Tan0002024 (gene) Snake gourd v1

Overview
NameTan0002024
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionAsparagine synthetase [glutamine-hydrolyzing]
LocationLG05: 3392869 .. 3396838 (-)
RNA-Seq ExpressionTan0002024
SyntenyTan0002024
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACGATTCTTCGTGTTAATCTCTGCAGTTCGTTTCCGTTTTCCTTTTCTAAACTAATTTTGTTATCTTTATTCTTTTTTGAAAATTTCTTCGAGTTTCTTGAGTCGATATGTGTGGAATTCTCGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTCGAACTCTCTCGTCGGCAAGTTCTTAATCTCCTCTGCATTTTCTTCTCTATGCATTTTCTCTCTCATTTCTGTTCTTAATCTGTGTACCTGTCGCTGTCGGTGCTTTATAACTATCTGCGCATTTCAGTTCGATGCAACTTTTTTAACTTTGTAAAATGACAAAACTAAGAAGAAAATGTTAATTTATTTGATGAAAATCCATTTCTCGTCAGTTGTCATCTACGGTCGATTTTTAGGTTTTTTTTTTCCCCTCCAGATATCGGGACTCTTTTTGATACATTCTGTTGTTTTTAGAGTTTGATATATCGTTTTTTCACGCGCCTGTTTGTCAGATTAAGTAGGGAGAGGTGATTTGAAGTTTGAACGATATAATTTTTTCTTTTCCGAGAAAGTATGAACCGTGTTTTAAATCTAGTGTTTGTTAAATAGTGAGCGAAACGAACTCATTTGGCATTGCTTGCCATGCAGTGATTCCGAAAAAAAATTTCTGCCTCTGATTGTGACCTTTTAACTTTTAGATGAGAATCAGATATGATGCTGTGCAACGACGTCGTTGGATAATATGAAGACTACGAAGCAAAAAGAAAACGAAAAGTTGATCTGTTTGATAACTATTTTTTCTTAAAAATTAAGTCTATAAAATTTACTATTTGTTTTTTCTTGTCTTATTATCTATTTTTACAACCAAGTTTTGACCACTAAACTTATTTTTGCTTTTTGAAATTCAGAATTCGTAAAGGAATGGTAAGAAAACTACACTTTTTCAAAATTAAAAATAAAAAAATGGTTATCAACATATTTAATGGAGTAAATTGCAGATTGAAGCACCGTGGCCCAGACTGGAGTGGGCTTTACCAGCACGCTGATTTTTACTTAGCGCATCAACGTTTAGCCATTATCGATCCTGCTTCCGGTGATCAACCTCTCTATAATGAAGATAAGTCCATCGTCGTCACGGTATACTCCCTTACCTTTTTTCTTGGCCTCTTAGTTCCCGCACGATTACACACTCTCTCTTATCTTGCTGACGAGCGAAATTATTTGCTTGAAATCAGGTCAACGGAGAGATATACAACCATGAGGAGCTGAGGGAGAAATTGAGGAACCACAAAATTCGAACGGGCAGTGACTGTGATGTTATCTCACATTTGGTACTAACAGATATGATTATGGGAAAATTTTTTACTCGAATAATCTGACTTTCCATGAGTTACCCATTTGGTGTGGTTCAAGGGAGATGGAATTATGATATTTATCGTTGCTGTCTGTGTAGTATGAAGAATATGGAGAGAATTTTGTGGGCATGCTTGATGGAATGTTCTCTTTCGTGCTATTGGACACTCGTGATAACAGCTTCATTGCTGCTCGAGATGCGATTGGGATTACTTCCCTTTACATTGGCTGGGGACTTGATGGTAACTGAAATAATTCTGAATCCCGTTGGCTCGTCCCTGTGTTTTAATCATTAGATCCATAGACATGTTCTTTAAATACTCGTATTCTGATTATGGCCAGGCTCGGTTTGGATATCTTCGGAACTCAAAGGTCTGAATGATGATTGTGAACATTTTGAGACCTTTCCACCGGGTCACTTGTACTCTAGCAAGGAAGGTGGATTAAAAAGATGGTACAATCCAACCTGGTTCTCTGAAGCTATTCCATCGACTCCATATGATCCACTTGTTCTGAGACGCGCATTGGAAAATGTATGAATTAATTTGAAGATATTTTATCAAGTTAAATTCATTTTGATATTCTATAACTAGGATAATGAGCTTACATGTTTACTTATGCAGGCTGTGATCAAAAGGCTAATGACCGATGTCCCTTTTGGAGTTCTGCTATCTGGTGGCCTAGATTCATCATTGGTTGCCTCTATCACAGCTCGTCACTTAGCTGGCACAAAAGCTGCCAAGCATTGGGGTACAAAACTCCATTCCTTCTGTGTTGGTCTTGAGGTAGGTTTCTTTTCCTTAAGTCTTGCAGTTCCTACAATTATCGGTTTTGTTTTTAGAGAAAAGGATTTGTCTCTGCTATCCAGGGTTCACCAGATCTGAAGGCTGCTAGAGAAGTTGCAGATTTTTTGGGAACTGTCCACCATGAGTTCCATTTCACAGTTCAGGTACAACTACAAGGCTATCTTGAACTTTACAAACAAATATACATCCCACCTTTCCTTGTTCTAGTATTACCTTTTTTATCTTCTTTTATAATAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACAATCAGGGCAAGCACGCCTATGTTTCTTATGGCACGAAAGATTAAGTCACTAGGAGTGAAGATGGTGATTTCTGGTGAAGGCGCTGATGAGATTTTTGGCGGATATCTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGGTATATGATTTTATGAAGGGCGTTGCTCAATTTTTTAATGTTGGGGATGATGAGGGGCGGAATTGCATTAATAACAACTGAGAGCTTTTGTTTTGTTCTTGCTTGCAGATAAAGGCACTTCATTTGTATGATTGCTTAAGAGCAAACAAAGCAACTTCAGCGTGGGGTTTGGAAGTTCGAGTTCCATTTTTAGACAAGGAATTTATTGATGCTGCAATGGCCATTGATCCAGAATGGAAAATGGTAGAGAAATTAATATTCTTCTTAGATTAATAGCCCTTTTTTGTTTCTCATCTAATCTTTTAATCTATTTCAGATCAAACGTGAACAAGGTCGAATTGAAAAATGGGTTCTCAGGAGAGCTTTCGATGATGAACAGAAGCCTTATCTCCCTAAGGTCTGCGTTGGGAATTTCACTTAGTATACAACAACTATAACTTAGATAAGTATGTAACCCTTTTTTCCCGCCTGCAGCATGTTCTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTCAAAGCCCATGCTGCTCAACATGTAATCTGCTAACCCCTTCCCTTCTTTTGCCTTTCGACCCTAGCTATTCGGTGTTCTTTTATTGGAGAGAATGACTTTTCAAACAAGCTCCTAATCATTTGGATATAAATGTTCATATGTTTGATGTTAAATGTTTCAGGTGACCGATAAGATGATGCAGAACGCTGAACACATCTTCCCCCAAAACACTCCAACATCAAAAGAAGCCTACTACTACAGAACCATATTCGAGAGGTTCTTCCCACAGGTAATTTACTTCCATGAACAACAACAATAATGTCGATCCATGTGAGTGAATTTCATTAACCTTGTTTTGCACTTTGGTATATGGCTCTGCAGAACTCAGCTCAACTGACCGTCCCAGGAGGACCAAGCATCGCTTGCAGCACAGCAAAGGCCATCGAGTGGGACGCTGCTTGGTCTAAAAATCTCGATCCTTCAGGTAGAGCTGCGCTCGGAGTTCATGTGTCAGCTTACAAAGACGAGGCAACTGGCGTAAACGGTGTTGTCTCGCCCCAGATAATCGATACCATTCCGAGAATGGAGGTCAGTAGTAGTACATCAGGAGTAGAAATCCTTAGCTAGAATAGTATAATAGTAGTAGTAGGGCTGCTTAGCTTGATATGCCATAAAACCATGTCCTCCAAATTACATGGCGCCATTGTATAAATAACAACACGGGAGCAATGTAAAGTAAGGGTGTTATTATGGGACCTTCCAAGGAAACTTTCTATGAATATGAATATGAATATATATGTATGTGTTAGATTCTTCGGATTATTATGTAATGTTCAGCC

mRNA sequence

AACGATTCTTCGTGTTAATCTCTGCAGTTCGTTTCCGTTTTCCTTTTCTAAACTAATTTTGTTATCTTTATTCTTTTTTGAAAATTTCTTCGAGTTTCTTGAGTCGATATGTGTGGAATTCTCGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTCGAACTCTCTCGTCGATTGAAGCACCGTGGCCCAGACTGGAGTGGGCTTTACCAGCACGCTGATTTTTACTTAGCGCATCAACGTTTAGCCATTATCGATCCTGCTTCCGGTGATCAACCTCTCTATAATGAAGATAAGTCCATCGTCGTCACGGTCAACGGAGAGATATACAACCATGAGGAGCTGAGGGAGAAATTGAGGAACCACAAAATTCGAACGGGCAGTGACTGTGATGTTATCTCACATTTGTATGAAGAATATGGAGAGAATTTTGTGGGCATGCTTGATGGAATGTTCTCTTTCGTGCTATTGGACACTCGTGATAACAGCTTCATTGCTGCTCGAGATGCGATTGGGATTACTTCCCTTTACATTGGCTGGGGACTTGATGGCTCGGTTTGGATATCTTCGGAACTCAAAGGTCTGAATGATGATTGTGAACATTTTGAGACCTTTCCACCGGGTCACTTGTACTCTAGCAAGGAAGGTGGATTAAAAAGATGGTACAATCCAACCTGGTTCTCTGAAGCTATTCCATCGACTCCATATGATCCACTTGTTCTGAGACGCGCATTGGAAAATGCTGTGATCAAAAGGCTAATGACCGATGTCCCTTTTGGAGTTCTGCTATCTGGTGGCCTAGATTCATCATTGGTTGCCTCTATCACAGCTCGTCACTTAGCTGGCACAAAAGCTGCCAAGCATTGGGGTACAAAACTCCATTCCTTCTGTGTTGGTCTTGAGGGTTCACCAGATCTGAAGGCTGCTAGAGAAGTTGCAGATTTTTTGGGAACTGTCCACCATGAGTTCCATTTCACAGTTCAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACAATCAGGGCAAGCACGCCTATGTTTCTTATGGCACGAAAGATTAAGTCACTAGGAGTGAAGATGGTGATTTCTGGTGAAGGCGCTGATGAGATTTTTGGCGGATATCTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATAAAGGCACTTCATTTGTATGATTGCTTAAGAGCAAACAAAGCAACTTCAGCGTGGGGTTTGGAAGTTCGAGTTCCATTTTTAGACAAGGAATTTATTGATGCTGCAATGGCCATTGATCCAGAATGGAAAATGATCAAACGTGAACAAGGTCGAATTGAAAAATGGGTTCTCAGGAGAGCTTTCGATGATGAACAGAAGCCTTATCTCCCTAAGCATGTTCTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTCAAAGCCCATGCTGCTCAACATGTGACCGATAAGATGATGCAGAACGCTGAACACATCTTCCCCCAAAACACTCCAACATCAAAAGAAGCCTACTACTACAGAACCATATTCGAGAGGTTCTTCCCACAGAACTCAGCTCAACTGACCGTCCCAGGAGGACCAAGCATCGCTTGCAGCACAGCAAAGGCCATCGAGTGGGACGCTGCTTGGTCTAAAAATCTCGATCCTTCAGGTAGAGCTGCGCTCGGAGTTCATGTGTCAGCTTACAAAGACGAGGCAACTGGCGTAAACGGTGTTGTCTCGCCCCAGATAATCGATACCATTCCGAGAATGGAGGTCAGTAGTAGTACATCAGGAGTAGAAATCCTTAGCTAGAATAGTATAATAGTAGTAGTAGGGCTGCTTAGCTTGATATGCCATAAAACCATGTCCTCCAAATTACATGGCGCCATTGTATAAATAACAACACGGGAGCAATGTAAAGTAAGGGTGTTATTATGGGACCTTCCAAGGAAACTTTCTATGAATATGAATATGAATATATATGTATGTGTTAGATTCTTCGGATTATTATGTAATGTTCAGCC

Coding sequence (CDS)

ATGTGTGGAATTCTCGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTCGAACTCTCTCGTCGATTGAAGCACCGTGGCCCAGACTGGAGTGGGCTTTACCAGCACGCTGATTTTTACTTAGCGCATCAACGTTTAGCCATTATCGATCCTGCTTCCGGTGATCAACCTCTCTATAATGAAGATAAGTCCATCGTCGTCACGGTCAACGGAGAGATATACAACCATGAGGAGCTGAGGGAGAAATTGAGGAACCACAAAATTCGAACGGGCAGTGACTGTGATGTTATCTCACATTTGTATGAAGAATATGGAGAGAATTTTGTGGGCATGCTTGATGGAATGTTCTCTTTCGTGCTATTGGACACTCGTGATAACAGCTTCATTGCTGCTCGAGATGCGATTGGGATTACTTCCCTTTACATTGGCTGGGGACTTGATGGCTCGGTTTGGATATCTTCGGAACTCAAAGGTCTGAATGATGATTGTGAACATTTTGAGACCTTTCCACCGGGTCACTTGTACTCTAGCAAGGAAGGTGGATTAAAAAGATGGTACAATCCAACCTGGTTCTCTGAAGCTATTCCATCGACTCCATATGATCCACTTGTTCTGAGACGCGCATTGGAAAATGCTGTGATCAAAAGGCTAATGACCGATGTCCCTTTTGGAGTTCTGCTATCTGGTGGCCTAGATTCATCATTGGTTGCCTCTATCACAGCTCGTCACTTAGCTGGCACAAAAGCTGCCAAGCATTGGGGTACAAAACTCCATTCCTTCTGTGTTGGTCTTGAGGGTTCACCAGATCTGAAGGCTGCTAGAGAAGTTGCAGATTTTTTGGGAACTGTCCACCATGAGTTCCATTTCACAGTTCAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACAATCAGGGCAAGCACGCCTATGTTTCTTATGGCACGAAAGATTAAGTCACTAGGAGTGAAGATGGTGATTTCTGGTGAAGGCGCTGATGAGATTTTTGGCGGATATCTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATAAAGGCACTTCATTTGTATGATTGCTTAAGAGCAAACAAAGCAACTTCAGCGTGGGGTTTGGAAGTTCGAGTTCCATTTTTAGACAAGGAATTTATTGATGCTGCAATGGCCATTGATCCAGAATGGAAAATGATCAAACGTGAACAAGGTCGAATTGAAAAATGGGTTCTCAGGAGAGCTTTCGATGATGAACAGAAGCCTTATCTCCCTAAGCATGTTCTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTCAAAGCCCATGCTGCTCAACATGTGACCGATAAGATGATGCAGAACGCTGAACACATCTTCCCCCAAAACACTCCAACATCAAAAGAAGCCTACTACTACAGAACCATATTCGAGAGGTTCTTCCCACAGAACTCAGCTCAACTGACCGTCCCAGGAGGACCAAGCATCGCTTGCAGCACAGCAAAGGCCATCGAGTGGGACGCTGCTTGGTCTAAAAATCTCGATCCTTCAGGTAGAGCTGCGCTCGGAGTTCATGTGTCAGCTTACAAAGACGAGGCAACTGGCGTAAACGGTGTTGTCTCGCCCCAGATAATCGATACCATTCCGAGAATGGAGGTCAGTAGTAGTACATCAGGAGTAGAAATCCTTAGCTAG

Protein sequence

MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGMFSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKREQGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLDPSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS
Homology
[Close]
BLAST of Tan0002024 vs. ExPASy Swiss-Prot
Match: P49078 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 1021.9 bits (2641), Expect = 2.8e-297
Identity = 477/576 (82.81%), Postives = 534/576 (92.71%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRL+HRGPDWSGLYQ+ D YLAHQRLA+IDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDK+IVVTVNGEIYNHEELR++L+NHK RTGSDC+VI+HLYEEYG +FV MLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGWGLDGSVWISSE+KGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK GG K+WYNP WF+E++PSTPY+PL +RRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGTKAAK WG +LHSFCVGLEGSPDLKA +EVA++LGTVHHEFHF+VQDGI
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           K+EFH+E+CRKIKALH YDCLRANK+TSA+GLE RVPFLDK+FI+ AM++DPE KMIK E
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           +GRIEKWVLRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HAAQ+V DKMM N
Sbjct: 421 EGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMMSN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFP NTP +KEAYYYR IFERFFPQNSA+LTVPGG ++ACSTAKA+EWDA+WS N+D
Sbjct: 481 AGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNNMD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRM 577
           PSGRAA+GVH+SAY  +   +  +   + ID +P M
Sbjct: 541 PSGRAAIGVHLSAYDGKNVALT-IPPLKAIDNMPMM 575

[Close]
BLAST of Tan0002024 vs. ExPASy Swiss-Prot
Match: O24661 (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093 GN=AS PE=2 SV=3)

HSP 1 Score: 1021.5 bits (2640), Expect = 3.7e-297
Identity = 481/556 (86.51%), Postives = 518/556 (93.17%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSG++ H D YLAHQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHHGDCYLAHQRLAIVDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDK I VTVNGEIYNHEELR  L NHK RTGSDCDVI+HLYEEYGENFV MLDGM
Sbjct: 61  QPLFNEDKRIAVTVNGEIYNHEELRALLPNHKFRTGSDCDVIAHLYEEYGENFVEMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLD+RDN+FIAARDA GITSLYIGWGLDGSVWISSELKGL+D+CE+FE FPPGH+Y
Sbjct: 121 FSFVLLDSRDNTFIAARDAFGITSLYIGWGLDGSVWISSELKGLHDECENFEVFPPGHVY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK  G +RWYNP WFSEAIPSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKTEGFRRWYNPPWFSEAIPSTPYDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VA++TARHLAGTKAAK WG++LHSFCVGLEGSPDLKA +EVAD+LGTVHHEF FTVQDGI
Sbjct: 241 VAAVTARHLAGTKAAKRWGSQLHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMVISGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRE+CRKIKALH YDCLRANKATSAWGLE RVPFLDKEF++ AM+IDPE KMIK +
Sbjct: 361 KEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFVNLAMSIDPEAKMIKPD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKW+LR+AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA QHVTDKMM N
Sbjct: 421 QGRIEKWILRKAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAEQHVTDKMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFP NTPT+KE YYYR IFERFFPQNSA+LTVPGGPS+ACSTA A+ WDA+WSKNLD
Sbjct: 481 AGHIFPHNTPTTKEGYYYRMIFERFFPQNSAKLTVPGGPSVACSTATAVAWDASWSKNLD 540

Query: 541 PSGRAALGVHVSAYKD 557
           PSGRAA GVH  AY++
Sbjct: 541 PSGRAATGVHDLAYEN 556

[Close]
BLAST of Tan0002024 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 1016.9 bits (2628), Expect = 9.1e-296
Identity = 477/554 (86.10%), Postives = 519/554 (93.68%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL QH D +L+HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDKSIVVTVNGEIYNHEELR +L +HK RTGSDC+VI+HLYEE+GE+FV MLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTR+N F+AARDA+GIT LYIGWGLDGSVW+SSE+KGLNDDCEHFE FPPG+LY
Sbjct: 121 FSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGNLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SS+ G  +RWYNP W++E IPS PYDPLVLR+A E+AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VA++TARHLAG+KAA+ WGT+LHSFCVGLEGSPDLKAA+EVA++LGTVHHEFHFTVQDGI
Sbjct: 241 VAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVI+HIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFH E+CRKIKALH YDCLRANKATSAWGLE RVPFLDKEF+D AM+IDPE KMIK +
Sbjct: 361 KEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVAMSIDPESKMIKPD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
            GRIEKWVLR+AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHAA+HVTD+MM N
Sbjct: 421 LGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAKHVTDRMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A  I+P NTPT+KEAYYYR IFERFFPQNSA+ TVPGGPSIACSTAKAIEWDA WS NLD
Sbjct: 481 AARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAKAIEWDARWSNNLD 540

Query: 541 PSGRAALGVHVSAY 555
           PSGRAALGVH SAY
Sbjct: 541 PSGRAALGVHDSAY 554

[Close]
BLAST of Tan0002024 vs. ExPASy Swiss-Prot
Match: P49092 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=AS1 PE=2 SV=2)

HSP 1 Score: 1010.7 bits (2612), Expect = 6.5e-294
Identity = 478/589 (81.15%), Postives = 537/589 (91.17%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSD +QAKRVRVLELSRRLKHRGPDWSGL+QH D YLAHQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDKSI+VTVNGEIYNHEELR++L NH+ RTGSDCDVI+HLYEE+GENF+ MLDG+
Sbjct: 61  QPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLDGI 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDN+FI ARDAIG+TSLYIGWGLDGSVWISSE+KGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SS+E   +RWYNPTWFSE+IPS PYDPL +R A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASIT+R+LA TKAA+ WG+KLHSFCVGLEGSPDLKAA+EVAD+LGTVHHEF FTVQDGI
Sbjct: 241 VASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVK VISGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFH E+CRKIKALH YDCLRANK+T AWGLE RVPFLDKEFI+ AM IDPE+KMIKR+
Sbjct: 361 KEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYKMIKRD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           +GRIEK++LRRAFDDE+KPYLPKH+LYRQKEQFSDGVGYSWIDGLK HAA+HVTDKM+ N
Sbjct: 421 EGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVTDKMILN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A +IF  NTP +KEAYYYR IFERFFPQNSA+LTVPGGP++ACSTAKA+EWDAAWS NLD
Sbjct: 481 AGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAAWSNNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVH+SAY D+    N +++ + ++    + + + + GV I S
Sbjct: 541 PSGRAALGVHLSAYDDKQ---NNLINNKPVEFEKLIPMEAPSLGVAIHS 586

[Close]
BLAST of Tan0002024 vs. ExPASy Swiss-Prot
Match: P49093 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=AS2 PE=2 SV=2)

HSP 1 Score: 1008.4 bits (2606), Expect = 3.2e-293
Identity = 483/590 (81.86%), Postives = 533/590 (90.34%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGL+QH D +LAHQRLAI+DPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDNFLAHQRLAIVDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NED+SI+VTVNGEI+NHEELR++L NHK RTG DCDVI+HLYEE+GENFV MLDG+
Sbjct: 61  QPLFNEDQSIIVTVNGEIFNHEELRKQLPNHKFRTGCDCDVIAHLYEEHGENFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIG+TSLYIG+GLDGSVWI+SELKGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDGSVWIASELKGLNDDCEHFELFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKE   +RWYNP WFSEAIPS PYDPL LR+A E A+IKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEKEFRRWYNPPWFSEAIPSAPYDPLALRQAFEKAIIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VAS+TAR+LA TKAAK WG+KLHSFCVGLEG+PDLKAAREVAD++GTVHHEF +T+QDGI
Sbjct: 241 VASVTARYLADTKAAKQWGSKLHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYTIQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYH+ETYDVTTIRA TPMFLM+RKIKSLGVKMV+SGEG+DEIF GYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRAGTPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEE H+E+C KIKALH YDCLRANK+T AWGLE RVPFLDK+FID AM IDPE KMIKR+
Sbjct: 361 KEELHQETCSKIKALHKYDCLRANKSTYAWGLEARVPFLDKKFIDVAMGIDPENKMIKRD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           +GRIEKWVLR+AFDDE+ PYLPKH+LYRQKEQFSDGVGY WIDGLK HAA+HVTDKMM N
Sbjct: 421 EGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYGWIDGLKDHAAKHVTDKMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A +I+P NTP +KEAYYYR IFERFFPQNSA+L+VPGG SIACST KAIEWDAAWS NLD
Sbjct: 481 ASNIYPFNTPNTKEAYYYRMIFERFFPQNSARLSVPGGASIACSTEKAIEWDAAWSNNLD 540

Query: 541 PSGRAALGVHVSAYKDEAT-GVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVH SAY D+    V+  V P+ I  IP+MEV  S  GV ILS
Sbjct: 541 PSGRAALGVHDSAYDDQLNKSVSKGVEPEKI--IPKMEV--SPLGVAILS 586

[Close]
BLAST of Tan0002024 vs. NCBI nr
Match: XP_022948084.1 (asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita moschata] >KAG6605406.1 Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 554/589 (94.06%), Postives = 570/589 (96.77%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DFYL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDKSIVVTVNGEIYNHEELR+KL NHK RTGSDCDVI+HLYEEYGENFV MLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFI ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKEGG KRWYNPTWFSEAIPSTPYDPLVLRR+LENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFID AMAIDPEWKMIKRE
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK+VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD+MM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFPQNTPTSKEAYYYRTIFERFFPQN+AQLT+PGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEATGV  VV P I++TIPRMEVS+   GVEILS
Sbjct: 541 PSGRAALGVHVSAYGDEATGVKSVVPPPIVNTIPRMEVSA--PGVEILS 587

[Close]
BLAST of Tan0002024 vs. NCBI nr
Match: XP_022986817.1 (asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita maxima])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 553/589 (93.89%), Postives = 571/589 (96.94%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC+DDS AKRVRVLELSRRLKHRGPDWSGLYQH D YL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCADDSLAKRVRVLELSRRLKHRGPDWSGLYQHGDCYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDKS+VVTVNGEIYNHEELR+KLRNHK RTGSDCDVISHLYEEYGENFVGMLDGM
Sbjct: 61  QPLYNEDKSVVVTVNGEIYNHEELRKKLRNHKFRTGSDCDVISHLYEEYGENFVGMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GG KRWYNPTWFSE IPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGFKRWYNPTWFSEGIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAK WGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKQWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIK LHLYDCLRANKATSAWGLEVRVPFLDKEFI+ AMAIDPEWKM+KRE
Sbjct: 361 KEEFHRESCRKIKELHLYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMVKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK VLYRQKEQFSDGVGYSWIDGLK+HAAQHVTDKMMQN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKSVLYRQKEQFSDGVGYSWIDGLKSHAAQHVTDKMMQN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA+LTVPGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEAT VN ++  ++IDTIPRMEV  STSG++ILS
Sbjct: 541 PSGRAALGVHVSAYADEATNVNSILPSKMIDTIPRMEV--STSGLKILS 587

[Close]
BLAST of Tan0002024 vs. NCBI nr
Match: XP_023007110.1 (asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita maxima])

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 553/589 (93.89%), Postives = 569/589 (96.60%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DFYL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDKSIVVTVNGEIYNHEELR+KL NHK RTGSDCDVI+HLYEEYGENFV MLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFI ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKEGG KRWYNPTWFSEAIPSTPYDPLVLRR+LENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFID AMAIDPEWKMIKRE
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK+VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD+MM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFPQNTPTSKEAYYYRTIFERFFPQN+AQLT+PGGPSIACSTAKA+EWDAAWS NLD
Sbjct: 481 AGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSNNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEATGV  VV P I++TIPRMEVS+   GVEILS
Sbjct: 541 PSGRAALGVHVSAYGDEATGVKSVVPPPIVNTIPRMEVSA--PGVEILS 587

[Close]
BLAST of Tan0002024 vs. NCBI nr
Match: KAG6570648.1 (Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010498.1 Asparagine synthetase [glutamine-hydrolyzing] 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 552/589 (93.72%), Postives = 570/589 (96.77%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC+DDS AKRVRVLELSRRLKHRGPDWSGLYQH D YL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCADDSLAKRVRVLELSRRLKHRGPDWSGLYQHGDCYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDKS+VVTVNGEIYNHEELR+KLRNHK RTGSDCDVISHLYEEYGENFV MLDGM
Sbjct: 61  QPLYNEDKSVVVTVNGEIYNHEELRKKLRNHKFRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GG KRWYNPTWFSE IPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGFKRWYNPTWFSEGIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAK WGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKQWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIK LHLYDCLRANKATSAWGLEVRVPFLDKEFI+ AM+IDPEWKM+KRE
Sbjct: 361 KEEFHRESCRKIKELHLYDCLRANKATSAWGLEVRVPFLDKEFINVAMSIDPEWKMVKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK VLYRQKEQFSDGVGYSWIDGLK+HAAQHVTDKMMQN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKSVLYRQKEQFSDGVGYSWIDGLKSHAAQHVTDKMMQN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA+LTVPGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEAT VN +V  ++IDTIPRMEV  STSG++ILS
Sbjct: 541 PSGRAALGVHVSAYADEATNVNSIVPSKMIDTIPRMEV--STSGLKILS 587

[Close]
BLAST of Tan0002024 vs. NCBI nr
Match: XP_023532195.1 (asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 552/589 (93.72%), Postives = 570/589 (96.77%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DFYL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDKSIVVTVNGEIYNHEELR+KL NHK RTGSDCDVI+HLYEEYGENFV MLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFI ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPG+ Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGNFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKEGG KRWYNPTWFSEAIPSTPYDPLVLRR+LENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFID AMAIDPEWKMIKRE
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK+VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD+MM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFPQNTPTSKEAYYYRTIFERFFPQN+AQLT+PGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEATGV  VV P +++TIPRMEVS+   GVEILS
Sbjct: 541 PSGRAALGVHVSAYGDEATGVKSVVPPPMVNTIPRMEVSA--PGVEILS 587

[Close]
BLAST of Tan0002024 vs. ExPASy TrEMBL
Match: A0A6J1G8T6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=LOC111451773 PE=4 SV=1)

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 554/589 (94.06%), Postives = 570/589 (96.77%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DFYL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDKSIVVTVNGEIYNHEELR+KL NHK RTGSDCDVI+HLYEEYGENFV MLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFI ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKEGG KRWYNPTWFSEAIPSTPYDPLVLRR+LENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFID AMAIDPEWKMIKRE
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK+VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD+MM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFPQNTPTSKEAYYYRTIFERFFPQN+AQLT+PGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEATGV  VV P I++TIPRMEVS+   GVEILS
Sbjct: 541 PSGRAALGVHVSAYGDEATGVKSVVPPPIVNTIPRMEVSA--PGVEILS 587

[Close]
BLAST of Tan0002024 vs. ExPASy TrEMBL
Match: A0A6J1J8M0 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC111484448 PE=4 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 553/589 (93.89%), Postives = 571/589 (96.94%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC+DDS AKRVRVLELSRRLKHRGPDWSGLYQH D YL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCADDSLAKRVRVLELSRRLKHRGPDWSGLYQHGDCYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDKS+VVTVNGEIYNHEELR+KLRNHK RTGSDCDVISHLYEEYGENFVGMLDGM
Sbjct: 61  QPLYNEDKSVVVTVNGEIYNHEELRKKLRNHKFRTGSDCDVISHLYEEYGENFVGMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GG KRWYNPTWFSE IPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGFKRWYNPTWFSEGIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAK WGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKQWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIK LHLYDCLRANKATSAWGLEVRVPFLDKEFI+ AMAIDPEWKM+KRE
Sbjct: 361 KEEFHRESCRKIKELHLYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMVKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK VLYRQKEQFSDGVGYSWIDGLK+HAAQHVTDKMMQN
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKSVLYRQKEQFSDGVGYSWIDGLKSHAAQHVTDKMMQN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA+LTVPGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEAT VN ++  ++IDTIPRMEV  STSG++ILS
Sbjct: 541 PSGRAALGVHVSAYADEATNVNSILPSKMIDTIPRMEV--STSGLKILS 587

[Close]
BLAST of Tan0002024 vs. ExPASy TrEMBL
Match: A0A6J1L231 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC111499708 PE=4 SV=1)

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 553/589 (93.89%), Postives = 569/589 (96.60%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH DFYL HQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDKSIVVTVNGEIYNHEELR+KL NHK RTGSDCDVI+HLYEEYGENFV MLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFI ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKEGG KRWYNPTWFSEAIPSTPYDPLVLRR+LENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFID AMAIDPEWKMIKRE
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPK+VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTD+MM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFPQNTPTSKEAYYYRTIFERFFPQN+AQLT+PGGPSIACSTAKA+EWDAAWS NLD
Sbjct: 481 AGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSNNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVHVSAY DEATGV  VV P I++TIPRMEVS+   GVEILS
Sbjct: 541 PSGRAALGVHVSAYGDEATGVKSVVPPPIVNTIPRMEVSA--PGVEILS 587

[Close]
BLAST of Tan0002024 vs. ExPASy TrEMBL
Match: A0A0A0KF94 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_6G183190 PE=4 SV=1)

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 550/589 (93.38%), Postives = 567/589 (96.26%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH D YLAHQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNED+S+VVTVNGEIYNHEELR+KLRNHKIRTGSDCDVISHLYEEYGENFV MLDGM
Sbjct: 61  QPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKE   KRWYNPTWFSE IPSTPYDPLVLRRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHL GT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALH+YDCLRANKATSAWGLEVRVPFLDKEFI+ AMAIDPEWKMIKR+
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA+LTVPGGPSIACSTAKA+EWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVH +AY DE  GVN V  P+IID+IPRMEV  ST GV+ILS
Sbjct: 541 PSGRAALGVHAAAYGDETNGVNNVAPPKIIDSIPRMEV--STPGVKILS 587

[Close]
BLAST of Tan0002024 vs. ExPASy TrEMBL
Match: A0A1S3CNG7 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103502918 PE=4 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 553/589 (93.89%), Postives = 568/589 (96.43%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQH D YLAHQRLAIIDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDKSIVVTVNGEIYNHEELR+KLRNHKIRTGSDCDVISHLYEEYGENFV MLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSKE   KRWYNPTWFSE IPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHL GT+AAKHWGT+LHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           KEEFHRESCRKIKALH+YDCLRANKATSAWGLEVRVPFLDKEFI+ AMAIDPEWKMIKR+
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           QGRIEKWVLRRAFDDEQ+PYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM N
Sbjct: 421 QGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           AEHIFPQNTPTSKEAYYYRTIFERFFPQNSA+LTVPGGPSIACSTAKAIEWDAAWSKNLD
Sbjct: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRMEVSSSTSGVEILS 590
           PSGRAALGVH +AY DEA GVN +  P+IID+IPRMEV   T GV+ILS
Sbjct: 541 PSGRAALGVHAAAYGDEANGVN-IAPPKIIDSIPRMEV--GTPGVKILS 586

[Close]
BLAST of Tan0002024 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 1021.9 bits (2641), Expect = 2.0e-298
Identity = 477/576 (82.81%), Postives = 534/576 (92.71%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGCSDDSQAKRVRVLELSRRL+HRGPDWSGLYQ+ D YLAHQRLA+IDPASGD
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPL+NEDK+IVVTVNGEIYNHEELR++L+NHK RTGSDC+VI+HLYEEYG +FV MLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGWGLDGSVWISSE+KGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK GG K+WYNP WF+E++PSTPY+PL +RRA ENAVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VASITARHLAGTKAAK WG +LHSFCVGLEGSPDLKA +EVA++LGTVHHEFHF+VQDGI
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIEDVIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           K+EFH+E+CRKIKALH YDCLRANK+TSA+GLE RVPFLDK+FI+ AM++DPE KMIK E
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
           +GRIEKWVLRRAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HAAQ+V DKMM N
Sbjct: 421 EGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMMSN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A HIFP NTP +KEAYYYR IFERFFPQNSA+LTVPGG ++ACSTAKA+EWDA+WS N+D
Sbjct: 481 AGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNNMD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPRM 577
           PSGRAA+GVH+SAY  +   +  +   + ID +P M
Sbjct: 541 PSGRAAIGVHLSAYDGKNVALT-IPPLKAIDNMPMM 575

[Close]
BLAST of Tan0002024 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 968.0 bits (2501), Expect = 3.4e-282
Identity = 440/575 (76.52%), Postives = 515/575 (89.57%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + D YLAH+RLAIIDP SGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDK++ VTVNGEIYNH+ LREKL++H+ RTGSDC+VI+HLYEE+GE F+ MLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SFIAARDAIGIT LYIGWGLDGSVW +SE+K L+DDCE F +FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GGL+RWYNP W++E +PSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VA++  RHL  ++AA+ WG++LH+FC+GL+GSPDLKA REVAD+LGT HHEF FTVQDGI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIE+VIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           K+EFH E+CRKIKALH +DCLRANK+TSAWG+E RVPFLDKEF++ AM+IDPEWK+IK +
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
            GRIEKWVLR AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HV+D M+ N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A  +FP NTP +KEAYYYRTIFE+FFP+++A+ TVPGGPSIACSTAKA+EWDA WSKNLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATWSKNLD 540

Query: 541 PSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPR 576
           PSGRAALGVHV+AY+++           ++D +P+
Sbjct: 541 PSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 575

[Close]
BLAST of Tan0002024 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 965.3 bits (2494), Expect = 2.2e-281
Identity = 442/559 (79.07%), Postives = 506/559 (90.52%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + D YLAH+RLAI+DP SGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDK+I VTVNGEIYNH+ LRE L++H+ RTGSDC+VI+HLYEE+GE FV MLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SFIAARDAIGIT LYIGWGLDGSVW +SE+K L+DDCE F  FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GGL+RWYNP WFSE +PSTPYDPLV+R   E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VAS+  RHL  ++AA  WG+KLH+FC+GL+GSPDLKA REVAD+LGT HHE HFTVQDGI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           K+EFH E+CRKIKALH YDCLRANK+TSAWG+E RVPFLDKEFI+ AM+IDPEWKMI+ +
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
            GRIEKWVLR AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HV++ M+ N
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A  +FP NTP +KEAYYYRTIFE+FFP+++A+ TVPGGPS+ACSTAKA+EWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 541 PSGRAALGVHVSAYKDEAT 560
           PSGRAALGVHVSAY ++ T
Sbjct: 541 PSGRAALGVHVSAYGEDKT 559

[Close]
BLAST of Tan0002024 vs. TAIR 10
Match: AT5G65010.2 (asparagine synthetase 2 )

HSP 1 Score: 964.9 bits (2493), Expect = 2.9e-281
Identity = 441/576 (76.56%), Postives = 515/576 (89.41%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + D YLAH+RLAIIDP SGD
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDK++ VTVNGEIYNH+ LREKL++H+ RTGSDC+VI+HLYEE+GE F+ MLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SFIAARDAIGIT LYIGWGLDGSVW +SE+K L+DDCE F +FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GGL+RWYNP W++E +PSTPYDPLVLR A E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VA++  RHL  ++AA+ WG++LH+FC+GL+GSPDLKA REVAD+LGT HHEF FTVQDGI
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIE+VIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEI GGYLYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           K+EFH E+CRKIKALH +DCLRANK+TSAWG+E RVPFLDKEF++ AM+IDPEWK+IK +
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
            GRIEKWVLR AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HV+D M+ N
Sbjct: 421 LGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTMLSN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFP-QNSAQLTVPGGPSIACSTAKAIEWDAAWSKNL 540
           A  +FP NTP +KEAYYYRTIFE+FFP Q++A+ TVPGGPSIACSTAKA+EWDA WSKNL
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTAKAVEWDATWSKNL 540

Query: 541 DPSGRAALGVHVSAYKDEATGVNGVVSPQIIDTIPR 576
           DPSGRAALGVHV+AY+++           ++D +P+
Sbjct: 541 DPSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 576

[Close]
BLAST of Tan0002024 vs. TAIR 10
Match: AT5G10240.2 (asparagine synthetase 3 )

HSP 1 Score: 954.1 bits (2465), Expect = 5.1e-278
Identity = 439/559 (78.53%), Postives = 504/559 (90.16%), Query Frame = 0 

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGD 60
           MCGILAVLGC D+SQAKR R++ELSRRL+HRGPDWSGL+ + D YLAH+RLAI+DP SGD
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  QPLYNEDKSIVVTVNGEIYNHEELREKLRNHKIRTGSDCDVISHLYEEYGENFVGMLDGM 120
           QPLYNEDK+I VTVNGEIYNH+ LRE L++H+ RTGSDC+VI+HL  ++GE FV MLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SFIAARDAIGIT LYIGWGLDGSVW +SE+K L+DDCE F  FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKEGGLKRWYNPTWFSEAIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240
           SSK+GGL+RWYNP WFSE +PSTPYDPLV+R   E AVIKRLMTDVPFGVLLSGGLDSSL
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 VASITARHLAGTKAAKHWGTKLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300
           VAS+  RHL  ++AA  WG+KLH+FC+GL+GSPDLKA REVAD+LGT HHE HFTVQDGI
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360
           DAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDAAMAIDPEWKMIKRE 420
           K+EFH E+CRKIKALH YDCLRANK+TSAWG+E RVPFLDKEFI+ AM+IDPEWKMI+ +
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

Query: 421 QGRIEKWVLRRAFDDEQKPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQN 480
            GRIEKWVLR AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HV++ M+ N
Sbjct: 421 LGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETMLMN 480

Query: 481 AEHIFPQNTPTSKEAYYYRTIFERFFPQNSAQLTVPGGPSIACSTAKAIEWDAAWSKNLD 540
           A  +FP NTP +KEAYYYRTIFE+FFP+++A+ TVPGGPS+ACSTAKA+EWDAAWS+NLD
Sbjct: 481 ASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQNLD 540

Query: 541 PSGRAALGVHVSAYKDEAT 560
           PSGRAALGVHVSAY ++ T
Sbjct: 541 PSGRAALGVHVSAYGEDKT 558

The following BLAST results are available for this feature:
BLAST of Tan0002024 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P490782.8e-29782.81Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 ... [more]
O246613.7e-29786.51Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093... [more]
P317529.1e-29686.10Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P... [more]
P490926.5e-29481.15Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=A... [more]
P490933.2e-29381.86Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=A... [more]
BLAST of Tan0002024 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022948084.10.0e+0094.06asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita moschata] >KAG66... [more]
XP_022986817.10.0e+0093.89asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita maxima][more]
XP_023007110.10.0e+0093.89asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita maxima][more]
KAG6570648.10.0e+0093.72Asparagine synthetase [glutamine-hydrolyzing] 1, partial [Cucurbita argyrosperma... [more]
XP_023532195.10.0e+0093.72asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucurbita pepo subsp. pepo... [more]
BLAST of Tan0002024 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G8T60.0e+0094.06Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1J8M00.0e+0093.89Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1L2310.0e+0093.89Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0KF940.0e+0093.38Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A1S3CNG70.0e+0093.89Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1035... [more]
BLAST of Tan0002024 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G47340.12.0e-29882.81glutamine-dependent asparagine synthase 1 [more]
AT5G65010.13.4e-28276.52asparagine synthetase 2 [more]
AT5G10240.12.2e-28179.07asparagine synthetase 3 [more]
AT5G65010.22.9e-28176.56asparagine synthetase 2 [more]
AT5G10240.25.1e-27878.53asparagine synthetase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 7..191
e-value: 1.8E-55
score: 189.2
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 24..194
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 194..513
e-value: 9.6E-118
score: 395.3
IPR006426Asparagine synthase, glutamine-hydrolyzingTIGRFAMTIGR01536TIGR01536coord: 22..365
e-value: 9.7E-101
score: 336.2
IPR006426Asparagine synthase, glutamine-hydrolyzingPIRSFPIRSF001589Asn_synthetase_glu-hcoord: 7..360
e-value: 6.3E-98
score: 326.7
coord: 356..515
e-value: 6.7E-30
score: 102.0
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 47..164
e-value: 6.2E-39
score: 132.8
IPR017932Glutamine amidotransferase type 2 domainPROSITEPS51278GATASE_TYPE_2coord: 1..184
score: 29.406303
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 209..359
e-value: 1.7E-57
score: 195.7
IPR001962Asparagine synthaseCDDcd01991Asn_Synthase_B_Ccoord: 210..455
e-value: 4.21107E-84
score: 261.97
NoneNo IPR availablePANTHERPTHR11772:SF43ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]coord: 23..581
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 23..581
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 200..478
IPR033738Asparagine synthase, N-terminal domainCDDcd00712AsnBcoord: 26..191
e-value: 7.99963E-69
score: 220.122

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002024.1Tan0002024.1mRNA
Tan0002024.2Tan0002024.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0006541 glutamine metabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
molecular_function GO:0005524 ATP binding