Tan0001359 (gene) Snake gourd v1

Overview
NameTan0001359
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPyruvate kinase
LocationLG01: 1571450 .. 1577327 (-)
RNA-Seq ExpressionTan0001359
SyntenyTan0001359
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAACGAAGCCTAACCGCGCTACTAGCTTCCCATTTCTTCGATACAACCGAAGCTCTCTCTCACTCACACATTGCTAACTCCGCCTGAGGATTCTGGCGAAATTTTCTACGTTCAACTCATCAAGCATTTCTCCGTTCGTTTCGCCCTCTGTTAAATCTCTTCCTCGGGAGCTTTCTTGTTCAGTTTCTCTCTCTGTTTTTGCTTCTGTTCGGAAAATGTCTCAGGTGGTGGCTACGAGGTCGATTCACAATACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGCTGGACAGAGCTCGGTCTTTTGGTTTTGGCTCCAAAGTGTTGGCGCATGAGAAGAAGAGTTGGAATTTTGTTTGCAGGAAAAGTCTAATCACATCCAAGAGAGCTGCTCAAGCTGAAGTTGTTCCGGTATCACCTGAGGATGTTCAGAAGGTTTGTGTGATCTTCTGTTTTGTTAATTTTGTTTTTAAGGAAAGTGCTTTTATTTGCTTGTTACGATAAGATTTTGAGGGAAAATGGGGAGCTGGAGGGAGTCATGGAATTTGAATGTTTAAAAGACTGGGATTAAAGAATTATGGAGTTGTTTGAAAGGCACGTAGCATTCAGGAAGCAAAATGGTGTACTTTATTTGTATAGCGTCGAGTTGTAGATCCGTAATTTGGTATAGCTCCGGCATTTTGATGCCTAGAGTTTTTGTACGGCTGGAAATTTAGTATGCCTGTTTCTGTCTCTGAGAAAGCGGAGTATCAATAAATGGCTTTTCTTTGATCTCTGAGTTTTCGGAAGGGAACGTGCATACACACTCGTAAATTTAATTTTTTATATGTATTTACGCGGTTGCTCGAGAAGCCTCTTCTAGTCCTTTTACTTTGTTTAAATATGAAAATGGATATTTTGTAGCCTTCACTGGCTGCAAATGTAAGAGAATTCTAATTTTCATGTGTTAATATGATTCTGAATTTTGCTTATCAGGCAGACGAGCAATTTGAACATTTACGAGCAGTTCAACAACTCGGCGATACACCTGTTGGAATGTGGTCAAAACCAACAGTTAGACGCAAGACAAAAATCGTGTGCACAATTGGACCTTCTACTAACACGAAAGAAATGATTTGGAAACTGGCTGAGGCTGGAATGAATGTTGCCCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGGGAATACAATGCCCAATCACAGGACAACTGCATTGCAATCATGCTTGACACCAAGGCAAGTTCATTTTCATATTTCCCCATGTTCAACACCTTCGATTCATTAACCTAGCAACAAACATTTTCCCAGATAACAAGTTTCATTTTCCTGGAATTTGCAAATATTGTCTTTTCCATCTTTACCCTGCCAGTCTGAATAATAGCATAACCTTGACCACCAAGGTTACTGGAATGTTCTAAAAACAAGTCTATAGTTTGAACTCTACAATAGGCTTTGTGTTATATTGCTTCAATATTTTTTGATTCTGATATGTAGCTTGCTCTTTTGCCTTGTGCTTTAACTTGCTTTTTAGAACTCAGGTCATTCCAATCTTTTCTCTATCAAAGTTGAAAATATAAATTAAGTGCTAATTTCATTGAAAAACTTTTGTTGTTATTTATTTTTGTTTCTTTTAAGGTTTGATTTTTTCTTCCAAGACTGCGCTCGGGATAAACAAAAACTCTTCGACTTTATCATTTTTTTAGCTCTCTCATGGTGTAAATTAACCCCTCACTTTTGTGATTACAGTCTCACTTCTTTTGTTAATAGCTGAGACTCTCTTTTGTAATGGGTTCTCTACCCTTTCTTTTGTACATGTCATACCATCAATGAAATGATTACTCATGTGTTCTCATAAAAAAAAAAAAGGTTTGATTTTTTCTTGTGCTCAATATTGGTAAATGGTAGATTTGATTCACTGTTAAATAACTAAGGTCTTTGTTATTTAATTTTATTTTTAAAATGATCTATTTCATTTTGTCAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATACTGTTAGAAACCGGACAGGAATTCACTTTTACCATACGGAGAGGTGTTAGCACCACAGATTCTGTCAGCGTGAACTATGATGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTATGCTGTTTTTATTAACTTTTTTCCCCTATTTTCTGTTTATATTTTTTTTTGCCACTAATTCGAAGCTTTGTTTAGATAGAATACTGGGTTACATTCCATGTGGTGGAAAGTCTTGGCTTTGTAGCATAGGAATATAGTGTTATATGAGAATGAAAGTCTAGTGTGCATCAGTTCTCTCTTTCTTTACTTTTTTGATATTACTACTTGAAATTTCTATAAAGAAAATTAAGAACCCCTCTGTCATCATCTCGTGTGGCAGCAACATTGACTCTTTTAATTTATTATAGGTGGAATGATGTCGTTGATGGTGAAGTCTAAGACAGAGGATTCTGTCAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGACGACATTTGAATGTTCGAGGGAAAAGTGCAACATTGCCTTCCATTACTGGTTGGACTTTGAATTAACATATTCTATAGATTTTCACTTTTTATTGAAGTTTCATTTTAAGTCGTAATGGATTGATAGGGCGAATGATTTTTCTTCTTTTGCCTGTGACTCAATTAATATGTTGTACTGCTTATGTTTCTTGCAGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCTGTCTCATTTGTTAAAGATGCACAAGTAGTTCATGAGTTGAAAAAATATCTTAAAAGTAAATAGCTGCAGATCTTTATGTTGTGCTTGTAATTTGGATGCTGCATAGAGTTTGGATTTATTAACTTTTCTGACTCTGCAGGCTGTGATGCTGATATTCACGTCATTGTAAAAATAGAAAGTGCGGACTCCATACCAAATTTGCATTCAATTATTACAGCTTCTGATGGGGTAATTATTCGATTTATTTTTTTTCACAGCATCGGATTGGATATTCCCATGTTAATGTTGAATTCTGTTCAAGCATTTTCAATGTTGTTGATAGTCTGCGGCTTGGGGTTGCTAATTTAAGGGGAAATTCCTTTTTCTCATTTTAGGCAATGGTTGCAAGAGGCGATCTTGGTGCAGAACTCCCTATTGAAGAGGTTCCACTTCTGCAGGTAAGTTTTTCCCAATTAAAGAGAAGCAGCAGAGGTTTATTTTCCAGCCATGCTTTTTTCCCACTCATAGAAGAGATCTTTAGTGTTTTATTTGTCAAACTTTTCTAAACAAACATGATTGATATGAAGGAGGAGATAATCAACCTGTGTCGAGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCGACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCAGTTAGAGAGGGGGCTGATGCAATCATGCTCTCTGGAGAAACTGCTCATGGAAAGTGAGTTATCCCTTTTGTTCCTTATTATGCTTTTATTTAACCTGCGATTGGCTCAGACATTTGGGGCACAGTATATTGTTTTTGCAATTTAGGTCCGGATTATGGTGGGTACATCATGATATGATTCTGCAAAACAAAAAATTTGTTTTCGTTAATTGCTTTTGAAAAGCTAGAATACATGATATGCGCAGATATTGAGCGTATTGCATTCAGTTTATATAATAAAAAAGGAATTAAACTAATTTCAAGAAAATAAACACACTTCAGCTTGCTTCTTTTGTTGTCTTGATGTCTATTTGGTGAATAAAATAAATAAAGTATATTCGTTCATTTGATCACCCGATGATGCCAATTTCCAGATTTCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCAACCATAGAAGGTGGTCGAATGCCATTGAATCTTGGTCAAACATTCAAGGTTATTTAAAAATTAGTAAATTTATGGTACTTCAATAAGACCCTGATATGTTGCTCCTCCCCCTCCTGTTCTTTTATATTAGTTCATTTTTTATCATGATACTAACTTGGTTACTTTTCTTCTATGTAGAACCATATGAGTGAGATGTTCGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTCATGGCTATCCTCCTAAGCCATTATCGACCATCTGGGACAACATTTGCCTTTACAAATGAGTGAGTAGCCTTTGTACCCTTTTTGATGATAGAGTTGATTGTTAATTGAAAGCCAATCATACACTATGTGACCTAGATATGTTTGACAGAATCTAAACTTGTGTTTATCGAACTGCTTCATCTCCCTTTCCTTATCATTTATGTGTCTGATCATTCCTTCTATTATACTCAAAGTACGAGCATAAGGGCTTTGGTGGGTTTGATCAAGTCAAAATCATATACAATGTACAAACATTAAGTTCAATGTTGATGAAAATGTTTGCAATTTCAATATTGATCCACTGAGGTTGTTAAACCACCCCTAGGATCCATAAAAAGGGCCAGTATCATGGATGAAAATGCTTCCTTTCCTGCATGTGATCAATTGAGTTATCCATGAAAAGAAATAACAATTTAGTTAGGTACTAGGATTTCAGCTATGGTAGTTTCCTTAAATACCCTGCCGTGGCATGCAGCAAAACGATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGCAGACGTTTGCTGATGCCTTATCTCTGCTGCAGGTATCGTATCTGCCAATCTGTTTTATTTTTTCCCTTTTCTTTTCTTCAGGAACTTTTTAATGGCTTCTCACTATAAAACCATGGGTTGACCTAGTCAGTAAGGGTTAGAGGGAATGAGTTAAAGTCATGGTGGCCATTTACCTAAGATTTAACATCTTACAAGTTATCTTGGCAACCACATGTAGTGGAGTCAAGCGGTTGTCCCATGAGAATAGTCGAAGAGTGCACAAGCTAGCTTGAACACTCGCGTATCAAAAAAAGAAAAGACTTCTCAATATAATCTCTCGTTTCAATGTATGGGAATCTCTTTGACTCATTGAGTTTGTCGTGTACAGAGGCAAGGAATGGTGAAGGAAGGGGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTCCGCAAAGTGTAGAGTAGAGTCCAAAAACGTGAGAAACGCTACTTAATTATTCTGCCTGTGCCTTCATTATTTTTGCTTTTCTCCCCATCAGTATGTGAACCTCAACATTTCCTTATTCATTTTTATCTACGAGTATGAGATGCCTAGCTTGTTTTCTGGGTTATGTAGGTAGCTAGCTGTGATAAATTTTTTAGCTTCTGTAGCTCATAATCTTTTATCAATAATTTTCGTGTAGTGTGTAGTTGATGCAGTTGAAGTACGGCCTCTGACTCTGAACTCACAGCTTCATTATCTTCTTTCAGTTCAATTCAGGGCTTATGATACTTCCTGGTTTGTTTGTGGCCCCTACGGATTCTAGTTGATCTTTTGAAATTCTTATCTGAAAGAAAATTTGGTTTAATCATAGGGTTCTTATTCCTCAACTACCAGACATTATTAGTCGTTATGATGGCAGCTCATTAGTTAAAGGCTCAGCAAGCCAGCAATCTTTCCGTAGTTATTAAGGGGCCGTTTCGTAGTTATTAAGGGGCCATTTGGTGCAAGAATTTTGGGATAAGAATGGGTAAGGGTTTTGCATACCCTTGTTTGGTAGAAG

mRNA sequence

TGAACGAAGCCTAACCGCGCTACTAGCTTCCCATTTCTTCGATACAACCGAAGCTCTCTCTCACTCACACATTGCTAACTCCGCCTGAGGATTCTGGCGAAATTTTCTACGTTCAACTCATCAAGCATTTCTCCGTTCGTTTCGCCCTCTGTTAAATCTCTTCCTCGGGAGCTTTCTTGTTCAGTTTCTCTCTCTGTTTTTGCTTCTGTTCGGAAAATGTCTCAGGTGGTGGCTACGAGGTCGATTCACAATACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGCTGGACAGAGCTCGGTCTTTTGGTTTTGGCTCCAAAGTGTTGGCGCATGAGAAGAAGAGTTGGAATTTTGTTTGCAGGAAAAGTCTAATCACATCCAAGAGAGCTGCTCAAGCTGAAGTTGTTCCGGTATCACCTGAGGATGTTCAGAAGGCAGACGAGCAATTTGAACATTTACGAGCAGTTCAACAACTCGGCGATACACCTGTTGGAATGTGGTCAAAACCAACAGTTAGACGCAAGACAAAAATCGTGTGCACAATTGGACCTTCTACTAACACGAAAGAAATGATTTGGAAACTGGCTGAGGCTGGAATGAATGTTGCCCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGGGAATACAATGCCCAATCACAGGACAACTGCATTGCAATCATGCTTGACACCAAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATACTGTTAGAAACCGGACAGGAATTCACTTTTACCATACGGAGAGGTGTTAGCACCACAGATTCTGTCAGCGTGAACTATGATGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTGGAATGATGTCGTTGATGGTGAAGTCTAAGACAGAGGATTCTGTCAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGACGACATTTGAATGTTCGAGGGAAAAGTGCAACATTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCTGTCTCATTTGTTAAAGATGCACAAGTAGTTCATGAGTTGAAAAAATATCTTAAAAGCTGTGATGCTGATATTCACGTCATTGTAAAAATAGAAAGTGCGGACTCCATACCAAATTTGCATTCAATTATTACAGCTTCTGATGGGGCAATGGTTGCAAGAGGCGATCTTGGTGCAGAACTCCCTATTGAAGAGGTTCCACTTCTGCAGGAGGAGATAATCAACCTGTGTCGAGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCGACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCAGTTAGAGAGGGGGCTGATGCAATCATGCTCTCTGGAGAAACTGCTCATGGAAAATTTCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCAACCATAGAAGGTGGTCGAATGCCATTGAATCTTGGTCAAACATTCAAGAACCATATGAGTGAGATGTTCGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTCATGGCTATCCTCCTAAGCCATTATCGACCATCTGGGACAACATTTGCCTTTACAAATGACAAAACGATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGCAGACGTTTGCTGATGCCTTATCTCTGCTGCAGAGGCAAGGAATGGTGAAGGAAGGGGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTCCGCAAAGTGTAGAGTAGAGTCCAAAAACTGTGTAGTTGATGCAGTTGAAGTACGGCCTCTGACTCTGAACTCACAGCTTCATTATCTTCTTTCAGTTCAATTCAGGGCTTATGATACTTCCTGGTTTGTTTGTGGCCCCTACGGATTCTAGTTGATCTTTTGAAATTCTTATCTGAAAGAAAATTTGGTTTAATCATAGGGTTCTTATTCCTCAACTACCAGACATTATTAGTCGTTATGATGGCAGCTCATTAGTTAAAGGCTCAGCAAGCCAGCAATCTTTCCGTAGTTATTAAGGGGCCGTTTCGTAGTTATTAAGGGGCCATTTGGTGCAAGAATTTTGGGATAAGAATGGGTAAGGGTTTTGCATACCCTTGTTTGGTAGAAG

Coding sequence (CDS)

ATGTCTCAGGTGGTGGCTACGAGGTCGATTCACAATACATTCCTGTGCCCTAGCTCGGGATCTGTGCAGGACCGGCTGGACAGAGCTCGGTCTTTTGGTTTTGGCTCCAAAGTGTTGGCGCATGAGAAGAAGAGTTGGAATTTTGTTTGCAGGAAAAGTCTAATCACATCCAAGAGAGCTGCTCAAGCTGAAGTTGTTCCGGTATCACCTGAGGATGTTCAGAAGGCAGACGAGCAATTTGAACATTTACGAGCAGTTCAACAACTCGGCGATACACCTGTTGGAATGTGGTCAAAACCAACAGTTAGACGCAAGACAAAAATCGTGTGCACAATTGGACCTTCTACTAACACGAAAGAAATGATTTGGAAACTGGCTGAGGCTGGAATGAATGTTGCCCGATTGAATATGTCTCATGGAGACCATGCATCTCATCAGAAAGTTATAGATTTAGTCAGGGAATACAATGCCCAATCACAGGACAACTGCATTGCAATCATGCTTGACACCAAGGGTCCTGAGGTTAGGAGTGGTGACCTACCACAACCAATACTGTTAGAAACCGGACAGGAATTCACTTTTACCATACGGAGAGGTGTTAGCACCACAGATTCTGTCAGCGTGAACTATGATGATTTTGTTAATGATGTAGAAGTTGGGGACATGCTTCTTGTTGATGGTGGAATGATGTCGTTGATGGTGAAGTCTAAGACAGAGGATTCTGTCAAGTGTGAAGTTGTTGATGGAGGAGAGCTCAAGTCCAGACGACATTTGAATGTTCGAGGGAAAAGTGCAACATTGCCTTCCATTACTGAGAAGGACTGGGATGACATAAAATTTGGAGTTGACAACAAAGTTGATTTTTATGCTGTCTCATTTGTTAAAGATGCACAAGTAGTTCATGAGTTGAAAAAATATCTTAAAAGCTGTGATGCTGATATTCACGTCATTGTAAAAATAGAAAGTGCGGACTCCATACCAAATTTGCATTCAATTATTACAGCTTCTGATGGGGCAATGGTTGCAAGAGGCGATCTTGGTGCAGAACTCCCTATTGAAGAGGTTCCACTTCTGCAGGAGGAGATAATCAACCTGTGTCGAGGCATGGGGAAGGCTGTCATTGTGGCAACTAACATGCTTGAGAGTATGATTGTTCATCCGACACCAACCAGAGCAGAAGTATCTGACATTGCTATTGCAGTTAGAGAGGGGGCTGATGCAATCATGCTCTCTGGAGAAACTGCTCATGGAAAATTTCCATTAAAAGCTGTGAAAGTAATGCATACAGTTGCTTTACGGACTGAAGCAACCATAGAAGGTGGTCGAATGCCATTGAATCTTGGTCAAACATTCAAGAACCATATGAGTGAGATGTTCGCTTATCATGCAACAATGATGTCCAACACTCTTGGAACCTCAATAGTTGTCTTCACTAGAACTGGTTTCATGGCTATCCTCCTAAGCCATTATCGACCATCTGGGACAACATTTGCCTTTACAAATGACAAAACGATACAACAGAGGTTGGCTTTATATCAGGGAGTGTGCCCCATATACATGCAGTTCTCTGATGATGCTGAGCAGACGTTTGCTGATGCCTTATCTCTGCTGCAGAGGCAAGGAATGGTGAAGGAAGGGGAAGAGGTAGCACTCCTTCAGAGTGGCAGACAACCCATATGGCGGTTCCCATCTACTCACAATATTCAAGTCCGCAAAGTGTAG

Protein sequence

MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRAAQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Homology
BLAST of Tan0001359 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 2.5e-266
Identity = 470/579 (81.17%), Postives = 513/579 (88.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDR-ARSFGFGSKVLAHEKKSWNFV---CRKSLIT 60
           M+QVVATRSI  + L P+ GSV  R ++  +   F  KVL +E K    V    R+ + T
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  SKRAA--QAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
           + R+A  + EV+PVSPEDV   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPE 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ++DN IAIMLDTKGPE
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPE 180

Query: 181 VRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMV 240
           VRSGDLPQPI+L+ GQEFTFTI RGVST   VSVNYDDFVNDVE GDMLLVDGGMMS MV
Sbjct: 181 VRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMV 240

Query: 241 KSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFV 300
           KSKT+DSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW+DIKFGV+NKVDFYAVSFV
Sbjct: 241 KSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFV 300

Query: 301 KDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360
           KDAQVVHELKKYL++  ADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE
Sbjct: 301 KDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360

Query: 361 VPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGET 420
           VP+LQEEIINLCR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGET
Sbjct: 361 VPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420

Query: 421 AHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSI 480
           AHGKFPLKA  VMHTVALRTEATI  G MP NLGQ FKNHMSEMFAYHATMMSNTLGTS 
Sbjct: 421 AHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTST 480

Query: 481 VVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADAL 540
           VVFTRTGFMAILLSHYRPSGT +AFTN+K IQQRLALYQGVCPIYM+F+DDAE+TFA+AL
Sbjct: 481 VVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANAL 540

Query: 541 SLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           + L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 ATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Tan0001359 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 3.9e-211
Identity = 366/470 (77.87%), Postives = 418/470 (88.94%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           RKTKIVCTIGPST+++EMIWKLAEAGMNVARLNMSHGDHASHQ+ IDLV+EYNAQ +D  
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 164 IAIMLDTKGPEVRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDML 223
           IAIMLDTKGPEV SGD+P+PILL+ GQEF F+I+RGVST D+VSVNYDDF+NDVE GD+L
Sbjct: 151 IAIMLDTKGPEVISGDVPKPILLKEGQEFNFSIKRGVSTEDTVSVNYDDFINDVEAGDIL 210

Query: 224 LVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVD 283
           LVDGGMMSL VKSKT D VKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV+
Sbjct: 211 LVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVN 270

Query: 284 NKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVAR 343
           N+VDFYAVSFVKDA+VVHELK YLKSC+ADIHVIVKIESADSIPNLHSII+ASDGAMVAR
Sbjct: 271 NQVDFYAVSFVKDAKVVHELKDYLKSCNADIHVIVKIESADSIPNLHSIISASDGAMVAR 330

Query: 344 GDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVRE 403
           GDLGAELPIEEVPLLQE+II  C+ M K VIVATNMLESMI HPTPTRAEVSDI+IAVRE
Sbjct: 331 GDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAEVSDISIAVRE 390

Query: 404 GADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHA 463
           GADA+MLSGETAHGK+PLKAVKVMH VALRTE++++      +    +K+HM EMFA+H+
Sbjct: 391 GADAVMLSGETAHGKYPLKAVKVMHIVALRTESSLQKSTSSPSQSAAYKSHMGEMFAFHS 450

Query: 464 TMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFS 523
           + M+NTL T I+VFTRTG MAI+LSH RPS T FAFTN++ ++QRLALY GV PIYM+FS
Sbjct: 451 SSMANTLSTPIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQRLALYHGVVPIYMEFS 510

Query: 524 DDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
            DAE+TF+ A+ LL  + +VK+G+ V L+QSG QPIWR  STH+IQVRKV
Sbjct: 511 SDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHSTHHIQVRKV 560

BLAST of Tan0001359 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 4.1e-208
Identity = 369/471 (78.34%), Postives = 410/471 (87.05%), Query Frame = 0

Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKGPEVRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDM 222
            IAIMLDTKGPEVRSGD+PQPI LE GQEF FTI+RGVS  D+VSVNYDDFVNDVEVGD+
Sbjct: 158 AIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDI 217

Query: 223 LLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV 282
           LLVDGGMMSL VKSKT D VKC V+DGGEL+SRRHLNVRGKSATLPSIT+KDW+DIKFGV
Sbjct: 218 LLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGV 277

Query: 283 DNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVA 342
           DN+VDFYAVSFVKDA+VVHELK YLK+C ADI VIVKIESADSI NL SII+A DGAMVA
Sbjct: 278 DNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVA 337

Query: 343 RGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVR 402
           RGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESMI HPTPTRAEVSDIAIAVR
Sbjct: 338 RGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVR 397

Query: 403 EGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYH 462
           EGADAIMLSGETAHGKFPLKAV VMHTVALRTEA++   R   +    +K HM +MFA+H
Sbjct: 398 EGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRTSASRTTAYKGHMGQMFAFH 457

Query: 463 ATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQF 522
           A++M+NTL + ++VFTRTG MA+LLSHYRPS T FAFTN + I QRLALYQGV PIYM+F
Sbjct: 458 ASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEF 517

Query: 523 SDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           SDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  STH IQVRK+
Sbjct: 518 SDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKI 567

BLAST of Tan0001359 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.5e-181
Identity = 326/418 (77.99%), Postives = 365/418 (87.32%), Query Frame = 0

Query: 156 NAQSQDNCIAIMLDTKGPEVRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVN 215
           NAQS DN ++IMLDTKGPEVRSGD+PQP+L E GQEF  TIRRGVST D+VSVNYDDFVN
Sbjct: 1   NAQSHDNVVSIMLDTKGPEVRSGDVPQPMLKE-GQEFNPTIRRGVSTQDTVSVNYDDFVN 60

Query: 216 DVEVGDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW 275
           DV VGD+LLVDGGMMSL VKSKT D VKC VVDGGELKSRRHLNVRGKSA LPSIT+KDW
Sbjct: 61  DVVVGDILLVDGGMMSLAVKSKTSDLVKCVVVDGGELKSRRHLNVRGKSARLPSITDKDW 120

Query: 276 DDIKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITA 335
            DIKFGVDN+VDFYAVSFVKDA+VVHELK+YLK C+ADIHVIVKIESADSIPNLHSII+A
Sbjct: 121 GDIKFGVDNQVDFYAVSFVKDAKVVHELKEYLKRCNADIHVIVKIESADSIPNLHSIISA 180

Query: 336 SDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVS 395
           SDGAMVARGDLGAELPIEEVPLLQE+II  C  M K VIVATNMLESMI HPTPTRAEVS
Sbjct: 181 SDGAMVARGDLGAELPIEEVPLLQEDIIRRCHSMQKPVIVATNMLESMINHPTPTRAEVS 240

Query: 396 DIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHM 455
           DIAIAVREGADA+MLSGETAHGK+PLKAV+VMHTVALRTE++      P   G  +K HM
Sbjct: 241 DIAIAVREGADAVMLSGETAHGKYPLKAVRVMHTVALRTESSSPVNTTPPAQG-AYKGHM 300

Query: 456 SEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGV 515
            EMFA+HAT+M+NTL T I+VFTRTG MA+LLSHY+P+ T FAFTN++ I+QRL+LY+GV
Sbjct: 301 GEMFAFHATIMANTLNTPIIVFTRTGSMAVLLSHYQPASTIFAFTNEERIKQRLSLYRGV 360

Query: 516 CPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
            PIYM+FS DAE+TF+ AL LL  +G++ EGE V L+QSG QPIWR  STH+IQVRKV
Sbjct: 361 MPIYMEFSSDAEETFSRALQLLLNKGLLVEGEHVTLVQSGAQPIWRQESTHHIQVRKV 416

BLAST of Tan0001359 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 7.9e-103
Identity = 219/476 (46.01%), Postives = 301/476 (63.24%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           R+TK+VCTIGP+T   E +  LA  GMNVAR+NM HG    H+ VI+ VR  N + +   
Sbjct: 104 RRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLN-EEKGFA 163

Query: 164 IAIMLDTKGPEVRSGDL--PQPILLETGQEFTFTIRRGVSTTD--SVSVNYDDFVNDVEV 223
           +AIM+DT+G E+  GDL        E G+ +TF++R   S     +++VNYD F  DV+V
Sbjct: 164 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKV 223

Query: 224 GDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 283
           GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 224 GDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISS 283

Query: 284 KDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYL--KSCDADIHVIVKIESADSIPNLH 343
           KDW DI FG+   VDF A+SFVK A+V++ LK Y+  +S D+DI VI KIES DS+ NL 
Sbjct: 284 KDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLE 343

Query: 344 SIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPT 403
            II ASDGAMVARGDLGA++P+E+VP  Q+ I+ +CR + K VIVA+ +LESMI +PTPT
Sbjct: 344 EIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPT 403

Query: 404 RAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVAL------RTEATIEGGRMP 463
           RAEV+D++ AVR+ ADA+MLSGE+A G++P KA+ V+ +V++      R E   E   +P
Sbjct: 404 RAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELP 463

Query: 464 LNLGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTRTGFMAILLSHYRPSGTTFAFTNDK 523
             +G T+ + +SE     A  M+N LG  ++ V+T+ G MA LLS  RP    FAFT   
Sbjct: 464 A-IGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTT 523

Query: 524 TIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPI 560
           ++++RL L  G+ P  + F+DD E       SLL+ +GM+K G+ V  +    Q I
Sbjct: 524 SVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSI 577

BLAST of Tan0001359 vs. NCBI nr
Match: XP_008439317.1 (PREDICTED: plastidial pyruvate kinase 2 [Cucumis melo])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 558/573 (97.38%), Postives = 566/573 (98.78%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSVQ+RLDRARSFG GSKVLAHEKKSWN   R+SLITSKRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTFTIRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAEQTF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. NCBI nr
Match: TYK23021.1 (plastidial pyruvate kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 557/573 (97.21%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSVQ+RLDRARSFG GSKVLAHEKKSWN   R+SLITSKRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTFTIRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
            KAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 FKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAEQTF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. NCBI nr
Match: XP_038874991.1 (plastidial pyruvate kinase 2 [Benincasa hispida])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 556/573 (97.03%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSI++TFLCPSSGSVQ+R+DRAR FG GSKV AHEKKSWNFV R+SLITSKRA
Sbjct: 1   MSQVVATRSINSTFLCPSSGSVQERVDRARLFGIGSKVFAHEKKSWNFVYRRSLITSKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDVQKADEQFEH+RAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHIRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTFTIRRGVST DSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFSDDAEQTF DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFTDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. NCBI nr
Match: XP_022140989.1 (plastidial pyruvate kinase 2 [Momordica charantia])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 555/573 (96.86%), Postives = 566/573 (98.78%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSI +TFLCPSSGSVQDR D+ARSFGFGSKVLAHEKKSWNFV R+SL+T+KRA
Sbjct: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTF+IRRGVST DSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAE+TFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. NCBI nr
Match: XP_022922876.1 (plastidial pyruvate kinase 2-like [Cucurbita moschata])

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 553/573 (96.51%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSVQDR+DRARSFG GSK+L HEKKSWNF  R+SLITSK A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTFTIRRGVST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCP+YMQFSDDAEQTF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPLYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. ExPASy TrEMBL
Match: A0A1S3AY45 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 558/573 (97.38%), Postives = 566/573 (98.78%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSVQ+RLDRARSFG GSKVLAHEKKSWN   R+SLITSKRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTFTIRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAEQTF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. ExPASy TrEMBL
Match: A0A5D3DH87 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001310 PE=3 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 557/573 (97.21%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSVQ+RLDRARSFG GSKVLAHEKKSWN   R+SLITSKRA
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQERLDRARSFGTGSKVLAHEKKSWNLSYRRSLITSKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTFTIRRGVST D+VSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFTIRRGVSTADTVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEV+DGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVIDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
            KAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 FKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFS+DAEQTF DAL++LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSEDAEQTFTDALTMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. ExPASy TrEMBL
Match: A0A6J1CHB6 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111011509 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 555/573 (96.86%), Postives = 566/573 (98.78%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSI +TFLCPSSGSVQDR D+ARSFGFGSKVLAHEKKSWNFV R+SL+T+KRA
Sbjct: 1   MSQVVATRSICSTFLCPSSGSVQDRADKARSFGFGSKVLAHEKKSWNFVSRRSLVTAKRA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
           AQA VVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE
Sbjct: 61  AQAAVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLETGQEFTF+IRRGVST DSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLETGQEFTFSIRRGVSTPDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELK YLKSCDADIH+IVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKNYLKSCDADIHIIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIIN+CRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGETAHGKFP
Sbjct: 361 EIINMCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATI GG+MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIAGGQMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAE+TFADALSLLQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEKTFADALSLLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. ExPASy TrEMBL
Match: A0A6J1E5B9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1)

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 553/573 (96.51%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSVQDR+DRARSFG GSK+L HEKKSWNF  R+SLITSK A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVQDRVDRARSFGIGSKLLGHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTFTIRRGVST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCP+YMQFSDDAEQTF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPLYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. ExPASy TrEMBL
Match: A0A6J1J4F4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1)

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 553/573 (96.51%), Postives = 564/573 (98.43%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDRARSFGFGSKVLAHEKKSWNFVCRKSLITSKRA 60
           MSQVVATRSIH+TFLCPSSGSV DR+DRARSFG GSK+L HEKKSWNF  R+SLITSK A
Sbjct: 1   MSQVVATRSIHSTFLCPSSGSVHDRVDRARSFGIGSKLLRHEKKSWNFAYRRSLITSKGA 60

Query: 61  AQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTNTKE 120
            QAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPST+TKE
Sbjct: 61  TQAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGPSTDTKE 120

Query: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180
           MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL
Sbjct: 121 MIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPEVRSGDL 180

Query: 181 PQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMVKSKTED 240
           PQPILLE+GQEFTFTIRRGVST DSVSVNYDDFVNDVEVGDM+LVDGGMMSLMVKSKTED
Sbjct: 181 PQPILLESGQEFTFTIRRGVSTADSVSVNYDDFVNDVEVGDMILVDGGMMSLMVKSKTED 240

Query: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300
           SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV
Sbjct: 241 SVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFVKDAQVV 300

Query: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360
           HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE
Sbjct: 301 HELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPLLQE 360

Query: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420
           EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP
Sbjct: 361 EIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFP 420

Query: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480
           LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT
Sbjct: 421 LKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRT 480

Query: 481 GFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQ 540
           GFMAILLSHYRPSGTTFAFTNDK IQQRLALYQGVCPIYMQFSDDAEQTF+DALS+LQRQ
Sbjct: 481 GFMAILLSHYRPSGTTFAFTNDKRIQQRLALYQGVCPIYMQFSDDAEQTFSDALSMLQRQ 540

Query: 541 GMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           GMVKEGEEVAL+QSG+QPIWRFPSTHNIQVRKV
Sbjct: 541 GMVKEGEEVALVQSGKQPIWRFPSTHNIQVRKV 573

BLAST of Tan0001359 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 919.1 bits (2374), Expect = 1.8e-267
Identity = 470/579 (81.17%), Postives = 513/579 (88.60%), Query Frame = 0

Query: 1   MSQVVATRSIHNTFLCPSSGSVQDRLDR-ARSFGFGSKVLAHEKKSWNFV---CRKSLIT 60
           M+QVVATRSI  + L P+ GSV  R ++  +   F  KVL +E K    V    R+ + T
Sbjct: 1   MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDT 60

Query: 61  SKRAA--QAEVVPVSPEDVQKADEQFEHLRAVQQLGDTPVGMWSKPTVRRKTKIVCTIGP 120
           + R+A  + EV+PVSPEDV   +EQ E L  +QQ GDT VGMWSKPTVRRKTKIVCT+GP
Sbjct: 61  TVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGP 120

Query: 121 STNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNCIAIMLDTKGPE 180
           STNT+EMIWKLAEAGMNVAR+NMSHGDHASH+KVIDLV+EYNAQ++DN IAIMLDTKGPE
Sbjct: 121 STNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPE 180

Query: 181 VRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDMLLVDGGMMSLMV 240
           VRSGDLPQPI+L+ GQEFTFTI RGVST   VSVNYDDFVNDVE GDMLLVDGGMMS MV
Sbjct: 181 VRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMV 240

Query: 241 KSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGVDNKVDFYAVSFV 300
           KSKT+DSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDW+DIKFGV+NKVDFYAVSFV
Sbjct: 241 KSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFV 300

Query: 301 KDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360
           KDAQVVHELKKYL++  ADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE
Sbjct: 301 KDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEE 360

Query: 361 VPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAIMLSGET 420
           VP+LQEEIINLCR MGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADA+MLSGET
Sbjct: 361 VPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGET 420

Query: 421 AHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYHATMMSNTLGTSI 480
           AHGKFPLKA  VMHTVALRTEATI  G MP NLGQ FKNHMSEMFAYHATMMSNTLGTS 
Sbjct: 421 AHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTST 480

Query: 481 VVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQFSDDAEQTFADAL 540
           VVFTRTGFMAILLSHYRPSGT +AFTN+K IQQRLALYQGVCPIYM+F+DDAE+TFA+AL
Sbjct: 481 VVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANAL 540

Query: 541 SLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           + L +QGMVK+GEE+A++QSG QPIWR  STHNIQVRKV
Sbjct: 541 ATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Tan0001359 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 725.7 bits (1872), Expect = 2.9e-209
Identity = 369/471 (78.34%), Postives = 410/471 (87.05%), Query Frame = 0

Query: 103 RRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDN 162
           RRKTKIVCTIGPS++++EMIWKLAEAGMNVARLNMSHGDHASHQ  IDLV+EYN+   D 
Sbjct: 98  RRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK 157

Query: 163 CIAIMLDTKGPEVRSGDLPQPILLETGQEFTFTIRRGVSTTDSVSVNYDDFVNDVEVGDM 222
            IAIMLDTKGPEVRSGD+PQPI LE GQEF FTI+RGVS  D+VSVNYDDFVNDVEVGD+
Sbjct: 158 AIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDI 217

Query: 223 LLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWDDIKFGV 282
           LLVDGGMMSL VKSKT D VKC V+DGGEL+SRRHLNVRGKSATLPSIT+KDW+DIKFGV
Sbjct: 218 LLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGV 277

Query: 283 DNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPNLHSIITASDGAMVA 342
           DN+VDFYAVSFVKDA+VVHELK YLK+C ADI VIVKIESADSI NL SII+A DGAMVA
Sbjct: 278 DNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVA 337

Query: 343 RGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVR 402
           RGDLGAELPIEEVPLLQEEII  CR + K VIVATNMLESMI HPTPTRAEVSDIAIAVR
Sbjct: 338 RGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVR 397

Query: 403 EGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGRMPLNLGQTFKNHMSEMFAYH 462
           EGADAIMLSGETAHGKFPLKAV VMHTVALRTEA++   R   +    +K HM +MFA+H
Sbjct: 398 EGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLP-VRTSASRTTAYKGHMGQMFAFH 457

Query: 463 ATMMSNTLGTSIVVFTRTGFMAILLSHYRPSGTTFAFTNDKTIQQRLALYQGVCPIYMQF 522
           A++M+NTL + ++VFTRTG MA+LLSHYRPS T FAFTN + I QRLALYQGV PIYM+F
Sbjct: 458 ASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEF 517

Query: 523 SDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPIWRFPSTHNIQVRKV 574
           SDDAE T+A +L LLQ + M+KEG+ V L+QSG QPIWR  STH IQVRK+
Sbjct: 518 SDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKI 567

BLAST of Tan0001359 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 375.2 bits (962), Expect = 9.6e-104
Identity = 222/475 (46.74%), Postives = 299/475 (62.95%), Query Frame = 0

Query: 104 RKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVREYNAQSQDNC 163
           R+TK++CTIGP+T   E +  LA  GMNVARLNM HG    H+ VI  VR  N + +   
Sbjct: 117 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLN-EEKGFA 176

Query: 164 IAIMLDTKGPEVRSGDL--PQPILLETGQEFTFTIRRGVSTTD--SVSVNYDDFVNDVEV 223
           +AIM+DT+G E+  GDL        E G+ +TFT+R   S+    ++SV+YD F  DV V
Sbjct: 177 VAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRV 236

Query: 224 GDMLLVDGGMMSLMVKSKTEDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 283
           GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 237 GDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISS 296

Query: 284 KDWDDIKFGVDNKVDFYAVSFVKDAQVVHELKKYL--KSCDADIHVIVKIESADSIPNLH 343
           KDW DI FG+   VDF AVSFVK A+V++ LK YL  +S   +I VI KIES DS+ NL 
Sbjct: 297 KDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLE 356

Query: 344 SIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPTPT 403
            II ASDGAMVARGDLGA++P+E+VP  Q+ I+ +CR + K VIVA+ +LESMI +PTPT
Sbjct: 357 EIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPT 416

Query: 404 RAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGGR----MPLN 463
           RAEV+D++ AVR+ +DA+MLSGE+A G+FP KA+ V+ TV+LR E      +    +PL 
Sbjct: 417 RAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQ 476

Query: 464 -LGQTFKNHMSEMFAYHATMMSNTLGT-SIVVFTRTGFMAILLSHYRPSGTTFAFTNDKT 523
            +G +F + +SE     A  M+N LG  ++ V+T +G MA L+S  RP    FAFT   +
Sbjct: 477 AIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTS 536

Query: 524 IQQRLALYQGVCPIYMQFSDDAEQTFADALSLLQRQGMVKEGEEVALLQSGRQPI 560
           +++RL L  G+ P  + FSDD E       SLL+ +GM+K G+ V  +    Q I
Sbjct: 537 VRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSI 590

BLAST of Tan0001359 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 7.9e-74
Identity = 175/477 (36.69%), Postives = 268/477 (56.18%), Query Frame = 0

Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKIVCT+GP++ + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRT 66

Query: 155 YNAQSQDNCIAIMLDTKGPEVRSGDLPQ--PILLETGQEFTFTIRRGV-STTDSVSVNYD 214
               +   C A+MLDTK P +R+G L +  PI L+ GQE T +I   +   ++++S++Y 
Sbjct: 67  AMDNTGILC-AVMLDTKSPVIRTGFLKEGKPIQLKQGQEITISIDYKIQGDSNTISMSYK 126

Query: 215 DFVNDVEVGDMLLVDGGMMSLMVKS--KTEDSVKCEVVDGGELKSRRHLNVRGKSATLPS 274
               D++ GD++L   G +SL V S  K    V+C   +   L  R+++N+ G    LP+
Sbjct: 127 KLAEDLKPGDVILCSDGTISLNVLSCDKYLGLVRCRCENSALLGERKNVNLPGIVVDLPT 186

Query: 275 ITEKDWDDI-KFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADSIPN 334
           +TEKD +DI ++GV NK+D  A+SFV+    + +++K L      I ++ K+E+ + + N
Sbjct: 187 LTEKDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLLGEHSKSIMLMSKVENQEGVMN 246

Query: 335 LHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIVHPT 394
              I+  SD  MVARGDLG E+PIE++ L Q+ +IN     GK V+ AT MLESM V P 
Sbjct: 247 FDKILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAHGKPVVTATQMLESMTVSPR 306

Query: 395 PTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGG---RMPL 454
           PTRAE +D+A AV +G D +MLSGETA G  P  AV  M  +    E  I+     +  L
Sbjct: 307 PTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDILHKKTL 366

Query: 455 NLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPS-------GTTFA 514
            +     + +  + A   +   +   ++IVV TR G+ A L++ YRPS           A
Sbjct: 367 GMVSLPLSPIESLAASAVSTARSVFASAIVVLTRGGYTAELVAKYRPSVPILSVIMPEIA 426

Query: 515 FTNDKT--IQQRLALYQGVCPIY-MQFSDDAEQTFADALSLLQRQGMVKEGEEVALL 553
             +D    + +R  +Y+G+ P+      D  E+    A+   + +G+ K G+ +  L
Sbjct: 427 ECSDSVAHVARRGLIYRGIIPVVGCSARDSTEEMIRLAIGFAKTKGICKTGDSIVAL 482

BLAST of Tan0001359 vs. TAIR 10
Match: AT3G55650.1 (Pyruvate kinase family protein )

HSP 1 Score: 271.9 bits (694), Expect = 1.1e-72
Identity = 177/493 (35.90%), Postives = 271/493 (54.97%), Query Frame = 0

Query: 95  GMWSKPTVRRKTKIVCTIGPSTNTKEMIWKLAEAGMNVARLNMSHGDHASHQKVIDLVRE 154
           G  +   +R KTKI+CT+GP + + EMI KL +AGMNVAR N SHG H+ HQ+ +D +R 
Sbjct: 7   GQATNGALRSKTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLR- 66

Query: 155 YNAQSQDN---CIAIMLDTKGPEVRSGDLPQ--PILLETGQEFTFTIRRGV-STTDSVSV 214
               + DN     A+MLDTKGPE+R+G L +  PI L  GQE T +I   +   ++ +S+
Sbjct: 67  ---TAMDNTGILSAVMLDTKGPEIRTGFLKEGKPIQLNQGQEITISIDYMIEGDSNVISM 126

Query: 215 NYDDFVNDVEVGDMLLVDGGMMSLMVKS--KTEDSVKCEVVDGGELKSRRHLNVRGKSAT 274
           +Y     DV+ GD++L   G +SL V S  K+   V+C   +   L  R+++N+ G    
Sbjct: 127 SYKKLAEDVKPGDVILCSDGTISLTVLSCDKSFGLVRCRCENSAILGERKNVNLPGIVVD 186

Query: 275 LPSITEKDWDD-IKFGVDNKVDFYAVSFVKDAQVVHELKKYLKSCDADIHVIVKIESADS 334
           LP++TEKD +D I++GV NK+D  A+SFV+    + E+++ L     +I ++ K+E+ + 
Sbjct: 187 LPTLTEKDKEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGEHSKNIMLMSKVENQEG 246

Query: 335 IPNLHSIITASDGAMVARGDLGAELPIEEVPLLQEEIINLCRGMGKAVIVATNMLESMIV 394
           + N   I+  SD  MVARGDLG E+PIE++ L Q+ +I +   +GK V+ AT MLESM V
Sbjct: 247 VMNCEKILENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMANALGKPVVTATQMLESMTV 306

Query: 395 HPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVKVMHTVALRTEATIEGG---R 454
            P PTRAE +D+A AV +G D +MLSGETA G  P  AV  M  +    E  I+     +
Sbjct: 307 SPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPEAAVLTMSRICKEAEDFIDYDILHK 366

Query: 455 MPLNLGQTFKNHMSEMFAYHATMMSNTLGTSIVVFTRTGFMAILLSHYRPS-------GT 514
             L +     + +  + A   +   +   ++IVV T+ G+ A L++ YRPS         
Sbjct: 367 KTLGMVSLPLSPIESLAASVVSTAQSVFASAIVVLTKGGYTAELVAKYRPSVPILSVIVP 426

Query: 515 TFAFTNDKTIQ---------QRLALYQGVCPIYMQFS-------DDAEQTFADALSLLQR 553
             A  ND  +          +R  +Y+ + P+    S       D  E+    A+   + 
Sbjct: 427 EIAQGNDMEMSCSDSVAHAARRGLIYRRIIPVVATGSSARDSNKDATEEMINLAIGFAKT 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLW92.5e-26681.17Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Q405463.9e-21177.87Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q93Z534.1e-20878.34Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
P559642.5e-18177.99Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 ... [more]
Q431177.9e-10346.01Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_008439317.10.0e+0097.38PREDICTED: plastidial pyruvate kinase 2 [Cucumis melo][more]
TYK23021.10.0e+0097.21plastidial pyruvate kinase 2 [Cucumis melo var. makuwa][more]
XP_038874991.10.0e+0097.03plastidial pyruvate kinase 2 [Benincasa hispida][more]
XP_022140989.10.0e+0096.86plastidial pyruvate kinase 2 [Momordica charantia][more]
XP_022922876.10.0e+0096.51plastidial pyruvate kinase 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A1S3AY450.0e+0097.38Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103484139 PE=3 SV=1[more]
A0A5D3DH870.0e+0097.21Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G0013... [more]
A0A6J1CHB60.0e+0096.86Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111011509 PE=3 SV=1[more]
A0A6J1E5B90.0e+0096.51Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430719 PE=3 SV=1[more]
A0A6J1J4F40.0e+0096.51Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482565 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52920.11.8e-26781.17plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.12.9e-20978.34plastidial pyruvate kinase 3 [more]
AT3G22960.19.6e-10446.74Pyruvate kinase family protein [more]
AT3G25960.17.9e-7436.69Pyruvate kinase family protein [more]
AT3G55650.11.1e-7235.90Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 368..392
score: 62.22
coord: 412..428
score: 55.66
coord: 162..178
score: 56.29
coord: 393..411
score: 63.81
coord: 286..300
score: 42.56
coord: 343..367
score: 52.86
coord: 316..342
score: 45.81
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 104..570
e-value: 5.1E-154
score: 511.2
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 36..573
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 446..573
e-value: 1.9E-26
score: 94.1
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 422..573
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 106..431
e-value: 1.4E-131
score: 440.2
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 104..439
e-value: 1.6E-117
score: 392.3
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 174..267
e-value: 1.4E-131
score: 440.2
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 460..553
e-value: 2.4E-14
score: 53.6
NoneNo IPR availablePANTHERPTHR11817:SF2PLASTIDIAL PYRUVATE KINASE 2coord: 36..573
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 314..326
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 95..438
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 173..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001359.1Tan0001359.1mRNA
Tan0001359.2Tan0001359.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity