Tan0000860 (gene) Snake gourd v1

Overview
NameTan0000860
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description(1->3)-beta-glucan endohydrolase
LocationLG08: 2321005 .. 2323721 (+)
RNA-Seq ExpressionTan0000860
SyntenyTan0000860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGGAAGTGCTTCTGGCTCTGTGTTCAATCGCTTCCTGGTTTCTGGGTCGTCTTTTCTGCCGTTGGTACTGTAATTTTTCTCTCTATCTCTATGTTTTCCGTTTCTTCATGTTTTGTAATCAGATGGGGTTTGGGTTTTGAGTTTTGGGTTCTGTTTAGCACTTGGGCAATTTTCTTTTTCATTTCTTTTCTTCTAGATCGATCAGTAGTATTGTATTTTTGTGTTGTTTGTACCATTTTCTCTAAAGATTGCTTCCAGTTGACTAATAGTCTGAAGTTTTCATGGATCTTCCTCCATTTTTCTTTGCTTGATTTAGTCAGCAAGGTTAATTTATTATATTGGACAGTGATTACCAGAATTGTTTATAATATGTTTCTGACTTGCAAGTTTCTAGATTGTTCTTGACTTTGTGCCTGCATCAATTTTTTATTTGATGGGCTATTTTGACTGATTGATTAGTGTGCTTTCAATTGAAAAGAGTTATTTTCTGGGCTAGATTTGAATGCTTCTTTAAGTTCTGTTAAGTGCTTCTCTCATTCTTGAAGATTTTGTTGAGTACTGAGAGTAAATTTGATGTGTGGTTTACAAGTTTGAGGTTTCTTGTAAAGTTATTCACTTGAGGAAGTTTTAGTAAAATGTCTCCTTATCTTTCTCCAGCCTCCTTGACAATGGCAGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCCGATGAAGTTGTTTCGCTTCTCAAAGCGGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTGAAGGCATTTAGTGGGACAGGGCTTGAATTGGTGGTCGGTCTCCCGAATGGTTTCTTGAAAGAGATGAGTGCGAGTGAGGACCATGCAATGAACTGGGTCAAAGAAAATGTCCAGGCATTCCTTCCTCAGACTCACGTTTGTGGGATTGCCATTGGAAATGAAGTTCTGGGAGGAGGTGACTTTGAGTTGTGGGGAGCACTCTTGGGTGCTACAAAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCGTTCAGATCACAACAGCACATTCTCAGGCTGTTTTTTCGAACTCGTTTCCACCTTCTTCGTGTATATTTAGAGAAAACGTTGTGCAGTACATGAAGCCTTTGTTAGAGTTTTTTTCAGAGATCGGTTCACCTTTCTGTTTAAACGCTTACCCATTTCTAGCCTATATGAGTGATCCAGAGAATATTGATATCAACTATGCACTTTTTGAATCAACTGAGGGGATTTTTGATCCTAAAACTAGTCTACATTATGACAACATGCTTGATGCTCAGATTGATGCTGCATATGCAGCATTGGCAGATGCTGGATATCAGAAAATGGAAGTCGTAGTTACAGAGACAGGTTGGGCTTCCCGTGGGGATGAAAACGAAGCTGCAGCAACGGCGAATAATGCTAGGATATTTAATTACAACCTCCGGAAGAGACTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACATTCAACGAAAATTTGAAACCAGGGCCGACTTCTGAGAGAAATTTTGGACTTTTCAAAGCTGATGGAAGTATATCATATGACATTGGATTTCATGGACTTAAATCTTCGTCTTCAGATTCATCTTATTTATCTCTCAAGGTATGTACCACACAGCGTTTGAAGAATGTTATGTTTGTTGAAACGATGATTTTCCCTTCCCGTCCAAATTAACTGATTCAACTAATGACAAAACAAAACGGGTATTCTTGAGCATCATACTTTGTAAGAGTGAAGCTTTTCCCCCCTCTTTTTTGAGTTCAACAATATGTGATTGAGATTCAAACTTCTAACTTTTTATTCGAGGTTATAATGCCTTAACTCTTTAAGCTATACTTAGATTGGTAAGAGAGTGGAGCTTATTGTACTAGATTCCCTTAGAAGCTTACTATACTTGACTTACTTTTGACTTGTTTGAAAGTTTTTTTTCAAGATAATTACCTATTTTTAACAATAAAGATTTAGTAGACACATATTTGAATAACCTTTTTTCAGATAAGGTTTATTTTCAACTACAAAATTGTTTTATAAAAATAGAAAACAAAACAAAGAAAAAGAGGTTATCAAGAAAAGAAACCACTTTATCTTTAAGAAATAGAAAATTAAGTCCCTGTTTGATAACCATGTGGTTTTTCGTTTTTTGTTTTTGAAAATTGCGCTTGTTTTCTCATAATTTCTCTACACTGAGTTTCATCTTCTCTACAATGGTTCCATTTACCTTAAGGAAACATTTGAGTTTCTAGCCGAAATTCAAAAACAAAAACAAGTTTTTGAAAATTACTTTTTTTAGTTTTTAGAACTTGGCTTGGTTTTTGAAAATATATGTAAAAGTGGATAAGAAAACATGGAAACCCATGGGAGGAAGTAGTATTTATATGCTTCATTTTCAAAAACCAAAAACTAAATAGTTATTAAACGGGGCCTAAGTTAATATTGACATCAAAAGCTATTGTATTCTAATCCTACTTCTTTCTTAGTTGGCACTAAACGCAGTCTTTAACGATATTCGTACACATTTGTGAACAGAATCGCCGAGCTCAAGGACTTTTGGCAAGCCACATTTTCTCATTAACAGTCTCCGCCATGACGTTACTTCTATTGTTGAGATGA

