Tan0000931 (gene) Snake gourd v1

Overview
NameTan0000931
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description5'-adenylylsulfate reductase 1
LocationLG05: 83012151 .. 83015091 (-)
RNA-Seq ExpressionTan0000931
SyntenyTan0000931
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCAGACAAGAAGAATTACAATTAACCGAAAGAGAGAGCAAGATTCTTTTGTCCTTCATGGCGCTTGCTTCTTCTTCTTCTGCAATTTCTGCTTCTAATTTGCAGCATTTTGCCGCTTCCTCTGAGGCTAAAGGTATTGTTCAGAAATGTAGTCTCTCGTTTAGGAAGCGAAGATGAAGACCTTTTTTAAGATTTATTTGTGTTTGAATGATGTTTCGATGAATTGCATGTTCGTTAGAATGTTAATTCTTAATTGTGGATATTGTTTTGATTTGCAGCTCCACGAATTGGTTCTGTTCAGCTGTTTAATCGGCCAAACGTTTTATCTGCTGGAGTCCGTTATTCATTAAGAGGATGCTCGTTGAAGCGTATTAATGGGGAATCCAAACGGAATGAATCGCTTGCTCCTCTCGCAGCGATTACCCTTGGTGCTGGTAAGTCTGATTTCTTAGTAGCCCCATCTCCTTGAATTAGAGTTCATTTCTGGGAATTTGTGTAATAAATGTTTGTGGTATGATTAATGATGATGTTTTGGGGCTGGATCTGCAGAGGTGGCAGAGAAAGCGGAGACGGTAGATTATGATGGGTTAGCTAAAGACCTTGAAAATGCTTCCCCTTTGGAAATAATGGACAAAGCCCTCGAGAAATTTGGCAACGACATTGCGATTGCATTCAGGTATGTTTTTATTTGATCATTTGATTAAATTGTCTTTAGCTTTTATTATCTAACATTATGTCTCAAATGTCAATGCTGTTGAATCTGTGATCTGTTAGGTGCCTCTTTAATCACATTTATGTTATTGTCATTCAAGGACAACATTTTTCATGCATCTTCTTAAATTTTCAAGTACCATGGAGATTCGTGTTACTGGAATTTATTTTTTGTTGACTAATTGATTCGGATTGTGTTAGTTTATTTATATTCTCTCTGCCGCCACCCGTTACCATTTCTTTCATTTTCCATAAACTTTTGGCTACAATCACCAGTAAATGATGGAGTATAATGTAACATAATGGAAATGTTGAAAAAAGGTGTGAACTAAGTGAAAATTGAATGAATTTTGTTAAAACTCAAAATTAATATACATAATTATAAACTAATAATATTAGATGTCGGATTGTTTTCTTTTAATATTGGAAAAGTATGTTTCTGATGTTCTTTCTGAACACTGAATTATAGATGCAAGGGTATCAATAATTTTCATCCTTGACAAGAATTTACTAGTAAAGCATTTGGTTTCTGATTCTTGGTCAGTTAAGTTTTAAAGAATCTCCTTATGAGCCTCTTACCTACAAAACATCTAAACGAAAACTATCCACTGCAGTGGCGCGGAAGATGTTGCATTGATTGAATATGCTCGTTTGACTGGTCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAAACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCACATCGAGTACATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGCTGTTTTCATTCTATGAGGATGGCCACCAGGAATGCTGCCGAGTAAGGAAGGTGAGGCCTTTGAGGAGGGCTCTAAAGGGATTCCGAGCATGGATCACAGGACAAAGAAAAGACCAATCTCCTGGAACTAGGTCTGAGATTCCTGTTGTCCAAGTCGATCCTGCTTTTGAAGGATTAGATGGTGGCATTGGGAGCCTGATTAAGTGGAACCCAGTAGCCAACGTTGATGGTAAAGACATATGGAACTTCTTGCGGACCATGAATGTGCCTGTGAACTCTTTACATTCTCAAGGATACGTCTCAATTGGCTGCGAGCCGTGCACAAGGCCTGTCCTCCCAGGGCAACACGAAAGAGAAGGAAGATGGTGGTGGGAGGATTCCAAGGCCAAGGAATGCGGTCTTCACAAAGGAAATCTTAAACAGGAAGGTGTCGCCCAGCTCAATGGCAATGGAAATGGAGCTCCCCCAGTTAACAACATCTTCATCTCCCAAAATTTGGTAAACCTGAACAGAACCGGAATAGAAAACTTGGCCAGACTGGAGAACCGGAAAGAACCTTGGCTTGTGGTTCTTTATGCACCTTGGTGCCAATTCTGCCAGGTAAGAATCATTTTGCAACAAAACATTTCAGTTCATAATCCTGATGTTAAGAACAAAAAGTAGAATACAATTGATTCCCAGTTTTGGTTTGGAATATATATCACTAATTGGCATATGTTTGGGCGAATTTGTACAGGCCATGGAAGGATCATATGTTGAATTGGCTGAGAAGTTGGGAGGGAGTGGAGTGAAAGTTGGGAAGTTCAGAGCGGACGGGGAGCAGAAAGAGTTCGCCAAACAAGAGCTGCAGCTAGGATCGTTTCCCACGATACTCTTTTTCCCCAAACACTCGTCTAAAGCAATCAAGTACCCATCTGAAAAGAGGGATGTTGACTCGTTGATGGCTTTTGTGAATGCCCTACGATGATATATAATACAAATATACACACGATATAGCAAAAGTCAAGTCCTAAATTCCAAAGACAGACAGTCCGACCGCCATTAATGAAGGAAAAATTCACACAGGTTCCAATCTTTCGTTAGATTCTGTGTACGTCTGAGGAAGGTTAATAACCAATGCGATGCAAGTTGAGATCAAAATCAAAGCTTGGGCATCTTTTTGGAGCTTGAAATCATTCGATTATGCTTTTGTCATTCGCGGGGCATGTACATGTATATATCAGTTTTTCCGTAGGCTCCCAGAAATAGGTTGTGGATTGTTTTATCAGATGATTTGTATGATGTCGCGTCTCTTTTGGTTCTACTAATGCAAGTTTGTGTAAAGTTGGGCTCGCACGAGGAGCCATGCTCATGTTTACAAAACCTCTCTTTATTTTTATTTCTTGAACTTGGGTGAAAATTAAACAGGGAGTGAGACTG

mRNA sequence

TCTCAGACAAGAAGAATTACAATTAACCGAAAGAGAGAGCAAGATTCTTTTGTCCTTCATGGCGCTTGCTTCTTCTTCTTCTGCAATTTCTGCTTCTAATTTGCAGCATTTTGCCGCTTCCTCTGAGGCTAAAGCTCCACGAATTGGTTCTGTTCAGCTGTTTAATCGGCCAAACGTTTTATCTGCTGGAGTCCGTTATTCATTAAGAGGATGCTCGTTGAAGCGTATTAATGGGGAATCCAAACGGAATGAATCGCTTGCTCCTCTCGCAGCGATTACCCTTGGTGCTGAGGTGGCAGAGAAAGCGGAGACGGTAGATTATGATGGGTTAGCTAAAGACCTTGAAAATGCTTCCCCTTTGGAAATAATGGACAAAGCCCTCGAGAAATTTGGCAACGACATTGCGATTGCATTCAGTGGCGCGGAAGATGTTGCATTGATTGAATATGCTCGTTTGACTGGTCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAAACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCACATCGAGTACATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGCTGTTTTCATTCTATGAGGATGGCCACCAGGAATGCTGCCGAGTAAGGAAGGTGAGGCCTTTGAGGAGGGCTCTAAAGGGATTCCGAGCATGGATCACAGGACAAAGAAAAGACCAATCTCCTGGAACTAGGTCTGAGATTCCTGTTGTCCAAGTCGATCCTGCTTTTGAAGGATTAGATGGTGGCATTGGGAGCCTGATTAAGTGGAACCCAGTAGCCAACGTTGATGGTAAAGACATATGGAACTTCTTGCGGACCATGAATGTGCCTGTGAACTCTTTACATTCTCAAGGATACGTCTCAATTGGCTGCGAGCCGTGCACAAGGCCTGTCCTCCCAGGGCAACACGAAAGAGAAGGAAGATGGTGGTGGGAGGATTCCAAGGCCAAGGAATGCGGTCTTCACAAAGGAAATCTTAAACAGGAAGGTGTCGCCCAGCTCAATGGCAATGGAAATGGAGCTCCCCCAGTTAACAACATCTTCATCTCCCAAAATTTGGTAAACCTGAACAGAACCGGAATAGAAAACTTGGCCAGACTGGAGAACCGGAAAGAACCTTGGCTTGTGGTTCTTTATGCACCTTGGTGCCAATTCTGCCAGGCCATGGAAGGATCATATGTTGAATTGGCTGAGAAGTTGGGAGGGAGTGGAGTGAAAGTTGGGAAGTTCAGAGCGGACGGGGAGCAGAAAGAGTTCGCCAAACAAGAGCTGCAGCTAGGATCGTTTCCCACGATACTCTTTTTCCCCAAACACTCGTCTAAAGCAATCAAGTACCCATCTGAAAAGAGGGATGTTGACTCGTTGATGGCTTTTGTGAATGCCCTACGATGATATATAATACAAATATACACACGATATAGCAAAAGTCAAGTCCTAAATTCCAAAGACAGACAGTCCGACCGCCATTAATGAAGGAAAAATTCACACAGGTTCCAATCTTTCGTTAGATTCTGTGTACGTCTGAGGAAGGTTAATAACCAATGCGATGCAAGTTGAGATCAAAATCAAAGCTTGGGCATCTTTTTGGAGCTTGAAATCATTCGATTATGCTTTTGTCATTCGCGGGGCATGTACATGTATATATCAGTTTTTCCGTAGGCTCCCAGAAATAGGTTGTGGATTGTTTTATCAGATGATTTGTATGATGTCGCGTCTCTTTTGGTTCTACTAATGCAAGTTTGTGTAAAGTTGGGCTCGCACGAGGAGCCATGCTCATGTTTACAAAACCTCTCTTTATTTTTATTTCTTGAACTTGGGTGAAAATTAAACAGGGAGTGAGACTG

Coding sequence (CDS)

ATGGCGCTTGCTTCTTCTTCTTCTGCAATTTCTGCTTCTAATTTGCAGCATTTTGCCGCTTCCTCTGAGGCTAAAGCTCCACGAATTGGTTCTGTTCAGCTGTTTAATCGGCCAAACGTTTTATCTGCTGGAGTCCGTTATTCATTAAGAGGATGCTCGTTGAAGCGTATTAATGGGGAATCCAAACGGAATGAATCGCTTGCTCCTCTCGCAGCGATTACCCTTGGTGCTGAGGTGGCAGAGAAAGCGGAGACGGTAGATTATGATGGGTTAGCTAAAGACCTTGAAAATGCTTCCCCTTTGGAAATAATGGACAAAGCCCTCGAGAAATTTGGCAACGACATTGCGATTGCATTCAGTGGCGCGGAAGATGTTGCATTGATTGAATATGCTCGTTTGACTGGTCGACCATTTAGGGTGTTTAGCCTAGACACAGGGAGGTTGAATTCAGAAACATACCAATTCTTTGATGCAGTGGAGAAACATTATGGCATCCACATCGAGTACATGTTCCCAGATGCTGTTGAAGTTCAGGGTTTAGTTCGGAACAAAGGGCTGTTTTCATTCTATGAGGATGGCCACCAGGAATGCTGCCGAGTAAGGAAGGTGAGGCCTTTGAGGAGGGCTCTAAAGGGATTCCGAGCATGGATCACAGGACAAAGAAAAGACCAATCTCCTGGAACTAGGTCTGAGATTCCTGTTGTCCAAGTCGATCCTGCTTTTGAAGGATTAGATGGTGGCATTGGGAGCCTGATTAAGTGGAACCCAGTAGCCAACGTTGATGGTAAAGACATATGGAACTTCTTGCGGACCATGAATGTGCCTGTGAACTCTTTACATTCTCAAGGATACGTCTCAATTGGCTGCGAGCCGTGCACAAGGCCTGTCCTCCCAGGGCAACACGAAAGAGAAGGAAGATGGTGGTGGGAGGATTCCAAGGCCAAGGAATGCGGTCTTCACAAAGGAAATCTTAAACAGGAAGGTGTCGCCCAGCTCAATGGCAATGGAAATGGAGCTCCCCCAGTTAACAACATCTTCATCTCCCAAAATTTGGTAAACCTGAACAGAACCGGAATAGAAAACTTGGCCAGACTGGAGAACCGGAAAGAACCTTGGCTTGTGGTTCTTTATGCACCTTGGTGCCAATTCTGCCAGGCCATGGAAGGATCATATGTTGAATTGGCTGAGAAGTTGGGAGGGAGTGGAGTGAAAGTTGGGAAGTTCAGAGCGGACGGGGAGCAGAAAGAGTTCGCCAAACAAGAGCTGCAGCTAGGATCGTTTCCCACGATACTCTTTTTCCCCAAACACTCGTCTAAAGCAATCAAGTACCCATCTGAAAAGAGGGATGTTGACTCGTTGATGGCTTTTGTGAATGCCCTACGATGA

Protein sequence

MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGESKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Homology
BLAST of Tan0000931 vs. ExPASy Swiss-Prot
Match: P92979 (5'-adenylylsulfate reductase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APR1 PE=1 SV=2)

HSP 1 Score: 685.6 bits (1768), Expect = 3.8e-196
Identity = 341/466 (73.18%), Postives = 392/466 (84.12%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYS-LRGCSLKRING 60
           M++  SSS+ S      F  S E K  +IGS++L +R +V    +  S  R  S+K +N 
Sbjct: 3   MSVNVSSSSSSGIINSRFGVSLEPKVSQIGSLRLLDRVHVAPVSLNLSGKRSSSVKPLNA 62

Query: 61  ESKRNESLAPLAAITLGAEVAEKAETV---DYDGLAKDLENASPLEIMDKALEKFGNDIA 120
           E K  +S+ PLAA T+ AE+AE+ E V   D++ LAK LENASPLEIMDKALEK+GNDIA
Sbjct: 63  EPKTKDSMIPLAA-TMVAEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGNDIA 122

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPD+VE
Sbjct: 123 IAFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPDSVE 182

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQ 240
           VQGLVR+KGLFSFYEDGHQECCRVRKVRPLRRALKG +AWITGQRKDQSPGTRSEIPVVQ
Sbjct: 183 VQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPVVQ 242

Query: 241 VDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDP FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GY+SIGCEPCT+ V
Sbjct: 243 VDPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCTKAV 302

Query: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQEG-VAQLNGNGNGAPPVNNIFISQNLVNLN 360
           LPGQHEREGRWWWED+KAKECGLHKGN+K+    A++NG    A  V +IF S+NLV L+
Sbjct: 303 LPGQHEREGRWWWEDAKAKECGLHKGNVKENSDDAKVNGESKSA--VADIFKSENLVTLS 362

Query: 361 RTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQK 420
           R GIENL +LENRKEPW+VVLYAPWC FCQAME SY ELA+KL GSG+KV KFRADG+QK
Sbjct: 363 RQGIENLMKLENRKEPWIVVLYAPWCPFCQAMEASYDELADKLAGSGIKVAKFRADGDQK 422

Query: 421 EFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           EFAKQELQLGSFPTIL FPK+SS+ IKYPSEKRDV+SL +F+N +R
Sbjct: 423 EFAKQELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465

BLAST of Tan0000931 vs. ExPASy Swiss-Prot
Match: P92980 (5'-adenylylsulfate reductase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APR3 PE=2 SV=2)

HSP 1 Score: 684.5 bits (1765), Expect = 8.4e-196
Identity = 338/461 (73.32%), Postives = 386/461 (83.73%), Query Frame = 0

Query: 4   ASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGESKR 63
           +SSSSAIS+S+      SS+ K  +IGS++L NR NV +A +  S +  S+K +N +S  
Sbjct: 9   SSSSSAISSSSF----PSSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKALNVQSIT 68

Query: 64  NESLAPLAAITLGAEVAEKAETV---DYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 123
            ES+       + +EV EK + V   D++ LAK LENASPLEIMDKALEKFGNDIAIAFS
Sbjct: 69  KESI-------VASEVTEKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAIAFS 128

Query: 124 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 183
           GAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEVQ L
Sbjct: 129 GAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEVQAL 188

Query: 184 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 243
           VRNKGLFSFYEDGHQECCR+RKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 189 VRNKGLFSFYEDGHQECCRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 248

Query: 244 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 303
           FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VLPGQ
Sbjct: 249 FEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVLPGQ 308

Query: 304 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 363
           HEREGRWWWED+KAKECGLHKGN+K+        N NG   V +IF S+N+VNL+R GIE
Sbjct: 309 HEREGRWWWEDAKAKECGLHKGNIKENTNGNATANVNGTASVADIFNSENVVNLSRQGIE 368

Query: 364 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 423
           NL +LENRKE W+VVLYAPWC FCQAME S+ ELA+KLGGSGVKV KFRADG+QK+FAK+
Sbjct: 369 NLMKLENRKEAWIVVLYAPWCPFCQAMEASFDELADKLGGSGVKVAKFRADGDQKDFAKK 428

Query: 424 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTIL FPK+SS+ IKYPSEKRDVDSL +F+N +R
Sbjct: 429 ELQLGSFPTILVFPKNSSRPIKYPSEKRDVDSLTSFLNLVR 458

BLAST of Tan0000931 vs. ExPASy Swiss-Prot
Match: P92981 (5'-adenylylsulfate reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APR2 PE=1 SV=2)

HSP 1 Score: 675.6 bits (1742), Expect = 3.9e-193
Identity = 339/464 (73.06%), Postives = 386/464 (83.19%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           +A+ SSS+AIS S+     ASSE+KA +I S++L +R ++       S R  S+K +N E
Sbjct: 3   LAVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHL-------SQRRYSMKPLNAE 62

Query: 61  S-KRNESLAPLAAITLGAEVAEK-AETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIA 120
           S  R+ES    A+  +  EV EK  E  D++ LAK LE+ASPLEIMDKALE+FG+ IAIA
Sbjct: 63  SHSRSESWVTRASTLIAPEVEEKGGEVEDFEQLAKKLEDASPLEIMDKALERFGDQIAIA 122

Query: 121 FSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQ 180
           FSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAVEVQ
Sbjct: 123 FSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAVEVQ 182

Query: 181 GLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVD 240
            LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKG +AWITGQRKDQSPGTRSEIP+VQVD
Sbjct: 183 ALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIVQVD 242

Query: 241 PAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP 300
           P FEGLDGG+GSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRPVLP
Sbjct: 243 PVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPVLP 302

Query: 301 GQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAP-PVNNIFISQNLVNLNRT 360
           GQHEREGRWWWED+KAKECGLHKGN+K+E     +G  +  P  V  IF S N+V L++ 
Sbjct: 303 GQHEREGRWWWEDAKAKECGLHKGNIKEE-----DGAADSKPAAVQEIFESNNVVALSKG 362

Query: 361 GIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEF 420
           G+ENL +LENRKE WLVVLYAPWC FCQAME SY+ELAEKL G GVKV KFRADGEQKEF
Sbjct: 363 GVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADGEQKEF 422

Query: 421 AKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           AKQELQLGSFPTIL FPK + +AIKYPSE RDVDSLM+FVN LR
Sbjct: 423 AKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 454

BLAST of Tan0000931 vs. ExPASy Swiss-Prot
Match: Q6Z4A7 (Probable 5'-adenylylsulfate reductase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=APR1 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 2.6e-181
Identity = 324/479 (67.64%), Postives = 372/479 (77.66%), Query Frame = 0

Query: 3   LASSSSAISASNLQHFAASSEAKAPRIGSV--QLFNRPNVLSAGVRYS-------LRGCS 62
           +AS++++IS+    H  A  + KA RIG+V  Q+   P   +A  R         LR   
Sbjct: 1   MASATASISS----HSVALRDLKAARIGAVKQQVAAAPAAGTAAARAQRARAVRPLRAAE 60

Query: 63  LKR--INGESKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKF 122
             R  ++  +    + AP+A     A V   A  VDY+ LA++L+ ASPLEIMD+AL  F
Sbjct: 61  PARQPVSASAAAAPAAAPVAEDAAAAAVDAPAPAVDYEALAQELQGASPLEIMDRALAMF 120

Query: 123 GNDIAIAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMF 182
           G+DIAIAFSGAEDVALIEYA+LTGRPFRVFSLDTGRLN ETYQ FD VEKHYGI IEYMF
Sbjct: 121 GSDIAIAFSGAEDVALIEYAKLTGRPFRVFSLDTGRLNPETYQLFDKVEKHYGIRIEYMF 180

Query: 183 PDAVEVQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSE 242
           PDA EVQ LVR KGLFSFYEDGHQECCR RKVRPLRRAL+G RAWITGQRKDQSPGTR+ 
Sbjct: 181 PDAGEVQALVRAKGLFSFYEDGHQECCRARKVRPLRRALRGLRAWITGQRKDQSPGTRAA 240

Query: 243 IPVVQVDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEP 302
           IPVVQVDP+FEGL GG GSL+KWNPVANVDGKD+W FLR M+VPVN+LH+QGYVSIGCEP
Sbjct: 241 IPVVQVDPSFEGLAGGAGSLVKWNPVANVDGKDVWTFLRAMDVPVNALHAQGYVSIGCEP 300

Query: 303 CTRPVLPGQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQL-------NGNGNGAPPVNN 362
           CTRPVLPGQHEREGRWWWED+KAKECGLHKGN+  +G A           NGNG+    +
Sbjct: 301 CTRPVLPGQHEREGRWWWEDAKAKECGLHKGNIDDQGGAAAAAAHKAGGANGNGSAGAPD 360

Query: 363 IFISQNLVNLNRTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGS--G 422
           IF S  +V+L R G+ENL RLE+R EPWLVVLYAPWC FCQAME SY+ELAE+LGG+  G
Sbjct: 361 IFESSGVVSLTRAGVENLLRLESRAEPWLVVLYAPWCPFCQAMEASYLELAERLGGAGGG 420

Query: 423 VKVGKFRADGEQKEFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           VKVGKFRADGEQK FA+QELQL SFPTIL FP  +++ IKYPSEKRDVDSL+AFVN+LR
Sbjct: 421 VKVGKFRADGEQKAFAQQELQLQSFPTILLFPSRTARPIKYPSEKRDVDSLLAFVNSLR 475

BLAST of Tan0000931 vs. ExPASy Swiss-Prot
Match: B7VBC3 (Adenosine 5'-phosphosulfate reductase OS=Pseudomonas aeruginosa (strain LESB58) OX=557722 GN=cysH PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 6.4e-79
Identity = 131/242 (54.13%), Postives = 177/242 (73.14%), Query Frame = 0

Query: 83  AETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFSGAEDVALIEYARLTGRPFRVFS 142
           ++  D   LA  L + SP +I+  A E FG+++ I+FSGAEDV L++ A    R  +VFS
Sbjct: 25  SQPFDLPALASSLADKSPQDILKAAFEHFGDELWISFSGAEDVVLVDMAWKLNRNVKVFS 84

Query: 143 LDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGLVRNKGLFSFYEDGHQECCRVRK 202
           LDTGRL+ ETY+F D V +HYGI I+ + PD   ++ LV+ KGLFSFY DGH ECC +RK
Sbjct: 85  LDTGRLHPETYRFIDQVREHYGIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRK 144

Query: 203 VRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPAFEGLDGGIGSLIKWNPVANVDG 262
           + PL+R L G RAW TGQR+DQSPGTRS++ V+++D AF   +     L K+NP++++  
Sbjct: 145 IEPLKRKLAGVRAWATGQRRDQSPGTRSQVAVLEIDGAFSTPE---KPLYKFNPLSSMTS 204

Query: 263 KDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDSKAKECGLHKG 322
           +++W ++R + +P NSLH +GY+SIGCEPCTRPVLP QHEREGRWWWE++  KECGLH G
Sbjct: 205 EEVWGYIRMLELPYNSLHERGYISIGCEPCTRPVLPNQHEREGRWWWEEATHKECGLHAG 263

Query: 323 NL 325
           NL
Sbjct: 265 NL 263

BLAST of Tan0000931 vs. NCBI nr
Match: XP_038895661.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 845.9 bits (2184), Expect = 1.7e-241
Identity = 420/464 (90.52%), Postives = 435/464 (93.75%), Query Frame = 0

Query: 1   MALA-SSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRING 60
           MAL+ SS S+ISASN QHF ASSEAK PRIGSVQL NRP+VLS+GV YS RGCS+K +N 
Sbjct: 1   MALSVSSHSSISASNFQHFPASSEAKVPRIGSVQLVNRPHVLSSGVNYSQRGCSMKPMNA 60

Query: 61  ESKRNESLAPL--AAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAI 120
           +S RN SL  L  AA TL AEVAE+AE V+YDGLAK LENASPLEIMD+ALEKFG+DIAI
Sbjct: 61  QSNRNVSLVSLAVAANTLTAEVAERAEIVNYDGLAKHLENASPLEIMDQALEKFGDDIAI 120

Query: 121 AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV 180
           AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV
Sbjct: 121 AFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEV 180

Query: 181 QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQV 240
           QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQV
Sbjct: 181 QGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQV 240

Query: 241 DPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL 300
           DP FEGLDGGIGSLIKWNPVANVDGKD+WNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL
Sbjct: 241 DPVFEGLDGGIGSLIKWNPVANVDGKDVWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVL 300

Query: 301 PGQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRT 360
           PGQHEREGRWWWEDSKAKECGLHKGNLKQ  V QLNGN NG PPVN+IFISQNLVNLNRT
Sbjct: 301 PGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNENGTPPVNDIFISQNLVNLNRT 360

Query: 361 GIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEF 420
           GIENLARL+NRKEPWLVVLYAPWCQFCQ MEGSYVELAEKLGGSGVKVGKFRADGEQK+F
Sbjct: 361 GIENLARLDNRKEPWLVVLYAPWCQFCQGMEGSYVELAEKLGGSGVKVGKFRADGEQKQF 420

Query: 421 AKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           AKQELQLGSFPTIL FPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 421 AKQELQLGSFPTILLFPKHSSKAIKYPSEKRDVDSLMAFVNALR 464

BLAST of Tan0000931 vs. NCBI nr
Match: XP_022923952.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita moschata] >KAG7019694.1 5'-adenylylsulfate reductase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 836.3 bits (2159), Expect = 1.3e-238
Identity = 411/461 (89.15%), Postives = 424/461 (91.97%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           MALA+S    S SNL HFA SS+AKAPRIGSVQL N P+  S+GV Y  RGC +K IN +
Sbjct: 1   MALAAS----SVSNLLHFAVSSDAKAPRIGSVQLLNWPHASSSGVNYLRRGCWMKPINSQ 60

Query: 61  SKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 120
           S+RN    PLA  T+ +EV EKAETVDYDGLAKDLENASPLEIMD+ALE+FGNDIAIAFS
Sbjct: 61  SQRNNLFVPLAVNTIASEVVEKAETVDYDGLAKDLENASPLEIMDQALEEFGNDIAIAFS 120

Query: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180
           GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL
Sbjct: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180

Query: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 240
           VRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 240

Query: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 300
           FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVN LHSQGYVSIGCEPCTRPVLP Q
Sbjct: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNPLHSQGYVSIGCEPCTRPVLPWQ 300

Query: 301 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 360
           HEREGRWWWEDSKAKECGLHKGNLKQ  + QLNGNGNG PPVN+IFISQNLVNLNRTGIE
Sbjct: 301 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPPVNDIFISQNLVNLNRTGIE 360

Query: 361 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 420
           NLARL NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKL GSGVKVGKFRADGEQKEFAK 
Sbjct: 361 NLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKS 420

Query: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 457

BLAST of Tan0000931 vs. NCBI nr
Match: KAG6584094.1 (5'-adenylylsulfate reductase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 835.5 bits (2157), Expect = 2.2e-238
Identity = 411/461 (89.15%), Postives = 423/461 (91.76%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           MALA+S    S SNL HFA SS+AKAPRIGSVQL N P+  S+GV Y  RGC +K IN  
Sbjct: 1   MALAAS----SVSNLLHFAVSSDAKAPRIGSVQLLNWPHASSSGVNYLRRGCWMKPINSR 60

Query: 61  SKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 120
           S+RN    PLA  T+ +EV EKAETVDYDGLAKDLENASPLEIMD+ALE+FGNDIAIAFS
Sbjct: 61  SQRNNLFVPLAVNTIASEVVEKAETVDYDGLAKDLENASPLEIMDQALEEFGNDIAIAFS 120

Query: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180
           GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL
Sbjct: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180

Query: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 240
           VRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 240

Query: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 300
           FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVN LHSQGYVSIGCEPCTRPVLP Q
Sbjct: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNPLHSQGYVSIGCEPCTRPVLPWQ 300

Query: 301 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 360
           HEREGRWWWEDSKAKECGLHKGNLKQ  + QLNGNGNG PPVN+IFISQNLVNLNRTGIE
Sbjct: 301 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPPVNDIFISQNLVNLNRTGIE 360

Query: 361 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 420
           NLARL NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKL GSGVKVGKFRADGEQKEFAK 
Sbjct: 361 NLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKS 420

Query: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 457

BLAST of Tan0000931 vs. NCBI nr
Match: XP_023520098.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 834.3 bits (2154), Expect = 5.0e-238
Identity = 412/461 (89.37%), Postives = 424/461 (91.97%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           MALA+S    S SNL HFA SS+AKAPRIGSVQL N P+VLS+GV Y  RGC +K IN +
Sbjct: 1   MALAAS----SVSNLLHFAVSSDAKAPRIGSVQLLNWPHVLSSGVNYLRRGCWMKPINSQ 60

Query: 61  SKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 120
           S+RN    PLA  TL +EV EKAETVDYDGLAK LENASPLEIMD+ALE+FGNDIAIAFS
Sbjct: 61  SQRNNLFVPLAVNTLASEVVEKAETVDYDGLAKHLENASPLEIMDQALEEFGNDIAIAFS 120

Query: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180
           GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL
Sbjct: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180

Query: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 240
           VRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 240

Query: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 300
           FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP Q
Sbjct: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQ 300

Query: 301 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 360
           HEREGRWWWEDSKAKECGLHKGNLKQ  + QLNGNGNG P VN+IFISQNLVNLNRTGIE
Sbjct: 301 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIE 360

Query: 361 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 420
           NLARL NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKL GSGVKVGKFRADGEQKEFAK 
Sbjct: 361 NLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKA 420

Query: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLM FVNALR
Sbjct: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMGFVNALR 457

BLAST of Tan0000931 vs. NCBI nr
Match: XP_023001259.1 (5'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 831.6 bits (2147), Expect = 3.2e-237
Identity = 409/461 (88.72%), Postives = 425/461 (92.19%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           MALA+S    S SNL HFA SS+AKAPRIGSVQ  N P+VLS+GV Y  RGC +K IN +
Sbjct: 1   MALAAS----SVSNLLHFAVSSDAKAPRIGSVQPLNWPHVLSSGVNYLRRGCRVKPINSQ 60

Query: 61  SKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 120
           S+RN+   PLA  TL +EV EKAETVDYDGLAKD E+ASPLEIMD+ALEKFGNDIAIAFS
Sbjct: 61  SQRNDLFVPLAVNTLASEVVEKAETVDYDGLAKDFEDASPLEIMDQALEKFGNDIAIAFS 120

Query: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180
           GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL
Sbjct: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180

Query: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 240
           VRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 240

Query: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 300
           FEGLDGGIGSLIKWNP+ANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP Q
Sbjct: 241 FEGLDGGIGSLIKWNPLANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQ 300

Query: 301 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 360
           HEREGRWWWEDSKAKECGLHKGNLKQ  + QLNGNGNG P VN+IFISQNLVNLNRTGIE
Sbjct: 301 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIE 360

Query: 361 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 420
           NL+RL NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKL GSGVKVGKFRADGEQKEFAK 
Sbjct: 361 NLSRLVNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKA 420

Query: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSL+AFVNALR
Sbjct: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLLAFVNALR 457

BLAST of Tan0000931 vs. ExPASy TrEMBL
Match: A0A6J1E7I7 (5'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431516 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 6.3e-239
Identity = 411/461 (89.15%), Postives = 424/461 (91.97%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           MALA+S    S SNL HFA SS+AKAPRIGSVQL N P+  S+GV Y  RGC +K IN +
Sbjct: 1   MALAAS----SVSNLLHFAVSSDAKAPRIGSVQLLNWPHASSSGVNYLRRGCWMKPINSQ 60

Query: 61  SKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 120
           S+RN    PLA  T+ +EV EKAETVDYDGLAKDLENASPLEIMD+ALE+FGNDIAIAFS
Sbjct: 61  SQRNNLFVPLAVNTIASEVVEKAETVDYDGLAKDLENASPLEIMDQALEEFGNDIAIAFS 120

Query: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180
           GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL
Sbjct: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180

Query: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 240
           VRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 240

Query: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 300
           FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVN LHSQGYVSIGCEPCTRPVLP Q
Sbjct: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNPLHSQGYVSIGCEPCTRPVLPWQ 300

Query: 301 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 360
           HEREGRWWWEDSKAKECGLHKGNLKQ  + QLNGNGNG PPVN+IFISQNLVNLNRTGIE
Sbjct: 301 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPPVNDIFISQNLVNLNRTGIE 360

Query: 361 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 420
           NLARL NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKL GSGVKVGKFRADGEQKEFAK 
Sbjct: 361 NLARLGNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKS 420

Query: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR
Sbjct: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 457

BLAST of Tan0000931 vs. ExPASy TrEMBL
Match: A0A6J1KM85 (5'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495445 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.6e-237
Identity = 409/461 (88.72%), Postives = 425/461 (92.19%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           MALA+S    S SNL HFA SS+AKAPRIGSVQ  N P+VLS+GV Y  RGC +K IN +
Sbjct: 1   MALAAS----SVSNLLHFAVSSDAKAPRIGSVQPLNWPHVLSSGVNYLRRGCRVKPINSQ 60

Query: 61  SKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 120
           S+RN+   PLA  TL +EV EKAETVDYDGLAKD E+ASPLEIMD+ALEKFGNDIAIAFS
Sbjct: 61  SQRNDLFVPLAVNTLASEVVEKAETVDYDGLAKDFEDASPLEIMDQALEKFGNDIAIAFS 120

Query: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180
           GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL
Sbjct: 121 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 180

Query: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 240
           VRNKGLFSFYEDGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 181 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 240

Query: 241 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 300
           FEGLDGGIGSLIKWNP+ANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP Q
Sbjct: 241 FEGLDGGIGSLIKWNPLANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPWQ 300

Query: 301 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 360
           HEREGRWWWEDSKAKECGLHKGNLKQ  + QLNGNGNG P VN+IFISQNLVNLNRTGIE
Sbjct: 301 HEREGRWWWEDSKAKECGLHKGNLKQGDIPQLNGNGNGTPLVNDIFISQNLVNLNRTGIE 360

Query: 361 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 420
           NL+RL NRKEPWLVVLYAPWCQFCQAMEGSYVEL EKL GSGVKVGKFRADGEQKEFAK 
Sbjct: 361 NLSRLVNRKEPWLVVLYAPWCQFCQAMEGSYVELGEKLAGSGVKVGKFRADGEQKEFAKA 420

Query: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSL+AFVNALR
Sbjct: 421 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLLAFVNALR 457

BLAST of Tan0000931 vs. ExPASy TrEMBL
Match: A0A5A7UP26 (5'-adenylylsulfate reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005680 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.3e-236
Identity = 411/465 (88.39%), Postives = 435/465 (93.55%), Query Frame = 0

Query: 1   MALASSSSAI-SASNLQHFAA-SSEAKAPRIGSVQLFNRPNVLSAGVRYS-LRGCS-LKR 60
           MAL+ SSS+I S+SN  HFAA SSE K PRIGSVQL +RP+VLS+GV YS + GCS +K 
Sbjct: 1   MALSVSSSSISSSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVVKP 60

Query: 61  INGESKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIA 120
           +N +SKR++SL P  A TL AEVAEK E VDYDGLA+DLENASPLEIMD+ALEKFG+DIA
Sbjct: 61  MNAQSKRDDSLVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDDIA 120

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE
Sbjct: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQ 240
           VQGLVRNKGLFSFY+DGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRS++PVVQ
Sbjct: 181 VQGLVRNKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPVVQ 240

Query: 241 VDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDP FEGLDGGIGSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV
Sbjct: 241 VDPVFEGLDGGIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300

Query: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNR 360
           LPGQHEREGRWWWEDSKAKECGLHKGNLKQ  V QLNGNGN  PPVN+IFISQ+LVNLNR
Sbjct: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNGNEIPPVNDIFISQDLVNLNR 360

Query: 361 TGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKE 420
           TG+ENLARL+NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKL GSGVKVGKFRADGEQK+
Sbjct: 361 TGMENLARLDNRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADGEQKQ 420

Query: 421 FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDV+SLMAFVNALR
Sbjct: 421 FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Tan0000931 vs. ExPASy TrEMBL
Match: E5GC59 (Adenylyl-sulfate reductase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.3e-236
Identity = 411/465 (88.39%), Postives = 435/465 (93.55%), Query Frame = 0

Query: 1   MALASSSSAI-SASNLQHFAA-SSEAKAPRIGSVQLFNRPNVLSAGVRYS-LRGCS-LKR 60
           MAL+ SSS+I S+SN  HFAA SSE K PRIGSVQL +RP+VLS+GV YS + GCS +K 
Sbjct: 1   MALSVSSSSISSSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVVKP 60

Query: 61  INGESKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIA 120
           +N +SKR++SL P  A TL AEVAEK E VDYDGLA+DLENASPLEIMD+ALEKFG+DIA
Sbjct: 61  MNAQSKRDDSLVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDDIA 120

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE
Sbjct: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQ 240
           VQGLVRNKGLFSFY+DGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRS++PVVQ
Sbjct: 181 VQGLVRNKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPVVQ 240

Query: 241 VDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDP FEGLDGGIGSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV
Sbjct: 241 VDPVFEGLDGGIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300

Query: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNR 360
           LPGQHEREGRWWWEDSKAKECGLHKGNLKQ  V QLNGNGN  PPVN+IFISQ+LVNLNR
Sbjct: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNGNEIPPVNDIFISQDLVNLNR 360

Query: 361 TGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKE 420
           TG+ENLARL+NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKL GSGVKVGKFRADGEQK+
Sbjct: 361 TGMENLARLDNRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADGEQKQ 420

Query: 421 FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDV+SLMAFVNALR
Sbjct: 421 FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Tan0000931 vs. ExPASy TrEMBL
Match: A0A1S3B2C8 (5'-adenylylsulfate reductase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485224 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.3e-236
Identity = 411/465 (88.39%), Postives = 435/465 (93.55%), Query Frame = 0

Query: 1   MALASSSSAI-SASNLQHFAA-SSEAKAPRIGSVQLFNRPNVLSAGVRYS-LRGCS-LKR 60
           MAL+ SSS+I S+SN  HFAA SSE K PRIGSVQL +RP+VLS+GV YS + GCS +K 
Sbjct: 1   MALSVSSSSISSSSNFHHFAASSSEPKVPRIGSVQLLHRPHVLSSGVNYSQITGCSVVKP 60

Query: 61  INGESKRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIA 120
           +N +SKR++SL P  A TL AEVAEK E VDYDGLA+DLENASPLEIMD+ALEKFG+DIA
Sbjct: 61  MNAQSKRDDSLVPFVANTLAAEVAEKTEIVDYDGLARDLENASPLEIMDQALEKFGDDIA 120

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE
Sbjct: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQ 240
           VQGLVRNKGLFSFY+DGHQECCRVRKVRPLRRALKG RAWITGQRKDQSPGTRS++PVVQ
Sbjct: 181 VQGLVRNKGLFSFYDDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSDVPVVQ 240

Query: 241 VDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDP FEGLDGGIGSLIKWNPVANV GKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV
Sbjct: 241 VDPVFEGLDGGIGSLIKWNPVANVHGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300

Query: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNR 360
           LPGQHEREGRWWWEDSKAKECGLHKGNLKQ  V QLNGNGN  PPVN+IFISQ+LVNLNR
Sbjct: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQGDVPQLNGNGNEIPPVNDIFISQDLVNLNR 360

Query: 361 TGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKE 420
           TG+ENLARL+NRKEPWLVVLYAPWCQFCQAMEGSYVELAEKL GSGVKVGKFRADGEQK+
Sbjct: 361 TGMENLARLDNRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLAGSGVKVGKFRADGEQKQ 420

Query: 421 FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDV+SLMAFVNALR
Sbjct: 421 FAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVESLMAFVNALR 465

BLAST of Tan0000931 vs. TAIR 10
Match: AT4G04610.1 (APS reductase 1 )

HSP 1 Score: 685.6 bits (1768), Expect = 2.7e-197
Identity = 341/466 (73.18%), Postives = 392/466 (84.12%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYS-LRGCSLKRING 60
           M++  SSS+ S      F  S E K  +IGS++L +R +V    +  S  R  S+K +N 
Sbjct: 3   MSVNVSSSSSSGIINSRFGVSLEPKVSQIGSLRLLDRVHVAPVSLNLSGKRSSSVKPLNA 62

Query: 61  ESKRNESLAPLAAITLGAEVAEKAETV---DYDGLAKDLENASPLEIMDKALEKFGNDIA 120
           E K  +S+ PLAA T+ AE+AE+ E V   D++ LAK LENASPLEIMDKALEK+GNDIA
Sbjct: 63  EPKTKDSMIPLAA-TMVAEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGNDIA 122

Query: 121 IAFSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVE 180
           IAFSGAEDVALIEYA LTGRPFRVFSLDTGRLN ETY+FFDAVEKHYGI IEYMFPD+VE
Sbjct: 123 IAFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETYRFFDAVEKHYGIRIEYMFPDSVE 182

Query: 181 VQGLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQ 240
           VQGLVR+KGLFSFYEDGHQECCRVRKVRPLRRALKG +AWITGQRKDQSPGTRSEIPVVQ
Sbjct: 183 VQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPVVQ 242

Query: 241 VDPAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPV 300
           VDP FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GY+SIGCEPCT+ V
Sbjct: 243 VDPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCTKAV 302

Query: 301 LPGQHEREGRWWWEDSKAKECGLHKGNLKQEG-VAQLNGNGNGAPPVNNIFISQNLVNLN 360
           LPGQHEREGRWWWED+KAKECGLHKGN+K+    A++NG    A  V +IF S+NLV L+
Sbjct: 303 LPGQHEREGRWWWEDAKAKECGLHKGNVKENSDDAKVNGESKSA--VADIFKSENLVTLS 362

Query: 361 RTGIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQK 420
           R GIENL +LENRKEPW+VVLYAPWC FCQAME SY ELA+KL GSG+KV KFRADG+QK
Sbjct: 363 RQGIENLMKLENRKEPWIVVLYAPWCPFCQAMEASYDELADKLAGSGIKVAKFRADGDQK 422

Query: 421 EFAKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           EFAKQELQLGSFPTIL FPK+SS+ IKYPSEKRDV+SL +F+N +R
Sbjct: 423 EFAKQELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465

BLAST of Tan0000931 vs. TAIR 10
Match: AT4G21990.1 (APS reductase 3 )

HSP 1 Score: 684.5 bits (1765), Expect = 5.9e-197
Identity = 338/461 (73.32%), Postives = 386/461 (83.73%), Query Frame = 0

Query: 4   ASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGESKR 63
           +SSSSAIS+S+      SS+ K  +IGS++L NR NV +A +  S +  S+K +N +S  
Sbjct: 9   SSSSSAISSSSF----PSSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKALNVQSIT 68

Query: 64  NESLAPLAAITLGAEVAEKAETV---DYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 123
            ES+       + +EV EK + V   D++ LAK LENASPLEIMDKALEKFGNDIAIAFS
Sbjct: 69  KESI-------VASEVTEKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAIAFS 128

Query: 124 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 183
           GAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEVQ L
Sbjct: 129 GAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEVQAL 188

Query: 184 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 243
           VRNKGLFSFYEDGHQECCR+RKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 189 VRNKGLFSFYEDGHQECCRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 248

Query: 244 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 303
           FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VLPGQ
Sbjct: 249 FEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVLPGQ 308

Query: 304 HEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAPPVNNIFISQNLVNLNRTGIE 363
           HEREGRWWWED+KAKECGLHKGN+K+        N NG   V +IF S+N+VNL+R GIE
Sbjct: 309 HEREGRWWWEDAKAKECGLHKGNIKENTNGNATANVNGTASVADIFNSENVVNLSRQGIE 368

Query: 364 NLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFAKQ 423
           NL +LENRKE W+VVLYAPWC FCQAME S+ ELA+KLGGSGVKV KFRADG+QK+FAK+
Sbjct: 369 NLMKLENRKEAWIVVLYAPWCPFCQAMEASFDELADKLGGSGVKVAKFRADGDQKDFAKK 428

Query: 424 ELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           ELQLGSFPTIL FPK+SS+ IKYPSEKRDVDSL +F+N +R
Sbjct: 429 ELQLGSFPTILVFPKNSSRPIKYPSEKRDVDSLTSFLNLVR 458

BLAST of Tan0000931 vs. TAIR 10
Match: AT1G62180.1 (5'adenylylphosphosulfate reductase 2 )

HSP 1 Score: 675.6 bits (1742), Expect = 2.8e-194
Identity = 339/464 (73.06%), Postives = 386/464 (83.19%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           +A+ SSS+AIS S+     ASSE+KA +I S++L +R ++       S R  S+K +N E
Sbjct: 3   LAVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHL-------SQRRYSMKPLNAE 62

Query: 61  S-KRNESLAPLAAITLGAEVAEK-AETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIA 120
           S  R+ES    A+  +  EV EK  E  D++ LAK LE+ASPLEIMDKALE+FG+ IAIA
Sbjct: 63  SHSRSESWVTRASTLIAPEVEEKGGEVEDFEQLAKKLEDASPLEIMDKALERFGDQIAIA 122

Query: 121 FSGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQ 180
           FSGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAVEVQ
Sbjct: 123 FSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAVEVQ 182

Query: 181 GLVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVD 240
            LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKG +AWITGQRKDQSPGTRSEIP+VQVD
Sbjct: 183 ALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIVQVD 242

Query: 241 PAFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLP 300
           P FEGLDGG+GSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRPVLP
Sbjct: 243 PVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPVLP 302

Query: 301 GQHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAP-PVNNIFISQNLVNLNRT 360
           GQHEREGRWWWED+KAKECGLHKGN+K+E     +G  +  P  V  IF S N+V L++ 
Sbjct: 303 GQHEREGRWWWEDAKAKECGLHKGNIKEE-----DGAADSKPAAVQEIFESNNVVALSKG 362

Query: 361 GIENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEF 420
           G+ENL +LENRKE WLVVLYAPWC FCQAME SY+ELAEKL G GVKV KFRADGEQKEF
Sbjct: 363 GVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADGEQKEF 422

Query: 421 AKQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           AKQELQLGSFPTIL FPK + +AIKYPSE RDVDSLM+FVN LR
Sbjct: 423 AKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 454

BLAST of Tan0000931 vs. TAIR 10
Match: AT1G62180.2 (5'adenylylphosphosulfate reductase 2 )

HSP 1 Score: 668.7 bits (1724), Expect = 3.4e-192
Identity = 336/463 (72.57%), Postives = 383/463 (82.72%), Query Frame = 0

Query: 1   MALASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGE 60
           +A+ SSS+AIS S+     ASSE+KA +I S++L +R ++       S R  S+K +N E
Sbjct: 3   LAVTSSSTAISGSSFSRSGASSESKALQICSIRLSDRTHL-------SQRRYSMKPLNAE 62

Query: 61  S-KRNESLAPLAAITLGAEVAEKAETVDYDGLAKDLENASPLEIMDKALEKFGNDIAIAF 120
           S  R+ES    A+  +  EV       D++ LAK LE+ASPLEIMDKALE+FG+ IAIAF
Sbjct: 63  SHSRSESWVTRASTLIAPEVE------DFEQLAKKLEDASPLEIMDKALERFGDQIAIAF 122

Query: 121 SGAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQG 180
           SGAEDVALIEYARLTG+PFRVFSLDTGRLN ETY+ FDAVEK YGI IEYMFPDAVEVQ 
Sbjct: 123 SGAEDVALIEYARLTGKPFRVFSLDTGRLNPETYRLFDAVEKQYGIRIEYMFPDAVEVQA 182

Query: 181 LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDP 240
           LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKG +AWITGQRKDQSPGTRSEIP+VQVDP
Sbjct: 183 LVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIPIVQVDP 242

Query: 241 AFEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPG 300
            FEGLDGG+GSL+KWNP+ANV+G D+WNFLRTM+VPVN+LH+QGYVSIGCEPCTRPVLPG
Sbjct: 243 VFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPVLPG 302

Query: 301 QHEREGRWWWEDSKAKECGLHKGNLKQEGVAQLNGNGNGAP-PVNNIFISQNLVNLNRTG 360
           QHEREGRWWWED+KAKECGLHKGN+K+E     +G  +  P  V  IF S N+V L++ G
Sbjct: 303 QHEREGRWWWEDAKAKECGLHKGNIKEE-----DGAADSKPAAVQEIFESNNVVALSKGG 362

Query: 361 IENLARLENRKEPWLVVLYAPWCQFCQAMEGSYVELAEKLGGSGVKVGKFRADGEQKEFA 420
           +ENL +LENRKE WLVVLYAPWC FCQAME SY+ELAEKL G GVKV KFRADGEQKEFA
Sbjct: 363 VENLLKLENRKEAWLVVLYAPWCPFCQAMEASYIELAEKLAGKGVKVAKFRADGEQKEFA 422

Query: 421 KQELQLGSFPTILFFPKHSSKAIKYPSEKRDVDSLMAFVNALR 462
           KQELQLGSFPTIL FPK + +AIKYPSE RDVDSLM+FVN LR
Sbjct: 423 KQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 447

BLAST of Tan0000931 vs. TAIR 10
Match: AT4G21990.2 (APS reductase 3 )

HSP 1 Score: 454.5 bits (1168), Expect = 1.0e-127
Identity = 228/307 (74.27%), Postives = 257/307 (83.71%), Query Frame = 0

Query: 4   ASSSSAISASNLQHFAASSEAKAPRIGSVQLFNRPNVLSAGVRYSLRGCSLKRINGESKR 63
           +SSSSAIS+S+      SS+ K  +IGS++L NR NV +A +  S +  S+K +N +S  
Sbjct: 44  SSSSSAISSSSF----PSSDLKVTKIGSLRLLNRTNVSAASLSLSGKRSSVKALNVQSIT 103

Query: 64  NESLAPLAAITLGAEVAEKAETV---DYDGLAKDLENASPLEIMDKALEKFGNDIAIAFS 123
            ES+       + +EV EK + V   D++ LAK LENASPLEIMDKALEKFGNDIAIAFS
Sbjct: 104 KESI-------VASEVTEKLDVVEVEDFEELAKRLENASPLEIMDKALEKFGNDIAIAFS 163

Query: 124 GAEDVALIEYARLTGRPFRVFSLDTGRLNSETYQFFDAVEKHYGIHIEYMFPDAVEVQGL 183
           GAEDVALIEYA LTGRP+RVFSLDTGRLN ETY+ FD VEKHYGI IEYMFPDAVEVQ L
Sbjct: 164 GAEDVALIEYAHLTGRPYRVFSLDTGRLNPETYRLFDTVEKHYGIRIEYMFPDAVEVQAL 223

Query: 184 VRNKGLFSFYEDGHQECCRVRKVRPLRRALKGFRAWITGQRKDQSPGTRSEIPVVQVDPA 243
           VRNKGLFSFYEDGHQECCR+RKVRPLRRALKG RAWITGQRKDQSPGTRSEIPVVQVDP 
Sbjct: 224 VRNKGLFSFYEDGHQECCRIRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIPVVQVDPV 283

Query: 244 FEGLDGGIGSLIKWNPVANVDGKDIWNFLRTMNVPVNSLHSQGYVSIGCEPCTRPVLPGQ 303
           FEGLDGG+GSL+KWNPVANV+G D+WNFLRTM+VPVN+LH+ GYVSIGCEPCTR VLPGQ
Sbjct: 284 FEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYVSIGCEPCTRAVLPGQ 339

Query: 304 HEREGRW 308
           HEREGRW
Sbjct: 344 HEREGRW 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929793.8e-19673.185'-adenylylsulfate reductase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
P929808.4e-19673.325'-adenylylsulfate reductase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
P929813.9e-19373.065'-adenylylsulfate reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q6Z4A72.6e-18167.64Probable 5'-adenylylsulfate reductase 1, chloroplastic OS=Oryza sativa subsp. ja... [more]
B7VBC36.4e-7954.13Adenosine 5'-phosphosulfate reductase OS=Pseudomonas aeruginosa (strain LESB58) ... [more]
Match NameE-valueIdentityDescription
XP_038895661.11.7e-24190.525'-adenylylsulfate reductase 3, chloroplastic-like [Benincasa hispida][more]
XP_022923952.11.3e-23889.155'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita moschata] >KAG7019... [more]
KAG6584094.12.2e-23889.155'-adenylylsulfate reductase 3, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_023520098.15.0e-23889.375'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023001259.13.2e-23788.725'-adenylylsulfate reductase 3, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E7I76.3e-23989.155'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1KM851.6e-23788.725'-adenylylsulfate reductase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 G... [more]
A0A5A7UP262.3e-23688.395'-adenylylsulfate reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
E5GC592.3e-23688.39Adenylyl-sulfate reductase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3B2C82.3e-23688.395'-adenylylsulfate reductase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT4G04610.12.7e-19773.18APS reductase 1 [more]
AT4G21990.15.9e-19773.32APS reductase 3 [more]
AT1G62180.12.8e-19473.065'adenylylphosphosulfate reductase 2 [more]
AT1G62180.23.4e-19272.575'adenylylphosphosulfate reductase 2 [more]
AT4G21990.21.0e-12774.27APS reductase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 364..458
e-value: 1.4E-11
score: 44.2
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 333..461
score: 11.456102
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 347..459
e-value: 6.3E-19
score: 70.0
NoneNo IPR availablePANTHERPTHR46482:SF85'-ADENYLYLSULFATE REDUCTASE 1, CHLOROPLASTICcoord: 1..461
NoneNo IPR availablePANTHERPTHR464825'-ADENYLYLSULFATE REDUCTASE 3, CHLOROPLASTICcoord: 1..461
NoneNo IPR availableCDDcd02993PDI_a_APS_reductasecoord: 350..457
e-value: 6.26387E-64
score: 199.986
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 88..283
IPR002500Phosphoadenosine phosphosulphate reductasePFAMPF01507PAPS_reductcoord: 115..295
e-value: 2.6E-44
score: 151.3
IPR002500Phosphoadenosine phosphosulphate reductaseCDDcd01713PAPS_reductasecoord: 114..288
e-value: 9.84286E-55
score: 178.367
IPR004508Thioredoxin-independent 5'-adenylylsulphate reductaseTIGRFAMTIGR00424TIGR00424coord: 1..461
e-value: 3.2E-263
score: 871.2
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 86..310
e-value: 2.3E-71
score: 241.8
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 350..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000931.1Tan0000931.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0019419 sulfate reduction
molecular_function GO:0004604 phosphoadenylyl-sulfate reductase (thioredoxin) activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016671 oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor