Tan0001483 (gene) Snake gourd v1

Overview
NameTan0001483
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationLG11: 24394956 .. 24397476 (-)
RNA-Seq ExpressionTan0001483
SyntenyTan0001483
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGACTTCGTCTATAAGCAACACACAGAAACACACAAACCCACCGCCACTGCCTGTGCCTTCCACTCTGAAACAAACATAAATATAATATATAAACCCATTTCGCCATGAAAGCTTCAAAATCCTTCACTTTTGGCAGCCATGGGAAGAAAACTAGACGCTCTTCTCGGAAGGAATTTCAGAGCCTCCAAATTCCGTCCCCTTCTCAATCTCGCCCTCTCTCGCCTCGCCATCTTAACCAACCAACGCCAAGTCCGGCGCTCTCAAGCTCAATCCGACGTCCTCCAGCTCCTCCAGCTCCGCCACCACCAACGCGCTCTTCTTCGAGTCCGTTTCACTTTCTCAATCTTCCCTTCCCTTTTGCCAATTTCTGTTTTATTGTTGTTCATTTTTCTTCTTCTTCTTTTTTTTATAGGTTGAGCAAGTGATTAAGGAGCAGAATGTGTTAGATGCGTACGTTTTGATTGAAGGCTATCTCAATCTCTTGATTGAAAGGACTTCTCTCCTCGAACAGCACAGGTCCCCCTATTCTCCTTCTACCACAGATTCCTTGTTTTGTTTTGCTCTGTTTTTTTTATTTGATTTTGATTTAATCATGCAGAGAGTGCCCTGAGGAATTGAAAGAGGCGGTTGCAGGGTTGCTATTCTCTGCTTCCAGATGTGGGGATTTCCCTGAACTTCATGAGATTAAATCCGTTTTCACCTCTTGTTTTGGCAAGGAATTCACTGCTCGAGCTGTTGAATTACGCAACAACTGTGGAGTCAATCATTTGGTAATTTTTTAGAATTAAATCGATTTTTATATGATGCTCTGTTTCGTTATTCATTTTTTTGCTCTGTTTTTCTGTAGTTAATGCAAAAACTGTCCACAAGGCAGCCCAATTTGGAGAGTAGAATGGACGTGCTTAAATTCATTGCTTCTGAGAATGGAATCGCTGAGCAAATTGACGAATCTTTATCTCCTCCTCCTTCCAATTACAATCAGGTATTAGACCCAAAATCTGAAATTGATAGATCAGTCTTTGAGAAAATTCATTTTGAATGGCAATTGGGATTCTGTTTTTGGATGACTACAGGAAAAACTTGCTGGAAACACAAGACAAAACCATTCTGAGGCAGATGCTGAAACGCCGTCTGGTTCTAAACAAAAGTACAAGGATGTGGCGGATGCAGCGCAAGCGGCTTTCGAATCAGCGGCTCAAGCGGCAGCTGCTGCCAGAGCTGCCATGGAGCTCTCTCGATCTGAACCACAAGACCCTTGCTCTGGAAAGAAACCTGAGGTCGAATCTAAACCGAAACCGGAAATCAAATATGGAAATGGAAGAGAAGATGAAGAAGAAGGCAGCAAAATCAGGGCGGAAGTGAAGAATTCGATGTGTGGTTCAAGTAGAGAGTCATCTGATGAAGAAAACAGTGAAATGGATGAGCAGAGAGGTTCAAATCTTGGGAATGGGCTGGACATGGAAACGAAACTAGAGAAGGCAAGCTTTCGTTTAAATCTGGAGAAGAAGCCAATGTCAGTGAGAACAAGAAGAACGCGTGGATACTGAAGAAGAAGATCACATATGTATGTAATTATGTGGTTTAATTTGGAAATTTTGCTAGTGTAGTGTATGGAAAATTCAACATTTTTGTTTGTTTTACGTGGTATATTCAAAGTTCATGTTCAGTTATTTAATCAACCTCCCAGTCTACACCATAAACAAACAAACATGGGTTCGACTTGAAACCGGGTTCTCCTTTCTATCACACTCTTATATCATCCAATTGCGTTCTTCTTTACAAACTCTTTCCAAGAAATCTAATGAAAGCATTGATTTGTATATTCAACGAGCAAAAGATATTAATGATTGTCTGGCTAATGTTAGTATCTATATTGAGGATGAAGAGATGTTCATGAATGGTCTGCCTATTGAGTTTAGTGTTTTTCAAACTTTTATTAGAATGAGATCTGAACCTATTACCTTCGAGCATCTTTATGCTCTACTTAAAATGCGAAAACAGTCAATTGAGATGCAAATAAAGAATGTTGAAGCCTTGGTTTCAACCACTGCGTTGTTAACTCTCAACCGATCGCAAAATAACTCTAGAGGAGGGCATACTACGTGGTAATAGAGGAAACTCCAATCCGATTAGAGGACGAGGAAATTTTTCTTCTACTAGAGGTTGTTTTATCTCAAACTCTTTGAATTCTGATCCTTCTAATCATCTTGTGTGCAAAATTTGTAAGAAACCAGGATACAATGCTTTGGATTGCTTCCACCAGATGAATGATGCCTATCAAGGTTGCCATCCTCCTGAACAACTAGCTACCATGTTCCTTCTTATTCTTCTACGAATCAACCATCTAACTCTGTTTGGCTAGCAGACTCGGGATGCAATTCACATGTTACTCAGGGTGCTTCCCTTCTTGCCTCGACAGTTCCTTGCCAGAATGATGAACAAATTTCAGTTGGTGATGGTCAAGGACTTTGTTGGGGTATATGCCATAAACTCATGGTTTTTGTAAATTCTTGATGAA

mRNA sequence

GGAGACTTCGTCTATAAGCAACACACAGAAACACACAAACCCACCGCCACTGCCTGTGCCTTCCACTCTGAAACAAACATAAATATAATATATAAACCCATTTCGCCATGAAAGCTTCAAAATCCTTCACTTTTGGCAGCCATGGGAAGAAAACTAGACGCTCTTCTCGGAAGGAATTTCAGAGCCTCCAAATTCCGTCCCCTTCTCAATCTCGCCCTCTCTCGCCTCGCCATCTTAACCAACCAACGCCAAGTCCGGCGCTCTCAAGCTCAATCCGACGTCCTCCAGCTCCTCCAGCTCCGCCACCACCAACGCGCTCTTCTTCGAGTTGAGCAAGTGATTAAGGAGCAGAATGTGTTAGATGCGTACGTTTTGATTGAAGGCTATCTCAATCTCTTGATTGAAAGGACTTCTCTCCTCGAACAGCACAGAGAGTGCCCTGAGGAATTGAAAGAGGCGGTTGCAGGGTTGCTATTCTCTGCTTCCAGATGTGGGGATTTCCCTGAACTTCATGAGATTAAATCCGTTTTCACCTCTTGTTTTGGCAAGGAATTCACTGCTCGAGCTGTTGAATTACGCAACAACTGTGGAGTCAATCATTTGTTAATGCAAAAACTGTCCACAAGGCAGCCCAATTTGGAGAGTAGAATGGACGTGCTTAAATTCATTGCTTCTGAGAATGGAATCGCTGAGCAAATTGACGAATCTTTATCTCCTCCTCCTTCCAATTACAATCAGGAAAAACTTGCTGGAAACACAAGACAAAACCATTCTGAGGCAGATGCTGAAACGCCGTCTGGTTCTAAACAAAAGTACAAGGATGTGGCGGATGCAGCGCAAGCGGCTTTCGAATCAGCGGCTCAAGCGGCAGCTGCTGCCAGAGCTGCCATGGAGCTCTCTCGATCTGAACCACAAGACCCTTGCTCTGGAAAGAAACCTGAGGTCGAATCTAAACCGAAACCGGAAATCAAATATGGAAATGGAAGAGAAGATGAAGAAGAAGGCAGCAAAATCAGGGCGGAAGTGAAGAATTCGATGTGTGGTTCAAGTAGAGAGTCATCTGATGAAGAAAACAGTGAAATGGATGAGCAGAGAGGTTCAAATCTTGGGAATGGGCTGGACATGGAAACGAAACTAGAGAAGGCAAGCTTTCGTTTAAATCTGGAGAAGAAGCCAATGTCAGTGAGAACAAGAAGAACGCGTGGATACTGAAGAAGAAGATCACATATGTATGTAATTATGTGGTTTAATTTGGAAATTTTGCTAGTGTAGTGTATGGAAAATTCAACATTTTTGTTTGTTTTACGTGGTATATTCAAAGTTCATGTTCAGTTATTTAATCAACCTCCCAGTCTACACCATAAACAAACAAACATGGGTTCGACTTGAAACCGGGTTCTCCTTTCTATCACACTCTTATATCATCCAATTGCGTTCTTCTTTACAAACTCTTTCCAAGAAATCTAATGAAAGCATTGATTTGTATATTCAACGAGCAAAAGATATTAATGATTGTCTGGCTAATGTTAGTATCTATATTGAGGATGAAGAGATGTTCATGAATGGTCTGCCTATTGAGTTTAGTGTTTTTCAAACTTTTATTAGAATGAGATCTGAACCTATTACCTTCGAGCATCTTTATGCTCTACTTAAAATGCGAAAACAGTCAATTGAGATGCAAATAAAGAATGTTGAAGCCTTGGTTTCAACCACTGCGTTGTTAACTCTCAACCGATCGCAAAATAACTCTAGAGGAGGGCATACTACGTGGTAATAGAGGAAACTCCAATCCGATTAGAGGACGAGGAAATTTTTCTTCTACTAGAGGTTGTTTTATCTCAAACTCTTTGAATTCTGATCCTTCTAATCATCTTGTGTGCAAAATTTGTAAGAAACCAGGATACAATGCTTTGGATTGCTTCCACCAGATGAATGATGCCTATCAAGGTTGCCATCCTCCTGAACAACTAGCTACCATGTTCCTTCTTATTCTTCTACGAATCAACCATCTAACTCTGTTTGGCTAGCAGACTCGGGATGCAATTCACATGTTACTCAGGGTGCTTCCCTTCTTGCCTCGACAGTTCCTTGCCAGAATGATGAACAAATTTCAGTTGGTGATGGTCAAGGACTTTGTTGGGGTATATGCCATAAACTCATGGTTTTTGTAAATTCTTGATGAA

Coding sequence (CDS)

ATGGGAAGAAAACTAGACGCTCTTCTCGGAAGGAATTTCAGAGCCTCCAAATTCCGTCCCCTTCTCAATCTCGCCCTCTCTCGCCTCGCCATCTTAACCAACCAACGCCAAGTCCGGCGCTCTCAAGCTCAATCCGACGTCCTCCAGCTCCTCCAGCTCCGCCACCACCAACGCGCTCTTCTTCGAGTTGAGCAAGTGATTAAGGAGCAGAATGTGTTAGATGCGTACGTTTTGATTGAAGGCTATCTCAATCTCTTGATTGAAAGGACTTCTCTCCTCGAACAGCACAGAGAGTGCCCTGAGGAATTGAAAGAGGCGGTTGCAGGGTTGCTATTCTCTGCTTCCAGATGTGGGGATTTCCCTGAACTTCATGAGATTAAATCCGTTTTCACCTCTTGTTTTGGCAAGGAATTCACTGCTCGAGCTGTTGAATTACGCAACAACTGTGGAGTCAATCATTTGTTAATGCAAAAACTGTCCACAAGGCAGCCCAATTTGGAGAGTAGAATGGACGTGCTTAAATTCATTGCTTCTGAGAATGGAATCGCTGAGCAAATTGACGAATCTTTATCTCCTCCTCCTTCCAATTACAATCAGGAAAAACTTGCTGGAAACACAAGACAAAACCATTCTGAGGCAGATGCTGAAACGCCGTCTGGTTCTAAACAAAAGTACAAGGATGTGGCGGATGCAGCGCAAGCGGCTTTCGAATCAGCGGCTCAAGCGGCAGCTGCTGCCAGAGCTGCCATGGAGCTCTCTCGATCTGAACCACAAGACCCTTGCTCTGGAAAGAAACCTGAGGTCGAATCTAAACCGAAACCGGAAATCAAATATGGAAATGGAAGAGAAGATGAAGAAGAAGGCAGCAAAATCAGGGCGGAAGTGAAGAATTCGATGTGTGGTTCAAGTAGAGAGTCATCTGATGAAGAAAACAGTGAAATGGATGAGCAGAGAGGTTCAAATCTTGGGAATGGGCTGGACATGGAAACGAAACTAGAGAAGGCAAGCTTTCGTTTAAATCTGGAGAAGAAGCCAATGTCAGTGAGAACAAGAAGAACGCGTGGATACTGA

Protein sequence

MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASENGIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEADAETPSGSKQKYKDVADAAQAAFESAAQAAAAARAAMELSRSEPQDPCSGKKPEVESKPKPEIKYGNGREDEEEGSKIRAEVKNSMCGSSRESSDEENSEMDEQRGSNLGNGLDMETKLEKASFRLNLEKKPMSVRTRRTRGY
Homology
BLAST of Tan0001483 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.4e-11
Identity = 54/189 (28.57%), Postives = 98/189 (51.85%), Query Frame = 0

Query: 10  GRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVIKE 69
           G ++ + K +  L LA+SR+ IL N++       + +V +LL+ ++ + A +RVE +I++
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELHEIKSV 129
           + +++ + +IE    LL  R +L+    E P E+KE++  L++S+ R    PEL +IK+ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 FTSCFGKEFTARAVELRNNC----GVNHLLMQKLSTRQPNLESRMDVLKFIASENGIAEQ 189
             + +GK      +E   NC     VN  ++ KLS   P+       L  IA +  + + 
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNV-DW 184

Query: 190 IDESLSPPP 195
                 PPP
Sbjct: 185 CGSDYPPPP 186

BLAST of Tan0001483 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.4e-10
Identity = 45/158 (28.48%), Postives = 82/158 (51.90%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A+ ++   L     +RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRC-GDFPELHEI 127
           +E  +++A  ++E Y +LL+ R  L++  +E    L E+V+ L+++A R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
                + + KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Tan0001483 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.4e-10
Identity = 45/158 (28.48%), Postives = 82/158 (51.90%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A+ ++   L     +RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRC-GDFPELHEI 127
           +E  +++A  ++E Y +LL+ R  L++  +E    L E+V+ L+++A R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
                + + KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Tan0001483 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.4e-10
Identity = 45/158 (28.48%), Postives = 82/158 (51.90%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A+ ++   L     +RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRC-GDFPELHEI 127
           +E  +++A  ++E Y +LL+ R  L++  +E    L E+V+ L+++A R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
                + + KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Tan0001483 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.4e-09
Identity = 48/159 (30.19%), Postives = 81/159 (50.94%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A+ ++   L     +RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELHEIK 127
           +E  +++A  ++E Y +LL+ R  L++  +E    L E+V+ L+++A R     E+ E+K
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQS--EVAELK 120

Query: 128 SVFTSCFGKEFTARAVELRNN--CGVNHLLMQKLSTRQP 165
            V      K         R N    VN  LM KLS   P
Sbjct: 121 IVADQLCAKYSKGYGKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Tan0001483 vs. NCBI nr
Match: XP_022921602.1 (uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 465.3 bits (1196), Expect = 4.7e-127
Identity = 288/393 (73.28%), Postives = 307/393 (78.12%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNLALSRLAILTNQR++RRSQAQSDVLQLLQL H QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVLIEGYLNLLIERTSLLEQ R+CPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV TS FGKEFTARAVELRNNCGVNHLLMQKLSTR PNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEA----------DAETPSGSKQKYKDVAD 240
           GI  Q+DE+   P SN      A N+RQN SE             E PSGSKQKYKDVAD
Sbjct: 181 GITLQLDET---PLSNE-----ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVAD 240

Query: 241 AAQAAFESAAQAAAAARAAMELSRSEPQDP-----------------CSGKKPEVESKPK 300
           AAQAAFESAAQAAAAARAAMELSRSE QDP                    KK EVE+KPK
Sbjct: 241 AAQAAFESAAQAAAAARAAMELSRSESQDPDGPSSPGPGSATTSYHKQEQKKSEVEAKPK 300

Query: 301 PEIKYGNGREDEEEGSKIR--AEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDME 357
            EI+Y  G+  EEEGSKIR  AEVKNSMCG  S+RE   +EN EMDEQR +N+  G +ME
Sbjct: 301 SEIEYEYGK--EEEGSKIRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEME 360

BLAST of Tan0001483 vs. NCBI nr
Match: XP_023538782.1 (uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 463.8 bits (1192), Expect = 1.4e-126
Identity = 288/386 (74.61%), Postives = 308/386 (79.79%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNLALSRLAILTNQR++RRSQAQSDVLQLLQLRH QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLRHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVLIEGYLNLLIERTSLLEQ RECPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV TS FGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDE---SLSPPPSNYNQEKLAGNTRQNHSEADAETPSGSKQKYKDVADAAQAAFE 240
           GI  Q+DE   S     S+ NQ +  G   +N  +   E PSGSKQKYKDVADAAQAAFE
Sbjct: 181 GITLQLDETPLSNEARNSSQNQPEPHGVVGEN-LKLSTEVPSGSKQKYKDVADAAQAAFE 240

Query: 241 SAAQAAAAARAAMELSRSEPQD--------PCSG---------KKPEVESKPKPEIKYGN 300
           SAAQAAAAARAAMELSR E QD        P SG         KK +VE+KPK EI+Y  
Sbjct: 241 SAAQAAAAARAAMELSRPESQDPDGPSSPGPGSGTTSYDKQEQKKSDVEAKPKLEIEYEY 300

Query: 301 GREDEEEGSKIR--AEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDMETKLEKA- 357
           GR  EEEGSKIR  AEVKNSMCG  S+RE   +EN EMDEQR SN+  G +ME  LEK  
Sbjct: 301 GR--EEEGSKIRAEAEVKNSMCGCSSNRELFVKENREMDEQRASNIEIGEEMEANLEKTE 360

BLAST of Tan0001483 vs. NCBI nr
Match: KAG6601794.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 462.6 bits (1189), Expect = 3.0e-126
Identity = 287/393 (73.03%), Postives = 306/393 (77.86%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNLA+SRLAILTNQR++RRSQAQSDVLQLLQL H QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVLIEGYLNLLIER SLLEQ RECPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV TS FGKEFTARAVELRNNCGVNHLLMQKLSTR PNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEA----------DAETPSGSKQKYKDVAD 240
           GI  Q+DE+   P SN      A N+RQN SE             E PSGSKQKYKDVAD
Sbjct: 181 GITLQLDET---PLSNE-----ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVAD 240

Query: 241 AAQAAFESAAQAAAAARAAMELSRSEPQDP-----------------CSGKKPEVESKPK 300
           AAQAAFESAAQAAAAARAAMELSRSE QDP                    KK EVE+KPK
Sbjct: 241 AAQAAFESAAQAAAAARAAMELSRSESQDPDGPSSPGPGSATTSYHKQEQKKSEVEAKPK 300

Query: 301 PEIKYGNGREDEEEGSKIR--AEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDME 357
            EI+Y  G+  EEEGSKIR  AEVKNSMCG  S+RE   +EN EMDEQR +N+  G +ME
Sbjct: 301 SEIEYEYGK--EEEGSKIRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEME 360

BLAST of Tan0001483 vs. NCBI nr
Match: XP_022971762.1 (uncharacterized protein LOC111470447 [Cucurbita maxima])

HSP 1 Score: 461.5 bits (1186), Expect = 6.8e-126
Identity = 286/393 (72.77%), Postives = 306/393 (77.86%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNLA+SRLAILTNQR++RR+QAQSDVLQLLQL H QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVL+EGYLNLLIERTSLLEQ RECPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV T+ FGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSVLTTHFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSE----------ADAETPSGSKQKYKDVAD 240
           GI  Q+DE+   P SN      A N+RQN SE             E PSGSKQKYKDVAD
Sbjct: 181 GITLQLDET---PLSNE-----ARNSRQNQSEPHGGVGENLKLSTEVPSGSKQKYKDVAD 240

Query: 241 AAQAAFESAAQAAAAARAAMELSRSEPQDP-----------------CSGKKPEVESKPK 300
           AAQAAFESAAQAAAAARAAMELSRSE QDP                    KK  VE+KPK
Sbjct: 241 AAQAAFESAAQAAAAARAAMELSRSESQDPDGPSSPGPGSATSSYDKQEQKKSNVEAKPK 300

Query: 301 PEIKYGNGREDEEEGSKIR--AEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDME 357
            EI+Y  GR  EEEGSKIR   EVKNSMCG  S+RE   +EN EMDEQR SN+  G +ME
Sbjct: 301 LEIEYEYGR--EEEGSKIRPKPEVKNSMCGCSSNRELFVKENREMDEQRASNIEIGEEME 360

BLAST of Tan0001483 vs. NCBI nr
Match: XP_022139373.1 (uncharacterized protein LOC111010325 [Momordica charantia])

HSP 1 Score: 449.5 bits (1155), Expect = 2.7e-122
Identity = 279/394 (70.81%), Postives = 303/394 (76.90%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGRKLDALLGRNFRASKFRPLLNLALSRLA+LTNQR VRRSQA+SD LQLLQL HH RAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIKEQN LDAYVLIEGYLNLLIERT LLEQ RECPEELKEAV+GL+F+ASRCGDF
Sbjct: 61  LRVEQVIKEQNALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PEL EIKSV T+ FGKEFTARAVELRNNCGV+H +MQKLSTRQPNLESRM+VL+ IASEN
Sbjct: 121 PELQEIKSVLTTRFGKEFTARAVELRNNCGVDHSIMQKLSTRQPNLESRMNVLEAIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEA-----------DAETPSGS---KQKYK 240
            I  Q+DE    P S  N+EK A N RQN+ +             A+ PSGS   KQKYK
Sbjct: 181 NIVLQLDEH---PLS--NEEKFARNRRQNYQQEPKAEVGDNLQFSADVPSGSMQAKQKYK 240

Query: 241 DVADAAQAAFESAAQAAAAARAAMELSRSEPQD--------PCSG--------KKPEVES 300
           DVADAAQAAFESAAQAAAAARAAMELSRS+ QD        P SG        KK EVES
Sbjct: 241 DVADAAQAAFESAAQAAAAARAAMELSRSQSQDPDDPSTPKPGSGSTNKRQEQKKSEVES 300

Query: 301 KPKPEIKYGNGREDEEEGSKIRAEVKNSMCGSSRESSDEENSEMDEQRGSNLGNGLDMET 357
           KPK EI+Y N RE+EE GSKI AEVKNSM  S   S   E+SEM+EQR  N+  GLDMET
Sbjct: 301 KPKQEIEYQNRREEEEAGSKITAEVKNSMPASF--SPSREDSEMEEQRAPNVETGLDMET 360

BLAST of Tan0001483 vs. ExPASy TrEMBL
Match: A0A6J1E0Z0 (uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC111429814 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.3e-127
Identity = 288/393 (73.28%), Postives = 307/393 (78.12%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNLALSRLAILTNQR++RRSQAQSDVLQLLQL H QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVLIEGYLNLLIERTSLLEQ R+CPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV TS FGKEFTARAVELRNNCGVNHLLMQKLSTR PNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEA----------DAETPSGSKQKYKDVAD 240
           GI  Q+DE+   P SN      A N+RQN SE             E PSGSKQKYKDVAD
Sbjct: 181 GITLQLDET---PLSNE-----ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVAD 240

Query: 241 AAQAAFESAAQAAAAARAAMELSRSEPQDP-----------------CSGKKPEVESKPK 300
           AAQAAFESAAQAAAAARAAMELSRSE QDP                    KK EVE+KPK
Sbjct: 241 AAQAAFESAAQAAAAARAAMELSRSESQDPDGPSSPGPGSATTSYHKQEQKKSEVEAKPK 300

Query: 301 PEIKYGNGREDEEEGSKIR--AEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDME 357
            EI+Y  G+  EEEGSKIR  AEVKNSMCG  S+RE   +EN EMDEQR +N+  G +ME
Sbjct: 301 SEIEYEYGK--EEEGSKIRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEME 360

BLAST of Tan0001483 vs. ExPASy TrEMBL
Match: A0A6J1I2V3 (uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 3.3e-126
Identity = 286/393 (72.77%), Postives = 306/393 (77.86%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNLA+SRLAILTNQR++RR+QAQSDVLQLLQL H QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVL+EGYLNLLIERTSLLEQ RECPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV T+ FGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSVLTTHFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSE----------ADAETPSGSKQKYKDVAD 240
           GI  Q+DE+   P SN      A N+RQN SE             E PSGSKQKYKDVAD
Sbjct: 181 GITLQLDET---PLSNE-----ARNSRQNQSEPHGGVGENLKLSTEVPSGSKQKYKDVAD 240

Query: 241 AAQAAFESAAQAAAAARAAMELSRSEPQDP-----------------CSGKKPEVESKPK 300
           AAQAAFESAAQAAAAARAAMELSRSE QDP                    KK  VE+KPK
Sbjct: 241 AAQAAFESAAQAAAAARAAMELSRSESQDPDGPSSPGPGSATSSYDKQEQKKSNVEAKPK 300

Query: 301 PEIKYGNGREDEEEGSKIR--AEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDME 357
            EI+Y  GR  EEEGSKIR   EVKNSMCG  S+RE   +EN EMDEQR SN+  G +ME
Sbjct: 301 LEIEYEYGR--EEEGSKIRPKPEVKNSMCGCSSNRELFVKENREMDEQRASNIEIGEEME 360

BLAST of Tan0001483 vs. ExPASy TrEMBL
Match: A0A6J1CFG6 (uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010325 PE=3 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.3e-122
Identity = 279/394 (70.81%), Postives = 303/394 (76.90%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGRKLDALLGRNFRASKFRPLLNLALSRLA+LTNQR VRRSQA+SD LQLLQL HH RAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIKEQN LDAYVLIEGYLNLLIERT LLEQ RECPEELKEAV+GL+F+ASRCGDF
Sbjct: 61  LRVEQVIKEQNALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PEL EIKSV T+ FGKEFTARAVELRNNCGV+H +MQKLSTRQPNLESRM+VL+ IASEN
Sbjct: 121 PELQEIKSVLTTRFGKEFTARAVELRNNCGVDHSIMQKLSTRQPNLESRMNVLEAIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEA-----------DAETPSGS---KQKYK 240
            I  Q+DE    P S  N+EK A N RQN+ +             A+ PSGS   KQKYK
Sbjct: 181 NIVLQLDEH---PLS--NEEKFARNRRQNYQQEPKAEVGDNLQFSADVPSGSMQAKQKYK 240

Query: 241 DVADAAQAAFESAAQAAAAARAAMELSRSEPQD--------PCSG--------KKPEVES 300
           DVADAAQAAFESAAQAAAAARAAMELSRS+ QD        P SG        KK EVES
Sbjct: 241 DVADAAQAAFESAAQAAAAARAAMELSRSQSQDPDDPSTPKPGSGSTNKRQEQKKSEVES 300

Query: 301 KPKPEIKYGNGREDEEEGSKIRAEVKNSMCGSSRESSDEENSEMDEQRGSNLGNGLDMET 357
           KPK EI+Y N RE+EE GSKI AEVKNSM  S   S   E+SEM+EQR  N+  GLDMET
Sbjct: 301 KPKQEIEYQNRREEEEAGSKITAEVKNSMPASF--SPSREDSEMEEQRAPNVETGLDMET 360

BLAST of Tan0001483 vs. ExPASy TrEMBL
Match: A0A6J1E4E5 (uncharacterized protein LOC111429823 OS=Cucurbita moschata OX=3662 GN=LOC111429823 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.9e-122
Identity = 279/393 (70.99%), Postives = 299/393 (76.08%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGR LDALLGRNFRASKFRPLLNL LSRLAILTNQR++RRSQAQSDVLQLLQL H QRAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLTLSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVEQVIK+QN LDAYVLIEGYLNLLIER SLLEQ RECPEELKEA+AGLLF+ASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKS  TS FGKEFTARAVELRNNCGVNHLLMQKLSTR PNLESRMD+LK IASEN
Sbjct: 121 PELHEIKSALTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEAD----------AETPSGSKQKYKDVAD 240
           GI  Q+DE+   P SN      A N+RQN  E D           E PSGSKQKYKDVAD
Sbjct: 181 GITLQLDET---PLSNE-----ARNSRQNQPETDGGVGENLKFSTEVPSGSKQKYKDVAD 240

Query: 241 AAQAAFESAAQAAAAARAAMELSRSEPQDPCSGKKP-----------------EVESKPK 300
           AAQAAFESAAQAAAAARAAMELSR E QDP     P                 +VE+KPK
Sbjct: 241 AAQAAFESAAQAAAAARAAMELSRPESQDPDGPSSPGPGSATTSYHKQVQKESDVEAKPK 300

Query: 301 PEIKYGNGREDEEEGSKI--RAEVKNSMCG--SSRESSDEENSEMDEQRGSNLGNGLDME 357
            EI+Y  GR  EEEGSK+  + EVKNSMCG  S+RE   +EN EMDEQR +N+  G +ME
Sbjct: 301 LEIEYEYGR--EEEGSKVTPKPEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEME 360

BLAST of Tan0001483 vs. ExPASy TrEMBL
Match: A0A0A0KAU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 5.3e-108
Identity = 258/382 (67.54%), Postives = 282/382 (73.82%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MGRKLDALLGRNFRASKFRPLLNL+LSRL+ILT QR+V  SQA SDVLQLLQL HH RAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
           LRVE+VIK+QN LDAYVLIEGYLNLL+ERT+LLEQ  ECPEELKEAVAGLLF+ASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PELHEIKSV T+ FGKEFTARAVELRNNCGVN  LMQKLSTRQP LE+RMD LK IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEADA-----ETPSGSKQKYKDVADAAQAA 240
           GI  QID+     PS+  QEK+  N RQ+ +E  +     E  SGSK  YKDVADAAQAA
Sbjct: 181 GIVLQIDQL----PSS-KQEKVGRNGRQSEAEGQSEEFSNEVASGSKTTYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSR-----SEPQDPCSG---------KKPEVESKPKPEI-KYGN 300
           FESAAQAAAAARAAMELSR     S P  P SG         +K EVESK K E+ +YGN
Sbjct: 241 FESAAQAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGN 300

Query: 301 GREDEEEGSKIRAEVKNSMCGSSRESSDEENSEMDEQRGSNLGNGLDMETKL------EK 357
           GR+ E EG                   +EE   MDE+R S   NGL METK+      EK
Sbjct: 301 GRKGEGEG------------------EEEEGDGMDEERTS---NGLKMETKVEKTEVSEK 356

BLAST of Tan0001483 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.4e-62
Identity = 176/409 (43.03%), Postives = 234/409 (57.21%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRAL 60
           MG+KLDALLGR+F+ +KF+ L+ LAL+RL+IL NQRQ R SQA SDV +LL+L  H+ A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  LRVEQVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDF 120
            RV+QV+K+QN LD    I GY  L ++R  L E +R+CPEEL EAV+GLLF+ASR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELHEIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 180
           PEL EI++V  S FGK+  AR++ELR+NCGV+  ++QKLSTR P  E RM  LK IA+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GIAEQIDESLSPPPSNYNQE--------KLAGNTRQNHSEADAETPSGSKQKYKDVADAA 240
            I  ++D++ +      N +        KL     +      +++    K+KYKDVADAA
Sbjct: 181 NIVLKLDQASTSTEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRGKKKYKDVADAA 240

Query: 241 QAAFESAAQAAAAARAAMELSRSEPQDPCS-----GKKPEVESKPKPEIKYGNGREDEEE 300
           QAAFESAA AA AA+AA+ELS+  P+   S     G+     S+ K   +   G +D  E
Sbjct: 241 QAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHGSENKESEQEQEGNDDSSE 300

Query: 301 G-SKIRAEVKNSMCGSS----------RE-------------SSDEE----------NSE 357
           G   + +E K SM  S           RE              S+EE          +  
Sbjct: 301 GEGDVMSESKRSMSDSEDIIDVPVMSFREDPVKLLEKDTIIYDSEEETQPSVETITISKS 360

BLAST of Tan0001483 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 129.0 bits (323), Expect = 7.4e-30
Identity = 64/176 (36.36%), Postives = 113/176 (64.20%), Query Frame = 0

Query: 5   LDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVE 64
           LD+   + F+A+K + LL L + R+ ++ N+R+ +  Q + ++ +LL+      A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  QVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELH 124
            +I+E+ ++ A  ++E +  L+  R  ++E  RECP +LKEA++ + F+A RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASEN 181
           +++ +F S +GKEF A A EL+ + GVN  L++ LS R P+ E+++ +LK IA E+
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH 179

BLAST of Tan0001483 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-29
Identity = 70/189 (37.04%), Postives = 116/189 (61.38%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQVI 67
           +L R F+ +K +  L +A SRL IL N+++++  Q + ++ QLL+      A +RVE V+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 68  KEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELHEIK 127
           +E+  + AY LI  Y  LL+ R  ++E  + CP +LKEAV  +LF++ R  D PEL EI 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 128 SVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASENGIAEQID 187
             FT+ +GK+F+  AVELR + GV+ LL++KLS + P+  +++ +L  IA E+ +  +  
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQ 183

Query: 188 ESLSPPPSN 197
             +   P +
Sbjct: 184 SFVESDPKD 192

BLAST of Tan0001483 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 123.2 bits (308), Expect = 4.1e-28
Identity = 83/255 (32.55%), Postives = 138/255 (54.12%), Query Frame = 0

Query: 5   LDALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVE 64
           L+ L  R    +K +  LNLA++R+ +L N+R ++    + ++   LQ      A +RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  QVIKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELH 124
            VI+E N+  AY ++E +   ++ R  +LE  +ECP EL+EA+A ++F+A RC + P+L 
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIKSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIASENGIAE 184
           +IK++F + +GKEF   A ELR + GVN  +++KLS   P+  +R+ +LK IA E  +  
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSL-- 183

Query: 185 QIDESLSPPPSNYNQEKLAGNTRQNHSEADAETPSGSKQKY--KDVADAAQAAFESAAQA 244
             D S +      + E L G  +Q H +        S+Q Y    V+   ++    A Q 
Sbjct: 184 NWDSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQR 243

Query: 245 AAAARAAMELSRSEP 258
               +A   +S+S P
Sbjct: 244 FQKLQAQNPVSKSMP 256

BLAST of Tan0001483 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 119.0 bits (297), Expect = 7.7e-27
Identity = 98/333 (29.43%), Postives = 161/333 (48.35%), Query Frame = 0

Query: 7   ALLGRNFRASKFRPLLNLALSRLAILTNQRQVRRSQAQSDVLQLLQLRHHQRALLRVEQV 66
           +L  R F +SK +    +A++R+ ++ N+R V   Q + D+  LLQ      A +RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKEQNVLDAYVLIEGYLNLLIERTSLLEQHRECPEELKEAVAGLLFSASRCGDFPELHEI 126
           I+EQN+  A  +IE +  L++ R +++ + ++CP +LKE +A L+F+A RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 KSVFTSCFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDVLKFIA--------- 186
           + +F   +GK+F + A +LR +CGVN +L+ KLS R P  E ++ ++K IA         
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 187 --SENGIAEQIDESLSPPPSNYNQEKLAGNTRQNHSEAD-------AETPSGSKQKYKDV 246
             +E  + +  +ES+  P    +   L  N    +   D       + +       Y D 
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDT 259

Query: 247 ADAAQAAFESAAQAAAAARAAMELSRSEPQDPCSGKKPEVESKPKPEIKYGNGREDEEEG 306
             AA+AA E A QA AAA+ A  L+        S K+  V S      K     +     
Sbjct: 260 ESAAEAATELAKQAVAAAQVASLLATRRDS---SNKEFSVSSDHSTHQKDSQYMDHHHHH 319

Query: 307 SKIRAEVKNSMCGSSRESSDEENSEMDEQRGSN 322
              R + ++S   S       EN  M  +   N
Sbjct: 320 PGSRRQSRDSETSSYYAKPGAENRGMGRRHSYN 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I394.4e-1128.57IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
P539906.4e-1028.48IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX006.4e-1028.48IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z66.4e-1028.48IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Q5R6G81.4e-0930.19IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022921602.14.7e-12773.28uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-lik... [more]
XP_023538782.11.4e-12674.61uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo][more]
KAG6601794.13.0e-12673.03IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022971762.16.8e-12672.77uncharacterized protein LOC111470447 [Cucurbita maxima][more]
XP_022139373.12.7e-12270.81uncharacterized protein LOC111010325 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1E0Z02.3e-12773.28uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC1114298... [more]
A0A6J1I2V33.3e-12672.77uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447... [more]
A0A6J1CFG61.3e-12270.81uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1E4E52.9e-12270.99uncharacterized protein LOC111429823 OS=Cucurbita moschata OX=3662 GN=LOC1114298... [more]
A0A0A0KAU05.3e-10867.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G13340.17.4e-6243.03Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.27.4e-3036.36Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.11.3e-2937.04Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.14.1e-2832.55Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.17.7e-2729.43Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 21..182
e-value: 6.5E-47
score: 159.5
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 8..314
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..190
e-value: 1.9E-58
score: 199.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..330
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 258..293
NoneNo IPR availablePANTHERPTHR12161:SF16REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 8..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001483.1Tan0001483.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport