Tan0001044 (gene) Snake gourd v1

Overview
NameTan0001044
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionUBC core domain-containing protein
LocationLG05: 10004748 .. 10012728 (+)
RNA-Seq ExpressionTan0001044
SyntenyTan0001044
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCGAAGCATTTACAAACCGACCCCCACGGTGCTCTCTTTCCAGCTCCCCCTCCCTCTAACTCTCTCTCTCTCTCTCTCTCAATTCTTGGTTCTCTGGCAATATATTCTCTGGTTCGAAGACGGTGTTCGAAGGAACCCTCCGTTCAAAATCAGACACCTCCTTTTTCTTCATCGCATCGCCTTCTTTCCCTCCAATCGACGACGATTCTCAGATTGCCTTTCTCTTTGCAGTTCCTTTTTCTCTGCCTCGGAAGCTCAGGTTCTCATTTCCTTAGCTCGTCTGTTTTCGTTTCTGTATTGGATTGAGCTTTTCGATGGAATAGTTGTTCTTTATTCTTTGGATAACTGGTGGGGTGTTTGAATTTAGCTCGAGGGATTGATCTGAGAAGGTCTCTAATCTTGGCTTTTAGCTTCTTTTGTTTCTTTCTTTGTTTTTCATTTCGATGAATGGTTTTTCTCGTAATTATGGCCTGCTTTGGGCATTTGAATGCGAGAACGGGAGAGGCGTAAATGGTTTTAGCCATTATTCCTTCCTCTCGTGGCGTATGATTCAATCAACTTGATGATTTATCCTAAGACGTCCATTGGGTGCCTTCTTATGTGCAGTCTGATGATTTCCCTTTAATGTTGGCTTCATAGAATCAATGGATGATTATACCAATCGCTGGTATAAAAGGGCTCCTTCTCACGAAGTTTTTTTTTTCTCCCCTTCTTTTGGTTCTGGTCTTACTAAACATATTAGCAAATGAACCCACAATTTTTCATTTCTGTTTGTGTTGGTCACTCGTCTTTCCGTTTATTTCCACCCCTTGTTTCTTTGTTGTTGTTTTTTTTTTTTTTGAATTCAAGAACTGAAAATCTAACGTGGTGATGATTGAGATATACTAATGGAAATATCTTTGGACAGTGCTCTAAGTAGCAACAGAAAGCTACTTGCAAAATATCTCAACCTCCTGATTCTTTATTCCGAAAATATGCTTTGAATGTCCACCTCATGCTGCTTTGTTCATCCTGTTAATTTTCCCTACTTGATGAAAATATGAAGGAAAAAGAAAGAGCAGTGAGCTTATTTGACTTGACTGCTGCTTCCAGTAACATTACAACTAGTTTGTGTATTATCTTTGTGCTTACTTACAGACTAAGGGCCCATGGATTTTGTTTGTTCACTTTCTTGGTTGCCAGGATTAACACTTAGGGAGCATGAATGCTCTTATTACATGTGGAGTTAGGTATCCAACATGAACATCTTTTGGCATATGATTTGAAGTTCTCAATTTTTTTTTTTGCTTTGGGGACACTGGACACATTAGTGCACTCAAGAGACACATGTGAGGTGGGTCTGGCAGAAAAAGTAAAAAGAAAAAAAATTAATTACTGATAATAATAATAATAATAATAATAATAATAATCAAAATATAGTTCTCCGTTAAAACCAGGAATATTTACACCAAGTAACTAAGACAAACGAGCAAAAGCATAAAACCTACAGAAAACAGAAAAAAGGAGAATAAATACATTTTACTTTGTAAGTCTTTAATGCAGTACCTGAGAAATGTGAAAATGATGCTTGAGGTGATCTTTTTTTTGGAGAATATTATATTTTGTGCAATGCTTGACTACATATTGTTGTTCGAGAATACCATGTCAATGAAGTAATATATTTGACTTCATGACATTTAAATTTGTCTTTTTTTTGTTTGTTTTTGATATCCATGAGTATTCGGAGGCTAGCTTGCACCCTAAATTTGTTTAAAATTGAATGGATGAGAATACAATTCTGTACCTTCAATTCTTTAGAAGAACGCACAAAAGTATCTAAAGTATTTCTATTCAATGTGCCCCAACCATGTGCATGGCCTCATTCTTTTAAAAACTGATATGTTGACTTTCCGCATTGCTTCATATATGTGTCCCATTTCTGACTTTGTGCTTCTTAGATTTGAACTTTCACAAATGTATTTTACAGAATTGAAATGAAAATTATCTCAATGTACTGAGTTACATATGTGAGTGATAGCTTTACTCGTCCTTCTCCCTTCTTTCTTTTCAGATGGAGCAACGGGATTCTGTGGACAAGGAGTCTCTTATTGGTACTTGCAATAGTTATATGAATGTGGATGGTGTTTCTCCAAGTGGAAATATTTCCACGCCAACTATAAATAATGATGAAGCTAGAAGAACCAATGAGAATACAAGTGACAAACCTAATATTCCACATATATACAGACAAGACATTGTAAAAAGCAAAGGCAGTGGTATGATAGGGATTGTGACTGAAGTTGCTGGTGATGCTGATTCAGATAGTGACATTACTGATGATGAAGATGACGACGATGACGGCGAGGATGGTGTTGATGACGATGGTTGTGATGATTTTGATGGCGATGGTGAGAAAGAAGGTCAAAACAAGGAAAATAATGGCGATGACAGTAATGCTAAAAATTCCAACAAAGATAACTATAAGAGCCAACCCCTTCCTGTTGATGAAGTTCGAGTCCTTTGGATGGATGAAAGTGAGACAACTCAGAATGTTAATGATTTAACAGTTATAGATAGGGGATTTTTACACGGTGATTTTGTTGCTGCTGTTTCCGATCCAACAGGACAAGCAGGTGTTGTGGTAGATGTAAATATCTCTGTTGATTTGTTGGTTCCCGATGGATCAATTATGAAAGATATATCATCTAAAGACTTGAAACGTGTCAGAGATTTCACTGTGGGGGATTATGTCGTCCTCGGTCCATGGTTGGGTAGAATTGATGATGTCTTGGATAATGTGACTGTGATGTTTGATGATGGCTCCAAGTGTAAAGTCACAAAGGCAGAGCCATTGCGGCTCAAACCAGTTTCTAAGAATATCCTTGAAGATGCAAACTTTCCTTACTATCCTGGGCAGCGTGTAAGGGCGAGTTCTTCAACAGTTTTTAAGAATTCTAAATGGCTTTCTGGTTTATGGAAACCTAATCGCATGGAAGGTACTGTCACTAAAGTGACAGTTGGATCAGTGTTTATTTATTGGATAGCATCTGCTGGATATGGACCAGATTCTTCTACCACCCCAGCTGAAGAACAGACTCCAAAGAACTTGAGACTATTAACTTGTTTCTCACATGCGAATTGGCAATTGGGTGACTGGTGTCTTCTTCCCTCATCATTCTCCTCTGGCTCAACTAAGGACTCATCGCAAACGGAGCTTCCTGATTCAGTGACCAATACATTAGATTCTGCACAAACGGTGGGTGCATGTGATTCAGAGGATACTGTGCTAGATGAATTAAGTGGGACAACTGAATCCACTGATCTTGATTCCATTAGTGCATGTGATGGAAATTATAGGAATCCAGAATGTAATATTTTGCCCGAATCCAGTAGTTCTAGAGCTCCAAAGGAGAATACCCATGAGACTTGGCCTCTACATCGGAAAAAAATTCGTAAAGTTGTCGTCAGGAGAGATAAGAAGGCTAGAAAGAAAGAGGAAAACTTTGAGAGGGCCCTTTTAATTATCAATACTAAGACAAAGGTTGATGTTGCATGGCAGGATGGCCAGACAGAACTTGAACTGGATTCAACAAGCTTGATTCCAATTGATAATCCAGGTGATCATGAATTTGTTCCTGAACAGTATGTGGTTGAGAAGGCCTCTGATAATGATGATGATGTTACCGAAAGCAGGCGAGTTGGAGTTGTGAAAAGTGTTCATGCAAAGGAGCGAACAGCCTGTGTAAGGTGGTTAAAACCAGTATCCAGGGCAGAGGACCCTCGAGAATTTGACATAGAGGAAATTGTTAGTGTGTATGAGCTAGAGGGACATCCGGATTATGACTACTGTTATGGTGACGTGGTTGTTCGATTGTCACCTATTTCTGATTCTACAGAAGCAATGTCTTCTGAAAACAATACTGAGGAATTAAATGGGGAAAGTTCAACAAATGGGGTGATGAGTTGTACAGAAAAGGGTTCAGGAAGCCCGAAAATAGAAGATCCATCATGTAGTGATGATTGCATCGACTTCTCAGATCTCTCTTGGGTTGGAAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACTTGGGCTAATGGCATGGTTTCAACGGTATTTTTCATTTCTTAATTTCTTTGAAAATGATACTTAGTCAGACCAACTTGCTTATGATCCCCTTCTAGAAGGAAGAATGCCTTATAATTGGAATTAAACAAATTTGTTATAAAGGGCAGGATTGGGAGCTGTTATTCCTGTATTTTCATAGGAATTAGATATTATTTTTAAGTGTCATATCTGCTTCTTGGCTTTTAATTAGTTGATCTGGAAATATTAAAAATGTTATTTTTGTCTGTTTGCTTTGACTTGATGGAGGCTATAATCTGTGAGAGTACTAGTATGGGATAGTAGAGGGTCTTTGTGAGAGTACTAATACACCCTAGTTAAAAACTCCAAAATACCCCTTACTAATATACTACTAATCTCCCACAAAGACCCTCTACTATCCCATACTAGTACTCTCACATAATCTAACACTTCTATTTGGTGATTTAGTTTTTAATGGTTTTCACTTCTCTCACCTCCAGAGTTGAGAACTTAGATGCCAAAATGTTTGTTATTTTTCTAAGGTTGGCCCTCAAGCAATTTATGTTGTTGGTCGAGATGATGACGATGAATCAATTGCTGCTGGAAGTGAAGTAAGTAATGGTGCAGCAAGTTGGGAAACAGTTGATGATGATGAAATAGATTCTGTTGAGAATGCTAAGGAGGTAATATTCTTTCGATACACTAAAGTTAGCATATCTTGTATTTACTTAATTTTGAATGGTTCTAATAGTATACTGTCTAGATTTTCTGTCAGAGAAACTGAAGAATAAACAACGAGTAACATGTAATGATGAGGGCTGCTATGGACTTTTGTTTTAATTATGCGTATTAGTTGCAAAAGGTCATAGTCAAGAACTCCTGTGTGTCTCTTTAATTGCAATTTTCTTTAGTGATGGAATTCAAAATGTATTTTGATGGATAGTATATTCATCTCTCATTCTTTCACTCACTCTCCAAACTATCATTTCTTTTTCAGTTTCTGCATCCATGGTTTTGGAAAAATTTTTCTTATCATTTCTTATTAATGTCCCTCCATATGTTATGCCCCTTGTGCTAATTTTTGACAGTTCGACAGTTCTTATAAATTGAAATCTGATGTTTATTGTGCCCTTAAATGTTCATTTGCTTACCTTTGCTTGTAACGTTGTTATCTACAGTTTTAAGAATAATTACACCTGAGGCATCTCTTTCTAAGTAAAATAATGACCCACGTCAATGTCTCTAGGATATTGAGCTGCAGGATACTGGTGTCAATTCTGAAGAGGAAGATAGTGAACAGAATAATTCAGGAAGAAATCTAGCTCTTTCTGTTCCACTTGCTGCACTTCGATTTGTTACTAGATTGGCTGCTGGAATATTCTCACGAGGACCAAGAAATCCAGATTCAATGGATTTGGATTCCCACAGTGAAAGTGAGACTCAATCCTTGGAAATTCAAGCTTCAGAGGAAAAAGATTCTGGGCTTCAGTCTAGCTCTCTTAAATCCAATTCTTTTGATGCTTCTGATATGAACTCTGATTGTGGAAGAGGAGAGGATGATGATGCTTCAGAGTCATCAAAAGTGTTGGAATCAGCAGAAGCATCAAGCAACCTGAGGACTGCAGAATCCGATGGTTCAGCATGCCATGAGGATGGCACCTGCAGCTTCAAAGGCTTTGATATAGCCAAAGATCCTTTGGACCATTATTTTCTTGGTACAAATGGACAAGTAAGTAATTTATAAAATTCTCCAAACCTTGATATATTTGTGAAGCTTCGATACCACTTTGATGTTGTAAGAATAATTCTGTGTGCGATATTTGTTTTAGGGTCTTCTTTTTCCCTGTTCATGAATACTTTGATGTTTCTGGTTGTTCAGATTGGTTTACACGCGTCCTTACTTGAAAGATTCCTCACAACTTTGAATGATTGAACTTTGTTGCATGCAGACTAATAATGGAAGGAAATGGCTCAAGAAAATCCAGCAAGATTGGAGTATCCTCCAGAACAATCTGCCAGGTAAAAACAATGCCTATCTTTGCTTCATGTTGTCACGTCTCCTTAGATTGACATCCATGTATTTGTTAGCTGAGCCATGAATGAATTCTTGTAACAGATGGGATATACGTTCGAGTCTACGAGGATCGAATGGACCTTTTAAGAGCAGTCATTGTTGGGGCCTATGGGACTCCTTACCAAGATGGCCTCTTCTTCTTTGATTTTCATCTCCCACCAGAGTATCCTGACGTTCCACCAGTAAGTAATACTCGCCTTTTAATCATTCCACCTGTCATAGCATTAGGTCACTTCTACTATCTGGGTTATCTTATATATAACTCACTGATAGAGTTTGTTATGATGCAGTCAGCATATTATCATTCAGGTGGGTGGCGGATAAATCCCAATCTTTATGAGGAAGGCAAAGTCTGCCTTAGCCTTTTGAATACTTGGACTGGCAGAGGAAATGAAGTATGGGATCCAAAGTCCTCTAGCATCCTTCAAGTGTTGGTCTCGCTTCAGGGATTAGTGTTGAATTCTAAACCATATTTTAATGAAGCTGGATATGACAAGCAAATTGGGACAGCCGAAGGAGAGAAAAATTCTTTGTCGTACAATGAAAACACTTTCTTATTAAGCTGCAAGACAATAATGTATCTTATGAGGAAGCCTCCCAAGGTGGGTAATCTTTTTTATCATGATTATTCATTTTCATTTTATATCTTTGTTCTGAACTTTTTCTGAACCACACAATCTTCAAACGTCGTGGGCCAATTGTTTATTTTTTCTTAATTAAACTACGTAGTTTATTTATATGTTCAGATGATAGTTCTCTCTCACACATTTTAGAGCATCCTGTTATGTAGTTCAACGTTAAGTCGGTCACTGTTCTCCATCCTCGATAGTATTCTCTTTTCTTTGACTGGATTAAGAGCACTTGGGCATTTTGGAATCCAGTTAGCTGTACTACAGCGTTTTCAGCAAATGTGTTGTTTATTTGACATTTGTAATTTCATGCCATAGAAAATCTGATTAGATTTTTCCCATTAACCCTCTAAATGGGTTCAGATATTGCATTGCCTTAACATGCTTGCTCGATTGTGTTTGGCTCATAGTTTTGTCTAAACTTGGGTTTTTTAAGTATGTGTTTGTGATAAAAAGTTTAGACAATTTATTTGTGCTATAATCAGAAAGCTCATGATGTAATTACTGCCAGTGATATATTGACTTATTGAAGTTTGTTCATGAATAGGACTTTGAAGAACTCGTTAAAGAACATTTCAGGAGGCGTGGATATTACATCCTGAAGGCCTGTGATTCATACATGAAAGGTCACTTAATTGGCTCCCTTACAGAAGATGCCTCCATAAGAGAAGAAAGCGATCCTAACTCCACGTCAGTCGGATTTAAGCTTATGCTCGCTAAGATTGTTCCGAAGCTCTTCTCGTCGTTGAACGAAGTCGGAGCTGATTGTCACGATTTTAAGCATTTTCAACAGTTGTAGCATAAAAAAAACTCCAAATGACAAGTTATCATTTACATACAGCTGACAAGGTAATTTTTTCTTTTTTCTATTCATTCCCATTCATGTTAAAGTCTTCTTTTCTTTACCCATTATGATTAATGATGCACCTTGTTTATAAGTTTGAACTGGGCCCTCATGCCTTTGAAAGCTTCATAGAATTCTAGGAGCTGGGTTTTTGGGACTACTCTCTGGTCTCTGTGATCCATTTTTTTTCCTCTAAAAAAAAAAGAACGAAAGAAAGATTACATTGCAAGTTAGGAATTAGTTGATCTTGTTCTGTCTTAGGTTGGTTTGTGCCTAAAATGGACTTTATTGAGATTTTTTTTGTTTTACTGCGGTTAAAGAATTGCTCAAAGAAGAGTAAACAAGTATGATATTTTGTGCTGC

mRNA sequence

ATTCGAAGCATTTACAAACCGACCCCCACGGTGCTCTCTTTCCAGCTCCCCCTCCCTCTAACTCTCTCTCTCTCTCTCTCTCAATTCTTGGTTCTCTGGCAATATATTCTCTGGTTCGAAGACGGTGTTCGAAGGAACCCTCCGTTCAAAATCAGACACCTCCTTTTTCTTCATCGCATCGCCTTCTTTCCCTCCAATCGACGACGATTCTCAGATTGCCTTTCTCTTTGCAGTTCCTTTTTCTCTGCCTCGGAAGCTCAGATGGAGCAACGGGATTCTGTGGACAAGGAGTCTCTTATTGGTACTTGCAATAGTTATATGAATGTGGATGGTGTTTCTCCAAGTGGAAATATTTCCACGCCAACTATAAATAATGATGAAGCTAGAAGAACCAATGAGAATACAAGTGACAAACCTAATATTCCACATATATACAGACAAGACATTGTAAAAAGCAAAGGCAGTGGTATGATAGGGATTGTGACTGAAGTTGCTGGTGATGCTGATTCAGATAGTGACATTACTGATGATGAAGATGACGACGATGACGGCGAGGATGGTGTTGATGACGATGGTTGTGATGATTTTGATGGCGATGGTGAGAAAGAAGGTCAAAACAAGGAAAATAATGGCGATGACAGTAATGCTAAAAATTCCAACAAAGATAACTATAAGAGCCAACCCCTTCCTGTTGATGAAGTTCGAGTCCTTTGGATGGATGAAAGTGAGACAACTCAGAATGTTAATGATTTAACAGTTATAGATAGGGGATTTTTACACGGTGATTTTGTTGCTGCTGTTTCCGATCCAACAGGACAAGCAGGTGTTGTGGTAGATGTAAATATCTCTGTTGATTTGTTGGTTCCCGATGGATCAATTATGAAAGATATATCATCTAAAGACTTGAAACGTGTCAGAGATTTCACTGTGGGGGATTATGTCGTCCTCGGTCCATGGTTGGGTAGAATTGATGATGTCTTGGATAATGTGACTGTGATGTTTGATGATGGCTCCAAGTGTAAAGTCACAAAGGCAGAGCCATTGCGGCTCAAACCAGTTTCTAAGAATATCCTTGAAGATGCAAACTTTCCTTACTATCCTGGGCAGCGTGTAAGGGCGAGTTCTTCAACAGTTTTTAAGAATTCTAAATGGCTTTCTGGTTTATGGAAACCTAATCGCATGGAAGGTACTGTCACTAAAGTGACAGTTGGATCAGTGTTTATTTATTGGATAGCATCTGCTGGATATGGACCAGATTCTTCTACCACCCCAGCTGAAGAACAGACTCCAAAGAACTTGAGACTATTAACTTGTTTCTCACATGCGAATTGGCAATTGGGTGACTGGTGTCTTCTTCCCTCATCATTCTCCTCTGGCTCAACTAAGGACTCATCGCAAACGGAGCTTCCTGATTCAGTGACCAATACATTAGATTCTGCACAAACGGTGGGTGCATGTGATTCAGAGGATACTGTGCTAGATGAATTAAGTGGGACAACTGAATCCACTGATCTTGATTCCATTAGTGCATGTGATGGAAATTATAGGAATCCAGAATGTAATATTTTGCCCGAATCCAGTAGTTCTAGAGCTCCAAAGGAGAATACCCATGAGACTTGGCCTCTACATCGGAAAAAAATTCGTAAAGTTGTCGTCAGGAGAGATAAGAAGGCTAGAAAGAAAGAGGAAAACTTTGAGAGGGCCCTTTTAATTATCAATACTAAGACAAAGGTTGATGTTGCATGGCAGGATGGCCAGACAGAACTTGAACTGGATTCAACAAGCTTGATTCCAATTGATAATCCAGGTGATCATGAATTTGTTCCTGAACAGTATGTGGTTGAGAAGGCCTCTGATAATGATGATGATGTTACCGAAAGCAGGCGAGTTGGAGTTGTGAAAAGTGTTCATGCAAAGGAGCGAACAGCCTGTGTAAGGTGGTTAAAACCAGTATCCAGGGCAGAGGACCCTCGAGAATTTGACATAGAGGAAATTGTTAGTGTGTATGAGCTAGAGGGACATCCGGATTATGACTACTGTTATGGTGACGTGGTTGTTCGATTGTCACCTATTTCTGATTCTACAGAAGCAATGTCTTCTGAAAACAATACTGAGGAATTAAATGGGGAAAGTTCAACAAATGGGGTGATGAGTTGTACAGAAAAGGGTTCAGGAAGCCCGAAAATAGAAGATCCATCATGTAGTGATGATTGCATCGACTTCTCAGATCTCTCTTGGGTTGGAAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACTTGGGCTAATGGCATGGTTTCAACGGTTGGCCCTCAAGCAATTTATGTTGTTGGTCGAGATGATGACGATGAATCAATTGCTGCTGGAAGTGAAGTAAGTAATGGTGCAGCAAGTTGGGAAACAGTTGATGATGATGAAATAGATTCTGTTGAGAATGCTAAGGAGGATATTGAGCTGCAGGATACTGGTGTCAATTCTGAAGAGGAAGATAGTGAACAGAATAATTCAGGAAGAAATCTAGCTCTTTCTGTTCCACTTGCTGCACTTCGATTTGTTACTAGATTGGCTGCTGGAATATTCTCACGAGGACCAAGAAATCCAGATTCAATGGATTTGGATTCCCACAGTGAAAGTGAGACTCAATCCTTGGAAATTCAAGCTTCAGAGGAAAAAGATTCTGGGCTTCAGTCTAGCTCTCTTAAATCCAATTCTTTTGATGCTTCTGATATGAACTCTGATTGTGGAAGAGGAGAGGATGATGATGCTTCAGAGTCATCAAAAGTGTTGGAATCAGCAGAAGCATCAAGCAACCTGAGGACTGCAGAATCCGATGGTTCAGCATGCCATGAGGATGGCACCTGCAGCTTCAAAGGCTTTGATATAGCCAAAGATCCTTTGGACCATTATTTTCTTGGTACAAATGGACAAACTAATAATGGAAGGAAATGGCTCAAGAAAATCCAGCAAGATTGGAGTATCCTCCAGAACAATCTGCCAGATGGGATATACGTTCGAGTCTACGAGGATCGAATGGACCTTTTAAGAGCAGTCATTGTTGGGGCCTATGGGACTCCTTACCAAGATGGCCTCTTCTTCTTTGATTTTCATCTCCCACCAGAGTATCCTGACGTTCCACCATCAGCATATTATCATTCAGGTGGGTGGCGGATAAATCCCAATCTTTATGAGGAAGGCAAAGTCTGCCTTAGCCTTTTGAATACTTGGACTGGCAGAGGAAATGAAGTATGGGATCCAAAGTCCTCTAGCATCCTTCAAGTGTTGGTCTCGCTTCAGGGATTAGTGTTGAATTCTAAACCATATTTTAATGAAGCTGGATATGACAAGCAAATTGGGACAGCCGAAGGAGAGAAAAATTCTTTGTCGTACAATGAAAACACTTTCTTATTAAGCTGCAAGACAATAATGTATCTTATGAGGAAGCCTCCCAAGGACTTTGAAGAACTCGTTAAAGAACATTTCAGGAGGCGTGGATATTACATCCTGAAGGCCTGTGATTCATACATGAAAGGTCACTTAATTGGCTCCCTTACAGAAGATGCCTCCATAAGAGAAGAAAGCGATCCTAACTCCACGTCAGTCGGATTTAAGCTTATGCTCGCTAAGATTGTTCCGAAGCTCTTCTCGTCGTTGAACGAAGTCGGAGCTGATTGTCACGATTTTAAGCATTTTCAACAGTTGTAGCATAAAAAAAACTCCAAATGACAAGTTATCATTTACATACAGCTGACAAGGTAATTTTTTCTTTTTTCTATTCATTCCCATTCATGTTAAAGTCTTCTTTTCTTTACCCATTATGATTAATGATGCACCTTGTTTATAAGTTTGAACTGGGCCCTCATGCCTTTGAAAGCTTCATAGAATTCTAGGAGCTGGGTTTTTGGGACTACTCTCTGGTCTCTGTGATCCATTTTTTTTCCTCTAAAAAAAAAAGAACGAAAGAAAGATTACATTGCAAGTTAGGAATTAGTTGATCTTGTTCTGTCTTAGGTTGGTTTGTGCCTAAAATGGACTTTATTGAGATTTTTTTTGTTTTACTGCGGTTAAAGAATTGCTCAAAGAAGAGTAAACAAGTATGATATTTTGTGCTGC

Coding sequence (CDS)

ATGGAGCAACGGGATTCTGTGGACAAGGAGTCTCTTATTGGTACTTGCAATAGTTATATGAATGTGGATGGTGTTTCTCCAAGTGGAAATATTTCCACGCCAACTATAAATAATGATGAAGCTAGAAGAACCAATGAGAATACAAGTGACAAACCTAATATTCCACATATATACAGACAAGACATTGTAAAAAGCAAAGGCAGTGGTATGATAGGGATTGTGACTGAAGTTGCTGGTGATGCTGATTCAGATAGTGACATTACTGATGATGAAGATGACGACGATGACGGCGAGGATGGTGTTGATGACGATGGTTGTGATGATTTTGATGGCGATGGTGAGAAAGAAGGTCAAAACAAGGAAAATAATGGCGATGACAGTAATGCTAAAAATTCCAACAAAGATAACTATAAGAGCCAACCCCTTCCTGTTGATGAAGTTCGAGTCCTTTGGATGGATGAAAGTGAGACAACTCAGAATGTTAATGATTTAACAGTTATAGATAGGGGATTTTTACACGGTGATTTTGTTGCTGCTGTTTCCGATCCAACAGGACAAGCAGGTGTTGTGGTAGATGTAAATATCTCTGTTGATTTGTTGGTTCCCGATGGATCAATTATGAAAGATATATCATCTAAAGACTTGAAACGTGTCAGAGATTTCACTGTGGGGGATTATGTCGTCCTCGGTCCATGGTTGGGTAGAATTGATGATGTCTTGGATAATGTGACTGTGATGTTTGATGATGGCTCCAAGTGTAAAGTCACAAAGGCAGAGCCATTGCGGCTCAAACCAGTTTCTAAGAATATCCTTGAAGATGCAAACTTTCCTTACTATCCTGGGCAGCGTGTAAGGGCGAGTTCTTCAACAGTTTTTAAGAATTCTAAATGGCTTTCTGGTTTATGGAAACCTAATCGCATGGAAGGTACTGTCACTAAAGTGACAGTTGGATCAGTGTTTATTTATTGGATAGCATCTGCTGGATATGGACCAGATTCTTCTACCACCCCAGCTGAAGAACAGACTCCAAAGAACTTGAGACTATTAACTTGTTTCTCACATGCGAATTGGCAATTGGGTGACTGGTGTCTTCTTCCCTCATCATTCTCCTCTGGCTCAACTAAGGACTCATCGCAAACGGAGCTTCCTGATTCAGTGACCAATACATTAGATTCTGCACAAACGGTGGGTGCATGTGATTCAGAGGATACTGTGCTAGATGAATTAAGTGGGACAACTGAATCCACTGATCTTGATTCCATTAGTGCATGTGATGGAAATTATAGGAATCCAGAATGTAATATTTTGCCCGAATCCAGTAGTTCTAGAGCTCCAAAGGAGAATACCCATGAGACTTGGCCTCTACATCGGAAAAAAATTCGTAAAGTTGTCGTCAGGAGAGATAAGAAGGCTAGAAAGAAAGAGGAAAACTTTGAGAGGGCCCTTTTAATTATCAATACTAAGACAAAGGTTGATGTTGCATGGCAGGATGGCCAGACAGAACTTGAACTGGATTCAACAAGCTTGATTCCAATTGATAATCCAGGTGATCATGAATTTGTTCCTGAACAGTATGTGGTTGAGAAGGCCTCTGATAATGATGATGATGTTACCGAAAGCAGGCGAGTTGGAGTTGTGAAAAGTGTTCATGCAAAGGAGCGAACAGCCTGTGTAAGGTGGTTAAAACCAGTATCCAGGGCAGAGGACCCTCGAGAATTTGACATAGAGGAAATTGTTAGTGTGTATGAGCTAGAGGGACATCCGGATTATGACTACTGTTATGGTGACGTGGTTGTTCGATTGTCACCTATTTCTGATTCTACAGAAGCAATGTCTTCTGAAAACAATACTGAGGAATTAAATGGGGAAAGTTCAACAAATGGGGTGATGAGTTGTACAGAAAAGGGTTCAGGAAGCCCGAAAATAGAAGATCCATCATGTAGTGATGATTGCATCGACTTCTCAGATCTCTCTTGGGTTGGAAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACTTGGGCTAATGGCATGGTTTCAACGGTTGGCCCTCAAGCAATTTATGTTGTTGGTCGAGATGATGACGATGAATCAATTGCTGCTGGAAGTGAAGTAAGTAATGGTGCAGCAAGTTGGGAAACAGTTGATGATGATGAAATAGATTCTGTTGAGAATGCTAAGGAGGATATTGAGCTGCAGGATACTGGTGTCAATTCTGAAGAGGAAGATAGTGAACAGAATAATTCAGGAAGAAATCTAGCTCTTTCTGTTCCACTTGCTGCACTTCGATTTGTTACTAGATTGGCTGCTGGAATATTCTCACGAGGACCAAGAAATCCAGATTCAATGGATTTGGATTCCCACAGTGAAAGTGAGACTCAATCCTTGGAAATTCAAGCTTCAGAGGAAAAAGATTCTGGGCTTCAGTCTAGCTCTCTTAAATCCAATTCTTTTGATGCTTCTGATATGAACTCTGATTGTGGAAGAGGAGAGGATGATGATGCTTCAGAGTCATCAAAAGTGTTGGAATCAGCAGAAGCATCAAGCAACCTGAGGACTGCAGAATCCGATGGTTCAGCATGCCATGAGGATGGCACCTGCAGCTTCAAAGGCTTTGATATAGCCAAAGATCCTTTGGACCATTATTTTCTTGGTACAAATGGACAAACTAATAATGGAAGGAAATGGCTCAAGAAAATCCAGCAAGATTGGAGTATCCTCCAGAACAATCTGCCAGATGGGATATACGTTCGAGTCTACGAGGATCGAATGGACCTTTTAAGAGCAGTCATTGTTGGGGCCTATGGGACTCCTTACCAAGATGGCCTCTTCTTCTTTGATTTTCATCTCCCACCAGAGTATCCTGACGTTCCACCATCAGCATATTATCATTCAGGTGGGTGGCGGATAAATCCCAATCTTTATGAGGAAGGCAAAGTCTGCCTTAGCCTTTTGAATACTTGGACTGGCAGAGGAAATGAAGTATGGGATCCAAAGTCCTCTAGCATCCTTCAAGTGTTGGTCTCGCTTCAGGGATTAGTGTTGAATTCTAAACCATATTTTAATGAAGCTGGATATGACAAGCAAATTGGGACAGCCGAAGGAGAGAAAAATTCTTTGTCGTACAATGAAAACACTTTCTTATTAAGCTGCAAGACAATAATGTATCTTATGAGGAAGCCTCCCAAGGACTTTGAAGAACTCGTTAAAGAACATTTCAGGAGGCGTGGATATTACATCCTGAAGGCCTGTGATTCATACATGAAAGGTCACTTAATTGGCTCCCTTACAGAAGATGCCTCCATAAGAGAAGAAAGCGATCCTAACTCCACGTCAGTCGGATTTAAGCTTATGCTCGCTAAGATTGTTCCGAAGCTCTTCTCGTCGTTGAACGAAGTCGGAGCTGATTGTCACGATTTTAAGCATTTTCAACAGTTGTAG

Protein sequence

MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTESTDLDSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCHDFKHFQQL
Homology
BLAST of Tan0001044 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 1299.6 bits (3362), Expect = 0.0e+00
Identity = 710/1136 (62.50%), Postives = 854/1136 (75.18%), Query Frame = 0

Query: 23   DGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQDIVKSKGSGMIGIVTEVAGDAD 82
            D    S N+   +  +D     +   S+ PN   IYRQDIVK+K +G +G+V+EVAGD+D
Sbjct: 6    DDPGTSTNVGVDSSVDDSMASLSICDSEHPN---IYRQDIVKNKKTGSVGVVSEVAGDSD 65

Query: 83   SDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNKENNGDDSNAKNSNKDNYKSQPL 142
            SDSDI+D+E+DDDD ED  DDD  +D + +G+K  +    NGD     +    NYK   L
Sbjct: 66   SDSDISDEEEDDDDDEDNDDDD--EDVE-EGKKASEENVVNGDGEKKADG---NYKCGAL 125

Query: 143  PVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSDPTGQAGVVVDVNISVDLLVP 202
              D++RVLWMD +E  Q++ND+TVIDRGFLHGD+VA+ S+PTGQ GVVVDVNISVDLL P
Sbjct: 126  EGDQIRVLWMDNTEPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAP 185

Query: 203  DGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLR 262
            DGSI KDIS+K+LKRVRDF VGDYVV GPWLGRIDDVLDNVTV+FDDGS CKV + EPLR
Sbjct: 186  DGSIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLR 245

Query: 263  LKPVSKNIL-EDANFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRMEGTVTKVTVGSVFI 322
            LKP+ KN L EDANFPYYPGQRV+ASSS+VFKNS+WLSGLWKPNR+EGTVTKVT GS+F+
Sbjct: 246  LKPIPKNNLEEDANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFV 305

Query: 323  YWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLGDWCLLPSSFSSGST---KDSS 382
            YWIASAG+GPDSS +P EEQ P NL LL+CF+HANWQ+GDWCLLPS   S +    K  S
Sbjct: 306  YWIASAGFGPDSSVSPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVS 365

Query: 383  QTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTE-----STDLDSISACDGNYRNPEC 442
            +  L DS     D  Q +G  D ED V DE+SG  E     +  L  +++ D   RNP  
Sbjct: 366  KLRLYDS---QADRQQKIGR-DLED-VQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSV 425

Query: 443  NILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENFERALLIINTKTKVDV 502
            +           KE  HE WPLHRKKIRK+V+R+DKK +KKEE+FE+ALL++N++T+VDV
Sbjct: 426  S-----------KEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALLVVNSRTRVDV 485

Query: 503  AWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKE 562
            +WQDG  E   ++ +LIPI+ PGDHEFV EQYVVEK SD+ D+ TE RR GVVK+V+AK+
Sbjct: 486  SWQDGTIECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKD 545

Query: 563  RTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVRLSPISDSTEAMSS 622
            RTA VRWL P+ RAE+PREF+ EEIVSVYELEGHPDYDYCYGDVVVRLSPI+ +  A S 
Sbjct: 546  RTASVRWLNPLRRAEEPREFEKEEIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSP 605

Query: 623  ENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWVGNITGLKNGDIEV 682
             N+ EE   +   NG    +E    +  + D   ++   D S LSWVGNITGLK+GDIEV
Sbjct: 606  GNSFEEATQQD--NGYQD-SESHQEAKILVDKEENEPSTDLSKLSWVGNITGLKDGDIEV 665

Query: 683  TWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENAKEDI-E 742
            TWA+G +STVGP A+YVVGRDDDDES+A  SE S+ AASWET++DD+  + E  +ED+  
Sbjct: 666  TWADGTISTVGPHAVYVVGRDDDDESVAGESETSD-AASWETLNDDDRGAPEIPEEDLGR 725

Query: 743  LQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGPRNPDSMDLDSHSES 802
                  NS+ +   +N+SGRN AL++PLAA+ FVTRLA+GIFSR  ++ DS   D   E+
Sbjct: 726  SSSIEGNSDADIYAENDSGRNGALALPLAAIEFVTRLASGIFSRARKSVDSSSSDYTVEN 785

Query: 803  ETQSLE-IQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASESSKVLESAEASSN 862
              +  E    S+E DS    S  K N  D      +C   E      ++K + S E S+ 
Sbjct: 786  VYKQAESTNPSDETDSLDDPSPSKVNVTD------NC---ESKGTQANAKNILSGETSTF 845

Query: 863  LRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLKKIQQDWSILQNN 922
            L   + D     E  +CSF+ FDI++DPLDH+FLG +GQ    R+W KK+ QDW ILQNN
Sbjct: 846  LE--DEDKPVPSEGDSCSFRRFDISQDPLDHHFLGVDGQKTKERQWFKKVDQDWKILQNN 905

Query: 923  LPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRI 982
            LPDGI+VR YEDRMDLLRAVIVGA+GTPYQDGLFFFDFHLP +YP VPPSAYYHSGGWR+
Sbjct: 906  LPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRL 965

Query: 983  NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQI 1042
            NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ+
Sbjct: 966  NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQV 1025

Query: 1043 GTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKG 1102
            GTAEGEKNSL YNENTFLL+CKT+MYLMRKPPKDFEEL+K+HFR+RGYYILKACD+YMKG
Sbjct: 1026 GTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKG 1085

Query: 1103 HLIGSLTEDAS-IREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCHDFKHFQQ 1147
            +LIGSLT+DAS I E S  NSTSVGFKLMLAKI PKLFS+L+EVGADC++F+H QQ
Sbjct: 1086 YLIGSLTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEFQHLQQ 1101

BLAST of Tan0001044 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-170
Identity = 372/991 (37.54%), Postives = 538/991 (54.29%), Query Frame = 0

Query: 156  ETTQNVNDLTVIDRGFLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDL 215
            ET   +++    +RGF++GD V + ++P+GQ+G V+++++ V+L    G IMK++ +K L
Sbjct: 41   ETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRL 100

Query: 216  KRVRDFTVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDAN 275
            +++R  ++ DYV+ GPW+GR+D +++ V+V  DDG+  +V      +L  +  N+LED+ 
Sbjct: 101  QKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQ 160

Query: 276  FPYYPGQRVRASSSTVFKNSKWLSGLWKPNRMEGTVTKVTVGSVFIYWIAS-AGYGPDSS 335
            + YYPGQRV+   +   +++ WL G W+  ++ GTV  V  G V++ W+AS    G  + 
Sbjct: 161  YSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNL 220

Query: 336  TTPAEEQTPKNLRLLTCFSHANWQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQ 395
            T P   Q P++L LL C SHA+WQLGDWC+LP S    S  D ++ + P+     L+   
Sbjct: 221  TAPQALQNPESLTLLPCVSHASWQLGDWCILPGS----SHCDIAERQTPNVAAYNLN--- 280

Query: 396  TVGACDSEDTVLDELSGTTESTDLDSISACDGNYRNPECNILPESSSSRAPKENTHETWP 455
                                                 EC                H+T+ 
Sbjct: 281  -------------------------------------EC----------------HKTF- 340

Query: 456  LHRKKIRKVVVRRDKKARKKEENFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDN 515
                       ++      +    +   +I  TK KV V WQDG   L +DS  L+P+  
Sbjct: 341  -----------QKGFNRNMQNSGLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGA 400

Query: 516  PGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSR-AEDPREF 575
               H+F PEQ+VVEK + N      S++ GVVK+V+AKE+T  V+W   V + A    + 
Sbjct: 401  VNAHDFWPEQFVVEKETCN------SKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDE 460

Query: 576  DIEEIVSVYELEGHPDYDYCYGDVVVRLSPIS--DSTEAMSSENNTEELNGESSTNGVMS 635
             +EEIVS YEL  HPD+ +C+ DVVV+L P    D           + L  ES  +G   
Sbjct: 461  VMEEIVSAYELLEHPDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKHLLTESDYSGAYF 520

Query: 636  CTEKGSGSPKIEDPSCSDDCIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVV 695
                                     LS +G +TG KNG ++V WANG  S V P  I+ +
Sbjct: 521  -------------------------LSSIGVVTGFKNGSVKVKWANGSTSKVAPCEIWKM 580

Query: 696  GRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSG 755
             R +   S    SE S    S +    DE  S       ++L   G +  E   E +   
Sbjct: 581  ERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVGESCNENIPECS--- 640

Query: 756  RNLALSVPLAALRFVTRLAAGIFSRGPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQS 815
               +  +P AA+ F+T LA+ +F  G +   S+ + SHS           SE++      
Sbjct: 641  ---SFFLPKAAIGFITNLASSLF--GYQGSTSV-ISSHSRCND-------SEDQSDSEVL 700

Query: 816  SSLKSNSFDASDMNSDCGRGEDDDASESSKV-LESAEASSNLRTAESDGSACHEDGTCSF 875
                + S+D S+ NS    GE D  +    + +E    +  L +   + S         F
Sbjct: 701  VQETAESYDNSETNS----GEVDMTTTMVNIPIEGKGINKTLDSTLLENSR----NQVRF 760

Query: 876  KGFDIAKDPLDHYFLGTN---GQTNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDL 935
            + FD+  D  DH+FL ++    Q+   + W+KK+QQ+WS L+ NLP+ IYVRV E+RMDL
Sbjct: 761  RQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDL 820

Query: 936  LRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 995
            LRA +VGA GTPY DGLFFFD  LPP+YP  PP  +YHSGG R+NPNLYE G+VCLSLLN
Sbjct: 821  LRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLN 880

Query: 996  TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1055
            TW+G G EVW+  SSSILQ+L+S Q LVLN KPYFNEAGYDKQ+G AEGEKNS+SYNEN 
Sbjct: 881  TWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENA 899

Query: 1056 FLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREES 1115
            FL++CK+++ ++RKPPK FE LVK+HF  R  ++L AC +YM+G  +GS    A+++  S
Sbjct: 941  FLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS---SANLQGNS 899

Query: 1116 DPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1139
              NST  GFK+ML+K+ PKL  + +E+G DC
Sbjct: 1001 TTNST--GFKIMLSKLYPKLLEAFSEIGVDC 899

BLAST of Tan0001044 vs. ExPASy Swiss-Prot
Match: Q6ZPJ3 ((E3-independent) E2 ubiquitin-conjugating enzyme UBE2O OS=Mus musculus OX=10090 GN=Ube2o PE=1 SV=3)

HSP 1 Score: 297.4 bits (760), Expect = 7.2e-79
Identity = 307/1172 (26.19%), Postives = 506/1172 (43.17%), Query Frame = 0

Query: 57   IYRQDIV--KSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFDGDGE 116
            ++  D+V  + +GS   G+V  + G+ DSDS     E DD    DG    GC +  G G 
Sbjct: 55   LFSHDLVSGRYRGSVHFGLVRLIHGE-DSDS-----EGDD----DGRGSSGCSEAGGAGH 114

Query: 117  KEGQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVND--LTVIDRGFL 176
            +EG                    ++ PL    VRV W  E    Q+V +  L + DR  +
Sbjct: 115  EEG--------------------RASPLRRGYVRVQWYPEG-VKQHVKETKLKLEDRSVV 174

Query: 177  HGDFVAAVSDPTGQAGVVVDVNISVDL-LVPDGSIMKDISSKDLKRVRDFTVGDYVVLGP 236
              D V  +     Q G V+DVNI   + L+    I+  ++SKDL+ +  F  GDY+    
Sbjct: 175  PRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDC 234

Query: 237  WLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILE-----DANFPYYPGQRVRA 296
            WLG++ D+ + + +   +G++C +   +  +L  V  ++ +     D ++ +YPGQ V  
Sbjct: 235  WLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQ-VLI 294

Query: 297  SSSTVFKNSKWLSGLWKP-----NRMEGTVTKVTVGSVFIYWIASA--GYGPDSSTTPAE 356
              + +F + +WLSG+ KP     ++    V +V V  + + WI  +    G DS + P  
Sbjct: 295  GPAKIFSSVQWLSGV-KPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPS 354

Query: 357  EQTPKNL---RLLTCFSHANWQLGDWCL-------------------------------- 416
              T +NL   + L CF HA  QLG+ CL                                
Sbjct: 355  IITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKV 414

Query: 417  -------------------LPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGAC---DSE 476
                                P   S        +      + +     Q  G+    + +
Sbjct: 415  KRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQ 474

Query: 477  DTVLDELSGTTE------------------STDLDSISACDGNYRNPECNILPESSSSRA 536
            D   +EL  T E                    D D  +A D +  +   +    ++SS++
Sbjct: 475  DEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQS 534

Query: 537  PK-ENTHETWPLHRKKIRKVVVRRDKKARKKEENFER-ALLIINTKTKVDVAWQDGQTEL 596
                   ++ PL  K +++   R+  K  +  +  +R A+ ++ T T  DV WQDG  E 
Sbjct: 535  GSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVEC 594

Query: 597  ELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKERTACVRWLK 656
             + S  L P+ +  ++EF P  +VV+K   +  D       GVV+S     RT  V+W K
Sbjct: 595  NIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAV---YGVVQSGDHVGRTCMVKWFK 654

Query: 657  PVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVRLSPISDSTEAMSSENNTEELNG 716
                 +D      EE VSVY++  HPD+ +   D+V+R+              NTE+   
Sbjct: 655  LRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRI-------------GNTED--- 714

Query: 717  ESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWVGNITGLK-NGDIEVTWANGMVS 776
                             PK ++PS             VG +  +  +  +EV WA+   +
Sbjct: 715  --------------GALPKEDEPS-------------VGQVARVDVSSKVEVVWADNSKT 774

Query: 777  TVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENAKED-----IELQDT 836
             + PQ +Y +  + ++    +    S+GA+S E  DD +    +N   D     IE    
Sbjct: 775  IILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAA 834

Query: 837  GVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLA---AGIFSRGPRN--PDSMDLDSHSE 896
             + +E+  + + + G  ++     AA++    +A   AG+  +  ++  P S      + 
Sbjct: 835  ILPAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAI 894

Query: 897  SETQSLEIQASEEKDSGLQSSSL---------KSNSFDASDMNSDCGRGEDDDA---SES 956
               +SL+    E+  +G  +S           K    D   +  +  +  D+ A    E 
Sbjct: 895  KILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEK 954

Query: 957  SKVLESAEASSNLRTAESDGSA---------CHEDGTCSFKGFDIAKD---------PLD 1016
             + +   E        +S   A         C + G      F  AK          P +
Sbjct: 955  MEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSN 1014

Query: 1017 HYFLGTNGQTNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQ 1076
            H F     Q    +K+   ++++ ++L  +LPDGI V+ +EDRMDL  A+I G   TPY+
Sbjct: 1015 HSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYE 1074

Query: 1077 DGLFFFDFHLPPEYPDVPPS-AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPK 1093
            DGL+ FD  LP  YP VPP   Y      R+NPNLY+ GKVC+SLL TW G+G E W  K
Sbjct: 1075 DGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSK 1134

BLAST of Tan0001044 vs. ExPASy Swiss-Prot
Match: Q9C0C9 ((E3-independent) E2 ubiquitin-conjugating enzyme OS=Homo sapiens OX=9606 GN=UBE2O PE=1 SV=3)

HSP 1 Score: 292.4 bits (747), Expect = 2.3e-77
Identity = 309/1194 (25.88%), Postives = 519/1194 (43.47%), Query Frame = 0

Query: 57   IYRQDIV--KSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDG-EDGVDDDGCDDFDGDG 116
            ++  D+V  + +GS   G+V  + G           ED D +G E+G    GC +  G G
Sbjct: 60   LFSHDLVSGRYRGSVHFGLVRLIHG-----------EDSDSEGEEEGRGSSGCSEAGGAG 119

Query: 117  EKEGQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVND--LTVIDRGF 176
             +EG                    ++ PL    VRV W  E    Q+V +  L + DR  
Sbjct: 120  HEEG--------------------RASPLRRGYVRVQWYPEG-VKQHVKETKLKLEDRSV 179

Query: 177  LHGDFVAAVSDPTGQAGVVVDVNISVDL-LVPDGSIMKDISSKDLKRVRDFTVGDYVVLG 236
            +  D V  +     Q G V+DVNI   + L+    I+  ++SKDL+ +  F  GDY+   
Sbjct: 180  VPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYD 239

Query: 237  PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILE-----DANFPYYPGQRVR 296
             WLG++ D+ + + +   +G++C +   +  +L  V  ++ +     D ++ +YPGQ V 
Sbjct: 240  CWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQ-VL 299

Query: 297  ASSSTVFKNSKWLSGLWKP-----NRMEGTVTKVTVGSVFIYWIASA--GYGPDSSTTPA 356
               + +F + +WLSG+ KP     ++    V +V V  + + WI  +    G DS + P 
Sbjct: 300  IGPAKIFSSVQWLSGV-KPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPP 359

Query: 357  EEQTPKNL---RLLTCFSHANWQLGDWCLL------------------------------ 416
               T +NL   + L CF HA  QLG+ CL                               
Sbjct: 360  SVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKK 419

Query: 417  -----------------------------PSSFSSGSTKDSSQT----ELPDSVTNTLDS 476
                                         P       TK  +++    E PD   + ++ 
Sbjct: 420  VKRLLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVE- 479

Query: 477  AQTVGACDSEDT-------VLDELSGT---TESTDLDSISACDGNYRNPECNILPESSSS 536
             Q  GA +  +        +L E       +   D D  +A D +  +   +    ++SS
Sbjct: 480  MQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSS 539

Query: 537  RAPKENTH-ETWPLHRKKIRKVVVRRDKKARKKEENFER-ALLIINTKTKVDVAWQDGQT 596
            ++    +  ++ PL  K +++   R+  K  +  +  +R A+ ++ T T  DV WQDG  
Sbjct: 540  QSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSV 599

Query: 597  ELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKERTACVRW 656
            E  + S  L P+ +  ++EF P  +VV+K   +  D       GVV+S     RT  V+W
Sbjct: 600  ECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAV---YGVVQSGDHIGRTCMVKW 659

Query: 657  LKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVRLSPISDSTEAMSSENNTEEL 716
             K     +D      EE VSVY++  HPD+ +   D+V+R+              NTE+ 
Sbjct: 660  FKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRI-------------GNTED- 719

Query: 717  NGESSTNGVMSCTEKGSGSPKIED-PSCSDDCIDFSDLSWVGNITGLK-NGDIEVTWANG 776
                             G+P  ED PS             VG +  +  +  +EV WA+ 
Sbjct: 720  -----------------GAPHKEDEPS-------------VGQVARVDVSSKVEVVWADN 779

Query: 777  MVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENA---KEDIELQD 836
              + + PQ +Y +  + ++    +    ++GA+S E  DD +    +N     E  ++++
Sbjct: 780  SKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEE 839

Query: 837  TGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLA---AGIFSRGPRN--PDSMDLDSHS 896
              +   E+     + G  ++     AA++    +A   AG+  +  ++  P S      +
Sbjct: 840  PPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEA 899

Query: 897  ESETQSLEIQASEEKDSGLQSSSL---------KSNSFDASDMNSDCGRGEDDDA---SE 956
                +SL+    E+  +G  +S           K    D   +  +  +  D+ A    E
Sbjct: 900  IKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEE 959

Query: 957  SSKVLESAEASSNLRTAESDGSA---------CHEDGTCSFKGFDIAKD---------PL 1016
              + +   E   +    +S   A         C + G      F  AK          P 
Sbjct: 960  KMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPS 1019

Query: 1017 DHYFLGTNGQTNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPY 1076
            +H F     Q    +K+   ++++ ++L  +LP+GI V+ +EDRMDL  A+I G   TPY
Sbjct: 1020 NHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPY 1079

Query: 1077 QDGLFFFDFHLPPEYPDVPPS-AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDP 1114
            +DGL+ FD  LP  YP VPP   Y      R+NPNLY+ GKVC+SLL TW G+G E W  
Sbjct: 1080 EDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTS 1139

BLAST of Tan0001044 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.3e-75
Identity = 136/257 (52.92%), Postives = 177/257 (68.87%), Query Frame = 0

Query: 870  FKGFDIAKDPLDHYFLGTNGQTN--NGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDL 929
            FK FDI +D  DH++  + G T+  + + W K+IQ +W IL+ +LP+ I+VR YE RMDL
Sbjct: 305  FKRFDIVEDFSDHHY-ASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDL 364

Query: 930  LRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 989
            LRAVI+GA GTPY DGLFFFD   P  YP  PP  +YHSGG RINPNLY  GKVCLSLL 
Sbjct: 365  LRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLG 424

Query: 990  TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1049
            TW+G   E W P +S++LQVLVS+QGL+LN KPYFNE GY++  G+A GE  S +Y+ENT
Sbjct: 425  TWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENT 484

Query: 1050 FLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREES 1109
            F+LS KT++Y MR+PPK FE+    HF    + +LKAC++Y  G   G L + A   EE+
Sbjct: 485  FILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEEN 544

Query: 1110 DPNSTSVGFKLMLAKIV 1125
                +S+ F+  +A  V
Sbjct: 545  SAGMSSLKFRTDVATFV 560

BLAST of Tan0001044 vs. NCBI nr
Match: XP_022986085.1 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 [Cucurbita maxima])

HSP 1 Score: 2078.5 bits (5384), Expect = 0.0e+00
Identity = 1056/1147 (92.07%), Postives = 1094/1147 (95.38%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+ SVDKESLIGT NSY N DGVS S N+   TINNDE RRTNENTSDKP++PHIYRQ
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTTINNDETRRTNENTSDKPSVPHIYRQ 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNK 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITDDED+D+DGEDG D  GCDD DGDGEKEGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDGEDGEDGGGCDDDDGDGEKEGQNK 120

Query: 121  ENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180
            EN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  ENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRGFLHGDFVAAV 180

Query: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240
            SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL
Sbjct: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240

Query: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSG 300
            DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRV+ASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWLSG 300

Query: 301  LWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360
            LWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG
Sbjct: 301  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360

Query: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTESTDLDS 420
            DWCLLPSSFSSG TK+SSQT   DSVTN+LDS Q    CDSED VL+ELSGTTESTDLDS
Sbjct: 361  DWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELSGTTESTDLDS 420

Query: 421  ISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENFER 480
            +SACDGNYRNPECNILPESS+SRA KE +HETWPLHRKKIRKVV+RRDKKARKKEENFER
Sbjct: 421  LSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 481  ALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTES 540
            ALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKASDNDDD++ES
Sbjct: 481  ALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDISES 540

Query: 541  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVR 600
            RRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDYDYCYGDVVVR
Sbjct: 541  RRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVVVR 600

Query: 601  LSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWV 660
            LSP+SDS E++S  NNTEEL  +SSTN +M+CTEK SGS KIEDPSCSDDCIDFSDLSWV
Sbjct: 601  LSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLSWV 660

Query: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720
            GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE
Sbjct: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720

Query: 721  IDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGPRN 780
             DSV+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLAAGIFSRGPRN
Sbjct: 721  RDSVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSRGPRN 780

Query: 781  PDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASESS 840
            P+ MD+DSHSESETQSLEIQA EEKDSGLQSSSLKSNSFDASDMNSDCGRGED+ ASE S
Sbjct: 781  PELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDNIASEPS 840

Query: 841  KVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLKK 900
            +VLESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTN+GRKWLKK
Sbjct: 841  EVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNSGRKWLKK 900

Query: 901  IQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 960
            IQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP
Sbjct: 901  IQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 960

Query: 961  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP 1020
            SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVLNSKP
Sbjct: 961  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVLNSKP 1020

Query: 1021 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYY 1080
            YFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFRRRGY+
Sbjct: 1021 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYF 1080

Query: 1081 ILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCHD 1140
            ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVGADC D
Sbjct: 1081 ILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLMSSLNEVGADCDD 1140

Query: 1141 FKHFQQL 1148
            FKHFQQL
Sbjct: 1141 FKHFQQL 1147

BLAST of Tan0001044 vs. NCBI nr
Match: KAG6571012.1 (putative ubiquitin-conjugating enzyme E2 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2073.5 bits (5371), Expect = 0.0e+00
Identity = 1056/1151 (91.75%), Postives = 1094/1151 (95.05%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+ SVDKESLIGT NSY N DGVS S N+   TINNDE RRTNENTSDKP+IPHIYRQ
Sbjct: 508  MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTTINNDETRRTNENTSDKPSIPHIYRQ 567

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITD----DEDDDDDGEDGVDDDGCDDFDGDGEKE 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITD    DED+D+DGEDG DDDGCDD DGDGEK 
Sbjct: 568  DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDEDEDGEDGEDDDGCDDDDGDGEKV 627

Query: 121  GQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDF 180
            GQNKEN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDF
Sbjct: 628  GQNKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRGFLHGDF 687

Query: 181  VAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRI 240
            VAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRI
Sbjct: 688  VAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRI 747

Query: 241  DDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSK 300
            DDVLDNVTVMFDDGSKCKVTKAEP+RLKPVSKNILEDANFPYYPGQRV+ASSSTVFKNSK
Sbjct: 748  DDVLDNVTVMFDDGSKCKVTKAEPMRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSK 807

Query: 301  WLSGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHAN 360
            WLSGLWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHAN
Sbjct: 808  WLSGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHAN 867

Query: 361  WQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTEST 420
            WQLGDWCLLPSSFSSG TK+SS+T   DSV N+LDS Q  G CDSED VL+ELSGTTEST
Sbjct: 868  WQLGDWCLLPSSFSSGLTKESSRTHRSDSVNNSLDSTQPEGVCDSEDVVLNELSGTTEST 927

Query: 421  DLDSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEE 480
            DLDS+SACDGNYRNPECNILPESS+SRA KE THETWPLHRKKIRKVV+RRDKKARKKEE
Sbjct: 928  DLDSLSACDGNYRNPECNILPESSNSRALKETTHETWPLHRKKIRKVVIRRDKKARKKEE 987

Query: 481  NFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDD 540
            NFERALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKASDNDDD
Sbjct: 988  NFERALLIINTKTKVDVAWQDGRTERGLDSTNLIPIDNPGDHEFVPEQYVVEKASDNDDD 1047

Query: 541  VTESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGD 600
            ++ESRRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDYDYCYGD
Sbjct: 1048 ISESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGD 1107

Query: 601  VVVRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSD 660
            VVVRLSP+SDS E+MS  N+TEEL  +SSTN +M+CTEK SGS KIEDPSCSDDCIDFSD
Sbjct: 1108 VVVRLSPVSDSIESMSLGNDTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSD 1167

Query: 661  LSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV 720
            LSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV
Sbjct: 1168 LSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV 1227

Query: 721  DDDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSR 780
            DDDE D V+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLAAGIFSR
Sbjct: 1228 DDDERDCVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSR 1287

Query: 781  GPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDA 840
            GPRNP+SMD+DSHSESETQSLEIQA EEKDSGLQSSSLKSNSFDASDMNSDCGRGED+ A
Sbjct: 1288 GPRNPESMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDNIA 1347

Query: 841  SESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRK 900
            SE S+V ESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTN+GRK
Sbjct: 1348 SEPSEVSESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNSGRK 1407

Query: 901  WLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYP 960
            WLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYP
Sbjct: 1408 WLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYP 1467

Query: 961  DVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVL 1020
            DVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVL
Sbjct: 1468 DVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVL 1527

Query: 1021 NSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRR 1080
            NSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFRR
Sbjct: 1528 NSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRR 1587

Query: 1081 RGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGA 1140
            RGY+ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVGA
Sbjct: 1588 RGYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLVSSLNEVGA 1647

Query: 1141 DCHDFKHFQQL 1148
            DC DFKHFQQL
Sbjct: 1648 DCDDFKHFQQL 1658

BLAST of Tan0001044 vs. NCBI nr
Match: XP_022986084.1 (probable ubiquitin-conjugating enzyme E2 23 isoform X1 [Cucurbita maxima])

HSP 1 Score: 2070.4 bits (5363), Expect = 0.0e+00
Identity = 1056/1157 (91.27%), Postives = 1094/1157 (94.55%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVS----------PSGNISTPTINNDEARRTNENTSD 60
            MEQ+ SVDKESLIGT NSY N DGVS           S N+   TINNDE RRTNENTSD
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTITSSENVHMTTINNDETRRTNENTSD 60

Query: 61   KPNIPHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFD 120
            KP++PHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDED+D+DGEDG D  GCDD D
Sbjct: 61   KPSVPHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDGEDGEDGGGCDDDD 120

Query: 121  GDGEKEGQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRG 180
            GDGEKEGQNKEN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRG
Sbjct: 121  GDGEKEGQNKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRG 180

Query: 181  FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG 240
            FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG
Sbjct: 181  FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG 240

Query: 241  PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSST 300
            PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRV+ASSST
Sbjct: 241  PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSST 300

Query: 301  VFKNSKWLSGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT 360
            VFKNSKWLSGLWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT
Sbjct: 301  VFKNSKWLSGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT 360

Query: 361  CFSHANWQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELS 420
            CFSHANWQLGDWCLLPSSFSSG TK+SSQT   DSVTN+LDS Q    CDSED VL+ELS
Sbjct: 361  CFSHANWQLGDWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELS 420

Query: 421  GTTESTDLDSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKK 480
            GTTESTDLDS+SACDGNYRNPECNILPESS+SRA KE +HETWPLHRKKIRKVV+RRDKK
Sbjct: 421  GTTESTDLDSLSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKK 480

Query: 481  ARKKEENFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKA 540
            ARKKEENFERALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKA
Sbjct: 481  ARKKEENFERALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKA 540

Query: 541  SDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDY 600
            SDNDDD++ESRRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDY
Sbjct: 541  SDNDDDISESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDY 600

Query: 601  DYCYGDVVVRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDD 660
            DYCYGDVVVRLSP+SDS E++S  NNTEEL  +SSTN +M+CTEK SGS KIEDPSCSDD
Sbjct: 601  DYCYGDVVVRLSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDD 660

Query: 661  CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA 720
            CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA
Sbjct: 661  CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA 720

Query: 721  ASWETVDDDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLA 780
            ASWETVDDDE DSV+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLA
Sbjct: 721  ASWETVDDDERDSVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLA 780

Query: 781  AGIFSRGPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGR 840
            AGIFSRGPRNP+ MD+DSHSESETQSLEIQA EEKDSGLQSSSLKSNSFDASDMNSDCGR
Sbjct: 781  AGIFSRGPRNPELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGR 840

Query: 841  GEDDDASESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQ 900
            GED+ ASE S+VLESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQ
Sbjct: 841  GEDNIASEPSEVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQ 900

Query: 901  TNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFH 960
            TN+GRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFH
Sbjct: 901  TNSGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFH 960

Query: 961  LPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVS 1020
            LPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVS
Sbjct: 961  LPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVS 1020

Query: 1021 LQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELV 1080
            LQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELV
Sbjct: 1021 LQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELV 1080

Query: 1081 KEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSS 1140
            KEHFRRRGY+ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SS
Sbjct: 1081 KEHFRRRGYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLMSS 1140

Query: 1141 LNEVGADCHDFKHFQQL 1148
            LNEVGADC DFKHFQQL
Sbjct: 1141 LNEVGADCDDFKHFQQL 1157

BLAST of Tan0001044 vs. NCBI nr
Match: KAG7010843.1 (putative ubiquitin-conjugating enzyme E2 23 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2068.9 bits (5359), Expect = 0.0e+00
Identity = 1056/1152 (91.67%), Postives = 1094/1152 (94.97%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+ SVDKESLIGT NSY N DGVS S N+   TINNDE RRTNENTSDKP+IPHIYRQ
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTTINNDETRRTNENTSDKPSIPHIYRQ 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITD----DEDDDDDGEDGVDDDGCDDFDGDGEKE 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITD    DED+D+DGEDG DDDGCDD DGDGEK 
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDEDEDGEDGEDDDGCDDDDGDGEKV 120

Query: 121  GQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDF 180
            GQNKEN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDF
Sbjct: 121  GQNKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRGFLHGDF 180

Query: 181  VAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRI 240
            VAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRI
Sbjct: 181  VAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRI 240

Query: 241  DDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSK 300
            DDVLDNVTVMFDDGSKCKVTKAEP+RLKPVSKNILEDANFPYYPGQRV+ASSSTVFKNSK
Sbjct: 241  DDVLDNVTVMFDDGSKCKVTKAEPMRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSK 300

Query: 301  WLSGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHAN 360
            WLSGLWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHAN
Sbjct: 301  WLSGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHAN 360

Query: 361  WQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTEST 420
            WQLGDWCLLPSSFSSG TK+SS+T   DSV N+LDS Q  G CDSED VL+ELSGTTEST
Sbjct: 361  WQLGDWCLLPSSFSSGLTKESSRTHRSDSVNNSLDSTQPEGVCDSEDVVLNELSGTTEST 420

Query: 421  DLDSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEE 480
            DLDS+SACDGNYRNPECNILPESS+SRA KE THETWPLHRKKIRKVV+RRDKKARKKEE
Sbjct: 421  DLDSLSACDGNYRNPECNILPESSNSRALKETTHETWPLHRKKIRKVVIRRDKKARKKEE 480

Query: 481  NFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDD 540
            NFERALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKASDNDDD
Sbjct: 481  NFERALLIINTKTKVDVAWQDGRTERGLDSTNLIPIDNPGDHEFVPEQYVVEKASDNDDD 540

Query: 541  VTESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGD 600
            ++ESRRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDYDYCYGD
Sbjct: 541  ISESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGD 600

Query: 601  VVVRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSD 660
            VVVRLSP+SDS E+MS  N+TEEL  +SSTN +M+CTEK SGS KIEDPSCSDDCIDFSD
Sbjct: 601  VVVRLSPVSDSIESMSLGNDTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSD 660

Query: 661  LSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV 720
            LSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV
Sbjct: 661  LSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV 720

Query: 721  DDDEIDSVENAK-EDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFS 780
            DDDE D V+NAK EDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLAAGIFS
Sbjct: 721  DDDERDCVDNAKEEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFS 780

Query: 781  RGPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDD 840
            RGPRNP+SMD+DSHSESETQSLEIQA EEKDSGLQSSSLKSNSFDASDMNSDCGRGED+ 
Sbjct: 781  RGPRNPESMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDNI 840

Query: 841  ASESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGR 900
            ASE S+V ESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTN+GR
Sbjct: 841  ASEPSEVSESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNSGR 900

Query: 901  KWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEY 960
            KWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEY
Sbjct: 901  KWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEY 960

Query: 961  PDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLV 1020
            PDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLV
Sbjct: 961  PDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLV 1020

Query: 1021 LNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFR 1080
            LNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFR
Sbjct: 1021 LNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR 1080

Query: 1081 RRGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVG 1140
            RRGY+ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVG
Sbjct: 1081 RRGYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLVSSLNEVG 1140

Query: 1141 ADCHDFKHFQQL 1148
            ADC DFKHFQQL
Sbjct: 1141 ADCDDFKHFQQL 1152

BLAST of Tan0001044 vs. NCBI nr
Match: XP_022943819.1 (probable ubiquitin-conjugating enzyme E2 23 [Cucurbita moschata])

HSP 1 Score: 2067.7 bits (5356), Expect = 0.0e+00
Identity = 1053/1149 (91.64%), Postives = 1092/1149 (95.04%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+ SVDKESLIGT NSYM+ DGVS S N+   TINNDE R+TNENTSDKP+IPHIYRQ
Sbjct: 1    MEQQGSVDKESLIGTGNSYMDRDGVSSSENVHMTTINNDETRKTNENTSDKPSIPHIYRQ 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITD--DEDDDDDGEDGVDDDGCDDFDGDGEKEGQ 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITD  DED+D+DG DG DDDGCDD DGDGEK GQ
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDEDGGDGEDDDGCDDDDGDGEKVGQ 120

Query: 121  NKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVA 180
            NKEN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVA
Sbjct: 121  NKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTPNVNDLTVIDRGFLHGDFVA 180

Query: 181  AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD 240
            AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD
Sbjct: 181  AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD 240

Query: 241  VLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWL 300
            VLDNVTVMFDDGSKCKVTKAEP+RLKPVSKNILEDANFPYYPGQRV+ASSSTVFKNSKWL
Sbjct: 241  VLDNVTVMFDDGSKCKVTKAEPMRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWL 300

Query: 301  SGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ 360
            SGLWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ
Sbjct: 301  SGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ 360

Query: 361  LGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTESTDL 420
            LGDWCLLPSSFSSG TK+SS+T   DSV N+LDS Q  G CDSED VL+ELSGTTESTDL
Sbjct: 361  LGDWCLLPSSFSSGLTKESSRTHRSDSVNNSLDSTQPEGVCDSEDVVLNELSGTTESTDL 420

Query: 421  DSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENF 480
            DS+SACDGNYR PECNILPESS+SRA KE THETWPLHRKKIRKVV+RRDKKARKKEENF
Sbjct: 421  DSLSACDGNYRYPECNILPESSNSRALKETTHETWPLHRKKIRKVVIRRDKKARKKEENF 480

Query: 481  ERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVT 540
            ERALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKASDNDDD++
Sbjct: 481  ERALLIINTKTKVDVAWQDGRTERGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDIS 540

Query: 541  ESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVV 600
            ESRRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDYDYCYGDVV
Sbjct: 541  ESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVV 600

Query: 601  VRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLS 660
            VRLSP+SDS E+MS  N+TEEL  +SSTN +M+CTEK SGS KIEDPSCSDDCIDFSDLS
Sbjct: 601  VRLSPVSDSIESMSLGNDTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLS 660

Query: 661  WVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD 720
            WVGNITGLKNGDIEVTWANG VSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD
Sbjct: 661  WVGNITGLKNGDIEVTWANGXVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD 720

Query: 721  DEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGP 780
            DE DSV+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLAAGIFSRGP
Sbjct: 721  DERDSVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSRGP 780

Query: 781  RNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASE 840
            RNP+SMD+DSHSESETQSLEIQA EEKDSGL SSSLKSNSFDASDMNSDCGRGED+ ASE
Sbjct: 781  RNPESMDVDSHSESETQSLEIQALEEKDSGLHSSSLKSNSFDASDMNSDCGRGEDNIASE 840

Query: 841  SSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWL 900
             S+V ESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWL
Sbjct: 841  PSEVSESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWL 900

Query: 901  KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960
            KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV
Sbjct: 901  KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960

Query: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1020
            PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVLNS
Sbjct: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVLNS 1020

Query: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRG 1080
            KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFRRRG
Sbjct: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRG 1080

Query: 1081 YYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1140
            Y+ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVGADC
Sbjct: 1081 YFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLVSSLNEVGADC 1140

Query: 1141 HDFKHFQQL 1148
             DFKHFQQL
Sbjct: 1141 DDFKHFQQL 1149

BLAST of Tan0001044 vs. ExPASy TrEMBL
Match: A0A6J1JD25 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483941 PE=4 SV=1)

HSP 1 Score: 2078.5 bits (5384), Expect = 0.0e+00
Identity = 1056/1147 (92.07%), Postives = 1094/1147 (95.38%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+ SVDKESLIGT NSY N DGVS S N+   TINNDE RRTNENTSDKP++PHIYRQ
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTTINNDETRRTNENTSDKPSVPHIYRQ 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNK 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITDDED+D+DGEDG D  GCDD DGDGEKEGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDGEDGEDGGGCDDDDGDGEKEGQNK 120

Query: 121  ENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180
            EN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  ENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRGFLHGDFVAAV 180

Query: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240
            SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL
Sbjct: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240

Query: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSG 300
            DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRV+ASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWLSG 300

Query: 301  LWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360
            LWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG
Sbjct: 301  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360

Query: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTESTDLDS 420
            DWCLLPSSFSSG TK+SSQT   DSVTN+LDS Q    CDSED VL+ELSGTTESTDLDS
Sbjct: 361  DWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELSGTTESTDLDS 420

Query: 421  ISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENFER 480
            +SACDGNYRNPECNILPESS+SRA KE +HETWPLHRKKIRKVV+RRDKKARKKEENFER
Sbjct: 421  LSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 481  ALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTES 540
            ALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKASDNDDD++ES
Sbjct: 481  ALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDISES 540

Query: 541  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVR 600
            RRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDYDYCYGDVVVR
Sbjct: 541  RRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVVVR 600

Query: 601  LSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWV 660
            LSP+SDS E++S  NNTEEL  +SSTN +M+CTEK SGS KIEDPSCSDDCIDFSDLSWV
Sbjct: 601  LSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLSWV 660

Query: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720
            GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE
Sbjct: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720

Query: 721  IDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGPRN 780
             DSV+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLAAGIFSRGPRN
Sbjct: 721  RDSVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSRGPRN 780

Query: 781  PDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASESS 840
            P+ MD+DSHSESETQSLEIQA EEKDSGLQSSSLKSNSFDASDMNSDCGRGED+ ASE S
Sbjct: 781  PELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDNIASEPS 840

Query: 841  KVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLKK 900
            +VLESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTN+GRKWLKK
Sbjct: 841  EVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNSGRKWLKK 900

Query: 901  IQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 960
            IQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP
Sbjct: 901  IQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPP 960

Query: 961  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKP 1020
            SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVLNSKP
Sbjct: 961  SAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVLNSKP 1020

Query: 1021 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYY 1080
            YFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFRRRGY+
Sbjct: 1021 YFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGYF 1080

Query: 1081 ILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCHD 1140
            ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVGADC D
Sbjct: 1081 ILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLMSSLNEVGADCDD 1140

Query: 1141 FKHFQQL 1148
            FKHFQQL
Sbjct: 1141 FKHFQQL 1147

BLAST of Tan0001044 vs. ExPASy TrEMBL
Match: A0A6J1JF34 (probable ubiquitin-conjugating enzyme E2 23 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483941 PE=4 SV=1)

HSP 1 Score: 2070.4 bits (5363), Expect = 0.0e+00
Identity = 1056/1157 (91.27%), Postives = 1094/1157 (94.55%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVS----------PSGNISTPTINNDEARRTNENTSD 60
            MEQ+ SVDKESLIGT NSY N DGVS           S N+   TINNDE RRTNENTSD
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTITSSENVHMTTINNDETRRTNENTSD 60

Query: 61   KPNIPHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFD 120
            KP++PHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDED+D+DGEDG D  GCDD D
Sbjct: 61   KPSVPHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDGEDGEDGGGCDDDD 120

Query: 121  GDGEKEGQNKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRG 180
            GDGEKEGQNKEN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRG
Sbjct: 121  GDGEKEGQNKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRG 180

Query: 181  FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG 240
            FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG
Sbjct: 181  FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG 240

Query: 241  PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSST 300
            PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRV+ASSST
Sbjct: 241  PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSST 300

Query: 301  VFKNSKWLSGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT 360
            VFKNSKWLSGLWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT
Sbjct: 301  VFKNSKWLSGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT 360

Query: 361  CFSHANWQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELS 420
            CFSHANWQLGDWCLLPSSFSSG TK+SSQT   DSVTN+LDS Q    CDSED VL+ELS
Sbjct: 361  CFSHANWQLGDWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELS 420

Query: 421  GTTESTDLDSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKK 480
            GTTESTDLDS+SACDGNYRNPECNILPESS+SRA KE +HETWPLHRKKIRKVV+RRDKK
Sbjct: 421  GTTESTDLDSLSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKK 480

Query: 481  ARKKEENFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKA 540
            ARKKEENFERALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKA
Sbjct: 481  ARKKEENFERALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKA 540

Query: 541  SDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDY 600
            SDNDDD++ESRRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDY
Sbjct: 541  SDNDDDISESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDY 600

Query: 601  DYCYGDVVVRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDD 660
            DYCYGDVVVRLSP+SDS E++S  NNTEEL  +SSTN +M+CTEK SGS KIEDPSCSDD
Sbjct: 601  DYCYGDVVVRLSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDD 660

Query: 661  CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA 720
            CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA
Sbjct: 661  CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA 720

Query: 721  ASWETVDDDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLA 780
            ASWETVDDDE DSV+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLA
Sbjct: 721  ASWETVDDDERDSVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLA 780

Query: 781  AGIFSRGPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGR 840
            AGIFSRGPRNP+ MD+DSHSESETQSLEIQA EEKDSGLQSSSLKSNSFDASDMNSDCGR
Sbjct: 781  AGIFSRGPRNPELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGR 840

Query: 841  GEDDDASESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQ 900
            GED+ ASE S+VLESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQ
Sbjct: 841  GEDNIASEPSEVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQ 900

Query: 901  TNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFH 960
            TN+GRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFH
Sbjct: 901  TNSGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFH 960

Query: 961  LPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVS 1020
            LPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVS
Sbjct: 961  LPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVS 1020

Query: 1021 LQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELV 1080
            LQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELV
Sbjct: 1021 LQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELV 1080

Query: 1081 KEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSS 1140
            KEHFRRRGY+ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SS
Sbjct: 1081 KEHFRRRGYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLMSS 1140

Query: 1141 LNEVGADCHDFKHFQQL 1148
            LNEVGADC DFKHFQQL
Sbjct: 1141 LNEVGADCDDFKHFQQL 1157

BLAST of Tan0001044 vs. ExPASy TrEMBL
Match: A0A6J1FVD7 (probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC111448445 PE=4 SV=1)

HSP 1 Score: 2067.7 bits (5356), Expect = 0.0e+00
Identity = 1053/1149 (91.64%), Postives = 1092/1149 (95.04%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+ SVDKESLIGT NSYM+ DGVS S N+   TINNDE R+TNENTSDKP+IPHIYRQ
Sbjct: 1    MEQQGSVDKESLIGTGNSYMDRDGVSSSENVHMTTINNDETRKTNENTSDKPSIPHIYRQ 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITD--DEDDDDDGEDGVDDDGCDDFDGDGEKEGQ 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITD  DED+D+DG DG DDDGCDD DGDGEK GQ
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDEDGGDGEDDDGCDDDDGDGEKVGQ 120

Query: 121  NKENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVA 180
            NKEN GDDSNAKNSN+DNYKSQPLP DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVA
Sbjct: 121  NKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTPNVNDLTVIDRGFLHGDFVA 180

Query: 181  AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD 240
            AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD
Sbjct: 181  AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD 240

Query: 241  VLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWL 300
            VLDNVTVMFDDGSKCKVTKAEP+RLKPVSKNILEDANFPYYPGQRV+ASSSTVFKNSKWL
Sbjct: 241  VLDNVTVMFDDGSKCKVTKAEPMRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWL 300

Query: 301  SGLWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ 360
            SGLWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ
Sbjct: 301  SGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ 360

Query: 361  LGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTESTDL 420
            LGDWCLLPSSFSSG TK+SS+T   DSV N+LDS Q  G CDSED VL+ELSGTTESTDL
Sbjct: 361  LGDWCLLPSSFSSGLTKESSRTHRSDSVNNSLDSTQPEGVCDSEDVVLNELSGTTESTDL 420

Query: 421  DSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENF 480
            DS+SACDGNYR PECNILPESS+SRA KE THETWPLHRKKIRKVV+RRDKKARKKEENF
Sbjct: 421  DSLSACDGNYRYPECNILPESSNSRALKETTHETWPLHRKKIRKVVIRRDKKARKKEENF 480

Query: 481  ERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVT 540
            ERALLIINTKTKVDVAWQDG+TE  LDST+LIPIDNPGDHEFVPEQYVVEKASDNDDD++
Sbjct: 481  ERALLIINTKTKVDVAWQDGRTERGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDIS 540

Query: 541  ESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVV 600
            ESRRVGVVKSVHAKERTACV WLKPVSRAEDPREFDI+EIVSVYELEGHPDYDYCYGDVV
Sbjct: 541  ESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVV 600

Query: 601  VRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLS 660
            VRLSP+SDS E+MS  N+TEEL  +SSTN +M+CTEK SGS KIEDPSCSDDCIDFSDLS
Sbjct: 601  VRLSPVSDSIESMSLGNDTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLS 660

Query: 661  WVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD 720
            WVGNITGLKNGDIEVTWANG VSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD
Sbjct: 661  WVGNITGLKNGDIEVTWANGXVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD 720

Query: 721  DEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGP 780
            DE DSV+NAKEDIELQD GVNSEEE SEQNNSGRNLALSVP AA+RF TRLAAGIFSRGP
Sbjct: 721  DERDSVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSRGP 780

Query: 781  RNPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASE 840
            RNP+SMD+DSHSESETQSLEIQA EEKDSGL SSSLKSNSFDASDMNSDCGRGED+ ASE
Sbjct: 781  RNPESMDVDSHSESETQSLEIQALEEKDSGLHSSSLKSNSFDASDMNSDCGRGEDNIASE 840

Query: 841  SSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWL 900
             S+V ESA+ASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWL
Sbjct: 841  PSEVSESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWL 900

Query: 901  KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960
            KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV
Sbjct: 901  KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960

Query: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1020
            PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVLNS
Sbjct: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVLNS 1020

Query: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRG 1080
            KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFRRRG
Sbjct: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRG 1080

Query: 1081 YYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1140
            Y+ILKACD+YMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEVGADC
Sbjct: 1081 YFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLVSSLNEVGADC 1140

Query: 1141 HDFKHFQQL 1148
             DFKHFQQL
Sbjct: 1141 DDFKHFQQL 1149

BLAST of Tan0001044 vs. ExPASy TrEMBL
Match: A0A6J1CWF5 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015425 PE=4 SV=1)

HSP 1 Score: 2059.6 bits (5335), Expect = 0.0e+00
Identity = 1053/1153 (91.33%), Postives = 1091/1153 (94.62%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            MEQ+DSVDKESLIGT +S++N DGVS SGNI  P INNDE +RT+ENTSDKPNIPH+YRQ
Sbjct: 1    MEQQDSVDKESLIGTSDSFINEDGVSSSGNIHMPIINNDETKRTSENTSDKPNIPHVYRQ 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNK 120
            DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGE GVDD+ C+++DG+GEKEGQN 
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEAGVDDEDCNNYDGEGEKEGQNM 120

Query: 121  ENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180
            EN GDDSN KN N+DNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  ENYGDDSNGKNGNRDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180

Query: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240
            SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL
Sbjct: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240

Query: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSG 300
            DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSG 300

Query: 301  LWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360
            LWKPNR+EGTVTKVTVGSVFIYWIASAGYGPDSSTTP EEQ+PKNLRLL+CFSHANWQLG
Sbjct: 301  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPTEEQSPKNLRLLSCFSHANWQLG 360

Query: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQT--VGACDSEDTVLDELSGTTESTDL 420
            DWCLLPSSFSSG +KDS Q E PDSVTNTLDS QT  VGACDSEDT+ DELSGTTESTDL
Sbjct: 361  DWCLLPSSFSSGLSKDSLQIEPPDSVTNTLDSVQTGQVGACDSEDTMPDELSGTTESTDL 420

Query: 421  DSISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENF 480
            DSISACDGNYRN ECN LPESSSSRAPKE THETWPLHRKKIRKVVVRRDKKARKKEENF
Sbjct: 421  DSISACDGNYRNLECNTLPESSSSRAPKETTHETWPLHRKKIRKVVVRRDKKARKKEENF 480

Query: 481  ERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVT 540
            ERALLIINTKTKVDVAWQDG+TE  LDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVT
Sbjct: 481  ERALLIINTKTKVDVAWQDGRTECALDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVT 540

Query: 541  ESRRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVV 600
            ESRRVGVVKSVHAKERTACVRWLKPV+RAEDPREFD EEIVSVYELEGHPDYDYCYGDVV
Sbjct: 541  ESRRVGVVKSVHAKERTACVRWLKPVARAEDPREFDTEEIVSVYELEGHPDYDYCYGDVV 600

Query: 601  VRLSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLS 660
            VRLSP+SD TEAMSS NN EEL  +SSTN VMSCTEKG  S  +EDPSC DDCIDFSDLS
Sbjct: 601  VRLSPVSDCTEAMSSGNNIEELKQQSSTNEVMSCTEKGPESKNVEDPSCGDDCIDFSDLS 660

Query: 661  WVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV-D 720
            WVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETV D
Sbjct: 661  WVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD 720

Query: 721  DDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRG 780
            DDE+DSVENAKEDIELQDTGVNSEEE+SEQN+SG NLALSVPLAALRFVTRLAAGIFSRG
Sbjct: 721  DDEMDSVENAKEDIELQDTGVNSEEEESEQNSSGSNLALSVPLAALRFVTRLAAGIFSRG 780

Query: 781  PRNPDSMDLDSHSESETQSLEIQASEEKDSGLQ---SSSLKSNSFDASDMNSDCGRGEDD 840
            PRN DSMDLDSH ESE QS   +ASEE DSGLQ   SSSLKSNSF  SDMNSDCGR EDD
Sbjct: 781  PRNSDSMDLDSHGESEIQSSGTKASEENDSGLQSRLSSSLKSNSFYTSDMNSDCGREEDD 840

Query: 841  DASESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNG 900
             ASE SKVLE+AEA S+LRTAESDGSAC+E GTCSFK FDIAKDPLDHYF+GTNGQ NNG
Sbjct: 841  VASEPSKVLETAEAFSDLRTAESDGSACYEGGTCSFKSFDIAKDPLDHYFIGTNGQINNG 900

Query: 901  RKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPE 960
            RKW KKIQQDWSILQNNLP+GIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPE
Sbjct: 901  RKWHKKIQQDWSILQNNLPNGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPE 960

Query: 961  YPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGL 1020
            YPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGL
Sbjct: 961  YPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGL 1020

Query: 1021 VLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHF 1080
            VLNSKPYFNEAGYDKQ+GTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFE+LVKEHF
Sbjct: 1021 VLNSKPYFNEAGYDKQVGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEDLVKEHF 1080

Query: 1081 RRRGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEV 1140
            RRRGY+ILKACD+YMKGHLIGSL+EDASIR ESDPNSTSVGFKLMLAKIVPKLFSSLNEV
Sbjct: 1081 RRRGYFILKACDAYMKGHLIGSLSEDASIRVESDPNSTSVGFKLMLAKIVPKLFSSLNEV 1140

Query: 1141 GADCHDFKHFQQL 1148
            GADCHDFKHFQQL
Sbjct: 1141 GADCHDFKHFQQL 1153

BLAST of Tan0001044 vs. ExPASy TrEMBL
Match: A0A6J1G6I7 (probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC111451318 PE=4 SV=1)

HSP 1 Score: 2054.6 bits (5322), Expect = 0.0e+00
Identity = 1045/1148 (91.03%), Postives = 1091/1148 (95.03%), Query Frame = 0

Query: 1    MEQRDSVDKESLIGTCNSYMNVDGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQ 60
            M Q+DSVD+ESL   C+SYMN DGVS SGNI  PTIN+DEARR NEN SDK NIPH+YR+
Sbjct: 1    MGQQDSVDEESLTDACDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 60

Query: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNK 120
            DIVKSKGSGMIGIVTEVAGDADSD+DITDDEDD DDGEDG+DDDGCDD+DGDGE EGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYDGDGENEGQNK 120

Query: 121  ENNGDDSNAKNSNKDNYKSQPLPVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180
            EN  DD+NAKNSN+D+YK+QPLP DEVRVLWMDESETTQNVNDLTV DRGFLHGDFVAAV
Sbjct: 121  ENYDDDTNAKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVTDRGFLHGDFVAAV 180

Query: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240
            S+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVRDF VGDYVVLGPWLGRIDDVL
Sbjct: 181  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVRASSSTVFKNSKWLSG 300
            DNVTVMFDDGSKCKVTKAEP RLKPVSKNILEDA+FPYYPGQRVRASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 300

Query: 301  LWKPNRMEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360
            LWKPNR++GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNLRLLTCFSH NWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTESTDLDS 420
            DWCLLPSSFSSGSTKD SQTEL DSVTNTLD AQTVGACDSEDTV+DELSG TESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 420

Query: 421  ISACDGNYRNPECNILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENFER 480
            ISAC+GNYR+PECNILPESSSSRA KENTHETWPLHRKKIRKVV+RRDKKARKKEENFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 481  ALLIINTKTKVDVAWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTES 540
            ALLIINT TKVDV WQDG+TEL LDST LIPI NPGDHEFVPEQYVVEKA+DNDDDVTES
Sbjct: 481  ALLIINTTTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKATDNDDDVTES 540

Query: 541  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVR 600
            RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEE+VSVYELEGHPDYDYCYGDVVVR
Sbjct: 541  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 600

Query: 601  LSPISDSTEAMSSENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWV 660
            LSP+SDS EA+SSENNTEELN +SSTNGVMSC EK SGS KIE PS SDDC DFSDLSWV
Sbjct: 601  LSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWV 660

Query: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720
            GNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDESI AGSE+SNGAASWETVDDD+
Sbjct: 661  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETVDDDD 720

Query: 721  ID-SVENAKEDIELQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGPR 780
             +  VENAKEDIELQDTGV+SEEE+SEQ+NSGR+LALSVPLAA RFVTRLA GIFSRGPR
Sbjct: 721  DEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPR 780

Query: 781  NPDSMDLDSHSESETQSLEIQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASES 840
            N +SMDLDSHSES+TQSLEIQ+ EE DSGLQSSSLKSNSF  SDMNSDCGRGEDDDASE 
Sbjct: 781  NRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASEP 840

Query: 841  SKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLK 900
            SK+L SAEASSNLRTA+SDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQT NGRKWLK
Sbjct: 841  SKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLK 900

Query: 901  KIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 960
            KIQQDW+ILQNNLPDGIYVRVYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPPEYPDVP
Sbjct: 901  KIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDVP 960

Query: 961  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1020
            PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK
Sbjct: 961  PSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSK 1020

Query: 1021 PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRGY 1080
            PYFNEAGYDKQIGTAEGEKNSLSYNENTFLL+CKTIMYLMRKPPKDFEELVKEHFRRRGY
Sbjct: 1021 PYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRGY 1080

Query: 1081 YILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCH 1140
            YILKACD+YMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 
Sbjct: 1081 YILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCR 1140

Query: 1141 DFKHFQQL 1148
            DFKHFQQL
Sbjct: 1141 DFKHFQQL 1147

BLAST of Tan0001044 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 1299.6 bits (3362), Expect = 0.0e+00
Identity = 710/1136 (62.50%), Postives = 854/1136 (75.18%), Query Frame = 0

Query: 23   DGVSPSGNISTPTINNDEARRTNENTSDKPNIPHIYRQDIVKSKGSGMIGIVTEVAGDAD 82
            D    S N+   +  +D     +   S+ PN   IYRQDIVK+K +G +G+V+EVAGD+D
Sbjct: 6    DDPGTSTNVGVDSSVDDSMASLSICDSEHPN---IYRQDIVKNKKTGSVGVVSEVAGDSD 65

Query: 83   SDSDITDDEDDDDDGEDGVDDDGCDDFDGDGEKEGQNKENNGDDSNAKNSNKDNYKSQPL 142
            SDSDI+D+E+DDDD ED  DDD  +D + +G+K  +    NGD     +    NYK   L
Sbjct: 66   SDSDISDEEEDDDDDEDNDDDD--EDVE-EGKKASEENVVNGDGEKKADG---NYKCGAL 125

Query: 143  PVDEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSDPTGQAGVVVDVNISVDLLVP 202
              D++RVLWMD +E  Q++ND+TVIDRGFLHGD+VA+ S+PTGQ GVVVDVNISVDLL P
Sbjct: 126  EGDQIRVLWMDNTEPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAP 185

Query: 203  DGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLR 262
            DGSI KDIS+K+LKRVRDF VGDYVV GPWLGRIDDVLDNVTV+FDDGS CKV + EPLR
Sbjct: 186  DGSIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLR 245

Query: 263  LKPVSKNIL-EDANFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRMEGTVTKVTVGSVFI 322
            LKP+ KN L EDANFPYYPGQRV+ASSS+VFKNS+WLSGLWKPNR+EGTVTKVT GS+F+
Sbjct: 246  LKPIPKNNLEEDANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFV 305

Query: 323  YWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLGDWCLLPSSFSSGST---KDSS 382
            YWIASAG+GPDSS +P EEQ P NL LL+CF+HANWQ+GDWCLLPS   S +    K  S
Sbjct: 306  YWIASAGFGPDSSVSPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVS 365

Query: 383  QTELPDSVTNTLDSAQTVGACDSEDTVLDELSGTTE-----STDLDSISACDGNYRNPEC 442
            +  L DS     D  Q +G  D ED V DE+SG  E     +  L  +++ D   RNP  
Sbjct: 366  KLRLYDS---QADRQQKIGR-DLED-VQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSV 425

Query: 443  NILPESSSSRAPKENTHETWPLHRKKIRKVVVRRDKKARKKEENFERALLIINTKTKVDV 502
            +           KE  HE WPLHRKKIRK+V+R+DKK +KKEE+FE+ALL++N++T+VDV
Sbjct: 426  S-----------KEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALLVVNSRTRVDV 485

Query: 503  AWQDGQTELELDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKE 562
            +WQDG  E   ++ +LIPI+ PGDHEFV EQYVVEK SD+ D+ TE RR GVVK+V+AK+
Sbjct: 486  SWQDGTIECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKD 545

Query: 563  RTACVRWLKPVSRAEDPREFDIEEIVSVYELEGHPDYDYCYGDVVVRLSPISDSTEAMSS 622
            RTA VRWL P+ RAE+PREF+ EEIVSVYELEGHPDYDYCYGDVVVRLSPI+ +  A S 
Sbjct: 546  RTASVRWLNPLRRAEEPREFEKEEIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSP 605

Query: 623  ENNTEELNGESSTNGVMSCTEKGSGSPKIEDPSCSDDCIDFSDLSWVGNITGLKNGDIEV 682
             N+ EE   +   NG    +E    +  + D   ++   D S LSWVGNITGLK+GDIEV
Sbjct: 606  GNSFEEATQQD--NGYQD-SESHQEAKILVDKEENEPSTDLSKLSWVGNITGLKDGDIEV 665

Query: 683  TWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENAKEDI-E 742
            TWA+G +STVGP A+YVVGRDDDDES+A  SE S+ AASWET++DD+  + E  +ED+  
Sbjct: 666  TWADGTISTVGPHAVYVVGRDDDDESVAGESETSD-AASWETLNDDDRGAPEIPEEDLGR 725

Query: 743  LQDTGVNSEEEDSEQNNSGRNLALSVPLAALRFVTRLAAGIFSRGPRNPDSMDLDSHSES 802
                  NS+ +   +N+SGRN AL++PLAA+ FVTRLA+GIFSR  ++ DS   D   E+
Sbjct: 726  SSSIEGNSDADIYAENDSGRNGALALPLAAIEFVTRLASGIFSRARKSVDSSSSDYTVEN 785

Query: 803  ETQSLE-IQASEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDDDASESSKVLESAEASSN 862
              +  E    S+E DS    S  K N  D      +C   E      ++K + S E S+ 
Sbjct: 786  VYKQAESTNPSDETDSLDDPSPSKVNVTD------NC---ESKGTQANAKNILSGETSTF 845

Query: 863  LRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLKKIQQDWSILQNN 922
            L   + D     E  +CSF+ FDI++DPLDH+FLG +GQ    R+W KK+ QDW ILQNN
Sbjct: 846  LE--DEDKPVPSEGDSCSFRRFDISQDPLDHHFLGVDGQKTKERQWFKKVDQDWKILQNN 905

Query: 923  LPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRI 982
            LPDGI+VR YEDRMDLLRAVIVGA+GTPYQDGLFFFDFHLP +YP VPPSAYYHSGGWR+
Sbjct: 906  LPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRL 965

Query: 983  NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQI 1042
            NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ+
Sbjct: 966  NPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQV 1025

Query: 1043 GTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKG 1102
            GTAEGEKNSL YNENTFLL+CKT+MYLMRKPPKDFEEL+K+HFR+RGYYILKACD+YMKG
Sbjct: 1026 GTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKG 1085

Query: 1103 HLIGSLTEDAS-IREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADCHDFKHFQQ 1147
            +LIGSLT+DAS I E S  NSTSVGFKLMLAKI PKLFS+L+EVGADC++F+H QQ
Sbjct: 1086 YLIGSLTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEFQHLQQ 1101

BLAST of Tan0001044 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 601.7 bits (1550), Expect = 1.3e-171
Identity = 372/991 (37.54%), Postives = 538/991 (54.29%), Query Frame = 0

Query: 156  ETTQNVNDLTVIDRGFLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDL 215
            ET   +++    +RGF++GD V + ++P+GQ+G V+++++ V+L    G IMK++ +K L
Sbjct: 41   ETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRL 100

Query: 216  KRVRDFTVGDYVVLGPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDAN 275
            +++R  ++ DYV+ GPW+GR+D +++ V+V  DDG+  +V      +L  +  N+LED+ 
Sbjct: 101  QKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQ 160

Query: 276  FPYYPGQRVRASSSTVFKNSKWLSGLWKPNRMEGTVTKVTVGSVFIYWIAS-AGYGPDSS 335
            + YYPGQRV+   +   +++ WL G W+  ++ GTV  V  G V++ W+AS    G  + 
Sbjct: 161  YSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNL 220

Query: 336  TTPAEEQTPKNLRLLTCFSHANWQLGDWCLLPSSFSSGSTKDSSQTELPDSVTNTLDSAQ 395
            T P   Q P++L LL C SHA+WQLGDWC+LP S    S  D ++ + P+     L+   
Sbjct: 221  TAPQALQNPESLTLLPCVSHASWQLGDWCILPGS----SHCDIAERQTPNVAAYNLN--- 280

Query: 396  TVGACDSEDTVLDELSGTTESTDLDSISACDGNYRNPECNILPESSSSRAPKENTHETWP 455
                                                 EC                H+T+ 
Sbjct: 281  -------------------------------------EC----------------HKTF- 340

Query: 456  LHRKKIRKVVVRRDKKARKKEENFERALLIINTKTKVDVAWQDGQTELELDSTSLIPIDN 515
                       ++      +    +   +I  TK KV V WQDG   L +DS  L+P+  
Sbjct: 341  -----------QKGFNRNMQNSGLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGA 400

Query: 516  PGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVSR-AEDPREF 575
               H+F PEQ+VVEK + N      S++ GVVK+V+AKE+T  V+W   V + A    + 
Sbjct: 401  VNAHDFWPEQFVVEKETCN------SKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDE 460

Query: 576  DIEEIVSVYELEGHPDYDYCYGDVVVRLSPIS--DSTEAMSSENNTEELNGESSTNGVMS 635
             +EEIVS YEL  HPD+ +C+ DVVV+L P    D           + L  ES  +G   
Sbjct: 461  VMEEIVSAYELLEHPDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKHLLTESDYSGAYF 520

Query: 636  CTEKGSGSPKIEDPSCSDDCIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVV 695
                                     LS +G +TG KNG ++V WANG  S V P  I+ +
Sbjct: 521  -------------------------LSSIGVVTGFKNGSVKVKWANGSTSKVAPCEIWKM 580

Query: 696  GRDDDDESIAAGSEVSNGAASWETVDDDEIDSVENAKEDIELQDTGVNSEEEDSEQNNSG 755
             R +   S    SE S    S +    DE  S       ++L   G +  E   E +   
Sbjct: 581  ERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVGESCNENIPECS--- 640

Query: 756  RNLALSVPLAALRFVTRLAAGIFSRGPRNPDSMDLDSHSESETQSLEIQASEEKDSGLQS 815
               +  +P AA+ F+T LA+ +F  G +   S+ + SHS           SE++      
Sbjct: 641  ---SFFLPKAAIGFITNLASSLF--GYQGSTSV-ISSHSRCND-------SEDQSDSEVL 700

Query: 816  SSLKSNSFDASDMNSDCGRGEDDDASESSKV-LESAEASSNLRTAESDGSACHEDGTCSF 875
                + S+D S+ NS    GE D  +    + +E    +  L +   + S         F
Sbjct: 701  VQETAESYDNSETNS----GEVDMTTTMVNIPIEGKGINKTLDSTLLENSR----NQVRF 760

Query: 876  KGFDIAKDPLDHYFLGTN---GQTNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDL 935
            + FD+  D  DH+FL ++    Q+   + W+KK+QQ+WS L+ NLP+ IYVRV E+RMDL
Sbjct: 761  RQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDL 820

Query: 936  LRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 995
            LRA +VGA GTPY DGLFFFD  LPP+YP  PP  +YHSGG R+NPNLYE G+VCLSLLN
Sbjct: 821  LRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLN 880

Query: 996  TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1055
            TW+G G EVW+  SSSILQ+L+S Q LVLN KPYFNEAGYDKQ+G AEGEKNS+SYNEN 
Sbjct: 881  TWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENA 899

Query: 1056 FLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREES 1115
            FL++CK+++ ++RKPPK FE LVK+HF  R  ++L AC +YM+G  +GS    A+++  S
Sbjct: 941  FLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS---SANLQGNS 899

Query: 1116 DPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1139
              NST  GFK+ML+K+ PKL  + +E+G DC
Sbjct: 1001 TTNST--GFKIMLSKLYPKLLEAFSEIGVDC 899

BLAST of Tan0001044 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 286.6 bits (732), Expect = 9.0e-77
Identity = 136/257 (52.92%), Postives = 177/257 (68.87%), Query Frame = 0

Query: 870  FKGFDIAKDPLDHYFLGTNGQTN--NGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDL 929
            FK FDI +D  DH++  + G T+  + + W K+IQ +W IL+ +LP+ I+VR YE RMDL
Sbjct: 305  FKRFDIVEDFSDHHY-ASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDL 364

Query: 930  LRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 989
            LRAVI+GA GTPY DGLFFFD   P  YP  PP  +YHSGG RINPNLY  GKVCLSLL 
Sbjct: 365  LRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLG 424

Query: 990  TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1049
            TW+G   E W P +S++LQVLVS+QGL+LN KPYFNE GY++  G+A GE  S +Y+ENT
Sbjct: 425  TWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENT 484

Query: 1050 FLLSCKTIMYLMRKPPKDFEELVKEHFRRRGYYILKACDSYMKGHLIGSLTEDASIREES 1109
            F+LS KT++Y MR+PPK FE+    HF    + +LKAC++Y  G   G L + A   EE+
Sbjct: 485  FILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEEN 544

Query: 1110 DPNSTSVGFKLMLAKIV 1125
                +S+ F+  +A  V
Sbjct: 545  SAGMSSLKFRTDVATFV 560

BLAST of Tan0001044 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 281.2 bits (718), Expect = 3.8e-75
Identity = 144/302 (47.68%), Postives = 190/302 (62.91%), Query Frame = 0

Query: 835  DASESSKVLESAEASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGT-NGQTNN 894
            DAS SS    +AE  S+++ + S+     E+    FK FD  +D  DH++         +
Sbjct: 210  DASASSSHPIAAEPISSVQDS-SNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQH 269

Query: 895  GRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPP 954
             + W+KK+Q DW IL+N+LP+ I VR  E RMDLLRAVI+GA GTPY DGLFFFD   P 
Sbjct: 270  SKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPD 329

Query: 955  EYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQG 1014
             YP VPP+ +YHSGG RINPNLY  GKVCLSLL TW G   E W P  S++LQ+LVS+Q 
Sbjct: 330  TYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQA 389

Query: 1015 LVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEH 1074
            L+LN KPYFNE GY +  GTA GE  S  Y+EN FLLS KT++Y +R+PP+ FEE V+ H
Sbjct: 390  LILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNH 449

Query: 1075 FRRRGYYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNE 1134
            +  R + I+KAC++Y  G  +GS+ +      E    S S  FK  +A  +  +     +
Sbjct: 450  YFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVK 509

Query: 1135 VG 1136
            +G
Sbjct: 510  LG 510

BLAST of Tan0001044 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 252.7 bits (644), Expect = 1.4e-66
Identity = 129/298 (43.29%), Postives = 179/298 (60.07%), Query Frame = 0

Query: 849  SSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQT----------NNGRKWL 908
            SSN+R  +       E+    FK FD  +D  DH++                    + W+
Sbjct: 7    SSNVRKLK-------EEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWV 66

Query: 909  KKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDV 968
            K IQ++W IL  NLP+ I+VR  E R+DLLRAVI+GA GTPY DGLFFFD   P  YP V
Sbjct: 67   KDIQKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSV 126

Query: 969  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1028
            PP  +YHSGG RINPNLY+ GKVCLSL++TWTG+  E W PK S++LQ+LVS+Q L+LN 
Sbjct: 127  PPKVHYHSGGLRINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNE 186

Query: 1029 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTIMYLMRKPPKDFEELVKEHFRRRG 1088
            KPY+NE GY+K +GT  GE  S  Y+EN F+ S KT+          FEE V+ HF  R 
Sbjct: 187  KPYYNEPGYEKSMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRS 246

Query: 1089 YYILKACDSYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGA 1137
            + I+KAC++Y  G  +GS+ +   +++++     S+ F++ +   +  +      +GA
Sbjct: 247  HDIVKACNAYKDGAPVGSI-DKGGVKKQTRQRG-SLKFRINVTSFMKTVVDEFVNLGA 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVX10.0e+0062.50Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q8VY101.8e-17037.54Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q6ZPJ37.2e-7926.19(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O OS=Mus musculus OX=10090 ... [more]
Q9C0C92.3e-7725.88(E3-independent) E2 ubiquitin-conjugating enzyme OS=Homo sapiens OX=9606 GN=UBE2... [more]
Q9LUQ51.3e-7552.92Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Match NameE-valueIdentityDescription
XP_022986085.10.0e+0092.07probable ubiquitin-conjugating enzyme E2 23 isoform X2 [Cucurbita maxima][more]
KAG6571012.10.0e+0091.75putative ubiquitin-conjugating enzyme E2 23, partial [Cucurbita argyrosperma sub... [more]
XP_022986084.10.0e+0091.27probable ubiquitin-conjugating enzyme E2 23 isoform X1 [Cucurbita maxima][more]
KAG7010843.10.0e+0091.67putative ubiquitin-conjugating enzyme E2 23 [Cucurbita argyrosperma subsp. argyr... [more]
XP_022943819.10.0e+0091.64probable ubiquitin-conjugating enzyme E2 23 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1JD250.0e+0092.07probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Cucurbita maxima OX=36... [more]
A0A6J1JF340.0e+0091.27probable ubiquitin-conjugating enzyme E2 23 isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1FVD70.0e+0091.64probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1CWF50.0e+0091.33probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Momordica charantia OX... [more]
A0A6J1G6I70.0e+0091.03probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G16920.10.0e+0062.50ubiquitin-conjugating enzyme 23 [more]
AT2G33770.11.3e-17137.54phosphate 2 [more]
AT3G15355.19.0e-7752.92ubiquitin-conjugating enzyme 25 [more]
AT1G53025.13.8e-7547.68Ubiquitin-conjugating enzyme family protein [more]
AT1G53023.11.4e-6643.29Ubiquitin-conjugating enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 899..1056
e-value: 4.1E-22
score: 89.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 609..645
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 731..752
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 609..634
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 774..862
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 793..824
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..42
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 842..857
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 826..841
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..141
NoneNo IPR availablePANTHERPTHR46116:SF21UBIQUITIN-CONJUGATING ENZYME E2 23-RELATEDcoord: 45..1146
NoneNo IPR availablePANTHERPTHR46116(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYMEcoord: 45..1146
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 865..1130
e-value: 4.3E-79
score: 267.8
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 880..1063
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 901..1045
e-value: 2.0E-22
score: 79.4
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 896..1056
score: 34.582172
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 899..1049
e-value: 1.1101E-32
score: 121.926

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001044.1Tan0001044.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061631 ubiquitin conjugating enzyme activity