mRNA sequence

ATGGCTAGGAAGTGCTTCTGGCTCTGTGTTCAATCGCTTCCTGGTTTCTGGGTCGTCTTTTCTGCCGTTGCCTCCTTGACAATGGCAGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCCGATGAAGTTGTTTCGCTTCTCAAAGCGGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTGAAGGCATTTAGTGGGACAGGGCTTGAATTGGTGGTCGGTCTCCCGAATGGTTTCTTGAAAGAGATGAGTGCGAGTGAGGACCATGCAATGAACTGGGTCAAAGAAAATGTCCAGGCATTCCTTCCTCAGACTCACGTTTGTGGGATTGCCATTGGAAATGAAGTTCTGGGAGGAGGTGACTTTGAGTTGTGGGGAGCACTCTTGGGTGCTACAAAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCGTTCAGATCACAACAGCACATTCTCAGGCTGTTTTTTCGAACTCGTTTCCACCTTCTTCGTGTATATTTAGAGAAAACGTTGTGCAGTACATGAAGCCTTTGTTAGAGTTTTTTTCAGAGATCGGTTCACCTTTCTGTTTAAACGCTTACCCATTTCTAGCCTATATGAGTGATCCAGAGAATATTGATATCAACTATGCACTTTTTGAATCAACTGAGGGGATTTTTGATCCTAAAACTAGTCTACATTATGACAACATGCTTGATGCTCAGATTGATGCTGCATATGCAGCATTGGCAGATGCTGGATATCAGAAAATGGAAGTCGTAGTTACAGAGACAGGTTGGGCTTCCCGTGGGGATGAAAACGAAGCTGCAGCAACGGCGAATAATGCTAGGATATTTAATTACAACCTCCGGAAGAGACTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACATTCAACGAAAATTTGAAACCAGGGCCGACTTCTGAGAGAAATTTTGGACTTTTCAAAGCTGATGGAAGTATATCATATGACATTGGATTTCATGGACTTAAATCTTCGTCTTCAGATTCATCTTATTTATCTCTCAAGAATCGCCGAGCTCAAGGACTTTTGGCAAGCCACATTTTCTCATTAACAGTCTCCGCCATGACGTTACTTCTATTGTTGAGATGA

Coding sequence (CDS)

ATGGCTAGGAAGTGCTTCTGGCTCTGTGTTCAATCGCTTCCTGGTTTCTGGGTCGTCTTTTCTGCCGTTGCCTCCTTGACAATGGCAGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCCGATGAAGTTGTTTCGCTTCTCAAAGCGGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTGAAGGCATTTAGTGGGACAGGGCTTGAATTGGTGGTCGGTCTCCCGAATGGTTTCTTGAAAGAGATGAGTGCGAGTGAGGACCATGCAATGAACTGGGTCAAAGAAAATGTCCAGGCATTCCTTCCTCAGACTCACGTTTGTGGGATTGCCATTGGAAATGAAGTTCTGGGAGGAGGTGACTTTGAGTTGTGGGGAGCACTCTTGGGTGCTACAAAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCGTTCAGATCACAACAGCACATTCTCAGGCTGTTTTTTCGAACTCGTTTCCACCTTCTTCGTGTATATTTAGAGAAAACGTTGTGCAGTACATGAAGCCTTTGTTAGAGTTTTTTTCAGAGATCGGTTCACCTTTCTGTTTAAACGCTTACCCATTTCTAGCCTATATGAGTGATCCAGAGAATATTGATATCAACTATGCACTTTTTGAATCAACTGAGGGGATTTTTGATCCTAAAACTAGTCTACATTATGACAACATGCTTGATGCTCAGATTGATGCTGCATATGCAGCATTGGCAGATGCTGGATATCAGAAAATGGAAGTCGTAGTTACAGAGACAGGTTGGGCTTCCCGTGGGGATGAAAACGAAGCTGCAGCAACGGCGAATAATGCTAGGATATTTAATTACAACCTCCGGAAGAGACTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACATTCAACGAAAATTTGAAACCAGGGCCGACTTCTGAGAGAAATTTTGGACTTTTCAAAGCTGATGGAAGTATATCATATGACATTGGATTTCATGGACTTAAATCTTCGTCTTCAGATTCATCTTATTTATCTCTCAAGAATCGCCGAGCTCAAGGACTTTTGGCAAGCCACATTTTCTCATTAACAGTCTCCGCCATGACGTTACTTCTATTGTTGAGATGA

Protein sequence

MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLKSSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR
Homology
BLAST of Tan0000860 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 5.0e-88
Identity = 157/335 (46.87%), Postives = 226/335 (67.46%), Query Frame = 0

Query: 25  SLTMAVQAFTGT-YGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFSGTG 84
           SLT+ +   T T  G+NYG+I DN+PSP +V+ L+K+     V++YDA+ ++LKAFS TG
Sbjct: 16  SLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNTG 75

Query: 85  LELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGAL 144
           +E ++GL N +L +M      A+ W+K+NV  FLP T++  I IGNE+L   D  L   L
Sbjct: 76  IEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTNL 135

Query: 145 LGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEI 204
           L A + ++ A+    L   + +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  + 
Sbjct: 136 LPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRKT 195

Query: 205 GSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALAD 264
            SPF +NAYPF AY  +P+ + +++ LF+  +GI DP T  HYDNML AQIDA Y+ALA 
Sbjct: 196 DSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALAA 255

Query: 265 AGYQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRKRL--AKKKGTPFRPKNVLKA 324
           AG++ + V ++ETGW S+GD++E  AT  NA+ +N NL K +   KK  TP +P N L  
Sbjct: 256 AGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLSI 315

Query: 325 YIFATFNENLKPGPTSERNFGLFKADGSISYDIGF 357
           Y+FA FNENLKPGPTSERN+GLFK DG+ +Y +GF
Sbjct: 316 YVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

BLAST of Tan0000860 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 318.2 bits (814), Expect = 1.4e-85
Identity = 147/321 (45.79%), Postives = 216/321 (67.29%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD  VL +FS + ++ ++GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  MSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKL 157
           MS     A +W+++ ++  + +T +  I +GNE+    D  L  +LL A K++Y A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
            L+  V +T+AHS  + S S+PPSS  F+E  +QY++PLL+F S+I SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETG 277
              P+ + + Y LF+  +G+ DP T+LHYDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           W S+GDENE  A+  NA ++N NL K + ++KGTP +    +  Y+FA FNENLKPGP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 338 ERNFGLFKADGSISYDIGFHG 359
           ERN+GLF  DG   Y++G  G
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

BLAST of Tan0000860 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.4e-82
Identity = 158/352 (44.89%), Postives = 227/352 (64.49%), Query Frame = 0

Query: 19  VFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKA 78
           +FS       ++     + GINYG++A+N+P P  V+ LLK+     V++YDAD + L+A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 79  FSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFE 138
           F+G+G EL V L N +L +MS     A  WVKENVQA+LP T +  I +GNEVL      
Sbjct: 69  FAGSGFELTVALGNEYLAQMS-DPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 128

Query: 139 LWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLE 198
           L  AL  A ++I+ A+    L+  + +TTAHS A+   S+PPS+  FR +++  + P+L+
Sbjct: 129 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 188

Query: 199 FFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAY 258
           F  + GSP  +NAYPF AY  +P+++ +++ LF+  +G  DP ++ HYDNML AQ+DA Y
Sbjct: 189 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 248

Query: 259 AALADAG--YQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRK-RLAKKKGTPFRP 318
            AL   G  Y+K+ +VV+ETGW S GD  E  AT +NAR +N NL K  ++KK  TP RP
Sbjct: 249 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 308

Query: 319 KNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLKSSSSDSS 368
           +  L  ++FA FNEN+KPGPTSERN+GLF  DG+  Y +   G+K+SS+ SS
Sbjct: 309 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSL---GIKTSSTHSS 356

BLAST of Tan0000860 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 1.0e-80
Identity = 148/330 (44.85%), Postives = 210/330 (63.64%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKE 97
           G+NYG++ADN+P P E V LL++  I+ VR+Y AD  ++KA +GTG+ +V+G  NG +  
Sbjct: 27  GVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPS 86

Query: 98  MSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKL 157
           +++  + A  W+  NV  F P + +  I +GNE+L   D  L   LL A +N+ KA++ +
Sbjct: 87  LASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAV 146

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
           +L G ++++T +S  V  +S PPSS  F       +K +L+F S+ GSPF +N YPF AY
Sbjct: 147 SLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAY 206

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETG 277
            SDP    + + LFE   G  D KT + Y NM DAQ+DA ++AL   G++K+E+VV ETG
Sbjct: 207 QSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETG 266

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           WASRGD NE  A+ +NA+ +N NL   L    GTP  P   +  YIFA ++ENLKPGP+S
Sbjct: 267 WASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSS 326

Query: 338 ERNFGLFKADGSISYDIGFHGLKSSSSDSS 368
           ER FGLFK D S+ YD+G    KSSSS  +
Sbjct: 327 ERAFGLFKTDLSMVYDVGL--AKSSSSSQT 354

BLAST of Tan0000860 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.7e-75
Identity = 146/343 (42.57%), Postives = 209/343 (60.93%), Query Frame = 0

Query: 16  FWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKV 75
           FW+  S +A L   +       GI YGR ADN+PSP+ V  L++   IK VRIYDA+  V
Sbjct: 9   FWIFVSILAFLNFGM---ASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDV 68

Query: 76  LKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGG 135
           LKAF+ TG+EL++G+PN  L   +  + +   W+  N+  + P T +  I++G EV    
Sbjct: 69  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 128

Query: 136 DFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKP 195
           D    G +L A +NI+ A+KK  LD  ++I+++HS A+ S SFPPSS  F +    ++KP
Sbjct: 129 D-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKP 188

Query: 196 LLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQID 255
           +LEF  E  SPF ++ YP+ AY    E + + YALFES+  + DP T L Y NM DAQ+D
Sbjct: 189 MLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLD 248

Query: 256 AAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRP 315
           A Y AL    ++ ++V+VTE+GW S+G   E AAT  NA  +N NL + +    GTP +P
Sbjct: 249 AIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKP 308

Query: 316 KNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHG 359
              +  Y+F+ FNEN KPG  SERN+G+F A+G+  Y + F G
Sbjct: 309 GEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTG 347

BLAST of Tan0000860 vs. NCBI nr
Match: XP_008457422.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo])

HSP 1 Score: 749.2 bits (1933), Expect = 1.8e-212
Identity = 371/399 (92.98%), Postives = 385/399 (96.49%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C QS+PGFWVVFSA+ASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFL+EMSASE+HAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGA KNIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEV+VTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSSDSSYLSLK+ RA+GL  SH+ SLT+SA+TLLLLLR
Sbjct: 361 SSSSDSSYLSLKDFRARGLSGSHMISLTISAVTLLLLLR 399

BLAST of Tan0000860 vs. NCBI nr
Match: XP_038889136.1 (glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida])

HSP 1 Score: 746.1 bits (1925), Expect = 1.5e-211
Identity = 368/399 (92.23%), Postives = 383/399 (95.99%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C QSLPGFWVVFSA+ASLTM VQAFTGTYGINYGRIADNIPSPDEVV LLKA
Sbjct: 1   MARKCFWFCFQSLPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVLLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAFSGTGLELV+GLPNGFL+EMSASE+HAMNWVKENVQAFLP T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVIGLPNGFLREMSASEEHAMNWVKENVQAFLPDT 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGG D ELWGALLGA KNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGSDLELWGALLGAAKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLL+F SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLQFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEV+VTETGWAS GDENEAAATANNARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASHGDENEAAATANNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLF+ADGSISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFQADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           + SSDSSYLSLKN RA+GL A+H+FS  +SA+TLLLLLR
Sbjct: 361 APSSDSSYLSLKNFRARGLWANHVFSFMISAVTLLLLLR 399

BLAST of Tan0000860 vs. NCBI nr
Match: XP_004139302.2 (glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN60690.1 hypothetical protein Csa_019301 [Cucumis sativus])

HSP 1 Score: 745.7 bits (1924), Expect = 2.0e-211
Identity = 370/399 (92.73%), Postives = 383/399 (95.99%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C QSL GFWVVFSA+ASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSLSGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFL+EMSASE+HAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWG LLGA KNIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGTLLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEV+VTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
            SSSDSSYLSLK+ RA+GLL SH+ SLT++AMTLLLLLR
Sbjct: 361 YSSSDSSYLSLKDFRARGLLGSHMISLTITAMTLLLLLR 399

BLAST of Tan0000860 vs. NCBI nr
Match: XP_022938105.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata])

HSP 1 Score: 742.7 bits (1916), Expect = 1.7e-210
Identity = 370/399 (92.73%), Postives = 383/399 (95.99%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK FW C QS+PGFWVVFSA+ASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELV+GLPNGFLKEMS+SE+ AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEV+VTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSS SSYLSLKN RA+GL  S IFSLTVS +T LLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVVTRLLLLR 399

BLAST of Tan0000860 vs. NCBI nr
Match: KAG6586386.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741.1 bits (1912), Expect = 5.0e-210
Identity = 369/399 (92.48%), Postives = 383/399 (95.99%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK FW C QS+PGFWVVFSA+ASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELV+GLPNGFLKEMS+SE+ AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYM+PLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMEPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEV+VTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSS SSYLSLKN RA+GL  S IFSLTVS +T LLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVVTRLLLLR 399

BLAST of Tan0000860 vs. ExPASy TrEMBL
Match: A0A1S3C5G6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.8e-213
Identity = 371/399 (92.98%), Postives = 385/399 (96.49%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C QS+PGFWVVFSA+ASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFL+EMSASE+HAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGA KNIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEV+VTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSSDSSYLSLK+ RA+GL  SH+ SLT+SA+TLLLLLR
Sbjct: 361 SSSSDSSYLSLKDFRARGLSGSHMISLTISAVTLLLLLR 399

BLAST of Tan0000860 vs. ExPASy TrEMBL
Match: A0A0A0LF78 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.8e-212
Identity = 370/399 (92.73%), Postives = 383/399 (95.99%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFW C QSL GFWVVFSA+ASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSLSGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFL+EMSASE+HAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWG LLGA KNIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGTLLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEV+VTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
            SSSDSSYLSLK+ RA+GLL SH+ SLT++AMTLLLLLR
Sbjct: 361 YSSSDSSYLSLKDFRARGLLGSHMISLTITAMTLLLLLR 399

BLAST of Tan0000860 vs. ExPASy TrEMBL
Match: A0A6J1FIR4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444294 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 8.3e-211
Identity = 370/399 (92.73%), Postives = 383/399 (95.99%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK FW C QS+PGFWVVFSA+ASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELV+GLPNGFLKEMS+SE+ AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEV+VTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSS SSYLSLKN RA+GL  S IFSLTVS +T LLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVVTRLLLLR 399

BLAST of Tan0000860 vs. ExPASy TrEMBL
Match: A0A6J1KA33 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493627 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.1e-210
Identity = 365/399 (91.48%), Postives = 384/399 (96.24%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKCFWLCVQSLPGFW+VFSA+AS TM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWLCVQSLPGFWIVFSAIASSTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADH VLKAFSGTGLEL VGLPNGFLKE SASE+HAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHNVLKAFSGTGLELEVGLPNGFLKETSASEEHAMNWVKENVQAFLPET 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGA KNIYKAVK LNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKSLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYM+PLLEF +EIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GI DP
Sbjct: 181 SSCIFRENVVQYMRPLLEFLAEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIIDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEV+VTETGWASRGDENEAAAT +NA+IFNYN
Sbjct: 241 KTDLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATVSNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTP+RP+NVLKAY+FATFNENLK GPTSERNFGLFKADGSISYD+GFHGLK
Sbjct: 301 LRKRLAKKKGTPYRPRNVLKAYVFATFNENLKQGPTSERNFGLFKADGSISYDVGFHGLK 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSSDSSYLSLK+ RA+GLLA+HIFSLT SA+TLLLLLR
Sbjct: 361 SSSSDSSYLSLKSFRARGLLANHIFSLTFSAVTLLLLLR 399

BLAST of Tan0000860 vs. ExPASy TrEMBL
Match: A0A6J1HML7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465570 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.6e-209
Identity = 369/399 (92.48%), Postives = 381/399 (95.49%), Query Frame = 0

Query: 1   MARKCFWLCVQSLPGFWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  W C QS+PGFWVVFSA+ASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELVVGLPNGFLKEMS+SE+ AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           H+CGIAIGNEVLGGGD ELWGALLGATKNIYKAVKKLNLDGV+QITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEI SPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEV+VTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLLLR 400
           SSSS SSYLSLKN RA+GL  S IFSLTVS MT LLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 399

BLAST of Tan0000860 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.2e-153
Identity = 271/384 (70.57%), Postives = 316/384 (82.29%), Query Frame = 0

Query: 16  FWVVFSAVASLTMAVQ-AFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHK 75
           F+  F  + SL   V  AF GTYGINYGRIADNIPSP++VV LLK AKI+NVRIYD DH 
Sbjct: 12  FFFFFFNLTSLEYQVDGAFVGTYGINYGRIADNIPSPEKVVLLLKQAKIRNVRIYDVDHT 71

Query: 76  VLKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGG 135
           VL+AFSGTGL+LVVGLPNGFLKEMS++ DHA +WVKEN+Q+FLP+T + GIAIGNEVLGG
Sbjct: 72  VLEAFSGTGLDLVVGLPNGFLKEMSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGG 131

Query: 136 GDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMK 195
           GD EL GALLGA KN+Y A+KK+NL+  VQITTAHSQAVFS+S+PPSSC+F+ENVVQ+MK
Sbjct: 132 GDSELAGALLGAAKNVYNALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMK 191

Query: 196 PLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQI 255
           PLLEFF +IGSPFCLNAYPFLAY  +P+ IDINYALF+ TEGI+DPKT LHYDNMLDAQI
Sbjct: 192 PLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQI 251

Query: 256 DAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFR 315
           DAAY AL DAG++KMEV++TETGWAS+GD +E AAT  NAR +NYNLRKRLAKKKGTP R
Sbjct: 252 DAAYMALQDAGFKKMEVMITETGWASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLR 311

Query: 316 PKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLKSSSSDSSYLSLKNR 375
           PK VLKAYIFA FNEN KPG +SE +FGLFK DG+ISYDIGF+ LKS S  S   S K+ 
Sbjct: 312 PKTVLKAYIFALFNENSKPGKSSETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSKSA 371

Query: 376 RAQGLLASHIFSLTVSAMTLLLLL 399
           R       +  +L +S    LL++
Sbjct: 372 R-------YYVALVISVSAFLLMI 388

BLAST of Tan0000860 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 469.9 bits (1208), Expect = 2.0e-132
Identity = 226/350 (64.57%), Postives = 279/350 (79.71%), Query Frame = 0

Query: 16  FWVVFSAVASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKV 75
           F +VFS ++S T    AF GTYG+NYGRIADN+PSPD V +LLK+AKI+N RIYDADH V
Sbjct: 24  FLLVFSILSSQTAV--AFIGTYGVNYGRIADNLPSPDAVATLLKSAKIRNTRIYDADHSV 83

Query: 76  LKAFSGTGLELVVGLPNGFLKEMSASEDHAMNWVKENVQAFL-PQTHVCGIAIGNEVLGG 135
           L AF GTG+E++VGL N FLK++S  ED AMNW+KENV+ F+   T + GIA+GNE+LGG
Sbjct: 84  LTAFRGTGIEIIVGLGNEFLKDISVGEDRAMNWIKENVEPFIRGGTKISGIAVGNEILGG 143

Query: 136 GDFELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMK 195
            D  LW ALL A KN+Y A+++L L  VV++++ HS+AVF+NS+PPSSC FR++V  +MK
Sbjct: 144 TDIGLWEALLPAAKNVYSALRRLGLHNVVEVSSPHSEAVFANSYPPSSCTFRDDVAPFMK 203

Query: 196 PLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQI 255
           PLL FF +I SPF +NAYPFLAY SDP  IDINYALFE  +GI DPKT LHYDNM DA +
Sbjct: 204 PLLAFFWQIQSPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDNMFDAMV 263

Query: 256 DAAYAALADAGYQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFR 315
           DA+YAAL  AGY K+ V+V+ETGWAS+GD +E  A+  NAR +N NLRKRL K+KGTP+R
Sbjct: 264 DASYAALEKAGYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKRKGTPYR 323

Query: 316 PKNVLKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLKSSSS 365
           P  V++AY+FA FNEN KPGPTSERNFGLFK DG+I+YDIG  GLKSSS+
Sbjct: 324 PDMVVRAYVFALFNENSKPGPTSERNFGLFKPDGTIAYDIGLTGLKSSSA 371

BLAST of Tan0000860 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 4.3e-119
Identity = 210/301 (69.77%), Postives = 248/301 (82.39%), Query Frame = 0

Query: 98  MSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKL 157
           MS++ DHA +WVKEN+Q+FLP+T + GIAIGNEVLGGGD EL GALLGA KN+Y A+KK+
Sbjct: 1   MSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVYNALKKM 60

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
           NL+  VQITTAHSQAVFS+S+PPSSC+F+ENVVQ+MKPLLEFF +IGSPFCLNAYPFLAY
Sbjct: 61  NLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAY 120

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETG 277
             +P+ IDINYALF+ TEGI+DPKT LHYDNMLDAQIDAAY AL DAG++KMEV++TETG
Sbjct: 121 TYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEVMITETG 180

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           WAS+GD +E AAT  NAR +NYNLRKRLAKKKGTP RPK VLKAYIFA FNEN KPG +S
Sbjct: 181 WASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENSKPGKSS 240

Query: 338 ERNFGLFKADGSISYDIGFHGLKSSSSDSSYLSLKNRRAQGLLASHIFSLTVSAMTLLLL 397
           E +FGLFK DG+ISYDIGF+ LKS S  S   S K+ R       +  +L +S    LL+
Sbjct: 241 ETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSKSAR-------YYVALVISVSAFLLM 294

Query: 398 L 399
           +
Sbjct: 301 I 294

BLAST of Tan0000860 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 3.6e-89
Identity = 157/335 (46.87%), Postives = 226/335 (67.46%), Query Frame = 0

Query: 25  SLTMAVQAFTGT-YGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFSGTG 84
           SLT+ +   T T  G+NYG+I DN+PSP +V+ L+K+     V++YDA+ ++LKAFS TG
Sbjct: 16  SLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNTG 75

Query: 85  LELVVGLPNGFLKEMSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGAL 144
           +E ++GL N +L +M      A+ W+K+NV  FLP T++  I IGNE+L   D  L   L
Sbjct: 76  IEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTNL 135

Query: 145 LGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEI 204
           L A + ++ A+    L   + +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  + 
Sbjct: 136 LPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRKT 195

Query: 205 GSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALAD 264
            SPF +NAYPF AY  +P+ + +++ LF+  +GI DP T  HYDNML AQIDA Y+ALA 
Sbjct: 196 DSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALAA 255

Query: 265 AGYQKMEVVVTETGWASRGDENEAAATANNARIFNYNLRKRL--AKKKGTPFRPKNVLKA 324
           AG++ + V ++ETGW S+GD++E  AT  NA+ +N NL K +   KK  TP +P N L  
Sbjct: 256 AGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLSI 315

Query: 325 YIFATFNENLKPGPTSERNFGLFKADGSISYDIGF 357
           Y+FA FNENLKPGPTSERN+GLFK DG+ +Y +GF
Sbjct: 316 YVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

BLAST of Tan0000860 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 9.7e-87
Identity = 147/321 (45.79%), Postives = 216/321 (67.29%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD  VL +FS + ++ ++GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  MSASEDHAMNWVKENVQAFLPQTHVCGIAIGNEVLGGGDFELWGALLGATKNIYKAVKKL 157
           MS     A +W+++ ++  + +T +  I +GNE+    D  L  +LL A K++Y A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
            L+  V +T+AHS  + S S+PPSS  F+E  +QY++PLL+F S+I SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVVVTETG 277
              P+ + + Y LF+  +G+ DP T+LHYDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           W S+GDENE  A+  NA ++N NL K + ++KGTP +    +  Y+FA FNENLKPGP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 338 ERNFGLFKADGSISYDIGFHG 359
           ERN+GLF  DG   Y++G  G
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8685.0e-8846.87Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Q9ZQG91.4e-8545.79Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500... [more]
Q9FHX51.4e-8244.89Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100... [more]
Q9M0691.0e-8044.85Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE53.7e-7542.57Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
XP_008457422.11.8e-21292.98PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo][more]
XP_038889136.11.5e-21192.23glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida][more]
XP_004139302.22.0e-21192.73glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN60690.1 hypothetical p... [more]
XP_022938105.11.7e-21092.73glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata][more]
KAG6586386.15.0e-21092.48Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
A0A1S3C5G68.8e-21392.98(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV... [more]
A0A0A0LF789.8e-21292.73(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3... [more]
A0A6J1FIR48.3e-21192.73(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444294 P... [more]
A0A6J1KA333.1e-21091.48(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493627 PE=... [more]
A0A6J1HML71.6e-20992.48(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465570 PE=... [more]
Match NameE-valueIdentityDescription
AT2G26600.11.2e-15370.57Glycosyl hydrolase superfamily protein [more]
AT3G15800.12.0e-13264.57Glycosyl hydrolase superfamily protein [more]
AT2G26600.24.3e-11969.77Glycosyl hydrolase superfamily protein [more]
AT1G32860.13.6e-8946.87Glycosyl hydrolase superfamily protein [more]
AT2G27500.19.7e-8745.79Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 38..356
e-value: 1.6E-78
score: 264.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 36..362
e-value: 7.8E-115
score: 385.2
NoneNo IPR availablePANTHERPTHR32227:SF129GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 27..390
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 27..390
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 37..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000860.1Tan0000860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds