTTGTGGAGAAACGCCCTCAAATTTCCTCCAGACAGCGCAAAGTCAGAGAGAAATCCGAGCTTGAGGAGGCTTTTGAGTCTGCAAACACTGTTGATGAAATGCTCAAGGCGTTTAAGGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGACCCTGAAAAGGCTCTGTTGTACGCTGGTAGAGCTTTGAATGCTTTTGATAAAGATGATAATGGGAAACCTTCTTTACTTGTTGCTATGACTTTACAATTGATGGGTTCTGTTAATTATAGCTTGAAAAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCTAATAGGATATTGGGTCAGTTAGAGGAGCAGGGTTTTAGTGCTGGGGATGTTAGGCCAGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGGAGGAGAGAAGAAGCAATTGCTAATCTCAGGAAGTGTTTAGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTGGGGAAGGCAAACCGTGATTTGGCTGAAGCTTATGTTGCTATTTTAAACTTCAAGGATGCTCTTCCCTTTTGTATGAAGGCATTGGAGATACATAAGAAGCAGTTAGGACATAATTCAGTTGAAGTTGCCCATGATAGGAGGCTTCTTGGGGTTATCTATAGTGGGTTGGAAGAACATCAAAAGGCTTTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAGTTCCGATTTGATTCGTGCAGAAATTGATGCTGCTAATATGCAAATTGCGCTAGGTAGGTATGATGAGGCCATTAGTACTCTCAAGGATGTTGTTCAGCATACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGGAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTTGACAAGAAAGAAGCAAATTCTCCGGTGGAAGCTGCTGAGGCCTATTCAGAAATATCGATGCAGTATGAGACGATGAATGAGTTTGAAACTGCAATTTCTTTATTAAAAAAGTCTCTAGCATTGCTTGAGAAGCTTCCACAAGAGCAACACTCAGAAGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCTTACTTGGAGGGTGCAGCAGAGACTTTGAAAGAGAGTTTTGGTTCCAAGCATTTTGGAGTTGGGTATATATATAATAATCTTGGGGCTGCGTATTTGGAACTTGAGAGACCTCAATCGGCTGCACAGATGTTTGCAATGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCGGATTCAATTGAAGCATGCCAGAATCTTTCAAAAGCATATGGTGCGATGGGAAGGTATTCTTCACTCTTTTCTCCCTCCCTTGTAACATTGTTACATTCTTTACAACATATAATGTTTTAGTTTTACTTATTATCATTCCTTGGAATCCTTGTTGGGGGAGTTGGGTTTTATGGATCATACAGAATTGGATTAAGGAGATCAAAGTGTTTTGTTTTGAATGGCAGAATAGGAAATTGATCCATGTAATATGTCTTCTAACCTCAAAACAGCTCAATTCATTTTGGGGGAAATTTTAACTTCATTGTTGGGAAATTCAGTTCCCATTGTTTTCTTGTAAACCGAATGCAGAAAGTAGACACGTTTTAATGCTTGGCCTATTTTGCTTAACCATAATCGTTTTCAAATGATCGTGGAAGAATTGGAGGTTAATTAGATTCATTAAGGCCTATTTGATAACTATTTGGTTTTTGGTTTCTACTTTCTAAAAATTATCTTGTTTTCTCATCGATTCTTTATAATGATTTCATCTTTCTTAACAAAATATTTGAATTCTCAGTAAGATTCCAAAAACAAAAACAAGTTTTTGAAAACTATTTTTTTTAGTTTTCAAAACTTGGTTTGGTTTTCGAAAACAAGGTAAAATGTAGATAACAAAGCAAGGAAACCCATAGGTGAAAGTAATCTTTATAGGCTTAATTTTCAAAAACCAAAAATCAAATGATAAAACGATTATCAAACGGGGCCTAAAATGTCAAACAAACTTTGGAGAGGACTCTAACGAATTATTATTGGACTTCCCAAACTGATGGGTTGATCTTGGAATGGAATTTCATTCGAGAGTCACTTGGCCGAAATGATGAGGAAGCAGCACTTGAAAGAAATGATAAATTTCTCTCTAGTATTTCGAATAGAAAATATTACAGGGCAGAGGCCTTTTGTTGGTCTTGCTAAAAAAAATTGGATACGGTGAATATTGAGGTGTAGAGTCTAGGGATATCCTGATATGAAATAACTAAGTCGTCTTCGGAGATGACTGCTTCCGAACAGACTTGATACCATGAGTTGGATGTACGGGCTGAATAGATTTTAAATGACTTTTTAACTGTCTGAAGGTATTAGACACTGTCTTCAGTTCTGCCTCTTTCTTATTGCCCTTAGCGTTTTTTTCATTTGTTATTGAATGATGCAAAAACCTTGCAAAAATTCTGTTTTTCTATGGATATTTATGATGAAAACCTTTGTTGTTTGCACCCATGGATCATTAGAGTTACTCAGCAGTAAGTTGGCTATTTAGGGTCATTCTTGAACTTATTAATTTACTCCTTTTTCTATGTGTTGGTGCAGTTATGCCCTTGCAATTGAATTCCAAGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCAACTGCCGAAGATGAACTTAGAGAAGCCCAACGAATGCTCGAACAATTAAAGAGGAAAGCACATGGTACATCCAAAATCGAGCTCTCGACGAAGGCCTTGCCCCTACCTCATTCCCCGACGTCTTCCAAAACTTTGCAGCCTGATACTCCCAGCAGTCAGAATCATCTATAGCCACAACACAGTAAATCTGATAATCTATTTACAACTCCAGAAAAATCAATAGATCAGATCAACACTTCTTGGTAAGTTTTATCCTCAAAAAGCCCTGATAGTTACGGAAATATGTTTCTTTAAAACTTTGGTCATCTTGTGCATAGCTGGAACAACCTTGAAATATAAGATCCCATCCTTTGCATGCATGTCTATCTATCCTGCTAAGAAACGTATTTACTGAAAGCTATAATCCGGGGAAAGAGACGATTGCAGTAGCCCGTAGAATTATCGAATCCGATGGCTGTGTTTTCTGAAACCATCTCACTGATTCTATGTTCGCAAATTTGGACGATGCTCACGACAAATGATTTTGATTCTCAAGATTTTCTTTTGTTTGAACTATCATAACGATGAGTGCTGAGATAATAAGTTTATAGATGTTTGTTTTACTTTTACACTGGTGTTTCAGAATTACTTGTATTGTAAATGAGTTTGTAGTTTGGTTTTAGTTGCATAACTTCCAAGATATCACTTTCTGGGTTGCCTAGAATCAATGAGATGTTATAATGGAAGTACTTGTTTTAAACTTGATGGTTTTGTAGATTGTTATTGATGGA

mRNA sequence

GTTTTGCAAAGGTTTAGGCTGAAACAACCAAAAACCTCGCAAAAGCGCAGACTATTCACTATCACTTTCAATCCCACAATGAGAAGAAACGCTTCGATTTCACTTCTCTCTCACTCCGATCGTCTCAATCTCCGCTCACTTTCATCCCTCATTTCCCCAAATTCCATTTCCAATCCTTCCCCTTCGTATTCCTCCCCTGTTTCAACCCCCCATCACAAGCCATGCACCAAAAACCATGGTTTGTTCTTCAAATTCACCCGATTTCAAGCAAGCCCATCTCGAAATTTGGATACCCTTGTGGAGAAACGCCCTCAAATTTCCTCCAGACAGCGCAAAGTCAGAGAGAAATCCGAGCTTGAGGAGGCTTTTGAGTCTGCAAACACTGTTGATGAAATGCTCAAGGCGTTTAAGGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGACCCTGAAAAGGCTCTGTTGTACGCTGGTAGAGCTTTGAATGCTTTTGATAAAGATGATAATGGGAAACCTTCTTTACTTGTTGCTATGACTTTACAATTGATGGGTTCTGTTAATTATAGCTTGAAAAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCTAATAGGATATTGGGTCAGTTAGAGGAGCAGGGTTTTAGTGCTGGGGATGTTAGGCCAGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGGAGGAGAGAAGAAGCAATTGCTAATCTCAGGAAGTGTTTAGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTGGGGAAGGCAAACCGTGATTTGGCTGAAGCTTATGTTGCTATTTTAAACTTCAAGGATGCTCTTCCCTTTTGTATGAAGGCATTGGAGATACATAAGAAGCAGTTAGGACATAATTCAGTTGAAGTTGCCCATGATAGGAGGCTTCTTGGGGTTATCTATAGTGGGTTGGAAGAACATCAAAAGGCTTTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAGTTCCGATTTGATTCGTGCAGAAATTGATGCTGCTAATATGCAAATTGCGCTAGGTAGGTATGATGAGGCCATTAGTACTCTCAAGGATGTTGTTCAGCATACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGGAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTTGACAAGAAAGAAGCAAATTCTCCGGTGGAAGCTGCTGAGGCCTATTCAGAAATATCGATGCAGTATGAGACGATGAATGAGTTTGAAACTGCAATTTCTTTATTAAAAAAGTCTCTAGCATTGCTTGAGAAGCTTCCACAAGAGCAACACTCAGAAGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCTTACTTGGAGGGTGCAGCAGAGACTTTGAAAGAGAGTTTTGGTTCCAAGCATTTTGGAGTTGGGTATATATATAATAATCTTGGGGCTGCGTATTTGGAACTTGAGAGACCTCAATCGGCTGCACAGATGTTTGCAATGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCGGATTCAATTGAAGCATGCCAGAATCTTTCAAAAGCATATGGTGCGATGGGAAGTTATGCCCTTGCAATTGAATTCCAAGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCAACTGCCGAAGATGAACTTAGAGAAGCCCAACGAATGCTCGAACAATTAAAGAGGAAAGCACATGGTACATCCAAAATCGAGCTCTCGACGAAGGCCTTGCCCCTACCTCATTCCCCGACGTCTTCCAAAACTTTGCAGCCTGATACTCCCAGCAGTCAGAATCATCTATAGCCACAACACAGTAAATCTGATAATCTATTTACAACTCCAGAAAAATCAATAGATCAGATCAACACTTCTTGGTAAGTTTTATCCTCAAAAAGCCCTGATAGTTACGGAAATATGTTTCTTTAAAACTTTGGTCATCTTGTGCATAGCTGGAACAACCTTGAAATATAAGATCCCATCCTTTGCATGCATGTCTATCTATCCTGCTAAGAAACGTATTTACTGAAAGCTATAATCCGGGGAAAGAGACGATTGCAGTAGCCCGTAGAATTATCGAATCCGATGGCTGTGTTTTCTGAAACCATCTCACTGATTCTATGTTCGCAAATTTGGACGATGCTCACGACAAATGATTTTGATTCTCAAGATTTTCTTTTGTTTGAACTATCATAACGATGAGTGCTGAGATAATAAGTTTATAGATGTTTGTTTTACTTTTACACTGGTGTTTCAGAATTACTTGTATTGTAAATGAGTTTGTAGTTTGGTTTTAGTTGCATAACTTCCAAGATATCACTTTCTGGGTTGCCTAGAATCAATGAGATGTTATAATGGAAGTACTTGTTTTAAACTTGATGGTTTTGTAGATTGTTATTGATGGA

Coding sequence (CDS)

ATGAGAAGAAACGCTTCGATTTCACTTCTCTCTCACTCCGATCGTCTCAATCTCCGCTCACTTTCATCCCTCATTTCCCCAAATTCCATTTCCAATCCTTCCCCTTCGTATTCCTCCCCTGTTTCAACCCCCCATCACAAGCCATGCACCAAAAACCATGGTTTGTTCTTCAAATTCACCCGATTTCAAGCAAGCCCATCTCGAAATTTGGATACCCTTGTGGAGAAACGCCCTCAAATTTCCTCCAGACAGCGCAAAGTCAGAGAGAAATCCGAGCTTGAGGAGGCTTTTGAGTCTGCAAACACTGTTGATGAAATGCTCAAGGCGTTTAAGGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGACCCTGAAAAGGCTCTGTTGTACGCTGGTAGAGCTTTGAATGCTTTTGATAAAGATGATAATGGGAAACCTTCTTTACTTGTTGCTATGACTTTACAATTGATGGGTTCTGTTAATTATAGCTTGAAAAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCTAATAGGATATTGGGTCAGTTAGAGGAGCAGGGTTTTAGTGCTGGGGATGTTAGGCCAGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGGAGGAGAGAAGAAGCAATTGCTAATCTCAGGAAGTGTTTAGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTGGGGAAGGCAAACCGTGATTTGGCTGAAGCTTATGTTGCTATTTTAAACTTCAAGGATGCTCTTCCCTTTTGTATGAAGGCATTGGAGATACATAAGAAGCAGTTAGGACATAATTCAGTTGAAGTTGCCCATGATAGGAGGCTTCTTGGGGTTATCTATAGTGGGTTGGAAGAACATCAAAAGGCTTTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAGTTCCGATTTGATTCGTGCAGAAATTGATGCTGCTAATATGCAAATTGCGCTAGGTAGGTATGATGAGGCCATTAGTACTCTCAAGGATGTTGTTCAGCATACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGGAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTTGACAAGAAAGAAGCAAATTCTCCGGTGGAAGCTGCTGAGGCCTATTCAGAAATATCGATGCAGTATGAGACGATGAATGAGTTTGAAACTGCAATTTCTTTATTAAAAAAGTCTCTAGCATTGCTTGAGAAGCTTCCACAAGAGCAACACTCAGAAGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCTTACTTGGAGGGTGCAGCAGAGACTTTGAAAGAGAGTTTTGGTTCCAAGCATTTTGGAGTTGGGTATATATATAATAATCTTGGGGCTGCGTATTTGGAACTTGAGAGACCTCAATCGGCTGCACAGATGTTTGCAATGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCGGATTCAATTGAAGCATGCCAGAATCTTTCAAAAGCATATGGTGCGATGGGAAGTTATGCCCTTGCAATTGAATTCCAAGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCAACTGCCGAAGATGAACTTAGAGAAGCCCAACGAATGCTCGAACAATTAAAGAGGAAAGCACATGGTACATCCAAAATCGAGCTCTCGACGAAGGCCTTGCCCCTACCTCATTCCCCGACGTCTTCCAAAACTTTGCAGCCTGATACTCCCAGCAGTCAGAATCATCTATAG

Protein sequence

MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFTRFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSLKRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAISTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAHGTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNHL
Homology
BLAST of Tan0002450 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.5e-46
Identity = 163/578 (28.20%), Postives = 270/578 (46.71%), Query Frame = 0

Query: 38  SSPVSTPHHKPCTKNHGLFFKFTRFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAF 97
           + P+S P   P  K        T  ++ PS N  T  +  P +SS    V +        
Sbjct: 26  TQPLSNPPRTPMKKTP----SSTPSRSKPSPNRSTGKKDSPTVSSSTAAVID-------- 85

Query: 98  ESANTVDEMLKAFKDMESAFEERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDK- 157
                VD         + + +  DLG   LK+       GE P KAL YA RA  +F++ 
Sbjct: 86  -----VD---------DPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSFERC 145

Query: 158 ----------DDNGKPSLLVAMTLQLMGSVNYSLKRFSDSLGYLNRANRILGQLEEQGFS 217
                       +G P L +AM+L ++ ++  SL RF +++  L RA ++         S
Sbjct: 146 CAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHS 205

Query: 218 AGDVRPVLHAVQLELANVKTAMGRREEAIANLRKCLEIKELTLEKDSRELGKANRDLAEA 277
                    +  ++L +  + +G+ + +IA   + L+I+  TL      +G+  R LAEA
Sbjct: 206 L-----AAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEA 265

Query: 278 YVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQ 337
           YV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   L+ 
Sbjct: 266 YVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLVLAS 325

Query: 338 KVLKNWGLSSDLIRAEIDAANMQIALGRYDEAI-STLKDVVQHTDKDSETR---AVVFIS 397
             +   G  S++   ++   N+ ++L R+DEA+ S  K +        ET    A VF+ 
Sbjct: 326 MAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVR 385

Query: 398 MGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPV-EAAEAYSEISMQYETMNEFETAISL 457
           + ++     K  ++K   E A  I++K    + V E A   +EIS  YE+++E E A+ L
Sbjct: 386 LAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKL 445

Query: 458 LKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGV 517
           L+KS+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ + G K    
Sbjct: 446 LQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKSAFF 505

Query: 518 GYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYA 577
           G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+  Y AMG   
Sbjct: 506 GVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIE 565

Query: 578 LAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKA 600
            AIE  EQV+   E    TA  +  + ++ L +L ++A
Sbjct: 566 DAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of Tan0002450 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 1.3e-45
Identity = 144/497 (28.97%), Postives = 240/497 (48.29%), Query Frame = 0

Query: 118 EERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKP--SLLVAMTLQLMGS 177
           E  +LG+  LK   +L   GE+  KAL  A RA+  F+K   G+    L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 178 VNYSLKRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAI 237
           +   L R++D++  L R+  I   + E G    D      A  ++L ++   MG+ E +I
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI--PMIEDG---EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 238 ANLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQ--LGH 297
                 LEI+   L +    +G+  R LAEA+V  + F++A   C  AL+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 298 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALG 357
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 358 RYDEAISTLKDVV----QHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 417
           R+DEAI   +  +    Q   +   + A+V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 418 KEANSPV-EAAEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGW 477
               +P+ E A  + EIS  Y++MNE + A+ LL+++L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 478 LLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMA 537
           +  + G   E+    + A    + S   K    G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 538 KDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELR-E 597
           K I++   GP+H D++    NL+  Y AMG    AIE  E VV   E    TA  E+  E
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 598 AQRMLEQLKRKAHGTSK 605
            QR+   LK    G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Tan0002450 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 8.1e-40
Identity = 155/567 (27.34%), Postives = 257/567 (45.33%), Query Frame = 0

Query: 65  SPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEERDLGL 124
           S S NLD  V  R       +K   KS +         V        + E + E  +L  
Sbjct: 89  SNSSNLDLEVMPRDMEKQTGKKNVTKSNVGVGGMRKKKVGGTKLQNGNEEPSSENVELAR 148

Query: 125 ASLKIGLKLDQEGEDPEKALLYAGRALNAFDKD-DNGKPSLLVAMTLQLMGSVNYSLKRF 184
             L     L   G+   KAL    RA   F+   +NGKP L   M L +  +V+  LK +
Sbjct: 149 FLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHVTAAVHCKLKEY 208

Query: 185 SDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRKCLE 244
           ++++  L R+  I   + E+G      +    A  ++L +    +G+ E +I+   + L 
Sbjct: 209 NEAIPVLQRSVEI--PVVEEGEEHALAK---FAGLMQLGDTYAMVGQLESSISCYTEGLN 268

Query: 245 IKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRR 304
           I++  L ++   +G+  R LAEA V  L F +A   C  AL IH++     S+  A DRR
Sbjct: 269 IQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGLPGSIAEAADRR 328

Query: 305 LLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAISTLK 364
           L+G+I     +H+ ALE   L+   +   G  S++   +    +  ++L R+DEAI   +
Sbjct: 329 LMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSLSRFDEAICAYQ 388

Query: 365 D--VVQHTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEAN-SPVEA 424
                  T K     AV  V+I +  +     K  +AK   E A  I++      SP E 
Sbjct: 389 KSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYESHNLEISPEEI 448

Query: 425 AEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQE 484
           A   ++IS+  E+MNE E AI+LL+K+L +    P ++     + A++G L  + GK  E
Sbjct: 449 ASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMGVLYYMMGKYME 508

Query: 485 AIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGP 544
           +    + A   L+ +   +    G   N +G A ++L+  + A ++F  AK I++   GP
Sbjct: 509 SYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEEAKCILEQECGP 568

Query: 545 HHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRK 604
           +H +++    NL+ AY A+G    AI+    VV   E    TA     + +R L QL ++
Sbjct: 569 YHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTEDEKRRLAQLLKE 628

Query: 605 AHGTSKIELSTKALPLPHSPTSSKTLQ 626
           A   +  +  +    +    TSS  L+
Sbjct: 629 AGNVTGRKAKSLKTLIDSDLTSSSALR 650

BLAST of Tan0002450 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 1.1e-15
Identity = 83/355 (23.38%), Postives = 155/355 (43.66%), Query Frame = 0

Query: 228  MGRREEAIANLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIH 287
            +G   +A+  L++ LEI+E  L+ D   + ++   LA  YV    F +A     +ALEI 
Sbjct: 940  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYVQSKKFGNAEQLYKQALEIS 999

Query: 288  KKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAAN 347
            +   G   + VA +   L V+Y    + ++A +  + S K+ +          A    + 
Sbjct: 1000 ENAYGSEHMRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1059

Query: 348  MQIALGRYDEAISTLKDVVQHTDKDSETRAVVFISMGKVLCN--QEKFTDAKRCLEIASG 407
               AL R       L+++    D     R +  + +   L N  +   T  KR LE+   
Sbjct: 1060 YGFALLR--RRALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMRER 1119

Query: 408  IFDKKEANSPVEAAEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSAR 467
            +     A+ P + A++ + ++  Y    +++ A  L +++L +  +     H   + + +
Sbjct: 1120 VLG---ADHP-DCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVK 1179

Query: 468  -LGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQM 527
             L  L    GK+ +A+P  E A E  ++SFG KH  V     NL   Y ++++   A+ +
Sbjct: 1180 HLAVLYKRKGKLDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPL 1239

Query: 528  FAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGS 580
            +  A  I + SLG  H    E  +NL+      G +  A E  ++ ++  E+  S
Sbjct: 1240 YERAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDFEKAAELYKRAMEIKETETS 1280

BLAST of Tan0002450 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.4e-15
Identity = 82/355 (23.10%), Postives = 154/355 (43.38%), Query Frame = 0

Query: 228  MGRREEAIANLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIH 287
            +G   +A+  L++ LEI+E  L+ D   + ++   LA  Y+    F +A     +ALEI 
Sbjct: 929  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYMQSKKFGNAEQLYKQALEIS 988

Query: 288  KKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAAN 347
            +   G   + VA +   L V+Y    + ++A +  + S K+ +          A    + 
Sbjct: 989  ENAYGSEHLRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1048

Query: 348  MQIALGRYDEAISTLKDVVQHTDKDSETRAVVFISMGKVLCN--QEKFTDAKRCLEIASG 407
               AL R       L+++    D     R +  + +   L N  +   T  KR LE+   
Sbjct: 1049 YGFALLR--RRALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMRER 1108

Query: 408  IFDKKEANSPVEAAEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSAR 467
            +     A+ P + A++ + ++  Y    +++ A  L +++L +  +     H   + + +
Sbjct: 1109 VLG---ADHP-DCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVK 1168

Query: 468  -LGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQM 527
             L  L    GK+ +A+P  E A +  ++SFG KH  V     NL   Y ++++   A  +
Sbjct: 1169 HLAVLYKRKGKLDKAVPLYELAVDIRQKSFGPKHPSVATALVNLAVLYCQMKKQDDALPL 1228

Query: 528  FAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGS 580
            +  A  I + SLG  H    E  +NL+      G Y  A E  ++ ++  E+  S
Sbjct: 1229 YERAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDYEKAAELYKRAMEIKETETS 1269

BLAST of Tan0002450 vs. NCBI nr
Match: XP_038899312.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 590/632 (93.35%), Postives = 608/632 (96.20%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLLSHS+RLNLRSLSS IS NSISNPSPS SSPVSTP+ KPCTKNHGLFFKFT
Sbjct: 1   MRRNASISLLSHSNRLNLRSLSSFISSNSISNPSPSNSSPVSTPNLKPCTKNHGLFFKFT 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNLDTLVEKRPQISSRQRKV+EKSELEEAFESA+TVDEM+KAFKDMESAFEER
Sbjct: 61  RFQANPSRNLDTLVEKRPQISSRQRKVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE  LLYA RAL A DKD NG PSL VAMTLQLMGSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPENILLYANRALKALDKDVNGNPSLPVAMTLQLMGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQL E+GFSA DVRPVLHAVQLELANVKTAMGRREEA++NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLVEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELG ANRDLAEAYVA+LNFKDALPFCMKALEIHK QLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGNANRDLAEAYVAVLNFKDALPFCMKALEIHKMQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLL VIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGRY+EAIS
Sbjct: 301 DRRLLAVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYNEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTD KRCLEIASGIFDK+EA SPVEAAE
Sbjct: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDPKRCLEIASGIFDKREATSPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYETMNEFETAISLLKKSLALLEKLP EQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPHEQHSEGSVSARLGWLLLLTGKVQEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC+NLSKAYGAMGSY LAIEFQEQVVDAWESHGSTAEDELREAQ+ML+QLKRKAH
Sbjct: 541 ADSIEACRNLSKAYGAMGSYPLAIEFQEQVVDAWESHGSTAEDELREAQQMLKQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQ 633
           GTSKIELSTKALPLPHS TSSKTLQPD P+ Q
Sbjct: 601 GTSKIELSTKALPLPHSSTSSKTLQPDLPARQ 632

BLAST of Tan0002450 vs. NCBI nr
Match: XP_023549125.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 582/634 (91.80%), Postives = 604/634 (95.27%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL HS+R+NLRSLSSLISP  IS+PSPSYSSPVS PH  PCTKNHGLFFKF 
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKFN 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQASPSRNL+TLVEKRPQISSRQRK +EKSELEEAFESANTVDEM+KAFKDMES FEER
Sbjct: 61  RFQASPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL A DKDDNGKPSL VAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSA  +RPVLHAVQLELANVKTAMGRREEA+ANLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPV AAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVAAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGSTAEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSTAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           G SKIELS KALPLPHS TSSKTLQPD PSSQ H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Tan0002450 vs. NCBI nr
Match: XP_022954096.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata])

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 579/634 (91.32%), Postives = 603/634 (95.11%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL HS+R+NLRSLSSLISP  IS+PSPSYSSPVS PH  PCTKNHGLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQRK +EKSELEEAFESANTVDEM+KAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL A DKDDNGKPSL VAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSA  +RPVLHAVQLELANVKTAMGRREEA+ANLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           G SKIELS KALPLPHS TSSKTLQPD PSSQ H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Tan0002450 vs. NCBI nr
Match: KAG7014584.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 576/634 (90.85%), Postives = 603/634 (95.11%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL HS+R+NLRSLSSLISP  IS+PSPSYSSPVS  H  PCTKNHGLFFKF+
Sbjct: 11  MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSASHLNPCTKNHGLFFKFS 70

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQRK +EKSELEEAFESANTVDEM+KAFKDMES FEER
Sbjct: 71  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 130

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL A DKDDNGKPSL VAMTLQL+GSVNYSL
Sbjct: 131 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 190

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSA  +RPVLHAVQLELANVKTAMGRREEA+A+LRK
Sbjct: 191 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALASLRK 250

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSREL KANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 251 CLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 310

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGR+DEAIS
Sbjct: 311 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDEAIS 370

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA+SPVEAAE
Sbjct: 371 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVEAAE 430

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 431 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 490

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 491 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 550

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++ SYALAIEFQEQVVDAWE HGS AEDELREAQRMLEQLKRKAH
Sbjct: 551 ADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 610

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           G SKIELS KALPLPHS TSSKTLQPD PSSQ H
Sbjct: 611 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 644

BLAST of Tan0002450 vs. NCBI nr
Match: KAG6576067.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 576/634 (90.85%), Postives = 603/634 (95.11%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL HS+R+NLRSLSSLISP  IS+PSPSYSSPVS  H  PCTKNHGLFFKF+
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSASHLNPCTKNHGLFFKFS 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQRK +EKSELEEAFESANTVDEM+KAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL A DKDDNGKPSL VAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSA  +RPVLHAVQLELANVKTAMGRREEA+A+LRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALASLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSREL KANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGR+DEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA+SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++ SYALAIEFQEQVVDAWE HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           G SKIELS KALPLPHS TSSKTLQPD PSSQ H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Tan0002450 vs. ExPASy TrEMBL
Match: A0A6J1GPX7 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456463 PE=4 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 579/634 (91.32%), Postives = 603/634 (95.11%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL HS+R+NLRSLSSLISP  IS+PSPSYSSPVS PH  PCTKNHGLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQRK +EKSELEEAFESANTVDEM+KAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL A DKDDNGKPSL VAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSA  +RPVLHAVQLELANVKTAMGRREEA+ANLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           G SKIELS KALPLPHS TSSKTLQPD PSSQ H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKH 634

BLAST of Tan0002450 vs. ExPASy TrEMBL
Match: A0A6J1JWL0 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC111488134 PE=4 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 576/634 (90.85%), Postives = 598/634 (94.32%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL HS+R NLRSLSSLISP  IS+PS SYSSPVST H  PCTKNHGLFFKF 
Sbjct: 1   MRRNASISLLFHSNRFNLRSLSSLISPKFISDPSFSYSSPVSTTHLNPCTKNHGLFFKFK 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQRK +EKSELEEAFESANTVDEM+KAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL A DKDDNGK SL VAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKLSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSA  +RPVLHAVQLELANVKTAMGRREEA+ANLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKA RDLAEAYVAILNFKDAL FCM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKAKRDLAEAYVAILNFKDALLFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGL+SDL+RAEIDAANMQIALGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEFETAISLLK+SLALLEKLPQEQHS GSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSVGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           G SKIELS KALPLPHS TSSKTLQPD PSSQ H
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQEH 634

BLAST of Tan0002450 vs. ExPASy TrEMBL
Match: A0A6J1CRC1 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Momordica charantia OX=3673 GN=LOC111013468 PE=4 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 571/635 (89.92%), Postives = 601/635 (94.65%), Query Frame = 0

Query: 3   RNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFTRF 62
           R ASISLLSHS+RLNLRS+SSLIS +   +P PS SSP+S  H  PC+K+HGLFFK TRF
Sbjct: 2   RKASISLLSHSNRLNLRSISSLIS-DPPPSPPPS-SSPLSAAHLNPCSKSHGLFFKLTRF 61

Query: 63  QASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEERDL 122
           QA+P RNLDTLVEKRPQISSRQRK++EKSELEEAFESANTVDEML AFKDMESAFEERDL
Sbjct: 62  QANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDL 121

Query: 123 GLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDN-GKPSLLVAMTLQLMGSVNYSLK 182
           GLASLKIGLKLDQEGEDPEK LLYA RAL A D DDN GKPSLLVAMTLQLMGSVNYSLK
Sbjct: 122 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLK 181

Query: 183 RFSDSLGYLNRANRILGQLEEQGFSAG-DVRPVLHAVQLELANVKTAMGRREEAIANLRK 242
           +FSDSLGYLNRANR+L QLEE+GFSA  D+RPVLHAVQLELANVKTAMGRREEA+ NLRK
Sbjct: 182 KFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRK 241

Query: 243 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 302
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNF+DALPFCMKALEIHK QLGHNSVEVAH
Sbjct: 242 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAH 301

Query: 303 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 362
           DRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGL+SDL+ AEIDAANMQIALGRYDEAIS
Sbjct: 302 DRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIS 361

Query: 363 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 422
           TLKDV Q TDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEA SPVEAAE
Sbjct: 362 TLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 421

Query: 423 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 482
           AYSEISMQYETMNEFETAISLLK+SLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKVQEAI
Sbjct: 422 AYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAI 481

Query: 483 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 542
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 482 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 541

Query: 543 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 602
           ADSIE+CQNLSKAYGAMGSYALAI+FQEQVVDAWESHGS+AEDEL+EAQRMLEQLKRKAH
Sbjct: 542 ADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAH 601

Query: 603 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNHL 636
           GTSK ELSTKALPLPHSPTSSK+LQP+ P SQ  L
Sbjct: 602 GTSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL 634

BLAST of Tan0002450 vs. ExPASy TrEMBL
Match: A0A0A0K8P3 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 PE=4 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 2.6e-307
Identity = 559/634 (88.17%), Postives = 593/634 (93.53%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASISLL + +RLNLRSLSSLISPNSISN  PS SSPVSTP+ KPCT N+GLFFKFT
Sbjct: 12  MRRNASISLLPYFNRLNLRSLSSLISPNSISN-LPSNSSPVSTPNLKPCTINNGLFFKFT 71

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+PSRNLDTLVEKRP+ISSRQRK+++KS LEE+FESA TVDEM KAFK+MESAFEE+
Sbjct: 72  RFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEK 131

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKI LKLD+EGEDPE+ LLYA RAL A DKDDN  PSL VAMTLQL+GSVNYSL
Sbjct: 132 DLGLASLKIALKLDREGEDPEQVLLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 191

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE+G+SA D+RPVLHAV LELANVKTAMGRREEA++NLRK
Sbjct: 192 KRFSDSLGYLNRANRILGQLEEKGYSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRK 251

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
           CLEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCMK LEIHKK+LG+NSVEVAH
Sbjct: 252 CLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 311

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEE+QKALEQNELS+KVLKNWGL+SDL+RAE DAANMQIALGRYDEAI+
Sbjct: 312 DRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAIN 371

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDV QHTDKDSE R VVF SMGKVLCNQEKFTDAKRCLEIASGIFDK+E  SPVEAAE
Sbjct: 372 TLKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAE 431

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYETMNEF+TAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 432 AYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 491

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGS HFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 492 PYLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 551

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQEQVVDAW+SHGS+AEDELREA+R LEQLKRKA 
Sbjct: 552 ADSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAR 611

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           GTSK E STKALPLP S TSSKTLQPD P+ Q H
Sbjct: 612 GTSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQH 644

BLAST of Tan0002450 vs. ExPASy TrEMBL
Match: A0A1S3BR15 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 3.4e-307
Identity = 559/634 (88.17%), Postives = 595/634 (93.85%), Query Frame = 0

Query: 1   MRRNASISLLSHSDRLNLRSLSSLISPNSISNPSPSYSSPVSTPHHKPCTKNHGLFFKFT 60
           MRRNASIS+L + +RLNLRSLSSLISPNSISN  P  SSPVSTP+ KPCTKN+GLFFKFT
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISN-LPLNSSPVSTPNLKPCTKNNGLFFKFT 60

Query: 61  RFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEER 120
           RFQA+ SRNLDTLVEKRP++SSRQRK++EKS+LEEAFESA+TVDEM+KAFK+MESAFEE+
Sbjct: 61  RFQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEK 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE+ LLYA RAL A DKDDN  PSL VAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRK 240
           KRFSDSLGYLNRANRILGQLEE+GFSA DVRPVLHAVQLELANVKTAMGRREEA++NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAH 300
            LEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCMK LEIHKK+LG+NSVEVAH
Sbjct: 241 SLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELS+KVLKNWGL+SDL+RAEI+AANMQIALGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPVEAAE 420
           TLKDVVQHTDKDSE R VVF SMGKVLCNQEKFTD KRCLEIA GIFDK+E  SP  AAE
Sbjct: 361 TLKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAE 420

Query: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISM YETMN+FETAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAI
Sbjct: 421 AYSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
           PYLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREA++ L+QLKRKA 
Sbjct: 541 ADSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQ 600

Query: 601 GTSKIELSTKALPLPHSPTSSKTLQPDTPSSQNH 635
           GTSK ELSTKALPLPHS TSSKTLQPD P+ Q H
Sbjct: 601 GTSKFELSTKALPLPHSSTSSKTLQPDLPAHQKH 633

BLAST of Tan0002450 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 760.8 bits (1963), Expect = 9.0e-220
Identity = 411/626 (65.65%), Postives = 502/626 (80.19%), Query Frame = 0

Query: 3   RNASISLLS--HSDRLNLRSLSSLISPNSISNPSPSYSSP---VSTPHHKPCTKNHGLFF 62
           ++ SISL+   H  ++    LS  ++  S S+ S S  SP   +ST     C + + L  
Sbjct: 2   KSLSISLIRRLHFHKIRPIPLSIHLNAASSSSSSSSLISPAREIST----SCRRTNDLIK 61

Query: 63  KFTRFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAF 122
           +  +F  +P RNL+T VE+ P+ISS     +EK +LEEAFESANT DEM++ FK+ME +F
Sbjct: 62  R--QFWVNPYRNLNTHVEEPPEISSSD---KEKIDLEEAFESANTTDEMVRLFKEMELSF 121

Query: 123 EERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKPSLLVAMTLQLMGSVN 182
           E  +LGL++LK+GL LD+EGEDPEK L YA +AL +FD D N KP+LLVAM  QLMGS N
Sbjct: 122 EGNELGLSALKLGLHLDREGEDPEKVLSYADKALKSFDGDGN-KPNLLVAMASQLMGSAN 181

Query: 183 YSLKRFSDSLGYLNRANRILGQLEEQG-FSAGDVRPVLHAVQLELANVKTAMGRREEAIA 242
           Y LKRFSDSLGYLNRANRIL +LE  G     DVRPVLHAVQLELANVK AMGRREEAI 
Sbjct: 182 YGLKRFSDSLGYLNRANRILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIE 241

Query: 243 NLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSV 302
           NL+K LEIKE+T ++DS+E+G ANR LA+AYVA+LNF +ALP+ +KALEIHKK+LG+NS 
Sbjct: 242 NLKKSLEIKEMTFDEDSKEMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSA 301

Query: 303 EVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYD 362
           EVA DRRLLGVIYSGLE+H KALEQN LSQ+VLKNWG+  +LIRAEIDAANM++ALG+Y+
Sbjct: 302 EVAQDRRLLGVIYSGLEQHDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYE 361

Query: 363 EAISTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPV 422
           EAI  LK VVQ TDKDSE RA+VFISM K L NQ+KF ++KRCLE A  I +KKE   PV
Sbjct: 362 EAIDILKSVVQQTDKDSEMRAMVFISMSKALVNQQKFAESKRCLEFACEILEKKETALPV 421

Query: 423 EAAEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV 482
           E AEAYSE++MQYE+MNEFETAISLL+K+L +LEKLPQEQHSEGSVSAR+GWLLL +G+V
Sbjct: 422 EVAEAYSEVAMQYESMNEFETAISLLQKTLGILEKLPQEQHSEGSVSARIGWLLLFSGRV 481

Query: 483 QEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSL 542
            +A+PYLE AAE LKESFG+KHFGVGY+YNNLGAAYLEL RPQSAAQMFA+AKDIMDVSL
Sbjct: 482 SQAVPYLESAAERLKESFGAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDVSL 541

Query: 543 GPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLK 602
           GP+H DSI+ACQNLSKAY  MG+Y+LA+EFQ++V++AW++HG +A+DE++EA+R+LE L+
Sbjct: 542 GPNHVDSIDACQNLSKAYAGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLEDLR 601

Query: 603 RKAH-GTSKIELSTKALPLPHSPTSS 622
            KA  G S  +L  KALPLP    SS
Sbjct: 602 LKARGGVSTNKLLNKALPLPKPSHSS 617

BLAST of Tan0002450 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 1.1e-47
Identity = 163/578 (28.20%), Postives = 270/578 (46.71%), Query Frame = 0

Query: 38  SSPVSTPHHKPCTKNHGLFFKFTRFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAF 97
           + P+S P   P  K        T  ++ PS N  T  +  P +SS    V +        
Sbjct: 26  TQPLSNPPRTPMKKTP----SSTPSRSKPSPNRSTGKKDSPTVSSSTAAVID-------- 85

Query: 98  ESANTVDEMLKAFKDMESAFEERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDK- 157
                VD         + + +  DLG   LK+       GE P KAL YA RA  +F++ 
Sbjct: 86  -----VD---------DPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSFERC 145

Query: 158 ----------DDNGKPSLLVAMTLQLMGSVNYSLKRFSDSLGYLNRANRILGQLEEQGFS 217
                       +G P L +AM+L ++ ++  SL RF +++  L RA ++         S
Sbjct: 146 CAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHS 205

Query: 218 AGDVRPVLHAVQLELANVKTAMGRREEAIANLRKCLEIKELTLEKDSRELGKANRDLAEA 277
                    +  ++L +  + +G+ + +IA   + L+I+  TL      +G+  R LAEA
Sbjct: 206 L-----AAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEA 265

Query: 278 YVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQ 337
           YV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   L+ 
Sbjct: 266 YVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLVLAS 325

Query: 338 KVLKNWGLSSDLIRAEIDAANMQIALGRYDEAI-STLKDVVQHTDKDSETR---AVVFIS 397
             +   G  S++   ++   N+ ++L R+DEA+ S  K +        ET    A VF+ 
Sbjct: 326 MAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVR 385

Query: 398 MGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPV-EAAEAYSEISMQYETMNEFETAISL 457
           + ++     K  ++K   E A  I++K    + V E A   +EIS  YE+++E E A+ L
Sbjct: 386 LAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKL 445

Query: 458 LKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGV 517
           L+KS+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ + G K    
Sbjct: 446 LQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKSAFF 505

Query: 518 GYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYA 577
           G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+  Y AMG   
Sbjct: 506 GVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIE 565

Query: 578 LAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRKA 600
            AIE  EQV+   E    TA  +  + ++ L +L ++A
Sbjct: 566 DAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of Tan0002450 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 9.1e-47
Identity = 144/497 (28.97%), Postives = 240/497 (48.29%), Query Frame = 0

Query: 118 EERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDKDDNGKP--SLLVAMTLQLMGS 177
           E  +LG+  LK   +L   GE+  KAL  A RA+  F+K   G+    L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 178 VNYSLKRFSDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAI 237
           +   L R++D++  L R+  I   + E G    D      A  ++L ++   MG+ E +I
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI--PMIEDG---EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 238 ANLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQ--LGH 297
                 LEI+   L +    +G+  R LAEA+V  + F++A   C  AL+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 298 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALG 357
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 358 RYDEAISTLKDVV----QHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 417
           R+DEAI   +  +    Q   +   + A+V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 418 KEANSPV-EAAEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGW 477
               +P+ E A  + EIS  Y++MNE + A+ LL+++L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 478 LLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMA 537
           +  + G   E+    + A    + S   K    G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 538 KDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELR-E 597
           K I++   GP+H D++    NL+  Y AMG    AIE  E VV   E    TA  E+  E
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 598 AQRMLEQLKRKAHGTSK 605
            QR+   LK    G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Tan0002450 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 7.2e-44
Identity = 151/537 (28.12%), Postives = 250/537 (46.55%), Query Frame = 0

Query: 38  SSPVSTPHHKPCTKNHGLFFKFTRFQASPSRNLDTLVEKRPQISSRQRKVREKSELEEAF 97
           + P+S P   P  K        T  ++ PS N  T  +  P +SS    V +        
Sbjct: 26  TQPLSNPPRTPMKKTP----SSTPSRSKPSPNRSTGKKDSPTVSSSTAAVID-------- 85

Query: 98  ESANTVDEMLKAFKDMESAFEERDLGLASLKIGLKLDQEGEDPEKALLYAGRALNAFDK- 157
                VD         + + +  DLG   LK+       GE P KAL YA RA  +F++ 
Sbjct: 86  -----VD---------DPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSFERC 145

Query: 158 ----------DDNGKPSLLVAMTLQLMGSVNYSLKRFSDSLGYLNRANRILGQLEEQGFS 217
                       +G P L +AM+L ++ ++  SL RF +++  L RA ++         S
Sbjct: 146 CAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHS 205

Query: 218 AGDVRPVLHAVQLELANVKTAMGRREEAIANLRKCLEIKELTLEKDSRELGKANRDLAEA 277
                    +  ++L +  + +G+ + +IA   + L+I+  TL      +G+  R LAEA
Sbjct: 206 L-----AAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEA 265

Query: 278 YVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQ 337
           YV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   L+ 
Sbjct: 266 YVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLVLAS 325

Query: 338 KVLKNWGLSSDLIRAEIDAANMQIALGRYDEAI-STLKDVVQHTDKDSETR---AVVFIS 397
             +   G  S++   ++   N+ ++L R+DEA+ S  K +        ET    A VF+ 
Sbjct: 326 MAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVR 385

Query: 398 MGKVLCNQEKFTDAKRCLEIASGIFDKKEANSPV-EAAEAYSEISMQYETMNEFETAISL 457
           + ++     K  ++K   E A  I++K    + V E A   +EIS  YE+++E E A+ L
Sbjct: 386 LAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKL 445

Query: 458 LKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGV 517
           L+KS+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ + G K    
Sbjct: 446 LQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKSAFF 505

Query: 518 GYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMG 559
           G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+  Y AMG
Sbjct: 506 GVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 530

BLAST of Tan0002450 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 5.7e-41
Identity = 155/567 (27.34%), Postives = 257/567 (45.33%), Query Frame = 0

Query: 65  SPSRNLDTLVEKRPQISSRQRKVREKSELEEAFESANTVDEMLKAFKDMESAFEERDLGL 124
           S S NLD  V  R       +K   KS +         V        + E + E  +L  
Sbjct: 89  SNSSNLDLEVMPRDMEKQTGKKNVTKSNVGVGGMRKKKVGGTKLQNGNEEPSSENVELAR 148

Query: 125 ASLKIGLKLDQEGEDPEKALLYAGRALNAFDKD-DNGKPSLLVAMTLQLMGSVNYSLKRF 184
             L     L   G+   KAL    RA   F+   +NGKP L   M L +  +V+  LK +
Sbjct: 149 FLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHVTAAVHCKLKEY 208

Query: 185 SDSLGYLNRANRILGQLEEQGFSAGDVRPVLHAVQLELANVKTAMGRREEAIANLRKCLE 244
           ++++  L R+  I   + E+G      +    A  ++L +    +G+ E +I+   + L 
Sbjct: 209 NEAIPVLQRSVEI--PVVEEGEEHALAK---FAGLMQLGDTYAMVGQLESSISCYTEGLN 268

Query: 245 IKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMKALEIHKKQLGHNSVEVAHDRR 304
           I++  L ++   +G+  R LAEA V  L F +A   C  AL IH++     S+  A DRR
Sbjct: 269 IQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGLPGSIAEAADRR 328

Query: 305 LLGVIYSGLEEHQKALEQNELSQKVLKNWGLSSDLIRAEIDAANMQIALGRYDEAISTLK 364
           L+G+I     +H+ ALE   L+   +   G  S++   +    +  ++L R+DEAI   +
Sbjct: 329 LMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSLSRFDEAICAYQ 388

Query: 365 D--VVQHTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEAN-SPVEA 424
                  T K     AV  V+I +  +     K  +AK   E A  I++      SP E 
Sbjct: 389 KSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYESHNLEISPEEI 448

Query: 425 AEAYSEISMQYETMNEFETAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQE 484
           A   ++IS+  E+MNE E AI+LL+K+L +    P ++     + A++G L  + GK  E
Sbjct: 449 ASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMGVLYYMMGKYME 508

Query: 485 AIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGP 544
           +    + A   L+ +   +    G   N +G A ++L+  + A ++F  AK I++   GP
Sbjct: 509 SYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEEAKCILEQECGP 568

Query: 545 HHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREAQRMLEQLKRK 604
           +H +++    NL+ AY A+G    AI+    VV   E    TA     + +R L QL ++
Sbjct: 569 YHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTEDEKRRLAQLLKE 628

Query: 605 AHGTSKIELSTKALPLPHSPTSSKTLQ 626
           A   +  +  +    +    TSS  L+
Sbjct: 629 AGNVTGRKAKSLKTLIDSDLTSSSALR 650

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O816291.5e-4628.20Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 P... [more]
Q9LII81.3e-4528.97Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 P... [more]
F4HSX98.1e-4027.34Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 P... [more]
A0JM231.1e-1523.38Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2[more]
Q6AZT72.4e-1523.10Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038899312.10.0e+0093.35protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida][more]
XP_023549125.10.0e+0091.80protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo][more]
XP_022954096.10.0e+0091.32protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata][more]
KAG7014584.10.0e+0090.85Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. ar... [more]
KAG6576067.10.0e+0090.85Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
A0A6J1GPX70.0e+0091.32protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JWL00.0e+0090.85protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC11148813... [more]
A0A6J1CRC10.0e+0089.92protein KINESIN LIGHT CHAIN-RELATED 1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A0A0K8P32.6e-30788.17TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 ... [more]
A0A1S3BR153.4e-30788.17nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G31240.19.0e-22065.65Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.11.1e-4728.20Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.19.1e-4728.97Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.27.2e-4428.12Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G27500.15.7e-4127.34Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 578..605
NoneNo IPR availablePFAMPF13424TPR_12coord: 259..324
e-value: 4.3E-8
score: 33.3
coord: 467..531
e-value: 2.7E-9
score: 37.2
NoneNo IPR availablePFAMPF13374TPR_10coord: 218..254
e-value: 6.1E-4
score: 19.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 613..635
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 596..635
NoneNo IPR availablePANTHERPTHR47459KINESIN LIGHT CHAIN-RELATEDcoord: 3..625
NoneNo IPR availablePANTHERPTHR47459:SF1KINESIN LIGHT CHAIN-RELATEDcoord: 3..625
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 377..410
e-value: 35.0
score: 10.2
coord: 299..332
e-value: 450.0
score: 0.4
coord: 460..493
e-value: 240.0
score: 2.9
coord: 167..200
e-value: 210.0
score: 3.4
coord: 544..577
e-value: 30.0
score: 10.8
coord: 419..452
e-value: 5.2
score: 16.0
coord: 215..248
e-value: 9.7
score: 15.1
coord: 339..372
e-value: 72.0
score: 7.4
coord: 502..535
e-value: 31.0
score: 10.6
coord: 257..290
e-value: 18.0
score: 12.7
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 545..572
e-value: 0.0058
score: 16.7
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 419..452
score: 8.6144
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 416..612
e-value: 6.4E-28
score: 99.6
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 90..204
e-value: 2.7E-5
score: 25.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 337..414
e-value: 2.7E-5
score: 26.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 205..333
e-value: 1.0E-14
score: 56.8
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 208..324
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 464..577
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 298..492
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 122..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002450.1Tan0002450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity