Tan0001986 (gene) Snake gourd v1

Overview
NameTan0001986
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationLG10: 23677315 .. 23678439 (-)
RNA-Seq ExpressionTan0001986
SyntenyTan0001986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAGAATCAGGCCCTACGGTGGCTGCTCTGCCTCTGCAGGATCGAGTGGCCATTGTGACCGGTGCCTCTCGGGGCATTGGCCGAGCCATCGCCCTTCACCTTGCCTCTCTTGGTGCCCGCCTCGTCGTCAACTACGTCTTCAGTTCAGCCAAAGCTGATCGAGTGGCTGCCGAGATCAACTCATCCTCAACTGCTGGGAGCAGCCAGCGAGCCATTGCTTGGCGGGCGGACGTGTCTGATCCAGAGCAAGTGAAGTCCCTATTTGATGCAGCCGAGCAGGCATTTGGCTCCCAAGTTCATATCCTGGTTAACTCTGCCGGAATTTCAGATCCTACTTACCCTTATATCGCTGATACTTCACTGGAGATTTTCGACCAAATTTTCAGGTCCCTCTTCCTCTTCTCTCATGAAATTTCTTAGGAAAAAGTTGTAATTTTCAAATGGTGGATGAGTTAATTTTAATCTTAAAATCTTTTAATTCTCCTGGAATAGTTTTTAGTTAAATCACCACTAGACCCATAGATGAGTTATGAACTTGGTAAATTTGCATAAGATAAGACTCAACAAGTAGTCATCGATATGAATTAGAAAAGAAAATGACTATAGAGCATCTATAGCTCTGATTGAATATAGAACTTCTAGCTTTATTACCCAGTTAAATTAAGTAGATGAATTATGGTAAATTTTAAATAGATGATTGTGTTTTTCAGCGTGAACACGAGGGGAAGTTTCCTATGCTGCAGAGAAGCAGCAAACAGAGTGAAACACGGCGGTGGGGGGCGGATTATACTGATATCATCGACAGCAGTGGCAGCAGCGACAGCAGGATTAGGGGCGTATACAGCATCGAAGGCGGCGGTGGAGGCAATGACAAAAGTGGTGGCAAAGGAGCTGAGAGGGAGCGGAATAACAGTGAACTGCATAGCACCGGGAGCGACGGCGACGGAGATGTTGTACAAAGGAATGAATGAGGAAGCAGTGAAGAAAGTGATTGAGAAGTGTCCGCTGGGGAGGCTTGGGGATCCAAAAGATGTGGCTTCTTTTGTGGGGTTTTTGGCGTCCGATGATGGCGAGTGGATAAATGGCCAAGTCATGCTCGTCAATGGGGGCATTGTCTAG

mRNA sequence

ATGGCTTCAGAATCAGGCCCTACGGTGGCTGCTCTGCCTCTGCAGGATCGAGTGGCCATTGTGACCGGTGCCTCTCGGGGCATTGGCCGAGCCATCGCCCTTCACCTTGCCTCTCTTGGTGCCCGCCTCGTCGTCAACTACGTCTTCAGTTCAGCCAAAGCTGATCGAGTGGCTGCCGAGATCAACTCATCCTCAACTGCTGGGAGCAGCCAGCGAGCCATTGCTTGGCGGGCGGACGTGTCTGATCCAGAGCAAGTGAAGTCCCTATTTGATGCAGCCGAGCAGGCATTTGGCTCCCAAGTTCATATCCTGGTTAACTCTGCCGGAATTTCAGATCCTACTTACCCTTATATCGCTGATACTTCACTGGAGATTTTCGACCAAATTTTCAGCGTGAACACGAGGGGAAGTTTCCTATGCTGCAGAGAAGCAGCAAACAGAGTGAAACACGGCGGTGGGGGGCGGATTATACTGATATCATCGACAGCAGTGGCAGCAGCGACAGCAGGATTAGGGGCGTATACAGCATCGAAGGCGGCGGTGGAGGCAATGACAAAAGTGGTGGCAAAGGAGCTGAGAGGGAGCGGAATAACAGTGAACTGCATAGCACCGGGAGCGACGGCGACGGAGATGTTGTACAAAGGAATGAATGAGGAAGCAGTGAAGAAAGTGATTGAGAAGTGTCCGCTGGGGAGGCTTGGGGATCCAAAAGATGTGGCTTCTTTTGTGGGGTTTTTGGCGTCCGATGATGGCGAGTGGATAAATGGCCAAGTCATGCTCGTCAATGGGGGCATTGTCTAG

Coding sequence (CDS)

ATGGCTTCAGAATCAGGCCCTACGGTGGCTGCTCTGCCTCTGCAGGATCGAGTGGCCATTGTGACCGGTGCCTCTCGGGGCATTGGCCGAGCCATCGCCCTTCACCTTGCCTCTCTTGGTGCCCGCCTCGTCGTCAACTACGTCTTCAGTTCAGCCAAAGCTGATCGAGTGGCTGCCGAGATCAACTCATCCTCAACTGCTGGGAGCAGCCAGCGAGCCATTGCTTGGCGGGCGGACGTGTCTGATCCAGAGCAAGTGAAGTCCCTATTTGATGCAGCCGAGCAGGCATTTGGCTCCCAAGTTCATATCCTGGTTAACTCTGCCGGAATTTCAGATCCTACTTACCCTTATATCGCTGATACTTCACTGGAGATTTTCGACCAAATTTTCAGCGTGAACACGAGGGGAAGTTTCCTATGCTGCAGAGAAGCAGCAAACAGAGTGAAACACGGCGGTGGGGGGCGGATTATACTGATATCATCGACAGCAGTGGCAGCAGCGACAGCAGGATTAGGGGCGTATACAGCATCGAAGGCGGCGGTGGAGGCAATGACAAAAGTGGTGGCAAAGGAGCTGAGAGGGAGCGGAATAACAGTGAACTGCATAGCACCGGGAGCGACGGCGACGGAGATGTTGTACAAAGGAATGAATGAGGAAGCAGTGAAGAAAGTGATTGAGAAGTGTCCGCTGGGGAGGCTTGGGGATCCAAAAGATGTGGCTTCTTTTGTGGGGTTTTTGGCGTCCGATGATGGCGAGTGGATAAATGGCCAAGTCATGCTCGTCAATGGGGGCATTGTCTAG

Protein sequence

MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEINSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDDGEWINGQVMLVNGGIV
Homology
BLAST of Tan0001986 vs. ExPASy Swiss-Prot
Match: Q9SQR4 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g03980 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.5e-83
Identity = 162/270 (60.00%), Postives = 200/270 (74.07%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           M++ S  +   LPL  RVAIVTG+SRGIGRAIA+HLA LGAR+V+NY   +A A+RVA+E
Sbjct: 1   MSTHSSISQPPLPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASE 60

Query: 61  INS----SSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYP 120
           IN         G   RAI  +A+VS+P QVKS+FDAAE AF + VHILVNSAGI DP YP
Sbjct: 61  INDFPVREEITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYP 120

Query: 121 YIADTSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTA 180
            IADTS+E FD  FSVNT+G+FLC +EAANR+K GGGGRIIL++S+   +   G GAY A
Sbjct: 121 TIADTSVEDFDHTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAA 180

Query: 181 SKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDP 240
           SKAAVE M K++AKEL+G+GIT NC+APG  ATEM + G   E V+K+  + P GR+G+ 
Sbjct: 181 SKAAVETMVKILAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEA 240

Query: 241 KDVASFVGFLASDDGEWINGQVMLVNGGIV 267
           KDV   VGFLA D GEW+NGQ++ VNGG V
Sbjct: 241 KDVVPLVGFLAGDGGEWVNGQIIPVNGGYV 270

BLAST of Tan0001986 vs. ExPASy Swiss-Prot
Match: Q9SQR2 (NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR2 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.7e-79
Identity = 159/269 (59.11%), Postives = 201/269 (74.72%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MA+ S  +   L L  RVAIVTG+SRGIGRAIA+HLA LGAR+VVNY  S  +A++VA  
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 61  INS-----SSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTY 120
           I +     +  AG S R I  +AD+S+P QVKSLFD AE+ F S VHILVNSA I+DP +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 121 PYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYT 180
             I+D S+E+FD+I SVNTRG+F+C REAANR+K GGGGRIIL+S++ V       G+YT
Sbjct: 121 STISDMSVELFDRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYT 180

Query: 181 ASKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGD 240
           ASKAAVEAM K++AKEL+G+ ITVNC++PG  ATEM Y G++ E V+KV  +   GR+G+
Sbjct: 181 ASKAAVEAMAKILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGE 240

Query: 241 PKDVASFVGFLASDDGEWINGQVMLVNGG 265
            KD+A  VGFLASD GEWINGQV++ NGG
Sbjct: 241 TKDIAPVVGFLASDAGEWINGQVIMANGG 269

BLAST of Tan0001986 vs. ExPASy Swiss-Prot
Match: Q08632 (Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 7.7e-69
Identity = 145/256 (56.64%), Postives = 187/256 (73.05%), Query Frame = 0

Query: 12  LPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEINSSS-TAGSS 71
           LPL  RVAIVTGASRGIGR IAL++A  GA++V++Y  +   A+ VA+ IN+ S ++G  
Sbjct: 17  LPLGGRVAIVTGASRGIGREIALNMAEKGAKVVIHYSSNQHAAEEVASIINNKSPSSGDG 76

Query: 72  QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIF 131
            RAI  +ADV++P QV  LFD AE AFG  +HI+VN+AG++D  YP +A TS E +D+IF
Sbjct: 77  VRAIVCKADVAEPSQVAQLFDTAEHAFG-PLHIVVNNAGVTDSKYPTLAQTSDEEWDRIF 136

Query: 132 SVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAAVEAMTKVVAK 191
            VN +G+FLC REAA RV  GGGGRII ISS+ VA      GAYTASKAAVE MT+++A+
Sbjct: 137 QVNCKGAFLCSREAAKRVVRGGGGRIINISSSLVAMPIPRYGAYTASKAAVEMMTRILAQ 196

Query: 192 ELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDD 251
           ELRG+ IT NC+APG  AT+M + G +E AV+  ++  P  RLG  +DVA  V FLASD+
Sbjct: 197 ELRGTQITANCVAPGPVATDMFFAGKSEAAVEAGVKSNPFERLGKVEDVAPLVAFLASDE 256

Query: 252 GEWINGQVMLVNGGIV 267
           GEW+N QV+ VNGG V
Sbjct: 257 GEWVNAQVVRVNGGQV 271

BLAST of Tan0001986 vs. ExPASy Swiss-Prot
Match: P0CU75 (Short chain dehydrogenase claC OS=Passalora fulva OX=5499 GN=claC PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 9.8e-40
Identity = 107/263 (40.68%), Postives = 154/263 (58.56%), Query Frame = 0

Query: 14  LQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEINSSSTAGSSQRA 73
           L  RVA+VTG+ RGIG AIA+HL  LGA +VVNY  S+  A +V  +I      G+   A
Sbjct: 13  LDGRVAVVTGSGRGIGAAIAVHLGRLGANIVVNYANSALDAQKVVDQIK-----GAGSEA 72

Query: 74  IAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIFSVN 133
           IA +AD+ D  Q+  LFD A   FG  V I V+++G+   ++ ++ D + E FD++FS+N
Sbjct: 73  IAIKADIRDVSQIMRLFDEAVAHFG-HVDIAVSNSGV--VSFGHLKDVTEEEFDRVFSLN 132

Query: 134 TRGSFLCCREAANRVKHGGGGRIILISS-TAVAAATAGLGAYTASKAAVEAMTKVVAKEL 193
           TRG F   REA   +    GGRII+ SS T+   +      Y+ SK AV++  ++ +K+ 
Sbjct: 133 TRGQFFVAREAYRHLSE--GGRIIMTSSNTSKDFSVPRHSLYSGSKGAVDSFVRIFSKDC 192

Query: 194 RGSGITVNCIAPGATATEMLY-----------KGMNEEAVKKVIEKCPLGRLGDPKDVAS 253
               ITVN +APG T T+M +           K   E+  +      PL R G P+D+A+
Sbjct: 193 GDKKITVNGVAPGGTVTDMFHDVSHHYIPDGEKYTAEQRQQMAAFASPLHRNGFPEDIAN 252

Query: 254 FVGFLASDDGEWINGQVMLVNGG 265
            VGFLAS +GEWING+V+ ++GG
Sbjct: 253 VVGFLASKEGEWINGKVINLDGG 265

BLAST of Tan0001986 vs. ExPASy Swiss-Prot
Match: P51831 (3-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Bacillus subtilis (strain 168) OX=224308 GN=fabG PE=3 SV=3)

HSP 1 Score: 162.5 bits (410), Expect = 6.4e-39
Identity = 104/253 (41.11%), Postives = 151/253 (59.68%), Query Frame = 0

Query: 14  LQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEINSSSTAGSSQRA 73
           L D+ AIVTGASRGIGR+IAL LA  GA +VVNY  + AKA+ V  EI S       ++A
Sbjct: 2   LNDKTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEIKS-----MGRKA 61

Query: 74  IAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIFSVN 133
           IA +ADVS+PE V+++       F S + ILVN+AGI+      I     + +D + ++N
Sbjct: 62  IAVKADVSNPEDVQNMIKETLSVF-STIDILVNNAGITRDN--LIMRMKEDEWDDVININ 121

Query: 134 TRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAAVEAMTKVVAKELR 193
            +G F C +    ++     GRII +SS    +   G   Y A+KA V  +TK  AKEL 
Sbjct: 122 LKGVFNCTKAVTRQMMKQRSGRIINVSSIVGVSGNPGQANYVAAKAGVIGLTKSSAKELA 181

Query: 194 GSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDDGEW 253
              ITVN IAPG  +T+M  K + ++   +++++ PL R G+P DV+S V FLAS+   +
Sbjct: 182 SRNITVNAIAPGFISTDMTDK-LAKDVQDEMLKQIPLARFGEPSDVSSVVTFLASEGARY 241

Query: 254 INGQVMLVNGGIV 267
           + GQ + ++GG+V
Sbjct: 242 MTGQTLHIDGGMV 245

BLAST of Tan0001986 vs. NCBI nr
Match: XP_023540764.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.0 bits (1177), Expect = 5.6e-125
Identity = 240/266 (90.23%), Postives = 251/266 (94.36%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP VAALPLQDRV IVTGASRGIGRAIA HLA+LGAR+VVNYV SSAKADRVAAE
Sbjct: 1   MASESGPAVAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSS  GS  RAIAWRADVSDPEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATGSGLRAIAWRADVSDPEQVKSLFDTAEQRFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           TSLEIFDQIFSVNTRGSFLCCREAANRVK GGGGRIILISSTAVAAATAG+GAYTASKAA
Sbjct: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKRGGGGRIILISSTAVAAATAGIGAYTASKAA 180

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAMTKV AKE+RGS ITVNCIAPGATATEMLY GM+EEAVK+VIEKCPLGR+G+PKDVA
Sbjct: 181 VEAMTKVAAKEMRGSKITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVGEPKDVA 240

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASD+GEWINGQVMLVNGGIV
Sbjct: 241 SFVGFLASDEGEWINGQVMLVNGGIV 266

BLAST of Tan0001986 vs. NCBI nr
Match: KAG6597707.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 458.0 bits (1177), Expect = 5.6e-125
Identity = 240/266 (90.23%), Postives = 251/266 (94.36%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP +AALPLQDRV IVTGASRGIGRAIA HLA+LGAR+VVNYV SSAKADRVAAE
Sbjct: 1   MASESGPAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSS  GS  RAIAWRADVSDPEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATGSGLRAIAWRADVSDPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           TSLEIFDQIFSVNTRGSFLCCREAANRVK GGGGRIILISSTAVAAATAG+GAYTASKAA
Sbjct: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKRGGGGRIILISSTAVAAATAGIGAYTASKAA 180

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAMTKV AKELRGS ITVNCIAPGATATEMLY GM+EEAVK+VIEKCPLGR+G+PKDVA
Sbjct: 181 VEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVGEPKDVA 240

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASD+GEWINGQVMLVNGGIV
Sbjct: 241 SFVGFLASDEGEWINGQVMLVNGGIV 266

BLAST of Tan0001986 vs. NCBI nr
Match: KAG7029154.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 451.4 bits (1160), Expect = 5.2e-123
Identity = 240/272 (88.24%), Postives = 251/272 (92.28%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP +AALPLQDRV IVTGASRGIGRAIA HLA+LGAR+VVNYV SSAKADRVAAE
Sbjct: 1   MASESGPAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSS  GS  RAIAWRADVSDPEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATGSGLRAIAWRADVSDPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQI------FSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAY 180
           TSLEIFDQI      FSVNTRGSFLCCREAANRVK GGGGRIILISSTAVAAATAG+GAY
Sbjct: 121 TSLEIFDQIFRAIVFFSVNTRGSFLCCREAANRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 181 TASKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTKV AKELRGS ITVNCIAPGATATEMLY GM+EEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 241 DPKDVASFVGFLASDDGEWINGQVMLVNGGIV 267
           +PKDVASFVGFLASD+GEWINGQVMLVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVMLVNGGIV 272

BLAST of Tan0001986 vs. NCBI nr
Match: XP_022932775.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita moschata])

HSP 1 Score: 450.3 bits (1157), Expect = 1.2e-122
Identity = 237/266 (89.10%), Postives = 249/266 (93.61%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP +AALPLQDRV IVTGASRGIGRAIA HLA+LGAR+VVNYV SSAKADRVAAE
Sbjct: 1   MASESGPAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSS  GS  RAIAWRADVS+PEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATGSGLRAIAWRADVSNPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           TSLEIFDQIFSVNTRGSFLCCREAA RVK GGGGRIILISSTAVAAATAG+GAYTASKAA
Sbjct: 121 TSLEIFDQIFSVNTRGSFLCCREAAIRVKRGGGGRIILISSTAVAAATAGIGAYTASKAA 180

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAMTKV AKELRGS ITVNCIAPGATATEMLY GM+EEAVK+VIEKCPLGR+G+PKDVA
Sbjct: 181 VEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVGEPKDVA 240

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASD+GEWINGQV LVNGGIV
Sbjct: 241 SFVGFLASDEGEWINGQVTLVNGGIV 266

BLAST of Tan0001986 vs. NCBI nr
Match: KAA0051280.1 (photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK29981.1 photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa])

HSP 1 Score: 448.0 bits (1151), Expect = 5.8e-122
Identity = 234/266 (87.97%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+VVNYV SSA+AD+V A 
Sbjct: 365 MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 424

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+S AGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 425 INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 484

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           T LEIFD +FSVNTRG FLCC+EAANRVK GGGGRIILISSTAV A TAGLGAYTASKAA
Sbjct: 485 TPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVVATTAGLGAYTASKAA 544

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAM KV AKEL G+GI+VNCIAPGATATEM YKG+NEE VKKVIEKCP+GR+G PKDVA
Sbjct: 545 VEAMAKVAAKELSGTGISVNCIAPGATATEMFYKGINEEGVKKVIEKCPMGRIGVPKDVA 604

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 605 SFVGFLASDDGEWINGQVILVNGGIV 630

BLAST of Tan0001986 vs. ExPASy TrEMBL
Match: A0A6J1F321 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439224 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.7e-123
Identity = 237/266 (89.10%), Postives = 249/266 (93.61%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP +AALPLQDRV IVTGASRGIGRAIA HLA+LGAR+VVNYV SSAKADRVAAE
Sbjct: 1   MASESGPAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSS  GS  RAIAWRADVS+PEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATGSGLRAIAWRADVSNPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           TSLEIFDQIFSVNTRGSFLCCREAA RVK GGGGRIILISSTAVAAATAG+GAYTASKAA
Sbjct: 121 TSLEIFDQIFSVNTRGSFLCCREAAIRVKRGGGGRIILISSTAVAAATAGIGAYTASKAA 180

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAMTKV AKELRGS ITVNCIAPGATATEMLY GM+EEAVK+VIEKCPLGR+G+PKDVA
Sbjct: 181 VEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVGEPKDVA 240

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASD+GEWINGQV LVNGGIV
Sbjct: 241 SFVGFLASDEGEWINGQVTLVNGGIV 266

BLAST of Tan0001986 vs. ExPASy TrEMBL
Match: A0A5A7U7W3 (Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00100 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 2.8e-122
Identity = 234/266 (87.97%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+VVNYV SSA+AD+V A 
Sbjct: 365 MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 424

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+S AGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 425 INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 484

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           T LEIFD +FSVNTRG FLCC+EAANRVK GGGGRIILISSTAV A TAGLGAYTASKAA
Sbjct: 485 TPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVVATTAGLGAYTASKAA 544

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAM KV AKEL G+GI+VNCIAPGATATEM YKG+NEE VKKVIEKCP+GR+G PKDVA
Sbjct: 545 VEAMAKVAAKELSGTGISVNCIAPGATATEMFYKGINEEGVKKVIEKCPMGRIGVPKDVA 604

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 605 SFVGFLASDDGEWINGQVILVNGGIV 630

BLAST of Tan0001986 vs. ExPASy TrEMBL
Match: A0A1S3CP09 (short-chain type dehydrogenase/reductase-like OS=Cucumis melo OX=3656 GN=LOC103502971 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 2.8e-122
Identity = 234/266 (87.97%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+VVNYV SSA+AD+V A 
Sbjct: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+S AGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 61  INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           T LEIFD +FSVNTRG FLCC+EAANRVK GGGGRIILISSTAV A TAGLGAYTASKAA
Sbjct: 121 TPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVVATTAGLGAYTASKAA 180

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAM KV AKEL G+GI+VNCIAPGATATEM YKG+NEE VKKVIEKCP+GR+G PKDVA
Sbjct: 181 VEAMAKVAAKELSGTGISVNCIAPGATATEMFYKGINEEGVKKVIEKCPMGRIGVPKDVA 240

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 241 SFVGFLASDDGEWINGQVILVNGGIV 266

BLAST of Tan0001986 vs. ExPASy TrEMBL
Match: A0A0A0LI83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.8e-122
Identity = 234/266 (87.97%), Postives = 248/266 (93.23%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MASESGP V ALPLQDRVAIVTGASRGIGR IALHLA LGAR+VVNYV SSA+AD+V A+
Sbjct: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSSAEADQVVAD 60

Query: 61  INSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSS AGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIA+
Sbjct: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAN 120

Query: 121 TSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAA 180
           T LEIFD +FSVNTRG FLCC+EAANRVK GGGGRIILISSTAVAA TAGLGAYTASKAA
Sbjct: 121 TPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVAATTAGLGAYTASKAA 180

Query: 181 VEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVA 240
           VEAM KV AKEL G+GI+VNCIAPGATATEM YKG++EE VKKVI+KCP+GRLG PKDVA
Sbjct: 181 VEAMAKVTAKELSGTGISVNCIAPGATATEMFYKGIDEEGVKKVIDKCPMGRLGVPKDVA 240

Query: 241 SFVGFLASDDGEWINGQVMLVNGGIV 267
           SFVGFLASDDGEWINGQV+LVNGGIV
Sbjct: 241 SFVGFLASDDGEWINGQVILVNGGIV 266

BLAST of Tan0001986 vs. ExPASy TrEMBL
Match: A0A6J1DQK1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022892 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 2.0e-104
Identity = 209/272 (76.84%), Postives = 233/272 (85.66%), Query Frame = 0

Query: 1   MASESG---PTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRV 60
           MAS  G   P   ALPLQDRVAIVTGASRGIGR +ALHLA+LGAR+VVNY+ SSA A RV
Sbjct: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60

Query: 61  AAEINSS---STAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPT 120
           AAEINSS     AGS  RA+  +ADVS P++VK+LFD AE+AFGS VHILVNSAGI DPT
Sbjct: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120

Query: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAY 180
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + G+GAY
Sbjct: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180

Query: 181 TASKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTK +AKELRG+GI+ NC+APGATAT+MLY GM+EEAVKKVIEKCPLGRLG
Sbjct: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240

Query: 241 DPKDVASFVGFLASDDGEWINGQVMLVNGGIV 267
            P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 272

BLAST of Tan0001986 vs. TAIR 10
Match: AT3G03980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 3.9e-84
Identity = 162/270 (60.00%), Postives = 200/270 (74.07%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           M++ S  +   LPL  RVAIVTG+SRGIGRAIA+HLA LGAR+V+NY   +A A+RVA+E
Sbjct: 1   MSTHSSISQPPLPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASE 60

Query: 61  INS----SSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYP 120
           IN         G   RAI  +A+VS+P QVKS+FDAAE AF + VHILVNSAGI DP YP
Sbjct: 61  INDFPVREEITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYP 120

Query: 121 YIADTSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTA 180
            IADTS+E FD  FSVNT+G+FLC +EAANR+K GGGGRIIL++S+   +   G GAY A
Sbjct: 121 TIADTSVEDFDHTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAA 180

Query: 181 SKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDP 240
           SKAAVE M K++AKEL+G+GIT NC+APG  ATEM + G   E V+K+  + P GR+G+ 
Sbjct: 181 SKAAVETMVKILAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEA 240

Query: 241 KDVASFVGFLASDDGEWINGQVMLVNGGIV 267
           KDV   VGFLA D GEW+NGQ++ VNGG V
Sbjct: 241 KDVVPLVGFLAGDGGEWVNGQIIPVNGGYV 270

BLAST of Tan0001986 vs. TAIR 10
Match: AT4G13180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 7.4e-83
Identity = 154/257 (59.92%), Postives = 195/257 (75.88%), Query Frame = 0

Query: 10  AALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEINSSSTAGS 69
           ++LPL  RVAIVTGA+RG+GR IA+HL SLGAR+ +NYV SS+KA+ + +E+N SS   S
Sbjct: 9   SSLPLAGRVAIVTGATRGMGREIAIHLHSLGARVTINYVSSSSKAELLVSELNDSSQLKS 68

Query: 70  SQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQI 129
              AIA +ADVSDP+Q+ +LFD  EQ FGS+VHI+VN AG+ DP YP +++T+LE FD  
Sbjct: 69  ---AIAVKADVSDPDQINNLFDQTEQEFGSKVHIVVNCAGVLDPKYPSLSETTLEDFDNT 128

Query: 130 FSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAAVEAMTKVVA 189
           F++NTRGSFLCC+EAA RV  GGGGRII++S++ V     G G Y ASKAAVE M KV+A
Sbjct: 129 FTINTRGSFLCCKEAAKRVMRGGGGRIIMMSTSMVGGLAPGYGVYAASKAAVETMVKVLA 188

Query: 190 KELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVASFVGFLASD 249
           KEL+GS IT NC+APG  ATEM Y G ++E VK +   CP+GR+G+ KD+   VGFLA D
Sbjct: 189 KELKGSRITANCVAPGPVATEMFYAGKSDETVKMLAGACPMGRIGESKDITEIVGFLAGD 248

Query: 250 DGEWINGQVMLVNGGIV 267
            GEWINGQV+  NGG V
Sbjct: 249 GGEWINGQVIRANGGFV 262

BLAST of Tan0001986 vs. TAIR 10
Match: AT5G18210.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 9.0e-81
Identity = 154/238 (64.71%), Postives = 188/238 (78.99%), Query Frame = 0

Query: 14  LQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEINSSSTAGSSQRA 73
           L  RVAIVTG+SRGIGRAIA+HLA LGA++V+NY   S +AD+VAAEINSS+       A
Sbjct: 8   LAGRVAIVTGSSRGIGRAIAIHLAELGAKIVINYTTRSTEADQVAAEINSSAGTVPQPIA 67

Query: 74  IAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIFSVN 133
           + + AD+S+P Q+KSLFDAAE+AF S VHILVNSAGI +P YP IA+T +E FD+IF VN
Sbjct: 68  VVFLADISEPSQIKSLFDAAEKAFNSPVHILVNSAGILNPNYPTIANTPIEEFDRIFKVN 127

Query: 134 TRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAAVEAMTKVVAKELR 193
           TRGSFLCC+EAA R+K GGGGRIIL++S+   A   G GAYTASKAAVEAM K++AKEL+
Sbjct: 128 TRGSFLCCKEAAKRLKRGGGGRIILLTSSLTEALIPGQGAYTASKAAVEAMVKILAKELK 187

Query: 194 GSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVASFVGFLASDDG 252
           G GIT NC++PG  ATEM + G +EE V  +IE+ P GRLG+ KD+AS VGFLASD G
Sbjct: 188 GLGITANCVSPGPVATEMFFDGKSEETVMNIIERSPFGRLGETKDIASVVGFLASDGG 245

BLAST of Tan0001986 vs. TAIR 10
Match: AT3G04000.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.2e-80
Identity = 159/269 (59.11%), Postives = 201/269 (74.72%), Query Frame = 0

Query: 1   MASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAE 60
           MA+ S  +   L L  RVAIVTG+SRGIGRAIA+HLA LGAR+VVNY  S  +A++VA  
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 61  INS-----SSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTY 120
           I +     +  AG S R I  +AD+S+P QVKSLFD AE+ F S VHILVNSA I+DP +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 121 PYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYT 180
             I+D S+E+FD+I SVNTRG+F+C REAANR+K GGGGRIIL+S++ V       G+YT
Sbjct: 121 STISDMSVELFDRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYT 180

Query: 181 ASKAAVEAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGD 240
           ASKAAVEAM K++AKEL+G+ ITVNC++PG  ATEM Y G++ E V+KV  +   GR+G+
Sbjct: 181 ASKAAVEAMAKILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGE 240

Query: 241 PKDVASFVGFLASDDGEWINGQVMLVNGG 265
            KD+A  VGFLASD GEWINGQV++ NGG
Sbjct: 241 TKDIAPVVGFLASDAGEWINGQVIMANGG 269

BLAST of Tan0001986 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-29
Identity = 90/265 (33.96%), Postives = 139/265 (52.45%), Query Frame = 0

Query: 2   ASESGPTVAALPLQDRVAIVTGASRGIGRAIALHLASLGARLVVNYVFSSAKADRVAAEI 61
           A+E  P      ++  V ++TGASRGIG+AIAL L   G +++VNY  S+ +A+ VA +I
Sbjct: 62  ATEQSPGEVVQKVESPVVVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQI 121

Query: 62  NSSSTAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADT 121
                     +AI +  DVS    V ++   A   +G+ + ++VN+AGI+  T   +   
Sbjct: 122 EE-----YGGQAITFGGDVSKATDVDAMMKTALDKWGT-IDVVVNNAGITRDT--LLIRM 181

Query: 122 SLEIFDQIFSVNTRGSFLCCREAANRVKHGGGGRIILISSTAVAAATAGLGAYTASKAAV 181
               +D++ ++N  G FLC + A   +     GRII ISS        G   Y A+K  V
Sbjct: 182 KQSQWDEVIALNLTGVFLCTQAAVKIMMKKKRGRIINISSVVGLIGNIGQANYAAAKGGV 241

Query: 182 EAMTKVVAKELRGSGITVNCIAPGATATEMLYKGMNEEAVKKVIEKCPLGRLGDPKDVAS 241
            + +K  A+E     I VN + PG  A++M  + + E+  KK++   PLGR G  ++VA 
Sbjct: 242 ISFSKTAAREGASRNINVNVVCPGFIASDMTAE-LGEDMEKKILGTIPLGRYGKAEEVAG 301

Query: 242 FVGFLA-SDDGEWINGQVMLVNGGI 266
            V FLA S    +I GQ   ++GGI
Sbjct: 302 LVEFLALSPAASYITGQAFTIDGGI 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SQR45.5e-8360.00NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis th... [more]
Q9SQR21.7e-7959.11NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q086327.7e-6956.64Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1[more]
P0CU759.8e-4040.68Short chain dehydrogenase claC OS=Passalora fulva OX=5499 GN=claC PE=1 SV=1[more]
P518316.4e-3941.113-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Bacillus subtilis (strain 168... [more]
Match NameE-valueIdentityDescription
XP_023540764.15.6e-12590.23NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita pepo s... [more]
KAG6597707.15.6e-12590.23NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbi... [more]
KAG7029154.15.2e-12388.24NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbi... [more]
XP_022932775.11.2e-12289.10NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita moscha... [more]
KAA0051280.15.8e-12287.97photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK2998... [more]
Match NameE-valueIdentityDescription
A0A6J1F3215.7e-12389.10NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita mosc... [more]
A0A5A7U7W32.8e-12287.97Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CP092.8e-12287.97short-chain type dehydrogenase/reductase-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A0A0LI834.8e-12287.97Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1[more]
A0A6J1DQK12.0e-10476.84NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 OS=Mom... [more]
Match NameE-valueIdentityDescription
AT3G03980.13.9e-8460.00NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G13180.17.4e-8359.92NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G18210.19.0e-8164.71NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G04000.11.2e-8059.11NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G24360.11.2e-2933.96NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 154..162
score: 49.84
coord: 99..110
score: 45.56
coord: 174..193
score: 40.33
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 229..249
score: 43.75
coord: 99..110
score: 42.09
coord: 195..212
score: 43.82
coord: 148..164
score: 32.88
coord: 174..193
score: 40.94
coord: 18..35
score: 54.68
NoneNo IPR availableSMARTSM00822PKS_KRcoord: 17..205
e-value: 0.0047
score: -56.8
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 23..265
e-value: 1.9E-60
score: 204.3
NoneNo IPR availableGENE3D3.40.50.720coord: 3..266
e-value: 8.6E-82
score: 276.3
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 14..264
e-value: 4.2E-14
score: 50.1
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 20..221
e-value: 2.2E-14
score: 51.1
NoneNo IPR availablePANTHERPTHR48107NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATEDcoord: 1..266
NoneNo IPR availablePANTHERPTHR48107:SF19SHORT-CHAIN DEHYDROGENASE/REDUCTASEcoord: 1..266
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 161..189
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 15..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001986.1Tan0001986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006303 double-strand break repair via nonhomologous end joining
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0097552 mitochondrial double-strand break repair via homologous recombination
biological_process GO:0007095 mitotic G2 DNA damage checkpoint signaling
biological_process GO:0000723 telomere maintenance
cellular_component GO:0030870 Mre11 complex
cellular_component GO:0035861 site of double-strand break
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity