Tan0002319 (gene) Snake gourd v1

BLAST of Tan0002319 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 6.2e-109
Identity = 271/780 (34.74%), Postives = 414/780 (53.08%), Query Frame = 0

Query: 13  SVAVAIDKDKFSQQALKWTIERLLSRGQV-LTLLHVKQKPSNSTTNVS-----ANFDSDE 72
           SVAVAI+  K S+  + W +E+ +  G     LL+V+   S   T +      +    D 
Sbjct: 23  SVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDV 82

Query: 73  AAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLG 132
            +AYK +LD  A ++   P++    R  +    ++L+  E +  +   +    +  LV+G
Sbjct: 83  VSAYKQELDWSA-NEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIG 142

Query: 133 APSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQL 192
              R    +  K D+ S+++   P FCTVY I +KGK++S+R S + A            
Sbjct: 143 MSLRGFFSR--KIDMSSLIATAVPRFCTVYVI-SKGKLASVRPSESDASG---------- 202

Query: 193 QPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQD--STISNPPPRVIPDDLEIK- 252
                   +  + ++++  + + P  P        E QD  S +S    RV P    +K 
Sbjct: 203 -------SIRFERSSSTSGSTDSPRLPP-------EYQDFLSAVSEAQSRVSPFSPALKH 262

Query: 253 ---SPFTRPGSRASLGKGQDPFTLDSDISFVSS---GRRSVDRGF-------PPAWNSGS 312
              S        +S G  Q+  +    +  V S   G+++ D  F         A+NS S
Sbjct: 263 SMGSNAVAQMDTSSSGTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMS 322

Query: 313 GGDSYRLSNGSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLES 372
               +R  +    E+ S   ++   ++ M     +      NYS+ +     MS   L  
Sbjct: 323 WTSKWR-DHEDRREMRSSSSSNNHDLVNMDWGAVVP----ENYSWVSHTASHMSDGLL-- 382

Query: 373 DTFSWTSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQR 432
              S  S ++ Q ++  E+ +L+ ELK   +MY+ A  E + A +K  EL++ + EE ++
Sbjct: 383 ---SVHSITDNQVNLNFEIEKLRAELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEK 442

Query: 433 IVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKA 492
           +VE +  EE A   A  EK + + A++ AE  +++   EA  R   E  A+ +A E  K 
Sbjct: 443 LVELKEKEEVAKDTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKL 502

Query: 493 LAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAA 552
            A      + Y+ YT ++I  AT+ F++ LKIG G YG V++  L HT  A+KVL     
Sbjct: 503 QASLVSPGVQYQHYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGET 562

Query: 553 QGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQ 612
           Q   QF QE+E+LS IRHP++VLLLGACPE  CLVYE+M NGSL+D L    D P +PW 
Sbjct: 563 QLSKQFDQELEILSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWF 622

Query: 613 LRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADS 672
            RFRIA E+A+ L+FLH+++P PI+HRDLKPGNILLD N+VSK+ DVGL+ +V      S
Sbjct: 623 ERFRIALEVASALVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSS 682

Query: 673 VTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAI- 732
            T ++ T+  GT  YIDPEYQ+TG++  KSD+YSLGV++LQ+ITA+P I + H V  AI 
Sbjct: 683 RTIFKQTSPVGTLCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIG 742

Query: 733 EKGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEES 770
           +  +F  +LD     WP+ D  + A L + C EMR+RDRPDL   ++P L RLR +A+++
Sbjct: 743 DDAEFMAILDKKAGSWPISDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKA 764

BLAST of Tan0002319 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 379.4 bits (973), Expect = 1.0e-103
Identity = 256/770 (33.25%), Postives = 408/770 (52.99%), Query Frame = 0

Query: 13  SVAVAIDKDKFSQQALKWTIERLLSRGQV-LTLLHVKQKPSNSTTNVS-----ANFDSDE 72
           +V VA+     S+  + W IE+  + G V   LLH+    ++  T +      +    D 
Sbjct: 21  TVVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDV 80

Query: 73  AAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLG 132
             AY+ ++     ++   P+     R  +    +++E   ++  +   V ++ ID +V+G
Sbjct: 81  VTAYRQEI-LWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIG 140

Query: 133 APSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQL 192
             SR+   +  K+DI S++S   P+FCTVY + +KGK+S +R S +   A  I    S  
Sbjct: 141 GSSRSFFSR--KADICSVISALMPNFCTVYVV-SKGKLSCVRPSDSDGNA-TIREDGS-- 200

Query: 193 QPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDLEIKSPF 252
                      + TN+S  ++         +S   ++Q   +S P  R +     I    
Sbjct: 201 -----------ERTNSSSGSSGPTSDSSDVMSSAHDSQSRPLSLPVRR-MQHFPAIAGQA 260

Query: 253 TRPGSRASLGKGQDP-FTLDS----DISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNGS 312
           + P   +S+G  +    +LD+    D+S ++         + P             S+ S
Sbjct: 261 SVPMETSSVGSDETRCMSLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSS 320

Query: 313 EFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSNG 372
             E  + G  +     GMG                 T+ +     S  SD  S  S+++ 
Sbjct: 321 NREYGNFG--TRFSWSGMG--------------VDTTHSRASQQASNMSDALSEQSYTDN 380

Query: 373 QDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAEEAA 432
           Q ++  E+ +L+ EL+   +MY+ A  E  +A +K  EL++ +LEE  ++ E ++ E  A
Sbjct: 381 QVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEA 440

Query: 433 LAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGDIGY 492
             +AE EK   + A   AE+ ++ A  E  +R   E+ +  + +E +K     G   + Y
Sbjct: 441 RELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQY 500

Query: 493 RRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQEVE 552
           + +  ++I  AT+ FS+ LKIG G YG V++  L HT   +KVL+    Q   QFQQE+E
Sbjct: 501 QHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELE 560

Query: 553 VLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAEIAT 612
           +LS IRHP++VLLLGACPE   LVYE+M NGSLED LF+  + P LPW  RFRIA E+A 
Sbjct: 561 ILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAA 620

Query: 613 GLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVP-PSMADSVTQYRMTATA 672
            L+FLH+++P+PI+HRDLKP NILLD N+VSK+ DVGL+ +V    ++   T Y+ T+  
Sbjct: 621 ALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPV 680

Query: 673 GTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKG-KFAEMLD 732
           GT  YIDPEYQ+TG +  KSDIYS G++LLQ++TA+P I L H V  A++   +F ++LD
Sbjct: 681 GTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILD 740

Query: 733 PDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEES 770
               +WP+E+  + A L++ C E+R +DRPDL   +LP L  L+ +AE++
Sbjct: 741 QKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKA 755

BLAST of Tan0002319 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.4e-102
Identity = 201/430 (46.74%), Postives = 274/430 (63.72%), Query Frame = 0

Query: 356 TSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEAR 415
           TS  + + ++E E+ RLK EL+ T+  Y  AC+E  + + K K L    L E +R+  A 
Sbjct: 302 TSRKSKKVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRVNNAV 361

Query: 416 MAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFG 475
             EE     A  EK +   A++  E A+ + A E  +R   E  A     E KK +    
Sbjct: 362 EKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLL 421

Query: 476 HGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQ 535
             D  YR+YTI++I TAT GFS    IGEGGYG V++  LD T  A+KV+R D  + + +
Sbjct: 422 GTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQE 481

Query: 536 FQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRI 595
           F +EVEVLS +RHP++VLLLGACPEN CLVYE++ NGSLE+ +F + + P LPW +RFR+
Sbjct: 482 FLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRV 541

Query: 596 AAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYR 655
             E+A GL FLH ++PEPIVHRDLKPGNILL+RNYVSKI+DVGLA+LV     D+VT YR
Sbjct: 542 IFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYR 601

Query: 656 MTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFA 715
            +  AGT  YIDPEY +TG +  KSD+Y+ G+++LQ++TAR P G+  AV  A++KG   
Sbjct: 602 NSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKKGTLT 661

Query: 716 EMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPY 775
           EMLD  V DWP+ +  + A++ ++CAE R RDRPDL   V+P L RL   A   +     
Sbjct: 662 EMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVLKRLVETANSKVKKEGS 721

Query: 776 SISSSSSSLC 786
           ++ + S   C
Sbjct: 722 NLRAPSHYFC 731

BLAST of Tan0002319 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 372.9 bits (956), Expect = 9.7e-102
Identity = 272/769 (35.37%), Postives = 414/769 (53.84%), Query Frame = 0

Query: 14  VAVAI-DKDKFSQQALKWTIERLLSRGQVL-TLLHVKQKPSNSTTNVSANFDSDEAAAYK 73
           VAVAI   +  ++  ++W ++   S+  V+  LLHV+ + SNS +    +  +   + YK
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTT---SVYK 67

Query: 74  HQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLGAPSRT 133
             +D   R +  LP R       +  + ++LE  +I+  +   V  + I  LV+GA S  
Sbjct: 68  KDVDRKTR-EMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASS-- 127

Query: 134 SIV---KRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQLQP 193
           SI+   K  +S++ S ++   P FC+V+ I +KGK+ ++R+S        IA  RS+ + 
Sbjct: 128 SIIFSWKLKRSNLSSRIADATPRFCSVHVI-SKGKLLNVRKSDMDTETS-IADDRSESRF 187

Query: 194 APPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDLEIKSPFTR 253
           +       H  T +S   + H +     L + ++   +        +   + E   P   
Sbjct: 188 SSDS----HSGTVSS--TSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNE---PHHH 247

Query: 254 PGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNGSEFEIFSP 313
             +RA         +LD D S + +      +GF            YR S+         
Sbjct: 248 HHNRAG--------SLDVDESKLLN-----QKGF------------YRTSSSG------- 307

Query: 314 GPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSNGQDDVEAE 373
                   +G GG        ++  S+ ++  +E SS    S T+S  + S+ Q   + E
Sbjct: 308 --------IGYGG--------SDISSWRSSQMEEASS----SSTYSDPTSSSSQIHKDFE 367

Query: 374 MRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAEEAALAIAENE 433
           + +LK+EL+    MY+ A  E ++A +K ++L++ + EE  R+    + EE A  + E E
Sbjct: 368 LEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEADEVVEME 427

Query: 434 KLKCKAALEAAEAAQKIAALEARKRASVEKMAD---MEAEETKKALAGFGHGDIGYRRYT 493
           + + + A   AE  ++    E  +R   E  A+    E +  + AL G       Y ++ 
Sbjct: 428 RERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPLQRQQYMKFE 487

Query: 494 IQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSC 553
            ++I  AT+ FSD LKIG GGYG V+R  L HT VA+KVL  D +    QF QE+E+LS 
Sbjct: 488 WEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEILSK 547

Query: 554 IRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRK------PDDPVLPWQLRFRIAAEI 613
           IRHP+++LLLGACPE   LVYE+M NGSLE+ L ++      P  P L W  RFRIA EI
Sbjct: 548 IRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIAWEI 607

Query: 614 ATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTAT 673
           A+ L FLH   P PIVHRDLKP NILLDRN VSKI DVGL+++V    + + T +  T  
Sbjct: 608 ASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNETGP 667

Query: 674 AGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAI--EKGKFAEM 733
            GTFFYIDPEYQ+TG++  +SDIY+ G++LLQ++TAR  +GLAH++ +A+  + GKF E+
Sbjct: 668 VGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQTGKFTEI 707

Query: 734 LDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMA 767
           LD    DWPV++A +   + ++CAEMRKRDRPDLGK +LP L RL+ +A
Sbjct: 728 LDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVA 707

BLAST of Tan0002319 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 5.1e-87
Identity = 238/789 (30.16%), Postives = 391/789 (49.56%), Query Frame = 0

Query: 7   AQKKEESVAVAIDKDKFSQQALKWTIERLLS-RGQVLTLLHVKQK----PSNSTTNVSAN 66
           A  +  +VA+AI     S+  +KW + +  S +     L+H+  K    P+ S   VS +
Sbjct: 27  APSEPMTVALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSIS 86

Query: 67  FDSDE-AAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEV--------------MLEDGE 126
            + +E AAAY+ ++    ++    PF+  C R  +  +E               +LE   
Sbjct: 87  EELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNS 146

Query: 127 ISKTLINYVNKNVIDILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSS 186
           ++  +   VN+++I  L++G  S+ +  + +  DI + +S    + CTVY + N G V  
Sbjct: 147 VAVAITKEVNQHLISNLIIGRSSQAASSRNY--DITASISASVSNLCTVYVVSN-GGVHI 206

Query: 187 LRQSTAPAPAKPIAPSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDS 246
           L + T+       +              +       S   +         +S  L++   
Sbjct: 207 LAKDTSDTERNDTS--------------IESGFERTSSSCSSGSGANSDVMSNALKSNPH 266

Query: 247 TISNPPPRVIPDDLE-IKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPA 306
           T+SN   + +P  +  +  P     + +   K +      SD +  +S R S +     +
Sbjct: 267 TLSNKRMQNLPTIVRGVSVPMETSSTESDETKKR-----SSDAAEEASKRSSPETSRSVS 326

Query: 307 WNSGSGGDSYRLSNGSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSS 366
           WN              +F  F     + S M             ++N+ + N        
Sbjct: 327 WN-------------PQFRDFDERKDAMSSM-------------SSNFEYGNVVTPLGHY 386

Query: 367 CSLESDTFSWTSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKL 426
            +   DT +             E+ +L+ EL+   +MY+ A  E L+A +K  EL     
Sbjct: 387 FTDNQDTLN-------------EISKLRAELRHAHEMYAVAQVETLDASRKLNELK---- 446

Query: 427 EEEQRIVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAE 486
            EE  ++E    E   +A  E EK + K   E  EAAQ+    EA  +A+ E     + E
Sbjct: 447 FEELTLLE---HETKGIAKKETEKFEQKRR-EEREAAQR---REAEMKATHEAKEKEKLE 506

Query: 487 ETKKALAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVL 546
           E+           + Y+ +T ++I  AT+ FS+ LKIG G YG V++  L HT  A+KVL
Sbjct: 507 ESSLVAP-----KLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVL 566

Query: 547 RPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDP 606
               +    QF QE+E+LS IRHP++VLLLGACP++  LVYE+M NGSLED LF+  D  
Sbjct: 567 HSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQ 626

Query: 607 VLPWQLRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPP 666
            +PW +R RIA E+A+ L+FLH+++P PI+HRDLKP NILL+ N+VSK+ DVGL+ ++  
Sbjct: 627 PIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQA 686

Query: 667 S--MADSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAH 726
           +  ++   T Y+ T+  GT  YIDPEYQ+TG +  KSD+Y+ G+++LQ++T +  + L +
Sbjct: 687 ADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTY 738

Query: 727 AVGRAIEKG---KFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELN 770
            V  A+E     +  ++LD    +WP+E+  + A L++QC E+R +DRPDL   +LP L 
Sbjct: 747 TVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLE 738

BLAST of Tan0002319 vs. NCBI nr
Match: XP_022937745.1 (U-box domain-containing protein 52-like [Cucurbita moschata])

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 682/857 (79.58%), Postives = 734/857 (85.65%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV +
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGS 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
             DSD+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINYVNKNVI
Sbjct: 61  YSDSDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT +VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S +Q QPA  RPQLLHQLTNASEV+ EHP   R+Q S++LETQDS  SNPP R I +D 
Sbjct: 181 TSHNQPQPA-TRPQLLHQLTNASEVSVEHPTILRSQPSRHLETQDSLASNPPFRAIHEDF 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNG 300
           EIKSPF RPGSRAS+GKGQD  T DSDISFVSSGR+S+DRG  PAWNSG+ GDSYRLSNG
Sbjct: 241 EIKSPFNRPGSRASMGKGQDTSTADSDISFVSSGRQSLDRGNSPAWNSGT-GDSYRLSNG 300

Query: 301 SEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSN 360
           SE    SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMSS S ES+T SW S S+
Sbjct: 301 SETS--SPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSSAS-ESET-SWASQSH 360

Query: 361 GQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQK------------------------ 420
            Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQK                        
Sbjct: 361 AQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKISNLFSMIYNHDSTSNSN 420

Query: 421 -----AKELHRWKLEEEQRIVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEAR 480
                A ELHRWK+EEE R+ EARMAEEAALAIAE EKLKCKAAL+AAE AQ+ A  EAR
Sbjct: 421 RLIWQASELHRWKMEEEHRLEEARMAEEAALAIAEQEKLKCKAALQAAELAQRSAVQEAR 480

Query: 481 KRASVEKMADMEAEETKKALAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVF 540
           KRA+VEK+A MEAEETKKAL GFGHGDIGYRRY+IQ+IETATNGFS ALKIGEGGYGPV+
Sbjct: 481 KRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVY 540

Query: 541 RGELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMAN 600
           RGELDHTQVAIKVLRPDAAQGRSQFQ+EVEVLSCIRHPNMVLLLGACPEN CLVYEFMAN
Sbjct: 541 RGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMAN 600

Query: 601 GSLEDCLFRKPDDPVLPWQLRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYV 660
           GSLEDCLFRKP+DPVLPWQLRFRIA EIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYV
Sbjct: 601 GSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYV 660

Query: 661 SKISDVGLARLVPPSMADSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQ 720
           SKISDVGLARLVPPS+A+SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQ
Sbjct: 661 SKISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQ 720

Query: 721 IITARPPIGLAHAVGRAIEKGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDL 780
           IITA+PPIGLAH V RAIEKGKFAEMLDPDVKDWPVE+ALKFAKLSIQCAEMRKRDRPDL
Sbjct: 721 IITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDL 780

Query: 781 GKVVLPELNRLRAMAEESLGIRPYSISSSSSSLCASQASSSQELISLHQG--HYGSWVES 827
           GKVVLPEL+RLRAMAEES+G +P+SI  S    C SQAS SQE  SLHQG     S    
Sbjct: 781 GKVVLPELSRLRAMAEESMGFQPFSICDSP---CMSQASFSQEQFSLHQGLDECESSYCQ 840

BLAST of Tan0002319 vs. NCBI nr
Match: XP_023537969.1 (U-box domain-containing protein 52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 679/856 (79.32%), Postives = 730/856 (85.28%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV  
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGT 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
             DSD+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINYVNKNVI
Sbjct: 61  YSDSDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S +Q  PA  RPQLLHQLTNASEV+ EHP   R+Q S++LETQDS  SNPP R I +D 
Sbjct: 181 TSHNQPHPA-TRPQLLHQLTNASEVSVEHPIILRSQPSRHLETQDSVASNPPSRTIHEDF 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNG 300
           EIKSPF RPGSRAS+GKGQD    DSDISFVSSGR+S+DRG PPAWNSG+  DSYRLSNG
Sbjct: 241 EIKSPFNRPGSRASMGKGQDTSMADSDISFVSSGRQSLDRGNPPAWNSGT-VDSYRLSNG 300

Query: 301 SEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSN 360
           SE    SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMSS S ES+T SW S S+
Sbjct: 301 SETS--SPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSSAS-ESET-SWASQSH 360

Query: 361 GQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQK------------------------ 420
            Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQK                        
Sbjct: 361 AQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKITNLFSMIYNHDSTIEFQ 420

Query: 421 ----AKELHRWKLEEEQRIVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARK 480
               A ELHRWK+EEE R+ EA+MAE AALAIAE EK+KCKAAL+AAE AQ+ A  EARK
Sbjct: 421 LIWQASELHRWKMEEEHRLEEAKMAEGAALAIAEKEKMKCKAALQAAEVAQRSAVHEARK 480

Query: 481 RASVEKMADMEAEETKKALAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFR 540
           RA+VEK+A MEAEETKKAL GFGHGDIGYRRY+IQ+IETATNGFS ALKIGEGGYGPV+R
Sbjct: 481 RANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVYR 540

Query: 541 GELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANG 600
           GELDHTQVAIKVLRPDAAQGRSQFQ+EVEVLSCIRHPNMVLLLGACPEN CLVYEFMANG
Sbjct: 541 GELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANG 600

Query: 601 SLEDCLFRKPDDPVLPWQLRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVS 660
           SLEDCLFRKP+DPVLPWQLRFRIA EIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVS
Sbjct: 601 SLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVS 660

Query: 661 KISDVGLARLVPPSMADSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQI 720
           KISDVGLARLVPPS+A+SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQI
Sbjct: 661 KISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQI 720

Query: 721 ITARPPIGLAHAVGRAIEKGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLG 780
           ITA+PPIGLAH V RAIEKGKFAEMLDPDVKDWPVE+ALKFAKLSIQCAEMRKRDRPDLG
Sbjct: 721 ITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLG 780

Query: 781 KVVLPELNRLRAMAEESLGIRPYSISSSSSSLCASQASSSQELISLHQG--HYGSWVESS 827
           KVVLPEL+RLRAMAEES+G +PYSI  S    C SQAS SQE  SLH G     S    S
Sbjct: 781 KVVLPELSRLRAMAEESMGFQPYSICDSP---CMSQASFSQERFSLHHGLDESESSYCQS 840

BLAST of Tan0002319 vs. NCBI nr
Match: XP_022966072.1 (U-box domain-containing protein 52-like [Cucurbita maxima])

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 660/795 (83.02%), Postives = 708/795 (89.06%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV A
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGA 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
             D D+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINY NKN I
Sbjct: 61  CSDLDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S SQ QPA  RPQLLHQLTNAS+ + EHP   R+Q SK+LETQDS  SNPP R I +D 
Sbjct: 181 TSHSQPQPA-SRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDF 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSN- 300
           EIKSPF RPGSRA +GKGQD  T DSDISF+SSGR+S+DRG  PAWNS S  DSYRLSN 
Sbjct: 241 EIKSPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNS-STRDSYRLSNG 300

Query: 301 -GSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSH 360
            GSE E  SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMSS S ES+T SW S 
Sbjct: 301 SGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSSAS-ESET-SWASQ 360

Query: 361 SNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAE 420
           S+ Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQKA ELHRWK+EEE R+ EARMAE
Sbjct: 361 SHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAE 420

Query: 421 EAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGD 480
            AALAIAE EKLKCKAAL+AAE AQ+ A  EARKRA+VEK+A MEAEETKKAL GFGHGD
Sbjct: 421 GAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGD 480

Query: 481 IGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQ 540
           IGYRRY+IQ+IETATNGFS +LKIGEGGYGPV+RGELDHTQVAIKVLRPDAAQGRSQFQ+
Sbjct: 481 IGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQR 540

Query: 541 EVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAE 600
           EVEVLSCIRHPNMVLLLGACPEN CLVYEFMANGSLEDCLFRKP+DPVLPWQLRFRIA E
Sbjct: 541 EVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEE 600

Query: 601 IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTA 660
           IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPS+A+SVTQYRMTA
Sbjct: 601 IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTA 660

Query: 661 TAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFAEML 720
           TAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQIITA+PPIGLAH V RAIEKGKFAEML
Sbjct: 661 TAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEML 720

Query: 721 DPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPYSIS 780
           DPDVKDWPVE+ALKFAKLSIQCAEMRKRDRPDLGKVVLPEL+RLRAMAEES+GI+PYSI 
Sbjct: 721 DPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSIC 780

Query: 781 SSSSSLCASQASSSQ 794
            S    C SQAS SQ
Sbjct: 781 DSP---CMSQASFSQ 784

BLAST of Tan0002319 vs. NCBI nr
Match: KAG6586127.1 (U-box domain-containing protein 52, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 665/830 (80.12%), Postives = 714/830 (86.02%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV +
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGS 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
             DSD+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINYVNKNVI
Sbjct: 61  YSDSDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT +VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S +Q QPA  RPQLLHQLTNASEV+ EHP   R+Q S++LETQDS  SNPP R I +D 
Sbjct: 181 TSHNQPQPA-TRPQLLHQLTNASEVSVEHPIILRSQPSRHLETQDSVASNPPSRAIHEDF 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSN- 300
           EIKSPF RPGSRAS GKGQD  T DSDISFVSSGR+S+DRG  PAWNSG+ GD YRLSN 
Sbjct: 241 EIKSPFNRPGSRASTGKGQDTSTADSDISFVSSGRQSLDRGNSPAWNSGT-GDLYRLSNG 300

Query: 301 -GSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSH 360
            GSEFE  SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMS  S ES+T SW S 
Sbjct: 301 SGSEFETSSPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSFAS-ESET-SWASQ 360

Query: 361 SNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQK---------------------- 420
           S+ Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQK                      
Sbjct: 361 SHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKISNLFSMIYNHDSTSN 420

Query: 421 -------AKELHRWKLEEEQRIVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALE 480
                  A ELHRWK+EEE R+ EARMAEEAALAIAE EKLKCKAAL+AAE AQ+ A  E
Sbjct: 421 SNRLIWQASELHRWKMEEEHRLEEARMAEEAALAIAEQEKLKCKAALQAAEVAQRSAVQE 480

Query: 481 ARKRASVEKMADMEAEETKKALAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGP 540
           ARKRA+VEK+A MEAEETKKAL GFGHGDIGYRRY+IQ+IETATNGFS ALKIGEGGYGP
Sbjct: 481 ARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGP 540

Query: 541 VFRGELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFM 600
           V+RGELDHTQVAIKVLRPDAAQGRSQFQ+EVEVLSCIRHPNMVLLLGACPEN CLVYEFM
Sbjct: 541 VYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFM 600

Query: 601 ANGSLEDCLFRKPDDPVLPWQLRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRN 660
           ANGSLEDCLFRKP+DPVLPWQLRFRIA EIATGLLFLHQTRPEPIVHRDLKPGNILLDRN
Sbjct: 601 ANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRN 660

Query: 661 YVSKISDVGLARLVPPSMADSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVML 720
           YVSKISDVGLARLVPPS+A+SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSD+YSLGVML
Sbjct: 661 YVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVML 720

Query: 721 LQIITARPPIGLAHAVGRAIEKGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRP 780
           LQIITA+PPIGLAH V RAIEKGKFAEMLDPDVKDWPVE+ALKFAKLSIQCAEMRKRDRP
Sbjct: 721 LQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRP 780

Query: 781 DLGKVVLPELNRLRAMAEESLGIRPYSISSSSSSLCASQASSSQELISLH 800
           DLGKVVLPEL+RLRAMAEES+G +PYSI  S    C SQAS SQE    H
Sbjct: 781 DLGKVVLPELSRLRAMAEESMGFQPYSICDSP---CMSQASFSQEAARHH 819

BLAST of Tan0002319 vs. NCBI nr
Match: KAG7020948.1 (U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 635/795 (79.87%), Postives = 683/795 (85.91%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV +
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGS 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
           N DSD+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINYVNKNVI
Sbjct: 61  NSDSDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT +VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S +Q QPA  RPQLLHQLTNASEV+ EHP   R+Q S                      
Sbjct: 181 TSHNQPQPA-TRPQLLHQLTNASEVSVEHPIILRSQPS---------------------- 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSN- 300
                           + QD  T DSDISFVSSGR+S+DRG  PAWNSG+ GD YRLSN 
Sbjct: 241 ----------------RRQDTSTADSDISFVSSGRQSLDRGNSPAWNSGT-GDLYRLSNG 300

Query: 301 -GSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSH 360
            GSEFE  SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMSS S ES+T SW S 
Sbjct: 301 SGSEFETSSPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSSAS-ESET-SWASQ 360

Query: 361 SNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAE 420
           S+ Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQKA ELHRWK+EEE R+ EARMAE
Sbjct: 361 SHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKIEEEHRLEEARMAE 420

Query: 421 EAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGD 480
           EAALAIAE EKLKCKAAL+AAE AQ+ A  EARKRA+VEK+A MEAEETKKAL GFGHGD
Sbjct: 421 EAALAIAEQEKLKCKAALQAAEVAQRSAVQEARKRANVEKLAVMEAEETKKALEGFGHGD 480

Query: 481 IGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQ 540
           IGYRRY+IQ+IETATNGFS ALKIGEGGYGPV+RGELDHTQVAIKVLRPDAAQGRSQFQ+
Sbjct: 481 IGYRRYSIQEIETATNGFSAALKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQR 540

Query: 541 EVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAE 600
           EVEVLSCIRHPNMVLLLGACPEN CLVYEFMANGSLEDCLFRKP++PVLPWQLRFRIA E
Sbjct: 541 EVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPENPVLPWQLRFRIAEE 600

Query: 601 IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTA 660
           IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPS+A+SVTQYRMTA
Sbjct: 601 IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTA 660

Query: 661 TAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFAEML 720
           TAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQIITA+PPIGLAH V RAIEKGKFAEML
Sbjct: 661 TAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEML 720

Query: 721 DPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPYSIS 780
           DPDVKDWPVE+ALKFAKLSIQCAEMRKRDRPDLGKVVLPEL+RLRAMAEES+G +PYSI 
Sbjct: 721 DPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGFQPYSIC 746

Query: 781 SSSSSLCASQASSSQ 794
            S    C SQAS SQ
Sbjct: 781 DSP---CMSQASFSQ 746

BLAST of Tan0002319 vs. ExPASy TrEMBL
Match: A0A6J1FHP0 (U-box domain-containing protein 52-like OS=Cucurbita moschata OX=3662 GN=LOC111444053 PE=4 SV=1)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 682/857 (79.58%), Postives = 734/857 (85.65%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV +
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGS 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
             DSD+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINYVNKNVI
Sbjct: 61  YSDSDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT +VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S +Q QPA  RPQLLHQLTNASEV+ EHP   R+Q S++LETQDS  SNPP R I +D 
Sbjct: 181 TSHNQPQPA-TRPQLLHQLTNASEVSVEHPTILRSQPSRHLETQDSLASNPPFRAIHEDF 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNG 300
           EIKSPF RPGSRAS+GKGQD  T DSDISFVSSGR+S+DRG  PAWNSG+ GDSYRLSNG
Sbjct: 241 EIKSPFNRPGSRASMGKGQDTSTADSDISFVSSGRQSLDRGNSPAWNSGT-GDSYRLSNG 300

Query: 301 SEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSN 360
           SE    SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMSS S ES+T SW S S+
Sbjct: 301 SETS--SPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSSAS-ESET-SWASQSH 360

Query: 361 GQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQK------------------------ 420
            Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQK                        
Sbjct: 361 AQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKISNLFSMIYNHDSTSNSN 420

Query: 421 -----AKELHRWKLEEEQRIVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEAR 480
                A ELHRWK+EEE R+ EARMAEEAALAIAE EKLKCKAAL+AAE AQ+ A  EAR
Sbjct: 421 RLIWQASELHRWKMEEEHRLEEARMAEEAALAIAEQEKLKCKAALQAAELAQRSAVQEAR 480

Query: 481 KRASVEKMADMEAEETKKALAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVF 540
           KRA+VEK+A MEAEETKKAL GFGHGDIGYRRY+IQ+IETATNGFS ALKIGEGGYGPV+
Sbjct: 481 KRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVY 540

Query: 541 RGELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMAN 600
           RGELDHTQVAIKVLRPDAAQGRSQFQ+EVEVLSCIRHPNMVLLLGACPEN CLVYEFMAN
Sbjct: 541 RGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMAN 600

Query: 601 GSLEDCLFRKPDDPVLPWQLRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYV 660
           GSLEDCLFRKP+DPVLPWQLRFRIA EIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYV
Sbjct: 601 GSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYV 660

Query: 661 SKISDVGLARLVPPSMADSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQ 720
           SKISDVGLARLVPPS+A+SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQ
Sbjct: 661 SKISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQ 720

Query: 721 IITARPPIGLAHAVGRAIEKGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDL 780
           IITA+PPIGLAH V RAIEKGKFAEMLDPDVKDWPVE+ALKFAKLSIQCAEMRKRDRPDL
Sbjct: 721 IITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDL 780

Query: 781 GKVVLPELNRLRAMAEESLGIRPYSISSSSSSLCASQASSSQELISLHQG--HYGSWVES 827
           GKVVLPEL+RLRAMAEES+G +P+SI  S    C SQAS SQE  SLHQG     S    
Sbjct: 781 GKVVLPELSRLRAMAEESMGFQPFSICDSP---CMSQASFSQEQFSLHQGLDECESSYCQ 840

BLAST of Tan0002319 vs. ExPASy TrEMBL
Match: A0A6J1HM11 (U-box domain-containing protein 52-like OS=Cucurbita maxima OX=3661 GN=LOC111465786 PE=4 SV=1)

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 660/795 (83.02%), Postives = 708/795 (89.06%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSA 60
           MAR GGA KK+ESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVK KPS+STTNV A
Sbjct: 1   MAR-GGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGA 60

Query: 61  NFDSDEAAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVI 120
             D D+A A+K Q+DN A  QFFLPFRC CTRSNIPCNEV LE+G+ISK LINY NKN I
Sbjct: 61  CSDLDDAVAHKQQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAI 120

Query: 121 DILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIA 180
           D+LVLGAPSRT IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSS+RQ+T+PAPAKPI+
Sbjct: 121 DVLVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPIS 180

Query: 181 PSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDL 240
            S SQ QPA  RPQLLHQLTNAS+ + EHP   R+Q SK+LETQDS  SNPP R I +D 
Sbjct: 181 TSHSQPQPA-SRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDF 240

Query: 241 EIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSN- 300
           EIKSPF RPGSRA +GKGQD  T DSDISF+SSGR+S+DRG  PAWNS S  DSYRLSN 
Sbjct: 241 EIKSPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNS-STRDSYRLSNG 300

Query: 301 -GSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSH 360
            GSE E  SPG ASPSPMLGMGG +SID+NNN++Y  SN Y  EMSS S ES+T SW S 
Sbjct: 301 SGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSY--SNPYSHEMSSAS-ESET-SWASQ 360

Query: 361 SNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAE 420
           S+ Q+DVEAEMRRLKLELKQTMDMYSTACKEALNAKQKA ELHRWK+EEE R+ EARMAE
Sbjct: 361 SHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAE 420

Query: 421 EAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGD 480
            AALAIAE EKLKCKAAL+AAE AQ+ A  EARKRA+VEK+A MEAEETKKAL GFGHGD
Sbjct: 421 GAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGD 480

Query: 481 IGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQ 540
           IGYRRY+IQ+IETATNGFS +LKIGEGGYGPV+RGELDHTQVAIKVLRPDAAQGRSQFQ+
Sbjct: 481 IGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQR 540

Query: 541 EVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAE 600
           EVEVLSCIRHPNMVLLLGACPEN CLVYEFMANGSLEDCLFRKP+DPVLPWQLRFRIA E
Sbjct: 541 EVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEE 600

Query: 601 IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTA 660
           IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPS+A+SVTQYRMTA
Sbjct: 601 IATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTA 660

Query: 661 TAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFAEML 720
           TAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQIITA+PPIGLAH V RAIEKGKFAEML
Sbjct: 661 TAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEML 720

Query: 721 DPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPYSIS 780
           DPDVKDWPVE+ALKFAKLSIQCAEMRKRDRPDLGKVVLPEL+RLRAMAEES+GI+PYSI 
Sbjct: 721 DPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSIC 780

Query: 781 SSSSSLCASQASSSQ 794
            S    C SQAS SQ
Sbjct: 781 DSP---CMSQASFSQ 784

BLAST of Tan0002319 vs. ExPASy TrEMBL
Match: A0A6J1EUU5 (U-box domain-containing protein 35-like OS=Cucurbita moschata OX=3662 GN=LOC111436140 PE=4 SV=1)

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 618/826 (74.82%), Postives = 688/826 (83.29%), Query Frame = 0

Query: 1   MARGGGAQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPS---NSTTN 60
           MAR GGAQKKE  + VAIDKDK SQQALKWTI+RLLSRGQV+TLLHVK K S   N+TTN
Sbjct: 1   MAR-GGAQKKE--MVVAIDKDKGSQQALKWTIDRLLSRGQVVTLLHVKHKSSSTANATTN 60

Query: 61  VSANFDSDEAAAYK-HQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVN 120
           + A+FD  E A YK H +DNH  DQFFLPFRC CT SNI CNEVMLE  +I KTLINYV+
Sbjct: 61  LKADFDHGETALYKHHHIDNHINDQFFLPFRCICTSSNITCNEVMLEGSDIPKTLINYVS 120

Query: 121 KNVIDILVLGAPSRTS--IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPA 180
           KNV+DILV+GAP+R++  I KRFKSD+PS VSKGAPDFCTVYTIG++GKV S+RQ T P 
Sbjct: 121 KNVVDILVMGAPTRSAAQIYKRFKSDVPSSVSKGAPDFCTVYTIGHRGKV-SVRQPTVPV 180

Query: 181 PAKPIAPSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPR 240
           PA+PIAP R+Q   A PRPQLLHQ TN S++ AE P    ++ S+ LETQ S      P 
Sbjct: 181 PARPIAPPRNQSLRASPRPQLLHQRTNVSQLLAE-PNPLWSKSSRCLETQSSM---RVPH 240

Query: 241 VIPDDLEIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDS 300
            +   L+ KSPFTR GSRAS+ K  DP T+DSD SFVS GRRSVDRGF  A +  +  DS
Sbjct: 241 ALQQYLDFKSPFTRQGSRASVSKRVDPSTVDSDNSFVSCGRRSVDRGFSGALSYATCRDS 300

Query: 301 YRLSNGSEFEIFSPGPASPSPM----LGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLES 360
           YRLSN SE E  SPGP  PSP     +  G  KSI NNN ++YS      Q++SSCSLES
Sbjct: 301 YRLSNVSESETSSPGPPPPSPSPTLEIESGMNKSIHNNNFSSYS------QDLSSCSLES 360

Query: 361 DTFSWTSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQR 420
           DTF+  SHS+ QDDVEAEMRRLKLELKQTMDMYSTACKEALNA +KA ELHRWK+EEEQR
Sbjct: 361 DTFTRPSHSHAQDDVEAEMRRLKLELKQTMDMYSTACKEALNANRKATELHRWKVEEEQR 420

Query: 421 IVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKA 480
             EARMAEEAALA+AE EK+KC+AA++AAEAAQKIA LEARKR + E+MADMEAEETKKA
Sbjct: 421 FEEARMAEEAALAMAEREKMKCQAAIKAAEAAQKIAELEARKRENAERMADMEAEETKKA 480

Query: 481 LAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAA 540
           L+GFGHGDIGYR+YTI++IE ATN FSD+LKIGEGGYGPVFRG+LDHT VAIKVLRPDAA
Sbjct: 481 LSGFGHGDIGYRKYTIEEIEIATNHFSDSLKIGEGGYGPVFRGKLDHTPVAIKVLRPDAA 540

Query: 541 QGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQ 600
            GRSQFQQEVEVLSCIRHPNMVLLLGACPEN CLVYEFM NGSLEDCLFRK ++PVL WQ
Sbjct: 541 HGRSQFQQEVEVLSCIRHPNMVLLLGACPENGCLVYEFMGNGSLEDCLFRKANNPVLSWQ 600

Query: 601 LRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADS 660
           LRFRIAAEIATGLLFLHQ RPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVP S+ADS
Sbjct: 601 LRFRIAAEIATGLLFLHQHRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPLSVADS 660

Query: 661 VTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIE 720
           VTQYRMTATAGTF YIDPEYQ TGLLGI+SDIYSLGVMLLQI+T RPPIGLAHAV RAI+
Sbjct: 661 VTQYRMTATAGTFCYIDPEYQLTGLLGIQSDIYSLGVMLLQIVTGRPPIGLAHAVERAIQ 720

Query: 721 KGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESL 780
           KGKFAEMLDP+VKDWP EDALKFAKLS++C EMRKRDRPDLGKVVLPELNRLRAMAEES+
Sbjct: 721 KGKFAEMLDPEVKDWPFEDALKFAKLSMKCTEMRKRDRPDLGKVVLPELNRLRAMAEESM 780

Query: 781 GIRPYSISSSSSSLCASQASSSQELISLHQGHYGSWVESSGSSMSS 817
           GIRP++ SS+SSS   SQ SSSQE + +HQ   G+WVESS S  +S
Sbjct: 781 GIRPFNGSSASSSPYTSQTSSSQEPMRIHQEQCGAWVESSSSQSNS 812

BLAST of Tan0002319 vs. ExPASy TrEMBL
Match: A0A5A7TVP5 (U-box domain-containing protein 34-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G00530 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 4.7e-277
Identity = 523/719 (72.74%), Postives = 589/719 (81.92%), Query Frame = 0

Query: 144 IVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQLQPAPPRPQLLHQLTNAS 203
           +VSKGAPDFCTVY I NKGKV +++QSTAPAPAKP+A  R+Q+QP PPRPQLLHQLTNAS
Sbjct: 1   MVSKGAPDFCTVYIIANKGKV-TVKQSTAPAPAKPVASPRTQIQPPPPRPQLLHQLTNAS 60

Query: 204 EVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDLEIK-------------------- 263
           EV++    +  +Q    LETQ S    P P    +D +IK                    
Sbjct: 61  EVSSSETNKSCSQSG--LETQISIKKVPLPS-FQEDWDIKYVSSLALSHIHMCLYCYNRW 120

Query: 264 ------SPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNS--GSGGDSY 323
                 SPFTRPGSRAS+ K  DP  +DSDISFVSSGRRS D     AWNS   +GGDS+
Sbjct: 121 RYIVGRSPFTRPGSRASMSKVMDPLMVDSDISFVSSGRRSTDYCLSGAWNSNIATGGDSF 180

Query: 324 RLSNGSEFEIFSPGPASPSPM-LGMGGTKSIDNNNNNNYS-FSNTYPQEMSSCSLESDTF 383
           RLSN SEFE+FSPGP SP  + +G G  KS+D+N N+NY+  ++++PQ+MS CS  S+TF
Sbjct: 181 RLSNASEFEVFSPGPTSPLMLEMGEGTNKSVDSNTNDNYNDNTHSFPQDMSFCSSPSETF 240

Query: 384 SWTSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVE 443
           SW SHS  QDDVEAEMRRLKLELKQTMDMYSTACKEA+ AKQKA ELHRWK+EEEQR+  
Sbjct: 241 SWPSHSQTQDDVEAEMRRLKLELKQTMDMYSTACKEAITAKQKAMELHRWKVEEEQRLEA 300

Query: 444 ARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKAL-- 503
           ARMAEEAALA+AE EK+KCKAA+EAAEAAQ+ AALEA+KRA VEKM DMEA+E K+ L  
Sbjct: 301 ARMAEEAALAMAEKEKIKCKAAMEAAEAAQRNAALEAKKRARVEKMVDMEAQEMKRTLSF 360

Query: 504 AGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQ 563
           +G+G  +IGYR+YTIQ+IETAT GFSD+LKIGEGGYGPVFRGELDHT VAIKVLRPDAA 
Sbjct: 361 SGYGQAEIGYRKYTIQEIETATKGFSDSLKIGEGGYGPVFRGELDHTPVAIKVLRPDAAH 420

Query: 564 GRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQL 623
           GRSQFQQEVEVLSCIRHPNMVLLLGACPE  CLVYEFMANGSLEDCLFRK +DP+L WQL
Sbjct: 421 GRSQFQQEVEVLSCIRHPNMVLLLGACPEKGCLVYEFMANGSLEDCLFRKANDPILSWQL 480

Query: 624 RFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSV 683
           RFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLD NYVSKISDVGLARLVPPS+ADSV
Sbjct: 481 RFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDSNYVSKISDVGLARLVPPSVADSV 540

Query: 684 TQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEK 743
           TQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAV RAIEK
Sbjct: 541 TQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVERAIEK 600

Query: 744 GKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLG 803
           GKFAE+LDP+VKDWPVEDALKFAKLSI+CAEMRKRDRPDLGKVVLPELNRLR MAEES+G
Sbjct: 601 GKFAEILDPEVKDWPVEDALKFAKLSIKCAEMRKRDRPDLGKVVLPELNRLRTMAEESMG 660

Query: 804 IRPYSISSSSSSLCASQASSSQELISLHQGHYGSWVESSG----SSMSSYSGRRPSWLD 827
           +  +  SS+SSS  ASQ  +SQE+++  +   G+WVE+S     SS SSY+  +P W+D
Sbjct: 661 LGSFCCSSTSSSPYASQ--TSQEMMNSVRQQAGAWVETSSSQSDSSTSSYTREKPYWVD 713

BLAST of Tan0002319 vs. ExPASy TrEMBL
Match: A0A6J1KDI0 (U-box domain-containing protein 34-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492265 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 1.1e-257
Identity = 487/646 (75.39%), Postives = 540/646 (83.59%), Query Frame = 0

Query: 175 PAKPIAPSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPR 234
           PA+PIAP R+Q   A PRPQLLHQ TN S++ AE P Q  ++ S+ LETQ S      P 
Sbjct: 2   PARPIAPPRNQSLRASPRPQLLHQRTNVSQLLAE-PNQLWSKSSRRLETQSSM---RVPH 61

Query: 235 VIPDDLEIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDS 294
            +   L+ KSPFTR GSRA + K  DP T+DSD SFVS GRRSVDRGF  A +  +  DS
Sbjct: 62  ALQQYLDFKSPFTRQGSRAPVSKRVDPSTVDSDNSFVSCGRRSVDRGFSGALSYATCRDS 121

Query: 295 YRLSNGSEFEIFSPGPASPSPM----LGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLES 354
           YRLSN SE E  SPGP  PSP     +  G  KSIDNNN ++YS      Q++SSCS+ES
Sbjct: 122 YRLSNVSESETSSPGPPPPSPSPTLEIESGMNKSIDNNNFSSYS------QDLSSCSIES 181

Query: 355 DTFSWTSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQR 414
           DTF+  SHS+ QDDVEAEMRRLKLELKQTMDMYSTACKEALNA QKA ELHRWK+EEEQR
Sbjct: 182 DTFTRPSHSHAQDDVEAEMRRLKLELKQTMDMYSTACKEALNANQKATELHRWKVEEEQR 241

Query: 415 IVEARMAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKA 474
             EARMAEEAALA+AE EK+KC+AA++AAEAAQKIA LEARKR + E+MADMEAEETKKA
Sbjct: 242 FEEARMAEEAALAMAEREKMKCQAAIKAAEAAQKIAELEARKRENAERMADMEAEETKKA 301

Query: 475 LAGFGHGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAA 534
           L+GFGHG+IGYR+YTI++IE ATN FS++LKIGEGGYGPVFRG+LDHT VAIKVLRPDAA
Sbjct: 302 LSGFGHGEIGYRKYTIEEIEIATNHFSNSLKIGEGGYGPVFRGKLDHTPVAIKVLRPDAA 361

Query: 535 QGRSQFQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQ 594
            GRSQFQQEVEVLSCIRHPNMVLLLGACPEN CLVYEFM NGSLEDCLFRK ++PVL WQ
Sbjct: 362 HGRSQFQQEVEVLSCIRHPNMVLLLGACPENGCLVYEFMGNGSLEDCLFRKANNPVLSWQ 421

Query: 595 LRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADS 654
           LRFRIAAEIATGLLFLHQ RPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVP S+ADS
Sbjct: 422 LRFRIAAEIATGLLFLHQHRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPLSVADS 481

Query: 655 VTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIE 714
           VTQYRMTATAGTF YIDPEYQ TGLLGI+SDIYSLGVMLLQI+T RPPIGLAHAV RAI+
Sbjct: 482 VTQYRMTATAGTFCYIDPEYQLTGLLGIQSDIYSLGVMLLQIVTGRPPIGLAHAVERAIK 541

Query: 715 KGKFAEMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESL 774
           KGKFAEMLDP+VKDWP EDALKFAKLS++C EMRKRDRPDLGKVVLPELNRLRAMAEES+
Sbjct: 542 KGKFAEMLDPEVKDWPFEDALKFAKLSMKCTEMRKRDRPDLGKVVLPELNRLRAMAEESM 601

Query: 775 GIRPYSISSSSSSLCASQASSSQELISLHQGHYGSWVESSGSSMSS 817
            IR ++ SS+SSS   SQ SSSQE + +HQ   GSWVESS S  +S
Sbjct: 602 RIRAFNRSSASSSPYTSQTSSSQEPMRIHQEQCGSWVESSSSQSNS 637

BLAST of Tan0002319 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 774.2 bits (1998), Expect = 1.0e-223
Identity = 440/787 (55.91%), Postives = 555/787 (70.52%), Query Frame = 0

Query: 14  VAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPS--NSTTNV---SANFDSDEAA 73
           VAVAIDKDK SQ ALKW ++ LL RGQ + L+HVK +PS  N++ ++   SA    D + 
Sbjct: 20  VAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRPSPLNNSASLHASSAKLSQDSSL 79

Query: 74  AYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLGAP 133
             +         + FLPFRC CTR +I C +V+LE+ +++K L+ YVN+  I++LV+G+ 
Sbjct: 80  VCRD--PEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKALVEYVNQAAIEVLVVGSS 139

Query: 134 SRTSIVKRFK-SDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQLQ 193
           S+   ++  K +DIP  ++K APDFCTVY I +KGK+ ++R ++  AP    AP RS +Q
Sbjct: 140 SKGGFLRFNKPTDIPGSITKNAPDFCTVYII-SKGKIQTMRSASRSAPM--TAPLRSSVQ 199

Query: 194 PAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDLE-IKSPF 253
           P   +P      T+A+ + A+       Q     + Q  ++ +   R I D  E  +SPF
Sbjct: 200 PPSLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQRRSIDDQSESFRSPF 259

Query: 254 TRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAW-NSGSGGDSYRLSNGSEFEI 313
           TR G+  S G   D    +SDISF+SSGR S+DR FP  + N+       RLSN S+ + 
Sbjct: 260 TRRGNGRSYG---DLTVPESDISFISSGRPSIDRIFPSLYDNNDPSRTPPRLSNFSDMDY 319

Query: 314 FSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSNGQDDV 373
            S    S +      G +S+D N+  ++               ES+ FS  S S   DDV
Sbjct: 320 SSSLDQSSN-----YGRRSVDMNSPTDF---------------ESERFS--SASQSIDDV 379

Query: 374 EAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAEEAALAIA 433
           EAEMRRLKLELKQTM+MYSTACKEAL AKQKA EL RWKLEEE+++ EAR AEEAALAIA
Sbjct: 380 EAEMRRLKLELKQTMEMYSTACKEALTAKQKATELQRWKLEEERKLEEARNAEEAALAIA 439

Query: 434 ENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGDIGYRRYT 493
           E EK K KAA+EAAEAAQ+IA LEA+KR + E  A  E+EE  KAL    + D+ YR+Y+
Sbjct: 440 EKEKAKSKAAMEAAEAAQRIAELEAKKRVNAEMKALKESEEKTKALTALANSDVRYRKYS 499

Query: 494 IQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSC 553
           I+DIE AT  F++  KIGEGGYGPV++  LDHT VA+KVLRPDAAQGRSQFQQEVEVLSC
Sbjct: 500 IEDIELATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSC 559

Query: 554 IRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAEIATGLLF 613
           IRHPNMVLLLGACPE  CLVYEFMANGSLED LFR  + P L WQ+RFRIAAEI TGLLF
Sbjct: 560 IRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLF 619

Query: 614 LHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTATAGTFFY 673
           LHQ +PEP+VHRDLKPGNILLDRN+VSKISDVGLARLVPP++AD+VTQYRMT+TAGTF Y
Sbjct: 620 LHQAKPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCY 679

Query: 674 IDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFAEMLDPDVKDW 733
           IDPEYQQTG+LG+KSDIYSLG+M LQ+ITA+PP+GL H V RA+EKG   ++LDP V DW
Sbjct: 680 IDPEYQQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHYVERALEKGTLVDLLDPVVSDW 739

Query: 734 PVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPYSISSSSSSLC 793
           P+ED  +FAKL+++CAE+R++DRPDL KV+LPELNRLR +A+ES     +S+   +S L 
Sbjct: 740 PMEDTEEFAKLALKCAELRRKDRPDLAKVILPELNRLRTLADES----SHSVVVGNSPLP 772

BLAST of Tan0002319 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 718.0 bits (1852), Expect = 8.7e-207
Identity = 417/789 (52.85%), Postives = 525/789 (66.54%), Query Frame = 0

Query: 14  VAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSANFDSDEAAAYKHQ 73
           VAVAID+DK SQ ALKW ++ LL +GQ + L+HVK + S+ +TN S N +S + +     
Sbjct: 18  VAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINSNSSKTSQINGD 77

Query: 74  LD------NHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLGA 133
                     +  Q FLPFRC C+R +I C +V+LE+ ++++ L+ Y N+ +I++LV+G+
Sbjct: 78  SSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYANQVMIEVLVVGS 137

Query: 134 PSRTSIVKRFKS-DIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQL 193
            S+   ++  K  DIP  ++K APDFCTVY I  KGK+S+ + ++  AP+  ++P R QL
Sbjct: 138 SSKGGFLRFNKPIDIPGAITKTAPDFCTVYAI-TKGKLSTKKTASRAAPS--VSPLRIQL 197

Query: 194 QP--APPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDLEIKS 253
           Q     P P L    TN          Q R  L       D + S  PP           
Sbjct: 198 QQNGLKPHPPLPSATTNTRAERQSFESQHRRSL------DDQSDSFRPPY---------- 257

Query: 254 PFTRPGSRASLGKGQDPFTL-DSDISFVSSGRRSVDRGFPPAW-NSGSGGDSYRLSNGSE 313
                  R   G+     ++ DS+ISF SSGR S++R  P  + NS       RLSN S+
Sbjct: 258 -----NKRGLTGRSYGELSIPDSEISFNSSGRPSIERNSPSLYDNSDPNRTPPRLSNFSD 317

Query: 314 FEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSNGQ 373
            +                   S ++      S   + P   S+ S E++ FS  S S G 
Sbjct: 318 VDYC-----------------SFESMTFGRRSMDLSSPTAFSTGSFENERFS--SASQGG 377

Query: 374 DDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAEEAAL 433
           DDVEAEMRRLKLELKQTM+MYSTACKEAL AK KA EL RWKL EE++  EA++AEEAAL
Sbjct: 378 DDVEAEMRRLKLELKQTMEMYSTACKEALTAKHKATELQRWKLAEERKFEEAKLAEEAAL 437

Query: 434 AIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGDIGYR 493
           AIAE EK K KAA+EAAEAAQ+IA +E+RKR   E  A  E+E   KA+      D+ YR
Sbjct: 438 AIAEKEKAKSKAAMEAAEAAQRIADIESRKRVDAETKALKESEARTKAVNALAK-DVRYR 497

Query: 494 RYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQEVEV 553
           +Y+I++IE AT  F D  KIGEG YGPV++  LDHT VA+K LRPDAAQGRSQFQ+EVEV
Sbjct: 498 KYSIEEIEDATEFFDDKYKIGEGSYGPVYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEV 557

Query: 554 LSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAEIATG 613
           L  IRHPNMVLLLGACPE  CLVYEFMANGSLED LFR+ D P L WQ RFRIAAEI T 
Sbjct: 558 LCSIRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTV 617

Query: 614 LLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTATAGT 673
           LLFLHQT+PEP+VHRDLKP NILLDRN+VSK++DVGLARLVPPS+A++VTQY MT+TAGT
Sbjct: 618 LLFLHQTKPEPLVHRDLKPANILLDRNFVSKLADVGLARLVPPSVANTVTQYHMTSTAGT 677

Query: 674 FFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFAEMLDPDV 733
           F YIDPEYQQTG+LG+KSDIYSLG+M LQ+IT +PP+GL H V RA+EKG   ++LDP V
Sbjct: 678 FCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITGKPPMGLTHYVERALEKGNLKDLLDPAV 737

Query: 734 KDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPYSISSSSS 792
            DWPVED  +FAKL+++CAE+R++DRPDL KV+LPELNRLR +AEES       I+SS  
Sbjct: 738 SDWPVEDTTEFAKLALKCAEIRRKDRPDLSKVILPELNRLRVLAEESTQ-AAVVINSSGP 761

BLAST of Tan0002319 vs. TAIR 10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 708.0 bits (1826), Expect = 9.0e-204
Identity = 415/823 (50.43%), Postives = 537/823 (65.25%), Query Frame = 0

Query: 7   AQKKEESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSANFDSDE 66
           ++K+EE++ +AID+DK SQ ALKW +  LLSRGQ LTLLHVK K  +S     +NF    
Sbjct: 5   SEKREENITLAIDRDKESQNALKWAVSNLLSRGQTLTLLHVKLKQPSSLPYSGSNFSK-- 64

Query: 67  AAAYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLG 126
                         + FLPFRC C R +I C +V++ED   +K +++YV +N I+ L+LG
Sbjct: 65  --------PGDDPSELFLPFRCYCARKDINCQDVVVEDVSAAKGIVDYVQQNAIETLILG 124

Query: 127 APSRTSIVKRFK-SDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQ 186
           +   T +  RFK +D+ S V K AP FCTVY I +KGK+S LR +T+  P          
Sbjct: 125 SSKMTLL--RFKAADVSSTVMKKAPSFCTVYVI-SKGKISFLRSATSSPP---------- 184

Query: 187 LQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIPDDLEIKSP 246
                                  H   P  +   + +T +  +      +     EIK  
Sbjct: 185 -----------------------HSNMPSMRHHSHAQTSNMNVERRQQTMQRTHDEIKIE 244

Query: 247 FTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNGSEFEI 306
             R            P   DSDISFVSSGR SVD+ FP  ++     D  RLS  SE+  
Sbjct: 245 IKRGYEGV-----YQPSITDSDISFVSSGRPSVDQMFPSLYDD---VDVPRLSVTSEY-- 304

Query: 307 FSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEM----------SSCSLESDTFSW 366
                                    N  SF+ TY ++           SS S ES   S+
Sbjct: 305 -----------------------GENRLSFATTYSKQSIDLGSPYAPNSSTSFESGRQSF 364

Query: 367 TSHSNGQDDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEAR 426
           +    GQD++E EMRRLK+ELK TM+MY++ACKEA++AK+ A EL +WK E+E ++ E R
Sbjct: 365 S--LQGQDELETEMRRLKMELKHTMEMYNSACKEAISAKKAANELLKWKAEKEHKLEEVR 424

Query: 427 MAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFG 486
           +++EAA+A+AE EK K +AA+EAA AAQK++ LEA KR  +E +     +E K+A++   
Sbjct: 425 LSKEAAMAMAEREKEKSRAAMEAAVAAQKLSDLEAEKRKHIETV-----DEKKRAVS--- 484

Query: 487 HGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQ 546
              + YR+YTI++IE AT  FS + K+GEGGYGPV++G LD+T+VAIKVLRPDAAQGRSQ
Sbjct: 485 --SLRYRKYTIEEIEEATEDFSPSRKVGEGGYGPVYKGTLDYTKVAIKVLRPDAAQGRSQ 544

Query: 547 FQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRI 606
           FQ+EVEVL+C+RHPNMVLLLGACPE  CLVYE+MANGSL+DCLFR+ + P+L WQLRFRI
Sbjct: 545 FQREVEVLTCMRHPNMVLLLGACPEYGCLVYEYMANGSLDDCLFRRGNSPILSWQLRFRI 604

Query: 607 AAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYR 666
           A+EIATGL FLHQ +PEP+VHRDLKPGNILLD+++VSKISDVGLARLVPPS+AD+ TQYR
Sbjct: 605 ASEIATGLHFLHQMKPEPLVHRDLKPGNILLDQHFVSKISDVGLARLVPPSVADTATQYR 664

Query: 667 MTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFA 726
           MT+TAGTFFYIDPEYQQTG+LG KSDIYS G+MLLQI+TA+PP+GL H V +AIEKG FA
Sbjct: 665 MTSTAGTFFYIDPEYQQTGMLGTKSDIYSFGIMLLQILTAKPPMGLTHHVEKAIEKGTFA 724

Query: 727 EMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESL--GIR 786
           EMLDP V DWP E+AL  AKL++QCA++R++DRPDLG +VLPEL +LR +AEES+  G+R
Sbjct: 725 EMLDPAVPDWPFEEALAAAKLALQCAKLRRKDRPDLGNIVLPELKKLRDLAEESIKFGVR 731

Query: 787 PYSISSSSSSLCASQASSSQELISLHQGHYGSWVESSGSSMSS 817
             S   SS S     A+S QE+IS  Q  YGS   S  +S +S
Sbjct: 785 QPSPIRSSGS-----ATSIQEIISDPQLQYGSDSSSLHNSSTS 731

BLAST of Tan0002319 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 696.8 bits (1797), Expect = 2.1e-200
Identity = 400/775 (51.61%), Postives = 521/775 (67.23%), Query Frame = 0

Query: 1   MARGGGAQKK--EESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNV 60
           + +  GA+K+    SVAVAIDKDK SQ ALKWTI+ L SRGQ ++L+HV  +  +S+   
Sbjct: 3   LPKANGAKKETGSGSVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCRSHSSS--- 62

Query: 61  SANFDSDEAA-AYKHQLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNK 120
               D +E     K Q++  A+D  F+ F C C+R  I C +++LED +  + +  YV+ 
Sbjct: 63  ----DLEEGTPQQKQQMEKIAKD-LFVSFHCYCSRKEINCRDILLEDADKVRAITEYVSS 122

Query: 121 NVIDILVLGAPSRTSIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAK 180
           + I+ LV+G+ SR   ++RFK+D+P+ VSK APDFC VY I +KGK++S+R ++ PAP +
Sbjct: 123 SAIENLVVGSASRNGFMRRFKTDLPTTVSKSAPDFCNVYVI-SKGKIASVRNASRPAPYQ 182

Query: 181 PIAPSRSQLQPAPPRPQLLHQLTNASEVAAEHPYQPRTQLSKYLETQDSTISNPPPRVIP 240
                       P  P    +  +    A   P +PR    K +E   S +    P    
Sbjct: 183 NSMQQCEIDNHHPHTPDKAPKYHDHPNSAGSTPSRPR----KSVEADRSPLVKRKPYGDF 242

Query: 241 DDLEIKSPFTRPGSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRL 300
            D +    F  P S             DSDISF+SSGR SV+R                 
Sbjct: 243 YDSDSDLSFISPSSHR-----------DSDISFISSGRPSVER----------------- 302

Query: 301 SNGSEFEIFSPGPASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTS 360
              S F +  P  A  S M     T S  +  +N      + P  ++  S  S+    TS
Sbjct: 303 ---SSFSLDFPESARTSRM----STSSEQSIGSNRLGIKFSDPDFLNESSTFSEESGRTS 362

Query: 361 HSNGQ--DDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEAR 420
             + Q  DDVEAEM+RL+LELKQTMDMYSTACKEAL+A+Q+A EL + + EEE+R+ EA+
Sbjct: 363 SYSSQSLDDVEAEMKRLRLELKQTMDMYSTACKEALSARQQATELQKLRTEEERRLEEAK 422

Query: 421 MAEEAALAIAENEKLKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFG 480
            +EEAA++I E E+ K KAALEAAEAA+++A +E+++R + E        +T K    F 
Sbjct: 423 SSEEAAMSIVEKERAKAKAALEAAEAAKRLAEVESKRRLTAEM-------KTMKESDSFS 482

Query: 481 HGDIGYRRYTIQDIETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQ 540
            G + YR+YT+ +IE AT+ F+++ K+GEGGYGPVFRG LDHT VA+KVLRPDAAQGRSQ
Sbjct: 483 RGFVRYRKYTVDEIEEATSNFAESQKVGEGGYGPVFRGFLDHTSVAVKVLRPDAAQGRSQ 542

Query: 541 FQQEVEVLSCIRHPNMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRI 600
           FQ+EVEVLSCIRHPNMVLLLGACPE   LVYE+MA GSLED LF + + P + WQLRFRI
Sbjct: 543 FQKEVEVLSCIRHPNMVLLLGACPEFGILVYEYMAKGSLEDRLFMRGNTPPITWQLRFRI 602

Query: 601 AAEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYR 660
           AAEIATGLLFLHQT+PEPIVHRDLKPGN+LLD NYVSKISDVGLARLV P++A++VTQYR
Sbjct: 603 AAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDYNYVSKISDVGLARLV-PAVAENVTQYR 662

Query: 661 MTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFA 720
           +T+ AGTF YIDPEYQQTG+LG+KSD+YSLG+MLLQI+TA+ P+GLA+ V +AIE+G   
Sbjct: 663 VTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIMLLQILTAKQPMGLAYYVEQAIEEGTLK 721

Query: 721 EMLDPDVKDWPVEDALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESL 771
           +MLDP V DWP+E+AL  AKLS+QCAE+R++DRPDLGK +LPELNRLR + EESL
Sbjct: 723 DMLDPAVPDWPIEEALSLAKLSLQCAELRRKDRPDLGKEILPELNRLREIGEESL 721

BLAST of Tan0002319 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 687.6 bits (1773), Expect = 1.3e-197
Identity = 393/773 (50.84%), Postives = 514/773 (66.49%), Query Frame = 0

Query: 13  SVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKQKPSNSTTNVSANFDSDEAAAYKH 72
           SVA+AIDKDK SQ A+KWT+E L +RGQ L L+HV  K  +S        D +E   +K 
Sbjct: 17  SVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHVLPKSQSS-------LDIEEGITHKQ 76

Query: 73  QLDNHARDQFFLPFRCACTRSNIPCNEVMLEDGEISKTLINYVNKNVIDILVLGAPSRTS 132
           Q++   +D  F+ F C C+R  I C +V+LED +  K ++ YV  + I+ LVLGAPSR S
Sbjct: 77  QMEKQTKD-LFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIENLVLGAPSRNS 136

Query: 133 IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSLRQSTAPAPAKPIAPSRSQLQPAPPR 192
            ++RFK+D+P+ VSK APDFC VY I +KGK+SSLR ++               + AP  
Sbjct: 137 FMRRFKTDLPTSVSKAAPDFCNVYVI-SKGKISSLRNAS---------------RLAPYH 196

Query: 193 PQLLHQLTNASEVAAE--HPYQPRTQLSKYLETQDSTISN-PPPRVIPDDLEIKSPFTRP 252
           P +L ++ +   +A E  H       L K   + DS ++    P+     +++   F+  
Sbjct: 197 PSVLSEVDDHETIAIERKHKTANTPALPKGRRSIDSDVTRLGLPKPPHGHMKLMGDFSDS 256

Query: 253 GSRASLGKGQDPFTLDSDISFVSSGRRSVDRGFPPAWNSGSGGDSYRLSNGSEFEIFSPG 312
            S  S        +  SD+SF+SSGR SVDR                    S F    P 
Sbjct: 257 ESEFSFISASQQES--SDLSFISSGRPSVDR--------------------SSFTYDLPE 316

Query: 313 PASPSPMLGMGGTKSIDNNNNNNYSFSNTYPQEMSSCSLESDTFSWTSHSNGQDDVEAEM 372
            A  S M      +SI ++         +Y    SS S ES   S +  S   +DVEA+M
Sbjct: 317 SARTSRM-STSSEQSIGSHRLGIKFTDLSYLNGSSSVSDESGRTSCSFSSQSLNDVEAQM 376

Query: 373 RRLKLELKQTMDMYSTACKEALNAKQKAKELHRWKLEEEQRIVEARMAEEAALAIAENEK 432
           +RL+LELKQTMDMYS+AC+EAL A+ +A EL + + EEE+R+ E +M EE A++I ENE+
Sbjct: 377 KRLRLELKQTMDMYSSACREALTARNEATELQKLRTEEERRLEELKMTEETAMSIVENER 436

Query: 433 LKCKAALEAAEAAQKIAALEARKRASVEKMADMEAEETKKALAGFGHGDIGYRRYTIQDI 492
            K K ALEAAEAA ++A +EA++R   E     E++   +      H  + YR+Y++Q+I
Sbjct: 437 AKAKTALEAAEAANRLAEVEAKRRVHAEMKVLKESDSFSR------HSIVRYRKYSVQEI 496

Query: 493 ETATNGFSDALKIGEGGYGPVFRGELDHTQVAIKVLRPDAAQGRSQFQQEVEVLSCIRHP 552
           E  T  F+++ K+GEGGYGPVFRG LDHT VA+KVLRPDAAQGRSQF +EVEVLSCIRHP
Sbjct: 497 EEGTANFAESRKVGEGGYGPVFRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLSCIRHP 556

Query: 553 NMVLLLGACPENACLVYEFMANGSLEDCLFRKPDDPVLPWQLRFRIAAEIATGLLFLHQT 612
           NMVLLLGACPE   LVYE+MA GSL+D LFR+ + P + WQLRFRIAAEIATGLLFLHQT
Sbjct: 557 NMVLLLGACPEYGILVYEYMARGSLDDRLFRRGNTPPISWQLRFRIAAEIATGLLFLHQT 616

Query: 613 RPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSMADSVTQYRMTATAGTFFYIDPE 672
           +PEPIVHRDLKPGN+LLD NYVSKISDVGLARLV P++A++VTQYR+T+ AGTF YIDPE
Sbjct: 617 KPEPIVHRDLKPGNVLLDHNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPE 676

Query: 673 YQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVGRAIEKGKFAEMLDPDVKDWPVED 732
           YQQTG+LG+KSD+YSLG+MLLQ++TA+ P+GLA+ V +AIE+G   +MLDP V DWP+E+
Sbjct: 677 YQQTGMLGVKSDVYSLGIMLLQLLTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPLEE 735

Query: 733 ALKFAKLSIQCAEMRKRDRPDLGKVVLPELNRLRAMAEESLGIRPYSISSSSS 783
           AL  AKLS+QCAE+R++DRPDLGK V+PEL+RLR + EESL    Y+    SS
Sbjct: 737 ALSLAKLSLQCAELRRKDRPDLGKEVMPELSRLREIGEESLDSVYYAGQGRSS 735