Tan0001693 (gene) Snake gourd v1

Overview
NameTan0001693
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
LocationLG10: 67314381 .. 67317476 (+)
RNA-Seq ExpressionTan0001693
SyntenyTan0001693
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAACTTGGGACTGGGAGTAGATGTGGCTCTGCTTCCACAATCAACATGAAAATGGAGACTCCTCTGTTGGATGAGACAGTGGAGGGCGCAGTTGATTACAATGGCCGGCCAGTCGGCAGATCTAATTCCGGCGGATGGAGATCTGCCTCACTCATCATAGGTATAACATCATCAATCACCTTCCATTCCACTACACACTTTCTTTCTTCTGTTTCGCTATCTAACCAATCAGAATTGTGATTAGGAGTGGAGGTTGCCGAGAGATTCGCCTACTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCGGCAGTCCACCGCCACAGCAGCCGAGAATGTAAATGCCTGGTCCGGTATCGCCTCCTTGCTACCTCTACTCGGCGCCTTTCTCGCCGATTCCTTCCTCGGACGATACCGCACTATTGCTCTTTCCTCTGTTCTTTACATCCTGGTTCCTCCACTACTTTATTCTCTTCTTCTTTTACTTCTGCTTTTTTATTTCTCAACTACAAAATTTTATTGTACTATGCAAGTTATTGGCTAGGGCTCTGGTAATTCGGATAATTTCTCCCCAACTGTGTCCATATGCTTGGTCTTGAGTGAATTATTCCATCCAAGAGTCTACTCCATTTCAGTCGAGTTGAAATTTTTCTACGACTTTCACGGTTTTCATGTTGTATGCGGATAATTTCTGATTTAGCTTTAGAAAAGGCTGAATTCTGAGATCCGATGTGTCTCAATTCTGCCGTTTTTCCGAAGCTGGAAAACTTGGGAAAAGAGGTATGGGAGAGAGCTTTTGAGGACGACGAGGAGAGTTAAATATCCGCCAATATTTTATTACCCGGAATTCAAAATCAATTCTACTTTGTAATGCATGACCCATGGAATATTCTGACCTGACTACTTTTTTTTCATTTTGTTGTCTCCTTAGTTTCAACTTAGTTTTGTTTTTAGTCTGATAGATTCTTAATTTTTTATCTACCTGTCTAATAAGTTCTTGAATTTAAAAGTATCTAATCAGGCCCAAATTTCCAATTGTATATTGACATTTTTTAACATTAATAGATCTATTAGACATAAATTTGAATTTCTCTCAACCTTTATTCATGAGCTCCAAGACCATGCCTAAAGTAAACGTGTTTAACTTTTCTTTCTTTTTGTCATTTTACGTTTGGAATTCAGGGACTTGGGTTGTTGACTGTGTCTGCAACATTATCTTCTCTCAACATTTCTGCTTGCCAAGAGACTGACAAATCCATACCATGTTCCCCTAGTCTTGTCCAAGTAATTTTATTTTTCTTCTCCTTATATCTGGTGGCATTTGCTCAAGGTGGACACAGGCCTTGCGTCCAAGCTTTCGGAGCCGATCAATTTGATGGACATCATCCAGAAGAGAGCAAAGCTAAAAGCTCTTTCTTCAATTGGTGGTACTTTTGTATCTCTTTGGGCACTTTTGCAACTGTTAATGTCTTGAATTATGTGCAAGATAACCTTAGCTGGAGTTTTGGGTTTGGAATTCCTTGTATTGCTATTGCCTTTGCACTGGTAGTCTTCTTGCTTGGAACTAGGACGTACAGGTACAGTAACAGAGGAGATGAGGAAAACCCATTTGTAAGAATTGGACGGGTGTTTATCACAGCAGTAAGAAATTGGCGTGTAAATTCTCCAGAAATAGCTCATGAGGAGGAAATCCATGGCCTTTTACCTCACCATAGCTCTCAACAGTTCAGGTAAATGAGGGGACTAAAAGAAGGGTAAAATGTATGCTTTGTTGCCACTAGTCACTAGATTATATCTGTTCTTGTAAAAATTTAGTCCTTATGTGGATACAACATGTAATTGCTTTATAAACCATTTTAATTTGTCTGTATTATTATAATCCTTTTCAGAACCAGATTCTATAATTTGGCAGGTTCCTCGACAAAGCTTTGATCGTACCTAATGGTTCGAAGGAAGATGGTCATGCATGTAGTGTCAGTGAAGTTGAGGAAGCAAAAGCAGTACTAAGACTTGCTCCCATATGGGTTACTTGCTTAGCATATGCTATTGTATTCTCTCAGTTATCAACTTTCTTTACCAAACAAGGAGTCACAATGGACAGATCAATAGTTCCTGGATTTGAAATGCCTGCTGCTACACTTCAATCTTTCATGAGTATATCCACTGTCATTTCCCTCCCAATATATGATCGCATACTCATCCCAATTGCAAGAAGTTTCACTGGGAAACCTTCTGGAATTACAATGCTTCAGAGAATTGGATTTGGGATGCTTCTATCTGTGCTTTCCATGATCATTGCTGCATTAGTGGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTCGATCTACCGATGGAGACTGTTCCTTTGAGCGGATGGTGGTTGGTTCCTCAATATGCACTGGTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGACCAGGTTCCAAGTGGACTAAGAAGCATCGGTCTCTCCCTGTACCTGAGTATTTTTGGCATTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGTCATTGAAAAATTAACCAGTGGGGATGGCAAAGAAAGCTGGTTTGACAATAATCTCAACAAAGCTCATCTTGATTACTTCTATTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTCCTTTGTTTTGCAAGAACTTACATCTACAATAAGGGGAACAGCACATGAATACAACTGTTTATAAGCATTGTAATTTAAAGCTATGGGGTTGGAAATGCATTTGTTATGTATTAAAATTGAATGATTTGAAACTTGATTTCATTTTTTTAGGGAATTCCATTTTTAGAATCATCTTCAGAATTTCAGGAGGGGTAGTTGTTTAGAAGTCTTTCCTGTTTGGCCAGAACATACACCCACCATTGGAGGAATACCACATGAACTCCACCGTATTCGCTTTGTAATTTAAAGCTATGGGATTGGAGCATTGTTCTATAGAGAAATGTATTATGGTTGTACTCCTTGTCTTTTAAGTTTTATAGAGAAATGTATTATGGTTGTACTCCTTGTATTTTAAGTTTTATA

mRNA sequence

GGGAACTTGGGACTGGGAGTAGATGTGGCTCTGCTTCCACAATCAACATGAAAATGGAGACTCCTCTGTTGGATGAGACAGTGGAGGGCGCAGTTGATTACAATGGCCGGCCAGTCGGCAGATCTAATTCCGGCGGATGGAGATCTGCCTCACTCATCATAGGAGTGGAGGTTGCCGAGAGATTCGCCTACTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCGGCAGTCCACCGCCACAGCAGCCGAGAATGTAAATGCCTGGTCCGGTATCGCCTCCTTGCTACCTCTACTCGGCGCCTTTCTCGCCGATTCCTTCCTCGGACGATACCGCACTATTGCTCTTTCCTCTGTTCTTTACATCCTGGGACTTGGGTTGTTGACTGTGTCTGCAACATTATCTTCTCTCAACATTTCTGCTTGCCAAGAGACTGACAAATCCATACCATGTTCCCCTAGTCTTGTCCAAGTAATTTTATTTTTCTTCTCCTTATATCTGGTGGCATTTGCTCAAGGTGGACACAGGCCTTGCGTCCAAGCTTTCGGAGCCGATCAATTTGATGGACATCATCCAGAAGAGAGCAAAGCTAAAAGCTCTTTCTTCAATTGGTGGTACTTTTGTATCTCTTTGGGCACTTTTGCAACTGTTAATGTCTTGAATTATGTGCAAGATAACCTTAGCTGGAGTTTTGGGTTTGGAATTCCTTGTATTGCTATTGCCTTTGCACTGGTAGTCTTCTTGCTTGGAACTAGGACGTACAGGTACAGTAACAGAGGAGATGAGGAAAACCCATTTGTAAGAATTGGACGGGTGTTTATCACAGCAGTAAGAAATTGGCGTGTAAATTCTCCAGAAATAGCTCATGAGGAGGAAATCCATGGCCTTTTACCTCACCATAGCTCTCAACAGTTCAGGTTCCTCGACAAAGCTTTGATCGTACCTAATGGTTCGAAGGAAGATGGTCATGCATGTAGTGTCAGTGAAGTTGAGGAAGCAAAAGCAGTACTAAGACTTGCTCCCATATGGGTTACTTGCTTAGCATATGCTATTGTATTCTCTCAGTTATCAACTTTCTTTACCAAACAAGGAGTCACAATGGACAGATCAATAGTTCCTGGATTTGAAATGCCTGCTGCTACACTTCAATCTTTCATGAGTATATCCACTGTCATTTCCCTCCCAATATATGATCGCATACTCATCCCAATTGCAAGAAGTTTCACTGGGAAACCTTCTGGAATTACAATGCTTCAGAGAATTGGATTTGGGATGCTTCTATCTGTGCTTTCCATGATCATTGCTGCATTAGTGGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTCGATCTACCGATGGAGACTGTTCCTTTGAGCGGATGGTGGTTGGTTCCTCAATATGCACTGGTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGACCAGGTTCCAAGTGGACTAAGAAGCATCGGTCTCTCCCTGTACCTGAGTATTTTTGGCATTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGTCATTGAAAAATTAACCAGTGGGGATGGCAAAGAAAGCTGGTTTGACAATAATCTCAACAAAGCTCATCTTGATTACTTCTATTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTCCTTTGTTTTGCAAGAACTTACATCTACAATAAGGGGAACAGCACATGAATACAACTGTTTATAAGCATTGTAATTTAAAGCTATGGGGTTGGAAATGCATTTGTTATGTATTAAAATTGAATGATTTGAAACTTGATTTCATTTTTTTAGGGAATTCCATTTTTAGAATCATCTTCAGAATTTCAGGAGGGGTAGTTGTTTAGAAGTCTTTCCTGTTTGGCCAGAACATACACCCACCATTGGAGGAATACCACATGAACTCCACCGTATTCGCTTTGTAATTTAAAGCTATGGGATTGGAGCATTGTTCTATAGAGAAATGTATTATGGTTGTACTCCTTGTCTTTTAAGTTTTATAGAGAAATGTATTATGGTTGTACTCCTTGTATTTTAAGTTTTATA

Coding sequence (CDS)

ATGAAAATGGAGACTCCTCTGTTGGATGAGACAGTGGAGGGCGCAGTTGATTACAATGGCCGGCCAGTCGGCAGATCTAATTCCGGCGGATGGAGATCTGCCTCACTCATCATAGGAGTGGAGGTTGCCGAGAGATTCGCCTACTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCGGCAGTCCACCGCCACAGCAGCCGAGAATGTAAATGCCTGGTCCGGTATCGCCTCCTTGCTACCTCTACTCGGCGCCTTTCTCGCCGATTCCTTCCTCGGACGATACCGCACTATTGCTCTTTCCTCTGTTCTTTACATCCTGGGACTTGGGTTGTTGACTGTGTCTGCAACATTATCTTCTCTCAACATTTCTGCTTGCCAAGAGACTGACAAATCCATACCATGTTCCCCTAGTCTTGTCCAAGTAATTTTATTTTTCTTCTCCTTATATCTGGTGGCATTTGCTCAAGGTGGACACAGGCCTTGCGTCCAAGCTTTCGGAGCCGATCAATTTGATGGACATCATCCAGAAGAGAGCAAAGCTAAAAGCTCTTTCTTCAATTGGTGGTACTTTTGTATCTCTTTGGGCACTTTTGCAACTGTTAATGTCTTGAATTATGTGCAAGATAACCTTAGCTGGAGTTTTGGGTTTGGAATTCCTTGTATTGCTATTGCCTTTGCACTGGTAGTCTTCTTGCTTGGAACTAGGACGTACAGGTACAGTAACAGAGGAGATGAGGAAAACCCATTTGTAAGAATTGGACGGGTGTTTATCACAGCAGTAAGAAATTGGCGTGTAAATTCTCCAGAAATAGCTCATGAGGAGGAAATCCATGGCCTTTTACCTCACCATAGCTCTCAACAGTTCAGGTTCCTCGACAAAGCTTTGATCGTACCTAATGGTTCGAAGGAAGATGGTCATGCATGTAGTGTCAGTGAAGTTGAGGAAGCAAAAGCAGTACTAAGACTTGCTCCCATATGGGTTACTTGCTTAGCATATGCTATTGTATTCTCTCAGTTATCAACTTTCTTTACCAAACAAGGAGTCACAATGGACAGATCAATAGTTCCTGGATTTGAAATGCCTGCTGCTACACTTCAATCTTTCATGAGTATATCCACTGTCATTTCCCTCCCAATATATGATCGCATACTCATCCCAATTGCAAGAAGTTTCACTGGGAAACCTTCTGGAATTACAATGCTTCAGAGAATTGGATTTGGGATGCTTCTATCTGTGCTTTCCATGATCATTGCTGCATTAGTGGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTCGATCTACCGATGGAGACTGTTCCTTTGAGCGGATGGTGGTTGGTTCCTCAATATGCACTGGTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGACCAGGTTCCAAGTGGACTAAGAAGCATCGGTCTCTCCCTGTACCTGAGTATTTTTGGCATTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGTCATTGAAAAATTAACCAGTGGGGATGGCAAAGAAAGCTGGTTTGACAATAATCTCAACAAAGCTCATCTTGATTACTTCTATTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTCCTTTGTTTTGCAAGAACTTACATCTACAATAAGGGGAACAGCACATGA

Protein sequence

MKMETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLSSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEESKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYRYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIVPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPGFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMIIAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAIGLAAFLCFARTYIYNKGNST
Homology
BLAST of Tan0001693 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 4.7e-209
Identity = 372/554 (67.15%), Postives = 443/554 (79.96%), Query Frame = 0

Query: 5   TPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLR 64
           TPLL  T    VDY  +P  +S+SGGWRSA  IIGVEVAERFAYYG+SSNLI +LT  L 
Sbjct: 12  TPLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLG 71

Query: 65  QSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLSS 124
           QSTA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +S LYI+GLG+LT+SA +  
Sbjct: 72  QSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIP- 131

Query: 125 LNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEESK 184
              S C+ ++    CSP   QVI FF +LYLVA AQGGH+PCVQAFGADQFD   PEE K
Sbjct: 132 ---SDCKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECK 191

Query: 185 AKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYRY 244
           AKSSFFNWWYF +  GT  T+ VLNY+QDNLSW+ GFGIPCIA+  ALVV LLGT TYR+
Sbjct: 192 AKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRF 251

Query: 245 SNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIVPN 304
           S R ++++PFVRIG V++ AV+NW V++ ++A  EE  GL+   SSQQF FL+KAL+  N
Sbjct: 252 SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKN 311

Query: 305 GSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPGFE 364
           GS      CS+ E+EEAK+VLRLAPIW+TCL YA+VF+Q  TFFTKQG TM+RSI PG++
Sbjct: 312 GS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYK 371

Query: 365 MPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMIIAA 424
           +  ATLQSF+S+S VI +PIYDR+LIPIARSFT KP GITMLQRIG G+ LS L+M++AA
Sbjct: 372 ISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAA 431

Query: 425 LVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPSGL 484
           LVE+KRLKTA +YGLVD P  TVP+S WWLVPQY L G+ DVF MVGLQEFFYDQVP+ L
Sbjct: 432 LVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNEL 491

Query: 485 RSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSA 544
           RS+GL+LYLSIFGIGNFLSSF+IS+IEK TS  G+ SWF NNLN+AHLDYFYWLLA LS 
Sbjct: 492 RSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSF 550

Query: 545 IGLAAFLCFARTYI 559
           IGLA++L  A++Y+
Sbjct: 552 IGLASYLYVAKSYV 550

BLAST of Tan0001693 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 622.1 bits (1603), Expect = 6.2e-177
Identity = 314/560 (56.07%), Postives = 411/560 (73.39%), Query Frame = 0

Query: 4   ETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 63
           E  L +E V  AVD+ G    RSN+G WR+A  IIGVEVAERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 64  RQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLS 123
            +STA AA NVNAWSGIA+LLP+LGAF+AD+FLGRYRTI +SS++Y+LGL  LT+SA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLI 125

Query: 124 SLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEES 183
                        +  S S    +LFFFSLYLVA  Q GH+PCVQAFGADQFD    +E 
Sbjct: 126 --------PNTTEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 184 KAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYR 243
             +SSFFNWWY  +S G    + V+ Y+Q+  SW+FGFGIPC+ +  +LV+F+ G R YR
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 244 YSNRGDEE--NPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALI 303
           YS R  EE  NPF RIGRVF  A++N R++S ++   E    L  + S ++  F +KAL+
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALL 305

Query: 304 VPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVP 363
           VPN S +  +A   S+VE+A A++RL P+W T LAYAI ++Q  TFFTKQGVTMDR+I+P
Sbjct: 306 VPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILP 365

Query: 364 GFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMI 423
           G ++P A+LQ F+ IS V+ +PIYDR+ +PIAR  T +P GIT L+RIG G++LS ++M+
Sbjct: 366 GVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMV 425

Query: 424 IAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVP 483
           IAALVE KRL+TA+E+GL+D P  T+P+S WWL+PQY L+G+ADV+T+VG+QEFFY QVP
Sbjct: 426 IAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVP 485

Query: 484 SGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAG 543
           + LRSIGL+LYLS  G+G+ LSS LIS+I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAI 545

Query: 544 LSAIGLAAFLCFARTYIYNK 562
           +SA+G   FL  +++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 553

BLAST of Tan0001693 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 620.2 bits (1598), Expect = 2.4e-176
Identity = 313/560 (55.89%), Postives = 408/560 (72.86%), Query Frame = 0

Query: 4   ETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 63
           +T L +E V  AVD+ G    RS +G WR+A  IIGVEVAERFA YG+ SNLI++LT  L
Sbjct: 6   KTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPL 65

Query: 64  RQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLS 123
            QSTA AA NVNAWSGI+++LPLLGAF+AD+FLGRY TI ++S +Y+LGL  LT+SA L 
Sbjct: 66  GQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLI 125

Query: 124 SLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEES 183
             N          +  SPS     LFFFSLYLVA  Q GH+PCVQAFGADQFD  +P+E+
Sbjct: 126 PNN--------TEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQEN 185

Query: 184 KAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYR 243
             +SSFFNWWY  +  G    + V+ Y+Q+N+SW+ GFGIPC+ +  +LV+F+LG ++YR
Sbjct: 186 SDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYR 245

Query: 244 YSNRGDEE--NPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALI 303
           +S    EE  NPF RIGRVF  A +N R+NS ++   E I       S ++  FL+KAL+
Sbjct: 246 FSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALL 305

Query: 304 VPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVP 363
           VPN S E   AC   +VE+A A++RL P+W+T LAYAI F+Q  TFFTKQGVTM+R+I P
Sbjct: 306 VPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFP 365

Query: 364 GFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMI 423
           G E+P A+LQ  +SIS V+ +PIYDR+L+PI RS T  P GIT L+RIG GM+L+ L+M+
Sbjct: 366 GVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMV 425

Query: 424 IAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVP 483
           +AALVE KRL+TA+EYGL+D P  T+P+S WWL PQY L+G+ADV T+VG+QEFFY QVP
Sbjct: 426 VAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVP 485

Query: 484 SGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAG 543
           + LRS+GL++YLS  G+G+ LSS LI +I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAV 545

Query: 544 LSAIGLAAFLCFARTYIYNK 562
           +SA+G   FL  +++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 557

BLAST of Tan0001693 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 3.2e-173
Identity = 306/561 (54.55%), Postives = 410/561 (73.08%), Query Frame = 0

Query: 4   ETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 63
           E  L++++V  +VD+ G P G+S++GGWRSA  IIGVEV ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 64  RQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLS 123
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S++YILGLGLLT+S+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 124 SLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEES 183
            + +S  ++ +++    P     ILFF SLYLVA  QGGH+PCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 184 KAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYR 243
            ++ SFFNWW+  +S G   ++ V+ YVQDN++W+ GFGIPC+ +  AL +FL G +TYR
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 244 YSNRGDEE---NPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKAL 303
           Y  RGD E   N F RIGRVF+ A +N ++   ++ H  ++          Q  FL KAL
Sbjct: 247 YP-RGDREGKNNAFARIGRVFLVAFKNRKL---KLTHSGQLEVGSYKKCKGQLEFLAKAL 306

Query: 304 IVPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIV 363
           +   G  E    CS  +VE+A A++RL PIW+T +   I ++Q +TFFTKQGVT+DR I+
Sbjct: 307 LPGEGGVE---PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKIL 366

Query: 364 PGFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSM 423
           PGFE+P A+ Q+ + +S  IS+P Y+R+ +P+AR  T KPSGITMLQRIG GM+LS L+M
Sbjct: 367 PGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNM 426

Query: 424 IIAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQV 483
           ++AALVE+KRL+TA+E+GLVD P  T+P+S WW VPQY L+G+ DVF++VG QEFFYDQV
Sbjct: 427 VVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQV 486

Query: 484 PSGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLA 543
           P+ LRSIGL+L LS  G+ +FLS FLI+VI   T  +G +SWF+ NLN+AH+DYFYWLLA
Sbjct: 487 PTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLA 546

Query: 544 GLSAIGLAAFLCFARTYIYNK 562
             +AIG  AFL  +R Y+Y +
Sbjct: 547 AFTAIGFLAFLLLSRLYVYRR 560

BLAST of Tan0001693 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.1e-173
Identity = 315/558 (56.45%), Postives = 407/558 (72.94%), Query Frame = 0

Query: 9   DETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQSTA 68
           +E VE +VD+ G P  RS+SG W+S+   +  EVAE+FAY+G++SNLI + T+ L +STA
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 69  TAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLSSLNIS 128
            AA NVN W G A+ LPL+   +ADSFLGR+RTI L+S  YI+GLGLLT SAT+ SL   
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL--- 135

Query: 129 ACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEESKAKSS 188
            C + +    C  S V+VI+FF +LYL+A  +GG + C++AFGADQFD   P ESKAKSS
Sbjct: 136 -CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSS 195

Query: 189 FFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYRYSNRG 248
           +FNW YF IS+G   T  V NYVQ+NLSW+ G+ IPC+++  AL +FLLG +TYR+S  G
Sbjct: 196 YFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGG 255

Query: 249 D------EENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 308
           +       +NPFVRIGRVF+ A RN R    +         LLP+ S+++FRFLD+A+I 
Sbjct: 256 EGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCL------LLPNESTKKFRFLDRAVI- 315

Query: 309 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 368
                    +C   EVEEAKAVL L PIW+  L + IVF+Q  TFFTKQG TMDRSI   
Sbjct: 316 ---------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISST 375

Query: 369 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 428
            ++PAATLQ F+S++ ++ +PIYDR+ +PIARS T KP+GIT LQRI  G+ LS++SM+I
Sbjct: 376 LQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVI 435

Query: 429 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 488
           AALVE+KRLKTA+++GLVD P  TVP+S  WL+PQY L GV+DVFTMVGLQEFFY +VP 
Sbjct: 436 AALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPP 495

Query: 489 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 548
            LRS+GL+LYLSI GIGNFLSSF++SVIE+ TS  G+ SWF NNLN+AHLDYFYWLLA L
Sbjct: 496 QLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACL 552

Query: 549 SAIGLAAFLCFARTYIYN 561
           S++     + FA++Y+YN
Sbjct: 556 SSLAFIFTVYFAKSYLYN 552

BLAST of Tan0001693 vs. NCBI nr
Match: XP_023535811.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996.5 bits (2575), Expect = 9.3e-287
Identity = 492/563 (87.39%), Postives = 528/563 (93.78%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVEGAVDYNGRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVN WSG+ASLLPLLGAFLADSF+GRYRTIALSSVLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S +IS+CQ+T+KS+PCSP L QVILFF SLY+VAFAQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWR+NS EIAHEEEI GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           FE+PAA+LQS + +S V+SLPIYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IEKL+SGDGKESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SA+GLAAFLCFARTYIY KGN+T
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Tan0001693 vs. NCBI nr
Match: XP_023535815.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 995.3 bits (2572), Expect = 2.1e-286
Identity = 493/563 (87.57%), Postives = 525/563 (93.25%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLLDETVEGAVDYNGRPV RS  GGWRSAS IIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLDETVEGAVDYNGRPVLRSKVGGWRSASFIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVN WSG+ASLLPLLGAFLADSF+GRYRTIALSSVLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S +IS+CQ+T+KS+PCSP L QVILFF SLY+VAFAQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWR+NS EIAHEEEI GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           FE+PAA+LQS + +S V+SLPIYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IEKL+SGDGKESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SA+GLAAFLCFARTYIY KGN+T
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Tan0001693 vs. NCBI nr
Match: XP_023535812.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 994.2 bits (2569), Expect = 4.6e-286
Identity = 492/563 (87.39%), Postives = 528/563 (93.78%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVEGAVDYNGRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIALS+VLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S +IS+CQ+T+KS+PCSP L QVILFF SLY+VAFAQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWR+NS EIAHEEEI GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           FE+PAA+LQS + +S V+SLPIYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IEKL+SGDGKESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SA+GLAAFLCFARTYIY KGN+T
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Tan0001693 vs. NCBI nr
Match: XP_022973964.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 993.8 bits (2568), Expect = 6.0e-286
Identity = 495/563 (87.92%), Postives = 528/563 (93.78%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVEGAVDYNGRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIALS+VLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S  IS CQ+T+KS+PCSP L QVILFF SLY+VAFAQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWRVNS EIAHEEEI GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH CS+SEVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           F++PAA+LQSF+S+S V+SL IYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IEKL+SGDGKESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SAIGLAAFLCFARTYIY+KGN+T
Sbjct: 541 SAIGLAAFLCFARTYIYSKGNTT 563

BLAST of Tan0001693 vs. NCBI nr
Match: XP_023535809.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535810.1 protein NRT1/ PTR FAMILY 5.10-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023535814.1 protein NRT1/ PTR FAMILY 5.10-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 990.7 bits (2560), Expect = 5.1e-285
Identity = 492/563 (87.39%), Postives = 528/563 (93.78%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVEGAVDYNGRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIALS+VLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S +IS+CQ+T+KS+PCSP L QVILFF SLY+VAFAQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWRVNS EIAHEEE  GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEETRGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH C++SEVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLLPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           F++PAA+LQSF+ +S V+SLPIYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSSLSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IE L+SGDGKESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SAIGLAAFLCFARTYIY+K N+T
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNTT 563

BLAST of Tan0001693 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.9e-286
Identity = 495/563 (87.92%), Postives = 528/563 (93.78%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVEGAVDYNGRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIALS+VLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S  IS CQ+T+KS+PCSP L QVILFF SLY+VAFAQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWRVNS EIAHEEEI GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH CS+SEVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           F++PAA+LQSF+S+S V+SL IYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IEKL+SGDGKESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SAIGLAAFLCFARTYIY+KGN+T
Sbjct: 541 SAIGLAAFLCFARTYIYSKGNTT 563

BLAST of Tan0001693 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.2e-285
Identity = 492/563 (87.39%), Postives = 527/563 (93.61%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVE AVDYNGRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVNAWSG ASLLPLLGAFLADSFLGRYRTIALS+VLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S +IS CQ+T+KS+PCSP L QVILFFFSLY+VAFAQGGH+PCVQAFGADQFD  HPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWRVNS EIAHEEEI GLLPHHSSQQFRFL+KAL+V
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALLV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN SKE GH C++SEVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           F++PAA+LQSF+S+S V+SLPIYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IE L+SGDGKESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SAIGLAAFLCFARTYIY+K N+T
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNTT 563

BLAST of Tan0001693 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.8e-283
Identity = 489/563 (86.86%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLL++TVEGAVDY+GRPV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L QSTATAAENVN WS  ASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
            S +IS CQ+T+KS+PCSP L QVILFF S+Y+VAFAQGGH+PCVQAFGADQFD  HPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           SKAKSSFFNWWYFCISL TFATVN+LNYVQDNLSWS GFGIPCI++  ALVVFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+ NRGDEENPFVRIGRVFITAVRNWRVNS EIAHEEEI GLLPHHSSQQFRFL+KALIV
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFRFLNKALIV 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           P  SKE GH C++SEVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PYSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           F++PAA+LQSF+ +S V+SLPIYDRILIPIAR+FTGKPSGITMLQRIGFGMLLS LSM+I
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRL+TAQEYGLVDLP  TVPLS WWLVPQY L GVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFG+GNFLSSFLIS IE L+SGDGKESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNST 566
           SA+GLAAFLCFARTYIY KGN+T
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Tan0001693 vs. ExPASy TrEMBL
Match: A0A1S3ATI3 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.7e-276
Identity = 482/562 (85.77%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLLDETVEGAVDYNG PV RSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L+QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LSS LYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
           SS + SACQ+T+KS+PCSP+L QVILFFFSLYLV  AQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           S +KSSFFNWWYF ISL TFATVN+LNYVQDNLSWS GFGIPCIA+  ALVVF LGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+SNRGDEENPFVRIGRVFITAV+NWRVNS E+A EEEI GLLPHHSS+Q RFLDKALI 
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN  KEDG ACS+SEVEEAKAVLRL PIWVTCLA+AIVFSQ STFFTKQGVTMDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           FE+PAA+LQSF+S++ VISL IYDRILIPIAR+FTGKPSGIT LQRIGFGMLLS +SM+I
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRLKTAQEYGLVDLP  TVPLS WWLVPQY L GVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFGIGNFLSSFLIS IEKLTSG+GK+SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNS 565
           SA+GLAAFL   RTYIYNKGN+
Sbjct: 541 SALGLAAFLFSTRTYIYNKGNT 562

BLAST of Tan0001693 vs. ExPASy TrEMBL
Match: A0A5A7TJ76 (Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00110 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.7e-276
Identity = 482/562 (85.77%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 3   METPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 62
           ME PLLDETVEGAVDYNG PV RSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 63  LRQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 122
           L+QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LSS LYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 123 SSLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEE 182
           SS + SACQ+T+KS+PCSP+L QVILFFFSLYLV  AQGGH+PCVQAFGADQFDG HPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 183 SKAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTY 242
           S +KSSFFNWWYF ISL TFATVN+LNYVQDNLSWS GFGIPCIA+  ALVVF LGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 243 RYSNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 302
           R+SNRGDEENPFVRIGRVFITAV+NWRVNS E+A EEEI GLLPHHSS+Q RFLDKALI 
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 303 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 362
           PN  KEDG ACS+SEVEEAKAVLRL PIWVTCLA+AIVFSQ STFFTKQGVTMDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 363 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 422
           FE+PAA+LQSF+S++ VISL IYDRILIPIAR+FTGKPSGIT LQRIGFGMLLS +SM+I
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 423 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 482
           AALVEIKRLKTAQEYGLVDLP  TVPLS WWLVPQY L GVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 483 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 542
           GLRSIGLSLYLSIFGIGNFLSSFLIS IEKLTSG+GK+SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

Query: 543 SAIGLAAFLCFARTYIYNKGNS 565
           SA+GLAAFL   RTYIYNKGN+
Sbjct: 541 SALGLAAFLFSTRTYIYNKGNT 562

BLAST of Tan0001693 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 728.8 bits (1880), Expect = 3.4e-210
Identity = 372/554 (67.15%), Postives = 443/554 (79.96%), Query Frame = 0

Query: 5   TPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLR 64
           TPLL  T    VDY  +P  +S+SGGWRSA  IIGVEVAERFAYYG+SSNLI +LT  L 
Sbjct: 12  TPLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLG 71

Query: 65  QSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLSS 124
           QSTA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +S LYI+GLG+LT+SA +  
Sbjct: 72  QSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIP- 131

Query: 125 LNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEESK 184
              S C+ ++    CSP   QVI FF +LYLVA AQGGH+PCVQAFGADQFD   PEE K
Sbjct: 132 ---SDCKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECK 191

Query: 185 AKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYRY 244
           AKSSFFNWWYF +  GT  T+ VLNY+QDNLSW+ GFGIPCIA+  ALVV LLGT TYR+
Sbjct: 192 AKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRF 251

Query: 245 SNRGDEENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIVPN 304
           S R ++++PFVRIG V++ AV+NW V++ ++A  EE  GL+   SSQQF FL+KAL+  N
Sbjct: 252 SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKN 311

Query: 305 GSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPGFE 364
           GS      CS+ E+EEAK+VLRLAPIW+TCL YA+VF+Q  TFFTKQG TM+RSI PG++
Sbjct: 312 GS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYK 371

Query: 365 MPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMIIAA 424
           +  ATLQSF+S+S VI +PIYDR+LIPIARSFT KP GITMLQRIG G+ LS L+M++AA
Sbjct: 372 ISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAA 431

Query: 425 LVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPSGL 484
           LVE+KRLKTA +YGLVD P  TVP+S WWLVPQY L G+ DVF MVGLQEFFYDQVP+ L
Sbjct: 432 LVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNEL 491

Query: 485 RSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSA 544
           RS+GL+LYLSIFGIGNFLSSF+IS+IEK TS  G+ SWF NNLN+AHLDYFYWLLA LS 
Sbjct: 492 RSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSF 550

Query: 545 IGLAAFLCFARTYI 559
           IGLA++L  A++Y+
Sbjct: 552 IGLASYLYVAKSYV 550

BLAST of Tan0001693 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 622.1 bits (1603), Expect = 4.4e-178
Identity = 314/560 (56.07%), Postives = 411/560 (73.39%), Query Frame = 0

Query: 4   ETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 63
           E  L +E V  AVD+ G    RSN+G WR+A  IIGVEVAERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 64  RQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLS 123
            +STA AA NVNAWSGIA+LLP+LGAF+AD+FLGRYRTI +SS++Y+LGL  LT+SA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLI 125

Query: 124 SLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEES 183
                        +  S S    +LFFFSLYLVA  Q GH+PCVQAFGADQFD    +E 
Sbjct: 126 --------PNTTEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 184 KAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYR 243
             +SSFFNWWY  +S G    + V+ Y+Q+  SW+FGFGIPC+ +  +LV+F+ G R YR
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 244 YSNRGDEE--NPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALI 303
           YS R  EE  NPF RIGRVF  A++N R++S ++   E    L  + S ++  F +KAL+
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALL 305

Query: 304 VPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVP 363
           VPN S +  +A   S+VE+A A++RL P+W T LAYAI ++Q  TFFTKQGVTMDR+I+P
Sbjct: 306 VPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILP 365

Query: 364 GFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMI 423
           G ++P A+LQ F+ IS V+ +PIYDR+ +PIAR  T +P GIT L+RIG G++LS ++M+
Sbjct: 366 GVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMV 425

Query: 424 IAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVP 483
           IAALVE KRL+TA+E+GL+D P  T+P+S WWL+PQY L+G+ADV+T+VG+QEFFY QVP
Sbjct: 426 IAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVP 485

Query: 484 SGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAG 543
           + LRSIGL+LYLS  G+G+ LSS LIS+I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAI 545

Query: 544 LSAIGLAAFLCFARTYIYNK 562
           +SA+G   FL  +++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 553

BLAST of Tan0001693 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 620.2 bits (1598), Expect = 1.7e-177
Identity = 313/560 (55.89%), Postives = 408/560 (72.86%), Query Frame = 0

Query: 4   ETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 63
           +T L +E V  AVD+ G    RS +G WR+A  IIGVEVAERFA YG+ SNLI++LT  L
Sbjct: 6   KTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPL 65

Query: 64  RQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLS 123
            QSTA AA NVNAWSGI+++LPLLGAF+AD+FLGRY TI ++S +Y+LGL  LT+SA L 
Sbjct: 66  GQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLI 125

Query: 124 SLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEES 183
             N          +  SPS     LFFFSLYLVA  Q GH+PCVQAFGADQFD  +P+E+
Sbjct: 126 PNN--------TEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQEN 185

Query: 184 KAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYR 243
             +SSFFNWWY  +  G    + V+ Y+Q+N+SW+ GFGIPC+ +  +LV+F+LG ++YR
Sbjct: 186 SDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYR 245

Query: 244 YSNRGDEE--NPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALI 303
           +S    EE  NPF RIGRVF  A +N R+NS ++   E I       S ++  FL+KAL+
Sbjct: 246 FSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALL 305

Query: 304 VPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVP 363
           VPN S E   AC   +VE+A A++RL P+W+T LAYAI F+Q  TFFTKQGVTM+R+I P
Sbjct: 306 VPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFP 365

Query: 364 GFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMI 423
           G E+P A+LQ  +SIS V+ +PIYDR+L+PI RS T  P GIT L+RIG GM+L+ L+M+
Sbjct: 366 GVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMV 425

Query: 424 IAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVP 483
           +AALVE KRL+TA+EYGL+D P  T+P+S WWL PQY L+G+ADV T+VG+QEFFY QVP
Sbjct: 426 VAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVP 485

Query: 484 SGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAG 543
           + LRS+GL++YLS  G+G+ LSS LI +I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAV 545

Query: 544 LSAIGLAAFLCFARTYIYNK 562
           +SA+G   FL  +++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 557

BLAST of Tan0001693 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 609.8 bits (1571), Expect = 2.3e-174
Identity = 306/561 (54.55%), Postives = 410/561 (73.08%), Query Frame = 0

Query: 4   ETPLLDETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 63
           E  L++++V  +VD+ G P G+S++GGWRSA  IIGVEV ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 64  RQSTATAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLS 123
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S++YILGLGLLT+S+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 124 SLNISACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEES 183
            + +S  ++ +++    P     ILFF SLYLVA  QGGH+PCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 184 KAKSSFFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYR 243
            ++ SFFNWW+  +S G   ++ V+ YVQDN++W+ GFGIPC+ +  AL +FL G +TYR
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 244 YSNRGDEE---NPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKAL 303
           Y  RGD E   N F RIGRVF+ A +N ++   ++ H  ++          Q  FL KAL
Sbjct: 247 YP-RGDREGKNNAFARIGRVFLVAFKNRKL---KLTHSGQLEVGSYKKCKGQLEFLAKAL 306

Query: 304 IVPNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIV 363
           +   G  E    CS  +VE+A A++RL PIW+T +   I ++Q +TFFTKQGVT+DR I+
Sbjct: 307 LPGEGGVE---PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKIL 366

Query: 364 PGFEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSM 423
           PGFE+P A+ Q+ + +S  IS+P Y+R+ +P+AR  T KPSGITMLQRIG GM+LS L+M
Sbjct: 367 PGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNM 426

Query: 424 IIAALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQV 483
           ++AALVE+KRL+TA+E+GLVD P  T+P+S WW VPQY L+G+ DVF++VG QEFFYDQV
Sbjct: 427 VVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQV 486

Query: 484 PSGLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLA 543
           P+ LRSIGL+L LS  G+ +FLS FLI+VI   T  +G +SWF+ NLN+AH+DYFYWLLA
Sbjct: 487 PTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLA 546

Query: 544 GLSAIGLAAFLCFARTYIYNK 562
             +AIG  AFL  +R Y+Y +
Sbjct: 547 AFTAIGFLAFLLLSRLYVYRR 560

BLAST of Tan0001693 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 608.6 bits (1568), Expect = 5.1e-174
Identity = 315/558 (56.45%), Postives = 407/558 (72.94%), Query Frame = 0

Query: 9   DETVEGAVDYNGRPVGRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLRQSTA 68
           +E VE +VD+ G P  RS+SG W+S+   +  EVAE+FAY+G++SNLI + T+ L +STA
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 69  TAAENVNAWSGIASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATLSSLNIS 128
            AA NVN W G A+ LPL+   +ADSFLGR+RTI L+S  YI+GLGLLT SAT+ SL   
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL--- 135

Query: 129 ACQETDKSIPCSPSLVQVILFFFSLYLVAFAQGGHRPCVQAFGADQFDGHHPEESKAKSS 188
            C + +    C  S V+VI+FF +LYL+A  +GG + C++AFGADQFD   P ESKAKSS
Sbjct: 136 -CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSS 195

Query: 189 FFNWWYFCISLGTFATVNVLNYVQDNLSWSFGFGIPCIAIAFALVVFLLGTRTYRYSNRG 248
           +FNW YF IS+G   T  V NYVQ+NLSW+ G+ IPC+++  AL +FLLG +TYR+S  G
Sbjct: 196 YFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGG 255

Query: 249 D------EENPFVRIGRVFITAVRNWRVNSPEIAHEEEIHGLLPHHSSQQFRFLDKALIV 308
           +       +NPFVRIGRVF+ A RN R    +         LLP+ S+++FRFLD+A+I 
Sbjct: 256 EGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCL------LLPNESTKKFRFLDRAVI- 315

Query: 309 PNGSKEDGHACSVSEVEEAKAVLRLAPIWVTCLAYAIVFSQLSTFFTKQGVTMDRSIVPG 368
                    +C   EVEEAKAVL L PIW+  L + IVF+Q  TFFTKQG TMDRSI   
Sbjct: 316 ---------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISST 375

Query: 369 FEMPAATLQSFMSISTVISLPIYDRILIPIARSFTGKPSGITMLQRIGFGMLLSVLSMII 428
            ++PAATLQ F+S++ ++ +PIYDR+ +PIARS T KP+GIT LQRI  G+ LS++SM+I
Sbjct: 376 LQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVI 435

Query: 429 AALVEIKRLKTAQEYGLVDLPMETVPLSGWWLVPQYALVGVADVFTMVGLQEFFYDQVPS 488
           AALVE+KRLKTA+++GLVD P  TVP+S  WL+PQY L GV+DVFTMVGLQEFFY +VP 
Sbjct: 436 AALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPP 495

Query: 489 GLRSIGLSLYLSIFGIGNFLSSFLISVIEKLTSGDGKESWFDNNLNKAHLDYFYWLLAGL 548
            LRS+GL+LYLSI GIGNFLSSF++SVIE+ TS  G+ SWF NNLN+AHLDYFYWLLA L
Sbjct: 496 QLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACL 552

Query: 549 SAIGLAAFLCFARTYIYN 561
           S++     + FA++Y+YN
Sbjct: 556 SSLAFIFTVYFAKSYLYN 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WP014.7e-20967.15Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE26.2e-17756.07Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q0WSZ62.4e-17655.89Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9SK963.2e-17354.55Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Q9C7U17.1e-17356.45Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_023535811.19.3e-28787.39protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023535815.12.1e-28687.57protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo][more]
XP_023535812.14.6e-28687.39protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_022973964.16.0e-28687.92protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima][more]
XP_023535809.15.1e-28587.39protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
Match NameE-valueIdentityDescription
A0A6J1IG542.9e-28687.92protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 P... [more]
A0A6J1EHW23.2e-28587.39protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710... [more]
A0A6J1EC561.8e-28386.86protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731... [more]
A0A1S3ATI32.7e-27685.77protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 ... [more]
A0A5A7TJ762.7e-27685.77Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT1G22540.13.4e-21067.15Major facilitator superfamily protein [more]
AT1G72120.14.4e-17856.07Major facilitator superfamily protein [more]
AT1G72125.11.7e-17755.89Major facilitator superfamily protein [more]
AT1G22550.12.3e-17454.55Major facilitator superfamily protein [more]
AT1G72140.15.1e-17456.45Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 5..564
e-value: 6.5E-200
score: 666.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 25..555
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 99..522
e-value: 1.4E-96
score: 323.7
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 17..562
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 17..562
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 88..112
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 31..559
e-value: 0.0
score: 697.48

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001693.1Tan0001693.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity