Tan0002172 (gene) Snake gourd v1

Overview
NameTan0002172
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectin lyase-like superfamily protein
LocationLG10: 9314439 .. 9318795 (+)
RNA-Seq ExpressionTan0002172
SyntenyTan0002172
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAAACAATTTTGTCGGCGAGAGACGTTATTAATCATATTCACAAAGAGGGTAATTGCCAATTAGTGGCAATTAATATTCTTATTAATCAATTTTTTTTTTCCATTTTATTCTCCTTTGCAGCAACAACAAGGCAGAATTTCTTCACCCAATTTCTTCTTTCTTTCTCTCTTTTTTTTTTTTGGGTGGATTTGTTTTTTTGTTTTTCTTCTTCTTCTCTCTCTGTCTTTGTGTGTGTGTGTGTGCATTTGGAAAGCTGTCATTTCTACATCGTATACTGTCCTTCCTTTCTTCCTTCCTTCCTTCCTTTCCCTACAATTTCCTTTTGTAAGTTATTGATTTTGGCTATGCATTTATATCATTAATGCTTATTATCGAATCCCCATTTGCTGCCTCACTCTCACTTTCTTTGCCTCTGTTTGTTTCTTTTTGTTTTTCCGGCTTCAACTTCACCTCCAGCTTCCTCTCTGCCCTCGCCGATTTTGCTGCTGTTTTCCCTCCTTCTCCTTCGGTCTTTTGGATTCTGCATCTCGTTTTCTTTTCTTTTTGGTCATTGTCGTCTCTGGCTTTGACCAATGAGGATGCTAGTAAGTACGTTTCTTTTTATCTGTTTTTTTTTTTTTTTTTTTTTTCTCATTACATACTACTTGATTCTTTTGTTCTCTCTAGTTTGTTTCCCGAATCTTTTGCACCCATATGCTAATTTTCTGTTGATTTCTGGAGAAACCCCTTTTCGTTTTTTTGATGGGTTTTTTTTTTTTTGGGTTCCTACCCTTGTTTTCAATGTTTTTCAGCATCTGTTTACCTCGGGTTCTTTCTTTTCGCTCTGTGTTATCTATGTTTCTTTACAGATGGTCCAGAAACCCTTTTTGACTTTTCGTGATTTGTATTCCTTCTCTTCCCCTATTGACCTTTTTCTCTCTCCTTGAACATTTCCTTGATGTTGATTTTCTGGCAGGTTTCAATTTCCAGATTTGATGTTCTTCTGCATTTTGTTAGTTTCACGCCTTTGATTTCTCAATTTTATGCTTCAATTTCTCTTTGATCTGCTGTTTCTTCTTGTGGGATTAGTTTCTTACTTCTGTTGGTGGTTTTTTTTTATATCTCCTGATTTGCAATTTCTAGCATCGATGCCTTCATTTTTTCATCCGCTCTCTGCTTGAATCTGCAATTTTGGGCGATTTGTAAACTTGTTCCTCAATGCACTATTGCTTCTATTAGATTTCGGCCTTGCTGGTTTCTACCATTGTTGTCTTTTTTATCTGTTTTTGTTGGTTGATTTAATCAAATTCATGGTGTTGCTTCTAAGTTTCTTTTGGTTCTTTCTTTAGGTGGCATTGCTGTTGTTGCTACAATTGAGCAATGCTGCAAAAGTGATCGGTGAAGAGGTCGCCGGACAGTGCGATTCTAAACCAACATTAGATCCAAGACCACACAGTGTGTCTATCTTGGAGTTTGGGGCTGTTGGAGATGGCAAAACTCTCAACACCATTGCCTTCCAAAATGCCATTTTCTATCTCAAATCCTTTGCTGATAAAGGTGGGGCGCAGCTTTATGTTCCACCTGGAAAATGGCTCACTGGAAGCATTAATCTTACCAGCCACCTTACCCTCTTCTTGGAAAAAGGTGCCACAATTCTTGGCTCTCAGGTATGCTCTTCTTCTTGGATGGACAAAGTGATGCTCAAACAAGTTGCTGTTGCTAGGCATTTGTTTTGTAACATATTGATCTTCTCTTAAACAGGATCCATCACATTGGGAACTTGTTAATCCCTTACCTTCGTATGGTAGAGGTATTGAAGTTCCAGGGAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTTTGTCATTCTTCTAAATCCTTGATTTAGAATCTAGTTTGTGGAAATGTGATTCCATCCTCTGAACAAGTGGTTATTATGTTGATTTTTGATGTGCTAGGTGATGATGGGGTCATTGATGGACAGGGTTTAGTTTGGTGGGATTGGTTCAGCTCTCATTCCTTAAACTACAGCCGCCCTCACCTCGTGGAATTTGAGGATTCTAAATATGTAGTTGTTTCCAACCTCACTTTCTTGAATACTCCTGCATATAACATCCATCCAGTCTATTGCAGGTATGCTATTGCAACTAGGGTTCCGTTTCTTCTGCCTCAGCTGGTTCTGTTCATCCTGTTGATATGTTTATATGTTGATTGTGAGAGAATCTGTATTGAGATAAGTTTTTTCATGTGCAGTAATGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGGTTTGTAATGTTTCTTCAGCTTCCTCATTGTCATTTTGTGTGACTATTGGCGGTGGGTTCTCTCTCCCTCTCTCTCCCCCATAAGATGTGAGACATCGTCTGGCTGAGTGGCATGAGCACCATTGAAGAATCTCTCCCTTTATCTCTCTCTCTGTCTCTCTCCCACCCACCCACCCACCCACACACACCACATAGTGATCAGGAGAAGATGCCATCAAGAAAGCTATCAAGAGTAGTTGTCTGCTAACAGATATTTTATAAACTTTAGTTAATATCAACACCTAGTCATGATGGACAATTTTGCTAAATGTCTTATGCTTGTTTAGCATCATGTAATCTAATTAAGAAAACCGATGTCTTCTAATGAAGTCCCAATTCACGAAGTTCTTATGTGTCAGGTCTAAGCTCTATGGTGATAACTTTTCACAGTAAAATTTAAGAAACTTAGCCTGGTAAAATGGTGAAGTTGGATTGAATTTATAAAATAAGACTCTGTTAGGAGTTGGTGACACGTAGTGGGATACCATCATGATCAAAAGGATTTATTGTGGGACAAAATTTAGATATACATTACATGGATATCTAATGATGATCAGCCTCAGAATTCAAGTTTAGAAGTAGTATGCATCTCATTCTTTCTTGTATGTAATTAATAAGGTGAAGAAGCATTCTTTTCTAAATAAAGTTTGATTGGTGCTTCCAGATTCTTCTGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGGAGACCATCTAAAAACATACACATCAGAAGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAAATGTCTGGTGGGATCTCTAATGTTCTTGTGGAGCATGTGCACCTGTACAACTCATCTACTGGCATTCAATTTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTCATTTCAGATGTTGAAATGGAAAACATATCCACAGGATTCAGTGCCTCAGGTCACTTCGGGTCACATCCGGATGACGAGTTTGATCCTAATGCACTTCCAATTGTGCAGGACATAACCTTGCAGAATGTGAGAGGCACGAACATTGAAATTGCTGGAAACTTTACTGGCATCCAGGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATTTGTTCAGATGTTTCTGGCTATTCAGAATCTGTGATCCCACCACCCTGTTCTGATCTCAATACTCCATATTCAATTTCTTCCTCAGCAGCCTCTTCCCTTCTGAATTCAAATGGAAAAACTGCTGTTTTATGAAAATCCTTTCCATCCAAGGTACAACAATATATATTTCAACCCCCATTCTAAATGAGGGAAAAATACCAAAACTTTCAACCATTGCCTTCTCCAAGTCAAGCCAGATTCTTTCAAATAAAAACCACATGTCTAGATTACTTGTTTCTTCATTCTCTTAGATTTGCTTTCTTCCATGTTGCGGTCTGCAGATTTATTTGTACAGTTCCATTTCTTCATTCACGTGTAAGGAAATTTTTGGTAGTGATTTTACAGGTCTTTCGGTATCGTTTGTGTTGCTTTTTTAAATTATTCCCCCCTCCCTCTTCCCCCTCAAGAGCTTGGTGCATTGTAATTTCACTGTAAATTGTTCATTTGTTGTGAAGGGCTGAAAGGATAAAACTCTAGTTGATGTGAAAAGCAATTCATGTGGAGCCAAGTGATTGGAATAAAGGGATCTTGGTGACACTCTGCTGCTTTAAGTAAATTAAGCTGGGTGTTTTTTTTTTTTTTTTCAATTCAAGTATACATTTCACTTCTCTTCCTCTTCCTGTTATATTTAGTAATGGATCATCCTTTCCGGTCATTTCCTTCCATTGTGTTTTTTTTTTTAATCTGATGCATGAATGGCTACTATTCTGACAACCATCATGAAATGTGATGCAAACAGGCT

mRNA sequence

TTTCAAACAATTTTGTCGGCGAGAGACGTTATTAATCATATTCACAAAGAGGGTAATTGCCAATTAGTGGCAATTAATATTCTTATTAATCAATTTTTTTTTTCCATTTTATTCTCCTTTGCAGCAACAACAAGGCAGAATTTCTTCACCCAATTTCTTCTTTCTTTCTCTCTTTTTTTTTTTTGGGTGGATTTGTTTTTTTGTTTTTCTTCTTCTTCTCTCTCTGTCTTTGTGTGTGTGTGTGTGCATTTGGAAAGCTGTCATTTCTACATCGTATACTGTCCTTCCTTTCTTCCTTCCTTCCTTCCTTTCCCTACAATTTCCTTTTCTTCCTCTCTGCCCTCGCCGATTTTGCTGCTGTTTTCCCTCCTTCTCCTTCGGTCTTTTGGATTCTGCATCTCGTTTTCTTTTCTTTTTGGTCATTGTCGTCTCTGGCTTTGACCAATGAGGATGCTAGTGGCATTGCTGTTGTTGCTACAATTGAGCAATGCTGCAAAAGTGATCGGTGAAGAGGTCGCCGGACAGTGCGATTCTAAACCAACATTAGATCCAAGACCACACAGTGTGTCTATCTTGGAGTTTGGGGCTGTTGGAGATGGCAAAACTCTCAACACCATTGCCTTCCAAAATGCCATTTTCTATCTCAAATCCTTTGCTGATAAAGGTGGGGCGCAGCTTTATGTTCCACCTGGAAAATGGCTCACTGGAAGCATTAATCTTACCAGCCACCTTACCCTCTTCTTGGAAAAAGGTGCCACAATTCTTGGCTCTCAGGATCCATCACATTGGGAACTTGTTAATCCCTTACCTTCGTATGGTAGAGGTATTGAAGTTCCAGGGAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTGATGATGGGGTCATTGATGGACAGGGTTTAGTTTGGTGGGATTGGTTCAGCTCTCATTCCTTAAACTACAGCCGCCCTCACCTCGTGGAATTTGAGGATTCTAAATATGTAGTTGTTTCCAACCTCACTTTCTTGAATACTCCTGCATATAACATCCATCCAGTCTATTGCAGTAATGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGATTCTTCTGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGGAGACCATCTAAAAACATACACATCAGAAGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAAATGTCTGGTGGGATCTCTAATGTTCTTGTGGAGCATGTGCACCTGTACAACTCATCTACTGGCATTCAATTTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTCATTTCAGATGTTGAAATGGAAAACATATCCACAGGATTCAGTGCCTCAGGTCACTTCGGGTCACATCCGGATGACGAGTTTGATCCTAATGCACTTCCAATTGTGCAGGACATAACCTTGCAGAATGTGAGAGGCACGAACATTGAAATTGCTGGAAACTTTACTGGCATCCAGGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATTTGTTCAGATGTTTCTGGCTATTCAGAATCTGTGATCCCACCACCCTGTTCTGATCTCAATACTCCATATTCAATTTCTTCCTCAGCAGCCTCTTCCCTTCTGAATTCAAATGGAAAAACTGCTGTTTTATGAAAATCCTTTCCATCCAAGGTACAACAATATATATTTCAACCCCCATTCTAAATGAGGGAAAAATACCAAAACTTTCAACCATTGCCTTCTCCAAGTCAAGCCAGATTCTTTCAAATAAAAACCACATGTCTAGATTACTTGTTTCTTCATTCTCTTAGATTTGCTTTCTTCCATGTTGCGGTCTGCAGATTTATTTGTACAGTTCCATTTCTTCATTCACGTGTAAGGAAATTTTTGGTAGTGATTTTACAGGTCTTTCGGTATCGTTTGTGTTGCTTTTTTAAATTATTCCCCCCTCCCTCTTCCCCCTCAAGAGCTTGGTGCATTGTAATTTCACTGTAAATTGTTCATTTGTTGTGAAGGGCTGAAAGGATAAAACTCTAGTTGATGTGAAAAGCAATTCATGTGGAGCCAAGTGATTGGAATAAAGGGATCTTGGTGACACTCTGCTGCTTTAAGTAAATTAAGCTGGGTGTTTTTTTTTTTTTTTTCAATTCAAGTATACATTTCACTTCTCTTCCTCTTCCTGTTATATTTAGTAATGGATCATCCTTTCCGGTCATTTCCTTCCATTGTGTTTTTTTTTTTAATCTGATGCATGAATGGCTACTATTCTGACAACCATCATGAAATGTGATGCAAACAGGCT

Coding sequence (CDS)

ATGAGGATGCTAGTGGCATTGCTGTTGTTGCTACAATTGAGCAATGCTGCAAAAGTGATCGGTGAAGAGGTCGCCGGACAGTGCGATTCTAAACCAACATTAGATCCAAGACCACACAGTGTGTCTATCTTGGAGTTTGGGGCTGTTGGAGATGGCAAAACTCTCAACACCATTGCCTTCCAAAATGCCATTTTCTATCTCAAATCCTTTGCTGATAAAGGTGGGGCGCAGCTTTATGTTCCACCTGGAAAATGGCTCACTGGAAGCATTAATCTTACCAGCCACCTTACCCTCTTCTTGGAAAAAGGTGCCACAATTCTTGGCTCTCAGGATCCATCACATTGGGAACTTGTTAATCCCTTACCTTCGTATGGTAGAGGTATTGAAGTTCCAGGGAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTGATGATGGGGTCATTGATGGACAGGGTTTAGTTTGGTGGGATTGGTTCAGCTCTCATTCCTTAAACTACAGCCGCCCTCACCTCGTGGAATTTGAGGATTCTAAATATGTAGTTGTTTCCAACCTCACTTTCTTGAATACTCCTGCATATAACATCCATCCAGTCTATTGCAGTAATGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGATTCTTCTGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGGAGACCATCTAAAAACATACACATCAGAAGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAAATGTCTGGTGGGATCTCTAATGTTCTTGTGGAGCATGTGCACCTGTACAACTCATCTACTGGCATTCAATTTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTCATTTCAGATGTTGAAATGGAAAACATATCCACAGGATTCAGTGCCTCAGGTCACTTCGGGTCACATCCGGATGACGAGTTTGATCCTAATGCACTTCCAATTGTGCAGGACATAACCTTGCAGAATGTGAGAGGCACGAACATTGAAATTGCTGGAAACTTTACTGGCATCCAGGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATTTGTTCAGATGTTTCTGGCTATTCAGAATCTGTGATCCCACCACCCTGTTCTGATCTCAATACTCCATATTCAATTTCTTCCTCAGCAGCCTCTTCCCTTCTGAATTCAAATGGAAAAACTGCTGTTTTATGA

Protein sequence

MRMLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL
Homology
BLAST of Tan0002172 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.0e-112
Identity = 203/431 (47.10%), Postives = 277/431 (64.27%), Query Frame = 0

Query: 3   MLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 62
           +LVA++LLL +S      G  +  +      +  R HS S+++FG VGDG+TLNT AFQ+
Sbjct: 27  VLVAVVLLLSVSRGECRKGRIL--EALEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQD 86

Query: 63  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLP 122
           A+  L  +  +GGAQLYVP GKWLTGS +LTSH TLFL + A +L SQD S W ++ PLP
Sbjct: 87  AVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIKPLP 146

Query: 123 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEF 182
           SYGRG +    RY SLI G NL DV+ITGD+G IDGQG +WW  F    L Y+RP+L+E 
Sbjct: 147 SYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIEL 206

Query: 183 EDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 242
             S  + +SNLT LN+P++N+HPVY  N+ +  I++ AP  SP T GI PDS  +  IE 
Sbjct: 207 MYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRIED 266

Query: 243 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 302
           C I +G D +A+KSGWD+YGIAYG P+K + IRR+   S   + IA GSEMSGGI +V  
Sbjct: 267 CYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRA 326

Query: 303 EHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNAL 362
           E +   NS +GI+ +T  GRGGY+K I +  + M+ +   F  +G++GSH D+ +DP A 
Sbjct: 327 EDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAF 386

Query: 363 PIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINS-SSSTSWICSDVSGYS 422
           P++Q I  +++   N+ +A    GI   PFT I +SN+T  + + +    W C+DV G S
Sbjct: 387 PVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGIS 446

Query: 423 ESVIPPPCSDL 433
             V P PCS L
Sbjct: 447 SGVTPTPCSTL 455

BLAST of Tan0002172 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 2.1e-26
Identity = 110/404 (27.23%), Postives = 189/404 (46.78%), Query Frame = 0

Query: 38  PHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGK-WLTGSINLTS-- 97
           P +VS+ +FGA GDGKT +T AF NA  + K+ +  G   L VP G  +L  SI LT   
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124

Query: 98  HLTLFLEKGATILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDG 157
           +  L ++   T+  SQ  S ++ ++      + I   G      +N  ++      GD G
Sbjct: 125 NSILTVQIFGTLSASQKRSDYKDIS------KWIMFDG------VNNLSVDG----GDTG 184

Query: 158 VIDGQGLVWWDWFSSHSLNYSR-----PHLVEFEDSKYVVVSNLTFLNTPAYNIHPVYCS 217
           V+DG G  WW   +S   N ++     P  + F +SK ++V NL   N     I    CS
Sbjct: 185 VVDGNGETWWQ--NSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCS 244

Query: 218 NVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPS 277
           NV V N+ V+AP++SP T GI   ++ ++ +    I TG D I+++SG           S
Sbjct: 245 NVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------S 304

Query: 278 KNIHIRRVHLQSSSGSSI-AFGSEMSGG-ISNVLVEHVHLYNSSTGIQFRTTKGRGGYIK 337
           +N+ I  +      G SI + G + S   +S V V+   L  +  G++ +T +G  G   
Sbjct: 305 QNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTAS 364

Query: 338 GIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNI-EIAGNFTG 397
            I+  +++M+N+         +        + +A+  V+++  +++ GT+  E A  F  
Sbjct: 365 NIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNC 424

Query: 398 IQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESVIPPPCS 431
            +  P   I L  +  +I    +T   C++ +   +  + P C+
Sbjct: 425 SKNYPCQGIVLDRV--NIKGGKAT---CTNANVVDKGAVLPQCN 431

BLAST of Tan0002172 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.3e-25
Identity = 111/407 (27.27%), Postives = 183/407 (44.96%), Query Frame = 0

Query: 39  HSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTL 98
           +++ + +FGAVGDG T ++ AF  A  +    +  G  Q  VP G        +T  L  
Sbjct: 22  NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG--------MTFMLQP 81

Query: 99  FLEKGATILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDG 158
              +G+       P   +++  L +  +G     K    L    +++ +VI G DG I+G
Sbjct: 82  LKFQGSC---KSTPVFVQMLGKLVAPSKGNWKGDKDQWILFT--DIEGLVIEG-DGEING 141

Query: 159 QGLVWWDWFSSHSLNYSRPHLVEFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV 218
           QG  WW+         SRP  ++F     + +S LT L++P  +IH   C+ V + ++ +
Sbjct: 142 QGSSWWEH------KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRI 201

Query: 219 SAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVH 278
           +AP  SP T GI   +S +V I+ C IATG D IA+ SG           + NIHI  + 
Sbjct: 202 NAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHISGI- 261

Query: 279 LQSSSGSSIAFGSEMSGG----ISNVLVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDV 338
                G  I+ GS    G    + NV V++ +   +  G + +T +G  GY + I  + +
Sbjct: 262 -DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGI 321

Query: 339 EMENISTGFSASGHF-GSHPDDEFDPNALPI-VQDITLQNVRGTN-IEIAGNFTGIQESP 398
            ++N+         + G   D+  D  +  + V  +   N  GT+  E   +F   +  P
Sbjct: 322 TLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVP 381

Query: 399 FTSIYLSNITFSINSSSS---TSWICSDVSGYSESVIPP-PCSDLNT 435
            T I+L ++     SS S       C +V G S   +P   C +L+T
Sbjct: 382 CTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELST 393

BLAST of Tan0002172 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 5.8e-24
Identity = 108/406 (26.60%), Postives = 175/406 (43.10%), Query Frame = 0

Query: 42  SILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLT------SH 101
           ++ ++GAVGDGK  +T AF        +   K  A L VP  K    + NL        H
Sbjct: 60  NVEQYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVN-NLVFRGPCQPH 119

Query: 102 LTLFLEKGATILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGV 161
           L+  ++   TI+   DP+ W                 K  +  +    L D  + G  GV
Sbjct: 120 LSFKVD--GTIVAQPDPARW-----------------KNSKIWLQFAQLTDFNLMG-TGV 179

Query: 162 IDGQGLVWW----DWFSSHSL--NYSRPHLVEFEDSKYVVVSNLTFLNTPAYNIHPVYCS 221
           IDGQG  WW       +  ++  + +RP  ++ + SK V V  LT +N+P +++    C 
Sbjct: 180 IDGQGQQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECE 239

Query: 222 NVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPS 281
            V +  + + AP +SP T GI   +S    IE C I TG D IA+ +G           S
Sbjct: 240 GVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------S 299

Query: 282 KNIHIRRVHLQSSSGSSI-AFGSEMS-GGISNVLVEHVHLYNSSTGIQFRTTKGRGGYIK 341
            NI I+ +      G SI + G + S   +S+V V      ++  G++ +T +G  G   
Sbjct: 300 SNITIKDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLAS 359

Query: 342 GIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNIEIAGNFTGI 401
            I   +VEM N       +  + +      +  +   +Q +T +N+ GT+   A      
Sbjct: 360 YITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMC 419

Query: 402 QES-PFTSIYLSNITFSINSSSSTSWICSDVSGYSESVIPPPCSDL 433
            +S P T I LSN++  + S    S +  +  G+    + P C +L
Sbjct: 420 SDSVPCTGIQLSNVSLKLTSGKPASCVDKNARGFYSGRLIPTCKNL 430

BLAST of Tan0002172 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.7e-23
Identity = 105/406 (25.86%), Postives = 177/406 (43.60%), Query Frame = 0

Query: 42  SILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPG-KWLTGSINLTS----HL 101
           ++ ++GAVGDGK   T AF  A    ++   K  A L VP   K++  ++        H 
Sbjct: 60  NVEKYGAVGDGKHDCTEAFSTA---WQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHF 119

Query: 102 TLFLEKGATILGSQDPSHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVI 161
           T  ++    I   Q+P+ W                 K  R  +    L    + G  GVI
Sbjct: 120 TFKVD--GIIAAYQNPASW-----------------KNNRIWLQFAKLTGFTLMG-KGVI 179

Query: 162 DGQGLVWW----DWFSSHSL--NYSRPHLVEFEDSKYVVVSNLTFLNTPAYNIHPVYCSN 221
           DGQG  WW     W +   +  +  RP  ++F+ S  +++  L  +N+P +++    C  
Sbjct: 180 DGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEG 239

Query: 222 VYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSK 281
           V +  IS++AP +SP T GI   +S +  ++   I TG D +A+ +G           S 
Sbjct: 240 VKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTG-----------SS 299

Query: 282 NIHIRRVHLQSSSGSSI-AFGSEMS-GGISNVLVEHVHLYNSSTGIQFRTTKGRGGYIKG 341
           NI I  +      G SI + G E S   +S V V      ++  G++ +T +G  G    
Sbjct: 300 NIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASH 359

Query: 342 IVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDITLQNVRGTNIEIAGNFTGIQ 401
           I+  +VEM N       +  + +      +  +   +QD+T +N+RGT+   A       
Sbjct: 360 IIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCS 419

Query: 402 ES-PFTSIYLSNITFSINSSSSTSWICSDVSGYSESVIPPPCSDLN 434
           +S P   I LS+I+  + S    S +  + +GY    + P C +L+
Sbjct: 420 DSMPCKDIKLSDISLKLTSGKIASCLNDNANGYFSGHVIPACKNLS 431

BLAST of Tan0002172 vs. NCBI nr
Match: XP_038881575.1 (probable polygalacturonase isoform X4 [Benincasa hispida] >XP_038881581.1 probable polygalacturonase isoform X4 [Benincasa hispida])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 436/456 (95.61%), Postives = 447/456 (98.03%), Query Frame = 0

Query: 1   MRMLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 60
           MRMLVALLLLLQLSNAAK+IGEE+AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF
Sbjct: 1   MRMLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 60

Query: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 120
           QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVNP
Sbjct: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNP 120

Query: 121 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLV 180
           LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHLV
Sbjct: 121 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLV 180

Query: 181 EFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 240
           EFEDS+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI
Sbjct: 181 EFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 240

Query: 241 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 300
           EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV
Sbjct: 241 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 300

Query: 301 LVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 360
           LVEHV L NS  GIQFRT KGRGGYIKGIV+SDVEMENIST FSASGHF SHPDDEFDPN
Sbjct: 301 LVEHVQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDPN 360

Query: 361 ALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGY 420
           ALPIVQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSW+CSDVSG+
Sbjct: 361 ALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSGF 420

Query: 421 SESVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           SESVIPPPCSDL+TPYSISSSAAS L+NSNGKTAVL
Sbjct: 421 SESVIPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 456

BLAST of Tan0002172 vs. NCBI nr
Match: XP_038881588.1 (probable polygalacturonase isoform X5 [Benincasa hispida])

HSP 1 Score: 879.4 bits (2271), Expect = 1.3e-251
Identity = 434/454 (95.59%), Postives = 445/454 (98.02%), Query Frame = 0

Query: 3   MLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 62
           MLVALLLLLQLSNAAK+IGEE+AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60

Query: 63  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLP 122
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVNPLP
Sbjct: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 120

Query: 123 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEF 182
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHLVEF
Sbjct: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 180

Query: 183 EDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 242
           EDS+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG
Sbjct: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240

Query: 243 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 302
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300

Query: 303 EHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNAL 362
           EHV L NS  GIQFRT KGRGGYIKGIV+SDVEMENIST FSASGHF SHPDDEFDPNAL
Sbjct: 301 EHVQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDPNAL 360

Query: 363 PIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSE 422
           PIVQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSW+CSDVSG+SE
Sbjct: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSGFSE 420

Query: 423 SVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           SVIPPPCSDL+TPYSISSSAAS L+NSNGKTAVL
Sbjct: 421 SVIPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 454

BLAST of Tan0002172 vs. NCBI nr
Match: XP_022972277.1 (probable polygalacturonase isoform X2 [Cucurbita maxima])

HSP 1 Score: 878.2 bits (2268), Expect = 3.0e-251
Identity = 431/456 (94.52%), Postives = 446/456 (97.81%), Query Frame = 0

Query: 1   MRMLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 60
           MRMLVALLLLLQLSNAAK+IG+E++GQC+SKP LDPRPHSVSILEFGAVGDGKTLNTIAF
Sbjct: 1   MRMLVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAF 60

Query: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 120
           QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP
Sbjct: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 120

Query: 121 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLV 180
           LPSYGRG+EVPGKRYRSLINGYNL DVVITGDDGVIDGQGLVWWDWF+SHSLNYSRPHLV
Sbjct: 121 LPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLV 180

Query: 181 EFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 240
           EFEDS+YVVVSNL+FLNTPAYNIHPVYCSNVYV+NISVSAPSESPYTVGIVPDSSDHVCI
Sbjct: 181 EFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCI 240

Query: 241 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 300
           EGCNIATGYDAI LKSGWDQYGIAYGRPSKNIHIR VHLQSSSGSSIAFGSEMSGGISNV
Sbjct: 241 EGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNV 300

Query: 301 LVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 360
           LVEHVHLYNSSTGI+FRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN
Sbjct: 301 LVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 360

Query: 361 ALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGY 420
           ALPIVQDITLQNVRGTNI IAGNFTG+QESPFTSIYLSNITFS+NSSSSTSWIC DVSGY
Sbjct: 361 ALPIVQDITLQNVRGTNINIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICLDVSGY 420

Query: 421 SESVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           SESVIPPPCSDL+T YSISS AA+SLLNS GK AVL
Sbjct: 421 SESVIPPPCSDLDTRYSISSLAATSLLNSAGKAAVL 456

BLAST of Tan0002172 vs. NCBI nr
Match: XP_038881565.1 (probable polygalacturonase isoform X3 [Benincasa hispida])

HSP 1 Score: 877.1 bits (2265), Expect = 6.6e-251
Identity = 432/452 (95.58%), Postives = 443/452 (98.01%), Query Frame = 0

Query: 5   VALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 64
           VALLLLLQLSNAAK+IGEE+AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 65  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY 124
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 125 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEFED 184
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 185 SKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 244
           S+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 245 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 304
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 305 VHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 364
           V L NS  GIQFRT KGRGGYIKGIV+SDVEMENIST FSASGHF SHPDDEFDPNALPI
Sbjct: 307 VQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDPNALPI 366

Query: 365 VQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESV 424
           VQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSW+CSDVSG+SESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSGFSESV 426

Query: 425 IPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           IPPPCSDL+TPYSISSSAAS L+NSNGKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 458

BLAST of Tan0002172 vs. NCBI nr
Match: XP_038881556.1 (probable polygalacturonase isoform X2 [Benincasa hispida])

HSP 1 Score: 877.1 bits (2265), Expect = 6.6e-251
Identity = 432/452 (95.58%), Postives = 443/452 (98.01%), Query Frame = 0

Query: 5   VALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 64
           VALLLLLQLSNAAK+IGEE+AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 22  VALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 81

Query: 65  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY 124
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVNPLPSY
Sbjct: 82  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 141

Query: 125 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEFED 184
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHLVEFED
Sbjct: 142 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 201

Query: 185 SKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 244
           S+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 202 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 261

Query: 245 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 304
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 262 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 321

Query: 305 VHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 364
           V L NS  GIQFRT KGRGGYIKGIV+SDVEMENIST FSASGHF SHPDDEFDPNALPI
Sbjct: 322 VQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDPNALPI 381

Query: 365 VQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESV 424
           VQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSW+CSDVSG+SESV
Sbjct: 382 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSGFSESV 441

Query: 425 IPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           IPPPCSDL+TPYSISSSAAS L+NSNGKTAVL
Sbjct: 442 IPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 473

BLAST of Tan0002172 vs. ExPASy TrEMBL
Match: A0A6J1I4D7 (probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470858 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.4e-251
Identity = 431/456 (94.52%), Postives = 446/456 (97.81%), Query Frame = 0

Query: 1   MRMLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 60
           MRMLVALLLLLQLSNAAK+IG+E++GQC+SKP LDPRPHSVSILEFGAVGDGKTLNTIAF
Sbjct: 1   MRMLVALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAF 60

Query: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 120
           QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP
Sbjct: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 120

Query: 121 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLV 180
           LPSYGRG+EVPGKRYRSLINGYNL DVVITGDDGVIDGQGLVWWDWF+SHSLNYSRPHLV
Sbjct: 121 LPSYGRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLV 180

Query: 181 EFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 240
           EFEDS+YVVVSNL+FLNTPAYNIHPVYCSNVYV+NISVSAPSESPYTVGIVPDSSDHVCI
Sbjct: 181 EFEDSEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCI 240

Query: 241 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 300
           EGCNIATGYDAI LKSGWDQYGIAYGRPSKNIHIR VHLQSSSGSSIAFGSEMSGGISNV
Sbjct: 241 EGCNIATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNV 300

Query: 301 LVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 360
           LVEHVHLYNSSTGI+FRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN
Sbjct: 301 LVEHVHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 360

Query: 361 ALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGY 420
           ALPIVQDITLQNVRGTNI IAGNFTG+QESPFTSIYLSNITFS+NSSSSTSWIC DVSGY
Sbjct: 361 ALPIVQDITLQNVRGTNINIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICLDVSGY 420

Query: 421 SESVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           SESVIPPPCSDL+T YSISS AA+SLLNS GK AVL
Sbjct: 421 SESVIPPPCSDLDTRYSISSLAATSLLNSAGKAAVL 456

BLAST of Tan0002172 vs. ExPASy TrEMBL
Match: A0A0A0LAN4 (Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165090 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.2e-250
Identity = 434/457 (94.97%), Postives = 445/457 (97.37%), Query Frame = 0

Query: 1   MRM-LVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRM LVALLL LQLSNAAK+IGEE+ GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRMLLVALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDS+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDP 360
           VLVEHV L NS  GIQ RTTKGRGGYIKGIV+SDVEMENIST FSASGHFGSHPDDE+DP
Sbjct: 301 VLVEHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360

Query: 361 NALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSWICSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420

Query: 421 YSESVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           +SESVIPPPCSDL+TPYSISSSAAS L+NS GKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of Tan0002172 vs. ExPASy TrEMBL
Match: A0A1S3AXL3 (probable polygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.1e-250
Identity = 433/457 (94.75%), Postives = 446/457 (97.59%), Query Frame = 0

Query: 1   MRM-LVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MRM LVALLL LQLSNAA++IGEE+AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 1   MRMLLVALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVN
Sbjct: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVN 120

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHL
Sbjct: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 180

Query: 181 VEFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDS+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVC
Sbjct: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVC 240

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300

Query: 301 VLVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDP 360
           VLVEHV L NS  GI+FRTTKGRGGYIKGIV+SDVEMENIST FSASGHFGSHPDDE+DP
Sbjct: 301 VLVEHVQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDP 360

Query: 361 NALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSWICSDVSG
Sbjct: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420

Query: 421 YSESVIPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           +SESVIPPPCSDL+TPYSISSSAAS L+NS GKTAVL
Sbjct: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of Tan0002172 vs. ExPASy TrEMBL
Match: A0A1S3AX49 (probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.6e-250
Identity = 429/452 (94.91%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 5   VALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 64
           VALLL LQLSNAA++IGEE+AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 65  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY 124
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGA ILGSQDPSHWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 125 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEFED 184
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWW+WFSSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 185 SKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 244
           S+YVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 245 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 304
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 305 VHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 364
           V L NS  GI+FRTTKGRGGYIKGIV+SDVEMENIST FSASGHFGSHPDDE+DPNALPI
Sbjct: 307 VQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 366

Query: 365 VQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESV 424
           VQDITLQNVRGTNI+IAGNF+GIQESPFTSIYLSNITFSINSSSSTSWICSDVSG+SESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 426

Query: 425 IPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           IPPPCSDL+TPYSISSSAAS L+NS GKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

BLAST of Tan0002172 vs. ExPASy TrEMBL
Match: A0A6J1I844 (probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470858 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 7.9e-250
Identity = 427/452 (94.47%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 5   VALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 64
           VALLLLLQLSNAAK+IG+E++GQC+SKP LDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 8   VALLLLLQLSNAAKIIGDELSGQCNSKPILDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 67

Query: 65  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY 124
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY
Sbjct: 68  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSY 127

Query: 125 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEFED 184
           GRG+EVPGKRYRSLINGYNL DVVITGDDGVIDGQGLVWWDWF+SHSLNYSRPHLVEFED
Sbjct: 128 GRGVEVPGKRYRSLINGYNLHDVVITGDDGVIDGQGLVWWDWFTSHSLNYSRPHLVEFED 187

Query: 185 SKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 244
           S+YVVVSNL+FLNTPAYNIHPVYCSNVYV+NISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 188 SEYVVVSNLSFLNTPAYNIHPVYCSNVYVFNISVSAPSESPYTVGIVPDSSDHVCIEGCN 247

Query: 245 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 304
           IATGYDAI LKSGWDQYGIAYGRPSKNIHIR VHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 248 IATGYDAIVLKSGWDQYGIAYGRPSKNIHIRNVHLQSSSGSSIAFGSEMSGGISNVLVEH 307

Query: 305 VHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 364
           VHLYNSSTGI+FRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI
Sbjct: 308 VHLYNSSTGIEFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPI 367

Query: 365 VQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESV 424
           VQDITLQNVRGTNI IAGNFTG+QESPFTSIYLSNITFS+NSSSSTSWIC DVSGYSESV
Sbjct: 368 VQDITLQNVRGTNINIAGNFTGLQESPFTSIYLSNITFSMNSSSSTSWICLDVSGYSESV 427

Query: 425 IPPPCSDLNTPYSISSSAASSLLNSNGKTAVL 457
           IPPPCSDL+T YSISS AA+SLLNS GK AVL
Sbjct: 428 IPPPCSDLDTRYSISSLAATSLLNSAGKAAVL 459

BLAST of Tan0002172 vs. TAIR 10
Match: AT3G06770.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 576.6 bits (1485), Expect = 1.7e-164
Identity = 280/434 (64.52%), Postives = 347/434 (79.95%), Query Frame = 0

Query: 3   MLVALLLLLQLSNAAKVIGE-EVAGQ-CD--SKPTLDPRPHSVSILEFGAVGDGKTLNTI 62
           M V  +L+L L++   +      AG+ CD   + +L  RPHSVSI +FGAVGDGKTLNT+
Sbjct: 1   MSVVTILILALTSVIPIYANVHGAGKICDELGRRSLSTRPHSVSITDFGAVGDGKTLNTL 60

Query: 63  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELV 122
           AFQNA+FYL SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GA I+ SQDPSHWE+V
Sbjct: 61  AFQNAVFYLMSFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVV 120

Query: 123 NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPH 182
           +PLPSYGRGI++PGKRY+SLING  L DVV+TGD+G IDGQGLVWWD F+SHSL Y+RPH
Sbjct: 121 DPLPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPH 180

Query: 183 LVEFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHV 242
           L+EF  S+ V+VSNLTFLN PAY+I+ +Y S+VY++ I   +  +SPYT+GIVPDSSD+V
Sbjct: 181 LIEFLSSENVIVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYV 240

Query: 243 CIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS 302
           CI+   I  GYDAI+LKSGWD+YGIAY RP++N+HIR V+L+ +SGSSI+FGSEMSGGIS
Sbjct: 241 CIQNSTINVGYDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGIS 300

Query: 303 NVLVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFD 362
           +V+V++ H++ S TGI FRTTKGRGGYIK I IS+++M  I T   A+G FGSHPDD++D
Sbjct: 301 DVVVDNAHIHYSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYD 360

Query: 363 PNALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVS 422
            NALP+V  I L N+ G NI IAG   GI+ESPF+S+ LSN++ S++S SS SW CS V 
Sbjct: 361 VNALPLVSHIRLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVY 420

Query: 423 GYSESVIPPPCSDL 433
           G SESVIP PC +L
Sbjct: 421 GSSESVIPEPCPEL 434

BLAST of Tan0002172 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 570.9 bits (1470), Expect = 9.5e-163
Identity = 278/454 (61.23%), Postives = 348/454 (76.65%), Query Frame = 0

Query: 1   MRMLVALLLLLQLSNAAKVIGEEVAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 60
           M+M VAL+ LL  +    + GE     C     LDPRPHSVSILEFGAVGDGKTLNTIAF
Sbjct: 1   MKMPVALVWLLAFT-ILLISGEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTIAF 60

Query: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 120
           QNA+FYLKSFADKGGAQLYVPPGKWLTGS NLTSHLTLFLEKGATIL S DPSHW++V+P
Sbjct: 61  QNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVVSP 120

Query: 121 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLV 180
           LPSYGRGIE+PGKRYRSLING NL DVVITG++G  DGQG  WW+W  S SLNYSRPH++
Sbjct: 121 LPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPHII 180

Query: 181 EFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 240
           EF  SK++++SNLTFLN PA NIHPVYCS +++  + +    +SP+ +G+ PDSSD+VCI
Sbjct: 181 EFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNVCI 240

Query: 241 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 300
           E   I  G+DA++LKSGWDQYGI YGRP+  +HIR + L+S +G+ I+FGSEMSGG+S+V
Sbjct: 241 EDSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSDV 300

Query: 301 LVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 360
            VE +++++S  G+ FRTT+GRGGYI+ I ISDV++ ++ T   A+GH GSHPDD+FD +
Sbjct: 301 TVERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDRD 360

Query: 361 ALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGY 420
           ALP+V  I ++N  G +I +AGN TGI ESPFTSI L++I   + + S  SWICS+VSG+
Sbjct: 361 ALPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADI--HLQTRSEESWICSNVSGF 420

Query: 421 SESVIPPPCSDLNTPYSISSSAASSLLNSNGKTA 455
           S+ V P PC +L +  S SS  A   +     TA
Sbjct: 421 SDDVSPEPCQELMS--SPSSCFAGGSIYEGDATA 449

BLAST of Tan0002172 vs. TAIR 10
Match: AT5G49215.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 560.8 bits (1444), Expect = 9.8e-160
Identity = 277/436 (63.53%), Postives = 336/436 (77.06%), Query Frame = 0

Query: 3   MLVALLLLLQLSNAAKVIGEE--VAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 62
           M VAL+LLL LS+   + G    V   CD       RPHSV I +FGAVGDGKTLNT+AF
Sbjct: 1   MPVALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAF 60

Query: 63  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNP 122
           QNA+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK A IL SQDPSHW++ + 
Sbjct: 61  QNAVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDA 120

Query: 123 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLV 182
           LPSYGRGI++PGKRY SLING  L DVV+TGD+G IDGQGLVWWD F+SHSL YSRPHLV
Sbjct: 121 LPSYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLV 180

Query: 183 EFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 242
           EF  ++ V+VSNLTFLN PAY IH VYC N+Y++ ++ +   ESPYT+GIVPDSS++VCI
Sbjct: 181 EFVSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCI 240

Query: 243 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 302
           +  +I  GYDAI+LKSG D+YG++Y RP+ N+ IR V+L+++SGSSI+FGSEMSGGIS+V
Sbjct: 241 QESSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDV 300

Query: 303 LVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPN 362
            V   H++NS +GI FRTT GRGGYIK I IS++ M N+ T F A+G FG+HPD  FD N
Sbjct: 301 EVSDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDEN 360

Query: 363 ALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSS--TSWICSDVS 422
           A P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ SI +S+S   SW CS V 
Sbjct: 361 AYPLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVD 420

Query: 423 GYSESVIPPPCSDLNT 435
           G SE V+P PC +L +
Sbjct: 421 GSSEFVVPEPCLELKS 436

BLAST of Tan0002172 vs. TAIR 10
Match: AT5G49215.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 560.5 bits (1443), Expect = 1.3e-159
Identity = 277/438 (63.24%), Postives = 337/438 (76.94%), Query Frame = 0

Query: 1   MRMLVALLLLLQLSNAAKVIGEE--VAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTI 60
           M + VAL+LLL LS+   + G    V   CD       RPHSV I +FGAVGDGKTLNT+
Sbjct: 1   MPVSVALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTL 60

Query: 61  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELV 120
           AFQNA+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK A IL SQDPSHW++ 
Sbjct: 61  AFQNAVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVT 120

Query: 121 NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPH 180
           + LPSYGRGI++PGKRY SLING  L DVV+TGD+G IDGQGLVWWD F+SHSL YSRPH
Sbjct: 121 DALPSYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPH 180

Query: 181 LVEFEDSKYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHV 240
           LVEF  ++ V+VSNLTFLN PAY IH VYC N+Y++ ++ +   ESPYT+GIVPDSS++V
Sbjct: 181 LVEFVSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENV 240

Query: 241 CIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS 300
           CI+  +I  GYDAI+LKSG D+YG++Y RP+ N+ IR V+L+++SGSSI+FGSEMSGGIS
Sbjct: 241 CIQESSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGIS 300

Query: 301 NVLVEHVHLYNSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFD 360
           +V V   H++NS +GI FRTT GRGGYIK I IS++ M N+ T F A+G FG+HPD  FD
Sbjct: 301 DVEVSDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFD 360

Query: 361 PNALPIVQDITLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSS--TSWICSD 420
            NA P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ SI +S+S   SW CS 
Sbjct: 361 ENAYPLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSY 420

Query: 421 VSGYSESVIPPPCSDLNT 435
           V G SE V+P PC +L +
Sbjct: 421 VDGSSEFVVPEPCLELKS 438

BLAST of Tan0002172 vs. TAIR 10
Match: AT3G06770.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 515.8 bits (1327), Expect = 3.6e-146
Identity = 243/364 (66.76%), Postives = 299/364 (82.14%), Query Frame = 0

Query: 69  SFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGATILGSQDPSHWELVNPLPSYGRGI 128
           SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GA I+ SQDPSHWE+V+PLPSYGRGI
Sbjct: 2   SFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVVDPLPSYGRGI 61

Query: 129 EVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWDWFSSHSLNYSRPHLVEFEDSKYV 188
           ++PGKRY+SLING  L DVV+TGD+G IDGQGLVWWD F+SHSL Y+RPHL+EF  S+ V
Sbjct: 62  DLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPHLIEFLSSENV 121

Query: 189 VVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATG 248
           +VSNLTFLN PAY+I+ +Y S+VY++ I   +  +SPYT+GIVPDSSD+VCI+   I  G
Sbjct: 122 IVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYVCIQNSTINVG 181

Query: 249 YDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEHVHLY 308
           YDAI+LKSGWD+YGIAY RP++N+HIR V+L+ +SGSSI+FGSEMSGGIS+V+V++ H++
Sbjct: 182 YDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGISDVVVDNAHIH 241

Query: 309 NSSTGIQFRTTKGRGGYIKGIVISDVEMENISTGFSASGHFGSHPDDEFDPNALPIVQDI 368
            S TGI FRTTKGRGGYIK I IS+++M  I T   A+G FGSHPDD++D NALP+V  I
Sbjct: 242 YSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYDVNALPLVSHI 301

Query: 369 TLQNVRGTNIEIAGNFTGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGYSESVIPPP 428
            L N+ G NI IAG   GI+ESPF+S+ LSN++ S++S SS SW CS V G SESVIP P
Sbjct: 302 RLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVYGSSESVIPEP 361

Query: 429 CSDL 433
           C +L
Sbjct: 362 CPEL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL32.0e-11247.10Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q8RY292.1e-2627.23Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Q9LW075.3e-2527.27Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q7M1E75.8e-2426.60Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P432121.7e-2325.86Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038881575.11.2e-25295.61probable polygalacturonase isoform X4 [Benincasa hispida] >XP_038881581.1 probab... [more]
XP_038881588.11.3e-25195.59probable polygalacturonase isoform X5 [Benincasa hispida][more]
XP_022972277.13.0e-25194.52probable polygalacturonase isoform X2 [Cucurbita maxima][more]
XP_038881565.16.6e-25195.58probable polygalacturonase isoform X3 [Benincasa hispida][more]
XP_038881556.16.6e-25195.58probable polygalacturonase isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1I4D71.4e-25194.52probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147085... [more]
A0A0A0LAN41.2e-25094.97Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G16... [more]
A0A1S3AXL32.1e-25094.75probable polygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483871 PE... [more]
A0A1S3AX494.6e-25094.91probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE... [more]
A0A6J1I8447.9e-25094.47probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147085... [more]
Match NameE-valueIdentityDescription
AT3G06770.21.7e-16464.52Pectin lyase-like superfamily protein [more]
AT3G16850.19.5e-16361.23Pectin lyase-like superfamily protein [more]
AT5G49215.29.8e-16063.53Pectin lyase-like superfamily protein [more]
AT5G49215.11.3e-15963.24Pectin lyase-like superfamily protein [more]
AT3G06770.33.6e-14666.76Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 235..256
e-value: 930.0
score: 7.4
coord: 185..207
e-value: 1600.0
score: 5.8
coord: 297..318
e-value: 300.0
score: 10.2
coord: 360..382
e-value: 3900.0
score: 3.0
coord: 269..291
e-value: 780.0
score: 8.0
coord: 326..347
e-value: 2000.0
score: 5.1
coord: 208..234
e-value: 5100.0
score: 2.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 22..421
e-value: 8.4E-113
score: 379.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 132..418
e-value: 1.9E-34
score: 119.2
IPR024535Pectate lyase superfamily proteinPFAMPF12708Pectate_lyase_3coord: 41..98
e-value: 1.2E-7
score: 31.8
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 4..446
NoneNo IPR availablePANTHERPTHR31339:SF15PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 4..446
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 36..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002172.1Tan0002172.1mRNA
Tan0002172.2Tan0002172.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity