Tan0001458 (gene) Snake gourd v1

Overview
NameTan0001458
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description5-formyltetrahydrofolate cyclo-ligase-like protein COG0212
LocationLG11: 8901284 .. 8905359 (-)
RNA-Seq ExpressionTan0001458
SyntenyTan0001458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTATCTCATCGTCCTCCGTGGCGATCTCTCCACGACCACTCACAGTTATCAGTTTCGCCCCAAACTGAAATTCTCGTTCATGGATTCCAGTCTTCTGCAACTATCCCACCCTCTATCAGTCAAATCCACATTTCTCAACGCAATTCGGAAATCTCGTTCTATTTCGTCAATCTCCATCGGAAGAAATTTTCAGTTCCAAAGGCACTTCAAGCTGGAGAGTAGTAGAGACAACAGCAGCAAAACCCAACACGGTATCGCTTTCGACGAAGCTGCTTTTGAGGCCGAGAGGTCTCGCCTCGACGCCCAAGCTCAAAAATCCATGGCCGAAGCTTCAATCAGGGACATGGAAGGCGCTGCCGACGACGACCCGAAAGCTTGGAAGTGGGTGATCCGAAAACGAATTTGGGATTTGATGGAATCGAAAAACATTGCCGCAAATCCCCGACCCGTTCACCATCGCATCCCTAATTTCGTTGGCGCCATGGAAGCTGTCAATAGAGTGAGCACTCCAATTCCTGAATTGTTTGAATCAATATATTGGAAACTTTGATTCCTTCCGCATATACCTGATGAAATGGAGTTCTATCAGTTGTGCGATTTGGAAGTGTTTAGGGATTCTCAGTGCGTGAAGGTTAATCCGGATTCCCCTCAGAAGGGTGTGAGGCTGCTTACACTTACGGGTCAGTTCTGCTGACACTTTCTATTTTTTATTCCCTCGACGTATTAGTTTGGGCTACTTTTTTGTTCTTGTTGGTTTGTTTTAGTTTTTTAGATCGTCTGCCATATACTATGTCGTCAAGAGACAATGTTTCAAGTTGGCAATGAATTCGAACACTCACTACTAGTACAATTTAAATATGATTTAGATTGGCGATTTTGATGTCATCTCAATCAGACAAACATGGTTTCCATCTTAAAAATAATTGACAAAGAAAGGAGTAACTAATGTAAGATTGTGAAGCATCTTAAATCTTTCTGATGTGGGTGATCCCCCACACCCTCGCCTACCCAAGGCCCAGATTCGAGAGATATAAGGTTTTTATTTATTAAACTCACCCGAGGGCTCAAATTTATTACAAGAGAACCAAGCACTTACTTACGGGGTTGTCTCTCGAGGGATTCTAAATTTTTGTTATTAAAACACTTTTGTTATATGATGCTCTGTATATGCATGATTCATCATTCGTGTACTAATATCTGCAACAATATGATAAACGATTAATAAATAAGCGATCGAGAGGATTTTTTGGTTATTCTGGATAACCCTGTAGTATTTATCAAGAACAGAGTGTAAGGTATCTTCTTACAATATATTGATATTCAAGAGAAAATCGGTTATACAAGCAAAGAAAAACCCAGTACAAAAGAGATTACTCTCCCTCCCCCAAGCCGTAGGCTTCTTCTCCCCCCAAAAACATCCCTACCCCCTCTTGCAAGAAAACCTTCCCTCATAACCCTCGCGGTGGTCCCTCCTTTCTTACACAATTTCCAAAATACCCGGCCGTAACTAATCACTGTTCTACCCCTTTACTTCTCCTATGATAAACTTTCATAACAGGTGGACTCTTGACACAGAGTTCACTCAAGAATGTTAGAACCTTATAAAGCACTTCAACGTCAACTGGTCATGTCTTAGTTTATTACAAAAACTAAGAAAAATCTAGTGAAATTATGAATTTCTAATAGTAAACTTGATGGTTGGCTGAGTAAAAAATGTTGTCAGATTTAATCATCATTGTCAGGTAGTTGAATCAATTCGGTGTAGGTTTCGACTGAATCATGAACTTCATTGTAATGACTTAATTGAGTTGTTTAAATAAAACTTGCATACTTTCATTTAAACCTTGCAGGCTTATAAATAGCCATTTGTACATGAGAATAACAGCTGTAATAATGAGAGTATAAGAAACTAAAACAAACTAACTAATGGAATAACTAGTTTGTTATCTGTAATAGTATTTGATTCCCTATAAGCATGATAGCCTTCTTCATGTTGAATCACAAACCAAATGGTATTTGATTATGATCAGGTGGCAAAAAACTATTAACTCCTCAGCCCCGATTAAGAACAGGGTTTTTCTCCATAGTCGAATCTGGAATGTTGACTCCTGCTACCATCAACGAGGCCTGCACTTCAGTTGGGGTAGCAAAGTATGGAAAGCCAATTGGACTTGATGAAAAGATCAAAGTTGATCTGATCGTCATTGGCTCTGTTGCTGTTGACCCCAGAACAGGTGCACGGCTTGGCAAAGGAGAGGTAAGAGTGTTTCTGCTGGCTTTATCTTTTATATTGAAAATGGCTGGTTGTAAAGCATGTTTGCTCCGAAAGATTTTATAATCCTCGGTCTTGAGTTATCTGTTTGGAATGACTCTGTTCATATTTTCTCTTATCCAAAAGGTAACCAACAGCATGAAGGATAAAACTTAAAAGTGCGATGTTAGCACATAACTTACATCTGTAGTTCTGTTCTTGCTTCCTCTGCCACTTTGAAAATACAATGTATTGATTAATTTTTGTATTATTATGCGAGAGAAAGAGCATGCTTGATATATCTATGTTTCTTTATGAAACCGAAGCTTCTGTTTTGATTAAAAGAAAGCATATGATAGAACCCAAATGTATTGCAATATACAAGATAATGGAACTAACAAGATAAAACAAGTAATTTGAGGTATTTAGATCCTCCCTATCTTGAGATCGCTCAAGCCTTAAACCAACTAAATTAATGCATGACTTTCTCTGTTCCCACCCCCTCTAATTATAGCTAAACAAATACCATAACAACCTCCCTAACTAATTACTAATATACTCTTATAGTATCCCTAATTCCTTCTAATGACATTGCTATCACAATACAAATGAAATGCAGGGATTTGCAGAACTCGAATATGGAATGCTGCGGTACATGGGAGCCATTGATGACTCAACTCCAATTGTTACTTCTGGTTGTGTGACTACTTATTTTCTGGCTTCTCTCTCTTTTCATTATTTTCAATAACAAGATGTTGTGTTACATATTTGAACCGGTGGAGATAAATTTCAGTGCCAAATTATCTACATTACTACACATCCTCTACTAATCAACTTAGATTGTGCAATTTTAGCAGTTGATTTCCCGAAATATATAGTTTCCACGCCTCCCCCATTCTCAAGAAAAAGAAAATACTTCTTTTACAAATTTAAGTACAAAGATGATTATCAGTCAAGATTAAACTTTTGTCAAGCAGTTGAGATAATTCATGGGCAGAGGGCTGTCAGTTTAAGAATGATGGTGATAACTGATAACACTAACTTATTGATATGTTTTAAGATACAAATTGATAGCTTACATATCATTGAGCCTCCCTGTTTGTTCATACATGTTAAAGCTCACATTTTCTTTGCTCTTTTGGCTGTACATTTTCATGAACTTCATGTCCTAATATGGACTTCCAATTCCCTTGCAGTGCACGATTGCCAATTGGTTGATGATATTCCAGTTGAGAAGCTATTAGTCCACGACGTGCCAGTAGACATCGTATGCACTCCGACTCAGGTCATTTTAACCAACACGAAAATCCCCAAACCCCAAGGTTAGCTCTGCATTTTCATGTCACCCTCCAGAAAAACAAGTTTGACCCCTCCTGATGCCTGCATCTATATTCAATATTTGTAGCAGTGAACTGATTTGTTATCAACAAGAGAGTAATCAATGTTTTGAATTGTAGGCATTTACTGGGAAATGTTGTCTCCTGAGAAGCTGAGTCAAGTTCGAATACTCAGGGAGCTGAAACGTCGGATCGAACGGGAAATCGGCCAACCACTGCCATGTGGTCCATCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGTAAAACGTGCATCCTCCAAGAACTGATCAAAGGGGAAACAGAGAAAGAAACACAAACCTGTTTTATATTTTCCTTCTTTAGATAGTTTTGGTGTTGAATAAAAACTCTCCTCCAAGACAGACGAGGAAGATTTTAAAGGAACAAACAAATGAAAAAATAAGGTTAAATTACAAGTTTAATCCCTAAATTTTTATGTTTATATCTATTTGGTC

mRNA sequence

GTTTTATCTCATCGTCCTCCGTGGCGATCTCTCCACGACCACTCACAGTTATCAGTTTCGCCCCAAACTGAAATTCTCGTTCATGGATTCCAGTCTTCTGCAACTATCCCACCCTCTATCAGTCAAATCCACATTTCTCAACGCAATTCGGAAATCTCGTTCTATTTCGTCAATCTCCATCGGAAGAAATTTTCAGTTCCAAAGGCACTTCAAGCTGGAGAGTAGTAGAGACAACAGCAGCAAAACCCAACACGGTATCGCTTTCGACGAAGCTGCTTTTGAGGCCGAGAGGTCTCGCCTCGACGCCCAAGCTCAAAAATCCATGGCCGAAGCTTCAATCAGGGACATGGAAGGCGCTGCCGACGACGACCCGAAAGCTTGGAAGTGGGTGATCCGAAAACGAATTTGGGATTTGATGGAATCGAAAAACATTGCCGCAAATCCCCGACCCGTTCACCATCGCATCCCTAATTTCGTTGGCGCCATGGAAGCTGTCAATAGATTGTGCGATTTGGAAGTGTTTAGGGATTCTCAGTGCGTGAAGGTTAATCCGGATTCCCCTCAGAAGGGTGTGAGGCTGCTTACACTTACGGGTGGCAAAAAACTATTAACTCCTCAGCCCCGATTAAGAACAGGGTTTTTCTCCATAGTCGAATCTGGAATGTTGACTCCTGCTACCATCAACGAGGCCTGCACTTCAGTTGGGGTAGCAAAGTATGGAAAGCCAATTGGACTTGATGAAAAGATCAAAGTTGATCTGATCGTCATTGGCTCTGTTGCTGTTGACCCCAGAACAGGTGCACGGCTTGGCAAAGGAGAGGGATTTGCAGAACTCGAATATGGAATGCTGCGGTACATGGGAGCCATTGATGACTCAACTCCAATTGTTACTTCTGTGCACGATTGCCAATTGGTTGATGATATTCCAGTTGAGAAGCTATTAGTCCACGACGTGCCAGTAGACATCGTATGCACTCCGACTCAGGTCATTTTAACCAACACGAAAATCCCCAAACCCCAAGGCATTTACTGGGAAATGTTGTCTCCTGAGAAGCTGAGTCAAGTTCGAATACTCAGGGAGCTGAAACGTCGGATCGAACGGGAAATCGGCCAACCACTGCCATGTGGTCCATCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGTAAAACGTGCATCCTCCAAGAACTGATCAAAGGGGAAACAGAGAAAGAAACACAAACCTGTTTTATATTTTCCTTCTTTAGATAGTTTTGGTGTTGAATAAAAACTCTCCTCCAAGACAGACGAGGAAGATTTTAAAGGAACAAACAAATGAAAAAATAAGGTTAAATTACAAGTTTAATCCCTAAATTTTTATGTTTATATCTATTTGGTC

Coding sequence (CDS)

ATGGATTCCAGTCTTCTGCAACTATCCCACCCTCTATCAGTCAAATCCACATTTCTCAACGCAATTCGGAAATCTCGTTCTATTTCGTCAATCTCCATCGGAAGAAATTTTCAGTTCCAAAGGCACTTCAAGCTGGAGAGTAGTAGAGACAACAGCAGCAAAACCCAACACGGTATCGCTTTCGACGAAGCTGCTTTTGAGGCCGAGAGGTCTCGCCTCGACGCCCAAGCTCAAAAATCCATGGCCGAAGCTTCAATCAGGGACATGGAAGGCGCTGCCGACGACGACCCGAAAGCTTGGAAGTGGGTGATCCGAAAACGAATTTGGGATTTGATGGAATCGAAAAACATTGCCGCAAATCCCCGACCCGTTCACCATCGCATCCCTAATTTCGTTGGCGCCATGGAAGCTGTCAATAGATTGTGCGATTTGGAAGTGTTTAGGGATTCTCAGTGCGTGAAGGTTAATCCGGATTCCCCTCAGAAGGGTGTGAGGCTGCTTACACTTACGGGTGGCAAAAAACTATTAACTCCTCAGCCCCGATTAAGAACAGGGTTTTTCTCCATAGTCGAATCTGGAATGTTGACTCCTGCTACCATCAACGAGGCCTGCACTTCAGTTGGGGTAGCAAAGTATGGAAAGCCAATTGGACTTGATGAAAAGATCAAAGTTGATCTGATCGTCATTGGCTCTGTTGCTGTTGACCCCAGAACAGGTGCACGGCTTGGCAAAGGAGAGGGATTTGCAGAACTCGAATATGGAATGCTGCGGTACATGGGAGCCATTGATGACTCAACTCCAATTGTTACTTCTGTGCACGATTGCCAATTGGTTGATGATATTCCAGTTGAGAAGCTATTAGTCCACGACGTGCCAGTAGACATCGTATGCACTCCGACTCAGGTCATTTTAACCAACACGAAAATCCCCAAACCCCAAGGCATTTACTGGGAAATGTTGTCTCCTGAGAAGCTGAGTCAAGTTCGAATACTCAGGGAGCTGAAACGTCGGATCGAACGGGAAATCGGCCAACCACTGCCATGTGGTCCATCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGTAAAACGTGCATCCTCCAAGAACTGA

Protein sequence

MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIAFDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQRSSKPVKRASSKN
Homology
BLAST of Tan0001458 vs. ExPASy Swiss-Prot
Match: Q9SRE0 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Arabidopsis thaliana OX=3702 GN=COG0212 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 4.5e-131
Identity = 225/311 (72.35%), Postives = 265/311 (85.21%), Query Frame = 0

Query: 53  SKTQHGIAFDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLM 112
           S+   G+AFD  A+EA+R  LDA A + MAE + +++E   D DPKAWKWVIRK++WDLM
Sbjct: 44  SENGDGVAFDAVAYEADRLSLDAAAMEDMAETAKKELESDPDSDPKAWKWVIRKKMWDLM 103

Query: 113 ESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGG 172
           E++N A +PRPVHHRIPNFVGA  A  +L +L+ FR +  VKVNPDSPQK +R LTL+G 
Sbjct: 104 EARNYAMSPRPVHHRIPNFVGASAAARKLAELDAFRMAMVVKVNPDSPQKQIRFLTLSGE 163

Query: 173 KKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSV 232
           KKLLTPQPRLRTGFFS++ES +L P TI EACTSVGVAKYG+ IGLDEKIKVDLIVIGSV
Sbjct: 164 KKLLTPQPRLRTGFFSVLESDLLKPETIMEACTSVGVAKYGRAIGLDEKIKVDLIVIGSV 223

Query: 233 AVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVP 292
           AV+P+TGARLGKGEGFAELEYGMLRYMGAIDDSTP+VT+VHDCQLVDDIP+EKL +HDVP
Sbjct: 224 AVNPQTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTTVHDCQLVDDIPLEKLAIHDVP 283

Query: 293 VDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSE 352
           VDI+CTPT+VI TNT IPKPQGIYW+ LSPEKL Q+RILRELK R+E++ G+ LP GPSE
Sbjct: 284 VDIICTPTRVIFTNTPIPKPQGIYWDKLSPEKLRQIRILRELKNRLEKKTGRKLPTGPSE 343

Query: 353 KLPPTAQRSSK 364
           KLPPTA+R  +
Sbjct: 344 KLPPTAERKRR 354

BLAST of Tan0001458 vs. ExPASy Swiss-Prot
Match: Q0P464 (Methenyltetrahydrofolate synthase domain-containing protein OS=Danio rerio OX=7955 GN=mthfsd PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.2e-63
Identity = 122/245 (49.80%), Postives = 171/245 (69.80%), Query Frame = 0

Query: 101 KWVIRKRIWDLMESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSP 160
           KW +R ++W+ +E KN+A  PRPVH+RIPNF GA+EA N++  LE+F +S  VKV+PD P
Sbjct: 13  KWDVRHKVWNYIEVKNLANFPRPVHNRIPNFKGALEACNKVAQLEIFIESAVVKVDPDKP 72

Query: 161 QKGVRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLD 220
            +GVRL  L   K LL P PRLR G F+ I      T  T+    TS G+ ++  P+GLD
Sbjct: 73  MEGVRLAALKARKSLLVPTPRLRFGLFNRITPPKGATKETLRVCSTSQGIKEFSVPVGLD 132

Query: 221 EKIKVDLIVIGSVAVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVD 280
           +K++VDL+V+GSVAV  + G R+GKGEGFA++EY M+  MG++ +ST ++T VHDCQ++ 
Sbjct: 133 DKVQVDLVVVGSVAVSEK-GYRIGKGEGFADMEYAMMACMGSVTESTWVITVVHDCQVM- 192

Query: 281 DIPVEKLLVHDVPVDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIE 340
           DIP E +  HD+ VD + T T+VI T  K PKPQGI W ML  E+L ++ IL++L R +E
Sbjct: 193 DIPEELIERHDLMVDFIITATRVIKTECKHPKPQGIIWSMLHKEELKKIPILKKL-RTLE 252

Query: 341 REIGQ 345
           +E G+
Sbjct: 253 QEAGK 254

BLAST of Tan0001458 vs. ExPASy Swiss-Prot
Match: Q52L34 (Methenyltetrahydrofolate synthase domain-containing protein OS=Xenopus laevis OX=8355 GN=mthfsd PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 128/257 (49.81%), Postives = 169/257 (65.76%), Query Frame = 0

Query: 89  MEGAADDDPKAWKWVIRKRIWDLMESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFR 148
           ME     DP   KW IR+++WD +E+ N+A  PRPVHHRIPNF  + +A   + DLEVFR
Sbjct: 1   MEPGIRVDPGCSKWDIRQKVWDYIENNNLADFPRPVHHRIPNFKESHQACQNIIDLEVFR 60

Query: 149 DSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEAC-TSV 208
            +  VKV+PD P +GVRL  L   K LL P PRLRTG F+ +         +   C TS 
Sbjct: 61  RTWEVKVDPDKPLEGVRLAALQARKTLLVPTPRLRTGLFNKITPPPGANKEVLRICSTSQ 120

Query: 209 GVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTP 268
           GV  Y  P+GLD K++VDL+V+GSVAV  + G R+GKGEGFA++EY M+  MGA+ + T 
Sbjct: 121 GVKDYSVPMGLDAKVQVDLVVVGSVAVSVQ-GWRIGKGEGFADMEYAMMVSMGAVTEHTV 180

Query: 269 IVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQ 328
           +VT VHDCQ+V DIP E L  HD+ VD + TPT++I T+ K  KPQGI W M++ E + +
Sbjct: 181 VVTVVHDCQVV-DIPEELLEDHDLTVDFILTPTRIIKTDCKRAKPQGIIWSMITREMMGK 240

Query: 329 VRILRELKRRIEREIGQ 345
           + ILR L+ R ER  G+
Sbjct: 241 IPILRNLQDR-ERRAGK 254

BLAST of Tan0001458 vs. ExPASy Swiss-Prot
Match: Q2KI24 (Methenyltetrahydrofolate synthase domain-containing protein OS=Bos taurus OX=9913 GN=MTHFSD PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.2e-61
Identity = 127/261 (48.66%), Postives = 166/261 (63.60%), Query Frame = 0

Query: 104 IRKRIWDLMESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKG 163
           IR++IWD MES+N+A  PRPVHHRIPNF GA  A      L+ F+ ++ +KVNPD+PQK 
Sbjct: 12  IREQIWDYMESQNLADFPRPVHHRIPNFKGASRAAEHFPRLQAFKMARTIKVNPDAPQKN 71

Query: 164 VRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLDEKI 223
            R   L   K LL P PRLRTG F+ I      T   + +  TS GV  Y  P+GLD K+
Sbjct: 72  ARFFVLESKKTLLVPTPRLRTGLFNKITPPPGATKDILRKCATSQGVRNYSTPVGLDSKV 131

Query: 224 KVDLIVIGSVAVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIP 283
            VDL+V+GSVAV  + G R+GKGEG+A+LEY M+  MGA+   TP+VT VHDCQ+V DIP
Sbjct: 132 LVDLVVVGSVAVSEK-GWRIGKGEGYADLEYAMMVSMGAVSQGTPVVTIVHDCQVV-DIP 191

Query: 284 VEKLLVHDVPVDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREI 343
              L  HD+ VD + TPT+VI T  + PKP GI W  +S E L ++ ILR L+ + E + 
Sbjct: 192 EALLEDHDLTVDYILTPTRVITTGCERPKPAGIAWSKISCEMLGKMPILRSLRHQ-EEQA 251

Query: 344 GQPLPCGPSEKLPPTAQRSSK 364
           G+ +      + PP A RS +
Sbjct: 252 GKDVTLRDGPRSPPGATRSPR 269

BLAST of Tan0001458 vs. ExPASy Swiss-Prot
Match: Q2M296 (Methenyltetrahydrofolate synthase domain-containing protein OS=Homo sapiens OX=9606 GN=MTHFSD PE=1 SV=2)

HSP 1 Score: 235.3 bits (599), Expect = 1.1e-60
Identity = 130/264 (49.24%), Postives = 172/264 (65.15%), Query Frame = 0

Query: 104 IRKRIWDLMESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKG 163
           IR++IW  MES+N+A  PRPVHHRIPNF G+  A   + DL+VF  +Q VKV+PD P +G
Sbjct: 13  IREQIWGYMESQNLADFPRPVHHRIPNFKGSYLACQNIKDLDVFARTQEVKVDPDKPLEG 72

Query: 164 VRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLDEKI 223
           VRLL L   K LL P PRLRTG F+ I      T   + +  TS GV  Y  PIGLD ++
Sbjct: 73  VRLLVLQSKKTLLVPTPRLRTGLFNKITPPPGATKDILRKCATSQGVRNYSVPIGLDSRV 132

Query: 224 KVDLIVIGSVAVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIP 283
            VDL+V+GSVAV  + G R+GKGEG+A+LEY M+  MGA+   TP+VT VHDCQ+V DIP
Sbjct: 133 LVDLVVVGSVAVSEK-GWRIGKGEGYADLEYAMMVSMGAVSKETPVVTIVHDCQVV-DIP 192

Query: 284 VEKLLVHDVPVDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREI 343
            E +  HD+ VD + TPT+VI T  K PKP GI W  +S E + ++ ILR L+ R E++ 
Sbjct: 193 EELVEEHDITVDYILTPTRVIATGCKRPKPMGITWFKISLEMMEKIPILRSLRAR-EQQA 252

Query: 344 GQPLPC-GPSEKLPPTAQRSSKPV 366
           G+ +   G  + LP    + + P+
Sbjct: 253 GKDVTLQGEHQHLPEPGCQQTVPL 273

BLAST of Tan0001458 vs. NCBI nr
Match: XP_022939928.1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita moschata])

HSP 1 Score: 670.6 bits (1729), Expect = 7.7e-189
Identity = 341/372 (91.67%), Postives = 350/372 (94.09%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI +GRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA DDDPKAWKWVIRKRIWD MES+NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGALDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYW+MLSPEKLSQVRILRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWDMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. NCBI nr
Match: KAG6578879.1 (5-formyltetrahydrofolate cyclo-ligase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-188
Identity = 341/372 (91.67%), Postives = 350/372 (94.09%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI +GRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD E A+DDDPKAWKWVIRKRIWD MES+NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTESASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. NCBI nr
Match: XP_023551592.1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-188
Identity = 342/372 (91.94%), Postives = 351/372 (94.35%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI IGRNFQF+RHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFRRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA+DDDPKAWKWVIRKRIWD MES+NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. NCBI nr
Match: XP_022993523.1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita maxima])

HSP 1 Score: 668.7 bits (1724), Expect = 2.9e-188
Identity = 341/372 (91.67%), Postives = 350/372 (94.09%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI IGRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFQRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA+DDDPKAWKWVIRKRIWD MES NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESLNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYG+PIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGEPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVR+LRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRVLRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. NCBI nr
Match: KAG7016410.1 (5-formyltetrahydrofolate cyclo-ligase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 668.3 bits (1723), Expect = 3.8e-188
Identity = 340/372 (91.40%), Postives = 350/372 (94.09%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI +GRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD E A+DDDPKAWKWVIRKRIWD MES+NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTESASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLR+MGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRHMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. ExPASy TrEMBL
Match: A0A6J1FMW8 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445648 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.7e-189
Identity = 341/372 (91.67%), Postives = 350/372 (94.09%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI +GRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA DDDPKAWKWVIRKRIWD MES+NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGALDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYW+MLSPEKLSQVRILRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWDMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. ExPASy TrEMBL
Match: A0A6J1K2I1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489504 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.4e-188
Identity = 341/372 (91.67%), Postives = 350/372 (94.09%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI IGRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFQRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA+DDDPKAWKWVIRKRIWD MES NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESLNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYG+PIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGEPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVR+LRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRVLRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. ExPASy TrEMBL
Match: A0A6J1FIK2 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445648 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.2e-188
Identity = 339/372 (91.13%), Postives = 349/372 (93.82%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI +GRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA DDDPKAWKWVIRKRIWD MES+NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGALDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGK +GFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKVQGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYW+MLSPEKLSQVRILRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWDMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. ExPASy TrEMBL
Match: A0A6J1K0E7 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489504 PE=4 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.5e-187
Identity = 339/372 (91.13%), Postives = 349/372 (93.82%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSS LQLS PLSVKSTFLNAIRK+ SI SI IGRNFQFQRHFKLESS++   KTQ   A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFQRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEAERSRLDA A KSMAEAS+RD EGA+DDDPKAWKWVIRKRIWD MES NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESLNIAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYG+PIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGEPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGK +GFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKVQGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVR+LRELKRRIERE G+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRVLRELKRRIERETGKPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPGRRASSKN 372

BLAST of Tan0001458 vs. ExPASy TrEMBL
Match: A0A6J1BZW8 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Momordica charantia OX=3673 GN=LOC111006765 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.0e-186
Identity = 338/372 (90.86%), Postives = 349/372 (93.82%), Query Frame = 0

Query: 1   MDSSLLQLSHPLSVKSTFLNAIRKSRSISSISIGRNFQFQRHFKLESSRDNSSKTQHGIA 60
           MDSSLLQLSHPLS +STFLN IRKS S SSISIGRN QFQR+FKLESSR      +  +A
Sbjct: 1   MDSSLLQLSHPLSTRSTFLNVIRKSSSASSISIGRNSQFQRYFKLESSR-----ARGDVA 60

Query: 61  FDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLMESKNIAAN 120
           FDEAAFEA+RSRLDA+A+KSMAEASIR  EGA  DDPKAWKWVIRKRIWDLMES+N+AAN
Sbjct: 61  FDEAAFEADRSRLDAEAEKSMAEASIRATEGAPADDPKAWKWVIRKRIWDLMESQNVAAN 120

Query: 121 PRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFVGAMEA NRLCDLEVFRD+QCVKVNPDSPQKGVRLLTLTGGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDAQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGEGFAELEYGMLRYMGAIDDST IVTSVHDCQLVDD PVEKLLVHDVPVDIVCTP 
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTLIVTSVHDCQLVDDFPVEKLLVHDVPVDIVCTPK 300

Query: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSEKLPPTAQR 360
           QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERE GQPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGQPLPCGPSEKLPPTAQR 360

Query: 361 SSKPVKRASSKN 373
           SSKP +RASSKN
Sbjct: 361 SSKPTRRASSKN 367

BLAST of Tan0001458 vs. TAIR 10
Match: AT1G76730.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 469.2 bits (1206), Expect = 3.2e-132
Identity = 225/311 (72.35%), Postives = 265/311 (85.21%), Query Frame = 0

Query: 53  SKTQHGIAFDEAAFEAERSRLDAQAQKSMAEASIRDMEGAADDDPKAWKWVIRKRIWDLM 112
           S+   G+AFD  A+EA+R  LDA A + MAE + +++E   D DPKAWKWVIRK++WDLM
Sbjct: 44  SENGDGVAFDAVAYEADRLSLDAAAMEDMAETAKKELESDPDSDPKAWKWVIRKKMWDLM 103

Query: 113 ESKNIAANPRPVHHRIPNFVGAMEAVNRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGG 172
           E++N A +PRPVHHRIPNFVGA  A  +L +L+ FR +  VKVNPDSPQK +R LTL+G 
Sbjct: 104 EARNYAMSPRPVHHRIPNFVGASAAARKLAELDAFRMAMVVKVNPDSPQKQIRFLTLSGE 163

Query: 173 KKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIVIGSV 232
           KKLLTPQPRLRTGFFS++ES +L P TI EACTSVGVAKYG+ IGLDEKIKVDLIVIGSV
Sbjct: 164 KKLLTPQPRLRTGFFSVLESDLLKPETIMEACTSVGVAKYGRAIGLDEKIKVDLIVIGSV 223

Query: 233 AVDPRTGARLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVP 292
           AV+P+TGARLGKGEGFAELEYGMLRYMGAIDDSTP+VT+VHDCQLVDDIP+EKL +HDVP
Sbjct: 224 AVNPQTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTTVHDCQLVDDIPLEKLAIHDVP 283

Query: 293 VDIVCTPTQVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIEREIGQPLPCGPSE 352
           VDI+CTPT+VI TNT IPKPQGIYW+ LSPEKL Q+RILRELK R+E++ G+ LP GPSE
Sbjct: 284 VDIICTPTRVIFTNTPIPKPQGIYWDKLSPEKLRQIRILRELKNRLEKKTGRKLPTGPSE 343

Query: 353 KLPPTAQRSSK 364
           KLPPTA+R  +
Sbjct: 344 KLPPTAERKRR 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRE04.5e-13172.355-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Arabidopsis thalia... [more]
Q0P4645.2e-6349.80Methenyltetrahydrofolate synthase domain-containing protein OS=Danio rerio OX=79... [more]
Q52L341.3e-6149.81Methenyltetrahydrofolate synthase domain-containing protein OS=Xenopus laevis OX... [more]
Q2KI242.2e-6148.66Methenyltetrahydrofolate synthase domain-containing protein OS=Bos taurus OX=991... [more]
Q2M2961.1e-6049.24Methenyltetrahydrofolate synthase domain-containing protein OS=Homo sapiens OX=9... [more]
Match NameE-valueIdentityDescription
XP_022939928.17.7e-18991.675-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita... [more]
KAG6578879.11.0e-18891.675-formyltetrahydrofolate cyclo-ligase-like protein, partial [Cucurbita argyrospe... [more]
XP_023551592.11.0e-18891.945-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita... [more]
XP_022993523.12.9e-18891.675-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita... [more]
KAG7016410.13.8e-18891.405-formyltetrahydrofolate cyclo-ligase-like protein [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1FMW83.7e-18991.675-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbi... [more]
A0A6J1K2I11.4e-18891.675-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbi... [more]
A0A6J1FIK29.2e-18891.135-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbi... [more]
A0A6J1K0E73.5e-18791.135-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbi... [more]
A0A6J1BZW85.0e-18690.865-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
AT1G76730.13.2e-13272.35NagB/RpiA/CoA transferase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0026985-formyltetrahydrofolate cyclo-ligasePFAMPF018125-FTHF_cyc-ligcoord: 101..299
e-value: 3.3E-40
score: 137.9
IPR0026985-formyltetrahydrofolate cyclo-ligasePANTHERPTHR130175-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATEDcoord: 88..369
IPR0241855-formyltetrahydrofolate cyclo-ligase-like domain superfamilyGENE3D3.40.50.10420coord: 132..308
e-value: 2.3E-16
score: 61.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 342..372
IPR037171NagB/RpiA transferase-likeSUPERFAMILY100950NagB/RpiA/CoA transferase-likecoord: 100..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001458.1Tan0001458.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity