Tan0002087 (gene) Snake gourd v1

BLAST of Tan0002087 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.5e-138
Identity = 249/542 (45.94%), Postives = 352/542 (64.94%), Query Frame = 0

Query: 1   MNFDWSYFSFICLLLVSYGRWG--FGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSY 60
           MN     F F+C ++  +  +       N  E+FL C S Y  NN +N   I+T     Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLY 60

Query: 61  ISVLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVS 120
           +SVLN  I+NLRF+  TTP P  I+TP+  S +QA+++C K   LQIRTRSGGHD EG+S
Sbjct: 61  MSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLS 120

Query: 121 YVSKVPFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVG 180
           Y+S+VPF ++DL  ++++ +D    T WVE+GAT+GE+YY I E +    FP    PTVG
Sbjct: 121 YISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVG 180

Query: 181 VGGFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGI 240
           VGG  SGGG+G ++R YGLAADN++DA++V+ +GK+LDR SMGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 241 VLAWKLKLVPVPPIVTSFAVYTSWD-QNAAKLIHRWQYVAPEVDENLFINARVTASNSSE 300
           + AWK+KLV VP   T F+V  + +     KL ++WQ +A + D++L +       N ++
Sbjct: 241 IAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITD 300

Query: 301 GGGR---IMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVS 360
             G+    +   F S++LG  D L+ LM K+FPELG+KK DC E+SW+++  F  SG V+
Sbjct: 301 NHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIF-YSGVVN 360

Query: 361 KESLD---EVLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPY 420
             + +   E+LL+R+        IK DYV + I ET +  I E+   +E+     VL PY
Sbjct: 361 YNTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPY 420

Query: 421 GGKMNEISETETPSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRA 480
           GG M+EISE+  P PHRAG   ++ Y  TW++ + + +H  W + +Y++ TP+VS++PR 
Sbjct: 421 GGIMDEISESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRL 480

Query: 481 AYVNYRDLDMGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSI 534
           AY+NYRDLD+G+ N   E   +Y +A +WG +YFG NF RL+ VKTK DP NFFR+EQSI
Sbjct: 481 AYLNYRDLDLGKTN--PESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSI 539

BLAST of Tan0002087 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.4e-138
Identity = 249/514 (48.44%), Postives = 345/514 (67.12%), Query Frame = 0

Query: 28  NLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPTE 87
           N E+FL C+S     N+ +S +IHT    SY S+LN  I+N RF  + TP P +IITP +
Sbjct: 33  NHENFLKCLS--HRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQ 92

Query: 88  ASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKV-PFVLIDLIKLNSITIDTKDNTTW 147
           A+ VQ+ + C + H + IRTRSGGHD+EG+SY++K  PFV+IDL  L SIT+D  + T W
Sbjct: 93  ATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGW 152

Query: 148 VESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAY 207
           V+SGAT+GELYY+IG+ S+ L FPA + PTVG+GG   GGG+G ++RKYGL+ADNV+DA+
Sbjct: 153 VQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAH 212

Query: 208 IVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWDQNA 267
           IVDANG  LDR  MGED FWAIRGGGG SF +VL+WK++L+ VP +VT F V  + ++ A
Sbjct: 213 IVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEA 272

Query: 268 AKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRASFLSLYLGKADELLSLMEKTF 327
             +I++WQY+A +V  +LFI A +            + ASF  LYLG   +LL+LM+  F
Sbjct: 273 VSIINKWQYIADKVPNDLFIRAMLQKETE-------VYASFPGLYLGPVSDLLALMKDKF 332

Query: 328 PELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRTPLNSGRHKIKSDYVTEPISE 387
           PELGL+  +C EMSW+ES+ +    F+  ES++  +L +    S   K K D++ EPI +
Sbjct: 333 PELGLEIGNCREMSWIESVLW----FIKGESME--ILAKRKRTSRSFKGKDDFIEEPIPK 392

Query: 388 TVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYVTWQRPD- 447
           T ++ +W RF+  E    +++L P+GGKM+EI++ E P PHR G   +I Y   W   + 
Sbjct: 393 TAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEED 452

Query: 448 ----ADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASVWG 507
                  ++  W + +Y++MTP+VSKSPR AYVN+RD+D+G     +  TK YEEA VWG
Sbjct: 453 KNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTK-YEEAKVWG 512

Query: 508 YQYFGNNFERLIDVKTKVDPFNFFRHEQSIPPAK 536
            +YF NNF+RL+ VKT VDP +FF  EQSIP  K
Sbjct: 513 VKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIMK 530

BLAST of Tan0002087 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.4e-138
Identity = 244/513 (47.56%), Postives = 339/513 (66.08%), Query Frame = 0

Query: 27  NNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPT 86
           N  E+FL C S +  NN +N  L++T     Y+S+LN  I+NLRF   TTP P  I+TP+
Sbjct: 29  NPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPS 88

Query: 87  EASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPFVLIDLIKLNSITIDTKDNTTW 146
             S +QA ++C K   LQIRTRSGGHD EG+SY+S+VPFV++DL  ++SI ID    T W
Sbjct: 89  NNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAW 148

Query: 147 VESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAY 206
           VE+GAT+GE+YY I EK+  L FP    PTVGVGG  SGGG+G ++R YGLAADN++DA+
Sbjct: 149 VEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAH 208

Query: 207 IVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWD-QN 266
           +V+ +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T F+V  + +   
Sbjct: 209 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHG 268

Query: 267 AAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGR---IMRASFLSLYLGKADELLSLM 326
             KL ++WQ +A + D++L +       N ++  G+    +   F S++ G  D L+ LM
Sbjct: 269 LVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLM 328

Query: 327 EKTFPELGLKKEDCLEMSWVESMAFSAS--GFVSKESLDEVLLNRTPLNSGRHKIKSDYV 386
            K+FPELG+KK DC E SW+++  F +    F +     E+LL+R+        IK DYV
Sbjct: 329 NKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYV 388

Query: 387 TEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYVT 446
            +PI ET +  I E+   +++     VL PYGG M EISE+  P PHRAG   ++ Y  +
Sbjct: 389 KKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTAS 448

Query: 447 WQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASVW 506
           W++ + + +H  W + +Y++ TP+VS++PR AY+NYRDLD+G+ N       +Y +A +W
Sbjct: 449 WEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHAS--PNNYTQARIW 508

Query: 507 GYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP 534
           G +YFG NF RL+ VKTKVDP NFFR+EQSIPP
Sbjct: 509 GEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPP 539

BLAST of Tan0002087 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.5e-138
Identity = 241/514 (46.89%), Postives = 344/514 (66.93%), Query Frame = 0

Query: 27  NNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPT 86
           N  E+FL C+S Y   N +N+ L++T     Y+S+LN  ++NLRF+  TTP P  I TP 
Sbjct: 29  NPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPL 88

Query: 87  EASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPFVLIDLIKLNSITIDTKDNTTW 146
             S +Q  ++C K   LQIRTRSGGHD EG+SY+S+VPFV++DL  ++S+ ID    T W
Sbjct: 89  NVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAW 148

Query: 147 VESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAY 206
           VESGAT+GE+YY I E +  L FPA   PTVG GG  SGGG+G ++R YGLAADN++DA+
Sbjct: 149 VESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAH 208

Query: 207 IVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWD-QN 266
           +V+ +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK++LV VP + T F+V  + +   
Sbjct: 209 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHE 268

Query: 267 AAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGR---IMRASFLSLYLGKADELLSLM 326
             KL+++WQ +A   ++ L +       N ++  G+    + + F S++ G  D L+ LM
Sbjct: 269 LVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLM 328

Query: 327 EKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLD---EVLLNRTPLNSGRHKIKSDY 386
            K+FPELG+KK DC ++SW++++ F  SG V+  + +   E+LL+R+        IK DY
Sbjct: 329 NKSFPELGIKKTDCKQLSWIDTIIF-YSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDY 388

Query: 387 VTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYV 446
           V +PI ET +  I E+   +++     V  PYGG M+EISE+  P PHRAG   +I Y  
Sbjct: 389 VKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIA 448

Query: 447 TWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASV 506
           +W++ + + +H  W + +Y++ TP+VS++PR AY+NYRDLD+G+ N   E   +Y +A +
Sbjct: 449 SWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTN--FESPNNYTQARI 508

Query: 507 WGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP 534
           WG +YFG NF RL+ VKTKVDP NFFR+EQSIPP
Sbjct: 509 WGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPP 539

BLAST of Tan0002087 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.3e-137
Identity = 242/514 (47.08%), Postives = 343/514 (66.73%), Query Frame = 0

Query: 27  NNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPT 86
           N  E+FL C S Y   N +N+ L++T     Y+S+LN  I+NLRF+  TTP P  IITP 
Sbjct: 29  NPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPL 88

Query: 87  EASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPFVLIDLIKLNSITIDTKDNTTW 146
             S +Q  ++C K   LQIRTRSGGHD EG+SY+S+VPFV++DL  ++S+ ID    T W
Sbjct: 89  NVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAW 148

Query: 147 VESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAY 206
           VE+GAT+GE+YY I E +  L FPA   PTVG GG  SGGG+G ++R YGLAADN++DA+
Sbjct: 149 VEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAH 208

Query: 207 IVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWD-QN 266
           +V+ +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK++LV VP + T F+V  + +   
Sbjct: 209 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHE 268

Query: 267 AAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGR---IMRASFLSLYLGKADELLSLM 326
             KL+++WQ +A   ++ L +       N ++  G+    + + F S++ G  D L+ LM
Sbjct: 269 LVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLM 328

Query: 327 EKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESL---DEVLLNRTPLNSGRHKIKSDY 386
            K+FPELG+KK DC ++SW++++ F  SG V+  +     E+LL+R+        IK DY
Sbjct: 329 NKSFPELGIKKTDCKQLSWIDTIIF-YSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDY 388

Query: 387 VTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYV 446
           V +PI ET +  I E+   +++     V  PYGG M+EISE+  P PHRAG   +I Y  
Sbjct: 389 VKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIA 448

Query: 447 TWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASV 506
           +W++ + + +H  W + +Y++ TP+VS++PR AY+NYRDLD+G+ N   E   +Y +A +
Sbjct: 449 SWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTN--FESPNNYTQARI 508

Query: 507 WGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP 534
           WG +YFG NF RL+ VKTKVDP NFFR+EQSIPP
Sbjct: 509 WGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPP 539

BLAST of Tan0002087 vs. NCBI nr
Match: XP_038895067.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 844.3 bits (2180), Expect = 5.9e-241
Identity = 420/531 (79.10%), Postives = 461/531 (86.82%), Query Frame = 0

Query: 7   YFSFICLLLVS---YGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLN 66
           Y+ FI LLL S    G WG+    N+EDF+HCI  +S+NNDSNSTLIHTPSSSSY  VLN
Sbjct: 8   YYGFIFLLLFSLATLGCWGY----NMEDFVHCIYLHSNNNDSNSTLIHTPSSSSYSYVLN 67

Query: 67  FRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKV 126
           F IRNLRFS   TP P AIITP+ ASQVQAAVICCKSH  QIRTRSGGHDFEG SYV+ V
Sbjct: 68  FSIRNLRFSEPETPKPVAIITPSHASQVQAAVICCKSHGFQIRTRSGGHDFEGSSYVANV 127

Query: 127 PFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFL 186
           PFVLIDLI LNSITIDT+D + WV+SGATVGELYY+IGEKSR LGFPA   PT+G+GGFL
Sbjct: 128 PFVLIDLINLNSITIDTEDESAWVQSGATVGELYYRIGEKSRTLGFPAGFVPTIGIGGFL 187

Query: 187 SGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWK 246
           SGGGFGMMVRKYGLAADNVVDAY+VDANG + DR SMGEDLFWAIRGGGGGSFGIVLAWK
Sbjct: 188 SGGGFGMMVRKYGLAADNVVDAYVVDANGSVFDRYSMGEDLFWAIRGGGGGSFGIVLAWK 247

Query: 247 LKLVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIM 306
           LKLVPVPP VTSF++  +WDQNAA LIHRWQYVAPEVD++LFI+A VTA NSS+G GRIM
Sbjct: 248 LKLVPVPPTVTSFSLSKTWDQNAADLIHRWQYVAPEVDQDLFISAWVTAFNSSDGSGRIM 307

Query: 307 RASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLL 366
            ASF SLYLGKA ELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVS +SL E+LL
Sbjct: 308 EASFFSLYLGKATELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSAKSL-ELLL 367

Query: 367 NRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETET 426
           NR+PL++GR KIKSDY TEPISET LEG+WERFK +ELE +Q +LIP+GGKMNEISE+ET
Sbjct: 368 NRSPLDNGRQKIKSDYPTEPISETALEGMWERFKNEELEKVQFILIPFGGKMNEISESET 427

Query: 427 PSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGR 486
           PS HRAGYPI IGYYV WQ PDAD RH EW +ELY+YMTPFVSKSPRAAY NYRDLD+G 
Sbjct: 428 PSAHRAGYPIHIGYYVKWQHPDADSRHLEWTRELYNYMTPFVSKSPRAAYANYRDLDIGT 487

Query: 487 NNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPPA 535
           NN +D I  SYEEASVWG+QYFGNNFERL++VK KVD FNFFRHEQSIPPA
Sbjct: 488 NN-EDRILTSYEEASVWGHQYFGNNFERLMEVKAKVDQFNFFRHEQSIPPA 532

BLAST of Tan0002087 vs. NCBI nr
Match: XP_038894011.1 (berberine bridge enzyme-like 18 isoform X2 [Benincasa hispida])

HSP 1 Score: 840.5 bits (2170), Expect = 8.6e-240
Identity = 416/545 (76.33%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 1   MNFDW-SYFSFI-CLLLVSYGRWGFGGDNNLEDFLHCISGYSS-NNDSNSTLIHTPSSSS 60
           M + W SY+SFI  LLL+S    G  GDN  EDF+HCIS +SS NNDSN+TLIHTPSSSS
Sbjct: 1   MKYFWSSYYSFISLLLLLSLATLGCWGDNG-EDFVHCISLHSSNNNDSNTTLIHTPSSSS 60

Query: 61  YISVLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGV 120
           Y  VLNF IRNLRFS   TP P AIITP+ AS VQAAVICCKSH LQIRTRSGGHDFEG 
Sbjct: 61  YSYVLNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGR 120

Query: 121 SYVSKVPFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTV 180
           SYV+ VPFVLIDLI LNSITIDT+D + WV+SGAT+G+LYY+I EKS+ LGFPA   PT+
Sbjct: 121 SYVANVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTI 180

Query: 181 GVGGFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFG 240
           GVGG+LSGGGFGMMVRKYGLA DNVVDAY+VDANG++ DR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGYLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVLAWKLKLVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSE 300
           IVLAWKLKLVPVPPIVTSFA++ +WDQNAA LIHRWQYVAPEVDE+LFI A VTASNSS+
Sbjct: 241 IVLAWKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTASNSSK 300

Query: 301 GGGRIMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKES 360
            GGRIM ASF SL+LGKAD+LLSLM+KTFPELGLKKEDCLEMSW+ESMAF ASGFVS  +
Sbjct: 301 EGGRIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAFMASGFVSANN 360

Query: 361 LDEVLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNE 420
           + ++LL+RTPL++ R+K KSDY T+PIS+T LEG+WERFKI+ELET+QL+LIP+GGKMNE
Sbjct: 361 V-KLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFKIEELETVQLMLIPFGGKMNE 420

Query: 421 ISETETPSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYR 480
           ISETETP  +RAGYPI+IGYYVTWQ PDADLRH +W QEL+DYMTPFVSKSPR AY+NY+
Sbjct: 421 ISETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINYK 480

Query: 481 DLDMGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPPAKIT 540
           DLD+G  N +D I  SYEEA VWG+ YFGNNFERL++VK KVDPFNFFRHEQSIPPAK+ 
Sbjct: 481 DLDIG-TNKEDGIPTSYEEAGVWGHPYFGNNFERLVEVKMKVDPFNFFRHEQSIPPAKMA 540

Query: 541 SCSHI 543
           SC  +
Sbjct: 541 SCPDV 542

BLAST of Tan0002087 vs. NCBI nr
Match: KAG6583541.1 (hypothetical protein SDJN03_19473, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 836.6 bits (2160), Expect = 1.2e-238
Identity = 413/547 (75.50%), Postives = 470/547 (85.92%), Query Frame = 0

Query: 1   MNFDWSYFSFICLLLVSYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYIS 60
           M   WS FSFI LLL+S+  WG G D NL+DFLHCIS +SSN+ SNSTLIHTPSSSSY  
Sbjct: 1   MKIYWSNFSFISLLLLSFASWGCGSD-NLQDFLHCISLHSSNHHSNSTLIHTPSSSSYPY 60

Query: 61  VLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYV 120
           VLNF IRNLRF+   TP P AI+TP + S+VQA VICCK+H LQIRTRSGGHDFEG SYV
Sbjct: 61  VLNFSIRNLRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYV 120

Query: 121 SKVPFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVG 180
           S VPFVLIDLI LNSITID  + T W +SGATVGELYYKIGEKSR L FPA    ++G+G
Sbjct: 121 SNVPFVLIDLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLG 180

Query: 181 GFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVL 240
           GF SGGG GM+VRKYGLA DNVVDAY+VDANGKLLDR+SMGEDLFWAIRGGGGGSFGIVL
Sbjct: 181 GFFSGGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVL 240

Query: 241 AWKLKLVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEGGG 300
           AWKLKLV VPP VTSFAV+ SWD NAAKLIHRWQYVAP+VDE+LFI ARVTASNSS+ GG
Sbjct: 241 AWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITARVTASNSSKEGG 300

Query: 301 RIMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLDE 360
           R+M+ASF SL+LGKA EL+SLM+K FPEL LKKEDCLE+SWVE+MAFSAS +VS E+L E
Sbjct: 301 RVMKASFFSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAFSASEYVSPENL-E 360

Query: 361 VLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISE 420
           +LL+RTPL +GR+K+KSDY T+PISET L GIWERFKI+ELET+QL+LIP+GGKMNEISE
Sbjct: 361 LLLDRTPLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISE 420

Query: 421 TETPSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLD 480
           T+TP+PHRA +PI IGYYVTW RPDAD RH EW +EL+DYMTPFVS+SPRAAY+NY+DLD
Sbjct: 421 TDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLD 480

Query: 481 MGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP-----AK 540
           +GRNN +D I  SY+EASVWGY+YFG NFERL++VKTKVDPFNFFRHEQSIPP     AK
Sbjct: 481 IGRNN-EDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAGGIQAK 540

Query: 541 ITSCSHI 543
           + SC  +
Sbjct: 541 MASCPDV 544

BLAST of Tan0002087 vs. NCBI nr
Match: XP_038894010.1 (berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 835.9 bits (2158), Expect = 2.1e-238
Identity = 416/546 (76.19%), Postives = 474/546 (86.81%), Query Frame = 0

Query: 1   MNFDW-SYFSFI-CLLLVSYGRWGFGGDNNLEDFLHCISGYSS-NNDSNSTLIHTPSSSS 60
           M + W SY+SFI  LLL+S    G  GDN  EDF+HCIS +SS NNDSN+TLIHTPSSSS
Sbjct: 1   MKYFWSSYYSFISLLLLLSLATLGCWGDNG-EDFVHCISLHSSNNNDSNTTLIHTPSSSS 60

Query: 61  YISVLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGV 120
           Y  VLNF IRNLRFS   TP P AIITP+ AS VQAAVICCKSH LQIRTRSGGHDFEG 
Sbjct: 61  YSYVLNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEGR 120

Query: 121 SYVSKVPFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTV 180
           SYV+ VPFVLIDLI LNSITIDT+D + WV+SGAT+G+LYY+I EKS+ LGFPA   PT+
Sbjct: 121 SYVANVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPTI 180

Query: 181 GVGGFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFG 240
           GVGG+LSGGGFGMMVRKYGLA DNVVDAY+VDANG++ DR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGYLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVLAWKLKLVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVT-ASNSS 300
           IVLAWKLKLVPVPPIVTSFA++ +WDQNAA LIHRWQYVAPEVDE+LFI A VT ASNSS
Sbjct: 241 IVLAWKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTAASNSS 300

Query: 301 EGGGRIMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKE 360
           + GGRIM ASF SL+LGKAD+LLSLM+KTFPELGLKKEDCLEMSW+ESMAF ASGFVS  
Sbjct: 301 KEGGRIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAFMASGFVSAN 360

Query: 361 SLDEVLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMN 420
           ++ ++LL+RTPL++ R+K KSDY T+PIS+T LEG+WERFKI+ELET+QL+LIP+GGKMN
Sbjct: 361 NV-KLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFKIEELETVQLMLIPFGGKMN 420

Query: 421 EISETETPSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNY 480
           EISETETP  +RAGYPI+IGYYVTWQ PDADLRH +W QEL+DYMTPFVSKSPR AY+NY
Sbjct: 421 EISETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINY 480

Query: 481 RDLDMGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPPAKI 540
           +DLD+G  N +D I  SYEEA VWG+ YFGNNFERL++VK KVDPFNFFRHEQSIPPAK+
Sbjct: 481 KDLDIG-TNKEDGIPTSYEEAGVWGHPYFGNNFERLVEVKMKVDPFNFFRHEQSIPPAKM 540

Query: 541 TSCSHI 543
            SC  +
Sbjct: 541 ASCPDV 543

BLAST of Tan0002087 vs. NCBI nr
Match: XP_038895033.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 830.1 bits (2143), Expect = 1.2e-236
Identity = 411/535 (76.82%), Postives = 462/535 (86.36%), Query Frame = 0

Query: 3   FDWSYFSFICLLLV----SYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSY 62
           F   ++ FI LLL+    ++G WG+    N+EDF+HCI+ +S+NNDSNSTLIH PSSSSY
Sbjct: 4   FSSCFYGFISLLLLFSLGTFGCWGY----NMEDFVHCINLHSNNNDSNSTLIHLPSSSSY 63

Query: 63  ISVLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVS 122
             +LNF I N RFS   TP P AIITP+ ASQVQA VICCKSH  QIRTRSGGHDFEG S
Sbjct: 64  SYILNFSICNRRFSDPETPKPVAIITPSHASQVQATVICCKSHGFQIRTRSGGHDFEGRS 123

Query: 123 YVSKVPFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVG 182
           YV+ VPFVLIDLI LNSITI+ +D + WV+SGATVGELYY+IGEKS+ LGFPA   P +G
Sbjct: 124 YVANVPFVLIDLINLNSITINMEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFVPAIG 183

Query: 183 VGGFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGI 242
           VGGFLSGGG GMMVRKYGLAADNVVDAY+VD NG++ +R+SMGEDLFWAIRGGGGGSFGI
Sbjct: 184 VGGFLSGGGLGMMVRKYGLAADNVVDAYVVDENGRVFNRHSMGEDLFWAIRGGGGGSFGI 243

Query: 243 VLAWKLKLVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEG 302
           VLAWKLKLVPVPPIVTSFA++ +WDQN A LIHRWQYVAPEVD++LFI A VTASNSS+G
Sbjct: 244 VLAWKLKLVPVPPIVTSFALHKTWDQNTAHLIHRWQYVAPEVDQDLFITAWVTASNSSDG 303

Query: 303 GGRIMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESL 362
            GRIM ASF SLYLGKA ELLSLME+TFPELGLKKEDCLEMSWVESMAFSASGFVS +SL
Sbjct: 304 SGRIMEASFFSLYLGKATELLSLMERTFPELGLKKEDCLEMSWVESMAFSASGFVSAKSL 363

Query: 363 DEVLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEI 422
            E+LLNRTPLN+GR+KIKSDY+TEPISET LEG+WERFK +ELE +QLVLIP+GGKMNEI
Sbjct: 364 -ELLLNRTPLNNGRYKIKSDYLTEPISETALEGMWERFKNEELEKVQLVLIPFGGKMNEI 423

Query: 423 SETETPSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRD 482
           SE+ETP PHRA YPI+IGY VTWQRPDAD RH EW +ELY YMTPFVSKSPRA Y+N  D
Sbjct: 424 SESETPFPHRARYPIQIGYCVTWQRPDADSRHLEWTRELYSYMTPFVSKSPRAVYINNID 483

Query: 483 LDMGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP 534
           LD+G NN +D I  SYEEASVWG+QYFGNNFERL++VKTKVDPFNFFRHEQSIPP
Sbjct: 484 LDLGTNN-KDGIPTSYEEASVWGHQYFGNNFERLMEVKTKVDPFNFFRHEQSIPP 532

BLAST of Tan0002087 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.2e-236
Identity = 409/526 (77.76%), Postives = 460/526 (87.45%), Query Frame = 0

Query: 9   SFICLLLVSYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRN 68
           +FI LLL+S+  WGFG D NLEDFLHCIS +SSN  SNSTLIHTPSSSSY  VLNF IRN
Sbjct: 25  NFISLLLLSFASWGFGSD-NLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRN 84

Query: 69  LRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPFVLI 128
           LRF+   TP P AI+TP +ASQVQA VICCK+H LQIRTRSGGHDFEG SYVS +PFVLI
Sbjct: 85  LRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLI 144

Query: 129 DLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGF 188
           DLI LNSITID  D T W +SGATVGELYYKIGEKSR L FPA I  ++G+GGF SGGG 
Sbjct: 145 DLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGL 204

Query: 189 GMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 248
           GM+VRKYGLA DNVVDAY+VDANGKLLDR+SMGEDLFWAIRGGGGGSFGIVLAWKLKLV 
Sbjct: 205 GMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVR 264

Query: 249 VPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRASFL 308
           VPP VTSFAV+ SWD NAAKLIH WQYVA EVDE+LFI ARVTASNSS+ GGRIM+ASF 
Sbjct: 265 VPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFF 324

Query: 309 SLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRTPL 368
           SL+LGKA EL+SLMEK FPEL LKKEDCLE+SWVE+MAFSAS +VS E+L E+LL+RT L
Sbjct: 325 SLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENL-ELLLDRTSL 384

Query: 369 NSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHR 428
            +GR+K+KSDY T+PISET L GIWERFKI+ELET+QL+LIP+GGKMNEISET+TP+PHR
Sbjct: 385 ITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHR 444

Query: 429 AGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQD 488
           A +PI IGYYVTW RPDAD RH EW +EL+DYMTPFVS+SPRAAY+NY+DLD+G+NN +D
Sbjct: 445 ASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNN-ED 504

Query: 489 EITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPPA 535
            I  SY+EASVWGY+YFG NFERL++VKTKVDPFNFFRHEQSIPPA
Sbjct: 505 GIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPA 547

BLAST of Tan0002087 vs. ExPASy TrEMBL
Match: A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 1.2e-234
Identity = 410/545 (75.23%), Postives = 458/545 (84.04%), Query Frame = 0

Query: 6   SYFSFICLLLVSYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFR 65
           S FSFI L+ VS+  WG G D NL+DFLHCIS +SSN++SNSTLIHTPSSSSY+ VLNF 
Sbjct: 13  SNFSFISLVFVSFASWGCGSD-NLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFS 72

Query: 66  IRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPF 125
           IRNLRF+   TP P AI+TP +AS+VQ  VICCK+H LQIRTRSGG DFEG SYVS VPF
Sbjct: 73  IRNLRFAESDTPKPVAIVTPWQASEVQTTVICCKTHGLQIRTRSGGQDFEGRSYVSNVPF 132

Query: 126 VLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSG 185
           VLIDL  LNSITIDT+D T W +SGATVGELYYKIGEKSR L FPA +   +G+GGF SG
Sbjct: 133 VLIDLTNLNSITIDTEDETAWAQSGATVGELYYKIGEKSRTLAFPAGLVANLGLGGFFSG 192

Query: 186 GGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLK 245
           GGFGM+VRKYGLA DNVVDA +VDANGKLLDR+SMGEDLFWAIRGGGGGSFGIVL WKLK
Sbjct: 193 GGFGMLVRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLK 252

Query: 246 LVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRA 305
           LV VPP VTSFAV+ SWD NAAKLIHRWQYVAPE DE+LFI A V ASNSS+GGGRIM+A
Sbjct: 253 LVRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPEADEDLFITAWVIASNSSDGGGRIMKA 312

Query: 306 SFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNR 365
           SF SL+LG+A EL+SLM K FPEL L KEDCLEMSWVESMAFSAS FVS ESL+ +L   
Sbjct: 313 SFFSLFLGEAAELVSLMAKAFPELELTKEDCLEMSWVESMAFSASVFVSAESLELLLDRT 372

Query: 366 TPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPS 425
           T L++GR+KIKSDY TEPISET L GIWERFKI+ELETLQLVLIP+GGKMNEISET+TP+
Sbjct: 373 TQLSNGRYKIKSDYATEPISETALNGIWERFKIEELETLQLVLIPFGGKMNEISETDTPN 432

Query: 426 PHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNN 485
           PHRA +PI IGYY TW RPDAD RH EW +EL+DYMTPFVSKSPRAAY+N +DLD+GRNN
Sbjct: 433 PHRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNN 492

Query: 486 DQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP--------AKIT 543
            +D I  SYEEAS+WG+QYFG NFERL+ VKTK DPFNFFRHEQSIPP        AK+ 
Sbjct: 493 -EDGIPTSYEEASIWGHQYFGINFERLVAVKTKADPFNFFRHEQSIPPVAPIGGIQAKMA 552

BLAST of Tan0002087 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.5e-234
Identity = 406/543 (74.77%), Postives = 461/543 (84.90%), Query Frame = 0

Query: 6   SYFSFICLLLVSYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFR 65
           S F F+ L+ VS+  WG G D NL+DFLHCIS +SSN++SNSTLIHTPSSSSY+ VLNF 
Sbjct: 13  SNFGFVSLVFVSFASWGCGSD-NLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFS 72

Query: 66  IRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPF 125
           IRNLRF+   TP P AI+TP +ASQVQA VICCK+H LQIRTRSGG DFEG SYVS VPF
Sbjct: 73  IRNLRFAESDTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPF 132

Query: 126 VLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSG 185
           VLIDLI LNSITIDT+D T W +SGATVGELYYKIG+KSR L FPA +   +G+GGF SG
Sbjct: 133 VLIDLINLNSITIDTEDETAWAQSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSG 192

Query: 186 GGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLK 245
           GGFGM++RKYGLA DNVVDA +VDANGKLLDR+SMGEDLFWAIRGGGGGSFGIVL WKLK
Sbjct: 193 GGFGMLLRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLK 252

Query: 246 LVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRA 305
           LV VPP VTSFAV+ SWD NAAKLIH+WQYVAPEVDE+LFI A VTASNSS+GGGRIM+A
Sbjct: 253 LVRVPPTVTSFAVHKSWDLNAAKLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKA 312

Query: 306 SFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNR 365
           SF +L+LG+A EL+SLM K FPEL L +EDCLEMSWVESMAFSAS FVS E+L+ +L   
Sbjct: 313 SFFALFLGEAAELVSLMAKAFPELELTREDCLEMSWVESMAFSASVFVSAENLELLLDRT 372

Query: 366 TPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPS 425
           T L++GR+KIKSDY TEPISET L GIWERFKI+ELETLQL+LIP+GGKMNEISET+TP+
Sbjct: 373 TQLSNGRYKIKSDYATEPISETALNGIWERFKIEELETLQLILIPFGGKMNEISETDTPN 432

Query: 426 PHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNN 485
           PHRA +PI IGYY TW RPDAD RH EW +EL+DYMTPFVSKSPRAAY+N +DLD+GRNN
Sbjct: 433 PHRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNN 492

Query: 486 DQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP--------AKIT 541
            +D I  SYEEAS+WG+QYFG NFERL++VKTK DPFNFFRHEQSIPP        AK+ 
Sbjct: 493 -EDGIPTSYEEASIWGHQYFGINFERLVEVKTKADPFNFFRHEQSIPPVAPIRGIQAKMA 552

BLAST of Tan0002087 vs. ExPASy TrEMBL
Match: A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 1.1e-232
Identity = 406/543 (74.77%), Postives = 463/543 (85.27%), Query Frame = 0

Query: 10  FICLLLVSYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNL 69
           FI LLL+S+  W  G D NL+DFLHCIS +SSN+ SNSTLIHTPSSSSY  VLNF IRNL
Sbjct: 31  FISLLLLSFASWVCGSD-NLQDFLHCISLHSSNHHSNSTLIHTPSSSSYPHVLNFSIRNL 90

Query: 70  RFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPFVLID 129
           RF+   TP P AI+TP + S+VQA VICCK+H LQIRTRSGGHDFEG SYVS VPFVLID
Sbjct: 91  RFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLID 150

Query: 130 LIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFG 189
           LI LNSITID  + T W +SGATVGELYYKIGEKSR L FPA    ++G+GGF SGGG G
Sbjct: 151 LINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGLG 210

Query: 190 MMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPV 249
           M+VRKYGLA DNVVDAY+VDANGKLLDR+SMGEDLFWAIRGGGGGSFGIVLAWKLKLV V
Sbjct: 211 MLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRV 270

Query: 250 PPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSS--EGGGRIMRASF 309
           PP VTSFAV+ SWD NAAKLIHRWQYVAP+VDE+LFI A VTASNSS  EGGGR+M+ASF
Sbjct: 271 PPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMKASF 330

Query: 310 LSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRTP 369
            SL+LGKA EL+SLM+K FPEL LKKEDCLE+SWVE+MAFSAS +VS E+L E+LL+RTP
Sbjct: 331 FSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAFSASEYVSPENL-ELLLDRTP 390

Query: 370 LNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPH 429
           L +GR+K+KSDY T+PISET L GIWERFKI+ELET+QL+LIP+GGKMNEISET TP+PH
Sbjct: 391 LITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETATPNPH 450

Query: 430 RAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQ 489
           RA +PI IGYYVTW RPDAD RH EW +EL+DYMTPFVS+SPRAAY+NY+DLD+GRNN +
Sbjct: 451 RASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRNN-E 510

Query: 490 DEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP--------AKITSC 543
           D I  SY+EASVWGY+YFG NFERL++VKTKVDPFNFF+HE+SIPP        AK+ SC
Sbjct: 511 DGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFQHEESIPPAAPMGVIQAKMASC 570

BLAST of Tan0002087 vs. ExPASy TrEMBL
Match: A0A5D3D0K7 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00130 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 9.0e-227
Identity = 394/508 (77.56%), Postives = 448/508 (88.19%), Query Frame = 0

Query: 29  LEDFLHCISGYSS-NNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPTE 88
           +EDF+ CI  +SS NNDSNSTLIHTPSSSSY  VLNF IRNLRFS   TP P AIITP+ 
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 89  ASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKVPFVLIDLIKLNSITIDTKDNTTWV 148
           ASQVQA VICCKSH LQIRTRSGGHDFEG SYV+ VPFVLIDLI LNSITID +D T WV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 149 ESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAYI 208
           +SGATVGELYYKIGEKSR LGFPA    T+G+GGFLSGGG GMMVRKYGL ADNVVDAY+
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 209 VDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWDQNAA 268
           VD NG++++R SMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP IVTSFA++ +WDQNAA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 269 KLIHRWQYVAPEVDENLFINARVTASNSS-EGGGRIMRASFLSLYLGKADELLSLMEKTF 328
            LI+RWQY+AP VD++LFI+A VTASNSS +  GRIM ASF SL+LG A ELLSLMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 329 PELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRTPLNSGRHKIKSDYVTEPISE 388
           PELGLKK+DCLEMSWVESMAFSASGFV  +SL E+LL+RTP+++GR+K KSDY TEPIS+
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSL-ELLLDRTPVDNGRYKTKSDYATEPISK 360

Query: 389 TVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYVTWQRPDA 448
           T L+G+WERFK +ELET+QL+LIP+GGKMNEISE+ETPSPHRAGYPI IGYY+TWQ+P+A
Sbjct: 361 TALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNA 420

Query: 449 DLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASVWGYQYFG 508
           D R+ EWA+EL+DYMTPFVSKSPRAAYVNYRDLDMG NND D +   Y+EAS+WG++YFG
Sbjct: 421 DSRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNND-DGVPTRYKEASLWGHRYFG 480

Query: 509 NNFERLIDVKTKVDPFNFFRHEQSIPPA 535
           NNFERL++VK KVDPFNFFRHEQSIPPA
Sbjct: 481 NNFERLMEVKMKVDPFNFFRHEQSIPPA 506

BLAST of Tan0002087 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.4e-139
Identity = 249/514 (48.44%), Postives = 345/514 (67.12%), Query Frame = 0

Query: 28  NLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPTE 87
           N E+FL C+S     N+ +S +IHT    SY S+LN  I+N RF  + TP P +IITP +
Sbjct: 33  NHENFLKCLS--HRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQ 92

Query: 88  ASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVSKV-PFVLIDLIKLNSITIDTKDNTTW 147
           A+ VQ+ + C + H + IRTRSGGHD+EG+SY++K  PFV+IDL  L SIT+D  + T W
Sbjct: 93  ATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGW 152

Query: 148 VESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAY 207
           V+SGAT+GELYY+IG+ S+ L FPA + PTVG+GG   GGG+G ++RKYGL+ADNV+DA+
Sbjct: 153 VQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAH 212

Query: 208 IVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWDQNA 267
           IVDANG  LDR  MGED FWAIRGGGG SF +VL+WK++L+ VP +VT F V  + ++ A
Sbjct: 213 IVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEA 272

Query: 268 AKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRASFLSLYLGKADELLSLMEKTF 327
             +I++WQY+A +V  +LFI A +            + ASF  LYLG   +LL+LM+  F
Sbjct: 273 VSIINKWQYIADKVPNDLFIRAMLQKETE-------VYASFPGLYLGPVSDLLALMKDKF 332

Query: 328 PELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRTPLNSGRHKIKSDYVTEPISE 387
           PELGL+  +C EMSW+ES+ +    F+  ES++  +L +    S   K K D++ EPI +
Sbjct: 333 PELGLEIGNCREMSWIESVLW----FIKGESME--ILAKRKRTSRSFKGKDDFIEEPIPK 392

Query: 388 TVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYVTWQRPD- 447
           T ++ +W RF+  E    +++L P+GGKM+EI++ E P PHR G   +I Y   W   + 
Sbjct: 393 TAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEED 452

Query: 448 ----ADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASVWG 507
                  ++  W + +Y++MTP+VSKSPR AYVN+RD+D+G     +  TK YEEA VWG
Sbjct: 453 KNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTK-YEEAKVWG 512

Query: 508 YQYFGNNFERLIDVKTKVDPFNFFRHEQSIPPAK 536
            +YF NNF+RL+ VKT VDP +FF  EQSIP  K
Sbjct: 513 VKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIMK 530

BLAST of Tan0002087 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 489.2 bits (1258), Expect = 4.5e-138
Identity = 256/534 (47.94%), Postives = 351/534 (65.73%), Query Frame = 0

Query: 6   SYFSFICL------LLVSYGRWGFGGDNNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYI 65
           S+FS + +      LL+S         +N   FL C+S   ++++  S +IHTP+ +S+ 
Sbjct: 5   SFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFS 64

Query: 66  SVLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGGHDFEGVSY 125
           SVL   I+N RFS    P P  I+TP + S VQ+AV C +   + IRTRSGGHD+EG+SY
Sbjct: 65  SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 124

Query: 126 VSKVPFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGFPAAIGPTVGV 185
           V+  PFV++DL  L SIT+D  + + WV++GAT+GELYY+IG+K+R L FPA + PTVGV
Sbjct: 125 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 184

Query: 186 GGFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIV 245
           GG  SGGG+G ++RK+GLAAD+V+DA +VDA G++L+R  MGED FWAIRGGGG SF +V
Sbjct: 185 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 244

Query: 246 LAWKLKLVPVPPIVTSFAVYTSWDQNAAKLIHRWQYVAPEVDENLFINARVTASNSSEGG 305
           L+WK+ L+ VP  VT F V    +Q+A K+IHRWQ+VA +V ++LFI   +    +    
Sbjct: 245 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKN---- 304

Query: 306 GRIMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSKESLD 365
             ++RASF  LYLG    LL ++ K FPELGL+++DC EMSW+ES+ + A   + +E ++
Sbjct: 305 --MVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE--LGEEPIN 364

Query: 366 EVLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVLIPYGGKMNEIS 425
            VL  RT   S   K KSD+V EP+ +T +  +W R +  E E  QL+  P+GGKM+EI+
Sbjct: 365 -VLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 424

Query: 426 ETETPSPHRAGYPIKIGYYVTWQRPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDL 485
           + ETP PHR G   +I Y   W R D   ++  W + +YD M+ FV+KSPR AY+N RDL
Sbjct: 425 DYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 484

Query: 486 DMGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIPP 534
           D+G           YEE   WG +YF NNFERL+ VKT VDP +FF  EQSIPP
Sbjct: 485 DLGMYVGVKR--SKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of Tan0002087 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 485.7 bits (1249), Expect = 5.0e-137
Identity = 249/513 (48.54%), Postives = 344/513 (67.06%), Query Frame = 0

Query: 27  NNLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPT 86
           +N EDFL C+S   ++N     +IHT   SS+ S+L+  I+N RFS   TP P +IITP 
Sbjct: 26  SNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPV 85

Query: 87  EASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYVS-KVPFVLIDLIKLNSITIDTKDNTT 146
           +AS VQ  + C + H + +RTRS GH +EG+SY++   PF +IDL  L SI++D  + T 
Sbjct: 86  KASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTG 145

Query: 147 WVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDA 206
           WV++GAT GELYY+IG+ ++ L FPA I PTVGVGG  SGGG+G ++RKYGLAADN++DA
Sbjct: 146 WVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDA 205

Query: 207 YIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWDQN 266
            +VDA+G++LDR +MGED FWAIRGGGG SFG++L+WK+KLV VP  +T F V  +  + 
Sbjct: 206 LVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKE 265

Query: 267 AAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRASFLSLYLGKADELLSLMEKT 326
           A ++I +WQY A +V ++LFI   +  SN +      + A F  LY+G  + LL+LME+ 
Sbjct: 266 AVRIIKKWQYAADKVPDDLFIRTTLERSNKN-----AVHALFTGLYIGPVNNLLALMEEK 325

Query: 327 FPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRTPLNSGRHKIKSDYVTEPIS 386
           FPELGL+KE C EMSW+ES+ + A  F   ESL  VL NR    S   K K D+V EPI 
Sbjct: 326 FPELGLEKEGCEEMSWIESVLWFAD-FPKGESLG-VLTNR-ERTSLSFKGKDDFVQEPIP 385

Query: 387 ETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYVTWQ--- 446
           E  ++ IW R +  E    +++L P+GGKM+E++E ETP PHR G   +I Y   W+   
Sbjct: 386 EAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEE 445

Query: 447 ---RPDADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASV 506
              + + D ++ +W   +Y++MTP+VSKSPR AYVN++D+D+G    + +    YEE   
Sbjct: 446 DKNKTETD-KYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKK--TKYEEGKS 505

Query: 507 WGYQYFGNNFERLIDVKTKVDPFNFFRHEQSIP 533
           WG +YF NNFERL+ VKT+VDP +FF  EQSIP
Sbjct: 506 WGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Tan0002087 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 5.5e-136
Identity = 244/486 (50.21%), Postives = 334/486 (68.72%), Query Frame = 0

Query: 52  TPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPTEASQVQAAVICCKSHRLQIRTRSGG 111
           T + S +  VL    +NLRF   + P P  I +P   S VQA++IC K  R+ +R RSGG
Sbjct: 57  TKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGG 116

Query: 112 HDFEGVSYVSKV--PFVLIDLIKLNSITIDTKDNTTWVESGATVGELYYKIGEKSRVLGF 171
           HD+EG+SYVS++  PF+L+DL K+  + I+ +DN+ WV+SGATVGELYY+I EKS+V GF
Sbjct: 117 HDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGF 176

Query: 172 PAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDAYIVDANGKLLDRNSMGEDLFWAIR 231
           PA +  ++G+GG ++GG +G M+RKYGL ADNV+DA IVDANGKLLDR +MGED FWAIR
Sbjct: 177 PAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIR 236

Query: 232 GGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWDQNAA-KLIHRWQYVAPEVDENLFINA 291
           GG GGSFGI+LAWK+KLVPVP  VT F V  +  Q+   K+I +WQ VA ++ E LFI  
Sbjct: 237 GGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFI-- 296

Query: 292 RVTASNSSEGGGRIMRASFLSLYLGKADELLSLMEKTFPELGLKKEDCLEMSWVESMAFS 351
           RV  + +  GG + +  S+ +L+LG    L+++M+K+FPELGL  +DC+EMSW+ES+A+ 
Sbjct: 297 RVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY- 356

Query: 352 ASGFVSKESLDEVLLNRTPLNSGRHKIKSDYVTEPISETVLEGIWERFKIKELETLQLVL 411
            SGF +    + +L  ++P      K KSD+V  PI E+ L+GI+++   +++    ++ 
Sbjct: 357 ISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIW 416

Query: 412 IPYGGKMNEISETETPSPHRAGYPIKIGYYVTWQRPD-ADLRHWEWAQELYDYMTPFVSK 471
            PYGG M +I E++ P PHR G   K+ Y  +W   D    RH  W ++LY YMTP+VS 
Sbjct: 417 NPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSS 476

Query: 472 SPRAAYVNYRDLDMGRNNDQDEITKSYEEASVWGYQYFGNNFERLIDVKTKVDPFNFFRH 531
           +PR AYVNYRDLD+GRN    ++    ++A VWG  YF NNF RL+ +K KVDP NFFRH
Sbjct: 477 NPREAYVNYRDLDLGRNT--KDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRH 535

Query: 532 EQSIPP 534
           EQSIPP
Sbjct: 537 EQSIPP 535

BLAST of Tan0002087 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 478.0 bits (1229), Expect = 1.0e-134
Identity = 247/508 (48.62%), Postives = 332/508 (65.35%), Query Frame = 0

Query: 28  NLEDFLHCISGYSSNNDSNSTLIHTPSSSSYISVLNFRIRNLRFSGVTTPTPHAIITPTE 87
           N E F  C++  S      S  I    + SY SVL   IRNLRF+  +TP P  II  T 
Sbjct: 24  NSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAATH 83

Query: 88  ASQVQAAVICCKSHRLQIRTRSGGHDFEGVSYV--SKVPFVLIDLIKLNSITIDTKDNTT 147
            S VQAA+ C K H LQ++ RSGGHD++G+SYV  S  PF ++D+  L S+ +D    T 
Sbjct: 84  ESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKTA 143

Query: 148 WVESGATVGELYYKIGEKSRVLGFPAAIGPTVGVGGFLSGGGFGMMVRKYGLAADNVVDA 207
           WV++GA +GE+YY I EKS+ L +PA I PTVGVGG +SGGG+G M+RKYGL  DN +DA
Sbjct: 144 WVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDA 203

Query: 208 YIVDANGKLLDRNSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFAVYTSWDQN 267
            +VD NGK+LDR  MGEDL+WAI GGGGGS+G+VLA+K+ LV VP  VT F +  + +QN
Sbjct: 204 RMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQN 263

Query: 268 AAKLIHRWQYVAPEVDENLFINARVTASNSSEGGGRIMRASFLSLYLGKADELLSLMEKT 327
           A  +IHRWQ VAP++ + LFI   +   N +    + +R +F++++LG    LLS++ + 
Sbjct: 264 ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNRR 323

Query: 328 FPELGLKKEDCLEMSWVESMAFSASGFVSKESLDEVLLNRT-PLNSGRHKIKSDYVTEPI 387
           FPELGL + DC E SW++S+ F  +  +   S + +LL R  P+N    K KSDYV EPI
Sbjct: 324 FPELGLVRSDCTETSWIQSVLFWTN--IQVGSSETLLLQRNQPVN--YLKRKSDYVREPI 383

Query: 388 SETVLEGIWERFKIKELETLQLVLIPYGGKMNEISETETPSPHRAGYPIKIGYYVTWQRP 447
           S T LE IW+  K+ ELE   +   PYGG+M  IS T TP P+RAG   KI Y   W+  
Sbjct: 384 SRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDE 443

Query: 448 DADLRHWEWAQELYDYMTPFVSKSPRAAYVNYRDLDMGRNNDQDEITKSYEEASVWGYQY 507
               R+ E  ++LY +MTPFVSK+PR ++ NYRD+D+G N+   +I+ SY E   +G +Y
Sbjct: 444 TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKIS-SYVEGKRYGKKY 503

Query: 508 FGNNFERLIDVKTKVDPFNFFRHEQSIP 533
           F  NFERL+ +KT+VD  NFFR+EQSIP
Sbjct: 504 FAGNFERLVKIKTRVDSGNFFRNEQSIP 524