Tan0001495 (gene) Snake gourd v1

BLAST of Tan0001495 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.2e-115
Identity = 221/490 (45.10%), Postives = 311/490 (63.47%), Query Frame = 0

Query: 14  FPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPLISLRL 73
           F  F FFIL   +   PK  LPPGP+P P++GNLLQ+G KP       +  +G L SL+L
Sbjct: 13  FLLFLFFIL---RDSRPKN-LPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSLKL 72

Query: 74  GSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHWKFLRT 133
           GSQ  V ASSPA+AA+IL+THD+ LS RY+FQ       +   + ++  EC+D+WK LR 
Sbjct: 73  GSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVE--NSIVWSECNDNWKLLRK 132

Query: 134 TCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLANVIFS 193
            CRT LFTPK +ESQS  R++    ++ +L  K G+ V   I +VV  T+ N   N+IFS
Sbjct: 133 VCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVK--IVEVVFGTLVNIFGNLIFS 192

Query: 194 KDLMEFEG--DGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARRVDS 253
           KD+ + E    G+   K+H  K++++G   N AD++PI+  LDL G ++ +AE+ +++  
Sbjct: 193 KDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYD 252

Query: 254 VYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTSTTVE 313
           V+  ++ ERR  +     G+    DF+D +L+ G  D ++  L+ E+  AGT+T+++T+E
Sbjct: 253 VWGVMLKERRGTK-----GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIE 312

Query: 314 WAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHPPAP 373
           WA+TEL K   ++ K++ E+             + ES +P L YLQA VKETLRLHPP P
Sbjct: 313 WAITELTKKPLVVSKIRLEL-----VNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTP 372

Query: 374 FLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYKGFD 433
            LLP RAL+TC VM YTIPK+ +I VN WAIGRDP  W++PL F P RFL SD+DYKG D
Sbjct: 373 LLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGND 432

Query: 434 FRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAYHKE 493
           F  +PFG GRRICPG P+     NLI+ATL++NF+WSLP     S+L +  K G+   K+
Sbjct: 433 FELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKD 484

Query: 494 EPLSLIPKNR 502
            PL ++ K R
Sbjct: 493 PPLLIVLKAR 484

BLAST of Tan0001495 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.7e-112
Identity = 208/484 (42.98%), Postives = 303/484 (62.60%), Query Frame = 0

Query: 20  FILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPLISLRLGSQLAV 79
           F+  + +   PK  LPPGPKPWP++GNLLQ+G KP       +  +G L SL+LGS+  V
Sbjct: 10  FLYLIFRDSSPK-GLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVV 69

Query: 80  FASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHWKFLRTTCRTHL 139
            AS+P +A++IL+THDR LS RY+FQ       +   + ++  EC++ WK LR  CRT L
Sbjct: 70  VASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVE--NSIVWSECNETWKKLRKVCRTEL 129

Query: 140 FTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLANVIFSKDLMEF 199
           FT K +ESQ+  R+S  + ++ YL    G+ V   I +VV  T+ N   N+IFS+++ + 
Sbjct: 130 FTQKMIESQAEVRESKAMEMVEYLKKNVGNEVK--IAEVVFGTLVNIFGNLIFSQNIFKL 189

Query: 200 --EGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARRVDSVYDDLI 259
             E  G+   K+H  +M+ELG   N AD++P L   DL G  K +A+  + + SV+  ++
Sbjct: 190 GDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAML 249

Query: 260 AERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTSTTVEWAMTEL 319
            ER+  +  +   N +  DF++ +LD G  D Q+  L+ E+  AGT+T+++T+EWA++EL
Sbjct: 250 KERKIAKQHN---NSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSEL 309

Query: 320 IKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHPPAPFLLPHR 379
            KN  +   ++ E+      +      + ES IP + YLQA VKETLRLHP  P LLP R
Sbjct: 310 TKNPQVTANMRLELLSVVGKRP-----VKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 369

Query: 380 ALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYKGFDFRYLPF 439
           AL+TC+V+ YTIPK+ +I VN W IGRDP  W +PL F P RFL S ID+KG DF  +PF
Sbjct: 370 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 429

Query: 440 GSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAYHKEEPLSLI 499
           G+GRRICPG P+    ++LI+++L++NFDW  P   DPS+L +  K G+   KE PL ++
Sbjct: 430 GAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIV 480

Query: 500 PKNR 502
           PK R
Sbjct: 490 PKTR 480

BLAST of Tan0001495 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.0e-110
Identity = 206/484 (42.56%), Postives = 302/484 (62.40%), Query Frame = 0

Query: 21  ILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPLISLRLGSQLAVF 80
           IL L+      + LPPGPKPWP++GNLLQ+G KP       +  +G + +L++G++  V 
Sbjct: 15  ILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVV 74

Query: 81  ASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHWKFLRTTCRTHLF 140
           AS+ ++A++IL+THDR LSARY+FQ       +   + ++  +C + WK LR  CRT LF
Sbjct: 75  ASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKNLRKVCRTELF 134

Query: 141 TPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLANVIFSKDLMEF- 200
           T K +ESQ+  R+     ++ YL  K G+ V   I +V+  T+ N   N+IFS+++ E  
Sbjct: 135 TQKMIESQAHVREKKCEEMVEYLMKKQGEEVK--IVEVIFGTLVNIFGNLIFSQNIFELG 194

Query: 201 -EGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARRVDSVYDDLIA 260
               G+  FK++  +M+ELG   N AD++P+L   DL G +K++AE  + + +++  ++ 
Sbjct: 195 DPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQ 254

Query: 261 ERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTSTTVEWAMTELI 320
           ER+  +  DG  +    DF+D  LD G  D Q+  L+ E+  AGT+T+++T+EWAMTEL 
Sbjct: 255 ERKLAKKVDGYKSK--NDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELT 314

Query: 321 KNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHPPAPFLLPHRA 380
           KN  I  K++ EIQ     +      + ES  P L YL+A VKETLRLHPP P LLP RA
Sbjct: 315 KNPKITAKIRSEIQTVVGERS-----VKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 374

Query: 381 LQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYKGFDFRYLPFG 440
           L+TC ++ YTIPKD +I VN W IGRDP  W +PLTF P RFL S +D++G DF  +PFG
Sbjct: 375 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFG 434

Query: 441 SGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAYHKEEPLSLIP 500
           +GRRICPG P+    + L++AT ++N DW LPN      L +  K G+   KE PL ++P
Sbjct: 435 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 487

Query: 501 KNRL 503
           K+R+
Sbjct: 495 KSRV 487

BLAST of Tan0001495 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 7.7e-110
Identity = 205/484 (42.36%), Postives = 302/484 (62.40%), Query Frame = 0

Query: 21  ILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPLISLRLGSQLAVF 80
           IL L+      + LPPGPKPWP++GNLLQ+G KP       +  +G + +L++G++  V 
Sbjct: 16  ILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVV 75

Query: 81  ASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHWKFLRTTCRTHLF 140
           AS+ ++A++IL+THDR LSARY+FQ       +   + ++  +C + WK LR  CRT LF
Sbjct: 76  ASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKNLRKVCRTELF 135

Query: 141 TPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLANVIFSKDLMEF- 200
           T K +ESQ+  R+     ++ YL  K G+ V   I +V+  T+ N   N+IFS+++ E  
Sbjct: 136 TQKMIESQAHVREKKCEEMVEYLMKKQGEEVK--IVEVIFGTLVNIFGNLIFSQNIFELG 195

Query: 201 -EGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARRVDSVYDDLIA 260
               G+  FK++  +M+ELG   N AD++P+L   DL G +K++AE  + + +++  ++ 
Sbjct: 196 XPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQ 255

Query: 261 ERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTSTTVEWAMTELI 320
           ER+  +  DG  +    DF+D  LD G  D Q+  L+ E+  AGT+T+++T+EWAMTEL 
Sbjct: 256 ERKLAKKVDGYQSK--NDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELT 315

Query: 321 KNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHPPAPFLLPHRA 380
           KN  I  K++ E+Q     +      + ES  P L YL+A VKETLRLHPP P LLP RA
Sbjct: 316 KNPKITAKLRSELQTVVGERS-----VKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 375

Query: 381 LQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYKGFDFRYLPFG 440
           L+TC ++ YTIPKD +I VN W IGRDP  W +PLTF P RFL S +D++G DF  +PFG
Sbjct: 376 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFG 435

Query: 441 SGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAYHKEEPLSLIP 500
           +GRRICPG P+    + L++AT ++N DW LPN      L +  K G+   KE PL ++P
Sbjct: 436 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 488

Query: 501 KNRL 503
           K+R+
Sbjct: 496 KSRV 488

BLAST of Tan0001495 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 6.2e-99
Identity = 207/498 (41.57%), Postives = 298/498 (59.84%), Query Frame = 0

Query: 10  LLLFFPFFFFFILKLVKSPCPK-QPLPPGPKPWPLLGNL-LQIGPKPLP---TITHFSTL 69
           L    P    FIL L     PK + LPPGP PWPL+GNL +      +P   T+ + +  
Sbjct: 7   LFSLIPVILVFILLLK----PKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMART 66

Query: 70  HGPLISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPEC 129
           HGP++ L LG+Q  V AS+  +A +IL+THDR  SAR+I       H + ++SL+ S +C
Sbjct: 67  HGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHI-KYSLVWS-DC 126

Query: 130 DDHWKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVF 189
            D+WK LR   RT +F+PK L++QS  R+  +  L+ +L SK G  V   I   V  T+ 
Sbjct: 127 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVK--ISQFVFGTLL 186

Query: 190 NTLANVIFSKDLMEF-EGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMA 249
           N L NV+FSKD+  + +     G +   ++M+ +G  PN+A+FYP L  LDLQGLKKK  
Sbjct: 187 NILGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCD 246

Query: 250 ELARRVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGT 309
           E   RV  +++  + ER+  R+++        D LD +L   F D Q+  L  E    G+
Sbjct: 247 ERFIRVMKMWEGTVKERKANRNEESK------DMLDVLLANDFNDAQINALFLETFGPGS 306

Query: 310 DTTSTTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKET 369
           +T+S T+EW + ELIK+   M KV++E+     T     + I ES +PQL YLQAC+KE 
Sbjct: 307 ETSSATIEWVIAELIKSPKEMAKVRKELNEVVGT-----STIKESDLPQLPYLQACIKEA 366

Query: 370 LRLHPPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGS 429
           +RLHP APFLLP RA +TCEVMGYTIPK++++ VN +AIGRDP  W++P TF P RFL S
Sbjct: 367 MRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLES 426

Query: 430 DIDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 489
           D+D+ G  ++++PFGSGRR C G P+    + LI+ +L+ N+D+ LP    P  L +   
Sbjct: 427 DVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEM 485

Query: 490 MGVAYHKEEPLSLIPKNR 502
           + +    +  L ++PK R
Sbjct: 487 LSLTLAIDPSLCVVPKAR 485

BLAST of Tan0001495 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 552.0 bits (1421), Expect = 5.5e-153
Identity = 285/496 (57.46%), Postives = 359/496 (72.38%), Query Frame = 0

Query: 8   PFLLLFFPFFFFFILKLVKSPCPKQ-PLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHG 67
           P LLL  P   F ILK +KSP  K+ PLPPGP PWP+LGNL QIG     +IT F+ ++G
Sbjct: 11  PSLLLLIP-PIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYG 70

Query: 68  PLISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDD 127
           PLISL+LG+Q  V ASSPA+A  +L+THDR LS RYI QM  D     + SL+ SPEC D
Sbjct: 71  PLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGD 130

Query: 128 HWKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNT 187
           HWK LR+ C+ +LFT KA+ESQ++ R+  +  L+ +L SK G  V   I+D V T+VFNT
Sbjct: 131 HWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVK--IKDFVFTSVFNT 190

Query: 188 LANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELA 247
           L+N+IFS DL++F GDG  G K  +KKMM+LGL PNLADFYP+L   DLQGL+KK A   
Sbjct: 191 LSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYM 250

Query: 248 RRVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTT 307
             VDS++  LI ERR    Q G  +    DFLD ++  GF D Q+ YLI+E+LSAGTDTT
Sbjct: 251 NEVDSLWGILIKERREIHCQQGSAS---SDFLDVLIQSGFSDQQINYLINELLSAGTDTT 310

Query: 308 STTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRL 367
           +TTVEWAM+E++K+K I++KV++EI+  T+        IDES I +L YL +CVKETLRL
Sbjct: 311 TTTVEWAMSEILKDKGILNKVRQEIKKVTKESP-----IDESNISKLQYLWSCVKETLRL 370

Query: 368 HPPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRF---LGS 427
           HPPAPFLLP  A   CEVMGY+IPKD+ IFVN+W IGRDP++WE+  TF+P RF    G+
Sbjct: 371 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 430

Query: 428 DIDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 487
           ++D+KG+D+RYLPFG GRRICPG PM ++QV LILATL+ NF+WSLPN ED SKLDL G+
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 490

Query: 488 MGVAYHKEEPLSLIPK 500
           +GV   K E L LIPK
Sbjct: 491 LGVTLQKYEALELIPK 495

BLAST of Tan0001495 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 548.9 bits (1413), Expect = 4.6e-152
Identity = 283/493 (57.40%), Postives = 350/493 (70.99%), Query Frame = 0

Query: 9   FLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPL 68
           FLLL  P   F IL+ + S   + PLPPGPKPWP+LGNLLQIG     ++T FS ++GPL
Sbjct: 8   FLLLLLP-LIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPL 67

Query: 69  ISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHW 128
           ISL+LG+Q  V ASSPA+AA IL+T DR LSARYIFQM  D       SLL SPEC D W
Sbjct: 68  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRW 127

Query: 129 KFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLA 188
           K LR+ CR  LFT +A+ESQ+  R+  +  ++ +L SK G  V   I+++V  TVFNTL+
Sbjct: 128 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVE--IKELVFITVFNTLS 187

Query: 189 NVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARR 248
           N IFS+DL+ F GDG  G K  +K M++LGL+PNLADFYP+L   DLQGL+KK A    +
Sbjct: 188 NFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 247

Query: 249 VDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTST 308
           VDS ++ LI ERR    Q   G     DFLD ++  GF D+Q+ YLI E+LSAGTDTT+T
Sbjct: 248 VDSSWEILIKERRTIHSQ---GVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTT 307

Query: 309 TVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHP 368
           TVEWAM EL+KN+ IM++V+EE +     K  N   IDES + +LHYL  CVKETLRLHP
Sbjct: 308 TVEWAMAELLKNRDIMNRVREETK-----KAINGGAIDESKLSELHYLWKCVKETLRLHP 367

Query: 369 PAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYK 428
           PAPFLLP  A + CE+MGY++PK T IFVN+W IGRDP++WE+P +F P RF G D D K
Sbjct: 368 PAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLK 427

Query: 429 GFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAY 488
           G D+R+LPFG GRRICPG PM  +QV LILATLI NF+W LPN+ED S LDL G++GV  
Sbjct: 428 GHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTL 487

Query: 489 HKEEPLSLIPKNR 502
            KEEPL L+ KNR
Sbjct: 488 QKEEPLKLLSKNR 489

BLAST of Tan0001495 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 548.5 bits (1412), Expect = 6.0e-152
Identity = 283/499 (56.71%), Postives = 357/499 (71.54%), Query Frame = 0

Query: 8   PFLLLFFPFFFFFILKLVKSPCPKQ-PLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHG 67
           P LLL  P   F ILK +KSP  K+ PLPPGP PWP+LGNL QIG     +IT F+ ++G
Sbjct: 11  PSLLLLIP-PIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYG 70

Query: 68  PLISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDD 127
           PLISL+LG+Q  V ASSPA+A  +L+THDR LS RYI QM  D     + SL+ SPEC D
Sbjct: 71  PLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGD 130

Query: 128 HWKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNT 187
           HWK LR+ C+ +LFT KA+ESQ++ R+  +  L+ +L SK G  V   I+D V T+VFNT
Sbjct: 131 HWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVK--IKDFVFTSVFNT 190

Query: 188 LANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELA 247
           L+N+IFS DL++F GDG  G K  +KKMM+LGL PNLADFYP+L   DLQGL+KK A   
Sbjct: 191 LSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYM 250

Query: 248 RRVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTT 307
             VDS++  LI ERR    Q G  +    DFLD ++  GF D Q+ YLI+E+L A TDTT
Sbjct: 251 NEVDSLWGILIKERREIHCQQGSAS---SDFLDVLIQSGFSDQQINYLINELLFAATDTT 310

Query: 308 STTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRL 367
           +TTVEWAM+E++K+K I++K +EEI    +   + +  IDES I +L YL +CVKETLRL
Sbjct: 311 TTTVEWAMSEILKDKDILNKAREEI----KKVAKESPAIDESNISKLQYLWSCVKETLRL 370

Query: 368 HPPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRF---LGS 427
           HPP PFLLP  A   CEVMGY+IPKD+ IFVN+W IGRDP++WE+  TF+P RF    G+
Sbjct: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 430

Query: 428 DIDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 487
           ++D+KG+D+RYLPFG GRRICPG PM ++QV LILATL+ NF+WSLPN ED SKLDL G+
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 490

Query: 488 MGVAYHKEEPLSLIPKNRL 503
           +GV   K E L LIPK RL
Sbjct: 491 LGVTLQKYETLKLIPKRRL 499

BLAST of Tan0001495 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 548.5 bits (1412), Expect = 6.0e-152
Identity = 287/499 (57.52%), Postives = 358/499 (71.74%), Query Frame = 0

Query: 8   PFLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGP 67
           P LLL  P   F ILK +KSP  K PLPPGP PWP+LGNLLQIG     +IT F+ ++GP
Sbjct: 11  PSLLLLLP--IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGP 70

Query: 68  LISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDH 127
           LISL+LG+Q  + ASSPA+A  +L+T DR LSARYIFQM  D     + SL+ SPEC D 
Sbjct: 71  LISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQ 130

Query: 128 WKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTL 187
           WK LR+ C+ +LFT KA+ESQ++ R+  +  L+ +L SK G  V V  +D V T+VFNTL
Sbjct: 131 WKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEV--KDFVFTSVFNTL 190

Query: 188 ANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELAR 247
           +N+IFS DL++F GDG  G K  +KKMM+LGL PNLADFYP+L   DLQGLK K      
Sbjct: 191 SNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 250

Query: 248 RVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTS 307
            VDS++  LI ERR    Q G  +    DFLD ++  GF D Q+  LI E+LSAGTDTT+
Sbjct: 251 EVDSLWGILIKERREIHCQQGSAS---NDFLDVLIQSGFDDQQINALIIELLSAGTDTTT 310

Query: 308 TTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLH 367
           TTVEWAM+E++K+K I++KV+EE+  +  TK+     IDES I +L YL  CVKETLRLH
Sbjct: 311 TTVEWAMSEILKDKDILNKVREEM--KKVTKENRP--IDESNISELQYLWRCVKETLRLH 370

Query: 368 PPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRF---LGSD 427
           PPAPFLLP  A   CEVMGY+IPKD+ +FVN+W IGRDP+VWE+  TF+P RF    G+D
Sbjct: 371 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 430

Query: 428 -IDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 487
            +D+KG+D+RYLPFG GRRICPG PM ++QV LILATLI NF+WSLPN+ED SKLDL G+
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 490

Query: 488 MGVAYHKEEPLSLIPKNRL 503
           +GV   K E L LIPK R+
Sbjct: 491 LGVTLQKYETLKLIPKRRV 498

BLAST of Tan0001495 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 548.1 bits (1411), Expect = 7.9e-152
Identity = 287/499 (57.52%), Postives = 358/499 (71.74%), Query Frame = 0

Query: 8   PFLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGP 67
           P LLL  P   F ILK +KSP  K PLPPGP PWP+LGNLLQIG     +IT F+ ++GP
Sbjct: 11  PSLLLLLP--IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGP 70

Query: 68  LISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDH 127
           LISL+LG+Q  + ASSPA+A  +L+T DR LSARYIFQM  D     + SL+ SPEC D 
Sbjct: 71  LISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQ 130

Query: 128 WKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTL 187
           WK LR+ C+ +LFT KA+ESQ++ R+  +  L+ +L SK G  V V  +D V T+VFNTL
Sbjct: 131 WKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEV--KDFVFTSVFNTL 190

Query: 188 ANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELAR 247
           +N+IFS DL++F GDG  G K  +KKMM+LGL PNLADFYP+L   DLQGLK K      
Sbjct: 191 SNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 250

Query: 248 RVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTS 307
            VDS++  LI ERR    Q G  +    DFLD ++  GF D Q+  LI E+LSAGTDTT+
Sbjct: 251 EVDSLWGILIKERREIHCQQGSAS---NDFLDVLIQSGFDDQQINALIIELLSAGTDTTT 310

Query: 308 TTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLH 367
           TTVEWAM+E++K+K I++KV+EE+  +  TK+     IDES I +L YL  CVKETLRLH
Sbjct: 311 TTVEWAMSEILKDKDILNKVREEM--KKVTKENRP--IDESNISELQYLWRCVKETLRLH 370

Query: 368 PPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRF---LGSD 427
           PPAPFLLP  A   CEVMGY+IPKD+ +FVN+W IGRDP+VWE+  TF+P RF    G+D
Sbjct: 371 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 430

Query: 428 -IDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 487
            +D+KG+D+RYLPFG GRRICPG PM ++QV LILATLI NF+WSLPN+ED SKLDL G+
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 490

Query: 488 MGVAYHKEEPLSLIPKNRL 503
           +GV   K E L LIPK R+
Sbjct: 491 LGVTLQKYETLKLIPKRRV 498

BLAST of Tan0001495 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 2.2e-152
Identity = 283/493 (57.40%), Postives = 350/493 (70.99%), Query Frame = 0

Query: 9   FLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPL 68
           FLLL  P   F IL+ + S   + PLPPGPKPWP+LGNLLQIG     ++T FS ++GPL
Sbjct: 8   FLLLLLP-LIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPL 67

Query: 69  ISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHW 128
           ISL+LG+Q  V ASSPA+AA IL+T DR LSARYIFQM  D       SLL SPEC D W
Sbjct: 68  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRW 127

Query: 129 KFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLA 188
           K LR+ CR  LFT +A+ESQ+  R+  +  ++ +L SK G  V   I+++V  TVFNTL+
Sbjct: 128 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVE--IKELVFITVFNTLS 187

Query: 189 NVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARR 248
           N IFS+DL+ F GDG  G K  +K M++LGL+PNLADFYP+L   DLQGL+KK A    +
Sbjct: 188 NFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 247

Query: 249 VDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTST 308
           VDS ++ LI ERR    Q   G     DFLD ++  GF D+Q+ YLI E+LSAGTDTT+T
Sbjct: 248 VDSSWEILIKERRTIHSQ---GVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTT 307

Query: 309 TVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHP 368
           TVEWAM EL+KN+ IM++V+EE +     K  N   IDES + +LHYL  CVKETLRLHP
Sbjct: 308 TVEWAMAELLKNRDIMNRVREETK-----KAINGGAIDESKLSELHYLWKCVKETLRLHP 367

Query: 369 PAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYK 428
           PAPFLLP  A + CE+MGY++PK T IFVN+W IGRDP++WE+P +F P RF G D D K
Sbjct: 368 PAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLK 427

Query: 429 GFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAY 488
           G D+R+LPFG GRRICPG PM  +QV LILATLI NF+W LPN+ED S LDL G++GV  
Sbjct: 428 GHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTL 487

Query: 489 HKEEPLSLIPKNR 502
            KEEPL L+ KNR
Sbjct: 488 QKEEPLKLLSKNR 489

BLAST of Tan0001495 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.9e-152
Identity = 287/499 (57.52%), Postives = 358/499 (71.74%), Query Frame = 0

Query: 8   PFLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGP 67
           P LLL  P   F ILK +KSP  K PLPPGP PWP+LGNLLQIG     +IT F+ ++GP
Sbjct: 11  PSLLLLLP--IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGP 70

Query: 68  LISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDH 127
           LISL+LG+Q  + ASSPA+A  +L+T DR LSARYIFQM  D     + SL+ SPEC D 
Sbjct: 71  LISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQ 130

Query: 128 WKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTL 187
           WK LR+ C+ +LFT KA+ESQ++ R+  +  L+ +L SK G  V V  +D V T+VFNTL
Sbjct: 131 WKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEV--KDFVFTSVFNTL 190

Query: 188 ANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELAR 247
           +N+IFS DL++F GDG  G K  +KKMM+LGL PNLADFYP+L   DLQGLK K      
Sbjct: 191 SNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 250

Query: 248 RVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTS 307
            VDS++  LI ERR    Q G  +    DFLD ++  GF D Q+  LI E+LSAGTDTT+
Sbjct: 251 EVDSLWGILIKERREIHCQQGSAS---NDFLDVLIQSGFDDQQINALIIELLSAGTDTTT 310

Query: 308 TTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLH 367
           TTVEWAM+E++K+K I++KV+EE+  +  TK+     IDES I +L YL  CVKETLRLH
Sbjct: 311 TTVEWAMSEILKDKDILNKVREEM--KKVTKENRP--IDESNISELQYLWRCVKETLRLH 370

Query: 368 PPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRF---LGSD 427
           PPAPFLLP  A   CEVMGY+IPKD+ +FVN+W IGRDP+VWE+  TF+P RF    G+D
Sbjct: 371 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 430

Query: 428 -IDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 487
            +D+KG+D+RYLPFG GRRICPG PM ++QV LILATLI NF+WSLPN+ED SKLDL G+
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 490

Query: 488 MGVAYHKEEPLSLIPKNRL 503
           +GV   K E L LIPK R+
Sbjct: 491 LGVTLQKYETLKLIPKRRV 498

BLAST of Tan0001495 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 3.8e-152
Identity = 287/499 (57.52%), Postives = 358/499 (71.74%), Query Frame = 0

Query: 8   PFLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGP 67
           P LLL  P   F ILK +KSP  K PLPPGP PWP+LGNLLQIG     +IT F+ ++GP
Sbjct: 11  PSLLLLLP--IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGP 70

Query: 68  LISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDH 127
           LISL+LG+Q  + ASSPA+A  +L+T DR LSARYIFQM  D     + SL+ SPEC D 
Sbjct: 71  LISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQ 130

Query: 128 WKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTL 187
           WK LR+ C+ +LFT KA+ESQ++ R+  +  L+ +L SK G  V V  +D V T+VFNTL
Sbjct: 131 WKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEV--KDFVFTSVFNTL 190

Query: 188 ANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELAR 247
           +N+IFS DL++F GDG  G K  +KKMM+LGL PNLADFYP+L   DLQGLK K      
Sbjct: 191 SNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 250

Query: 248 RVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTS 307
            VDS++  LI ERR    Q G  +    DFLD ++  GF D Q+  LI E+LSAGTDTT+
Sbjct: 251 EVDSLWGILIKERREIHCQQGSAS---NDFLDVLIQSGFDDQQINALIIELLSAGTDTTT 310

Query: 308 TTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLH 367
           TTVEWAM+E++K+K I++KV+EE+  +  TK+     IDES I +L YL  CVKETLRLH
Sbjct: 311 TTVEWAMSEILKDKDILNKVREEM--KKVTKENRP--IDESNISELQYLWRCVKETLRLH 370

Query: 368 PPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRF---LGSD 427
           PPAPFLLP  A   CEVMGY+IPKD+ +FVN+W IGRDP+VWE+  TF+P RF    G+D
Sbjct: 371 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 430

Query: 428 -IDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 487
            +D+KG+D+RYLPFG GRRICPG PM ++QV LILATLI NF+WSLPN+ED SKLDL G+
Sbjct: 431 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 490

Query: 488 MGVAYHKEEPLSLIPKNRL 503
           +GV   K E L LIPK R+
Sbjct: 491 LGVTLQKYETLKLIPKRRV 498

BLAST of Tan0001495 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 2.7e-150
Identity = 280/493 (56.80%), Postives = 349/493 (70.79%), Query Frame = 0

Query: 9   FLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPL 68
           FLLL  P   F IL+ + S   + PLPPGP PWP+LGNLLQIG     ++T FS  +GPL
Sbjct: 8   FLLLLLP-LIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPL 67

Query: 69  ISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHW 128
           ISL+LG+Q  V ASSPA+AA IL+T DR LSARYIFQM  D       SLL SPEC D W
Sbjct: 68  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRW 127

Query: 129 KFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLA 188
           K LR+ CR  LFT +A+ESQ+  R+  +  ++ +L SK G  V   I+++V  TVFNTL+
Sbjct: 128 KNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVE--IKELVFITVFNTLS 187

Query: 189 NVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARR 248
           N IFS+DL+ F GDG  G K  +K +++LGL+PNLADFYP+L   DLQGL+KK A    +
Sbjct: 188 NFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQ 247

Query: 249 VDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDTTST 308
           VDS ++ LI ERR    +D        DFLD +++ GF D+Q+ YLI E+LSAGTDTT+T
Sbjct: 248 VDSSWEILIKERRTIHSRDVSA---ASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTT 307

Query: 309 TVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHP 368
           TVEWAM EL+KN+ IM++V+EE +     K  N   IDES + +LHYL  CVKETLRLHP
Sbjct: 308 TVEWAMAELLKNRDIMNRVREETK-----KAINGGAIDESKLSELHYLWKCVKETLRLHP 367

Query: 369 PAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYK 428
           PAPFLLP  A + CE+MGY++PKDT IFVN+W IGRDP++WE+P +F P RF G D D K
Sbjct: 368 PAPFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLK 427

Query: 429 GFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAY 488
           G D+R+LPFG GRRICPG PM  +QV LILATLI NF+W LPN+ED S LDL G++GV  
Sbjct: 428 GHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTL 487

Query: 489 HKEEPLSLIPKNR 502
            KEEPL L+ K R
Sbjct: 488 QKEEPLKLLSKKR 489

BLAST of Tan0001495 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.4e-146
Identity = 272/497 (54.73%), Postives = 345/497 (69.42%), Query Frame = 0

Query: 6   VAPFLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLH 65
           +A F  L      F ILK + S   + P PPGPKPWP+LGNLLQIG     ++T FS ++
Sbjct: 3   IASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIY 62

Query: 66  GPLISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECD 125
           GPLISL+LG+Q  V ASSPA+AA IL+T DR LSARYIFQM  D       SL+ SPEC 
Sbjct: 63  GPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECG 122

Query: 126 DHWKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFN 185
           D WK LR+ CR  LFT +A+ESQ+  R+  +  ++ +L SK G  V   I++ V  TVFN
Sbjct: 123 DRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVE--IKEFVFITVFN 182

Query: 186 TLANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAEL 245
           TL+N IFS+DL+ F GDG  G + H+K +++  L+PNLADFYP+L   DLQGL+KK A  
Sbjct: 183 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 242

Query: 246 ARRVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDDQMRYLISEMLSAGTDT 305
             ++DS ++ LI ERR    +   G     DFLD ++  GF D+Q+ YLI E+LSAGTDT
Sbjct: 243 KNQIDSSWEILIKERRTIHSR---GVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDT 302

Query: 306 TSTTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLR 365
           T+TTVEWAM EL+KN+ IM++V+EE +     K  N   IDES + +LHYL  CVKETLR
Sbjct: 303 TTTTVEWAMAELLKNRDIMNRVREETK-----KAINGDAIDESKLSELHYLWKCVKETLR 362

Query: 366 LHPPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDI 425
           LHPPAPFLLP  A + CE+MGY++PK T I VN+W IGRDP++WE+P +F P RF   D 
Sbjct: 363 LHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDA 422

Query: 426 DYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMG 485
           D KG D+R+LPFG GRR+CPG PM  +QV LILATLI NF+W LPN+ED S LDL G++G
Sbjct: 423 DLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLG 482

Query: 486 VAYHKEEPLSLIPKNRL 503
           V   KEEPL L+PK R+
Sbjct: 483 VTLQKEEPLKLLPKRRI 489

BLAST of Tan0001495 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 336.3 bits (861), Expect = 4.4e-92
Identity = 193/505 (38.22%), Postives = 282/505 (55.84%), Query Frame = 0

Query: 9   FLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPL 68
           F+L F  F  FF     +S     P PPGP   P++GN+  +G  P  +    S  +GP+
Sbjct: 15  FVLSF--FIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPI 74

Query: 69  ISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLR-----RFSLLLSPE 128
           +SL+ GS   V  +SP +A ++LRT+D+ LS+R      T T+S+R     + S++  P 
Sbjct: 75  MSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR------TPTNSIRSINHDKVSVVWLPP 134

Query: 129 CDDHWKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTV 188
               W+ LR    T LF+P+ +E+    R++ +  L+ ++   +     V I      T 
Sbjct: 135 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITA 194

Query: 189 FNTLANVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMA 248
            N ++N++FS DL  ++ + +  F+     +ME    P+ A+F+P L  LDLQG +K + 
Sbjct: 195 LNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLK 254

Query: 249 ELARRVDSVY----DDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCDD------QMRY 308
             + R+  V+    D  +AE+        D   R  DF+D +LD+   D+       + +
Sbjct: 255 ACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRER--DFVDVLLDLTEGDEAELNTNDIVH 314

Query: 309 LISEMLSAGTDTTSTTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQL 368
           L+ ++  AGTDT S+TVEWAM EL++N   M K Q EI      K     +++ES I  L
Sbjct: 315 LLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKG----VVEESDISAL 374

Query: 369 HYLQACVKETLRLHPPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPL 428
            YLQA VKET RLHP AP L+P +A    EV+G+ +PKDT++FVN+WAIGRDP VWEN  
Sbjct: 375 PYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSS 434

Query: 429 TFDPNRFLGSDIDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNE 488
            F P RFLG DID +G D+   PFG+GRRICPG P+ V  V L+LA+L+ +FDW LPN  
Sbjct: 435 RFKPERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGV 494

Query: 489 DPSKLDLTGKMGVAYHKEEPLSLIP 499
               LD+    G+  HK  PL  +P
Sbjct: 495 GSEDLDMDETFGLTLHKTNPLHAVP 505

BLAST of Tan0001495 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 335.5 bits (859), Expect = 7.5e-92
Identity = 192/500 (38.40%), Postives = 283/500 (56.60%), Query Frame = 0

Query: 9   FLLLFFPFFFFFILKLVKSPCPKQPLPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPL 68
           F  +     FF   +  +SPC     PPGP   P++GN+  +G  P  + T  S  +GP+
Sbjct: 13  FCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPV 72

Query: 69  ISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHW 128
           +SL+LG   +V  +S  +  ++L+THD+ LS RYI + AT +++   FS+         +
Sbjct: 73  MSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISE-ATKSNNHHEFSVGWIHPSSSRF 132

Query: 129 KFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLA 188
           + LR    T LF+P+ +++    R   +  L+++L         V I  V   T  N ++
Sbjct: 133 RMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNIIS 192

Query: 189 NVIFSKDLMEFEGDGAFGFKKHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARR 248
           N++FS +L  ++   +  F++      E    P+LA+F+P +  LDLQG  KKM E + R
Sbjct: 193 NILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSGR 252

Query: 249 VDSV----YDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMGFCD------DQMRYLISEM 308
           +  V    YD  I E+   R  + D + +  DFLD ++D+   D      D++ +L+ +M
Sbjct: 253 LLQVFREFYDARIVEKSS-RSVEKDVSSK--DFLDVLIDLQQGDETEINIDEIEHLLLDM 312

Query: 309 LSAGTDTTSTTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQA 368
             AGTDT S+TVEWAM EL+ N   M KVQ+EI        QN     ES I +L YL+A
Sbjct: 313 FVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEI---NHVIGQNGD-FQESDISKLPYLKA 372

Query: 369 CVKETLRLHPPAPFLLPHRALQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPN 428
            VKET RLHP APFLL  +A    E++G+T+ KD+++ VN+WAIGRDP VWENP  F+P 
Sbjct: 373 VVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPE 432

Query: 429 RFLGSDIDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKL 488
           RFLG +ID KG D+   PFG+GRRICPG P+ +  V+L+LA+L+  F+W LPN      L
Sbjct: 433 RFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDL 492

Query: 489 DLTGKMGVAYHKEEPLSLIP 499
           D+    G+  HK  PL  +P
Sbjct: 493 DMEETFGLTVHKTNPLLAVP 504

BLAST of Tan0001495 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 332.8 bits (852), Expect = 4.8e-91
Identity = 187/480 (38.96%), Postives = 274/480 (57.08%), Query Frame = 0

Query: 34  LPPGPKPWPLLGNLLQIGPKPLPTITHFSTLHGPLISLRLGSQLAVFASSPASAAQILRT 93
           LPPGP   P++GN+ Q+G  P  +    + ++GP++SL+ G   +V  +SP +A ++LRT
Sbjct: 38  LPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRT 97

Query: 94  HDRTLSARYIFQMATDTHSLRRF-----SLLLSPECDDHWKFLRTTCRTHLFTPKALESQ 153
           HD+ LS R          S+R F     S++  P     W+ LR    T +F+P+  E+ 
Sbjct: 98  HDQILSGR------KSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEAT 157

Query: 154 SLSRKSNILALLHYLGSKTGDSVPVGIRDVVLTTVFNTLANVIFSKDLMEFEGDGAFGFK 213
              R   +  L+ ++   +     V I     TTV N ++N++FS DL  ++   +  F+
Sbjct: 158 KALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSKKSNEFQ 217

Query: 214 KHYKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARRVDSVY----DDLIAERRRYR 273
                 ME    P+ A+++P +  LDLQG +K M  L  R+  V+    D  IAE +   
Sbjct: 218 DTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAE-KSLG 277

Query: 274 DQDGDGNGRCGDFLDSMLDMGFCDD------QMRYLISEMLSAGTDTTSTTVEWAMTELI 333
           +   D + R  DFLDS+L +   D+       + +L+ +M +AGTDT+S+T+EWAM EL+
Sbjct: 278 NYSKDVSNR--DFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAELL 337

Query: 334 KNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHPPAPFLLPHRA 393
           +N   M K Q E+        QN +++ ES I  L YLQA VKET RLHP AP L+P +A
Sbjct: 338 RNPKTMVKAQAEMD---RVLGQN-SVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKA 397

Query: 394 LQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSDIDYKGFDFRYLPFG 453
               EV+G+ +PKDT++ VN+WAIGRDP+VWENP  F+P RF+G DID KG D+   PFG
Sbjct: 398 ESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDVKGRDYELTPFG 457

Query: 454 SGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAYHKEEPLSLIP 499
            GRRICPG P+ V  V+L+LA+L+ +FDW LPN      LD+    G+  H+   L  IP
Sbjct: 458 GGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAIP 504

BLAST of Tan0001495 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 328.6 bits (841), Expect = 9.1e-90
Identity = 195/506 (38.54%), Postives = 280/506 (55.34%), Query Frame = 0

Query: 4   EFVAPFLLLFFPFFFFFILKLVKSPCP-KQPLPPGPKPWPLLGNLLQIGPKPLPTITHFS 63
           + VA  L L F FF FF        CP     PPGP    LL N+LQ   KP  ++   S
Sbjct: 2   DIVAIVLSLLFIFFLFFFFYTTGKSCPGGAKNPPGPSKLSLLRNILQTVEKPHRSLADLS 61

Query: 64  TLHGPLISLRLGSQLAVFASSPASAAQILRTHDRTLSARYIFQ--MATDTHSLRRFSLLL 123
            ++G ++S +LG    V  SSP +A ++L+THD  LS R       A   H L   SLL 
Sbjct: 62  RIYGSVMSFKLGCLTTVVISSPETAKEVLKTHDHVLSYRVSSDPVRAAGHHEL---SLLW 121

Query: 124 SPECDDHWKFLRTTCRTHLFTPKALESQSLSRKSNILALLHYLGSKTGDSVPVGIRDVVL 183
            P     W+FLR   R  LF+ + LE+ S  R   +  L++++         V I     
Sbjct: 122 IPPL-ARWRFLRKITRNQLFSTQRLEATSAIRTRKVQELMNFVNKCCERREAVNISRASF 181

Query: 184 TTVFNTLANVIFSKDLMEFEGDGAF-GFKKHYKKMMELGLVPNLADFYPILSGLDLQGLK 243
            T  N ++N +FS +L  F+    F  F+    +MME+   PNLADF+P L  LDLQG +
Sbjct: 182 ITSLNIISNALFSTNLANFDDSKTFHDFQNVVIRMMEISGKPNLADFFPFLGFLDLQGAR 241

Query: 244 KKMAELARRVDSVYDDLIAERRRYRDQDGDGNGRCGDFLDSMLDMG------FCDDQMRY 303
           K+   L  ++  V+   I  +R    ++ +      D LDS+LD+         D+ +++
Sbjct: 242 KEARLLMHKLFRVFQGFIDTKRSSTSRNNN------DMLDSLLDIAHKKESELDDNNIKH 301

Query: 304 LISEMLSAGTDTTSTTVEWAMTELIKNKHIMDKVQEEIQFQTETKDQNATIIDESIIPQL 363
           L+ ++  AG DT+S+ VEWAM EL++N  ++ KVQEEI+   +      T+ D  I+ +L
Sbjct: 302 LLLDLFLAGVDTSSSAVEWAMAELLRNPKMIVKVQEEIR---QVIGLKGTVQDLDIV-KL 361

Query: 364 HYLQACVKETLRLHPPAPFLLPHRA-LQTCEVMGYTIPKDTRIFVNIWAIGRDPTVWENP 423
            YLQA VKE+LRLHPPAPFL+P ++     ++  + IPK+T++ VN+WAIGRDP VW+NP
Sbjct: 362 PYLQAVVKESLRLHPPAPFLVPRKSESDDVQIFEFLIPKNTQVLVNVWAIGRDPNVWKNP 421

Query: 424 LTFDPNRFLGSDIDYKGFDFRYLPFGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNN 483
             F+P RFLG  ID KG  F  +PFG+GRRICPG P+    ++L+LA+L+  FDW   N 
Sbjct: 422 TQFEPERFLGRGIDVKGNHFELIPFGAGRRICPGMPLAFRIMHLVLASLLYGFDWEYQNG 481

Query: 484 EDPSKLDLTGKMGVAYHKEEPLSLIP 499
             P  +D+    G   HK EPL ++P
Sbjct: 482 VVPENVDMNEAFGATLHKAEPLCIVP 493

BLAST of Tan0001495 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-89
Identity = 171/482 (35.48%), Postives = 275/482 (57.05%), Query Frame = 0

Query: 34  LPPGPKPWPLLGNLLQI-GPKPLPTITHFSTLHGPLISLRLGSQLAVFASSPASAAQILR 93
           LPPGP PWP++GN+ Q+ G  P  ++T  S  HGP+++LR+GS L V  SS   A +I +
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 94  THDRTLSARYIFQMATDTHSLRRFSLLLSPECDDHWKFLRTTCRTHLFTPKALESQSLSR 153
            HD  L+ R I++      S      L++ +   +W+ LR  C T  F  + L++ S  R
Sbjct: 96  KHDAALAGRKIYEAMKGGKS--SDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 154 KSNILALLHYL--GSKTGDSVPVGIRDVVLTTVFNTLANVIFSKDLMEFEGDGAFGFKKH 213
              +  +L ++  G + G    + +        FN + N++FS+DL++ +      F  H
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKT-IDVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFFYH 215

Query: 214 YKKMMELGLVPNLADFYPILSGLDLQGLKKKMAELARRVDSVYDDLIAERRRYRDQDGDG 273
             K+ME    PN+ADF+P+L  LD QG+++K      +   +  + I ER   R+++   
Sbjct: 216 TGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVRERE-KS 275

Query: 274 NGRCGDFLDSMLDM-----------GFCDDQMRYLISEMLSAGTDTTSTTVEWAMTELIK 333
           + +  D+LD +L+             F    +  ++ EM +AGTDTT++T+EWA+ EL+ 
Sbjct: 276 DEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLH 335

Query: 334 NKHIMDKVQEEIQFQTETKDQNATIIDESIIPQLHYLQACVKETLRLHPPAPFLLPHRAL 393
           N   + K+Q E++   ++ +Q    + E  +P L YL A + ETLRLHPP PFL+PH+A+
Sbjct: 336 NPRTLTKLQTELRTYFKSSNQK---LQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAM 395

Query: 394 QTCEVMG-YTIPKDTRIFVNIWAIGRDPTVWENPLTFDPNRFLGSD--IDYKGFDFRYLP 453
            TC +   YTIPK+T++ VN+WAIGRDP  W +P+ F P RF+      D+KG D+ +LP
Sbjct: 396 STCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLP 455

Query: 454 FGSGRRICPGYPMGVLQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGVAYHKEEPLSL 499
           FGSGRR+CP  P+    + L + +++R+FDW+L N  +  ++D+  ++G+   K  PL  
Sbjct: 456 FGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEA 510