Tan0001427 (gene) Snake gourd v1

BLAST of Tan0001427 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 8.7e-26
Identity = 89/349 (25.50%), Postives = 147/349 (42.12%), Query Frame = 0

Query: 55  KSNGSVSSSQLSDQFDGQ---GLKVRSCIW--KWRGYSIRYQCSGDDGPALILIHGFGAN 114
           +S   V    L D+ +G+    +    C W  K R +  +  C   D PA++ + GFG  
Sbjct: 69  ESTSKVLIPGLPDESNGEIAARISHSHCEWKPKLRVHYEKAGCDNLDAPAVLFLPGFGVG 128

Query: 115 SDHWRKNIPALAESHRVFAIDLIG--------------------------YGYSDKPNPD 174
           S H+ K +  L   +RV+AID +G                          +G+ DK  P 
Sbjct: 129 SFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSSSEDKEPFWGFGDKTEP- 188

Query: 175 LVGESFYTFETWASQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICRGIVLLNIS 234
              +  ++ + W  Q+  F  +V+ +  +   NS+GG V L  A   P + +G+ LLN +
Sbjct: 189 WADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFAATHPHLVKGVTLLNAT 248

Query: 235 ---------LRMLHIKKQ-PWYGRPLIRSFQSLLRNTALGKYFFRTVATPEAVKNILCQC 294
                    +R   + +  PW G     +F    R   + +  ++ ++ PE++  IL Q 
Sbjct: 249 PFWGFFPNPVRSPKLARLFPWPG-----AFPLPERVKKITELVWQKISDPESIAEILKQV 308

Query: 295 YHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP-EELLPRVK---CPVLIGWG 354
           Y D S   D++   I+     P AA  F   +   GG L   E L R K     + + +G
Sbjct: 309 YTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCKENNVQICLMYG 368

Query: 355 DKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPLVKSFV 359
            +DPW     G+          +  I   GHCP DE P +VN L++ ++
Sbjct: 369 REDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYLMRGWI 411

BLAST of Tan0001427 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-18
Identity = 66/277 (23.83%), Postives = 124/277 (44.77%), Query Frame = 0

Query: 85  GYSIRYQCSGDDG-PALILIHGFGANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPD 144
           G ++ Y+   + G   L+ +HGF +++  +RK IP L + + + A+DL  +G S+K    
Sbjct: 14  GVNVYYEHYQNPGRQTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEKSRTF 73

Query: 145 LVGESFYTFETWASQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICRGIVLLNIS 204
           +     YT++  A  +      +   +A  + +S+GG + L AA+ KP++   +VLL  S
Sbjct: 74  I-----YTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSS 133

Query: 205 LRMLHIKKQPWYGRPLIRSFQSLLRNTALGKY--FFRTVATPEAVKNILCQCYHDTSQVT 264
                          L RS  +++  T +  +  + +   + E V   L    HD S + 
Sbjct: 134 -------------GYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGVMKNLLNVVHDKSLID 193

Query: 265 DELVQIILNPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIGWGDKDPWEPIELGR 324
           +E++     P            FI +  G L  E L ++  P L+ WG++D   P+E+G+
Sbjct: 194 EEMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGK 253

Query: 325 NYGSFESVEDFVVIPNVGHCPQDEAPHLVNPLVKSFV 359
              +         +   GH   +E P L++  +  F+
Sbjct: 254 RLHADLPNSVLYSLGQTGHLVPEERPELISEHIADFI 272

BLAST of Tan0001427 vs. ExPASy Swiss-Prot
Match: Q9AQM4 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase OS=Pseudomonas resinovorans OX=53412 GN=carC PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.2e-14
Identity = 78/294 (26.53%), Postives = 133/294 (45.24%), Query Frame = 0

Query: 85  GYSIRYQCSGDDGPALILIHGFGANSD---HWRKNIPALAESHRVFAIDLIGYGYSDKPN 144
           G   RY  +G  G  +ILIHG GA ++   +WR  IP LA  +RV A+D++G+G + KP+
Sbjct: 25  GVETRYLEAG-KGQPVILIHGGGAGAESEGNWRNVIPILARHYRVIAMDMLGFGKTAKPD 84

Query: 145 PDLVGESFYTFETWASQLNDFCVDVVQD-EAFFICNSIGGVVGLQAAVMKPQICRGIVLL 204
            +      YT +     L+DF   +  D +   + NS+GG  GL  +V+  ++   +VL+
Sbjct: 85  IE------YTQDRRIRHLHDFIKAMNFDGKVSIVGNSMGGATGLGVSVLHSELVNALVLM 144

Query: 205 NISLRMLHIKKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPEAVKNILCQCYHDTSQV 264
             +  ++ I +     RP+I              Y F    T E + +++    +D  ++
Sbjct: 145 GSAGLVVEIHEDL---RPII-------------NYDF----TREGMVHLVKALTNDGFKI 204

Query: 265 TDELVQIILNPGLQPGAADIF---LEFICYSGGPLPE-ELLPRVKCPVLIGWGDKDPWEP 324
            D ++               +   +++I   GG   + E + +V  P L+  G  D   P
Sbjct: 205 DDAMINSRYTYATDEATRKAYVATMQWIREQGGLFYDPEFIRKVPVPTLVVHGKDDKVVP 264

Query: 325 IELGRNYGSFESVEDF--VVIPNVGHCPQDEAPHLVNPLVKSFVSRHA--TRSA 367
           +E    Y   + ++D    +IP+ GH    E P        SF+SR A  TR+A
Sbjct: 265 VETA--YKFLDLIDDSWGYIIPHCGHWAMIEHPEDFANATLSFLSRRADITRAA 289

BLAST of Tan0001427 vs. ExPASy Swiss-Prot
Match: Q59695 (Dihydrolipoyllysine-residue acetyltransferase component of acetoin cleaving system OS=Pseudomonas putida OX=303 GN=acoC PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-14
Identity = 74/277 (26.71%), Postives = 118/277 (42.60%), Query Frame = 0

Query: 90  YQCSGDDGPALILIHGFGANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESF 149
           ++  G+ G  L+L+HGFG + ++W  N PALA   RV A+DL G+G S K      G+  
Sbjct: 126 FELGGEGGTPLVLVHGFGGDLNNWLFNHPALAAERRVIALDLPGHGESAKALQR--GDLD 185

Query: 150 YTFETWASQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHI 209
              ET  + L+   +D+   +A    +S+GG V L  A + PQ    + L+         
Sbjct: 186 ELSETVLALLDH--LDIA--KAHLAGHSMGGAVSLNVAGLAPQRVASLSLI--------- 245

Query: 210 KKQPWYGRPLIRSFQSLLRNTALGKYF--FRTVATPEAVKNILCQCYHDTSQVTDELVQI 269
                          + L     G+Y   F   A   A+K  + Q + D + VT ++++ 
Sbjct: 246 -------------ASAGLGEAINGQYLQGFVAAANRNALKPQMVQLFADPALVTRQMLED 305

Query: 270 ILNPGLQPGAADIFLEF-ICYSGGPLPEELLPRV--KCPVLIGWGDKDPWEPIELGRNYG 329
           +L      G  +   +  +  + G      L  V  + P L+ WG KD   P    R   
Sbjct: 306 MLKFKRLEGVDEALRQLALAIADGDRQRHDLRSVLGQHPALVVWGGKDAIIPASHARKGP 365

Query: 330 SFESVEDFVVIPNVGHCPQDEAPHLVNPLVKSFVSRH 362
             E     +V+P  GH  Q EA   VN  + +F+ +H
Sbjct: 366 EAE----VLVLPEAGHMVQMEAAEQVNQQMLAFLRKH 370

BLAST of Tan0001427 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 1.1e-12
Identity = 73/333 (21.92%), Postives = 136/333 (40.84%), Query Frame = 0

Query: 47  RAPAFSPIKSNGSVSSSQLSDQFDGQGLKVRSCIWKWRGYSIRYQCSGDDG-PALILIHG 106
           RAPA +  ++  +   + L+D   G    VR    K  G    Y  +G+ G P ++L+HG
Sbjct: 43  RAPAQTFRRAARANPPACLNDPSLGTHCYVRI---KDSGLRFHYVAAGERGKPLMLLLHG 102

Query: 107 FGANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVD 166
           F      WR  +      +RV A+DL GYG SD P      +  Y  +   + + D    
Sbjct: 103 FPEFWYSWRHQLREFKSEYRVVALDLRGYGESDAP----AHQESYKLDCLIADIKDILDS 162

Query: 167 VVQDEAFFICNSIGGVVGLQAAVMKPQICRGIVLLNI---SLRMLHIKKQP--------- 226
           +   +   I +  GG++    AV  P++   ++++N    S+   +I + P         
Sbjct: 163 LGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSVFTEYILRHPAQLFRSSFY 222

Query: 227 -WYGRPLIRSFQSLLRNTALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPG 286
            ++  P    F   + +    K+ F + +T    K         T++  +  V +   PG
Sbjct: 223 YFFQIPRFPEFMFSINDFKALKHLFTSQSTGIGRKG-----RQLTTEDLEAYVYVFSQPG 282

Query: 287 LQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSF-ESVED 346
              G  + +     +S  PL   +   V  P L+ WG++D +  +E+      + ++   
Sbjct: 283 ALSGPINHYRNI--FSCLPLKHHM---VTTPTLLLWGEEDAFMEVEMAEVTKIYVKNYFR 342

Query: 347 FVVIPNVGHCPQDEAPHLVNPLVKSFVSRHATR 365
             ++    H  Q + P +VN L+ +F+     R
Sbjct: 343 LTILSEGSHWLQQDQPDIVNGLIWAFLKEETRR 358

BLAST of Tan0001427 vs. NCBI nr
Match: XP_022958555.1 (pheophytinase, chloroplastic [Cucurbita moschata] >KAG6605837.1 Pheophytinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 681.8 bits (1758), Expect = 3.3e-192
Identity = 330/383 (86.16%), Postives = 345/383 (90.08%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+   SS+LPF KLPP  SS+KVTA  L S  T  HSFS QLG P RAPAF PIKSNGS+
Sbjct: 1   MAGALSSSLPFSKLPPSISSSKVTAGVLPSSLTFTHSFSPQLGLPRRAPAFYPIKSNGSL 60

Query: 61  SSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGAN 120
            SSQ  DQFD QG           L+VRSCIW WRGYSIRYQC+GD GPALILIHGFGAN
Sbjct: 61  LSSQFGDQFDDQGLQISSENTRSQLQVRSCIWNWRGYSIRYQCAGDHGPALILIHGFGAN 120

Query: 121 SDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180
           SDHWRKNIPALAESHRV+A+DLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 181 EAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 240
           EAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 241 TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 300
           TALGKYFF TVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFTTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 301 GPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHL 360
           GPLPEELLP VKCPVLIGWGDKDPWEPIELGRNY +FESVEDFVVIPNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYENFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 361 VNPLVKSFVSRHATRSARTSSAV 373
           VNPLV+SFV+RHATRSARTSS V
Sbjct: 361 VNPLVESFVTRHATRSARTSSVV 383

BLAST of Tan0001427 vs. NCBI nr
Match: XP_022995331.1 (pheophytinase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 679.9 bits (1753), Expect = 1.3e-191
Identity = 329/383 (85.90%), Postives = 345/383 (90.08%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+   SS+LPF KLPP  SS+KVTA  L S  T  HSFS QLG P RAPAF PIKSNGS+
Sbjct: 1   MAGALSSSLPFSKLPPSISSSKVTAGVLPSSLTFTHSFSPQLGLPRRAPAFYPIKSNGSL 60

Query: 61  SSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGAN 120
            SSQ  DQF+ QG           L+VRSCIW WRGYSIRYQCSGD GPALILIHGFGAN
Sbjct: 61  LSSQFGDQFEDQGLQISSENTRSQLQVRSCIWNWRGYSIRYQCSGDHGPALILIHGFGAN 120

Query: 121 SDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180
           SDHWRKNIPALAESHRV+A+DLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 181 EAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 240
           EAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 241 TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 300
           TALGKYFF+TVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFKTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 301 GPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHL 360
           GPLPEELLP VKCPVLIGWGDKDPWEPIELGRNY +FESVEDFVVIPNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYKNFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 361 VNPLVKSFVSRHATRSARTSSAV 373
           VNPLV+SFV+RHAT SAR SSAV
Sbjct: 361 VNPLVESFVTRHATSSARNSSAV 383

BLAST of Tan0001427 vs. NCBI nr
Match: XP_023533508.1 (pheophytinase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674.9 bits (1740), Expect = 4.1e-190
Identity = 328/383 (85.64%), Postives = 343/383 (89.56%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+   SS+LPF KL P  SS+KV A  L S  T   SFS QLG P RAPAF PIKSNGS+
Sbjct: 1   MAGALSSSLPFSKLRPSISSSKVVAGVLPSSLTFTPSFSPQLGLPRRAPAFYPIKSNGSL 60

Query: 61  SSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGAN 120
            SSQ  DQFD QG           L+VRSCIW WRGYSIRYQC+GD GPALILIHGFGAN
Sbjct: 61  LSSQFGDQFDDQGLQISLENTRSQLQVRSCIWNWRGYSIRYQCAGDHGPALILIHGFGAN 120

Query: 121 SDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180
           SDHWRKNIPALAESHRV+A+DLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 181 EAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 240
           EAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 241 TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 300
           TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 301 GPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHL 360
           GPLPEELLP VKCPVLIGWGDKDPWEPIELGRNY +FESVEDFVVIPNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYKNFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 361 VNPLVKSFVSRHATRSARTSSAV 373
           VNPLV+SFV+RHATRSARTSS V
Sbjct: 361 VNPLVESFVTRHATRSARTSSVV 383

BLAST of Tan0001427 vs. NCBI nr
Match: XP_022153858.1 (pheophytinase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 648.7 bits (1672), Expect = 3.1e-182
Identity = 322/387 (83.20%), Postives = 339/387 (87.60%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPA--FSPIKSNG 60
           M+T  SSTLPF KLP PFSSAK + S         H F QQL FPLRAP   F+ +KS G
Sbjct: 1   MATACSSTLPFSKLPLPFSSAKPSISI--------HPFLQQLRFPLRAPTFPFARLKSKG 60

Query: 61  SVSSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFG 120
           +V SSQL   F+  G           L+V+S IWKW+GYSIRYQCSGDDGPAL+LIHGFG
Sbjct: 61  TVLSSQLRVHFEDHGLETSSDNIHSQLQVKSRIWKWKGYSIRYQCSGDDGPALVLIHGFG 120

Query: 121 ANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVV 180
           ANSDHWRKNI ALAESHRV+A+DLIGYGYSDKPNPD+VGESFYTFETWA+QLNDFCVDVV
Sbjct: 121 ANSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVV 180

Query: 181 QDEAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLL 240
           QDEAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYGRPLIRSFQSLL
Sbjct: 181 QDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLL 240

Query: 241 RNTALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICY 300
           RNTALGKYFFRTVATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICY
Sbjct: 241 RNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICY 300

Query: 301 SGGPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAP 360
           SGGPLPEELLPRVKCPVLI WGDKDPWEPIELGRNY SFESVEDFVV+PNVGHCPQDEAP
Sbjct: 301 SGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAP 360

Query: 361 HLVNPLVKSFVSRHATRSAR--TSSAV 373
           HLVNPLVKSFVSRHATRSA   TSS V
Sbjct: 361 HLVNPLVKSFVSRHATRSASACTSSGV 379

BLAST of Tan0001427 vs. NCBI nr
Match: KAA0040105.1 (pheophytinase [Cucumis melo var. makuwa])

HSP 1 Score: 647.9 bits (1670), Expect = 5.3e-182
Identity = 314/373 (84.18%), Postives = 331/373 (88.74%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+TI S TLPF KLP PFSSAK+T     SP T  H FS      LR+PAFSPIK N +V
Sbjct: 1   MTTICSFTLPFSKLPLPFSSAKLTTGTTPSPLTSTHPFS----LSLRSPAFSPIKPNATV 60

Query: 61  SSSQLSDQ-----FDG--QGLKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW 120
           SSSQ  DQ      D     L +RSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW
Sbjct: 61  SSSQFGDQGLQISSDSICSQLPIRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW 120

Query: 121 RKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQDEAFF 180
           RKNIP LA+SHRV+AIDLIGYGYSDKPNP+LVGE FYTFETWASQLNDFCVDVVQD+AFF
Sbjct: 121 RKNIPVLAQSHRVYAIDLIGYGYSDKPNPELVGEGFYTFETWASQLNDFCVDVVQDDAFF 180

Query: 181 ICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNTALG 240
           ICNSIGGVVGLQAA+MKPQIC GIVLLNISLRMLHIKKQPWYG+P IRSFQ+LLRNTALG
Sbjct: 181 ICNSIGGVVGLQAAIMKPQICNGIVLLNISLRMLHIKKQPWYGKPFIRSFQNLLRNTALG 240

Query: 241 KYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP 300
           KYFFR VATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP
Sbjct: 241 KYFFRAVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP 300

Query: 301 EELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPL 360
           EELLPRVKCPVLI WGDKDPWEPIELGRNYGSFESVE+FVV+PNVGHCPQDEAPHLVNPL
Sbjct: 301 EELLPRVKCPVLIAWGDKDPWEPIELGRNYGSFESVEEFVVLPNVGHCPQDEAPHLVNPL 360

Query: 361 VKSFVSRHATRSA 367
           V+SFVS HATR+A
Sbjct: 361 VESFVSGHATRNA 369

BLAST of Tan0001427 vs. ExPASy TrEMBL
Match: A0A6J1H3U7 (pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111459754 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 330/383 (86.16%), Postives = 345/383 (90.08%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+   SS+LPF KLPP  SS+KVTA  L S  T  HSFS QLG P RAPAF PIKSNGS+
Sbjct: 1   MAGALSSSLPFSKLPPSISSSKVTAGVLPSSLTFTHSFSPQLGLPRRAPAFYPIKSNGSL 60

Query: 61  SSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGAN 120
            SSQ  DQFD QG           L+VRSCIW WRGYSIRYQC+GD GPALILIHGFGAN
Sbjct: 61  LSSQFGDQFDDQGLQISSENTRSQLQVRSCIWNWRGYSIRYQCAGDHGPALILIHGFGAN 120

Query: 121 SDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180
           SDHWRKNIPALAESHRV+A+DLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 181 EAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 240
           EAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 241 TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 300
           TALGKYFF TVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFTTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 301 GPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHL 360
           GPLPEELLP VKCPVLIGWGDKDPWEPIELGRNY +FESVEDFVVIPNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYENFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 361 VNPLVKSFVSRHATRSARTSSAV 373
           VNPLV+SFV+RHATRSARTSS V
Sbjct: 361 VNPLVESFVTRHATRSARTSSVV 383

BLAST of Tan0001427 vs. ExPASy TrEMBL
Match: A0A6J1K1N6 (pheophytinase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111490908 PE=4 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 6.1e-192
Identity = 329/383 (85.90%), Postives = 345/383 (90.08%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+   SS+LPF KLPP  SS+KVTA  L S  T  HSFS QLG P RAPAF PIKSNGS+
Sbjct: 1   MAGALSSSLPFSKLPPSISSSKVTAGVLPSSLTFTHSFSPQLGLPRRAPAFYPIKSNGSL 60

Query: 61  SSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGAN 120
            SSQ  DQF+ QG           L+VRSCIW WRGYSIRYQCSGD GPALILIHGFGAN
Sbjct: 61  LSSQFGDQFEDQGLQISSENTRSQLQVRSCIWNWRGYSIRYQCSGDHGPALILIHGFGAN 120

Query: 121 SDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180
           SDHWRKNIPALAESHRV+A+DLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 181 EAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 240
           EAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 241 TALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 300
           TALGKYFF+TVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFKTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 301 GPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHL 360
           GPLPEELLP VKCPVLIGWGDKDPWEPIELGRNY +FESVEDFVVIPNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYKNFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 361 VNPLVKSFVSRHATRSARTSSAV 373
           VNPLV+SFV+RHAT SAR SSAV
Sbjct: 361 VNPLVESFVTRHATSSARNSSAV 383

BLAST of Tan0001427 vs. ExPASy TrEMBL
Match: A0A6J1DIP0 (pheophytinase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021274 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 322/387 (83.20%), Postives = 339/387 (87.60%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPA--FSPIKSNG 60
           M+T  SSTLPF KLP PFSSAK + S         H F QQL FPLRAP   F+ +KS G
Sbjct: 1   MATACSSTLPFSKLPLPFSSAKPSISI--------HPFLQQLRFPLRAPTFPFARLKSKG 60

Query: 61  SVSSSQLSDQFDGQG-----------LKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFG 120
           +V SSQL   F+  G           L+V+S IWKW+GYSIRYQCSGDDGPAL+LIHGFG
Sbjct: 61  TVLSSQLRVHFEDHGLETSSDNIHSQLQVKSRIWKWKGYSIRYQCSGDDGPALVLIHGFG 120

Query: 121 ANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVV 180
           ANSDHWRKNI ALAESHRV+A+DLIGYGYSDKPNPD+VGESFYTFETWA+QLNDFCVDVV
Sbjct: 121 ANSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVV 180

Query: 181 QDEAFFICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLL 240
           QDEAFFICNSIGGVVGLQAAVMKPQIC+GIVLLNISLRMLHIKKQPWYGRPLIRSFQSLL
Sbjct: 181 QDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLL 240

Query: 241 RNTALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICY 300
           RNTALGKYFFRTVATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICY
Sbjct: 241 RNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICY 300

Query: 301 SGGPLPEELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAP 360
           SGGPLPEELLPRVKCPVLI WGDKDPWEPIELGRNY SFESVEDFVV+PNVGHCPQDEAP
Sbjct: 301 SGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAP 360

Query: 361 HLVNPLVKSFVSRHATRSAR--TSSAV 373
           HLVNPLVKSFVSRHATRSA   TSS V
Sbjct: 361 HLVNPLVKSFVSRHATRSASACTSSGV 379

BLAST of Tan0001427 vs. ExPASy TrEMBL
Match: A0A5A7TFB8 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00800 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 314/373 (84.18%), Postives = 331/373 (88.74%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+TI S TLPF KLP PFSSAK+T     SP T  H FS      LR+PAFSPIK N +V
Sbjct: 1   MTTICSFTLPFSKLPLPFSSAKLTTGTTPSPLTSTHPFS----LSLRSPAFSPIKPNATV 60

Query: 61  SSSQLSDQ-----FDG--QGLKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW 120
           SSSQ  DQ      D     L +RSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW
Sbjct: 61  SSSQFGDQGLQISSDSICSQLPIRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW 120

Query: 121 RKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQDEAFF 180
           RKNIP LA+SHRV+AIDLIGYGYSDKPNP+LVGE FYTFETWASQLNDFCVDVVQD+AFF
Sbjct: 121 RKNIPVLAQSHRVYAIDLIGYGYSDKPNPELVGEGFYTFETWASQLNDFCVDVVQDDAFF 180

Query: 181 ICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNTALG 240
           ICNSIGGVVGLQAA+MKPQIC GIVLLNISLRMLHIKKQPWYG+P IRSFQ+LLRNTALG
Sbjct: 181 ICNSIGGVVGLQAAIMKPQICNGIVLLNISLRMLHIKKQPWYGKPFIRSFQNLLRNTALG 240

Query: 241 KYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP 300
           KYFFR VATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP
Sbjct: 241 KYFFRAVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP 300

Query: 301 EELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPL 360
           EELLPRVKCPVLI WGDKDPWEPIELGRNYGSFESVE+FVV+PNVGHCPQDEAPHLVNPL
Sbjct: 301 EELLPRVKCPVLIAWGDKDPWEPIELGRNYGSFESVEEFVVLPNVGHCPQDEAPHLVNPL 360

Query: 361 VKSFVSRHATRSA 367
           V+SFVS HATR+A
Sbjct: 361 VESFVSGHATRNA 369

BLAST of Tan0001427 vs. ExPASy TrEMBL
Match: A0A5D3DEA7 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001020 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 314/373 (84.18%), Postives = 331/373 (88.74%), Query Frame = 0

Query: 1   MSTIFSSTLPFFKLPPPFSSAKVTASFLASPPTPRHSFSQQLGFPLRAPAFSPIKSNGSV 60
           M+TI S TLPF KLP PFSSAK+T     SP T  H FS      LR+PAFSPIK N +V
Sbjct: 3   MTTICSFTLPFSKLPLPFSSAKLTTGTTPSPLTSTHPFS----LSLRSPAFSPIKPNATV 62

Query: 61  SSSQLSDQ-----FDG--QGLKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW 120
           SSSQ  DQ      D     L +RSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW
Sbjct: 63  SSSQFGDQGLQISSDSICSQLPIRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHW 122

Query: 121 RKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQDEAFF 180
           RKNIP LA+SHRV+AIDLIGYGYSDKPNP+LVGE FYTFETWASQLNDFCVDVVQD+AFF
Sbjct: 123 RKNIPVLAQSHRVYAIDLIGYGYSDKPNPELVGEGFYTFETWASQLNDFCVDVVQDDAFF 182

Query: 181 ICNSIGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNTALG 240
           ICNSIGGVVGLQAA+MKPQIC GIVLLNISLRMLHIKKQPWYG+P IRSFQ+LLRNTALG
Sbjct: 183 ICNSIGGVVGLQAAIMKPQICNGIVLLNISLRMLHIKKQPWYGKPFIRSFQNLLRNTALG 242

Query: 241 KYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP 300
           KYFFR VATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP
Sbjct: 243 KYFFRAVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP 302

Query: 301 EELLPRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPL 360
           EELLPRVKCPVLI WGDKDPWEPIELGRNYGSFESVE+FVV+PNVGHCPQDEAPHLVNPL
Sbjct: 303 EELLPRVKCPVLIAWGDKDPWEPIELGRNYGSFESVEEFVVLPNVGHCPQDEAPHLVNPL 362

Query: 361 VKSFVSRHATRSA 367
           V+SFVS HATR+A
Sbjct: 363 VESFVSGHATRNA 371

BLAST of Tan0001427 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 4.7e-144
Identity = 223/304 (73.36%), Postives = 268/304 (88.16%), Query Frame = 0

Query: 59  SVSSSQLSDQFDGQGLKVRSCIWKWRGYSIRYQCSGDDGPALILIHGFGANSDHWRKNIP 118
           SVS S ++D+   + L+VR+  WKW+GYSIRYQC+G  GPAL+L+HGFGANSDHWRKN P
Sbjct: 56  SVSCSSVTDEASSEELQVRTLTWKWKGYSIRYQCAGTSGPALVLVHGFGANSDHWRKNTP 115

Query: 119 ALAESHRVFAIDLIGYGYSDKPNP-DLVGESFYTFETWASQLNDFCVDVVQDEAFFICNS 178
            L ++HRV++IDLIGYGYSDKPNP +  GE FYTFETW  QLNDFC+DVV+DEAFFICNS
Sbjct: 116 ILGKTHRVYSIDLIGYGYSDKPNPREFGGEPFYTFETWGEQLNDFCLDVVKDEAFFICNS 175

Query: 179 IGGVVGLQAAVMKPQICRGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNTALGKYFF 238
           IGG+VGLQAAV KP+ICRG++L+NISLRMLHIKKQP+ GRP I+SFQ+LLRNT +GK FF
Sbjct: 176 IGGLVGLQAAVSKPEICRGLMLINISLRMLHIKKQPFIGRPFIKSFQNLLRNTPVGKLFF 235

Query: 239 RTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLPEELL 298
           +++A PE VK+ILCQCYHD+SQVTDELV+ IL PGL+PGA D+FLEFICYSGGPLPE+LL
Sbjct: 236 KSIAKPETVKSILCQCYHDSSQVTDELVEAILRPGLEPGAVDVFLEFICYSGGPLPEDLL 295

Query: 299 PRVKCPVLIGWGDKDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPLVKSF 358
           P VKCPVLI WG+KDPWEPIELGR Y +F++VEDFVV+P+ GHCPQDE P +VNPL++SF
Sbjct: 296 PLVKCPVLIAWGEKDPWEPIELGRAYSNFDAVEDFVVLPDAGHCPQDEKPEMVNPLIESF 355

Query: 359 VSRH 362
           V+RH
Sbjct: 356 VARH 359

BLAST of Tan0001427 vs. TAIR 10
Match: AT5G38520.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 8.5e-45
Identity = 112/315 (35.56%), Postives = 170/315 (53.97%), Query Frame = 0

Query: 56  SNGSVSSSQLSDQFDGQGLKVRSCIWKWRG-YSIRYQCSGDDGP--------ALILIHGF 115
           S+ +VS   + +  +   +  RS  WKW+G YS+ Y     D P         ++L+HGF
Sbjct: 42  SSATVSGGGVVEAVELAEIGERSKKWKWKGEYSVNYFVK--DSPEEVTPASQTVLLVHGF 101

Query: 116 GANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDV 175
           GA+  HWR+NI AL+++H V+AIDL+G+G SDKP     G S YT E+WA  + +F  +V
Sbjct: 102 GASIPHWRRNINALSKNHTVYAIDLLGFGASDKP----PGFS-YTMESWAELILNFLEEV 161

Query: 176 VQDEAFFICNSIGGVV-GLQAAVMKPQICRGIVLLNISLRMLH-IKKQPWYGR---PLIR 235
           VQ     I NS+G +   + A+  +  + +G+VLLN +  M +      W  +   PL+ 
Sbjct: 162 VQKPTILIGNSVGSLACVIAASESRGDLVKGLVLLNCAGGMNNKAVFDDWRIKLLMPLLL 221

Query: 236 SFQSLLRNTALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIF 295
               LL+   +    F  V   E +KNIL   Y +   V D LV+II  P    GA D F
Sbjct: 222 LIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIAGPANTEGALDAF 281

Query: 296 LEFICYSGGPLPEELLPRVKCPVLIGWGDKDPWEPIE--LGRNYGSF-ESVEDF--VVIP 352
           +  +    GP P +L+P +  PVL+ WGD+D   P++  +G+ + S  + + +F   V+ 
Sbjct: 282 VSILTGPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPVGKYFTSLPDQLPNFNLYVLQ 341

BLAST of Tan0001427 vs. TAIR 10
Match: AT5G38520.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.1e-43
Identity = 113/327 (34.56%), Postives = 170/327 (51.99%), Query Frame = 0

Query: 56  SNGSVSSSQLSDQFDGQGLKVRSCIWKWRG-YSIRYQCSGDDGP--------ALILIHGF 115
           S+ +VS   + +  +   +  RS  WKW+G YS+ Y     D P         ++L+HGF
Sbjct: 42  SSATVSGGGVVEAVELAEIGERSKKWKWKGEYSVNYFVK--DSPEEVTPASQTVLLVHGF 101

Query: 116 GANSDHWRKNIPALAESHRVFAIDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDV 175
           GA+  HWR+NI AL+++H V+AIDL+G+G SDKP     G S YT E+WA  + +F  +V
Sbjct: 102 GASIPHWRRNINALSKNHTVYAIDLLGFGASDKP----PGFS-YTMESWAELILNFLEEV 161

Query: 176 VQDEAFFICNSIGGVVGLQAA-------------VMKPQICRGIVLLNISLRMLH-IKKQ 235
           VQ     I NS+G +  + AA               +  + +G+VLLN +  M +     
Sbjct: 162 VQKPTILIGNSVGSLACVIAASGTKFLIYLEKKTESRGDLVKGLVLLNCAGGMNNKAVFD 221

Query: 236 PWYGR---PLIRSFQSLLRNTALGKYFFRTVATPEAVKNILCQCYHDTSQVTDELVQIIL 295
            W  +   PL+     LL+   +    F  V   E +KNIL   Y +   V D LV+II 
Sbjct: 222 DWRIKLLMPLLLLIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIA 281

Query: 296 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIGWGDKDPWEPIE--LGRNYGSF- 352
            P    GA D F+  +    GP P +L+P +  PVL+ WGD+D   P++  +G+ + S  
Sbjct: 282 GPANTEGALDAFVSILTGPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPVGKYFTSLP 341

BLAST of Tan0001427 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.7e-43
Identity = 93/289 (32.18%), Postives = 146/289 (50.52%), Query Frame = 0

Query: 81  WKWRGYSIRYQCSGDDGPALILIHGFGANSDHWRKNIPALAESHRVFAIDLIGYGYSDKP 140
           W+WRG+ I Y   G+  P L+LIHGFGA+  HWR NIP LA+ ++V+A+DL+G+G+SDK 
Sbjct: 27  WEWRGHKIHYVVQGEGSP-LVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWSDKA 86

Query: 141 NPDLVGESFYTFETWASQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICRGIVLL 200
             +      Y    W  Q+ DF  +VV++ A  + NS+GG   L  AV  P+   G+ LL
Sbjct: 87  LIE------YDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALL 146

Query: 201 N------ISLRMLHIKKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPEAVKNILCQCY 260
           N         R      +    + +++  + + +   LG + F     P  ++++L   Y
Sbjct: 147 NSAGQFAAESRKREEADETVITKFIVKPLKEIFQRVVLG-FLFWQAKQPSRIESVLKSVY 206

Query: 261 HDTSQVTDELVQIILNPGLQPGAADIF----LEFICYSGGPLPEELLPRVKCPVLIGWGD 320
            D++ V D LV+ I  P   P A +++      F+        + +L ++ CP+L+ WGD
Sbjct: 207 IDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGD 266

Query: 321 KDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPLVKSFVS 360
            DPW          +F S    V +   GHCP DE P  VN  +  ++S
Sbjct: 267 LDPWVGPAKAEKIKAFYSNSSLVHL-QAGHCPHDEVPEAVNKALLDWLS 306

BLAST of Tan0001427 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.7e-43
Identity = 93/289 (32.18%), Postives = 146/289 (50.52%), Query Frame = 0

Query: 81  WKWRGYSIRYQCSGDDGPALILIHGFGANSDHWRKNIPALAESHRVFAIDLIGYGYSDKP 140
           W+WRG+ I Y   G+  P L+LIHGFGA+  HWR NIP LA+ ++V+A+DL+G+G+SDK 
Sbjct: 84  WEWRGHKIHYVVQGEGSP-LVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWSDKA 143

Query: 141 NPDLVGESFYTFETWASQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICRGIVLL 200
             +      Y    W  Q+ DF  +VV++ A  + NS+GG   L  AV  P+   G+ LL
Sbjct: 144 LIE------YDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALL 203

Query: 201 N------ISLRMLHIKKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPEAVKNILCQCY 260
           N         R      +    + +++  + + +   LG + F     P  ++++L   Y
Sbjct: 204 NSAGQFAAESRKREEADETVITKFIVKPLKEIFQRVVLG-FLFWQAKQPSRIESVLKSVY 263

Query: 261 HDTSQVTDELVQIILNPGLQPGAADIF----LEFICYSGGPLPEELLPRVKCPVLIGWGD 320
            D++ V D LV+ I  P   P A +++      F+        + +L ++ CP+L+ WGD
Sbjct: 264 IDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGD 323

Query: 321 KDPWEPIELGRNYGSFESVEDFVVIPNVGHCPQDEAPHLVNPLVKSFVS 360
            DPW          +F S    V +   GHCP DE P  VN  +  ++S
Sbjct: 324 LDPWVGPAKAEKIKAFYSNSSLVHL-QAGHCPHDEVPEAVNKALLDWLS 363