Tan0000821 (gene) Snake gourd v1

Overview
NameTan0000821
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRING-type E3 ubiquitin transferase
LocationLG07: 10413666 .. 10416833 (-)
RNA-Seq ExpressionTan0000821
SyntenyTan0000821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCTTCTTGGCCTCTGCAAAAGCCATTACTGACTGTGTCACTAATAAAGACGAAGCCAAGCGCAGCCCCATTTCAAAAAGGTTTCTAAAATATATTCCCCTTCCTCACAAAATCCTCGCAAGGTGTCGCACCGCGGTCTCACGAGCTCGTTTCTTTGTTCTTTTCCTCCATTTTCTTTATTTTTAATTCTCTGTTCCAATTGAACCAGATGATTTCTACGAAAGAATCAATCACAATCACATTCACCAGGCGATCGAGCAGCAACCAAATTTGTTGACTCTCGAGCGTTTTGTTGTGCCAATCAAAGGGTGACACAAGTTGAACCCTCGCCCAACGCTCGCGGGCATTGCTCCACCTCACCACCCATCCCCTTCGTCTTGTCCTTGATTTCCTGTTGGGGCCCAAGATTTTATGCAATTATTGGATTGTTGAGCGGCGTGAAGTTGAATATTCAACCATTTGGGGTTCAGAATCTTTTCTCCCTACGGCATTTCTTTACCATTTTGCAACTTCTTCTGCTGTTTTCTGGTCGATCAACTTCAGTACCTTCAATTTTTCCATCTGGGTACGGAGGTTTCTTTGCCTACTTTTAATGTTTCTTCGTCATTGCGTTGCTCTGCTGTTGATTCTGTGAAAATCTCTCTTGGGGTTTGTTTAATTTCGTAGTTGTCGTATGTTTTCAACATTGTTTATTAGTGTGCTGCATTCTGGTTATGCTCGTGGCTGTTAAACAATTCGAAACCATGGCGAATTTGTCTGTCTTTTTCCTTCTTGATTGTTAACTGAAGTGGTTTAGATGGAAAAATGCTGGCTTCTGTCAATCAGTTTTAAACAGAGGTGATCCGTTCTATTAATAGATAAATTGTTGTTTACTTTTTAGCTTCCGTATATTGTACTTTTCTGTTTATAGATTCTTATCCCAACGCTGATGCCAAACTCAAAAAAGAGGGAAAAGGAAAAAAAATTTTGGTGCTTCTGTTGGGTTTTGTTTTCTTGCTTGTTGTACTTGTGCCAATAATGATACAACTTTTGTTTCTCACTCTTAAACGCACTTTTCTGTTGTACAAGCAACCAATGATTCCATCCCGTTATGACTGCCATGTTGTTGTAAATTTTAATGCAAACATGGATTTTCGATTAAAAGAACCTTGAAGATTCTTCCTCATAATTTCCATTTCCAACAGGTTCCGTCCTAATCCTTCCTATGCTATTGTAAGATATGCCTTTCTTTCATGATGTATTTATGGAAGCATTGAATGATTTGAAACCTAATTGTCTTTACATCTTGCATATGTAGTGCTTAAAAATTTCATCCTACTTCTTCTCTTGATTTGTCGATATAGATTTGATTTAGGTATGTAATAACTATACGCCGTAATACGGCTTTATAGATAGATTGTTCTTGATTTGCAATTTCCATAGATTCATGTTTTGAACCTTCTTTCTATTATTATGCAATTTGTATTGCACCTTAAAAATAAATATCTGGTTAAGTTTGAATAGACTCCACATTGCTGACATGATTCCTTCAAAATTGATTGGTCTGTGGAACCAGTTTTGAGATGCTTGCATAACTTAAGTTTCAATAGAACCAGTTATGAGTTCTTCAAAGAAGTCCCTCCAGCAATGACACTTCTTTTTTTTTTTTAACTAAATCTCAGAGATGATTGCATGAGTACTGTTGAATTCAGAGAGAAGGGGATGTATCAGCAGCAGAAGTCAAGGGAAGAACGTGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTGCATCTATTGATTCTCAAGCAGCAACTTCTCGTCCAGAGTGCAGAGGTGTCTCTTTCAGGAAAGCTTCTGCTGGTGGTGGTTTTGCCAGGAATCGAAATGATGTACACGAGCTACTCGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGGTATCATATTCTATAATTGTGATGAGTTTCAAAACTCACGGTGGTTGCTCATAAATTATTCTGGAAATACATTGCCGTAAATATATCCATCCAATAAGTAGAAAAAAAAGCATTTAATTTGAAACCCTGAACCAAAGTTAATGTCTAGAAGCCTCGTGCATTATTGAGCAAATGGAAGGCTATTTTGTTTTACCATTGTCTTGAGTTGTGGCTGAGTTTTTACATTCGAGGCAACTTTAATGCATATCAGCTAATATTAGTTTATGCTGTAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAACTGCTGCCCTACTTGTCGCCACGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTCTGTACGAAAACCTAGGTTGCGTTGACATGTTCCCATACTACAGCAAGCTCAAACATGAGAAGAACTGTAAATACCGCCCGTACAACTGCCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCATACCTTGTCATGCATCTCAAGGACGATCACGAGGTCGATATGCATGATGGGTGTTCCTTCAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTAAGATTTCTCTCTGTCCTCTGTGTGTTTATGGATGGAGTCTCTTGCACAAACTGATTCTTAGGAACAAAAGCAAAAAAGCATTTCATGTAGCATCCTCTGATTTTCATTCAATCCAATGCAGGTTTTTAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCCGTATACATGGCGTTCCTCCGGTTCATGGGCGACGATGACGACGCAAAACAATTTTCTTACAGTCTGGAAGTTGGTGGAAATGGCAGAAAACTCATCTGGCAAGGTATGCCGAGAAGTATCCGCGATAGCCATCGGAAGGTTCGGGACAGTCACGACGGATTAATCATTCAGAGGAAGTTGGCACTATTCTGCTCTGGAAATGAGGGGCAGGAACTCAAGCTAAAGGTTGCAGGGCGAATATGGAGAGTGAGGTGAGAAGAAATATACATTCTCAAAGTCAGAAGTTGTACAATATGTGACAGTGACCTCTGTAAATTGGCTGCTACAATTCAGCCATTGTTAATTTGTTAGAAGCTGATATTTCATCCCCTAAA

mRNA sequence

CAGCTTCTTGGCCTCTGCAAAAGCCATTACTGACTGTGTCACTAATAAAGACGAAGCCAAGCGCAGCCCCATTTCAAAAAGGTTTCTAAAATATATTCCCCTTCCTCACAAAATCCTCGCAAGGTGTCGCACCGCGGTCTCACGAGCTCGTTTCTTTGTTCTTTTCCTCCATTTTCTTTATTTTTAATTCTCTGTTCCAATTGAACCAGATGATTTCTACGAAAGAATCAATCACAATCACATTCACCAGGCGATCGAGCAGCAACCAAATTTGTTGACTCTCGAGCGTTTTGTTGTGCCAATCAAAGGGTGACACAAGTTGAACCCTCGCCCAACGCTCGCGGGCATTGCTCCACCTCACCACCCATCCCCTTCGTCTTGTCCTTGATTTCCTGTTGGGGCCCAAGATTTTATGCAATTATTGGATTGTTGAGCGGCGTGAAGTTGAATATTCAACCATTTGGGGTTCAGAATCTTTTCTCCCTACGGCATTTCTTTACCATTTTGCAACTTCTTCTGCTGTTTTCTGGTCGATCAACTTCAGTACCTTCAATTTTTCCATCTGGTGTGCTGCATTCTGGTTATGCTCGTGGCTGTTAAACAATTCGAAACCATGGCGAATTTGTCTGTCTTTTTCCTTCTTGATTGTTAACTGAAGTGGTTTAGATGGAAAAATGCTGGCTTCTGTCAATCAGTTTTAAACAGAGGTGATCCGTTCTATTAATAGATAAATTGTTGTTTACTTTTTAGCTTCCGTATATTGTACTTTTCTGTTTATAGATTCTTATCCCAACGCTGATGCCAAACTCAAAAAAGAGGGAAAAGGAAAAAAAATTTTGGTGCTTCTGTTGGGTTTTGTTTTCTTGCTTGTTGTACTTGTGCCAATAATGATACAACTTTTGTTTCTCACTCTTAAACGCACTTTTCTGTTGTACAAGCAACCAATGATTCCATCCCGTTATGACTGCCATGTTGTTGTAAATTTTAATGCAAACATGGATTTTCGATTAAAAGAACCTTGAAGATTCTTCCTCATAATTTCCATTTCCAACAGGTTCCGTCCTAATCCTTCCTATGCTATTGTAAGATATGCCTTTCTTTCATGATGTATTTATGGAAGCATTGAATGATTTGAAACCTAATTGTCTTTACATCTTGCATATGTAGTGCTTAAAAATTTCATCCTACTTCTTCTCTTGATTTGTCGATATAGATTTGATTTAGAGATGATTGCATGAGTACTGTTGAATTCAGAGAGAAGGGGATGTATCAGCAGCAGAAGTCAAGGGAAGAACGTGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTGCATCTATTGATTCTCAAGCAGCAACTTCTCGTCCAGAGTGCAGAGGTGTCTCTTTCAGGAAAGCTTCTGCTGGTGGTGGTTTTGCCAGGAATCGAAATGATGTACACGAGCTACTCGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAACTGCTGCCCTACTTGTCGCCACGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTCTGTACGAAAACCTAGGTTGCGTTGACATGTTCCCATACTACAGCAAGCTCAAACATGAGAAGAACTGTAAATACCGCCCGTACAACTGCCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCATACCTTGTCATGCATCTCAAGGACGATCACGAGGTCGATATGCATGATGGGTGTTCCTTCAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTTTTTAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCCGTATACATGGCGTTCCTCCGGTTCATGGGCGACGATGACGACGCAAAACAATTTTCTTACAGTCTGGAAGTTGGTGGAAATGGCAGAAAACTCATCTGGCAAGGTATGCCGAGAAGTATCCGCGATAGCCATCGGAAGGTTCGGGACAGTCACGACGGATTAATCATTCAGAGGAAGTTGGCACTATTCTGCTCTGGAAATGAGGGGCAGGAACTCAAGCTAAAGGTTGCAGGGCGAATATGGAGAGTGAGGTGAGAAGAAATATACATTCTCAAAGTCAGAAGTTGTACAATATGTGACAGTGACCTCTGTAAATTGGCTGCTACAATTCAGCCATTGTTAATTTGTTAGAAGCTGATATTTCATCCCCTAAA

Coding sequence (CDS)

ATGAGTACTGTTGAATTCAGAGAGAAGGGGATGTATCAGCAGCAGAAGTCAAGGGAAGAACGTGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTGCATCTATTGATTCTCAAGCAGCAACTTCTCGTCCAGAGTGCAGAGGTGTCTCTTTCAGGAAAGCTTCTGCTGGTGGTGGTTTTGCCAGGAATCGAAATGATGTACACGAGCTACTCGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAACTGCTGCCCTACTTGTCGCCACGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCGCTCGAGCTCCCTTGTCTGTACGAAAACCTAGGTTGCGTTGACATGTTCCCATACTACAGCAAGCTCAAACATGAGAAGAACTGTAAATACCGCCCGTACAACTGCCCTTATGCTGGAGGTGAATGTTCTGTAACTGGTGACATTCCATACCTTGTCATGCATCTCAAGGACGATCACGAGGTCGATATGCATGATGGGTGTTCCTTCAGCCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCTACATGGATGCTTACTGTTTTTAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCTTAGGAACAGCACCCGTATACATGGCGTTCCTCCGGTTCATGGGCGACGATGACGACGCAAAACAATTTTCTTACAGTCTGGAAGTTGGTGGAAATGGCAGAAAACTCATCTGGCAAGGTATGCCGAGAAGTATCCGCGATAGCCATCGGAAGGTTCGGGACAGTCACGACGGATTAATCATTCAGAGGAAGTTGGCACTATTCTGCTCTGGAAATGAGGGGCAGGAACTCAAGCTAAAGGTTGCAGGGCGAATATGGAGAGTGAGGTGA

Protein sequence

MSTVEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKASAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGQELKLKVAGRIWRVR
Homology
BLAST of Tan0000821 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.5e-130
Identity = 218/301 (72.43%), Postives = 251/301 (83.39%), Query Frame = 0

Query: 27  SFCKEVTELRLASIDSQAATSRPECRGVSFRK-ASAGGG--FARNRNDVHELLDCTVCMN 86
           S  KEV E     +D +  T++ E       K  SAG G     + N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 87  LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCLYENLGC 146
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 147 VDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGCSFSHR 206
            D+FPYYSKLKHE++C++RPY CPYAG ECSVTGDIP LV+HLKDDH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 207 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 266
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 267 LEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGQELKLKVAGRIW 325
           LEVG +GRKL WQG+PRSIRDSHRKVRDS DGLII R LAL+ SG + QELKL+V GRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

BLAST of Tan0000821 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 1.6e-129
Identity = 211/298 (70.81%), Postives = 246/298 (82.55%), Query Frame = 0

Query: 27  SFCKEVTELRLASIDSQAATSRPECRGVSFRKASAGGGFARNRNDVHELLDCTVCMNLMH 86
           S  KE  E     +D +   ++ E      +  S   G   + N V+ELL+C VC NLM+
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMY 65

Query: 87  PPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCLYENLGCVDM 146
           PPI+QC NGHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC D+
Sbjct: 66  PPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDI 125

Query: 147 FPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGCSFSHRYVK 206
           FPYYSKLKHE++C++R Y+CPYAG ECSVTGDIP LV HLKDDH+VDMHDGC+F+HRYVK
Sbjct: 126 FPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVK 185

Query: 207 SNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEV 266
           SNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYSLEV
Sbjct: 186 SNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEV 245

Query: 267 GGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGQELKLKVAGRIWR 325
           G + RKL WQG+PRSIRDSHRKVRDS DGLII R LAL+ SG++ +ELKL+V GRIW+
Sbjct: 246 GAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

BLAST of Tan0000821 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 1.6e-124
Identity = 196/255 (76.86%), Postives = 226/255 (88.63%), Query Frame = 0

Query: 70  NDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVA 129
           + V ELL+C VC++ M+PPI+QC+NGHTLCS CK RV N CPTCRHELGNIRCLALEKVA
Sbjct: 45  SSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 104

Query: 130 ESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDD 189
            SLELPC Y+N GCV ++PYY KLKHE  C+YRPY+CPYAG EC+V GDIPYLV HLKDD
Sbjct: 105 ASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDD 164

Query: 190 HEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLR 249
           H+VDMH+GC+F+HRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAF+LG APVY+AFLR
Sbjct: 165 HKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 224

Query: 250 FMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGN 309
           FMGDD +AK +SYSLEVGG GRK+IWQG+PRSIRDSHRKVRDS+DGLIIQR +ALF SG 
Sbjct: 225 FMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGG 284

Query: 310 EGQELKLKVAGRIWR 325
           E +ELKL+V GRIW+
Sbjct: 285 ERKELKLRVTGRIWK 299

BLAST of Tan0000821 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.3e-123
Identity = 196/265 (73.96%), Postives = 229/265 (86.42%), Query Frame = 0

Query: 60  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 119
           +A  G       VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 120 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 179
           IRCLALEKVAESLELPC + +LGC ++FPYYSKLKHE  C +RPY+CPYAG ECSVTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 180 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 239
           P+LV HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 240 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 299
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 300 RKLALFCSGNEGQELKLKVAGRIWR 325
           R +ALF SG + +ELKL+V GRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

BLAST of Tan0000821 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 436.4 bits (1121), Expect = 2.8e-121
Identity = 190/253 (75.10%), Postives = 222/253 (87.75%), Query Frame = 0

Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC N M+PPI+QC NGHTLCS+CK+RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 40  VYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAES 99

Query: 132 LELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHE 191
           LELPC Y NLGC+ +FPYYSKLKHE  C +RPY+CPYAG EC+  GDI +LV HL+DDH+
Sbjct: 100 LELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHK 159

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG APVYMAFLRFM
Sbjct: 160 VDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFM 219

Query: 252 GDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD+DDA+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +ALF SG + 
Sbjct: 220 GDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDK 279

Query: 312 QELKLKVAGRIWR 325
           +ELKL+V GRIW+
Sbjct: 280 KELKLRVTGRIWK 292

BLAST of Tan0000821 vs. NCBI nr
Match: XP_038900383.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 656.0 bits (1691), Expect = 1.7e-184
Identity = 306/325 (94.15%), Postives = 316/325 (97.23%), Query Frame = 0

Query: 1   MSTVEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKAS 60
           M+TVEFREKGMYQQQKSREE A SAASFCKEVTELRL SIDSQAATSRPE +G++FRKAS
Sbjct: 13  MTTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAS 72

Query: 61  AGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
             GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNI
Sbjct: 73  T-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNI 132

Query: 121 RCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 180
           RCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP
Sbjct: 133 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIP 192

Query: 181 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 193 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 252

Query: 241 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 253 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 312

Query: 301 KLALFCSGNEGQELKLKVAGRIWRV 326
           KLALFCSGNEG ELKLKV GRIWRV
Sbjct: 313 KLALFCSGNEGMELKLKVTGRIWRV 336

BLAST of Tan0000821 vs. NCBI nr
Match: XP_038900384.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_038900386.1 E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 656.0 bits (1691), Expect = 1.7e-184
Identity = 306/325 (94.15%), Postives = 316/325 (97.23%), Query Frame = 0

Query: 1   MSTVEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKAS 60
           M+TVEFREKGMYQQQKSREE A SAASFCKEVTELRL SIDSQAATSRPE +G++FRKAS
Sbjct: 1   MTTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAS 60

Query: 61  AGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
             GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNI
Sbjct: 61  T-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 180
           RCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIP 180

Query: 181 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGQELKLKVAGRIWRV 326
           KLALFCSGNEG ELKLKV GRIWRV
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRV 324

BLAST of Tan0000821 vs. NCBI nr
Match: XP_038900382.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 656.0 bits (1691), Expect = 1.7e-184
Identity = 306/325 (94.15%), Postives = 316/325 (97.23%), Query Frame = 0

Query: 1   MSTVEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKAS 60
           M+TVEFREKGMYQQQKSREE A SAASFCKEVTELRL SIDSQAATSRPE +G++FRKAS
Sbjct: 13  MTTVEFREKGMYQQQKSREEHAFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKAS 72

Query: 61  AGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
             GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKAR+QNCCPTCRHELGNI
Sbjct: 73  T-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNI 132

Query: 121 RCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 180
           RCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP
Sbjct: 133 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIP 192

Query: 181 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 193 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 252

Query: 241 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQR
Sbjct: 253 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 312

Query: 301 KLALFCSGNEGQELKLKVAGRIWRV 326
           KLALFCSGNEG ELKLKV GRIWRV
Sbjct: 313 KLALFCSGNEGMELKLKVTGRIWRV 336

BLAST of Tan0000821 vs. NCBI nr
Match: XP_008449780.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 646.0 bits (1665), Expect = 1.8e-181
Identity = 305/327 (93.27%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MST-VEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKA 60
           MST +EFREKGMYQQQKSREE ASSAASFCKEVTEL L SIDSQ ATSRPE +GV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120
           S  GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN
Sbjct: 61  ST-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120

Query: 121 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 180
           IRCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDI
Sbjct: 121 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 180

Query: 181 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240
           P LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG
Sbjct: 181 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240

Query: 241 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 300
           TAPVYMAFLRFMGDD++AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQ
Sbjct: 241 TAPVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 300

Query: 301 RKLALFCSGNEGQELKLKVAGRIWRVR 327
           RKLALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 RKLALFCSGNEGMELKLKVTGRIWRVR 326

BLAST of Tan0000821 vs. NCBI nr
Match: XP_031740164.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetical protein Csa_018085 [Cucumis sativus])

HSP 1 Score: 645.2 bits (1663), Expect = 3.0e-181
Identity = 305/327 (93.27%), Postives = 314/327 (96.02%), Query Frame = 0

Query: 1   MST-VEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKA 60
           MST +EFREKGMYQQ KSREE ASSAASFCKEVTELRL SIDSQ ATSRPE +GV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120
           S  GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN
Sbjct: 61  ST-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120

Query: 121 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 180
           IRCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDI
Sbjct: 121 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 180

Query: 181 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240
           P LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG
Sbjct: 181 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240

Query: 241 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 300
           TAPVYMAFLRFMGDD +AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQ
Sbjct: 241 TAPVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 300

Query: 301 RKLALFCSGNEGQELKLKVAGRIWRVR 327
           RKLALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 RKLALFCSGNEGMELKLKVTGRIWRVR 326

BLAST of Tan0000821 vs. ExPASy TrEMBL
Match: A0A1S3BMT4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 8.6e-182
Identity = 305/327 (93.27%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MST-VEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKA 60
           MST +EFREKGMYQQQKSREE ASSAASFCKEVTEL L SIDSQ ATSRPE +GV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120
           S  GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN
Sbjct: 61  ST-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120

Query: 121 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 180
           IRCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDI
Sbjct: 121 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 180

Query: 181 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240
           P LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG
Sbjct: 181 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240

Query: 241 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 300
           TAPVYMAFLRFMGDD++AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQ
Sbjct: 241 TAPVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 300

Query: 301 RKLALFCSGNEGQELKLKVAGRIWRVR 327
           RKLALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 RKLALFCSGNEGMELKLKVTGRIWRVR 326

BLAST of Tan0000821 vs. ExPASy TrEMBL
Match: A0A0A0KWV9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.5e-181
Identity = 305/327 (93.27%), Postives = 314/327 (96.02%), Query Frame = 0

Query: 1   MST-VEFREKGMYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKA 60
           MST +EFREKGMYQQ KSREE ASSAASFCKEVTELRL SIDSQ ATSRPE +GV+FRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120
           S  GGFAR+RNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN
Sbjct: 61  ST-GGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120

Query: 121 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 180
           IRCLALEKVAESLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDI
Sbjct: 121 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 180

Query: 181 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240
           P LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG
Sbjct: 181 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240

Query: 241 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 300
           TAPVYMAFLRFMGDD +AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQ
Sbjct: 241 TAPVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 300

Query: 301 RKLALFCSGNEGQELKLKVAGRIWRVR 327
           RKLALFCSGNEG ELKLKV GRIWRVR
Sbjct: 301 RKLALFCSGNEGMELKLKVTGRIWRVR 326

BLAST of Tan0000821 vs. ExPASy TrEMBL
Match: A0A5D3DDZ5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00330 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 9.8e-178
Identity = 296/316 (93.67%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 11  MYQQQKSREERASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKASAGGGFARNRN 70
           MYQQQKSREE ASSAASFCKEVTEL L SIDSQ ATSRPE +GV+FRKAS  GGFAR+RN
Sbjct: 1   MYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKAST-GGFARSRN 60

Query: 71  DVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAE 130
           DVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAE
Sbjct: 61  DVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAE 120

Query: 131 SLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDH 190
           SLELPC+Y+NLGC+DMFPYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP LVMHLKDDH
Sbjct: 121 SLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDH 180

Query: 191 EVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 250
           EVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF
Sbjct: 181 EVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 240

Query: 251 MGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNE 310
           MGDD++AKQFSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDSHDGLIIQRKLALFCSGNE
Sbjct: 241 MGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNE 300

Query: 311 GQELKLKVAGRIWRVR 327
           G ELKLKV GRIWRVR
Sbjct: 301 GMELKLKVTGRIWRVR 315

BLAST of Tan0000821 vs. ExPASy TrEMBL
Match: A0A6J1DI06 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021266 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.2e-171
Identity = 288/327 (88.07%), Postives = 302/327 (92.35%), Query Frame = 0

Query: 1   MSTVEFREKGMYQQQKSREER-ASSAASFCKEVTELRLASIDSQAATSRPECRGVSFRKA 60
           MS VEFREK MYQQ+K REE  +SS ASFCKEVTELRL SIDSQAATSRPE + VS RK+
Sbjct: 1   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 60

Query: 61  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 120
           S   GF RN N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGN
Sbjct: 61  ST--GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGN 120

Query: 121 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 180
           IRCL LEKVAESLELPC+Y+NLGC+D  PYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI
Sbjct: 121 IRCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 180

Query: 181 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 240
           PYLVMHLKDDHEVDMH+GCSFSHRYVKSNP+EVENATWMLTVFNCFGRQFCLHFEAFRLG
Sbjct: 181 PYLVMHLKDDHEVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLG 240

Query: 241 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 300
           TAPVYMAFLRFMGDDDDAK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII 
Sbjct: 241 TAPVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIH 300

Query: 301 RKLALFCSGNEGQELKLKVAGRIWRVR 327
           R LALFCSGN GQELKLKV GRIWRVR
Sbjct: 301 RNLALFCSGNHGQELKLKVVGRIWRVR 325

BLAST of Tan0000821 vs. ExPASy TrEMBL
Match: A0A5C7IUW2 (RING-type E3 ubiquitin transferase OS=Acer yangbiense OX=1000413 GN=EZV62_001706 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.4e-141
Identity = 234/297 (78.79%), Postives = 260/297 (87.54%), Query Frame = 0

Query: 29  CKEVTELRLASIDSQAATSRPECRGVSFRKASAG-GGFARNRNDVHELLDCTVCMNLMHP 88
           CKE+ E R+A  D +  TS  E RG   RKA+ G GG   + NDVHELL+C VCMNLM+P
Sbjct: 8   CKEIIESRIAFADYETTTSNVELRGSPCRKATTGFGGNTTSTNDVHELLECPVCMNLMYP 67

Query: 89  PIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCLYENLGCVDMF 148
           PIYQC NGHT+CS+CK RV+N CPTCRHELGNIRCLALEKVAESLELPC Y+ LGC D+F
Sbjct: 68  PIYQCPNGHTVCSNCKVRVRNSCPTCRHELGNIRCLALEKVAESLELPCRYQILGCHDIF 127

Query: 149 PYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGCSFSHRYVKS 208
           PYY KLKHEKNCKYRPYNCPYAG ECSVTGDIP+LVMHLK+DH+VDMHDG +F+HRYVK+
Sbjct: 128 PYYGKLKHEKNCKYRPYNCPYAGAECSVTGDIPFLVMHLKNDHKVDMHDGSTFNHRYVKA 187

Query: 209 NPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEVG 268
           N QEVENATWMLTVFNCFGRQFCLHFEAF LG+APVYMAFLRFMGD+D+A+QFSY+LEVG
Sbjct: 188 NAQEVENATWMLTVFNCFGRQFCLHFEAFHLGSAPVYMAFLRFMGDEDEARQFSYTLEVG 247

Query: 269 GNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGQELKLKVAGRIWR 325
           GNGRKL WQG+PRSIRDSHRKVRDS DGLIIQR LALF SG + QELKLKVAGRIW+
Sbjct: 248 GNGRKLTWQGIPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDKQELKLKVAGRIWK 304

BLAST of Tan0000821 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.1e-131
Identity = 218/301 (72.43%), Postives = 251/301 (83.39%), Query Frame = 0

Query: 27  SFCKEVTELRLASIDSQAATSRPECRGVSFRK-ASAGGG--FARNRNDVHELLDCTVCMN 86
           S  KEV E     +D +  T++ E       K  SAG G     + N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 87  LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCLYENLGC 146
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 147 VDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGCSFSHR 206
            D+FPYYSKLKHE++C++RPY CPYAG ECSVTGDIP LV+HLKDDH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 207 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYS 266
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 267 LEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGQELKLKVAGRIW 325
           LEVG +GRKL WQG+PRSIRDSHRKVRDS DGLII R LAL+ SG + QELKL+V GRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

BLAST of Tan0000821 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 463.8 bits (1192), Expect = 1.2e-130
Identity = 211/298 (70.81%), Postives = 246/298 (82.55%), Query Frame = 0

Query: 27  SFCKEVTELRLASIDSQAATSRPECRGVSFRKASAGGGFARNRNDVHELLDCTVCMNLMH 86
           S  KE  E     +D +   ++ E      +  S   G   + N V+ELL+C VC NLM+
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMY 65

Query: 87  PPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCLYENLGCVDM 146
           PPI+QC NGHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC Y+NLGC D+
Sbjct: 66  PPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDI 125

Query: 147 FPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHDGCSFSHRYVK 206
           FPYYSKLKHE++C++R Y+CPYAG ECSVTGDIP LV HLKDDH+VDMHDGC+F+HRYVK
Sbjct: 126 FPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVK 185

Query: 207 SNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKQFSYSLEV 266
           SNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AK+FSYSLEV
Sbjct: 186 SNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEV 245

Query: 267 GGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGQELKLKVAGRIWR 325
           G + RKL WQG+PRSIRDSHRKVRDS DGLII R LAL+ SG++ +ELKL+V GRIW+
Sbjct: 246 GAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

BLAST of Tan0000821 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 444.1 bits (1141), Expect = 9.6e-125
Identity = 196/265 (73.96%), Postives = 229/265 (86.42%), Query Frame = 0

Query: 60  SAGGGFARNRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 119
           +A  G       VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 120 IRCLALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDI 179
           IRCLALEKVAESLELPC + +LGC ++FPYYSKLKHE  C +RPY+CPYAG ECSVTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 180 PYLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 239
           P+LV HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 240 TAPVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQ 299
            APVYMAFLRFMGD+ +A+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 300 RKLALFCSGNEGQELKLKVAGRIWR 325
           R +ALF SG + +ELKL+V GRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

BLAST of Tan0000821 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 427.2 bits (1097), Expect = 1.2e-119
Identity = 187/253 (73.91%), Postives = 221/253 (87.35%), Query Frame = 0

Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC   M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 58  VYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAES 117

Query: 132 LELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHE 191
           LELPC + NLGC ++FPYYSKLKHE  C +RPY+CPYAG EC + GDIP+LV HL+DDH+
Sbjct: 118 LELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHK 177

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRFM
Sbjct: 178 VDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFM 237

Query: 252 GDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD++DA+ +SYSLEVGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQR +ALF SG + 
Sbjct: 238 GDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDR 297

Query: 312 QELKLKVAGRIWR 325
           +ELKL+V G+IW+
Sbjct: 298 KELKLRVTGKIWK 310

BLAST of Tan0000821 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.5e-96
Identity = 152/202 (75.25%), Postives = 178/202 (88.12%), Query Frame = 0

Query: 123 LALEKVAESLELPCLYENLGCVDMFPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYL 182
           +ALEKVAESLELPC Y NLGC+ +FPYYSKLKHE  C +RPY+CPYAG EC+  GDI +L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 183 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 242
           V HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 243 VYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGMPRSIRDSHRKVRDSHDGLIIQRKL 302
           VYMAFLRFMGD+DDA+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 303 ALFCSGNEGQELKLKVAGRIWR 325
           ALF SG + +ELKL+V GRIW+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWK 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2P41.5e-13072.43E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
P937481.6e-12970.81Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
Q10L911.6e-12476.86E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q84JL31.3e-12373.96E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q8S3N12.8e-12175.10E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Match NameE-valueIdentityDescription
XP_038900383.11.7e-18494.15E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida][more]
XP_038900384.11.7e-18494.15E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_03890... [more]
XP_038900382.11.7e-18494.15E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida][more]
XP_008449780.11.8e-18193.27PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo][more]
XP_031740164.13.0e-18193.27E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetic... [more]
Match NameE-valueIdentityDescription
A0A1S3BMT48.6e-18293.27RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 ... [more]
A0A0A0KWV91.5e-18193.27RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE... [more]
A0A5D3DDZ59.8e-17893.67RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1DI065.2e-17188.07RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102126... [more]
A0A5C7IUW27.4e-14178.79RING-type E3 ubiquitin transferase OS=Acer yangbiense OX=1000413 GN=EZV62_001706... [more]
Match NameE-valueIdentityDescription
AT3G58040.11.1e-13172.43seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.2e-13070.81Protein with RING/U-box and TRAF-like domains [more]
AT3G61790.19.6e-12573.96Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.11.2e-11973.91Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.12.5e-9675.25TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 64..130
e-value: 2.3E-5
score: 26.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 131..186
e-value: 4.7E-16
score: 60.8
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 187..319
e-value: 3.4E-35
score: 122.7
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 120..318
e-value: 2.5E-79
score: 265.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 26..324
NoneNo IPR availablePANTHERPTHR10315:SF123E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKEcoord: 26..324
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 76..114
e-value: 9.06666E-13
score: 59.991
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 131..322
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 69..120
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 78..114
score: 9.214112
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 131..191
score: 13.741816

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000821.1Tan0000821.1mRNA
Tan0000821.2Tan0000821.2mRNA
Tan0000821.3Tan0000821.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding