Tan0001091 (gene) Snake gourd v1

Overview


Name	Tan0001091
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	rRNA N-glycosidase
Location	LG06: 31203667 .. 31204716 (+)
RNA-Seq Expression	Tan0001091
Synteny	Tan0001091

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGATGAAATTGGTTCAGCTTTGCTTTGTTCTCGCTCTCTATCTTGGTGCTCATATTACAGAGGCTGATGTCAGCTTCTCTCTATTGGGTGCCACTGGTGCAACCTACAAACAGTTCATAAAAAATCTTCGTACTGCACTCACGGTTGGATCTCCATCAGTATATAATATACCTGTGTTGAAGAGCACAGCAATAGGATTGGCGCGCTTCCTATTAGTTCATCTTACGAATTATAATGGTGAATCTATTACTGTGGCAGTAGATATGGTGACCGTGTATGTTGTAGCATATCGAGCTGGAAACAATGCCTACTTTCTCAATGATGCTTCAGCAGAAGCCAACAATGTGCTATTTAAAGGCATCAATCACGTAAGACTTCCCTATGGAGGTAATTATGATGGCCTTGAGACAGCTGCAGGGAGAATTTCAAGAGAGAAAATTTATCTTGGATTTTCTGAAATAAGTAGTGCCATTGGCAGCATGTTCCACTACAATGCTGGTACTTCTGTTCCTAAAGCTTTTCTTGTGGTCATCCAAACAGTCTCAGAAGCTGCAAGATTTAAATATATTGAGCAAAGGGTTTCTGAGAATGTTGAGACGACGTTTCTACCAGACCCTGCCTTTTTAAGTTTAGAAAACAATTGGGGCGGTCTCTCTGAACAAATACAGATCGCACAATATCGACATGGGGAGTTTGCTCGTGCCATCCAGCTTCGATCTGTCAGCAACACTCCAATTCTTGTGACCAATGTTAATTCACCTGTTGTGAAAGGCGTTGCACTTCTACTATACTATAAAGTTAATGTTGCCACTCATAATGTTATTATAACGTCGTTGTCAACCTATTAGTACTTATCAAACTACTATGTGCTTGTATGTGCAAGTATGAGAATAAAGGTTTAAGCCTAAACTTCTAAGCTTTCTTTTTTATTCGTTGATCTTTAAAGTTTTTATAATATAGTTATTAAAGATAACAAACCTCCACATATCTCCACAATGGTATGATATTGTCCACTTTGGGCATCAACCCTCATGGTTTTGTTTTTTG

mRNA sequence

AAAAGATGAAATTGGTTCAGCTTTGCTTTGTTCTCGCTCTCTATCTTGGTGCTCATATTACAGAGGCTGATGTCAGCTTCTCTCTATTGGGTGCCACTGGTGCAACCTACAAACAGTTCATAAAAAATCTTCGTACTGCACTCACGGTTGGATCTCCATCAGTATATAATATACCTGTGTTGAAGAGCACAGCAATAGGATTGGCGCGCTTCCTATTAGTTCATCTTACGAATTATAATGGTGAATCTATTACTGTGGCAGTAGATATGGTGACCGTGTATGTTGTAGCATATCGAGCTGGAAACAATGCCTACTTTCTCAATGATGCTTCAGCAGAAGCCAACAATGTGCTATTTAAAGGCATCAATCACGTAAGACTTCCCTATGGAGGTAATTATGATGGCCTTGAGACAGCTGCAGGGAGAATTTCAAGAGAGAAAATTTATCTTGGATTTTCTGAAATAAGTAGTGCCATTGGCAGCATGTTCCACTACAATGCTGGTACTTCTGTTCCTAAAGCTTTTCTTGTGGTCATCCAAACAGTCTCAGAAGCTGCAAGATTTAAATATATTGAGCAAAGGGTTTCTGAGAATGTTGAGACGACGTTTCTACCAGACCCTGCCTTTTTAAGTTTAGAAAACAATTGGGGCGGTCTCTCTGAACAAATACAGATCGCACAATATCGACATGGGGAGTTTGCTCGTGCCATCCAGCTTCGATCTGTCAGCAACACTCCAATTCTTGTGACCAATGTTAATTCACCTGTTGTGAAAGGCGTTGCACTTCTACTATACTATAAAGTTAATGTTGCCACTCATAATGTTATTATAACGTCGTTGTCAACCTATTAGTACTTATCAAACTACTATGTGCTTGTATGTGCAAGTATGAGAATAAAGGTTTAAGCCTAAACTTCTAAGCTTTCTTTTTTATTCGTTGATCTTTAAAGTTTTTATAATATAGTTATTAAAGATAACAAACCTCCACATATCTCCACAATGGTATGATATTGTCCACTTTGGGCATCAACCCTCATGGTTTTGTTTTTTG

Coding sequence (CDS)

ATGAAATTGGTTCAGCTTTGCTTTGTTCTCGCTCTCTATCTTGGTGCTCATATTACAGAGGCTGATGTCAGCTTCTCTCTATTGGGTGCCACTGGTGCAACCTACAAACAGTTCATAAAAAATCTTCGTACTGCACTCACGGTTGGATCTCCATCAGTATATAATATACCTGTGTTGAAGAGCACAGCAATAGGATTGGCGCGCTTCCTATTAGTTCATCTTACGAATTATAATGGTGAATCTATTACTGTGGCAGTAGATATGGTGACCGTGTATGTTGTAGCATATCGAGCTGGAAACAATGCCTACTTTCTCAATGATGCTTCAGCAGAAGCCAACAATGTGCTATTTAAAGGCATCAATCACGTAAGACTTCCCTATGGAGGTAATTATGATGGCCTTGAGACAGCTGCAGGGAGAATTTCAAGAGAGAAAATTTATCTTGGATTTTCTGAAATAAGTAGTGCCATTGGCAGCATGTTCCACTACAATGCTGGTACTTCTGTTCCTAAAGCTTTTCTTGTGGTCATCCAAACAGTCTCAGAAGCTGCAAGATTTAAATATATTGAGCAAAGGGTTTCTGAGAATGTTGAGACGACGTTTCTACCAGACCCTGCCTTTTTAAGTTTAGAAAACAATTGGGGCGGTCTCTCTGAACAAATACAGATCGCACAATATCGACATGGGGAGTTTGCTCGTGCCATCCAGCTTCGATCTGTCAGCAACACTCCAATTCTTGTGACCAATGTTAATTCACCTGTTGTGAAAGGCGTTGCACTTCTACTATACTATAAAGTTAATGTTGCCACTCATAATGTTATTATAACGTCGTTGTCAACCTATTAG

Protein sequence

MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKSTAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTVSEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSVSNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY

Homology

[Close]

BLAST of Tan0001091 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.1e-116
Identity = 217/282 (76.95%), Postives = 244/282 (86.52%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITE-ADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVL 60
           M+ +    VLALY+GAH+TE  D++FSL+GATGATYK FI+NLRT LTVG+P VY+IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KSTAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKG 120
           ++ A GLARF LV LTNYNGES+TVA+D+V VYVVAYRAGN AYFL DAS EANNVLF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 INHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQT 180
           INHVRLPYGGNYDGLETAAGRISRE I LGFSEISSAIG+MF +N GTSVP+AF+V+IQT
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRS 240
           VSEAARFKYIEQRVSENV T F PDPAFLSL+N WG LSEQIQIAQ R GEFAR ++LR+
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VSNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY 282
           VSNTP  VTNVNSPVVKG+ALLLY++VNV T NV   SLSTY
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNVGTDNVFAMSLSTY 282

[Close]

BLAST of Tan0001091 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 3.6e-56
Identity = 126/269 (46.84%), Postives = 177/269 (65.80%), Query Frame = 0


Query: 3   LVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKST 62
           LV    +L L+L     E DVSF L GAT ++Y  FI NLR AL      +Y+IP+L+S+
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSS 64

Query: 63  AIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASA-EANNVLFK-GI 122
             G  R+ L+HLTNY  E+I+VA+D+  VY++ YRAG+ +YF N+ASA EA   +FK  +
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAM 124

Query: 123 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 182
             V LPY GNY+ L+TAAG+I RE I LG   + SAI ++F+YNA  S   A +V+IQ+ 
Sbjct: 125 RKVTLPYSGNYERLQTAAGKI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQST 184

Query: 183 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 242
           SEAAR+K+IEQ++ + V+ TFLP  A +SLEN+W  LS+QIQIA   +G+F   + L + 
Sbjct: 185 SEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINA 244

Query: 243 SNTPILVTNVNSPVVKGVALLLYYKVNVA 270
            N  + +TNV++ VV     LL  + N+A
Sbjct: 245 QNQRVTITNVDAGVVTSNIALLLNRNNMA 270

[Close]

BLAST of Tan0001091 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 4.6e-56
Identity = 128/269 (47.58%), Postives = 176/269 (65.43%), Query Frame = 0


Query: 3   LVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKST 62
           LV    +L L+L A   E DVSF L GAT ++Y  FI NLR AL      +Y+IP+L+ST
Sbjct: 5   LVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPY-ERKLYDIPLLRST 64

Query: 63  AIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASA-EANNVLFKGIN 122
             G  R+ L+HLTNY  E+I+VA+D+  VYV+ YRAG+ +YF N+ASA EA   +FK   
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAK 124

Query: 123 H-VRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 182
             V LPY GNY+ L+ AAG+I RE I LG   + SAI ++F+YNA  S   A +V+IQ+ 
Sbjct: 125 RKVTLPYSGNYERLQIAAGKI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQST 184

Query: 183 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 242
           SEAAR+K+IEQ++ + V+ TFLP  A +SLEN+W  LS+QIQIA   +G+F   + L + 
Sbjct: 185 SEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINA 244

Query: 243 SNTPILVTNVNSPVVKGVALLLYYKVNVA 270
            N  + +TNV++ VV     LL  + N+A
Sbjct: 245 QNQRVTITNVDAGVVTSNIALLLNRNNMA 270

[Close]

BLAST of Tan0001091 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 6.1e-56
Identity = 123/263 (46.77%), Postives = 178/263 (67.68%), Query Frame = 0


Query: 9   VLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKSTAIGLAR 68
           +LA+++ A   EADV FSL G++  +Y +FI +LR AL   + +VYNI +L S+A G +R
Sbjct: 8   ILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALP-SNGTVYNITLLLSSASGASR 67

Query: 69  FLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAE-ANNVLFKGINHVRLPY 128
           + L+ L+NY+G++ITVAVD+  VY++ Y   + +YF N++ A+ A+  +FKG   V LPY
Sbjct: 68  YTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPY 127

Query: 129 GGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTVSEAARFK 188
            GNY+ L+TAAG+I REKI LGF  + SAI ++FHY++ T+   AFLV+IQT +EA+RFK
Sbjct: 128 SGNYEKLQTAAGKI-REKIPLGFPALDSAITTLFHYDS-TAAAAAFLVIIQTTAEASRFK 187

Query: 189 YIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSVSNTPILV 248
           YIE ++ E +    +P  A +SLEN W  LS+QIQ+AQ  +G F   + +       + +
Sbjct: 188 YIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEI 247

Query: 249 TNVNSPVV-KGVALLLYYKVNVA 270
           TNV S VV K + LLL YK NVA
Sbjct: 248 TNVTSKVVTKNIQLLLNYKQNVA 267

[Close]

BLAST of Tan0001091 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 2.3e-55
Identity = 129/278 (46.40%), Postives = 181/278 (65.11%), Query Frame = 0


Query: 1   MKLVQLCF-VLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVL 60
           +K + L F ++A+++G    + DV+F L  AT  TY +FI++ R  L   S  VY+IP+L
Sbjct: 2   VKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLL 61

Query: 61  KSTAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKG 120
            ST     RF+L++LT+Y  E+I+VA+D+  VYVVAYR  + +YF  ++  EA N+LFKG
Sbjct: 62  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 121

Query: 121 INHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQT 180
              + LPY GNY+ L+TAA +I RE I LG   +SSAI ++F+YNA  S P A LV+IQT
Sbjct: 122 TRKITLPYTGNYENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQT 181

Query: 181 VSEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRS 240
            +EAARFKYIE+ V++ V T F P+ A +SLEN W  LS+QI +AQ + G+F   + L  
Sbjct: 182 TAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIK 241

Query: 241 VSNTPILVTNVNSPVVKG-VALLLYYKVNVATHNVIIT 277
            +     VTNV+S VVKG + LLL  + + A  N I T
Sbjct: 242 PTGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITT 276

[Close]

BLAST of Tan0001091 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 476.9 bits (1226), Expect = 1.2e-130
Identity = 248/281 (88.26%), Postives = 261/281 (92.88%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLK 60
           MKLV    VLALY+GA +TEADV+FSLLG+T ATYKQFI+NLR ALTVGSP+V NIPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  STAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120
            TA GLARFLLVHLTNYNGESITVAVD+V VYVVAYRAGNNAYFLNDASAEANNVLFKGI
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 180
           NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAF+V+IQTV
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 240
           SEAARFKYI+QRVSENV+T FLPDPAFLSLEN WGGLSEQIQIAQYR GEFAR I+LR+V
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 SNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY 282
           SNTP  VTNVNSPVVKGVALLLYYKVNVAT NVI  SLSTY
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVATDNVIRMSLSTY 281

[Close]

BLAST of Tan0001091 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 419.5 bits (1077), Expect = 2.3e-113
Identity = 217/282 (76.95%), Postives = 244/282 (86.52%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITE-ADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVL 60
           M+ +    VLALY+GAH+TE  D++FSL+GATGATYK FI+NLRT LTVG+P VY+IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KSTAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKG 120
           ++ A GLARF LV LTNYNGES+TVA+D+V VYVVAYRAGN AYFL DAS EANNVLF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 INHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQT 180
           INHVRLPYGGNYDGLETAAGRISRE I LGFSEISSAIG+MF +N GTSVP+AF+V+IQT
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRS 240
           VSEAARFKYIEQRVSENV T F PDPAFLSL+N WG LSEQIQIAQ R GEFAR ++LR+
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VSNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY 282
           VSNTP  VTNVNSPVVKG+ALLLY++VNV T NV   SLSTY
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNVGTDNVFAMSLSTY 282

[Close]

BLAST of Tan0001091 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 411.8 bits (1057), Expect = 4.9e-111
Identity = 211/281 (75.09%), Postives = 241/281 (85.77%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLK 60
           M+ +   FVLAL  GA   EADVSFS+LGAT  TY+QFI+NLR+ LT+ SP VY IPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  STAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120
           STA+G ARF+LVHLTNYN ESITVA+D+V +YVVAYRAGNNAYFLNDASAEANNVLFKGI
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 180
            HVRLPY GNYDGLETAAG+ISREKI LGFSEISS+IG+MFH+N GTS+ +AF+V+IQ+V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 240
            EAARFKYIEQRVSENV+T F PDPAFLSLEN W  LSEQIQIAQ R GEFARAI+LR+V
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 SNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY 282
           SN PI+VTNV+SP+VKG+ALLLYYKV VA  N+I     TY
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVAIDNIIRMPTMTY 281

[Close]

BLAST of Tan0001091 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 406.8 bits (1044), Expect = 1.6e-109
Identity = 214/282 (75.89%), Postives = 247/282 (87.59%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLK 60
           M+L+   F LALYLGA   EADVSFS+LG+T  TYKQFIKN+R+ALTVGS  VY+IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  STAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120
           +TA G ARFLLVHLTNYN ESI VA+D+V VYVVAYRAGNNAYFLNDASAEA+ VLF+GI
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 180
           NHV+L Y GNYDGLETAA +ISREKI LGFSEISS+IG+MFH+NAGTSVP+AF+V+IQ+V
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 240
           SEAARFKYIEQRVSENV+TTF PDPAFLSL+N W  LSEQIQIAQ R GEFARA++LR+V
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 SNTPILVTNVNSPVVKGVALLLYYKVNVA-THNVIITSLSTY 282
           SN PILVT+V+SPVVKG+ALLLYYKV+V    N+I   ++TY
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSVVMDDNIIKVPMATY 281

[Close]

BLAST of Tan0001091 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 354.4 bits (908), Expect = 9.2e-94
Identity = 179/245 (73.06%), Postives = 209/245 (85.31%), Query Frame = 0


Query: 21  ADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKSTAIGLARFLLVHLTNYNGE 80
           ++VSFSLLGATG TY+QFI+NLR ALT+ S  VYNIPVL +TA+  ARF+LVHLTNY  E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGNYDGLETAAGR 140
           +ITVA+D+V VY+VAY+AGN AYFL DAS EA +VLFKGI    LPY GNYDGLETAAG+
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTVSEAARFKYIEQRVSENVETT 200
           ISREKI LGFSE+ SAIG+M+HYNAGTSVP+AF+V+IQT+SEAARFKYIE +VS+NVET 
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSVSNTPILVTNVNSPVVKGVAL 260
           F PDP FLSLEN W  LSEQ+QIAQ R GEFAR I++RSV+N PILVTNV S VV+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

Query: 261 LLYYK 266
           LLY K
Sbjct: 275 LLYSK 279

[Close]

BLAST of Tan0001091 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 6.0e-131
Identity = 248/281 (88.26%), Postives = 261/281 (92.88%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLK 60
           MKLV    VLALY+GA +TEADV+FSLLG+T ATYKQFI+NLR ALTVGSP+V NIPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  STAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120
            TA GLARFLLVHLTNYNGESITVAVD+V VYVVAYRAGNNAYFLNDASAEANNVLFKGI
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 180
           NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAF+V+IQTV
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 240
           SEAARFKYI+QRVSENV+T FLPDPAFLSLEN WGGLSEQIQIAQYR GEFAR I+LR+V
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 SNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY 282
           SNTP  VTNVNSPVVKGVALLLYYKVNVAT NVI  SLSTY
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVATDNVIRMSLSTY 281

[Close]

BLAST of Tan0001091 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.4e-111
Identity = 211/281 (75.09%), Postives = 241/281 (85.77%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLK 60
           M+ +   FVLAL  GA   EADVSFS+LGAT  TY+QFI+NLR+ LT+ SP VY IPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  STAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120
           STA+G ARF+LVHLTNYN ESITVA+D+V +YVVAYRAGNNAYFLNDASAEANNVLFKGI
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 180
            HVRLPY GNYDGLETAAG+ISREKI LGFSEISS+IG+MFH+N GTS+ +AF+V+IQ+V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 240
            EAARFKYIEQRVSENV+T F PDPAFLSLEN W  LSEQIQIAQ R GEFARAI+LR+V
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 SNTPILVTNVNSPVVKGVALLLYYKVNVATHNVIITSLSTY 282
           SN PI+VTNV+SP+VKG+ALLLYYKV VA  N+I     TY
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVAIDNIIRMPTMTY 281

[Close]

BLAST of Tan0001091 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 7.6e-110
Identity = 214/282 (75.89%), Postives = 247/282 (87.59%), Query Frame = 0


Query: 1   MKLVQLCFVLALYLGAHITEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLK 60
           M+L+   F LALYLGA   EADVSFS+LG+T  TYKQFIKN+R+ALTVGS  VY+IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  STAIGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120
           +TA G ARFLLVHLTNYN ESI VA+D+V VYVVAYRAGNNAYFLNDASAEA+ VLF+GI
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTV 180
           NHV+L Y GNYDGLETAA +ISREKI LGFSEISS+IG+MFH+NAGTSVP+AF+V+IQ+V
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSV 240
           SEAARFKYIEQRVSENV+TTF PDPAFLSL+N W  LSEQIQIAQ R GEFARA++LR+V
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 SNTPILVTNVNSPVVKGVALLLYYKVNVA-THNVIITSLSTY 282
           SN PILVT+V+SPVVKG+ALLLYYKV+V    N+I   ++TY
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSVVMDDNIIKVPMATY 281

[Close]

BLAST of Tan0001091 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 4.5e-94
Identity = 179/245 (73.06%), Postives = 209/245 (85.31%), Query Frame = 0


Query: 21  ADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKSTAIGLARFLLVHLTNYNGE 80
           ++VSFSLLGATG TY+QFI+NLR ALT+ S  VYNIPVL +TA+  ARF+LVHLTNY  E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGNYDGLETAAGR 140
           +ITVA+D+V VY+VAY+AGN AYFL DAS EA +VLFKGI    LPY GNYDGLETAAG+
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTVSEAARFKYIEQRVSENVETT 200
           ISREKI LGFSE+ SAIG+M+HYNAGTSVP+AF+V+IQT+SEAARFKYIE +VS+NVET 
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSVSNTPILVTNVNSPVVKGVAL 260
           F PDP FLSLEN W  LSEQ+QIAQ R GEFAR I++RSV+N PILVTNV S VV+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

Query: 261 LLYYK 266
           LLY K
Sbjct: 275 LLYSK 279

[Close]

BLAST of Tan0001091 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.5e-84
Identity = 171/265 (64.53%), Postives = 203/265 (76.60%), Query Frame = 0


Query: 12  LYLGAHI--------TEADVSFSLLGATGATYKQFIKNLRTALTVGSPSVYNIPVLKSTA 71
           L  GAH+          A++SFSLLGAT  TYKQFI +LR ALTV S  VY+IPVL +TA
Sbjct: 17  LNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATA 76

Query: 72  IGLARFLLVHLTNYNGESITVAVDMVTVYVVAYRAGNNAYFLNDASAEANNVLFKGINHV 131
            GLARF+LV+LTNY GESITVA+D V VY+VAY+AGN AYFL D S EA++VLFKG  H 
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 132 RLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFLVVIQTVSEA 191
            L Y GNYD LE  AG+ISRE I LGFSE+SS+IG+MFHYNAG SVPK+F+V+IQ +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEA 196

Query: 192 ARFKYIEQRVSENVETTFLPDPAFLSLENNWGGLSEQIQIAQYRHGEFARAIQLRSVSNT 251
           +RFKYIE R  ENVE+ F PDP +LSLEN W  LSEQ+Q AQ   G+F   +Q+RSVSN 
Sbjct: 197 SRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

Query: 252 PILVTNVNSPVVKGVALLLYYKVNV 269
           P+LV++VNSPVVKG+ALLL  K  V
Sbjct: 257 PVLVSDVNSPVVKGMALLLCNKPGV 281

The following BLAST results are available for this feature:

BLAST of Tan0001091 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	3.1e-116	76.95	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P09989	3.6e-56	46.84	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24478	4.6e-56	47.58	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
Q00465	6.1e-56	46.77	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P24817	2.3e-55	46.40	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Tan0001091 vs. NCBI nr


Match Name	E-value	Identity	Description
BAG12408.1	1.2e-130	88.26	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
P98184.1	2.3e-113	76.95	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
XP_022158192.1	4.9e-111	75.09	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158193.1	1.6e-109	75.89	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158191.1	9.2e-94	73.06	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of Tan0001091 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B1B626	6.0e-131	88.26	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
A0A6J1DWK4	2.4e-111	75.09	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	7.6e-110	75.89	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
A0A6J1E093	4.5e-94	73.06	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1IUB2	2.5e-84	64.53	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]

BLAST of Tan0001091 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 143..157 score: 42.98 coord: 33..46 score: 42.29 coord: 173..193 score: 64.79 coord: 81..96 score: 46.05 coord: 205..222 score: 48.68
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..222 e-value: 7.5E-32 score: 110.6
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 22..275
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..269 e-value: 3.5E-27 score: 96.1
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 22..185 e-value: 1.6E-42 score: 147.1
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 22..275
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 177..193
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 22..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0001091.1	Tan0001091.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity