Tan0000891 (gene) Snake gourd v1

Overview
NameTan0000891
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description1-aminocyclopropane-1-carboxylate synthase
LocationLG01: 14465895 .. 14467728 (-)
RNA-Seq ExpressionTan0000891
SyntenyTan0000891
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGATATTGTCTCGAAAAGCCACGTGCAACTCACATGGACAAGATTCGTCTTACTTCTTAGGCTGGGAAGAATATGAGAAGAATCCTTACGATGAGATTCAAAATCCTAAAGGGATTATTCAAATGGGTCTTGCAGAAAATCAGGTTGGTATGGTATCCTATAATAATAACAATCTAAAAATTATATAATAATGTTAGATATTGTTGTTATATTTTGAATTAATTAACGTTAATTTTGAATGAATGTCTCGTCACAGCTATGTTTTGACCTGCTGGAGAGGTGGCTGGAGGAGAATCCAGATGCGGCTGGTTTTAAAAGAGATGGCAAGTCCATTTTCAGAGAACTTGCTCTCTTTCAAGATTACAATGGTTTGCCTGCATTCAAAAAGGTATTGTCTTTGTCTTTATCTTTAATTTTTAATATTATTTATATTGTATGTGACATCAATTTAATTTGGGATATAGATGATCCTAACTTTTATTGTTTAATAATGTTTCAGGCATTGTCAGAGTTCATGTCGGAAATTAGAGGGAACAAAGTAACGTTCGATCCCAACCACATAGTTCTCACTGCTGGTGCCACGTCAGCTAATGAGACTCTTATGTTCTGCTTGGCTGAACCTGGCGATGCCTTTCTTCTACCTACCCCGTACTATCCAGGGTACGTATTTAAAAACAGAATTGTATCTCTTTAGTCTCTGTATTTAACCGACATGAATTATTAAACACAAAATTGTAAAAAGTTTTCGACTTAATTAGAGATAGATGTACCATTGCATGGCATGTTATGAAAAAATAATGAGTTCTCTACACTTTGCAACACCTAACGTTGGTTTTTTTGGTTTTGCTTTTGTACAGATTTGATAGAGATTTGAAATGGAGAACTGGGATTGAAATCGTACCCATCCATTGCTCGAGCTCCAATGGCTTCAAAATCACAGAGGCAGCACTGTTGGAAGCTTACCAAAACGCCCAAAATCGTAACCTCAAGGTCAAAGGCGTACTGGTGACAAACCCATCAAACCCATTAGGAACCACCATCAGCACTCAAGAACTCAACCTCCTACTCCAATTCATTGCATCAAAAAATCTCCACCTAATCAGCGACGAGATCTACTCGGGCACAGTTTTCCGCAACCCGGGCTTCGTTAGCGCCATGGAAATTCTCAACCAAAACTACAAACACAACCACCAAATCCGTAAACAAGTTCACATTGTTTACAGTCTCTCGAAAGATCTCGGCCTACCGGGGTTCCGCGTCGGAGCCATTTATTCGAACGACGACGTCGTCGTGGCTGCAGCGACGAAGATGTCGAGCTTTGGATTGGTGTCGTCGCAAACGCAGTACCTTTTATCGGCCATGTTATCGAACAAGAAGTTTACGAGGAATTACATTAGGGAGAATCAAAAGAGGCTAGAGAAAAGGCAGAGAATGCTGGTGAGAGGGCTGCAAAAGGCCGGGATTAAGTGCCACAAAGGGAATGCAGGGTTGTTTTGCTGGGTGGATATGAGGCATTTGTTGAGAACCAATACTTTTGAATCGGAAATGGAGCTTTGGAGGAAGATTGTGTTCGAGGTGAGGTTAAACGTGTCGCCCGGACAGTCGTGCCACTGCTCCGAACCCGGTTGGTTTCGTGTTTGTTTTGCCAACATGTCCGAGGCCACATTGGACCTCGCAATGCAGAGATTGAAGGCTTTTGTGCTGGACTCTACTCTAACTACTGCTGTCATTGCTACTAGAAACCAACTCGACACAAAGAGGAAGTCGATGGCTAAATGGGTTTTTCGGTTATCGTTTCACGATCGCGGTTGCGAACCACGTGATCGATAG

mRNA sequence

ATGGGGATATTGTCTCGAAAAGCCACGTGCAACTCACATGGACAAGATTCGTCTTACTTCTTAGGCTGGGAAGAATATGAGAAGAATCCTTACGATGAGATTCAAAATCCTAAAGGGATTATTCAAATGGGTCTTGCAGAAAATCAGCTATGTTTTGACCTGCTGGAGAGGTGGCTGGAGGAGAATCCAGATGCGGCTGGTTTTAAAAGAGATGGCAAGTCCATTTTCAGAGAACTTGCTCTCTTTCAAGATTACAATGGTTTGCCTGCATTCAAAAAGGCATTGTCAGAGTTCATGTCGGAAATTAGAGGGAACAAAGTAACGTTCGATCCCAACCACATAGTTCTCACTGCTGGTGCCACGTCAGCTAATGAGACTCTTATGTTCTGCTTGGCTGAACCTGGCGATGCCTTTCTTCTACCTACCCCGTACTATCCAGGATTTGATAGAGATTTGAAATGGAGAACTGGGATTGAAATCGTACCCATCCATTGCTCGAGCTCCAATGGCTTCAAAATCACAGAGGCAGCACTGTTGGAAGCTTACCAAAACGCCCAAAATCGTAACCTCAAGGTCAAAGGCGTACTGGTGACAAACCCATCAAACCCATTAGGAACCACCATCAGCACTCAAGAACTCAACCTCCTACTCCAATTCATTGCATCAAAAAATCTCCACCTAATCAGCGACGAGATCTACTCGGGCACAGTTTTCCGCAACCCGGGCTTCGTTAGCGCCATGGAAATTCTCAACCAAAACTACAAACACAACCACCAAATCCGTAAACAAGTTCACATTGTTTACAGTCTCTCGAAAGATCTCGGCCTACCGGGGTTCCGCGTCGGAGCCATTTATTCGAACGACGACGTCGTCGTGGCTGCAGCGACGAAGATGTCGAGCTTTGGATTGGTGTCGTCGCAAACGCAGTACCTTTTATCGGCCATGTTATCGAACAAGAAGTTTACGAGGAATTACATTAGGGAGAATCAAAAGAGGCTAGAGAAAAGGCAGAGAATGCTGGTGAGAGGGCTGCAAAAGGCCGGGATTAAGTGCCACAAAGGGAATGCAGGGTTGTTTTGCTGGGTGGATATGAGGCATTTGTTGAGAACCAATACTTTTGAATCGGAAATGGAGCTTTGGAGGAAGATTGTGTTCGAGGTGAGGTTAAACGTGTCGCCCGGACAGTCGTGCCACTGCTCCGAACCCGGTTGGTTTCGTGTTTGTTTTGCCAACATGTCCGAGGCCACATTGGACCTCGCAATGCAGAGATTGAAGGCTTTTGTGCTGGACTCTACTCTAACTACTGCTGTCATTGCTACTAGAAACCAACTCGACACAAAGAGGAAGTCGATGGCTAAATGGGTTTTTCGGTTATCGTTTCACGATCGCGGTTGCGAACCACGTGATCGATAG

Coding sequence (CDS)

ATGGGGATATTGTCTCGAAAAGCCACGTGCAACTCACATGGACAAGATTCGTCTTACTTCTTAGGCTGGGAAGAATATGAGAAGAATCCTTACGATGAGATTCAAAATCCTAAAGGGATTATTCAAATGGGTCTTGCAGAAAATCAGCTATGTTTTGACCTGCTGGAGAGGTGGCTGGAGGAGAATCCAGATGCGGCTGGTTTTAAAAGAGATGGCAAGTCCATTTTCAGAGAACTTGCTCTCTTTCAAGATTACAATGGTTTGCCTGCATTCAAAAAGGCATTGTCAGAGTTCATGTCGGAAATTAGAGGGAACAAAGTAACGTTCGATCCCAACCACATAGTTCTCACTGCTGGTGCCACGTCAGCTAATGAGACTCTTATGTTCTGCTTGGCTGAACCTGGCGATGCCTTTCTTCTACCTACCCCGTACTATCCAGGATTTGATAGAGATTTGAAATGGAGAACTGGGATTGAAATCGTACCCATCCATTGCTCGAGCTCCAATGGCTTCAAAATCACAGAGGCAGCACTGTTGGAAGCTTACCAAAACGCCCAAAATCGTAACCTCAAGGTCAAAGGCGTACTGGTGACAAACCCATCAAACCCATTAGGAACCACCATCAGCACTCAAGAACTCAACCTCCTACTCCAATTCATTGCATCAAAAAATCTCCACCTAATCAGCGACGAGATCTACTCGGGCACAGTTTTCCGCAACCCGGGCTTCGTTAGCGCCATGGAAATTCTCAACCAAAACTACAAACACAACCACCAAATCCGTAAACAAGTTCACATTGTTTACAGTCTCTCGAAAGATCTCGGCCTACCGGGGTTCCGCGTCGGAGCCATTTATTCGAACGACGACGTCGTCGTGGCTGCAGCGACGAAGATGTCGAGCTTTGGATTGGTGTCGTCGCAAACGCAGTACCTTTTATCGGCCATGTTATCGAACAAGAAGTTTACGAGGAATTACATTAGGGAGAATCAAAAGAGGCTAGAGAAAAGGCAGAGAATGCTGGTGAGAGGGCTGCAAAAGGCCGGGATTAAGTGCCACAAAGGGAATGCAGGGTTGTTTTGCTGGGTGGATATGAGGCATTTGTTGAGAACCAATACTTTTGAATCGGAAATGGAGCTTTGGAGGAAGATTGTGTTCGAGGTGAGGTTAAACGTGTCGCCCGGACAGTCGTGCCACTGCTCCGAACCCGGTTGGTTTCGTGTTTGTTTTGCCAACATGTCCGAGGCCACATTGGACCTCGCAATGCAGAGATTGAAGGCTTTTGTGCTGGACTCTACTCTAACTACTGCTGTCATTGCTACTAGAAACCAACTCGACACAAAGAGGAAGTCGATGGCTAAATGGGTTTTTCGGTTATCGTTTCACGATCGCGGTTGCGAACCACGTGATCGATAG

Protein sequence

MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLEENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGATSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLEAYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRNPGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSSFGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFCWVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMQRLKAFVLDSTLTTAVIATRNQLDTKRKSMAKWVFRLSFHDRGCEPRDR
Homology
BLAST of Tan0000891 vs. ExPASy Swiss-Prot
Match: Q42881 (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=ACS3 PE=1 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 5.7e-208
Identity = 352/466 (75.54%), Postives = 406/466 (87.12%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           M +LS KATCNSHGQDSSYFLGW+EYEKNPYDEIQNPKGIIQMGLAENQL FDLLE WL 
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           +NPDAAGFKR+G+SIFRELALFQDY+GLPAFK A+++FMSEIRGN+V+FD N++VLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLA  GDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITE+AL E
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AY +A+ RNLKVKGVLVTNPSNPLGTT++  EL LLL FI  K +HLISDEIYSGTVF +
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS ME+L +      ++ ++VHIVYSLSKDLGLPGFR+GAIYSND++VV+AATKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLS MLS+KKFT+ YI ENQKRL+KR  MLV+GL+ AGI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL +N F++EM+LW+KIV++V LN+SPG SCHC+EPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MQRLKAFVLDSTLTTAVIATRNQ---LDTKRKSMAKWVFRLSFHDR 464
           M+R+K FV +ST   A      Q    ++K+KS +KWVFRLSF+DR
Sbjct: 421 MRRIKDFV-ESTAPNATNHQNQQQSNANSKKKSFSKWVFRLSFNDR 465

BLAST of Tan0000891 vs. ExPASy Swiss-Prot
Match: P37821 (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 715.3 bits (1845), Expect = 4.5e-205
Identity = 345/471 (73.25%), Postives = 403/471 (85.56%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           M +LSR AT NSHGQDSSYFLGW+EYEKNPY E+ N  GIIQMGLAENQLCFDLLE WL 
Sbjct: 1   MRMLSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           +NP+AA FK++G+SIF ELALFQDY+GLPAFKKA+ +FM+EIRGNKVTFDPNH+VLTAGA
Sbjct: 61  KNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANET +FCLA+PG+A L+PTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+ITE AL E
Sbjct: 121 TSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ A+ RNL+VKGVLVTNPSNPLGTT++  EL LLL F+  K +HLISDEIYSGT F +
Sbjct: 181 AYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSS 240

Query: 241 PGFVSAMEIL-NQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMS 300
           P F+S ME+L ++N   N ++ ++VH+VYSLSKDLGLPGFRVGAIYSNDD+VVAAATKMS
Sbjct: 241 PSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLF 360
           SFGLVSSQTQ+LLSAMLS+KK T+NYI EN KRL++RQ+ LV GLQK+GI C  GNAGLF
Sbjct: 301 SFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLF 360

Query: 361 CWVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDL 420
           CWVDMRHLLR+NTFE+EMELW+KIV+EV LN+SPG SCHC+EPGWFRVCFAN+ E TLDL
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDL 420

Query: 421 AMQRLKAFVLDSTLTTAV---IATRNQLDTKRKSMAKWVFRLSFHDRGCEP 468
           AMQRLKAFV +      V     + +   ++R+S+ KWV RLSF DRG  P
Sbjct: 421 AMQRLKAFVGEYYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIP 471

BLAST of Tan0000891 vs. ExPASy Swiss-Prot
Match: Q00257 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.0e-204
Identity = 351/463 (75.81%), Postives = 401/463 (86.61%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           M +LS KATCNSHGQDSSYFLGWE YE NP+    NP GIIQMGLAENQL FDLLE WL 
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           +NPDAA FKRDGKSIFRELALFQDY+GLPAFKKAL EFM+EIRGNKV+F+ N+IVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG+EIVPIHC+SSNGF+IT++AL +
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AY++AQ RNL+VKGVLVTNPSNPLGTT++  ELNL+  FI SK +HLISDEIYSGTVF +
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVSAME+L +    + ++ K+VHIVYSLSKDLGLPGFRVGAIYSNDD+VVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT +YI ENQKRL++RQ+MLV GLQKAGI C   NAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL ++ FESE+ELW+KIV+EV LN+SPG SCHC+EPGWFRVCFANMSE+TL LA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 MQRLKAFV--LDSTLTTAVIATRNQL--DTKRKSMAKWVFRLS 460
           ++RLK+FV  L ST T+      N++  + K+    KWVFR S
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQS 463

BLAST of Tan0000891 vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 2.9e-204
Identity = 345/463 (74.51%), Postives = 402/463 (86.83%), Query Frame = 0

Query: 4   LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLEENP 63
           LSRK T N+HGQDSSYFLGWEEYEKNPYDEI+NP GIIQMGLAENQLCFDL+E WL +NP
Sbjct: 4   LSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLAKNP 63

Query: 64  DAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGATSA 123
           DAAG K+DG+SIF+ELALFQDY+GLP FKKAL+EFM EIRGN+VTFDP+ IVL AG+TSA
Sbjct: 64  DAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGSTSA 123

Query: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLEAYQ 183
           NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITE+AL +AYQ
Sbjct: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQAYQ 183

Query: 184 NAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRNPGF 243
            AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGTVF    F
Sbjct: 184 QAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGFEQF 243

Query: 244 VSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSSFGL 303
           VS M++L      N ++ K+VHIVYSLSKDLGLPGFRVGAIYSND++VV+AATKMSSFGL
Sbjct: 244 VSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSSFGL 303

Query: 304 VSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFCWVD 363
           VSSQTQYLLSA+LS+KKFT  Y+ ENQKRL+ RQ+ LV GL+ AGI C K NAGLFCWVD
Sbjct: 304 VSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFCWVD 363

Query: 364 MRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMQR 423
           MRHLL TNTFE+E+ELW+KIV++V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLAM+R
Sbjct: 364 MRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLAMKR 423

Query: 424 LKAFVLDSTLTTAVIATRNQ---LDTKRKSMAKWVFRLSFHDR 464
           LK +V +ST +  VI+  +       ++++++ WVFR+S+ DR
Sbjct: 424 LKEYV-ESTDSRRVISKSSHDRIKSLRKRTVSNWVFRVSWTDR 465

BLAST of Tan0000891 vs. ExPASy Swiss-Prot
Match: Q9T065 (1-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=ACS8 PE=1 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 1.8e-201
Identity = 337/471 (71.55%), Postives = 397/471 (84.29%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MG+LS+KA+CN+HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQL FDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           +NPDAA F+R+G+SIFRELALFQDY+GLP+FK A+++FMSE RGN+V+F+PN +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           T ANETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG EIVPI C S+NGF+IT+ AL E
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AY+ AQ  NLKVKGVL+TNPSNPLGTT +  ELN LL FI+ K +HLISDEIYSGTVF N
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGF+S ME+L      N  +  +VHIVYSLSKDLGLPGFRVG IYSNDD VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGL+SSQTQYLLSA+LS+K FT+NY+ ENQ RL+ R + LV GL+ AGI+C K NAGLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL++NTFE+E+ELW+KIV+EV+LN+SPG SCHC+EPGWFRVCFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 MQRLKAFVLDSTLTTAVIATRNQLDTKRK-SMAKWVFRLSFHDRGCEPRDR 471
           + RLK FV   + T    +   +L   RK  ++ WVFRLSFHDR  EP +R
Sbjct: 421 LDRLKRFVDGPSPTRRSQSEHQRLKNLRKMKVSNWVFRLSFHDR--EPEER 469

BLAST of Tan0000891 vs. NCBI nr
Match: XP_022943238.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata])

HSP 1 Score: 884.4 bits (2284), Expect = 4.3e-253
Identity = 432/465 (92.90%), Postives = 452/465 (97.20%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKAL+EFMSEIRGN+VTF+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVFRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MQRLKAFV+DST+TT+     T N L TKRK+M KWVFRLSF+DR
Sbjct: 421 MQRLKAFVVDSTVTTSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

BLAST of Tan0000891 vs. NCBI nr
Match: XP_023522256.1 (1-aminocyclopropane-1-carboxylate synthase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.3 bits (2276), Expect = 3.6e-252
Identity = 430/465 (92.47%), Postives = 452/465 (97.20%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKAL+EFMSEIRGN+VTF+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVFRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MQRLKAFV++ST+TT+     T N L TK+K+M KWVFRLSF+DR
Sbjct: 421 MQRLKAFVVNSTVTTSPTTTTTTNHLPTKKKNMVKWVFRLSFNDR 465

BLAST of Tan0000891 vs. NCBI nr
Match: KAG6600672.1 (1-aminocyclopropane-1-carboxylate synthase 101, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 430/465 (92.47%), Postives = 451/465 (96.99%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKAL+EFMSEIRGN+VTF+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVFRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSND+VVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDNVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MQRLKAFV+DST+TT+     T   L TKRK+M KWVFRLSF+DR
Sbjct: 421 MQRLKAFVVDSTVTTSPTTTTTTYHLPTKRKNMVKWVFRLSFNDR 465

BLAST of Tan0000891 vs. NCBI nr
Match: XP_022985152.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita maxima])

HSP 1 Score: 879.0 bits (2270), Expect = 1.8e-251
Identity = 430/465 (92.47%), Postives = 450/465 (96.77%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKAL+EFMSEIRGN+VTF+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVFRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MQRLKAFV+ ST+ T+     T N L TKRK+M KWVFRLSF+DR
Sbjct: 421 MQRLKAFVVYSTVATSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

BLAST of Tan0000891 vs. NCBI nr
Match: KAG7031311.1 (1-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 876.7 bits (2264), Expect = 8.9e-251
Identity = 432/474 (91.14%), Postives = 452/474 (95.36%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKK---------ALSEFMSEIRGNKVTFDP 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKK         AL+EFMSEIRGN+VTF+P
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKLDLIQCAFQALAEFMSEIRGNRVTFNP 120

Query: 121 NHIVLTAGATSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF 180
           +HIVLTAGATSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF
Sbjct: 121 DHIVLTAGATSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF 180

Query: 181 KITEAALLEAYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDE 240
           +ITE+ALLEAYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDE
Sbjct: 181 RITESALLEAYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDE 240

Query: 241 IYSGTVFRNPGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVV 300
           IYSGTVFRNPGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVV
Sbjct: 241 IYSGTVFRNPGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVV 300

Query: 301 VAAATKMSSFGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKC 360
           VAAATKMSSFGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKC
Sbjct: 301 VAAATKMSSFGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKC 360

Query: 361 HKGNAGLFCWVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFAN 420
           HKGNAGLFCWVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFAN
Sbjct: 361 HKGNAGLFCWVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFAN 420

Query: 421 MSEATLDLAMQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MSEATLDLAMQRLKAFV+DST+TT+     T N L TKRK+M KWVFRLSF+DR
Sbjct: 421 MSEATLDLAMQRLKAFVVDSTVTTSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 474

BLAST of Tan0000891 vs. ExPASy TrEMBL
Match: A0A6J1FWL0 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111448029 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.1e-253
Identity = 432/465 (92.90%), Postives = 452/465 (97.20%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKAL+EFMSEIRGN+VTF+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVFRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MQRLKAFV+DST+TT+     T N L TKRK+M KWVFRLSF+DR
Sbjct: 421 MQRLKAFVVDSTVTTSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

BLAST of Tan0000891 vs. ExPASy TrEMBL
Match: A0A6J1J434 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111483239 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 8.7e-252
Identity = 430/465 (92.47%), Postives = 450/465 (96.77%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MGILSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQLCFDLLERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKAL+EFMSEIRGN+VTF+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGF+ITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVFRN
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGFVS MEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKFT+NYI+ENQKRLEKRQ+MLVRGLQKAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA--VIATRNQLDTKRKSMAKWVFRLSFHDR 464
           MQRLKAFV+ ST+ T+     T N L TKRK+M KWVFRLSF+DR
Sbjct: 421 MQRLKAFVVYSTVATSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

BLAST of Tan0000891 vs. ExPASy TrEMBL
Match: A0A6J1ENA4 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111435906 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.3e-244
Identity = 424/479 (88.52%), Postives = 445/479 (92.90%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEYEKNPYDEI+NPKGIIQMGLAENQLCFDL+ERWLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIF+ELALFQDYNGLPAFKKAL+EFMSEIR NKVTFDP+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPI CSSSNGFKIT+ ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFI SKNLHLISDEIYSGTVF+ 
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIVSKNLHLISDEIYSGTVFQT 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           P FVS MEILNQNYKHN QIRKQVHIVYSLSKDLGLPGFRVGA+YSND+VVVAAATKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS++ F R YIRENQ RL+KRQRMLV GL+KAGIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFE+EMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDST---LTTAVI------ATRNQLDTKRKSMAKWVFRLSFHDRGCEPRDR 471
           M+R KAFV DST   L ++ I      +T NQLDTKRKS+AKWVFRLS HDR  E RDR
Sbjct: 421 MKRFKAFVADSTTVKLNSSRIRSRNTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 479

BLAST of Tan0000891 vs. ExPASy TrEMBL
Match: A0A6J1J417 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111483220 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.2e-242
Identity = 418/479 (87.27%), Postives = 439/479 (91.65%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MG+LS KATCNSHGQDSSYFLGWEEYEKNPY EI+NP GIIQMGLAENQLCFDL+ERWLE
Sbjct: 1   MGLLSTKATCNSHGQDSSYFLGWEEYEKNPYHEIRNPNGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIF+ELALFQDYNGLPAFKKAL+EFMSEIR NKVTFDP+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPI CSSSNGFKIT+ ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQ+AQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVF+ 
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           P FVS MEILNQ+YKHN QIRKQVHIVYSLSKDLGLPGFRVGA+YSND+VVVAAATKMSS
Sbjct: 241 PAFVSVMEILNQSYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLS MLS++ F R YIRENQ RL+KRQRMLV GL+KAGIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSVMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFE+EMELWRKIVFEVRLNVSP QSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPSQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MQRLKAFVLDSTLTTA---------VIATRNQLDTKRKSMAKWVFRLSFHDRGCEPRDR 471
           M+R KAFV+DST T           V +T N LDTKRKS+AKWVFRLS HDR  E RDR
Sbjct: 421 MKRFKAFVVDSTTTKLNSTWIRSRNVNSTSNNLDTKRKSVAKWVFRLSIHDRSSESRDR 479

BLAST of Tan0000891 vs. ExPASy TrEMBL
Match: A0A0A0KVS1 (Aminotran_1_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G099220 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.2e-231
Identity = 394/437 (90.16%), Postives = 414/437 (94.74%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MG+LSRKATCN HGQDSSYF GWEEYEKNPYDEIQNPKGIIQMGLAENQLCFD++E+WLE
Sbjct: 1   MGLLSRKATCNLHGQDSSYFSGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDVVEKWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           ENP++AGFKRDGKSIFR+LALFQDYNGLPAFKKAL+EFMSEIRGNKV FDPNHIVLTAGA
Sbjct: 61  ENPNSAGFKRDGKSIFRQLALFQDYNGLPAFKKALAEFMSEIRGNKVAFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITE+ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITESALLE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AYQNA N NLKVKGVLVTNPSNPLGTTIS+ ELNLLLQFI SKNLHLISDEIYSGT FRN
Sbjct: 181 AYQNALNCNLKVKGVLVTNPSNPLGTTISSHELNLLLQFIESKNLHLISDEIYSGTAFRN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
            GFVSAMEILNQ YKHNHQI+ QVHIVYSLSKDLGLPGFRVGA+YSND VVVAAATKMSS
Sbjct: 241 SGFVSAMEILNQTYKHNHQIQNQVHIVYSLSKDLGLPGFRVGALYSNDKVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLS+KKF R YIR+NQKRLEKRQ MLV GL+KAGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFRRKYIRKNQKRLEKRQTMLVNGLEKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFESEMELWRKI+FEVRLNVSPGQSCHCSEPGWFR+CFANMSE TLDLA
Sbjct: 361 WVDMRHLLGGNTFESEMELWRKILFEVRLNVSPGQSCHCSEPGWFRICFANMSEPTLDLA 420

Query: 421 MQRLKAFVLDSTLTTAV 438
           MQRLKAFV++ + T  V
Sbjct: 421 MQRLKAFVVNYSTTITV 437

BLAST of Tan0000891 vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 712.6 bits (1838), Expect = 2.1e-205
Identity = 345/463 (74.51%), Postives = 402/463 (86.83%), Query Frame = 0

Query: 4   LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLEENP 63
           LSRK T N+HGQDSSYFLGWEEYEKNPYDEI+NP GIIQMGLAENQLCFDL+E WL +NP
Sbjct: 4   LSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLAKNP 63

Query: 64  DAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGATSA 123
           DAAG K+DG+SIF+ELALFQDY+GLP FKKAL+EFM EIRGN+VTFDP+ IVL AG+TSA
Sbjct: 64  DAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGSTSA 123

Query: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLEAYQ 183
           NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITE+AL +AYQ
Sbjct: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQAYQ 183

Query: 184 NAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRNPGF 243
            AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGTVF    F
Sbjct: 184 QAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGFEQF 243

Query: 244 VSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSSFGL 303
           VS M++L      N ++ K+VHIVYSLSKDLGLPGFRVGAIYSND++VV+AATKMSSFGL
Sbjct: 244 VSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSSFGL 303

Query: 304 VSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFCWVD 363
           VSSQTQYLLSA+LS+KKFT  Y+ ENQKRL+ RQ+ LV GL+ AGI C K NAGLFCWVD
Sbjct: 304 VSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFCWVD 363

Query: 364 MRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMQR 423
           MRHLL TNTFE+E+ELW+KIV++V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLAM+R
Sbjct: 364 MRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLAMKR 423

Query: 424 LKAFVLDSTLTTAVIATRNQ---LDTKRKSMAKWVFRLSFHDR 464
           LK +V +ST +  VI+  +       ++++++ WVFR+S+ DR
Sbjct: 424 LKEYV-ESTDSRRVISKSSHDRIKSLRKRTVSNWVFRVSWTDR 465

BLAST of Tan0000891 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 703.4 bits (1814), Expect = 1.3e-202
Identity = 337/471 (71.55%), Postives = 397/471 (84.29%), Query Frame = 0

Query: 1   MGILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLE 60
           MG+LS+KA+CN+HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQL FDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGA 120
           +NPDAA F+R+G+SIFRELALFQDY+GLP+FK A+++FMSE RGN+V+F+PN +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLE 180
           T ANETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG EIVPI C S+NGF+IT+ AL E
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYQNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240
           AY+ AQ  NLKVKGVL+TNPSNPLGTT +  ELN LL FI+ K +HLISDEIYSGTVF N
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300
           PGF+S ME+L      N  +  +VHIVYSLSKDLGLPGFRVG IYSNDD VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFC 360
           FGL+SSQTQYLLSA+LS+K FT+NY+ ENQ RL+ R + LV GL+ AGI+C K NAGLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL++NTFE+E+ELW+KIV+EV+LN+SPG SCHC+EPGWFRVCFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 MQRLKAFVLDSTLTTAVIATRNQLDTKRK-SMAKWVFRLSFHDRGCEPRDR 471
           + RLK FV   + T    +   +L   RK  ++ WVFRLSFHDR  EP +R
Sbjct: 421 LDRLKRFVDGPSPTRRSQSEHQRLKNLRKMKVSNWVFRLSFHDR--EPEER 469

BLAST of Tan0000891 vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 699.9 bits (1805), Expect = 1.4e-201
Identity = 332/462 (71.86%), Postives = 394/462 (85.28%), Query Frame = 0

Query: 4   LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLEENP 63
           LS K T N HGQDSSYFLGWEEYEKNPYDEI+NP G+IQMGLAENQLCFDL+E WL +NP
Sbjct: 4   LSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLTKNP 63

Query: 64  DAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGATSA 123
           DAA  KR+G+SIFRELALFQDY+G+P FKKA++EFM EIRGN+VTFDP  IVL AG+TSA
Sbjct: 64  DAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGSTSA 123

Query: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLEAYQ 183
           NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPIHCSSSNGF+ITE+AL +AYQ
Sbjct: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQAYQ 183

Query: 184 NAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRNPGF 243
            AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGT+F    F
Sbjct: 184 QAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGFEQF 243

Query: 244 VSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSSFGL 303
           +S M++L      + ++ K+VH+VYSLSKDLGLPGFRVGAIYSND+++V+AATKMSSFGL
Sbjct: 244 ISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSSFGL 303

Query: 304 VSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFCWVD 363
           VSSQTQYLLSA+LS+KKFT  Y+ ENQKRL+ RQR LV GL+ AGI C + NAGLFCWVD
Sbjct: 304 VSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFCWVD 363

Query: 364 MRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMQR 423
           MRHLL TNTFE+E++LW+KIV+ V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLA++R
Sbjct: 364 MRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLALKR 423

Query: 424 LKAFVLDSTLTTAVIATRNQ--LDTKRKSMAKWVFRLSFHDR 464
           LK FV  +     +  + ++     ++K+++ WVFR+S+ DR
Sbjct: 424 LKTFVESTDCGRMISRSSHERLKSLRKKTVSNWVFRVSWTDR 465

BLAST of Tan0000891 vs. TAIR 10
Match: AT2G22810.1 (1-aminocyclopropane-1-carboxylate synthase 4 )

HSP 1 Score: 680.2 bits (1754), Expect = 1.1e-195
Identity = 332/471 (70.49%), Postives = 392/471 (83.23%), Query Frame = 0

Query: 4   LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLEENP 63
           LSRKATCNSHGQ SSYFLGWEEYEKNPYD  +NP+GIIQMGLAENQLCFDLLE WL +N 
Sbjct: 4   LSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLAQNT 63

Query: 64  DAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGATSA 123
           DAA FKRDG+S+FRELALFQDY+GL +FK A ++FMSE RGN+V+FD N++VLTAGATSA
Sbjct: 64  DAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGATSA 123

Query: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLEAYQ 183
           NETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG+EIVPI  SS+NGF+IT+ AL EAY+
Sbjct: 124 NETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEEAYE 183

Query: 184 NAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIA-SKNLHLISDEIYSGTVFRNPG 243
            A+  +L VKG+L+TNPSNPLGTT +  ELN+L  FI  +KN+HL+SDEIYSGTVF +  
Sbjct: 184 QAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFNSSE 243

Query: 244 FVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSSFG 303
           F+S MEIL  N   N  +  +VHIV SLSKDLGLPGFRVGAIYSND  V++AATKMSSFG
Sbjct: 244 FISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMSSFG 303

Query: 304 LVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFCWV 363
           LVSSQTQYLLS++LS+KKFT+NY+RENQKRL+ RQR LV GL+  GIKC K NAGLFCWV
Sbjct: 304 LVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLFCWV 363

Query: 364 DMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMQ 423
           DMR LLR+ TFE+EM+LW+KIV+EV+LN+SPG SCHC EPGWFRVCFANM + TL LA++
Sbjct: 364 DMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKLALK 423

Query: 424 RLKAFVLDSTLTTAVIATRNQ-LDTKRK----SMAKWVFRLSFHDRGCEPR 469
           RLK  V D   +     ++++ L+  RK    +++ WVFRLSFHDR  E R
Sbjct: 424 RLKMLVDDENSSRRCQKSKSERLNGSRKKTMSNVSNWVFRLSFHDREAEER 474

BLAST of Tan0000891 vs. TAIR 10
Match: AT4G08040.1 (1-aminocyclopropane-1-carboxylate synthase 11 )

HSP 1 Score: 617.8 bits (1592), Expect = 7.0e-177
Identity = 295/458 (64.41%), Postives = 370/458 (80.79%), Query Frame = 0

Query: 3   ILSRKATCNSHGQDSSYFLGWEEYEKNPYDEIQNPKGIIQMGLAENQLCFDLLERWLEEN 62
           +LS K   +SHGQDSSYFLGW+EYEKNP+ E  N  GI+QMGLAENQL FDL+E+WLEE+
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PDAAGFKRDGKSIFRELALFQDYNGLPAFKKALSEFMSEIRGNKVTFDPNHIVLTAGATS 122
           P+  G K++ +S+FR+LALFQDY+GLPAFK A+++FM +IR NKV FD N +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFKITEAALLEAY 182
           ANETLMFCLA PGDAFL+P PYYPGFDRDLKWRTG+EIVPIHC SSNG+KITE AL +AY
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 QNAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFRNPG 242
           + A   NL VKGVL+TNPSNPLGT+ + +EL+LLL F ++K +H++SDEIYSGTVF +P 
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSSFG 302
           F S +E+       N  +  ++H+VYSLSKDLGLPGFRVG IYSN++ VV+AATKMSSFG
Sbjct: 241 FTSVLEVAKDK---NMGLDGKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LVSSQTQYLLSAMLSNKKFTRNYIRENQKRLEKRQRMLVRGLQKAGIKCHKGNAGLFCWV 362
           L+SSQTQ+LL+ +LS+++FT NY+ EN+KRL +R+  LV GL++AGI C K NAGLFCWV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLRTNTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMQ 422
           D+RHLL++NTFE+E  LW KIV EV LN+SPG SCHC EPGWFRVCFANMS+ T+++AM 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RLKAFVLDSTLTTAVIATRNQLDTKRKSMA-KWVFRLS 460
           R+K FV ++         R   DT+R+S+  KWV +LS
Sbjct: 421 RVKGFVDNN---NGGKQKRTMWDTRRRSLINKWVSKLS 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q428815.7e-20875.541-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=... [more]
P378214.5e-20573.251-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 P... [more]
Q002571.0e-20475.811-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN... [more]
Q9M2Y82.9e-20474.511-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9T0651.8e-20171.551-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
XP_022943238.14.3e-25392.901-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata][more]
XP_023522256.13.6e-25292.471-aminocyclopropane-1-carboxylate synthase 3-like [Cucurbita pepo subsp. pepo][more]
KAG6600672.11.4e-25192.471-aminocyclopropane-1-carboxylate synthase 101, partial [Cucurbita argyrosperma ... [more]
XP_022985152.11.8e-25192.471-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita maxima][more]
KAG7031311.18.9e-25191.141-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
A0A6J1FWL02.1e-25392.901-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1J4348.7e-25292.471-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1ENA42.3e-24488.521-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1J4178.2e-24287.271-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0KVS12.2e-23190.16Aminotran_1_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G0992... [more]
Match NameE-valueIdentityDescription
AT3G49700.12.1e-20574.511-aminocyclopropane-1-carboxylate synthase 9 [more]
AT4G37770.11.3e-20271.551-amino-cyclopropane-1-carboxylate synthase 8 [more]
AT5G65800.11.4e-20171.86ACC synthase 5 [more]
AT2G22810.11.1e-19570.491-aminocyclopropane-1-carboxylate synthase 4 [more]
AT4G08040.17.0e-17764.411-aminocyclopropane-1-carboxylate synthase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00753ACCSYNTHASEcoord: 43..69
score: 63.87
coord: 225..248
score: 61.54
coord: 295..318
score: 77.6
coord: 76..92
score: 68.66
coord: 189..213
score: 65.11
coord: 263..287
score: 82.1
coord: 135..156
score: 86.36
coord: 113..133
score: 66.9
NoneNo IPR availablePANTHERPTHR43795BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATEDcoord: 1..437
NoneNo IPR availablePANTHERPTHR43795:SF101-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 9coord: 1..437
NoneNo IPR availableCDDcd00609AAT_likecoord: 77..426
e-value: 1.59968E-64
score: 210.275
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 41..424
e-value: 1.4E-100
score: 337.0
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 29..423
e-value: 3.8E-170
score: 567.8
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 63..318
e-value: 3.8E-170
score: 567.8
IPR004838Aminotransferases, class-I, pyridoxal-phosphate-binding sitePROSITEPS00105AA_TRANSFER_CLASS_1coord: 269..282
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 7..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000891.1Tan0000891.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042218 1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process GO:0009058 biosynthetic process
molecular_function GO:0016847 1-aminocyclopropane-1-carboxylate synthase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity