Tan0000631 (gene) Snake gourd v1

Overview
NameTan0000631
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
LocationLG01: 13183831 .. 13187375 (+)
RNA-Seq ExpressionTan0000631
SyntenyTan0000631
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACCCTAGTCCCTCACCGTTAAAAAAAAAAAAACCCTATACCCCTCATCTTTCATTTTCTACCCCCTTCTTCCCGCCGGCTCTTCCTTTCTTTCAGCAGCTCACTGATTCCGCCGCCGTCGTTCTCCTGCATGTTCCTGCGCCTCCTCCGTCATAGCGGTTTGCACTGATTTTCTACTCTCTTGTTGTTATTGGTATTCTCCCAAATTCCATCCATGTCTTTCTTCTCACTTGAAAAACTTAAATTAGGACTTAGTCTAGTGAAAGAATAGAGTTGTTTTTTTTTTTTTGGTCTCGGCTGAAAATTTCATTGTTTCTAGTCTCCCACATCTTCCAAAGCATCCCCCACTGTGAATTTTTGAGCATCTCCCTCCTTTATTTGTCTCGAATCAGTCGAAGTAATCTATCACACACCAATTTTCCCTATTAAACAAAAATTCCCAGATATTAGAATTAAAGTTGTTATAACCAAATTTCTTTTCGAAACTTACACCGCATGGCAGGTAGTTTCGATTTTACTCTTTTTGTTTTTAGCGTCAACTTGTTCAATACAATCTGGTCCTGGACAGACTTGGAAGTTCTTGTATTGGTTGTTTTCTGGGGTTTGAACGTTGGAATTTGCTTTATCAATTTTCTACTGTAATTTTGCGTTTGCGCTGAGTGCTTTTTTTAATTCCCAATTCAAATACTTTCTCCAACCCCTCTACTATTTCATTCTGATTCTGCTCAAATTCTAGTTGGCGCTCTTTTTCCCTGCTTTCTATTTATTATTTGTTGCTTGCCAACCGACTCAGATTTGCATCTTGAGGAGTAAATTCTCCATAAACATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTGCCCTCAGTCTTGTTACTGCTCTCGCATTCTTTTGATTATAATTTTCTGGTCTGTCAACTCTCTCTGAGTCTCTTTTGTAGACTTGTAATCTGCAATATCTTGACTTTGCTGTGAATGATTTTACATGACGTTTACTGACTGCATAAAATTGTTCTTTTAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGTAATATTTTCCCCCTTAATGTTTAATTGAATTTCGTACCATGCTGGAGCTCGTGTCATTTGTTTCATGTTTCGACAGATACACTTTTCTGCATTTATATGCATCTTAAAGATTCTAAAAGAAACAACTAATAATTCCATAAACTTTATCTGATACTGAAAATTTGGAGAAGGATTTTACCGAACAGTCCCAAAAAAATTAGATAAGAGGGTCACTGAACTGCAAGGCAAGTATCCATTGGTTCTGTTCTGTTAAGGTTGTGCTAGCCATGTCTCTTAAAAAGAAATGAGAGAAAAGGAAAGGTCATTTAAGTAGGTGAGATAAGGCTTGTGTCCAAGGCTTGTGACGGATGGTATAGACTTGTTTAATACTGAAGACACTCGCCTTATGCTCCTTGAAAATATGGAGGAGTATTTTCTCAGTCTCAACAGAACTCTTCAAGATGAGCGTCTTTGTAGGATCAATTTGTTGGAGGAGTTCCTTGGTAGTCTCCCGGGAAAGGGGTCTGGTTCTCTTGCCATCGGTTTTTTGCTGCCTCGTTGCATTCATGGCTCAAAAGAAATTGGTGAGTCTTTAGGGATATAGAGAAGTCATGAAGAGGAGGTTTGGACTCTTTGGAGTGAGTTGCATTTCAATGCCTCTCTCTAGGTGTGCTTGTTTAACTTTGGGAACAAACTGCTGTAATTTTCCTTTTGTTATGTTTTGTAAGCTACTTTGTCTGGCCCATTCTTTTTCGTCATCCGCTCCTTTTGAGAAAACATATATATTCTCTTAGGAAGAAAATATATATGTTTTCTTTTTATAATTTGACTAGGTTTCTATAAACTTTCCATAAAGTATTAAAAAAAAAAGAACAGTGATTATAAGCAAACAACTTTATGTTCTAGACGCAAAACTAAAAACAGAATTCTGCAAATGTTCCTTAGTATGTGAAGTGCCGAGGATGTGCTAACAAGAATCGCTTTGACAAACCTGTTTTACTTCAGCTGTCAAGAATGTGAACATAATTTAAATCTCTAGTAATAGTAATATCTTTCAATTGAACTTAATGTTTTTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTGTAAGTGAACTTGCATTACCATGATTTTCCTCAACGATTTGAATCTGCTATTTTTTTTTTGGTTTGGGGGTTCAAATATCCTAAATGTATAACTTAGTCCCTTATTTGTGCCTTTGAACAAAATGCTAAAATAAGAAAGAATGATCATTTATAAATTTGGAAAAGATATTTTTTAAGTGGTTGATGTTAGACTATGTTGATATAATTAAATTTACCCCAAACTCTCAGCTTAAGCTTTTAGTTTGATTGGTGATTTAAGATGGTATCAGAGTGGGTGGTCCAGGAGATCCTGTGTTCAAGCCTTGCAAAGTCAGTTTCCTCCCCATTTAATCTTGATTTACCCCAAACTCTCAGCTTAAGCTTTTAGTTTGATTGGTGATTTAAGAGTTTACTCACCACTATCTGCCCATTTTGGTGGATACATTTTGTATAACATCAAACCATTTTTGGGTTAGATAAGTGTAAGGTGAACTCCCTATACTATATGGTTTGATATTCAAAATGAAAACAAGCAAGAATAAAACAAGTGCCCTACGAATTTGAGAGGGTGTGAGAGTACTAACAGGAGATATGTTGGAAATATTTGTAGTGTAGTAATAAAGACATAGTGGTAATTAGTTAGGAGTGACTTGGTTATAAATAGAGGGAGTTTAGGGGCCTTAGAAGGGCGATGGTTTTGTGTATTGTCTAGTAAGGAAACAAACACAATTTTTAAGTTATCAAATGGGGCATTATAATTCTAACCTTTCTTAAAGTGTAAGTATTTTTTTTTAATATGTCATCTCATAAAAAATATAGTATATTTTCAACTCAATTAATAAAATACATTTTAATTCTGATGTTGTTTGGTTGGAATGGTTGTATTTGCAGAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGAACTAATTATGGCCTTTTTTTATTACAGAAATTGTAATAACTCTGTTTTTTTTTTTTTTTTACTTATAGTTAATTTATGAATTATGAAAACTTTCACTAAATTGATCTTCATAGTTTCTGTATGGTTTAACATACATGGGTAAATTTTATTTGAAATTCTTTTTTCTTTCCCTTCTAAAAAA

mRNA sequence

AAACCCTAGTCCCTCACCGTTAAAAAAAAAAAAACCCTATACCCCTCATCTTTCATTTTCTACCCCCTTCTTCCCGCCGGCTCTTCCTTTCTTTCAGCAGCTCACTGATTCCGCCGCCGTCGTTCTCCTGCATGTTCCTGCGCCTCCTCCGTCATAGCGATTTGCATCTTGAGGAGTAAATTCTCCATAAACATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGAACTAATTATGGCCTTTTTTTATTACAGAAATTGTAATAACTCTGTTTTTTTTTTTTTTTTACTTATAGTTAATTTATGAATTATGAAAACTTTCACTAAATTGATCTTCATAGTTTCTGTATGGTTTAACATACATGGGTAAATTTTATTTGAAATTCTTTTTTCTTTCCCTTCTAAAAAA

Coding sequence (CDS)

ATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGA

Protein sequence

MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLKGDPLHCAKFRMDQSMFLLLRKIT
Homology
[Close]
BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 82/142 (57.75%), Postives = 106/142 (74.65%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GD+  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 5.8e-42
Identity = 80/142 (56.34%), Postives = 106/142 (74.65%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EA S  L M+M LDVNTE+YP+  GD+  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.6e-30
Identity = 65/144 (45.14%), Postives = 97/144 (67.36%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  +LF+D F+VK ++PDGKK+D+VSR   +SE   M + +D+ T IYP+   +R  MVL
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           + +LN DG+   S S    K SLADK++Y+M+GK++K   E S S  KV V+ASFGGL +
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENSSS--KVSVFASFGGLLV 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRK 145
           +L+GDP +     +D  ++LL++K
Sbjct: 121 LLQGDPRYLPGIELDARIYLLIKK 141

[Close]
BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-29
Identity = 59/145 (40.69%), Postives = 99/145 (68.28%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  ++FDD+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + D++ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRMDQSMFLLLRK 145
           M LKG+ ++   F +D +++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.1e-28
Identity = 58/145 (40.00%), Postives = 97/145 (66.90%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  ++FDD+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + D+  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRMDQSMFLLLRK 145
           M LKG+ ++   F +D +++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of Tan0000631 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 285.4 bits (729), Expect = 2.7e-73
Identity = 138/146 (94.52%), Postives = 144/146 (98.63%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M E+LFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Tan0000631 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.5 bits (724), Expect = 1.0e-72
Identity = 137/146 (93.84%), Postives = 143/146 (97.95%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Tan0000631 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 279.6 bits (714), Expect = 1.5e-71
Identity = 136/145 (93.79%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH KLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDPLHC KFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]
BLAST of Tan0000631 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.9 bits (712), Expect = 2.6e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Tan0000631 vs. NCBI nr
Match: XP_038901286.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 2.2e-70
Identity = 132/144 (91.67%), Postives = 141/144 (97.92%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIF VKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFDDIFMVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSY+GQEGKKSLADKFEYIMHGK+YKLSD+GSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYTGQEGKKSLADKFEYIMHGKIYKLSDDGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRK 145
           +LKGDP+HCAKFR+DQ+MFLLLRK
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRK 144

[Close]
BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1J6Q9 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111481850 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.3e-73
Identity = 138/146 (94.52%), Postives = 144/146 (98.63%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M E+LFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1FVR2 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447307 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 7.3e-72
Identity = 136/145 (93.79%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH KLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDPLHC KFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]
BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1C3H4 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=3673 GN=LOC111008140 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.4e-69
Identity = 129/145 (88.97%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           MVELLFDDIFKVKCVNPDG+KYDKVSRI+A SEKL+M+MLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGKLYKLS+EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Tan0000631 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 174.1 bits (440), Expect = 8.3e-44
Identity = 82/142 (57.75%), Postives = 106/142 (74.65%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GD+  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Tan0000631 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 171.8 bits (434), Expect = 4.1e-43
Identity = 80/142 (56.34%), Postives = 106/142 (74.65%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EA S  L M+M LDVNTE+YP+  GD+  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:
BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O810971.2e-4257.75DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q9M1A85.8e-4256.34DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
Q54YW86.6e-3045.14DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc... [more]
Q198261.9e-2940.69Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
A8XT812.1e-2840.00Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
BLAST of Tan0000631 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022983196.12.7e-7394.52DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP... [more]
KAG6600626.11.0e-7293.84DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros... [more]
XP_022942165.11.5e-7193.79DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata][more]
XP_022929200.12.6e-7191.72DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >... [more]
XP_038901286.12.2e-7091.67DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida][more]
BLAST of Tan0000631 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1J6Q91.3e-7394.52DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
A0A6J1FVR27.3e-7293.79DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1ETP81.2e-7191.72DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1J4541.2e-7191.72DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
A0A6J1C3H44.4e-6988.97DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=... [more]
BLAST of Tan0000631 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G54250.18.3e-4457.75RNA polymerase Rpb8 [more]
AT3G59600.14.1e-4356.34RNA polymerase Rpb8 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..144
e-value: 6.1E-56
score: 201.8
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..144
e-value: 6.7E-49
score: 165.6
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..146
e-value: 1.2E-46
score: 156.4
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..145
NoneNo IPR availableGENE3D2.40.50.140coord: 6..146
e-value: 5.3E-53
score: 180.9
NoneNo IPR availablePANTHERPTHR10917:SF1DNA-DIRECTED RNA POLYMERASE I, IIcoord: 1..145
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 4..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000631.1Tan0000631.1mRNA
Tan0000631.2Tan0000631.2mRNA
Tan0000631.3Tan0000631.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity