Tan0001769 (gene) Snake gourd v1

BLAST of Tan0001769 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 3.1e-269
Identity = 479/737 (64.99%), Postives = 567/737 (76.93%), Query Frame = 0

Query: 9   SSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTMLEQVVGS 68
           SSLIFK  FF+L +   +++   SD+DG+  YIVYMG K ED  S  LHHR MLEQVVGS
Sbjct: 3   SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGS 62

Query: 69  TFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFTQ 128
           TFAPE +L++YKRSFNGFAV+L+EEEA+KIA  EGVVSVF N    +HTTRSWDF+GF  
Sbjct: 63  TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPL 122

Query: 129 DVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIGA 188
            V R  QVESNI+VGVLDTGIWPESPSF+D G   P P KWKG C+ S +F+CN+KIIGA
Sbjct: 123 TVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFS-PPPPKWKGTCETSNNFRCNRKIIGA 182

Query: 189 RIYR-SENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAV 248
           R Y        GD+  PRD+ GHGTHTAST AGGLVSQA+LYGLG GTARGGVP ARIA 
Sbjct: 183 RSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAA 242

Query: 249 YKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGILT 308
           YK+CW+DGC D DILAA+DDAIADGVDIISLSVGG   + YF D+IAIG+FHA++ GILT
Sbjct: 243 YKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 302

Query: 309 SNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQY 368
           SNSAGN GP FFTT+++SPW LSVAASTMDRKFVTQVQ+G+G +FQGVSINTFD   + Y
Sbjct: 303 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYY 362

Query: 369 PLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAA-PTLASFNGTVGIV 428
           PL+ GRD PNT  GF+ S SRFC + SV+ NL+ GKI+VC++         S +G  G++
Sbjct: 363 PLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVL 422

Query: 429 MQSTGLKDYASSYPLPASYLRISADNDI-----KLSSTAESTATILKSVAVQDASAPSVV 488
           M S   +DYA SYPLP+S L     ND+      + S     ATI KS  + +ASAP VV
Sbjct: 423 MTS-NTRDYADSYPLPSSVL---DPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVV 482

Query: 489 SFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPH 548
           SFSSRGPN  T D++KPD++ PGVEILAAW  +APV G     R+ L+NIISGTSMSCPH
Sbjct: 483 SFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI---RRNTLFNIISGTSMSCPH 542

Query: 549 ATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLVY 608
            T IA YVKT++PTWSPAAIKSALMTTA  MNA+ NP+AEFAYG+GH+NPLKA+ PGLVY
Sbjct: 543 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 602

Query: 609 NASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSINQF 668
           +A+ESDY+KFLCGQGY T  VR ITGD S CTSGN GRVWDLNYPSF LS +PSQ+ NQ+
Sbjct: 603 DANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQY 662

Query: 669 FTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQAIVSA 728
           F RTLT+V    STY AM+ AP  L I+VNP +LSFNG+GD KSF LTV+G++   +VSA
Sbjct: 663 FNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSA 722

Query: 729 SLVWSNGLHEVRSPITV 739
           SLVWS+G+H VRSPIT+
Sbjct: 723 SLVWSDGVHYVRSPITI 727

BLAST of Tan0001769 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 9.1e-184
Identity = 353/720 (49.03%), Postives = 465/720 (64.58%), Query Frame = 0

Query: 32  SDNDGRK---TYIVYMGSKPEDTSSTPLHHRTMLEQVVGSTFAPEHLLYSYKRSFNGFAV 91
           S ND R+    YIVYMG+ PE   S P HH ++L+++VG+  A   L+ SYKRSFNGFA 
Sbjct: 22  SANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAA 81

Query: 92  RLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFTQDVVRVKQVESNIIVGVLDTG 151
            LS+ E+QK+   + VVSVFP+    + TTRSWDF+GF +   R    ES++IVGV+D+G
Sbjct: 82  NLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSG 141

Query: 152 IWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIGARIYRSENWPQGDIRSPRDSE 211
           IWPES SF+D G   P P KWKG C+    F CN K+IGAR Y           S RD E
Sbjct: 142 IWPESESFDDEGFG-PPPKKWKGSCKGGLKFACNNKLIGARFYNK------FADSARDEE 201

Query: 212 GHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYKICWSDGCYDADILAAFDDA 271
           GHGTHTAST AG  V  AS YGL  GTARGGVPSARIA YK+C+ + C D DILAAFDDA
Sbjct: 202 GHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDA 261

Query: 272 IADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGILTSNSAGNEGPKFFTTSNVSPWS 331
           IADGVD+IS+S+  +   +  N S+AIG+FHAM  GI+T+ SAGN GP   + +NVSPW 
Sbjct: 262 IADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWM 321

Query: 332 LSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQYPLIHGRDAPNTAGGFNSSISR 391
           ++VAAS  DR+F+ +V LG+G    G+S+NTF+L G ++P+++G+   N +   + + + 
Sbjct: 322 ITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQ---NVSRNCSQAQAG 381

Query: 392 FCDENSVDLNLVSGKILVCDSLLAAPTLASFNGTVGIVMQSTGLKDYASSYPLPASYLRI 451
           +C    VD  LV GKI++CD  L     A   G +G+++Q+T L D A   P PAS L  
Sbjct: 382 YCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGF 441

Query: 452 SADNDIK--LSSTAESTATILKSVAVQDASAPSVVSFSSRGPNLVTLDILKPDLTAPGVE 511
                IK  + S     A IL++  + D  AP V SFSSRGP+ V  ++LKPD++APG+E
Sbjct: 442 EDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLE 501

Query: 512 ILAAWSPMAPVSGAV--EDSRSVLYNIISGTSMSCPHATAIAAYVKTFHPTWSPAAIKSA 571
           ILAA+SP+A  S  +  ED RSV Y+++SGTSM+CPH   +AAYVK+FHP WSP+AIKSA
Sbjct: 502 ILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSA 561

Query: 572 LMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLVYNASESDYIKFLCGQGYTTAMVRL 631
           +MTTA  MN K NPE EFAYG+G INP KA +PGLVY     DY+K LC +G+ +  +  
Sbjct: 562 IMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTT 621

Query: 632 ITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSINQFFTRTLTNVEFGVSTYTAMVF-AP 691
            +G N  C+      V DLNYP+     +     N  F RT+TNV F  STY A V    
Sbjct: 622 TSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQ 681

Query: 692 PNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQ--AIVSASLVWSNGLHEVRSPITVYVV 742
           P L+I++ P +L F  + + KSF +T+ G   +  + VS+S+VWS+G H VRSPI  Y +
Sbjct: 682 PELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of Tan0001769 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.6e-175
Identity = 347/751 (46.21%), Postives = 488/751 (64.98%), Query Frame = 0

Query: 13  FKFIFFNLLYGLLVSAGSHSDNDG-RKTYIVYMGSKPEDTSSTPL-HHRTMLEQVVGSTF 72
           F F+F +LL   L S  +  D+ G ++ YIVY+GS P     TP+  H ++L+++ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 73  APEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFTQDV 132
               L+ SYK+SFNGFA RL+E E +++A  E VVSVFP+ K  + TT SW+FMG  + +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 133 --VRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIGA 192
              R + +ES+ I+GV+D+GI+PES SF+D G   P P KWKG C    +F CN K+IGA
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFG-PPPKKWKGTCAGGKNFTCNNKVIGA 186

Query: 193 RIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVY 252
           R Y +++      ++ RD  GHGTHTAS  AG  V+ ++ YGLG GTARGGVP+ARIAVY
Sbjct: 187 RDYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 253 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGILTS 312
           K+C ++GC    +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFHAM  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 313 NSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQYP 372
           N+AGN GPK  T ++ +PW  SVAAS  +R F+ +V LGDG    G S+NT+D+ G  YP
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 373 LIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDS---LLAAPTLASFNGTVGI 432
           L++G+ A  +    +   +R C+   +D  LV GKI++CDS   L+ A  L    G VG 
Sbjct: 367 LVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL----GAVGS 426

Query: 433 VMQSTGLKDYASSYPLPASYLRISADNDIK-----LSSTAESTATILKSVAVQDASAPSV 492
           ++++    D A     P S+L   +++D K     ++ST    AT+LKS  + +  AP V
Sbjct: 427 IVKNPE-PDRAFIRSFPVSFL---SNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLV 486

Query: 493 VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCP 552
            SFSSRGP+ +  DILKPD+TAPGVEILAA+SP +  + +  D+R V Y+++SGTSM+CP
Sbjct: 487 ASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACP 546

Query: 553 HATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVN--PEAEFAYGAGHINPLKAINPG 612
           H   +AAYVKTFHP WSP+ I+SA+MTTA+ MNA  +     EFAYG+GH++P+ AINPG
Sbjct: 547 HVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPG 606

Query: 613 LVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCT---SGNIGRVWDLNYPSFALSTTPS 672
           LVY  +++D+I FLCG  YT+  +R+I+GDNS CT   S  + R  +LNYP+ +   + +
Sbjct: 607 LVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGT 666

Query: 673 QSINQFFTRTLTNVEFGVSTYTAMV--FAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGT 732
           +  N  F RT+TNV    STY A V  F    L I V+P +LS   + + +SF +TV   
Sbjct: 667 KPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSD 726

Query: 733 V--SQAIVSASLVWSNGLHEVRSPITVYVVN 743
              ++  VSA+L+WS+G H VRSPI VY ++
Sbjct: 727 SIGTKQPVSANLIWSDGTHNVRSPIIVYAMS 741

BLAST of Tan0001769 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 2.9e-174
Identity = 349/744 (46.91%), Postives = 473/744 (63.58%), Query Frame = 0

Query: 8   MSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPL-HHRTMLEQVV 67
           M++L       + L  L +S+ S +  D ++ YIVYMGS       TP   H  +L++V 
Sbjct: 1   MATLAASSSLLSCLLVLFLSSVS-AVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVT 60

Query: 68  GSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGF 127
           G +     L+ SYKRSFNGFA RL+E E +++A   GVVSVFPN K  + TT SWDFMG 
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGL 120

Query: 128 TQDV--VRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKK 187
            + +   R   VES+ I+GV+D+GI PES SF+D G   P P KWKG C    +F CN K
Sbjct: 121 KEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFG-PPPQKWKGVCSGGKNFTCNNK 180

Query: 188 IIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSAR 247
           +IGAR Y SE          RD +GHGTHTAST AG  V  AS +G+G GT RGGVP++R
Sbjct: 181 LIGARDYTSE--------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASR 240

Query: 248 IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDG 307
           +A YK+C   GC    +L+AFDDAIADGVD+I++S+G   A  + ND IAIGAFHAM  G
Sbjct: 241 VAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKG 300

Query: 308 ILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLG 367
           +LT NSAGN GPK  + S V+PW L+VAAST +R FVT+V LG+G T  G S+N +++ G
Sbjct: 301 VLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKG 360

Query: 368 KQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASFNGTVG 427
           K YPL++G+ A ++A   ++  +  C+ + VD + V GKILVC        + S  G VG
Sbjct: 361 KDYPLVYGKSAASSA--CDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVG 420

Query: 428 IVMQSTGLKDYASSYPLPASYLRISADNDI--KLSSTAESTATILKSVAVQDASAPSVVS 487
           ++ + T   D A  +PLPA+ L       +   L ST    A +LK+ A+ + ++P + S
Sbjct: 421 LIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIAS 480

Query: 488 FSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPHA 547
           FSSRGPN + +DILKPD+TAPGVEILAA+SP    S   +D+R V Y+++SGTSMSCPH 
Sbjct: 481 FSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHV 540

Query: 548 TAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVN--PEAEFAYGAGHINPLKAINPGLV 607
             +AAYVKTF+P WSP+ I+SA+MTTA+ +NA        EFAYG+GH++P+ A NPGLV
Sbjct: 541 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 600

Query: 608 YNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPS-QSIN 667
           Y   +SD+I FLCG  YT+ ++++I+G+   C+        +LNYPS +   + S  +  
Sbjct: 601 YELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFT 660

Query: 668 QFFTRTLTNVEFGVSTYTAMVFA--PPNLRITVNPPLLSFNGIGDTKSFRLTVQGT--VS 727
             F RTLTNV    STYT+ V A     L + + P +LSF  + + +SF +TV G+   S
Sbjct: 661 VTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDS 720

Query: 728 QAIVSASLVWSNGLHEVRSPITVY 740
           +   SA+L+WS+G H VRSPI VY
Sbjct: 721 EVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of Tan0001769 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 8.5e-174
Identity = 351/752 (46.68%), Postives = 478/752 (63.56%), Query Frame = 0

Query: 8   MSSLIFKFIFFNLLYGLLV--SAGSHSDNDGRKTYIVYMGSKPEDTSSTPL-HHRTMLEQ 67
           M+     +   + ++ LLV   A +  D+  ++ YIVYMG+ P      P+ HH ++L+ 
Sbjct: 1   MTKPAVSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQD 60

Query: 68  VVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFM 127
           V G +   + L+ +YKRSFNGFA RL++ E + +A  + VVSVFPN K  + TT SW+FM
Sbjct: 61  VTGESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFM 120

Query: 128 GFTQD--VVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCN 187
           G  +     R   +ES+ I+GV+D+GI+PES SF+  G   P P KWKG C+   +F  N
Sbjct: 121 GLKESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFG-PPPKKWKGVCKGGKNFTWN 180

Query: 188 KKIIGARIY--RSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGV 247
            K+IGAR Y  + E +P+    S RD  GHG+HTAST AG  V   S YGLG GTARGGV
Sbjct: 181 NKLIGARYYTPKLEGFPE----SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGV 240

Query: 248 PSARIAVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAF 307
           P+ARIAVYK+C    DGC    ILAAFDDAIAD VDII++S+GG+ +  +  D IAIGAF
Sbjct: 241 PAARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAF 300

Query: 308 HAMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSIN 367
           HAM  GIL  NSAGN GP+  T ++++PW  +VAAS  +R FVT+V LG+G T  G S+N
Sbjct: 301 HAMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVN 360

Query: 368 TFDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLAS 427
           +FDL GK+YPL++G+ A ++ G  ++    FC    +D   V GKI++CDS    P  A 
Sbjct: 361 SFDLNGKKYPLVYGKSASSSCGAASAG---FCSPGCLDSKRVKGKIVLCDS-PQNPDEAQ 420

Query: 428 FNGTVGIVMQSTGLKDYASSYPLPASYLRISADNDI--KLSSTAESTATILKSVAVQDAS 487
             G +  +++S    D AS +  P S L     N +   ++ST    A +LKS  + +  
Sbjct: 421 AMGAIASIVRS-HRTDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQR 480

Query: 488 APSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTS 547
           AP V S+ SRGPN +  DILKPD+TAPG EI+AA+SP AP S  + D+R V Y++ +GTS
Sbjct: 481 APVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTS 540

Query: 548 MSCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNP---EAEFAYGAGHINPLK 607
           MSCPH   +AAY+K+FHP WSP+ I+SA+MTTA+ MNA  +P    AEFAYGAGH++P+ 
Sbjct: 541 MSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPIT 600

Query: 608 AINPGLVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRV-WDLNYPSFALST 667
           AI+PGLVY A++SD+I FLCG  YT   +RLI+GD+S CT      +  +LNYPS     
Sbjct: 601 AIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQV 660

Query: 668 TPSQSINQFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQG 727
           + ++     F RT+TNV    +TY A V     L++ V P +LS   + + KSF +T  G
Sbjct: 661 SAAKPFKVIFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVTASG 720

Query: 728 TVSQA--IVSASLVWSNGLHEVRSPITVYVVN 743
              +A  +VSA L+WS+G+H VRSPI VY  N
Sbjct: 721 AGPKAENLVSAQLIWSDGVHFVRSPIVVYATN 738

BLAST of Tan0001769 vs. NCBI nr
Match: XP_038891007.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 626/745 (84.03%), Postives = 670/745 (89.93%), Query Frame = 0

Query: 3   MARRIMSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTML 62
           MARRI+SSLI  FIF NL    LVS+ SHSDND RKTYIVYMG K EDTSSTPLHHRTML
Sbjct: 1   MARRILSSLILNFIFLNLFNCQLVSS-SHSDNDDRKTYIVYMGRKLEDTSSTPLHHRTML 60

Query: 63  EQVVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWD 122
           EQVVGSTFAPEHLLYSYKRSFNGFAV+L++EEAQKIALKEGVVSVFPNGKK++HTTRSWD
Sbjct: 61  EQVVGSTFAPEHLLYSYKRSFNGFAVKLTKEEAQKIALKEGVVSVFPNGKKHVHTTRSWD 120

Query: 123 FMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCN 182
           FMGFTQ V  VKQVESNI+VGVLDTGIWPESPSFND  LD P P  WKG+CQ S  FQCN
Sbjct: 121 FMGFTQSVPHVKQVESNIVVGVLDTGIWPESPSFNDADLD-PPPAGWKGECQISPTFQCN 180

Query: 183 KKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 242
           +KIIGAR+YRSEN+   DI+SPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS
Sbjct: 181 RKIIGARLYRSENFSPEDIQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 240

Query: 243 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMK 302
           ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+E KSYFNDSIAIGAFHAM+
Sbjct: 241 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKSYFNDSIAIGAFHAMQ 300

Query: 303 DGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDL 362
            GILTSNSAGNEGP++FTTSN+SPWSLSVAASTMDRKFV+QVQL +G  +QGV+I+TFDL
Sbjct: 301 HGILTSNSAGNEGPEYFTTSNISPWSLSVAASTMDRKFVSQVQLANGTIYQGVAIHTFDL 360

Query: 363 LGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASF--N 422
           +GKQ+PLI+G DAPN AGGFNSS SRFCDE SVD +LV GKILVCDSLL A T+ SF  N
Sbjct: 361 MGKQHPLIYGGDAPNKAGGFNSSTSRFCDEKSVDPSLVKGKILVCDSLLRASTMESFNNN 420

Query: 423 GTVGIVMQSTGLKDYASSYPLPASYLRISADNDIKLSSTAESTATILKSVAVQDASAPSV 482
           G VGI+MQ + LKDYASSYPLPASYL    + DIKLS    STATI KS A  DASAPSV
Sbjct: 421 GVVGIIMQGSRLKDYASSYPLPASYLH---NEDIKLS----STATIFKSNANLDASAPSV 480

Query: 483 VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCP 542
           VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSP+APVSG   D+RSVLYNIISGTSMSCP
Sbjct: 481 VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPIAPVSGTARDTRSVLYNIISGTSMSCP 540

Query: 543 HATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLV 602
           HATAIA YVKTFHPTWSPAAIKSALMTTAF MN KVNP+AEFAYGAGHINPLKAINPGLV
Sbjct: 541 HATAIAVYVKTFHPTWSPAAIKSALMTTAFSMNGKVNPQAEFAYGAGHINPLKAINPGLV 600

Query: 603 YNASESDYIKFLCG-QGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSIN 662
           YNASE+DYIKFLCG +GYTT MVR ITGDN+ CT  N GRVWDLNYPSFALSTTPSQSIN
Sbjct: 601 YNASETDYIKFLCGHEGYTTDMVRHITGDNTACTPTNSGRVWDLNYPSFALSTTPSQSIN 660

Query: 663 QFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQAIV 722
           QFFTRTLTNVEF  S Y+AMVFAPP+LRITV PPLLSFNGIGDTKSF+LTVQGTVSQAIV
Sbjct: 661 QFFTRTLTNVEFRTSLYSAMVFAPPSLRITVEPPLLSFNGIGDTKSFKLTVQGTVSQAIV 720

Query: 723 SASLVWSNGLHEVRSPITVYVVNKA 745
           SASLVW++G+H+VRSPITVYVVNKA
Sbjct: 721 SASLVWTDGVHQVRSPITVYVVNKA 736

BLAST of Tan0001769 vs. NCBI nr
Match: XP_023548142.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 614/743 (82.64%), Postives = 670/743 (90.17%), Query Frame = 0

Query: 1   MAMARRIMSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRT 60
           MAMARR  SSL+F FIF NL   LLVS+ SHSD+DGRKTYIVYMGSKPED SST L+HRT
Sbjct: 1   MAMARR-KSSLVFNFIFLNLFSCLLVSS-SHSDSDGRKTYIVYMGSKPEDISSTSLYHRT 60

Query: 61  MLEQVVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRS 120
           MLEQVVGS+F+P+HLLYSYKRSFNGFAVRL++EEAQKIAL+EGVVSVFPN KK++HTTRS
Sbjct: 61  MLEQVVGSSFSPKHLLYSYKRSFNGFAVRLTKEEAQKIALEEGVVSVFPNEKKHVHTTRS 120

Query: 121 WDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQ 180
           WDFMGFTQ V RV QVESNI+VGVLDTGIWPESPSFND G+D P P +WKG CQ S DFQ
Sbjct: 121 WDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDEGVD-PPPKEWKGTCQISPDFQ 180

Query: 181 CNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGV 240
           CN+KIIGAR YRSEN P+G++ +PRDS+GHGTHTAS VAGGLV +ASLYGLGFGTARGGV
Sbjct: 181 CNRKIIGARAYRSENLPKGEVVNPRDSKGHGTHTASIVAGGLVRKASLYGLGFGTARGGV 240

Query: 241 PSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHA 300
           PSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG++ KSYFNDSIAIGAFHA
Sbjct: 241 PSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKSYFNDSIAIGAFHA 300

Query: 301 MKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTF 360
           MK GILTSNSAGN GP +FTTSNVSPWSLSVAASTMDRKFV+ VQ G+G   QGV+INTF
Sbjct: 301 MKHGILTSNSAGNRGPNYFTTSNVSPWSLSVAASTMDRKFVSNVQFGNGTLHQGVAINTF 360

Query: 361 DLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASFN 420
           DLL KQYP+IHG DAPN A GFN+S SRFC ENSVDL+LV GKILVCDSLL A T+ SF 
Sbjct: 361 DLLEKQYPMIHGGDAPNKARGFNASTSRFCVENSVDLSLVRGKILVCDSLLRAATMPSFT 420

Query: 421 GTVGIVMQSTGLKDYASSYPLPASYLRISADNDIKLSSTAESTATILKSVAVQDASAPSV 480
           G VGI+MQ T  KDYASSYPLPA+YL I+A NDI+LSS  ESTATIL+S AV+DASAPSV
Sbjct: 421 GAVGIIMQGTREKDYASSYPLPATYLHIAAANDIQLSSPTESTATILQSNAVKDASAPSV 480

Query: 481 VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCP 540
           VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSP+APVSG V DSRSV+YNIISGTSMSCP
Sbjct: 481 VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPIAPVSGVVGDSRSVVYNIISGTSMSCP 540

Query: 541 HATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLV 600
           HATA+A YVKTFHPTWSPAAIKSALMTTAF MN+K+N +AEFAYGAGH+NPLKAINPGLV
Sbjct: 541 HATAMAVYVKTFHPTWSPAAIKSALMTTAFTMNSKLNTQAEFAYGAGHVNPLKAINPGLV 600

Query: 601 YNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSINQ 660
           YNASESDYI  LCGQGYTTAMVRLIT D+S CTS N GRVWDLNYPSFALSTTPS+SI+Q
Sbjct: 601 YNASESDYIDMLCGQGYTTAMVRLITHDSSACTSTNTGRVWDLNYPSFALSTTPSESIHQ 660

Query: 661 FFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQAIVS 720
           FFTRTLTNVE   S YTA VFAPP+LRITV+P +LSFNGIG+TKSFRLTVQGTVSQAIVS
Sbjct: 661 FFTRTLTNVELRASIYTATVFAPPSLRITVDPAVLSFNGIGETKSFRLTVQGTVSQAIVS 720

Query: 721 ASLVWSNGLHEVRSPITVYVVNK 744
           ASLVWS+G+H+VRSPI VYV+NK
Sbjct: 721 ASLVWSDGMHDVRSPIIVYVINK 740

BLAST of Tan0001769 vs. NCBI nr
Match: XP_022139190.1 (LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 597/744 (80.24%), Postives = 651/744 (87.50%), Query Frame = 0

Query: 3   MAR-RIMSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTM 62
           MAR R MSSLIFKFIFF+L Y LLVS GSH DNDGRKTYIVYMGSKPEDT+STPLHHR M
Sbjct: 1   MARSRTMSSLIFKFIFFSLFYRLLVS-GSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAM 60

Query: 63  LEQVVGSTF---APEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTT 122
           LE+V+GSTF   APE LLYSYKRSFNGFAVRL+EEEA K+A KEGVVSVFPNGKK++HTT
Sbjct: 61  LEEVIGSTFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTT 120

Query: 123 RSWDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSAD 182
           RSWDFMGF+Q V R  QVES+++VGVLDTGIWPESPSF D     P P KW G CQ + D
Sbjct: 121 RSWDFMGFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFG-PPPAKWMGACQAADD 180

Query: 183 FQCNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARG 242
           F+CNKKIIGAR+YRSE+ P  DIRSPRDS GHGTHTASTVAGGLV QASLYGLG GTARG
Sbjct: 181 FRCNKKIIGARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARG 240

Query: 243 GVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAF 302
           GVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG+E  SYF+D IAIGAF
Sbjct: 241 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAF 300

Query: 303 HAMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSIN 362
           HAMK GILTSNSAGNEGP +FT SN+SPWSLSVAAST DRK +TQVQLG+GN +QGV+IN
Sbjct: 301 HAMKHGILTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAIN 360

Query: 363 TFDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLAS 422
           TFDLLGKQYPLI+  DAPN AGGFN SISR C ENSVD NLV GKILVCDSLL A  LAS
Sbjct: 361 TFDLLGKQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLAS 420

Query: 423 FNGTVGIVMQSTGLKDYASSYPLPASYLRISADNDIKL--SSTAESTATILKSVAVQDAS 482
           FN TVGIVMQ   LKDYASSYP PASYLR +A +DI+L  SST + TATILKS+AVQDAS
Sbjct: 421 FNATVGIVMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDAS 480

Query: 483 APSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTS 542
           AP VVSFSSRGP  +T DILK DL+APGVEILAAWSP+APV G   DSRS LYNIISGTS
Sbjct: 481 APVVVSFSSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTS 540

Query: 543 MSCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAIN 602
           MSCPHATAIAAYVKTFHPTWSPAA+KSALMTTAF MNAK+NP+AEFAYG+GH+NPLKA+N
Sbjct: 541 MSCPHATAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVN 600

Query: 603 PGLVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQ 662
           PGLVY+A+E DY+KFLCGQGY+TAMVR ITGD+S CT GNIGRVWDLNYPSFALST PS+
Sbjct: 601 PGLVYDANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSR 660

Query: 663 SINQFFTRTLTNVEFGVSTYTAMVFAPPN-LRITVNPPLLSFNGIGDTKSFRLTVQGTVS 722
            INQFF RTLTNVE G STY+A+VF PP+ L ITVNP +LSFNG+GDTKSF LTVQGTVS
Sbjct: 661 PINQFFRRTLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVS 720

Query: 723 QAIVSASLVWSNGLHEVRSPITVY 740
           QAIVSASL WS+G+H+VRSPIT+Y
Sbjct: 721 QAIVSASLEWSDGVHQVRSPITIY 742

BLAST of Tan0001769 vs. NCBI nr
Match: XP_008461717.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 595/749 (79.44%), Postives = 649/749 (86.65%), Query Frame = 0

Query: 1   MAMARR-IMSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHR 60
           M +ARR I+  LI  F+F N L+      GSH DNDGRKTYIVYMGS  EDTSSTPLHHR
Sbjct: 1   MGLARRSIVCFLILNFVFLN-LFNCQPVFGSHLDNDGRKTYIVYMGSTLEDTSSTPLHHR 60

Query: 61  TMLEQVVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTR 120
            MLEQV+GS FAP+HLLYSYKRSFNGFAVRL+EEEAQKIALKEGVVSVFPNGKK++HTTR
Sbjct: 61  AMLEQVIGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTR 120

Query: 121 SWDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADF 180
           SWDFMGFTQ V RV QVESNI+VGVLDTGIWPESPSFND  LD P P  WKG+CQ S DF
Sbjct: 121 SWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDADLD-PPPAGWKGQCQTSPDF 180

Query: 181 QCNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGG 240
           QCN+KIIGAR YRSEN P G+ +SPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGG
Sbjct: 181 QCNRKIIGARTYRSENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGG 240

Query: 241 VPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFH 300
           VPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+  KSYF DSIAIGAFH
Sbjct: 241 VPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFH 300

Query: 301 AMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINT 360
           AMK GILTSNSAGNEGP++FTTSNVSPWSLSVAAST+DRKFV++VQL +G  +QG +I+T
Sbjct: 301 AMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHT 360

Query: 361 FDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASF 420
           FDL+GKQYPLI+G DAPN +GGFNSSISRFCDENSVD +LV GKILVCDS+L A T+ SF
Sbjct: 361 FDLMGKQYPLIYGGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESF 420

Query: 421 --NGTVGIVMQSTGLKDYASSYPLPASYLRISADNDIKLSSTAESTATILKSVAVQDASA 480
             NG VGI+MQ +  KDYASSYPLPASYL          + T  STA+I KS A+ D+SA
Sbjct: 421 NKNGAVGIIMQGSRFKDYASSYPLPASYLH-------NANITLSSTASIFKSNAILDSSA 480

Query: 481 PSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSM 540
           PSVVSFSSRGPNL TLDILKPDLTAPGVEILAAWSP+A VSG   DSRSVLYNIISGTSM
Sbjct: 481 PSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSM 540

Query: 541 SCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINP 600
           SCPHATAIA YVKTF+PTWSPAAIKSALMTTAF MNAKVNP+AEFAYGAGHINPLKA+NP
Sbjct: 541 SCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNP 600

Query: 601 GLVYNASESDYIKFLCGQ-GYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQ 660
           GLVYNA+E+DYI FLCGQ GYTT MVR ITGDN+ CT  N GRVWDLNYPSFA STTPSQ
Sbjct: 601 GLVYNATETDYINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQ 660

Query: 661 -SINQFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVS 720
            +INQFF RTLTNVE   S Y+A VFAPP+LRITV+PP L FNGIGDTKSF+LTVQGTV+
Sbjct: 661 LTINQFFARTLTNVESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVN 720

Query: 721 QAIVSASLVWSNGLHEVRSPITVYVVNKA 745
           Q I+S SLVW++G+H+VRSPITVYV  KA
Sbjct: 721 QNIISGSLVWTDGVHQVRSPITVYVAKKA 740

BLAST of Tan0001769 vs. NCBI nr
Match: XP_031741167.1 (cucumisin [Cucumis sativus])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 580/710 (81.69%), Postives = 630/710 (88.73%), Query Frame = 0

Query: 38  KTYIVYMGSKPEDTSSTPLHHRTMLEQVVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQK 97
           +TYIVYMGSK EDTSSTPLHHR MLEQVVGS FAP+HLLYSYKRSFNGFAVRL+EEEAQK
Sbjct: 19  QTYIVYMGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQK 78

Query: 98  IALKEGVVSVFPNGKKYIHTTRSWDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFN 157
           IALKEGVVSVFPNGKK++HTTRSWDFMGFTQ V RV QVESNI+VGVLDTGIWPESPSFN
Sbjct: 79  IALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFN 138

Query: 158 DTGLDLPLPTKWKGKCQKSADFQCNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTAST 217
           DT L  P P  WKG+CQ S DFQCN+KIIGAR YRSE  P G+I+SPRDSEGHGTHTAST
Sbjct: 139 DTDLG-PPPAGWKGQCQTSPDFQCNRKIIGARTYRSEKLPPGNIQSPRDSEGHGTHTAST 198

Query: 218 VAGGLVSQASLYGLGFGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIIS 277
           VAGGLVS+ASLYGLGFGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIIS
Sbjct: 199 VAGGLVSEASLYGLGFGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIIS 258

Query: 278 LSVGGNEAKSYFNDSIAIGAFHAMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMD 337
           LSVGG+E KSYF DSIAIGAFHA+K GILTSNSAGNEGP++FTTSNVSPWSLSVAAST+D
Sbjct: 259 LSVGGSEVKSYFTDSIAIGAFHAIKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTID 318

Query: 338 RKFVTQVQLGDGNTFQGVSINTFDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDL 397
           RKFV++VQL +G  +QG +I+TFDL+GKQYPLIHG DAPN +GGFNSSISR+C+ENS+DL
Sbjct: 319 RKFVSRVQLANGTVYQGPAIHTFDLMGKQYPLIHGGDAPNKSGGFNSSISRYCNENSLDL 378

Query: 398 NLVSGKILVCDSLLAAPTLASF--NGTVGIVMQSTGLKDYASSYPLPASYLRISADNDIK 457
           +LV GKILVCDS+L A T+ S   NG VGI+MQ +  KDYASSYPLPASYL  +  N   
Sbjct: 379 SLVKGKILVCDSILRASTVESVNKNGAVGIIMQGSRFKDYASSYPLPASYLHSTNIN--- 438

Query: 458 LSSTAESTATILKSVAVQDASAPSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMA 517
              T  STATI KS  + +ASAPSVVSFSSRGPNL TLDILKPDLTAPGVEILAAWSP+A
Sbjct: 439 ---TLSSTATIFKSNEILNASAPSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIA 498

Query: 518 PVSGAVEDSRSVLYNIISGTSMSCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAK 577
           PVSG   DSRSVLYNIISGTSMSCPHATAIA YVKTF+PTWSPAAIKSALMTTAF MNAK
Sbjct: 499 PVSGIAGDSRSVLYNIISGTSMSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAK 558

Query: 578 VNPEAEFAYGAGHINPLKAINPGLVYNASESDYIKFLCGQ-GYTTAMVRLITGDNSGCTS 637
           VNPEAEFAYGAGHINPLKA+NPGLVYNA+E+DYI FLCGQ GYTT MVR ITGD + CT 
Sbjct: 559 VNPEAEFAYGAGHINPLKALNPGLVYNATETDYINFLCGQEGYTTEMVRHITGDKTACTP 618

Query: 638 GNIGRVWDLNYPSFALSTTPSQ-SINQFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPP 697
            N GRVWDLNYPSFA STTPSQ +INQFFTRTLTNVEF  S YTA VFAPP+LRITV+PP
Sbjct: 619 ANSGRVWDLNYPSFAFSTTPSQLTINQFFTRTLTNVEFNTSLYTAKVFAPPSLRITVDPP 678

Query: 698 LLSFNGIGDTKSFRLTVQGTVSQAIVSASLVWSNGLHEVRSPITVYVVNK 744
            L FNGIGDTKSF+LTVQGTV+Q IVS SLVW++G+H+VRSPITVYVV K
Sbjct: 679 SLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVWTDGVHQVRSPITVYVVKK 721

BLAST of Tan0001769 vs. ExPASy TrEMBL
Match: A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 597/744 (80.24%), Postives = 651/744 (87.50%), Query Frame = 0

Query: 3   MAR-RIMSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTM 62
           MAR R MSSLIFKFIFF+L Y LLVS GSH DNDGRKTYIVYMGSKPEDT+STPLHHR M
Sbjct: 1   MARSRTMSSLIFKFIFFSLFYRLLVS-GSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAM 60

Query: 63  LEQVVGSTF---APEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTT 122
           LE+V+GSTF   APE LLYSYKRSFNGFAVRL+EEEA K+A KEGVVSVFPNGKK++HTT
Sbjct: 61  LEEVIGSTFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTT 120

Query: 123 RSWDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSAD 182
           RSWDFMGF+Q V R  QVES+++VGVLDTGIWPESPSF D     P P KW G CQ + D
Sbjct: 121 RSWDFMGFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFG-PPPAKWMGACQAADD 180

Query: 183 FQCNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARG 242
           F+CNKKIIGAR+YRSE+ P  DIRSPRDS GHGTHTASTVAGGLV QASLYGLG GTARG
Sbjct: 181 FRCNKKIIGARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARG 240

Query: 243 GVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAF 302
           GVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG+E  SYF+D IAIGAF
Sbjct: 241 GVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAF 300

Query: 303 HAMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSIN 362
           HAMK GILTSNSAGNEGP +FT SN+SPWSLSVAAST DRK +TQVQLG+GN +QGV+IN
Sbjct: 301 HAMKHGILTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAIN 360

Query: 363 TFDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLAS 422
           TFDLLGKQYPLI+  DAPN AGGFN SISR C ENSVD NLV GKILVCDSLL A  LAS
Sbjct: 361 TFDLLGKQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLAS 420

Query: 423 FNGTVGIVMQSTGLKDYASSYPLPASYLRISADNDIKL--SSTAESTATILKSVAVQDAS 482
           FN TVGIVMQ   LKDYASSYP PASYLR +A +DI+L  SST + TATILKS+AVQDAS
Sbjct: 421 FNATVGIVMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDAS 480

Query: 483 APSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTS 542
           AP VVSFSSRGP  +T DILK DL+APGVEILAAWSP+APV G   DSRS LYNIISGTS
Sbjct: 481 APVVVSFSSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTS 540

Query: 543 MSCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAIN 602
           MSCPHATAIAAYVKTFHPTWSPAA+KSALMTTAF MNAK+NP+AEFAYG+GH+NPLKA+N
Sbjct: 541 MSCPHATAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVN 600

Query: 603 PGLVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQ 662
           PGLVY+A+E DY+KFLCGQGY+TAMVR ITGD+S CT GNIGRVWDLNYPSFALST PS+
Sbjct: 601 PGLVYDANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSR 660

Query: 663 SINQFFTRTLTNVEFGVSTYTAMVFAPPN-LRITVNPPLLSFNGIGDTKSFRLTVQGTVS 722
            INQFF RTLTNVE G STY+A+VF PP+ L ITVNP +LSFNG+GDTKSF LTVQGTVS
Sbjct: 661 PINQFFRRTLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVS 720

Query: 723 QAIVSASLVWSNGLHEVRSPITVY 740
           QAIVSASL WS+G+H+VRSPIT+Y
Sbjct: 721 QAIVSASLEWSDGVHQVRSPITIY 742

BLAST of Tan0001769 vs. ExPASy TrEMBL
Match: A0A1S3CF95 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 595/749 (79.44%), Postives = 649/749 (86.65%), Query Frame = 0

Query: 1   MAMARR-IMSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHR 60
           M +ARR I+  LI  F+F N L+      GSH DNDGRKTYIVYMGS  EDTSSTPLHHR
Sbjct: 1   MGLARRSIVCFLILNFVFLN-LFNCQPVFGSHLDNDGRKTYIVYMGSTLEDTSSTPLHHR 60

Query: 61  TMLEQVVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTR 120
            MLEQV+GS FAP+HLLYSYKRSFNGFAVRL+EEEAQKIALKEGVVSVFPNGKK++HTTR
Sbjct: 61  AMLEQVIGSNFAPKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTR 120

Query: 121 SWDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADF 180
           SWDFMGFTQ V RV QVESNI+VGVLDTGIWPESPSFND  LD P P  WKG+CQ S DF
Sbjct: 121 SWDFMGFTQSVPRVNQVESNIVVGVLDTGIWPESPSFNDADLD-PPPAGWKGQCQTSPDF 180

Query: 181 QCNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGG 240
           QCN+KIIGAR YRSEN P G+ +SPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGG
Sbjct: 181 QCNRKIIGARTYRSENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGG 240

Query: 241 VPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFH 300
           VPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+  KSYF DSIAIGAFH
Sbjct: 241 VPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFH 300

Query: 301 AMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINT 360
           AMK GILTSNSAGNEGP++FTTSNVSPWSLSVAAST+DRKFV++VQL +G  +QG +I+T
Sbjct: 301 AMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHT 360

Query: 361 FDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASF 420
           FDL+GKQYPLI+G DAPN +GGFNSSISRFCDENSVD +LV GKILVCDS+L A T+ SF
Sbjct: 361 FDLMGKQYPLIYGGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESF 420

Query: 421 --NGTVGIVMQSTGLKDYASSYPLPASYLRISADNDIKLSSTAESTATILKSVAVQDASA 480
             NG VGI+MQ +  KDYASSYPLPASYL          + T  STA+I KS A+ D+SA
Sbjct: 421 NKNGAVGIIMQGSRFKDYASSYPLPASYLH-------NANITLSSTASIFKSNAILDSSA 480

Query: 481 PSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSM 540
           PSVVSFSSRGPNL TLDILKPDLTAPGVEILAAWSP+A VSG   DSRSVLYNIISGTSM
Sbjct: 481 PSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSM 540

Query: 541 SCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINP 600
           SCPHATAIA YVKTF+PTWSPAAIKSALMTTAF MNAKVNP+AEFAYGAGHINPLKA+NP
Sbjct: 541 SCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNP 600

Query: 601 GLVYNASESDYIKFLCGQ-GYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQ 660
           GLVYNA+E+DYI FLCGQ GYTT MVR ITGDN+ CT  N GRVWDLNYPSFA STTPSQ
Sbjct: 601 GLVYNATETDYINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQ 660

Query: 661 -SINQFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVS 720
            +INQFF RTLTNVE   S Y+A VFAPP+LRITV+PP L FNGIGDTKSF+LTVQGTV+
Sbjct: 661 LTINQFFARTLTNVESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVN 720

Query: 721 QAIVSASLVWSNGLHEVRSPITVYVVNKA 745
           Q I+S SLVW++G+H+VRSPITVYV  KA
Sbjct: 721 QNIISGSLVWTDGVHQVRSPITVYVAKKA 740

BLAST of Tan0001769 vs. ExPASy TrEMBL
Match: A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 558/739 (75.51%), Postives = 633/739 (85.66%), Query Frame = 0

Query: 9   SSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTMLEQVVGS 68
           SSLIFK +F +LL GLL S+   +++DGRK YIVY+G+KPEDT+STP HH  MLE+VVGS
Sbjct: 3   SSLIFKLVFLSLLCGLLASSLDSNNDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVVGS 62

Query: 69  TFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFTQ 128
           TFAPE LL+SYKRSFNGF V+L+EEEAQKIA KEGVVSVF NGKK++HTTRSWDFMGFT+
Sbjct: 63  TFAPEALLHSYKRSFNGFVVKLTEEEAQKIAAKEGVVSVFLNGKKHLHTTRSWDFMGFTK 122

Query: 129 DVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIGA 188
           +V RVKQVESNI+VGVLD+GIWPESPSF+D G   P P KWKG CQ SA+F CNKKIIGA
Sbjct: 123 NVARVKQVESNIVVGVLDSGIWPESPSFSDLGYG-PPPVKWKGTCQTSANFHCNKKIIGA 182

Query: 189 RIYRSEN-WPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAV 248
           R YRS N +P  DIRSPRDS+GHGTHTASTVAGGLV+QASLYGL  GTARGGVPSARIAV
Sbjct: 183 RAYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAV 242

Query: 249 YKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGILT 308
           YKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+E K YFNDSIAIGAFH+MK GILT
Sbjct: 243 YKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILT 302

Query: 309 SNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQY 368
           SNSAGN+GP +FT  N SPWSLSVAAS++DRKFVT+VQLG+ N +QG +INTFDLLGKQY
Sbjct: 303 SNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFDLLGKQY 362

Query: 369 PLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASFNGTVGIVM 428
           PLI+G DAPN  GGF SS SRFC +N+VD NLV GKILVCD++L+    ASF G VG+VM
Sbjct: 363 PLIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASFXGAVGVVM 422

Query: 429 QSTGLKDYASSYPLPASYLRISADNDIK--LSSTAESTATILKSVAVQDASAPSVVSFSS 488
           Q  G+KD A SYPLPASYL  +A   IK  ++S   +TATILKS AV D SAP VVSFSS
Sbjct: 423 QDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSFSS 482

Query: 489 RGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPHATAI 548
           RGPN  T DILKPDLTAPGVEILAAWSP+AP+SG + DSR  LYNIISGTSMSCPHATA+
Sbjct: 483 RGPNPETFDILKPDLTAPGVEILAAWSPIAPISG-ITDSRKSLYNIISGTSMSCPHATAV 542

Query: 549 AAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLVYNASE 608
           A YVKTFHPTWSPAAIKSALMTTA  +N K+NP+AEFAYGAGH+NPLKA+NPGLVY+A E
Sbjct: 543 AVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDAHE 602

Query: 609 SDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSINQFFTRT 668
           SDY+KFLCGQGYTTAMV+ +T D S CT  N  RVWDLNYPSFALS+TPS+SINQFFTRT
Sbjct: 603 SDYVKFLCGQGYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSESINQFFTRT 662

Query: 669 LTNVEFGVSTYTAMVF-APPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQAIVSASLV 728
           LTNV    STY A V+ APP L I+V+PP+LSF+ IG+ KSF+LT++GT+S +IVSASLV
Sbjct: 663 LTNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTLSHSIVSASLV 722

Query: 729 WSNGLHEVRSPITVYVVNK 744
           W++GLH+VRSPITVYVVNK
Sbjct: 723 WNDGLHQVRSPITVYVVNK 739

BLAST of Tan0001769 vs. ExPASy TrEMBL
Match: A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 543/741 (73.28%), Postives = 624/741 (84.21%), Query Frame = 0

Query: 9   SSLIFKFIFFNLLYGLLVSAG-SHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTMLEQVVG 68
           SSLIFK + F    G L+++G   SDNDGRK YIVY+G+KPED++STP HH  MLE+VVG
Sbjct: 3   SSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVG 62

Query: 69  STFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFT 128
           STFAP+ LL+SYKRSFNGF V+L+EEEAQKI+ KEGVVSVFPNGKK++HTTRSWDFMGFT
Sbjct: 63  STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 122

Query: 129 QDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIG 188
           ++V RVKQVESNI+VGVLD+GIWPESPSF+D G   P P KWKG CQ SA+F+CN+KIIG
Sbjct: 123 KNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYG-PPPAKWKGACQTSANFRCNRKIIG 182

Query: 189 ARIYRSEN-WPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIA 248
           AR YRS+N +P  DI+SPRDS+GHGTHTASTVAGGLV+QASLYGL  GTARGGVPSARIA
Sbjct: 183 ARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 242

Query: 249 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGIL 308
           VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+ K YFNDSIAIGAFH+MK GIL
Sbjct: 243 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 302

Query: 309 TSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQ 368
           TSNSAGN+GP +FT  N SPWSLSVAAS++DRK V++VQLG+ N +QG +INTFDL GKQ
Sbjct: 303 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 362

Query: 369 YPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASFNGTVGIV 428
           YPLI+  +APN +GGF  S SRFC  NSVD NLV GKIL+CDS+L+  T ASFNG VG+V
Sbjct: 363 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 422

Query: 429 MQSTGLKDYASSYPLPASYLRISADNDIK--LSSTAESTATILKSVAVQDASAPSVVSFS 488
           M   G+KD A SYPLP+SYL   A N+IK  + S+   TATI KS AV D SAP +VSFS
Sbjct: 423 MNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSFS 482

Query: 489 SRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPHATA 548
           SRGPN  T DILKPDLTAPGVEILAAWSP+A VS  V DSR+ LYNIISGTSMSCPHATA
Sbjct: 483 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 542

Query: 549 IAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLVYNAS 608
            A YVKTFHP+WSPAAIKSALMTTA  +N K+NP+AEFAYGAGHINP+KA+NPGLVY+A 
Sbjct: 543 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 602

Query: 609 ESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSINQFFTR 668
           ESDY++FLCGQGYTTAMVR ++GD+S CT  N GRVWDLNYPSFALS+TPS+SINQFF R
Sbjct: 603 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFRR 662

Query: 669 TLTNVEFGVSTYTAMVF-APPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQAIVSASL 728
           T+TNV   V+TY A V   P  L I+VNPP+LSFN IG  KSF +TV+G V+Q IVSA+L
Sbjct: 663 TVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAAL 722

Query: 729 VWSNGLHEVRSPITVYVVNKA 745
           +W++G H VRSPITVYVV+KA
Sbjct: 723 LWTDGHHHVRSPITVYVVDKA 742

BLAST of Tan0001769 vs. ExPASy TrEMBL
Match: A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.8e-310
Identity = 544/741 (73.41%), Postives = 621/741 (83.81%), Query Frame = 0

Query: 9   SSLIFKFIFFNLLYG-LLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPLHHRTMLEQVVG 68
           SSLIFK + F    G LL S+   SDNDGRK YIVY+G+KPED++STP HH  MLE+VVG
Sbjct: 3   SSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVG 62

Query: 69  STFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFT 128
           STFAP+ LL+SYKRSFNGF V+L+EEEAQKI+ KEGVVSVFPNGKK++HTTRSWDFMGFT
Sbjct: 63  STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 122

Query: 129 QDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIG 188
           + V RVKQVESNI+VGVLD+GIWPESPSF+D G   P P KWKG CQ SA+F+CN+KIIG
Sbjct: 123 KSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYG-PPPAKWKGACQTSANFRCNRKIIG 182

Query: 189 ARIYRSEN-WPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIA 248
           AR YRS+N +P  DI+SPRDS+GHGTHTASTVAGGLV+QASLYGL  GTARGGVPSARIA
Sbjct: 183 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 242

Query: 249 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGIL 308
           VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+ K YFNDSIAIGAFH+MK GIL
Sbjct: 243 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 302

Query: 309 TSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQ 368
           TSNSAGN+GP +FT  N SPWSLSVAAS++DRK V++VQLG+ N +QG +INTFDL GKQ
Sbjct: 303 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 362

Query: 369 YPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASFNGTVGIV 428
           YPLI+  +APN +GGF  S SRFC  NSVD NLV GKIL+CDS+L+  T ASFNG VG+V
Sbjct: 363 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 422

Query: 429 MQSTGLKDYASSYPLPASYLRISADNDIK--LSSTAESTATILKSVAVQDASAPSVVSFS 488
           M   G+KD + SYPLP+SYL   A N+IK  + S    TATI KS AV D SAP +VSFS
Sbjct: 423 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 482

Query: 489 SRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPHATA 548
           SRGPN  T DILKPDLTAPGVEILAAWSP+A VS  V DSR+ LYNIISGTSMSCPHATA
Sbjct: 483 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 542

Query: 549 IAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLVYNAS 608
            A YVKTFHP+WSPAAIKSALMTTA  +N K+NP+AEFAYGAGHINP+KA+NPGLVY+A 
Sbjct: 543 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 602

Query: 609 ESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPSQSINQFFTR 668
           ESDY++FLCGQGYTTAMVR ++GD+S CT  N GRVWDLNYPSFALS+TPS+SINQFF R
Sbjct: 603 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 662

Query: 669 TLTNVEFGVSTYTAMVF-APPNLRITVNPPLLSFNGIGDTKSFRLTVQGTVSQAIVSASL 728
           T+TNV   V+TY A V  AP  L I+VNPP LSFN IG  KSF +TV+G V+Q IVSA+L
Sbjct: 663 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 722

Query: 729 VWSNGLHEVRSPITVYVVNKA 745
           +W++G H VRSPITVYVV+KA
Sbjct: 723 LWTDGHHHVRSPITVYVVDKA 742

BLAST of Tan0001769 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 634.8 bits (1636), Expect = 8.7e-182
Identity = 345/705 (48.94%), Postives = 456/705 (64.68%), Query Frame = 0

Query: 44  MGSKPEDTSSTPLHHRTMLEQVVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEG 103
           MG+ PE   S P HH ++L+++VG+  A   L+ SYKRSFNGFA  LS+ E+QK+   + 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 104 VVSVFPNGKKYIHTTRSWDFMGFTQDVVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDL 163
           VVSVFP+    + TTRSWDF+GF +   R    ES++IVGV+D+GIWPES SF+D G   
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFG- 120

Query: 164 PLPTKWKGKCQKSADFQCNKKIIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLV 223
           P P KWKG C+    F CN K+IGAR Y           S RD EGHGTHTAST AG  V
Sbjct: 121 PPPKKWKGSCKGGLKFACNNKLIGARFYNK------FADSARDEEGHGTHTASTAAGNAV 180

Query: 224 SQASLYGLGFGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 283
             AS YGL  GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+  +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 284 EAKSYFNDSIAIGAFHAMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQ 343
              +  N S+AIG+FHAM  GI+T+ SAGN GP   + +NVSPW ++VAAS  DR+F+ +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 344 VQLGDGNTFQGVSINTFDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGK 403
           V LG+G    G+S+NTF+L G ++P+++G+   N +   + + + +C    VD  LV GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQ---NVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 404 ILVCDSLLAAPTLASFNGTVGIVMQSTGLKDYASSYPLPASYLRISADNDIK--LSSTAE 463
           I++CD  L     A   G +G+++Q+T L D A   P PAS L       IK  + S   
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 464 STATILKSVAVQDASAPSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAV 523
             A IL++  + D  AP V SFSSRGP+ V  ++LKPD++APG+EILAA+SP+A  S  +
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 524 --EDSRSVLYNIISGTSMSCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNPE 583
             ED RSV Y+++SGTSM+CPH   +AAYVK+FHP WSP+AIKSA+MTTA  MN K NPE
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 584 AEFAYGAGHINPLKAINPGLVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGR 643
            EFAYG+G INP KA +PGLVY     DY+K LC +G+ +  +   +G N  C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 644 VWDLNYPSFALSTTPSQSINQFFTRTLTNVEFGVSTYTAMVF-APPNLRITVNPPLLSFN 703
           V DLNYP+     +     N  F RT+TNV F  STY A V    P L+I++ P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 704 GIGDTKSFRLTVQGTVSQ--AIVSASLVWSNGLHEVRSPITVYVV 742
            + + KSF +T+ G   +  + VS+S+VWS+G H VRSPI  Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Tan0001769 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 617.1 bits (1590), Expect = 1.9e-176
Identity = 347/751 (46.21%), Postives = 488/751 (64.98%), Query Frame = 0

Query: 13  FKFIFFNLLYGLLVSAGSHSDNDG-RKTYIVYMGSKPEDTSSTPL-HHRTMLEQVVGSTF 72
           F F+F +LL   L S  +  D+ G ++ YIVY+GS P     TP+  H ++L+++ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 73  APEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFTQDV 132
               L+ SYK+SFNGFA RL+E E +++A  E VVSVFP+ K  + TT SW+FMG  + +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 133 --VRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKKIIGA 192
              R + +ES+ I+GV+D+GI+PES SF+D G   P P KWKG C    +F CN K+IGA
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFG-PPPKKWKGTCAGGKNFTCNNKVIGA 186

Query: 193 RIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVY 252
           R Y +++      ++ RD  GHGTHTAS  AG  V+ ++ YGLG GTARGGVP+ARIAVY
Sbjct: 187 RDYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 253 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGILTS 312
           K+C ++GC    +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFHAM  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 313 NSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQYP 372
           N+AGN GPK  T ++ +PW  SVAAS  +R F+ +V LGDG    G S+NT+D+ G  YP
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 373 LIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDS---LLAAPTLASFNGTVGI 432
           L++G+ A  +    +   +R C+   +D  LV GKI++CDS   L+ A  L    G VG 
Sbjct: 367 LVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL----GAVGS 426

Query: 433 VMQSTGLKDYASSYPLPASYLRISADNDIK-----LSSTAESTATILKSVAVQDASAPSV 492
           ++++    D A     P S+L   +++D K     ++ST    AT+LKS  + +  AP V
Sbjct: 427 IVKNPE-PDRAFIRSFPVSFL---SNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLV 486

Query: 493 VSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCP 552
            SFSSRGP+ +  DILKPD+TAPGVEILAA+SP +  + +  D+R V Y+++SGTSM+CP
Sbjct: 487 ASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACP 546

Query: 553 HATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVN--PEAEFAYGAGHINPLKAINPG 612
           H   +AAYVKTFHP WSP+ I+SA+MTTA+ MNA  +     EFAYG+GH++P+ AINPG
Sbjct: 547 HVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPG 606

Query: 613 LVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCT---SGNIGRVWDLNYPSFALSTTPS 672
           LVY  +++D+I FLCG  YT+  +R+I+GDNS CT   S  + R  +LNYP+ +   + +
Sbjct: 607 LVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGT 666

Query: 673 QSINQFFTRTLTNVEFGVSTYTAMV--FAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGT 732
           +  N  F RT+TNV    STY A V  F    L I V+P +LS   + + +SF +TV   
Sbjct: 667 KPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSD 726

Query: 733 V--SQAIVSASLVWSNGLHEVRSPITVYVVN 743
              ++  VSA+L+WS+G H VRSPI VY ++
Sbjct: 727 SIGTKQPVSANLIWSDGTHNVRSPIIVYAMS 741

BLAST of Tan0001769 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 613.6 bits (1581), Expect = 2.1e-175
Identity = 349/744 (46.91%), Postives = 473/744 (63.58%), Query Frame = 0

Query: 8   MSSLIFKFIFFNLLYGLLVSAGSHSDNDGRKTYIVYMGSKPEDTSSTPL-HHRTMLEQVV 67
           M++L       + L  L +S+ S +  D ++ YIVYMGS       TP   H  +L++V 
Sbjct: 1   MATLAASSSLLSCLLVLFLSSVS-AVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVT 60

Query: 68  GSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGF 127
           G +     L+ SYKRSFNGFA RL+E E +++A   GVVSVFPN K  + TT SWDFMG 
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGL 120

Query: 128 TQDV--VRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCNKK 187
            + +   R   VES+ I+GV+D+GI PES SF+D G   P P KWKG C    +F CN K
Sbjct: 121 KEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFG-PPPQKWKGVCSGGKNFTCNNK 180

Query: 188 IIGARIYRSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSAR 247
           +IGAR Y SE          RD +GHGTHTAST AG  V  AS +G+G GT RGGVP++R
Sbjct: 181 LIGARDYTSE--------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASR 240

Query: 248 IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDG 307
           +A YK+C   GC    +L+AFDDAIADGVD+I++S+G   A  + ND IAIGAFHAM  G
Sbjct: 241 VAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKG 300

Query: 308 ILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLG 367
           +LT NSAGN GPK  + S V+PW L+VAAST +R FVT+V LG+G T  G S+N +++ G
Sbjct: 301 VLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKG 360

Query: 368 KQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLASFNGTVG 427
           K YPL++G+ A ++A   ++  +  C+ + VD + V GKILVC        + S  G VG
Sbjct: 361 KDYPLVYGKSAASSA--CDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVG 420

Query: 428 IVMQSTGLKDYASSYPLPASYLRISADNDI--KLSSTAESTATILKSVAVQDASAPSVVS 487
           ++ + T   D A  +PLPA+ L       +   L ST    A +LK+ A+ + ++P + S
Sbjct: 421 LIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIAS 480

Query: 488 FSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPHA 547
           FSSRGPN + +DILKPD+TAPGVEILAA+SP    S   +D+R V Y+++SGTSMSCPH 
Sbjct: 481 FSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHV 540

Query: 548 TAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVN--PEAEFAYGAGHINPLKAINPGLV 607
             +AAYVKTF+P WSP+ I+SA+MTTA+ +NA        EFAYG+GH++P+ A NPGLV
Sbjct: 541 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 600

Query: 608 YNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRVWDLNYPSFALSTTPS-QSIN 667
           Y   +SD+I FLCG  YT+ ++++I+G+   C+        +LNYPS +   + S  +  
Sbjct: 601 YELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFT 660

Query: 668 QFFTRTLTNVEFGVSTYTAMVFA--PPNLRITVNPPLLSFNGIGDTKSFRLTVQGT--VS 727
             F RTLTNV    STYT+ V A     L + + P +LSF  + + +SF +TV G+   S
Sbjct: 661 VTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDS 720

Query: 728 QAIVSASLVWSNGLHEVRSPITVY 740
           +   SA+L+WS+G H VRSPI VY
Sbjct: 721 EVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of Tan0001769 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 612.1 bits (1577), Expect = 6.0e-175
Identity = 351/752 (46.68%), Postives = 478/752 (63.56%), Query Frame = 0

Query: 8   MSSLIFKFIFFNLLYGLLV--SAGSHSDNDGRKTYIVYMGSKPEDTSSTPL-HHRTMLEQ 67
           M+     +   + ++ LLV   A +  D+  ++ YIVYMG+ P      P+ HH ++L+ 
Sbjct: 1   MTKPAVSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQD 60

Query: 68  VVGSTFAPEHLLYSYKRSFNGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFM 127
           V G +   + L+ +YKRSFNGFA RL++ E + +A  + VVSVFPN K  + TT SW+FM
Sbjct: 61  VTGESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFM 120

Query: 128 GFTQD--VVRVKQVESNIIVGVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADFQCN 187
           G  +     R   +ES+ I+GV+D+GI+PES SF+  G   P P KWKG C+   +F  N
Sbjct: 121 GLKESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFG-PPPKKWKGVCKGGKNFTWN 180

Query: 188 KKIIGARIY--RSENWPQGDIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGV 247
            K+IGAR Y  + E +P+    S RD  GHG+HTAST AG  V   S YGLG GTARGGV
Sbjct: 181 NKLIGARYYTPKLEGFPE----SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGV 240

Query: 248 PSARIAVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAF 307
           P+ARIAVYK+C    DGC    ILAAFDDAIAD VDII++S+GG+ +  +  D IAIGAF
Sbjct: 241 PAARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAF 300

Query: 308 HAMKDGILTSNSAGNEGPKFFTTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSIN 367
           HAM  GIL  NSAGN GP+  T ++++PW  +VAAS  +R FVT+V LG+G T  G S+N
Sbjct: 301 HAMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVN 360

Query: 368 TFDLLGKQYPLIHGRDAPNTAGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAAPTLAS 427
           +FDL GK+YPL++G+ A ++ G  ++    FC    +D   V GKI++CDS    P  A 
Sbjct: 361 SFDLNGKKYPLVYGKSASSSCGAASAG---FCSPGCLDSKRVKGKIVLCDS-PQNPDEAQ 420

Query: 428 FNGTVGIVMQSTGLKDYASSYPLPASYLRISADNDI--KLSSTAESTATILKSVAVQDAS 487
             G +  +++S    D AS +  P S L     N +   ++ST    A +LKS  + +  
Sbjct: 421 AMGAIASIVRS-HRTDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQR 480

Query: 488 APSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTS 547
           AP V S+ SRGPN +  DILKPD+TAPG EI+AA+SP AP S  + D+R V Y++ +GTS
Sbjct: 481 APVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTS 540

Query: 548 MSCPHATAIAAYVKTFHPTWSPAAIKSALMTTAFRMNAKVNP---EAEFAYGAGHINPLK 607
           MSCPH   +AAY+K+FHP WSP+ I+SA+MTTA+ MNA  +P    AEFAYGAGH++P+ 
Sbjct: 541 MSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPIT 600

Query: 608 AINPGLVYNASESDYIKFLCGQGYTTAMVRLITGDNSGCTSGNIGRV-WDLNYPSFALST 667
           AI+PGLVY A++SD+I FLCG  YT   +RLI+GD+S CT      +  +LNYPS     
Sbjct: 601 AIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQV 660

Query: 668 TPSQSINQFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQG 727
           + ++     F RT+TNV    +TY A V     L++ V P +LS   + + KSF +T  G
Sbjct: 661 SAAKPFKVIFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVTASG 720

Query: 728 TVSQA--IVSASLVWSNGLHEVRSPITVYVVN 743
              +A  +VSA L+WS+G+H VRSPI VY  N
Sbjct: 721 AGPKAENLVSAQLIWSDGVHFVRSPIVVYATN 738

BLAST of Tan0001769 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 610.5 bits (1573), Expect = 1.8e-174
Identity = 351/746 (47.05%), Postives = 484/746 (64.88%), Query Frame = 0

Query: 26  VSAGSHSDNDGRKTYIVYMGSKPEDT--SSTPLHHRTMLEQVVGSTFAPEHLLYSYKRSF 85
           V A +  +N  RK YIVYMG   E++   +   HH  ++  +   + A E  +YSY ++ 
Sbjct: 21  VIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKARELKIYSYGKNI 80

Query: 86  NGFAVRLSEEEAQKIALKEGVVSVFPNGKKYIHTTRSWDFMGFTQD-VVRVKQVESNIIV 145
           NGF  RL   EA+K++ +EGVVSVF N ++ +HTTRSWDF+G  +    R   +ESNIIV
Sbjct: 81  NGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIV 140

Query: 146 GVLDTGIWPESPSFNDTGLDLPLPTKWKGKCQKSADF-QCNKKIIGARIY--RSENWPQG 205
           GVLDTGI  ESPSFND G+  P P KWKGKC    +F +CN K+IGA+ +  +SE  P G
Sbjct: 141 GVLDTGIDVESPSFNDKGVG-PPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDG 200

Query: 206 DIRSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYKICWSDGCYDA 265
           +  +  D +GHGTHT+ST+AG  VS ASL+G+  GTARGGVPSARIA YK+CW  GC D 
Sbjct: 201 EGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDM 260

Query: 266 DILAAFDDAIADGVDIISLSVGGNEAKSYFNDSIAIGAFHAMKDGILTSNSAGNEGPKFF 325
           D+LAAFD+AI+DGVDIIS+S+GG  +  +F D IAIGAFHAMK GILT+ SAGN GP  F
Sbjct: 261 DMLAAFDEAISDGVDIISISIGG-ASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLF 320

Query: 326 TTSNVSPWSLSVAASTMDRKFVTQVQLGDGNTFQGVSINTFDLLGKQYPLIHGRDAPN-T 385
           T SN++PW ++VAA+++DRKF T V+LG+G T  G+S+N F+   K YPL  G  A N +
Sbjct: 321 TVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLS 380

Query: 386 AGGFNSSISRFCDENSVDLNLVSGKILVCDSLLAA---------PTLASFNGTVGIVMQS 445
           AGG+    +  C+  ++  + V GK++ C++               + S  G  G+++Q 
Sbjct: 381 AGGYGEPST--CEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKG-AGVIVQL 440

Query: 446 TGLKDYASSYPLPASYLRISADNDIK--LSSTAESTATILKSVAVQDASAPSVVSFSSRG 505
               D A+S  +  SY+       I   ++ST    A I K+   +   APS+ SFS+RG
Sbjct: 441 LEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTK-MLAPSISSFSARG 500

Query: 506 PNLVTLDILKPDLTAPGVEILAAWSPMAPVSGAVEDSRSVLYNIISGTSMSCPHATAIAA 565
           P  ++ +ILKPD++APG+ ILAA+S +A V+G  +D+R  L++I+SGTSM+CPHA A AA
Sbjct: 501 PQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPHAAAAAA 560

Query: 566 YVKTFHPTWSPAAIKSALMTTAFRMNAKVNPEAEFAYGAGHINPLKAINPGLVYNASESD 625
           YVK+FHP WSPAAIKSALMTTA  M  K N EAE +YG+G INP +AI+PGLVY+ +E  
Sbjct: 561 YVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVYDITEDA 620

Query: 626 YIKFLCGQGYTTAMVRLITGDNSGCTS------GNIGRVW---DLNYPSFALSTTPSQS- 685
           Y++FLC +GY +  + L+TGDNS  T+       NI R      LNYPS       +++ 
Sbjct: 621 YLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQVNSTEAK 680

Query: 686 INQFFTRTLTNVEFGVSTYTAMVFAPPNLRITVNPPLLSFNGIGDTKSFRLTVQGT---V 740
           +++ F RT+TNV +G STY A V+AP  LR+ V P ++SF    + ++F++ + G     
Sbjct: 681 VSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVIDGVWDET 740