Tan0001194 (gene) Snake gourd v1

Overview


Name	Tan0001194
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	protein LURP-one-related 5-like
Location	LG04: 85040809 .. 85042026 (+)
RNA-Seq Expression	Tan0001194
Synteny	Tan0001194

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGCTCCGCGTTCATTTTTCAAACCAGACCCACCACTATTACTCTCGCCCATTTCTTCTCTTTTCTAAGCCGTCCCGCCGACCCTTTAACGTCTGTTCCCTTCTCTTTCTCCTCCATTCACTCCCTTTTTATCTTCTTCATAAAAGGACAGAGCTTCCCATTTCTCTGAATCACCAAAAACCCACTAAAATGAAGGTTTCCTCCATTGATGACTCTGAGTTTTCCCGTAATGGAGTCGGCACCGCCGCTACCACCACCACCACCCATCTCACTGTCATGAAAACCTCTCTCTTCTTCGCCGGCGACGGCTTCACTGTCTACGATTGCAAAGGCAAACTCGTCTTTCGAGTCGATTCCTACGGCCCTGATTCTCGTTACAAAGATGAACTCGTTCTAATGGACCCTCATGGCCGTTGCCTCTTCACCGTCCGCCGCAAGGTAATGGCCGATTTCTGTTTCTTTTTCGTTGAATTGAAGTGGGTTTTGGGTAATTTTGGTTTTGGGTTCTTGTTTGTTTGAGTTTTGAGTTATGGGATTTTGACTCTGTTTTTTTTTTTTTGGCGAAAACAGAGACCGAGTTTGCATCAACGGTGGGAAGGGTATCTCCGGGAGAGAATCAACGGTCAGAAACCGATCTTCGGAGTGCGGCGGTCGTCGATCATCGGACGGTCGAGTTTGACGGTGGAGATGTATGGAAATTCCGGCGAGGAGTATCAGATCGAGGGGTCGTTTGGGCAACGTTTATGTAAAGTTTTGAATGCTAAGAAAGAGGCAGTGGCTGAGATTAGGAGGAAAGTTGATGCTTCGACCAATGTTTTGCTTGGAAAAGATGTTCTCTCTCTTTCTGTAAAGCCTGGTTTTAATGGAGCTTTCGCTATGGCTTTGGTGCTTGTTCTTGATAGGATCAACGGTGATGATGGGGCTGGTTCTGGTGATGCACCAGTTCGGACCCTTGATTAATTTTGAGGTTTTTTTTTTTTTTGTTTAAAAGGGGTTGTTTTGTTTGTTTGATTTGGAGCATTGGCTCTTCTTTTTATGTCTTTGTTTGTAGTTTTGAATCTCGATGTGTATATGTAAGTTGTAACCATTTACAGCCTCAAATAGATGGAATTTGAAAATCACCAAACAGAGCTTTAATTGTGGAATGATAGAGAGATACAGTGTTAGAGAATGGAAGAATTTGTTGCATTGCTGAAATGTTGTGTTCAAAATAAAAA

mRNA sequence

TAAGCTCCGCGTTCATTTTTCAAACCAGACCCACCACTATTACTCTCGCCCATTTCTTCTCTTTTCTAAGCCGTCCCGCCGACCCTTTAACGTCTGTTCCCTTCTCTTTCTCCTCCATTCACTCCCTTTTTATCTTCTTCATAAAAGGACAGAGCTTCCCATTTCTCTGAATCACCAAAAACCCACTAAAATGAAGGTTTCCTCCATTGATGACTCTGAGTTTTCCCGTAATGGAGTCGGCACCGCCGCTACCACCACCACCACCCATCTCACTGTCATGAAAACCTCTCTCTTCTTCGCCGGCGACGGCTTCACTGTCTACGATTGCAAAGGCAAACTCGTCTTTCGAGTCGATTCCTACGGCCCTGATTCTCGTTACAAAGATGAACTCGTTCTAATGGACCCTCATGGCCGTTGCCTCTTCACCGTCCGCCGCAAGAGACCGAGTTTGCATCAACGGTGGGAAGGGTATCTCCGGGAGAGAATCAACGGTCAGAAACCGATCTTCGGAGTGCGGCGGTCGTCGATCATCGGACGGTCGAGTTTGACGGTGGAGATGTATGGAAATTCCGGCGAGGAGTATCAGATCGAGGGGTCGTTTGGGCAACGTTTATGTAAAGTTTTGAATGCTAAGAAAGAGGCAGTGGCTGAGATTAGGAGGAAAGTTGATGCTTCGACCAATGTTTTGCTTGGAAAAGATGTTCTCTCTCTTTCTGTAAAGCCTGGTTTTAATGGAGCTTTCGCTATGGCTTTGGTGCTTGTTCTTGATAGGATCAACGGTGATGATGGGGCTGGTTCTGGTGATGCACCAGTTCGGACCCTTGATTAATTTTGAGGTTTTTTTTTTTTTTGTTTAAAAGGGGTTGTTTTGTTTGTTTGATTTGGAGCATTGGCTCTTCTTTTTATGTCTTTGTTTGTAGTTTTGAATCTCGATGTGTATATGTAAGTTGTAACCATTTACAGCCTCAAATAGATGGAATTTGAAAATCACCAAACAGAGCTTTAATTGTGGAATGATAGAGAGATACAGTGTTAGAGAATGGAAGAATTTGTTGCATTGCTGAAATGTTGTGTTCAAAATAAAAA

Coding sequence (CDS)

ATGAAGGTTTCCTCCATTGATGACTCTGAGTTTTCCCGTAATGGAGTCGGCACCGCCGCTACCACCACCACCACCCATCTCACTGTCATGAAAACCTCTCTCTTCTTCGCCGGCGACGGCTTCACTGTCTACGATTGCAAAGGCAAACTCGTCTTTCGAGTCGATTCCTACGGCCCTGATTCTCGTTACAAAGATGAACTCGTTCTAATGGACCCTCATGGCCGTTGCCTCTTCACCGTCCGCCGCAAGAGACCGAGTTTGCATCAACGGTGGGAAGGGTATCTCCGGGAGAGAATCAACGGTCAGAAACCGATCTTCGGAGTGCGGCGGTCGTCGATCATCGGACGGTCGAGTTTGACGGTGGAGATGTATGGAAATTCCGGCGAGGAGTATCAGATCGAGGGGTCGTTTGGGCAACGTTTATGTAAAGTTTTGAATGCTAAGAAAGAGGCAGTGGCTGAGATTAGGAGGAAAGTTGATGCTTCGACCAATGTTTTGCTTGGAAAAGATGTTCTCTCTCTTTCTGTAAAGCCTGGTTTTAATGGAGCTTTCGCTATGGCTTTGGTGCTTGTTCTTGATAGGATCAACGGTGATGATGGGGCTGGTTCTGGTGATGCACCAGTTCGGACCCTTGATTAA

Protein sequence

MKVSSIDDSEFSRNGVGTAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD

Homology

[Close]

BLAST of Tan0001194 vs. ExPASy Swiss-Prot
Match: Q9SSC7 (Protein LURP-one-related 5 OS=Arabidopsis thaliana OX=3702 GN=At1g80120 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 8.9e-68
Identity = 136/185 (73.51%), Postives = 154/185 (83.24%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY-GPDSRYKDELVLMDPHGRCLFTVRRKRP 86
           LTV KTSLFFAGDGFTVYDCKG LVFRVDSY GP++R  DE+VLMD HGRCL T+RRKRP
Sbjct: 19  LTVRKTSLFFAGDGFTVYDCKGSLVFRVDSYGGPNTRDTDEVVLMDAHGRCLLTLRRKRP 78

Query: 87  SLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLTVEMYGN-SGEEYQIEGSFGQRLCKV 146
           SL +RWEGYL ER +GQKPIFGVRRSSIIGR+S+TVE+YG+    EY IEGSFG R C V
Sbjct: 79  SLRRRWEGYLGERSDGQKPIFGVRRSSIIGRNSVTVEVYGDYQCSEYLIEGSFGARNCTV 138

Query: 147 LNAK-KEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGAGS 206
           + A+ +  VAEIRRKVDASTNV+LGKDV SL+VKPGF+GAFAM LVLVLD+I GDD    
Sbjct: 139 VEAETRRKVAEIRRKVDASTNVMLGKDVFSLNVKPGFDGAFAMGLVLVLDQIYGDDLLEV 198

Query: 207 GDAPV 209
           G+  V
Sbjct: 199 GEEQV 203

[Close]

BLAST of Tan0001194 vs. ExPASy Swiss-Prot
Match: Q9LVZ8 (Protein LURP-one-related 12 OS=Arabidopsis thaliana OX=3702 GN=At3g15810 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 5.8e-67
Identity = 121/182 (66.48%), Postives = 146/182 (80.22%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPS 86
           LTV KTSLF+ GDGF  YDC+G ++FRVDSYGPD+R  DE+VLMD  G+CL TV+RKRP+
Sbjct: 33  LTVCKTSLFYTGDGFAAYDCRGDIIFRVDSYGPDTRDNDEIVLMDATGKCLLTVKRKRPT 92

Query: 87  LHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLTVEMYGNSGEEYQIEGSFGQRLCKVLN 146
           LHQRWEG+L ER  GQKPIF VRRSSIIGR ++ VE+Y  +GEEY I+G F QR C + +
Sbjct: 93  LHQRWEGFLGERSEGQKPIFSVRRSSIIGRCTMEVEVYDGTGEEYIIDGDFSQRSCLIYD 152

Query: 147 AKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGAGSGDA 206
            KK  VAEI+RKVDASTNV+LG+DV +L +KPGF+GAFAM LV+VLD+INGDD    GD 
Sbjct: 153 TKKCTVAEIKRKVDASTNVMLGRDVFTLEIKPGFDGAFAMGLVVVLDQINGDDPVEIGDE 212

Query: 207 PV 209
            V
Sbjct: 213 QV 214

[Close]

BLAST of Tan0001194 vs. ExPASy Swiss-Prot
Match: Q9ZVI6 (Protein LURP-one-related 8 OS=Arabidopsis thaliana OX=3702 GN=At2g38640 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.6e-24
Identity = 74/172 (43.02%), Postives = 97/172 (56.40%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPS 86
           LTV K SL F  DGFTVY+  G+LVFRVD+Y    R  D +VLMD  G  L ++RRK+ S
Sbjct: 25  LTVWKKSLLFNCDGFTVYNANGELVFRVDNYMNCPR--DNIVLMDASGFPLLSIRRKKLS 84

Query: 87  LHQRWEGYLRERINGQKPIFGVRRS-SIIGRSSLTVEMYGNSGEEYQIEGSFGQRLCKVL 146
           L   W  Y  E    + PIF  R++ SII        +       Y+IEGS+GQR CK+L
Sbjct: 85  LGDCWMVYDGE--TERDPIFTARKNVSIISNRKSLAWVSAKKTVLYEIEGSYGQRSCKIL 144

Query: 147 NAK--KEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRI 196
           + +  K+  AEI+RK      V  GKDV  L V+       AMAL ++LD++
Sbjct: 145 DERRNKKKTAEIKRKETVIGGVAFGKDVYKLIVESEMEPRVAMALTIILDQM 192

[Close]

BLAST of Tan0001194 vs. ExPASy Swiss-Prot
Match: A0MFL4 (Protein LURP-one-related 17 OS=Arabidopsis thaliana OX=3702 GN=At5g41590 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 3.2e-17
Identity = 65/194 (33.51%), Postives = 100/194 (51.55%), Query Frame = 0


Query: 16  VGTAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGR 75
           +G A TT    LTV + SL  + +GFTV D  G L++RVD+Y       +EL+LMD  G 
Sbjct: 32  IGGACTT----LTVWRKSLLVSCEGFTVIDSNGDLIYRVDNYA--RTRPEELILMDKDGN 91

Query: 76  CLFTVRR-KRPSLHQRWEGYLRERINGQKPI---------FGVRRSSIIGRSSLTVEMYG 135
            L  + R K+ +L   W  Y      G+  I           ++ + +  +S +   +Y 
Sbjct: 92  SLLLMHRTKKITLVDSWGIYEANDTKGETKIPKCPTWYMRKNLKMNILSTKSDILAYVYS 151

Query: 136 NSGEE---YQIEGSFGQRLCKVLNAK-KEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFN 195
            S ++   Y I+GS+  + CK+++    + V EI+RK   +  V  G DV  L V PGF+
Sbjct: 152 GSFDKKNSYIIKGSYRCKSCKIVHVPLNKTVVEIKRKEVRTKGVRFGSDVFDLVVNPGFD 211

[Close]

BLAST of Tan0001194 vs. ExPASy Swiss-Prot
Match: Q9LZX1 (Protein LURP-one-related 15 OS=Arabidopsis thaliana OX=3702 GN=At5g01750 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 50/179 (27.93%), Postives = 95/179 (53.07%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPS 86
           + +++  +      F + D  G L+F+V    P     D+ VL+D  G  + T+R K  S
Sbjct: 40  MAIVRKMMSLTDGNFVITDVNGNLLFKVKE--PVFGLHDKRVLLDGSGTPVVTLREKMVS 99

Query: 87  LHQRWEGYLRERINGQKPIFGVRRSSIIG-RSSLTVEMYGNSGE---EYQIEGSFGQRLC 146
           +H RW+ +     + +  ++ V+RSS++  ++ L V +  N  E   +++++GS+ +R C
Sbjct: 100 MHDRWQVFRGGSTDQRDLLYTVKRSSMLQLKTKLDVFLGHNKDEKRCDFRVKGSWLERSC 159

Query: 147 KVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGA 202
            V   + +A+     +     +V LGKD  S++V P  + AF  +LV++LD +N +D A
Sbjct: 160 VVYAGESDAIVAQMHRKHTVQSVFLGKDNFSVTVYPNVDYAFIASLVVILDDVNREDRA 216

[Close]

BLAST of Tan0001194 vs. NCBI nr
Match: XP_022998678.1 (protein LURP-one-related 5-like [Cucurbita maxima] >XP_022998679.1 protein LURP-one-related 5-like [Cucurbita maxima])

HSP 1 Score: 372.9 bits (956), Expect = 1.9e-99
Identity = 187/216 (86.57%), Postives = 196/216 (90.74%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVG----TAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDS 60
           MKVSSIDDS+F   GVG    TA TTTTTHLTVMKTSLFFAGDGFTVYDC G+LVFRVDS
Sbjct: 1   MKVSSIDDSQFFHKGVGIASSTATTTTTTHLTVMKTSLFFAGDGFTVYDCNGRLVFRVDS 60

Query: 61  YGPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGR 120
           YGPDSRYKDELVLMDPHGRCLFT+RRKRPSLHQRWEGYL ERINGQ+PIFGVRRSS+IGR
Sbjct: 61  YGPDSRYKDELVLMDPHGRCLFTLRRKRPSLHQRWEGYLHERINGQRPIFGVRRSSVIGR 120

Query: 121 SSLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSV 180
           SS+TVEMY NS EEYQIEGSFGQRLCK+LNAKKE VAEI+RKVDASTNVLLGKDV SLSV
Sbjct: 121 SSVTVEMYRNSSEEYQIEGSFGQRLCKILNAKKETVAEIKRKVDASTNVLLGKDVFSLSV 180

Query: 181 KPGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           KPGFNGAFAMALVLVLDRINGDDG  S   P RTL+
Sbjct: 181 KPGFNGAFAMALVLVLDRINGDDGVYSSSEPNRTLN 216

[Close]

BLAST of Tan0001194 vs. NCBI nr
Match: XP_022948684.1 (protein LURP-one-related 5-like [Cucurbita moschata])

HSP 1 Score: 369.4 bits (947), Expect = 2.1e-98
Identity = 186/215 (86.51%), Postives = 195/215 (90.70%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVGTA---ATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY 60
           MKVSSIDDS+F   GVG A   ATTTTTHLTVMKTSLFFAGDGFTVYDC G+LVFRVDSY
Sbjct: 1   MKVSSIDDSKFFHKGVGIASSTATTTTTHLTVMKTSLFFAGDGFTVYDCYGRLVFRVDSY 60

Query: 61  GPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRS 120
           GPDSRYKDELVLMDPHGRCLFT+RRKRPSLHQRWEGYL ERINGQ+PIFGVRRSS+IGRS
Sbjct: 61  GPDSRYKDELVLMDPHGRCLFTLRRKRPSLHQRWEGYLHERINGQRPIFGVRRSSVIGRS 120

Query: 121 SLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVK 180
           S+TVEMY NS EEYQIEGSFGQRLCK+LNAKKE VAEI+RKVDASTNVLLGKDV SL VK
Sbjct: 121 SVTVEMYRNSSEEYQIEGSFGQRLCKILNAKKETVAEIKRKVDASTNVLLGKDVFSLCVK 180

Query: 181 PGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           PGFNGAFAMALVLVLDRINGDDG  S   P RTL+
Sbjct: 181 PGFNGAFAMALVLVLDRINGDDGVYSSSEPNRTLN 215

[Close]

BLAST of Tan0001194 vs. NCBI nr
Match: XP_023523506.1 (protein LURP-one-related 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.0 bits (946), Expect = 2.7e-98
Identity = 186/215 (86.51%), Postives = 195/215 (90.70%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVGTA---ATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY 60
           MKVSSIDDS+F   GVG A   ATTTTTHLTVMKTSLFFAGDGFTVYDC G+LVFRVDSY
Sbjct: 1   MKVSSIDDSKFFHKGVGIASSTATTTTTHLTVMKTSLFFAGDGFTVYDCYGRLVFRVDSY 60

Query: 61  GPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRS 120
           GPDSRYKDELVLMDPHGRCLFT+RRKRPSLHQRWEGYL ERINGQ+PIFGVRRSS+IGRS
Sbjct: 61  GPDSRYKDELVLMDPHGRCLFTLRRKRPSLHQRWEGYLHERINGQRPIFGVRRSSVIGRS 120

Query: 121 SLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVK 180
           S+TVEMY NS EEYQIEGSFGQRLCK+LNAKKE VAEI+RKVDASTNVLLGKDV SL VK
Sbjct: 121 SVTVEMYRNSSEEYQIEGSFGQRLCKILNAKKETVAEIKRKVDASTNVLLGKDVFSLCVK 180

Query: 181 PGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           PGFNGAFAMALVLVLDRINGDDG  S   P RTL+
Sbjct: 181 PGFNGAFAMALVLVLDRINGDDGLYSSSEPNRTLN 215

[Close]

BLAST of Tan0001194 vs. NCBI nr
Match: KAG6606800.1 (Protein LURP-one-related 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 366.3 bits (939), Expect = 1.8e-97
Identity = 183/215 (85.12%), Postives = 195/215 (90.70%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVGTAATT---TTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY 60
           MKVSSIDDS+F   GVG A++T   TTTHLTVMKTSLFFAGDGFTVYDC G+LVFRVDSY
Sbjct: 1   MKVSSIDDSKFFHKGVGIASSTAAATTTHLTVMKTSLFFAGDGFTVYDCYGRLVFRVDSY 60

Query: 61  GPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRS 120
           GPDSRYKDELVLMDPHGRCLFT+RRKRPSLHQRWEGYL ERINGQ+P+FGVRRSS+IGRS
Sbjct: 61  GPDSRYKDELVLMDPHGRCLFTLRRKRPSLHQRWEGYLHERINGQRPVFGVRRSSVIGRS 120

Query: 121 SLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVK 180
           S+TVEMY NS EEYQIEGSFGQRLCK+LNAKKE VAEI+RKVDASTNVLLGKDV SL VK
Sbjct: 121 SVTVEMYRNSSEEYQIEGSFGQRLCKILNAKKETVAEIKRKVDASTNVLLGKDVFSLCVK 180

Query: 181 PGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           PGFNGAFAMALVLVLDRINGDDG  S   P RTL+
Sbjct: 181 PGFNGAFAMALVLVLDRINGDDGVYSSSEPNRTLN 215

[Close]

BLAST of Tan0001194 vs. NCBI nr
Match: XP_023526859.1 (protein LURP-one-related 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 365.5 bits (937), Expect = 3.0e-97
Identity = 181/211 (85.78%), Postives = 195/211 (92.42%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVGTAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPD 60
           MKVSSIDDSEFS NG+G+  TTT TH TVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPD
Sbjct: 1   MKVSSIDDSEFSCNGIGSIPTTTDTHFTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPD 60

Query: 61  SRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLT 120
           +RY+DELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKP+FGVRRSSIIG SSLT
Sbjct: 61  ARYRDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPVFGVRRSSIIGPSSLT 120

Query: 121 VEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGF 180
           VEMYGN G+EYQIEGSFGQRLC VLN KKEAVA+IRRKVDASTNV+LGKDV SLS+KPGF
Sbjct: 121 VEMYGNPGKEYQIEGSFGQRLCTVLNVKKEAVAKIRRKVDASTNVVLGKDVFSLSLKPGF 180

Query: 181 NGAFAMALVLVLDRINGDDGAGSGDAPVRTL 212
           +GAFAMALVL+LD+I+GD+G G    P RTL
Sbjct: 181 DGAFAMALVLILDQISGDEGIGG--EPDRTL 209

[Close]

BLAST of Tan0001194 vs. ExPASy TrEMBL
Match: A0A6J1KEZ1 (protein LURP-one-related 5-like OS=Cucurbita maxima OX=3661 GN=LOC111493264 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 9.1e-100
Identity = 187/216 (86.57%), Postives = 196/216 (90.74%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVG----TAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDS 60
           MKVSSIDDS+F   GVG    TA TTTTTHLTVMKTSLFFAGDGFTVYDC G+LVFRVDS
Sbjct: 1   MKVSSIDDSQFFHKGVGIASSTATTTTTTHLTVMKTSLFFAGDGFTVYDCNGRLVFRVDS 60

Query: 61  YGPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGR 120
           YGPDSRYKDELVLMDPHGRCLFT+RRKRPSLHQRWEGYL ERINGQ+PIFGVRRSS+IGR
Sbjct: 61  YGPDSRYKDELVLMDPHGRCLFTLRRKRPSLHQRWEGYLHERINGQRPIFGVRRSSVIGR 120

Query: 121 SSLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSV 180
           SS+TVEMY NS EEYQIEGSFGQRLCK+LNAKKE VAEI+RKVDASTNVLLGKDV SLSV
Sbjct: 121 SSVTVEMYRNSSEEYQIEGSFGQRLCKILNAKKETVAEIKRKVDASTNVLLGKDVFSLSV 180

Query: 181 KPGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           KPGFNGAFAMALVLVLDRINGDDG  S   P RTL+
Sbjct: 181 KPGFNGAFAMALVLVLDRINGDDGVYSSSEPNRTLN 216

[Close]

BLAST of Tan0001194 vs. ExPASy TrEMBL
Match: A0A6J1GAM5 (protein LURP-one-related 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452281 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.0e-98
Identity = 186/215 (86.51%), Postives = 195/215 (90.70%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVGTA---ATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY 60
           MKVSSIDDS+F   GVG A   ATTTTTHLTVMKTSLFFAGDGFTVYDC G+LVFRVDSY
Sbjct: 1   MKVSSIDDSKFFHKGVGIASSTATTTTTHLTVMKTSLFFAGDGFTVYDCYGRLVFRVDSY 60

Query: 61  GPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRS 120
           GPDSRYKDELVLMDPHGRCLFT+RRKRPSLHQRWEGYL ERINGQ+PIFGVRRSS+IGRS
Sbjct: 61  GPDSRYKDELVLMDPHGRCLFTLRRKRPSLHQRWEGYLHERINGQRPIFGVRRSSVIGRS 120

Query: 121 SLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVK 180
           S+TVEMY NS EEYQIEGSFGQRLCK+LNAKKE VAEI+RKVDASTNVLLGKDV SL VK
Sbjct: 121 SVTVEMYRNSSEEYQIEGSFGQRLCKILNAKKETVAEIKRKVDASTNVLLGKDVFSLCVK 180

Query: 181 PGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           PGFNGAFAMALVLVLDRINGDDG  S   P RTL+
Sbjct: 181 PGFNGAFAMALVLVLDRINGDDGVYSSSEPNRTLN 215

[Close]

BLAST of Tan0001194 vs. ExPASy TrEMBL
Match: A0A6J1GU66 (protein LURP-one-related 5-like OS=Cucurbita moschata OX=3662 GN=LOC111457578 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.6e-96
Identity = 181/214 (84.58%), Postives = 194/214 (90.65%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVG---TAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY 60
           MKVSSIDDSEFS NG+G   T  TTT TH TVMKTSLFFAGDGFTVYDCKGKLVFRVDSY
Sbjct: 1   MKVSSIDDSEFSCNGIGSIPTTTTTTDTHFTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY 60

Query: 61  GPDSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRS 120
           GPD+RY+DELVLMDPHG CLFTVRRKRPSLHQRWEGYLRERINGQKP+FGVRRSSIIG S
Sbjct: 61  GPDARYRDELVLMDPHGHCLFTVRRKRPSLHQRWEGYLRERINGQKPVFGVRRSSIIGPS 120

Query: 121 SLTVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVK 180
           SLTV+MYGN G+EYQIEGSFGQRLC VLN KKEAVA+IRRKVDASTNV+LGKDV SLS+K
Sbjct: 121 SLTVDMYGNPGKEYQIEGSFGQRLCTVLNVKKEAVAKIRRKVDASTNVVLGKDVFSLSLK 180

Query: 181 PGFNGAFAMALVLVLDRINGDDGAGSGDAPVRTL 212
           PGF+GAFAMALVL+LD+I+GDDG G    P RTL
Sbjct: 181 PGFDGAFAMALVLILDQISGDDGIGG--EPDRTL 212

[Close]

BLAST of Tan0001194 vs. ExPASy TrEMBL
Match: A0A0A0KYG2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G005540 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 181/212 (85.38%), Postives = 189/212 (89.15%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNGVGTAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPD 60
           MKVSSIDDS FS  G+  ++TTTTTH TVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPD
Sbjct: 1   MKVSSIDDSLFSCKGLVGSSTTTTTHFTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPD 60

Query: 61  SRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLT 120
           SRY+DELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSS T
Sbjct: 61  SRYRDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSFT 120

Query: 121 VEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGF 180
           VEMYGN GEEYQIEG FGQRLC VLN KKE VA IRRKVDASTNVLLGKDV SLS+ PGF
Sbjct: 121 VEMYGNPGEEYQIEGCFGQRLCTVLNVKKEPVATIRRKVDASTNVLLGKDVFSLSLNPGF 180

Query: 181 NGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           +GAFAMALVLVLDRING +G  S   P + LD
Sbjct: 181 DGAFAMALVLVLDRING-EGVDSSGNPEQILD 211

[Close]

BLAST of Tan0001194 vs. ExPASy TrEMBL
Match: A0A5D3E0H7 (Protein LURP-one-related 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001790 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.0e-94
Identity = 182/213 (85.45%), Postives = 190/213 (89.20%), Query Frame = 0


Query: 1   MKVSSIDDSEFSRNG-VGTAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGP 60
           MKVSSIDDS FS  G VG++ TTTTTH TVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGP
Sbjct: 1   MKVSSIDDSLFSCKGLVGSSTTTTTTHFTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGP 60

Query: 61  DSRYKDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSL 120
           DSRY+DELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSS 
Sbjct: 61  DSRYRDELVLMDPHGRCLFTVRRKRPSLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSF 120

Query: 121 TVEMYGNSGEEYQIEGSFGQRLCKVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPG 180
           TVEMYGN GEEYQIEG FGQRLC VLN +KE VA IRRKVDAST VLLGKDV SLS+ PG
Sbjct: 121 TVEMYGNPGEEYQIEGCFGQRLCTVLNVRKEPVATIRRKVDASTKVLLGKDVFSLSLNPG 180

Query: 181 FNGAFAMALVLVLDRINGDDGAGSGDAPVRTLD 213
           F+GAFAMALVLVLDRING +G  S   P +TLD
Sbjct: 181 FDGAFAMALVLVLDRING-EGVDSSGNPDQTLD 212

[Close]

BLAST of Tan0001194 vs. TAIR 10
Match: AT1G80120.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 258.1 bits (658), Expect = 6.3e-69
Identity = 136/185 (73.51%), Postives = 154/185 (83.24%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSY-GPDSRYKDELVLMDPHGRCLFTVRRKRP 86
           LTV KTSLFFAGDGFTVYDCKG LVFRVDSY GP++R  DE+VLMD HGRCL T+RRKRP
Sbjct: 19  LTVRKTSLFFAGDGFTVYDCKGSLVFRVDSYGGPNTRDTDEVVLMDAHGRCLLTLRRKRP 78

Query: 87  SLHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLTVEMYGN-SGEEYQIEGSFGQRLCKV 146
           SL +RWEGYL ER +GQKPIFGVRRSSIIGR+S+TVE+YG+    EY IEGSFG R C V
Sbjct: 79  SLRRRWEGYLGERSDGQKPIFGVRRSSIIGRNSVTVEVYGDYQCSEYLIEGSFGARNCTV 138

Query: 147 LNAK-KEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGAGS 206
           + A+ +  VAEIRRKVDASTNV+LGKDV SL+VKPGF+GAFAM LVLVLD+I GDD    
Sbjct: 139 VEAETRRKVAEIRRKVDASTNVMLGKDVFSLNVKPGFDGAFAMGLVLVLDQIYGDDLLEV 198

Query: 207 GDAPV 209
           G+  V
Sbjct: 199 GEEQV 203

[Close]

BLAST of Tan0001194 vs. TAIR 10
Match: AT3G15810.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 255.4 bits (651), Expect = 4.1e-68
Identity = 121/182 (66.48%), Postives = 146/182 (80.22%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPS 86
           LTV KTSLF+ GDGF  YDC+G ++FRVDSYGPD+R  DE+VLMD  G+CL TV+RKRP+
Sbjct: 33  LTVCKTSLFYTGDGFAAYDCRGDIIFRVDSYGPDTRDNDEIVLMDATGKCLLTVKRKRPT 92

Query: 87  LHQRWEGYLRERINGQKPIFGVRRSSIIGRSSLTVEMYGNSGEEYQIEGSFGQRLCKVLN 146
           LHQRWEG+L ER  GQKPIF VRRSSIIGR ++ VE+Y  +GEEY I+G F QR C + +
Sbjct: 93  LHQRWEGFLGERSEGQKPIFSVRRSSIIGRCTMEVEVYDGTGEEYIIDGDFSQRSCLIYD 152

Query: 147 AKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGAGSGDA 206
            KK  VAEI+RKVDASTNV+LG+DV +L +KPGF+GAFAM LV+VLD+INGDD    GD 
Sbjct: 153 TKKCTVAEIKRKVDASTNVMLGRDVFTLEIKPGFDGAFAMGLVVVLDQINGDDPVEIGDE 212

Query: 207 PV 209
            V
Sbjct: 213 QV 214

[Close]

BLAST of Tan0001194 vs. TAIR 10
Match: AT2G38640.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 112.8 bits (281), Expect = 3.3e-25
Identity = 74/172 (43.02%), Postives = 97/172 (56.40%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPS 86
           LTV K SL F  DGFTVY+  G+LVFRVD+Y    R  D +VLMD  G  L ++RRK+ S
Sbjct: 25  LTVWKKSLLFNCDGFTVYNANGELVFRVDNYMNCPR--DNIVLMDASGFPLLSIRRKKLS 84

Query: 87  LHQRWEGYLRERINGQKPIFGVRRS-SIIGRSSLTVEMYGNSGEEYQIEGSFGQRLCKVL 146
           L   W  Y  E    + PIF  R++ SII        +       Y+IEGS+GQR CK+L
Sbjct: 85  LGDCWMVYDGE--TERDPIFTARKNVSIISNRKSLAWVSAKKTVLYEIEGSYGQRSCKIL 144

Query: 147 NAK--KEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRI 196
           + +  K+  AEI+RK      V  GKDV  L V+       AMAL ++LD++
Sbjct: 145 DERRNKKKTAEIKRKETVIGGVAFGKDVYKLIVESEMEPRVAMALTIILDQM 192

[Close]

BLAST of Tan0001194 vs. TAIR 10
Match: AT5G41590.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 90.1 bits (222), Expect = 2.3e-18
Identity = 65/194 (33.51%), Postives = 100/194 (51.55%), Query Frame = 0


Query: 16  VGTAATTTTTHLTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGR 75
           +G A TT    LTV + SL  + +GFTV D  G L++RVD+Y       +EL+LMD  G 
Sbjct: 32  IGGACTT----LTVWRKSLLVSCEGFTVIDSNGDLIYRVDNYA--RTRPEELILMDKDGN 91

Query: 76  CLFTVRR-KRPSLHQRWEGYLRERINGQKPI---------FGVRRSSIIGRSSLTVEMYG 135
            L  + R K+ +L   W  Y      G+  I           ++ + +  +S +   +Y 
Sbjct: 92  SLLLMHRTKKITLVDSWGIYEANDTKGETKIPKCPTWYMRKNLKMNILSTKSDILAYVYS 151

Query: 136 NSGEE---YQIEGSFGQRLCKVLNAK-KEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFN 195
            S ++   Y I+GS+  + CK+++    + V EI+RK   +  V  G DV  L V PGF+
Sbjct: 152 GSFDKKNSYIIKGSYRCKSCKIVHVPLNKTVVEIKRKEVRTKGVRFGSDVFDLVVNPGFD 211

[Close]

BLAST of Tan0001194 vs. TAIR 10
Match: AT5G01750.2 (Protein of unknown function (DUF567) )

HSP 1 Score: 81.6 bits (200), Expect = 8.1e-16
Identity = 50/179 (27.93%), Postives = 95/179 (53.07%), Query Frame = 0


Query: 27  LTVMKTSLFFAGDGFTVYDCKGKLVFRVDSYGPDSRYKDELVLMDPHGRCLFTVRRKRPS 86
           + +++  +      F + D  G L+F+V    P     D+ VL+D  G  + T+R K  S
Sbjct: 40  MAIVRKMMSLTDGNFVITDVNGNLLFKVKE--PVFGLHDKRVLLDGSGTPVVTLREKMVS 99

Query: 87  LHQRWEGYLRERINGQKPIFGVRRSSIIG-RSSLTVEMYGNSGE---EYQIEGSFGQRLC 146
           +H RW+ +     + +  ++ V+RSS++  ++ L V +  N  E   +++++GS+ +R C
Sbjct: 100 MHDRWQVFRGGSTDQRDLLYTVKRSSMLQLKTKLDVFLGHNKDEKRCDFRVKGSWLERSC 159

Query: 147 KVLNAKKEAVAEIRRKVDASTNVLLGKDVLSLSVKPGFNGAFAMALVLVLDRINGDDGA 202
            V   + +A+     +     +V LGKD  S++V P  + AF  +LV++LD +N +D A
Sbjct: 160 VVYAGESDAIVAQMHRKHTVQSVFLGKDNFSVTVYPNVDYAFIASLVVILDDVNREDRA 216

The following BLAST results are available for this feature:

BLAST of Tan0001194 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSC7	8.9e-68	73.51	Protein LURP-one-related 5 OS=Arabidopsis thaliana OX=3702 GN=At1g80120 PE=2 SV=...	[more]
Q9LVZ8	5.8e-67	66.48	Protein LURP-one-related 12 OS=Arabidopsis thaliana OX=3702 GN=At3g15810 PE=2 SV...	[more]
Q9ZVI6	4.6e-24	43.02	Protein LURP-one-related 8 OS=Arabidopsis thaliana OX=3702 GN=At2g38640 PE=2 SV=...	[more]
A0MFL4	3.2e-17	33.51	Protein LURP-one-related 17 OS=Arabidopsis thaliana OX=3702 GN=At5g41590 PE=2 SV...	[more]
Q9LZX1	1.1e-14	27.93	Protein LURP-one-related 15 OS=Arabidopsis thaliana OX=3702 GN=At5g01750 PE=1 SV...	[more]

BLAST of Tan0001194 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998678.1	1.9e-99	86.57	protein LURP-one-related 5-like [Cucurbita maxima] >XP_022998679.1 protein LURP-...	[more]
XP_022948684.1	2.1e-98	86.51	protein LURP-one-related 5-like [Cucurbita moschata]	[more]
XP_023523506.1	2.7e-98	86.51	protein LURP-one-related 5-like [Cucurbita pepo subsp. pepo]	[more]
KAG6606800.1	1.8e-97	85.12	Protein LURP-one-related 12, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023526859.1	3.0e-97	85.78	protein LURP-one-related 5-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0001194 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KEZ1	9.1e-100	86.57	protein LURP-one-related 5-like OS=Cucurbita maxima OX=3661 GN=LOC111493264 PE=3...	[more]
A0A6J1GAM5	1.0e-98	86.51	protein LURP-one-related 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452281 PE...	[more]
A0A6J1GU66	3.6e-96	84.58	protein LURP-one-related 5-like OS=Cucurbita moschata OX=3662 GN=LOC111457578 PE...	[more]
A0A0A0KYG2	1.2e-94	85.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G005540 PE=3 SV=1	[more]
A0A5D3E0H7	2.0e-94	85.45	Protein LURP-one-related 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Tan0001194 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80120.1	6.3e-69	73.51	Protein of unknown function (DUF567)	[more]
AT3G15810.1	4.1e-68	66.48	Protein of unknown function (DUF567)	[more]
AT2G38640.1	3.3e-25	43.02	Protein of unknown function (DUF567)	[more]
AT5G41590.1	2.3e-18	33.51	Protein of unknown function (DUF567)	[more]
AT5G01750.2	8.1e-16	27.93	Protein of unknown function (DUF567)	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038595	LURP-one-related superfamily	GENE3D	2.40.160.200		coord: 20..201 e-value: 4.0E-54 score: 185.0
IPR007612	LURP-one-related	PFAM	PF04525	LOR	coord: 22..191 e-value: 1.4E-55 score: 187.8
IPR007612	LURP-one-related	PANTHER	PTHR31087	FAMILY NOT NAMED	coord: 22..202
None	No IPR available	PANTHER	PTHR31087:SF60	PROTEIN LURP-ONE-RELATED 5	coord: 22..202
IPR025659	Tubby-like, C-terminal	SUPERFAMILY	54518	Tubby C-terminal domain-like	coord: 21..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0001194.1	Tan0001194.1	mRNA