Tan0000608 (gene) Snake gourd v1

Overview
NameTan0000608
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionHeavy metal-associated isoprenylated plant protein
LocationLG09: 73569414 .. 73571563 (+)
RNA-Seq ExpressionTan0000608
SyntenyTan0000608
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCGAAGGAAACCCTGGTAAGGATCCGAGTTGTGTTTGTGTCGTAAATATCGATGCAGAATGTGAACGTGGCCATTATAAAAAGCCACGTCACATGAGGAAGATGACGATGATGATAATAGAATTGAGAGAGATCTGGAAGTGAATGCCATTCCTCTTACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTGAGCTTCATCCATTCCAATGGGGGAGGTCTCTAATTTCCCCAACTCTGTTTCTTTCTCTGTTTTCACATTTCGTTTATCAGTTCATTTTCTTACTCTCTGGATTTATGTTCATTCATTAGGAGAACGACAAGAAACCAGAGGAACACCCGAAAGCGGCTTCCGATCAAGAGATAAAAGAGGCCGAGAACAACCCACCGGAGAAGAAAGAAGCAGAGGCCATCCAAGAACAGCCTCTGATTCCGAGTCCGAGTCTGAGTCTTCCTCAACCCCCTCAGCATCAGGATATTGTCCTCACTGTTTTTATGCATTGCGAAGGCTGCGCCCGCAAGGTCCGCCGCTGCCTCAAAGGCTTCGAAGGTTAGATTGTTATGTTCTTTAATTTACTGAAATTAGTAAAGATTAATAATTGATTGATCTATGATCTTTCTTCTTTTTTCATAGGGATTGAAAGTGTGGAGACCGATTGTAAAACTCACAGAGTGGTGGTGAAAGGGGAGAAGGCTGATCCGCTGAAGATTTTGGGCAGACTTCAGAGGAAAAGCCATAGACGGGTCGAATTGATCTCCCCAATTCCAGAGCCCCCTGCTCCGAATCCCGAACCCATTGAGAAGCCCAAAACAGAGGAGAAGAAACCAGAGGTTCTATTCTACTTCTACTTCTACTTCTACGGTTTATTTCTCAATATATATCTCTCTTCTTACATTTTCGATTTCATTTTGTTTGCTACTTTTGAAATTCGCAGCGTCAGATTATAACAGTCGTCTTGAAAGTGCACATGCATTGCGAGGCTTGTGCTCAAGAAATCAAAAGAAGGATACACAGGATGAAAGGTTTCATTTTACTTACTTCTTTCTCATATAACTTTACTTTACTACACTACATCTGATTTGGATATATATATTTATATATTACACGAGATGGATCTTCTAAGTTCTATTATATATTAATAAAAAAGACTTGATTGTTTGACTAATCTTTCACTTTCGTCACCACATTCTTCAATATCGGAAATACGCCAAATTTAATTACTACTCCTATTTTTATTTTTTAATCGTAAAATTTGTTGTTGGAAATTTAATGCGTCAGGTGTTGAATCGGTGAACCCGGATCTTAAAAGCTCGCAGGTCACGGTGAAAGGATCGATCGATCCAGCGGCGCTGGTTCAATATGTTCACCGGAGAACCGGAAAGCACGCGGCGATTATGAAGCAAGAGCCGGAAATTACGCCGGAGAATAATTCTGAAGATGCTGCAAAAGAAGACAAAGAAGACAAAAAAGCCGATGCGGGCGACGGCGGGGGAGGCGAAAGTGAGAAGAAGGTGGACGAAGAAAGTAAAGTCGAAGAGAAAGCCGTCGCCGCCGCCACTGCCACCGAAGGAGAAGCACAGGGAGCCGCCGATCCGGCGGCGGGAGAAGCTCTGAAGGTGGTGGAAGTGAAGAAGAATGAATATCACTGTTACCCGCAAAGGTATATAATGGAAATGTATCCGTATCCACCGCCGATGATTGGCGACGTGGCCTACCCGCCGCCGCATATGGTGGTAGACGCCTATCCGCCGCAGGTGATCGGCCACGCATATCCACCTCAGATGTTCAGCGATGAGAATCCAAATGCCTGTTCCGTCATGTGAAGTAAAACGGAGAAAATATAATATTTTGACAAAACCAAAAAAAAAAAAAAATCCTTTAATTTCTGCTTCATCTATACGGTGTTCTTCATTCCAATGGGCCAGAAGTATTAAATATACAATCAACGGATGTGGATGAGCCGTGGAATTTTGTGAATGTTATAAAGTTTCCTACCATATGTTATAAAATAATGGCGTATATATCATTGGTACACGATGGTTACAATCATATATTATAAGGGGCCGACCGAATTACAACACAGAATCTCAAAAGGCAGAAATTATTGCGTTCGTATAATGAAGTGTTAGATTGTAATGGGTG

mRNA sequence

GTCGAAGGAAACCCTGGTAAGGATCCGAGTTGTGTTTGTGTCGTAAATATCGATGCAGAATGTGAACGTGGCCATTATAAAAAGCCACGTCACATGAGGAAGATGACGATGATGATAATAGAATTGAGAGAGATCTGGAAGTGAATGCCATTCCTCTTACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCTGAGCTTCATCCATTCCAATGGGGGAGGAGAACGACAAGAAACCAGAGGAACACCCGAAAGCGGCTTCCGATCAAGAGATAAAAGAGGCCGAGAACAACCCACCGGAGAAGAAAGAAGCAGAGGCCATCCAAGAACAGCCTCTGATTCCGAGTCCGAGTCTGAGTCTTCCTCAACCCCCTCAGCATCAGGATATTGTCCTCACTGTTTTTATGCATTGCGAAGGCTGCGCCCGCAAGGTCCGCCGCTGCCTCAAAGGCTTCGAAGGGATTGAAAGTGTGGAGACCGATTGTAAAACTCACAGAGTGGTGGTGAAAGGGGAGAAGGCTGATCCGCTGAAGATTTTGGGCAGACTTCAGAGGAAAAGCCATAGACGGGTCGAATTGATCTCCCCAATTCCAGAGCCCCCTGCTCCGAATCCCGAACCCATTGAGAAGCCCAAAACAGAGGAGAAGAAACCAGAGCGTCAGATTATAACAGTCGTCTTGAAAGTGCACATGCATTGCGAGGCTTGTGCTCAAGAAATCAAAAGAAGGATACACAGGATGAAAGGTGTTGAATCGGTGAACCCGGATCTTAAAAGCTCGCAGGTCACGGTGAAAGGATCGATCGATCCAGCGGCGCTGGTTCAATATGTTCACCGGAGAACCGGAAAGCACGCGGCGATTATGAAGCAAGAGCCGGAAATTACGCCGGAGAATAATTCTGAAGATGCTGCAAAAGAAGACAAAGAAGACAAAAAAGCCGATGCGGGCGACGGCGGGGGAGGCGAAAGTGAGAAGAAGGTGGACGAAGAAAGTAAAGTCGAAGAGAAAGCCGTCGCCGCCGCCACTGCCACCGAAGGAGAAGCACAGGGAGCCGCCGATCCGGCGGCGGGAGAAGCTCTGAAGGTGGTGGAAGTGAAGAAGAATGAATATCACTGTTACCCGCAAAGGTATATAATGGAAATGTATCCGTATCCACCGCCGATGATTGGCGACGTGGCCTACCCGCCGCCGCATATGGTGGTAGACGCCTATCCGCCGCAGGTGATCGGCCACGCATATCCACCTCAGATGTTCAGCGATGAGAATCCAAATGCCTGTTCCGTCATGTGAAGTAAAACGGAGAAAATATAATATTTTGACAAAACCAAAAAAAAAAAAAAATCCTTTAATTTCTGCTTCATCTATACGGTGTTCTTCATTCCAATGGGCCAGAAGTATTAAATATACAATCAACGGATGTGGATGAGCCGTGGAATTTTGTGAATGTTATAAAGTTTCCTACCATATGTTATAAAATAATGGCGTATATATCATTGGTACACGATGGTTACAATCATATATTATAAGGGGCCGACCGAATTACAACACAGAATCTCAAAAGGCAGAAATTATTGCGTTCGTATAATGAAGTGTTAGATTGTAATGGGTG

Coding sequence (CDS)

ATGGGGGAGGAGAACGACAAGAAACCAGAGGAACACCCGAAAGCGGCTTCCGATCAAGAGATAAAAGAGGCCGAGAACAACCCACCGGAGAAGAAAGAAGCAGAGGCCATCCAAGAACAGCCTCTGATTCCGAGTCCGAGTCTGAGTCTTCCTCAACCCCCTCAGCATCAGGATATTGTCCTCACTGTTTTTATGCATTGCGAAGGCTGCGCCCGCAAGGTCCGCCGCTGCCTCAAAGGCTTCGAAGGGATTGAAAGTGTGGAGACCGATTGTAAAACTCACAGAGTGGTGGTGAAAGGGGAGAAGGCTGATCCGCTGAAGATTTTGGGCAGACTTCAGAGGAAAAGCCATAGACGGGTCGAATTGATCTCCCCAATTCCAGAGCCCCCTGCTCCGAATCCCGAACCCATTGAGAAGCCCAAAACAGAGGAGAAGAAACCAGAGCGTCAGATTATAACAGTCGTCTTGAAAGTGCACATGCATTGCGAGGCTTGTGCTCAAGAAATCAAAAGAAGGATACACAGGATGAAAGGTGTTGAATCGGTGAACCCGGATCTTAAAAGCTCGCAGGTCACGGTGAAAGGATCGATCGATCCAGCGGCGCTGGTTCAATATGTTCACCGGAGAACCGGAAAGCACGCGGCGATTATGAAGCAAGAGCCGGAAATTACGCCGGAGAATAATTCTGAAGATGCTGCAAAAGAAGACAAAGAAGACAAAAAAGCCGATGCGGGCGACGGCGGGGGAGGCGAAAGTGAGAAGAAGGTGGACGAAGAAAGTAAAGTCGAAGAGAAAGCCGTCGCCGCCGCCACTGCCACCGAAGGAGAAGCACAGGGAGCCGCCGATCCGGCGGCGGGAGAAGCTCTGAAGGTGGTGGAAGTGAAGAAGAATGAATATCACTGTTACCCGCAAAGGTATATAATGGAAATGTATCCGTATCCACCGCCGATGATTGGCGACGTGGCCTACCCGCCGCCGCATATGGTGGTAGACGCCTATCCGCCGCAGGTGATCGGCCACGCATATCCACCTCAGATGTTCAGCGATGAGAATCCAAATGCCTGTTCCGTCATGTGA

Protein sequence

MGEENDKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEIKRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAKEDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPNACSVM
Homology
BLAST of Tan0000608 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 8.2e-74
Identity = 188/357 (52.66%), Postives = 229/357 (64.15%), Query Frame = 0

Query: 6   DKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQHQDIVLTVFM 65
           +KK  E  K  ++++ ++++  P E +  +  +E     +P    P PP  Q++VL V+M
Sbjct: 23  EKKEGEDKKVDAEKKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPP-QEVVLKVYM 82

Query: 66  HCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRRVELISP 125
           HCEGCARKVRRCLKGFEG+E V TDCKT +VVVKGEKADPLK+L R+QRK+HR+V+L+SP
Sbjct: 83  HCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSP 142

Query: 126 IPEPPAPNPEPIE--KPKTEEKKPERQ-IITVVLKVHMHCEACAQEIKRRIHRMKGVESV 185
           IP PP P  +  E  KP  EEKK E   ++TVVLKVHMHCEACA EIK+RI RMKGVES 
Sbjct: 143 IPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESA 202

Query: 186 NPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAKEDKEDKKA 245
             DLKSSQVTVKG  +P  LV+YV++RTGKHAAIMK +P   P    E AA  + E K+ 
Sbjct: 203 ESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPP-PPPPPEEAAAAAEGEKKEE 262

Query: 246 DAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEAL-KVVEVKKNEYHC 305
           + G+G     E K D+    EEK        EGE   AAD   GE   KVVEV+K E   
Sbjct: 263 EKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGE---AADNGGGEEEGKVVEVRKIENPY 322

Query: 306 YPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPNACSVM 359
           Y        Y Y PP    VA PP  M     PP    HAYPPQ+FSDENPNAC+VM
Sbjct: 323 Y-------YYYYQPPR---VAIPPMEM-----PP----HAYPPQLFSDENPNACTVM 355

BLAST of Tan0000608 vs. ExPASy Swiss-Prot
Match: Q9M8T7 (Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=3702 GN=HIPP08 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-36
Identity = 81/185 (43.78%), Postives = 122/185 (65.95%), Query Frame = 0

Query: 55  QHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQR 114
           Q ++IVL V+MHCEGCA +V  CL+G++G+E ++T+   ++VVV G+  DPLKIL R+Q+
Sbjct: 34  QCKEIVLKVYMHCEGCASQVSHCLRGYDGVEHIKTEIGDNKVVVSGKFDDPLKILRRVQK 93

Query: 115 KSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEIKRRIH 174
           K  R  E+ISP   P     EP +K   +E  PE  I T +L+++MHCE C  EIKR I 
Sbjct: 94  KFSRNAEMISPKHNPKQDQKEPQQK---KESAPE--IKTAILRMNMHCEGCVHEIKRGIE 153

Query: 175 RMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAK 234
           ++KG++SV PD   S V V+G +DP  LV+ + ++ GKHA ++ Q  E   +NN ++  K
Sbjct: 154 KIKGIQSVEPDRSKSTVVVRGVMDPPKLVEKIKKKLGKHAELLSQITEKGKDNNKKNNNK 213

Query: 235 EDKED 240
           +++ D
Sbjct: 214 KEESD 213

BLAST of Tan0000608 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-28
Identity = 115/361 (31.86%), Postives = 183/361 (50.69%), Query Frame = 0

Query: 1   MGEENDKKPEEHPKAASDQ----EIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQH 60
           MGEE   KPE    A++ Q    E +E + +  E+K+  A +E+P         P PP  
Sbjct: 1   MGEE--VKPEAKEAASAPQAVPAEEEEKKKDVAEEKKVAAEEEKPKEEEEPQPPPPPP-- 60

Query: 61  QDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKS 120
              +L V +HC GCA+K+ R +    G+E V  D   ++V +KG   DP  +  ++++K+
Sbjct: 61  -PFILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNENQVTIKG-VLDPQAVCNKIKKKT 120

Query: 121 HRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEIKRRIHRM 180
            R  +++SP+   PA   EP+  P     +    + TV L V+MHC+ACA ++K++I +M
Sbjct: 121 KRMAKVLSPL---PAAEGEPL--PPIITSQVSGGLTTVELSVNMHCQACADQLKKKILKM 180

Query: 181 KGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAKED 240
           +GV++   +  + +V V G++D   LV YV+RRT K A I+   P+  PE  +  AA+E+
Sbjct: 181 RGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARIV---PQPDPEPEAPAAAQEE 240

Query: 241 KEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEALKVVEVKK 300
           K+++  +  +      E+K +E+ K  E+         GE  G  + AA E     E + 
Sbjct: 241 KKEESGEGNEKPPETGEEKEEEKKKEGEE--------NGEEGGGEEAAATEE----ERRD 300

Query: 301 NEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPNACS 358
           NE     Q   M+   Y         Y  P  V++  P        PPQ+FSDENPNAC 
Sbjct: 301 NEMTAMAQEEGMKRMMY---------YYQPSYVIERIP--------PPQLFSDENPNACC 318

BLAST of Tan0000608 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.5e-27
Identity = 97/313 (30.99%), Postives = 149/313 (47.60%), Query Frame = 0

Query: 59  IVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHR 118
           +VL V MHCEGCA ++ +C++ F+G+E+V+++  T ++ V G   DP+K+  +L+ K+ +
Sbjct: 28  VVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTG-ALDPVKLREKLEEKTKK 87

Query: 119 RVELISPIP----EPPAPNPEPIEKPKTEEKK---------PERQIITVVLKVHMHCEAC 178
           +V+L+SP P    E    N    +K K+EEKK          E  + T VLK++ HC+ C
Sbjct: 88  KVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETPVTTAVLKLNFHCQGC 147

Query: 179 AQEIKRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITP 238
             +I++ + + KGV  +  D + + +TVKG++D   LV+ +  +  +   I+  + E   
Sbjct: 148 IGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEKLKRAVEIVPPKKEKDK 207

Query: 239 ENNSEDAAKEDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAA 298
           EN +E+      E KK   GDGGG E                   T  +G  +G      
Sbjct: 208 ENGNENG-----EKKKGGGGDGGGKEK------------------TGNKGGGEG------ 267

Query: 299 GEALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQ 358
                   V   EY      Y    YP  P                 YP Q    A+ PQ
Sbjct: 268 --------VNMMEYMAAQPAYGYGYYPGGP---------------YGYPIQ----AHAPQ 283

BLAST of Tan0000608 vs. ExPASy Swiss-Prot
Match: Q9SHQ8 (Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=3702 GN=HIPP04 PE=3 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 96/305 (31.48%), Postives = 154/305 (50.49%), Query Frame = 0

Query: 60  VLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRR 119
           V  V +HC  CA  +++ L  F+G+++V+ D + + + VKG K + +KI  ++++ S ++
Sbjct: 18  VYKVHLHCRKCACDIKKPLLRFQGVQNVDFDLEKNEIKVKG-KIEVVKIHKQIEKWSKKK 77

Query: 120 VELISPIPE------PPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEIKRRI 179
           VELISP P               +EK  TE KK    I T VLKVH+HC  C ++++ ++
Sbjct: 78  VELISPKPSEVKKTTTTTTTTSVVEKKTTEIKKD--VIRTTVLKVHIHCAQCDKDLQHKL 137

Query: 180 HRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAA 239
            + K +  V  D K+  +TV+G+I+ A L+ Y+ ++  KHA I+  + E   +   ED  
Sbjct: 138 LKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYIKKKVHKHAEIISSKTEEEKKKEEEDKK 197

Query: 240 KEDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEALKVVE 299
           K+++EDKK         E EKK +EE K EE+        EGE +        E +KV  
Sbjct: 198 KKEEEDKKKK-------EDEKKKEEEKKKEEE----NKKKEGEKK-------KEEVKVEV 257

Query: 300 VKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPN 359
             K          I ++  Y      ++         D   P  + + Y PQ+FSDENPN
Sbjct: 258 TTKT---------ITQVVEYK-----EIVKVEGQKDKDGNIPYFVHYVYAPQLFSDENPN 287

BLAST of Tan0000608 vs. NCBI nr
Match: XP_038878612.1 (heavy metal-associated isoprenylated plant protein 7 [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 6.2e-135
Identity = 279/368 (75.82%), Postives = 309/368 (83.97%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQH 60
           MGEE+    D KPEE P+AA DQE   AE++  E K  +A++E+P        LP+PP  
Sbjct: 1   MGEEDKKPEDTKPEEPPRAAPDQEQTGAESDNKESK--DAVEEKP--------LPKPP-- 60

Query: 61  QDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKS 120
           QDIVL+VFMHCEGCARKVRRCL+GFEG+ESVETDC+TH+VVVKGEKADP+K+LGRLQRKS
Sbjct: 61  QDIVLSVFMHCEGCARKVRRCLRGFEGVESVETDCRTHKVVVKGEKADPVKVLGRLQRKS 120

Query: 121 HRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQII-TVVLKVHMHCEACAQEIKRRIHR 180
           HRRVELISPIPEP AP PEP+EKPKTE+ KP+ QI+ TVVLKVHMHCEACAQEIKRRIHR
Sbjct: 121 HRRVELISPIPEPTAPIPEPVEKPKTEDTKPQPQILTTVVLKVHMHCEACAQEIKRRIHR 180

Query: 181 MKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENN-SEDAAK 240
           MKGVE V+PDLKSSQVTVKG+IDPAALV YVHRRTGKHAAI+KQEPEITPENN SE AAK
Sbjct: 181 MKGVELVDPDLKSSQVTVKGAIDPAALVAYVHRRTGKHAAIVKQEPEITPENNESEVAAK 240

Query: 241 EDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATA---TEGEAQGAADPAAGEALKV 300
           E +E+KKADA D  G ESEKKV+EESKVEEK  AAA      EG A   ADP A EA K+
Sbjct: 241 EVEEEKKADASD--GVESEKKVEEESKVEEKPAAAAAGGGEAEGTAPAEADPVAEEAPKM 300

Query: 301 VEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYP-PQVIGHAYPPQMFSDE 359
           VEVKKNEYH YPQRYIMEMYPY PPM+GDVAYP PHMVVDAYP P ++GHAYPPQMFSDE
Sbjct: 301 VEVKKNEYHYYPQRYIMEMYPYAPPMMGDVAYPAPHMVVDAYPAPMMMGHAYPPQMFSDE 354

BLAST of Tan0000608 vs. NCBI nr
Match: XP_022921978.1 (heavy metal-associated isoprenylated plant protein 7 [Cucurbita moschata])

HSP 1 Score: 472.2 bits (1214), Expect = 3.9e-129
Identity = 270/372 (72.58%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENN---PPEKKEAE----AIQEQPLIPSPSLS 60
           MGEE+    D KPEE  KAA DQ+ K+ ++    PPE K+AE     I+EQP        
Sbjct: 1   MGEEDNKPADSKPEEPLKAAPDQDNKQPQSENPPPPENKQAEEPKDGIKEQP-------- 60

Query: 61  LPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKIL 120
           LPQPP  QDIVL VFMHCEGCARKVRRCLKGFEG+ESVETDC+TH+VVVKGEKADPLK+L
Sbjct: 61  LPQPP--QDIVLAVFMHCEGCARKVRRCLKGFEGVESVETDCRTHKVVVKGEKADPLKVL 120

Query: 121 GRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEI 180
           GRLQRKSHRRVELISP+PE PA NP P EKPKTE+ KPE QI+TVVLKVHMHCEACAQEI
Sbjct: 121 GRLQRKSHRRVELISPVPELPAANPPPEEKPKTEDSKPEPQIVTVVLKVHMHCEACAQEI 180

Query: 181 KRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNS 240
           KRRIHRMKGVESV+PDLKSSQVTVKG+IDPAALV+YVHRRTGKHAAI+KQEPEI PENNS
Sbjct: 181 KRRIHRMKGVESVDPDLKSSQVTVKGAIDPAALVEYVHRRTGKHAAIVKQEPEIAPENNS 240

Query: 241 EDAAKEDKEDKKADAGD--GGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGE 300
           +D AKE KE+KKA AGD  G G ESEKKV+EESKVEE     A A +GE     DP    
Sbjct: 241 DDGAKEVKEEKKASAGDGVGEGAESEKKVEEESKVEE-IPGGADAGDGETAAEEDP---- 300

Query: 301 ALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMF 359
             KVVEVKKNE+H YPQRYIMEMYPYP P++  + YP  HM VDAYP +VIG+ YPPQMF
Sbjct: 301 --KVVEVKKNEHHYYPQRYIMEMYPYPAPIMDGIGYPAAHMAVDAYPARVIGYEYPPQMF 355

BLAST of Tan0000608 vs. NCBI nr
Match: XP_022988250.1 (heavy metal-associated isoprenylated plant protein 7 [Cucurbita maxima])

HSP 1 Score: 462.2 bits (1188), Expect = 4.0e-126
Identity = 265/372 (71.24%), Postives = 295/372 (79.30%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENN---PPEKKEAE----AIQEQPLIPSPSLS 60
           MGEE+    D KPEE P AA D+E +E ++    PPEK  AE     I+EQP        
Sbjct: 1   MGEEDNKPVDSKPEEPPNAAPDRENEEPQSENPPPPEKTPAEEPKDGIKEQP-------- 60

Query: 61  LPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKIL 120
           LP+PP  QDIVLTVFMHCEGCARKVRRCLKGFEG+ESVETDC+TH+VVVKGEKADPLK+L
Sbjct: 61  LPRPP--QDIVLTVFMHCEGCARKVRRCLKGFEGVESVETDCRTHKVVVKGEKADPLKVL 120

Query: 121 GRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEI 180
           GRLQRKSHRRVELISP+PE PA NP P EKPKTE+ KPE QI+TVVLKVHMHCEACAQEI
Sbjct: 121 GRLQRKSHRRVELISPVPELPAANPPPEEKPKTEDNKPEPQIVTVVLKVHMHCEACAQEI 180

Query: 181 KRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNS 240
           +RRIHRMKGVESV+PDLKSSQVTVKG+IDPAALV+YVHRRTGKHAAI+KQEPEI PENNS
Sbjct: 181 RRRIHRMKGVESVDPDLKSSQVTVKGAIDPAALVEYVHRRTGKHAAIVKQEPEIAPENNS 240

Query: 241 EDAAKEDKEDKKADAGDGG--GGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGE 300
           +D AKE KE+KKA+AGDG   G E EKKV+EESKVEE          G   G    AA E
Sbjct: 241 DDGAKEVKEEKKANAGDGDGEGAECEKKVEEESKVEE-------IPGGGDAGDGTTAAEE 300

Query: 301 ALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMF 359
             KVVEVKKNE+H YPQRYIMEMYPYP P++  + YP  H+ VDA P +VIG+ YPPQMF
Sbjct: 301 DPKVVEVKKNEHHYYPQRYIMEMYPYPAPIMDGIGYPAAHIAVDACPARVIGYEYPPQMF 355

BLAST of Tan0000608 vs. NCBI nr
Match: KAG7023054.1 (Heavy metal-associated isoprenylated plant protein 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.8 bits (1187), Expect = 5.2e-126
Identity = 266/376 (70.74%), Postives = 295/376 (78.46%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENN---PPEKKEAE----AIQEQPLIPSPSLS 60
           MGEE+    D KPEE  KAA DQE K+ ++    PPE K+AE     I+EQP        
Sbjct: 1   MGEEDNKPADSKPEEPQKAAPDQENKQPQSENPPPPENKQAEEPKDGIKEQP-------- 60

Query: 61  LPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKIL 120
           LPQPP  QDIVLTVFMHCEGCARKVRRCLKGFEG+ESVETDC+TH+VVVKGEKADPLK+L
Sbjct: 61  LPQPP--QDIVLTVFMHCEGCARKVRRCLKGFEGVESVETDCRTHKVVVKGEKADPLKVL 120

Query: 121 GRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEI 180
           GRLQRKSHRRVELISP+PE PA NP   EKPKTE+ KPE QI+TVVLKVHMHCEACAQEI
Sbjct: 121 GRLQRKSHRRVELISPVPELPAANPPLEEKPKTEDNKPEPQIVTVVLKVHMHCEACAQEI 180

Query: 181 KRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNS 240
           KRRIHRMKGVESV+PDLKSSQVTVKG+IDP ALV+YVHRRTGKHAAI+KQEPEI PENNS
Sbjct: 181 KRRIHRMKGVESVDPDLKSSQVTVKGAIDPVALVEYVHRRTGKHAAIVKQEPEIAPENNS 240

Query: 241 EDAAKEDKEDKKADAGDGG------GGESEKKVDEESKVEEKAVAAATATEGEAQGAADP 300
           +D AKE KE+ KA+AGDG       G +SE KV+EESKVEE     A   +GE     DP
Sbjct: 241 DDGAKEVKEEIKANAGDGDGDGDGEGAKSENKVEEESKVEE-IPGGADGGDGETAAEEDP 300

Query: 301 AAGEALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYP 359
                 KVVEVKKNE+H YPQRYIMEMYPYP P++  + YP  HM VDAYP +VIG+ YP
Sbjct: 301 ------KVVEVKKNEHHYYPQRYIMEMYPYPAPIMDGIGYPAAHMAVDAYPARVIGYEYP 359

BLAST of Tan0000608 vs. NCBI nr
Match: KAG6589371.1 (Heavy metal-associated isoprenylated plant protein 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 460.7 bits (1184), Expect = 1.2e-125
Identity = 266/376 (70.74%), Postives = 295/376 (78.46%), Query Frame = 0

Query: 1   MGEENDK----KPEEHPKAASDQEIKEAENN---PPEKKEAE----AIQEQPLIPSPSLS 60
           MGEE++K    KPEE  KAA DQE K+ ++    PPE K+AE     I+EQP        
Sbjct: 1   MGEEDNKPAGSKPEEPQKAAPDQENKQPQSENPPPPENKQAEEPKDGIKEQP-------- 60

Query: 61  LPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKIL 120
           LPQPP  QDIVLTVFMHCEGCARKVRRCLKGFEG+ESVETDC TH+VVVKGEKADPLK+L
Sbjct: 61  LPQPP--QDIVLTVFMHCEGCARKVRRCLKGFEGVESVETDCTTHKVVVKGEKADPLKVL 120

Query: 121 GRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEI 180
           GRLQRKSHRRVELISP+PE PA NP   EKPKTE+ KPE QI+TVVLKVHMHCEACAQEI
Sbjct: 121 GRLQRKSHRRVELISPVPELPAANPPLEEKPKTEDNKPEPQIVTVVLKVHMHCEACAQEI 180

Query: 181 KRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNS 240
           KRRIHRMKGVESV+PDLKSSQVTVKG+IDP ALV+YVHRRTGKHAAI+KQEPEI PENNS
Sbjct: 181 KRRIHRMKGVESVDPDLKSSQVTVKGAIDPVALVEYVHRRTGKHAAIVKQEPEIAPENNS 240

Query: 241 EDAAKEDKEDKKADAGDGG------GGESEKKVDEESKVEEKAVAAATATEGEAQGAADP 300
           +D AKE KE+ KA+AGDG       G +SE KV+EESKVEE     A   +GE     DP
Sbjct: 241 DDGAKEVKEEIKANAGDGDGDGDGEGAKSENKVEEESKVEE-IPGGADGGDGETAAEEDP 300

Query: 301 AAGEALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYP 359
                 KVVEVKKNE+H YPQRYIMEMYPYP P++  + YP  HM VDAYP +VIG+ YP
Sbjct: 301 ------KVVEVKKNEHHYYPQRYIMEMYPYPAPIMDGIGYPAAHMAVDAYPARVIGYEYP 359

BLAST of Tan0000608 vs. ExPASy TrEMBL
Match: A0A6J1E5A8 (heavy metal-associated isoprenylated plant protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111430071 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.9e-129
Identity = 270/372 (72.58%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENN---PPEKKEAE----AIQEQPLIPSPSLS 60
           MGEE+    D KPEE  KAA DQ+ K+ ++    PPE K+AE     I+EQP        
Sbjct: 1   MGEEDNKPADSKPEEPLKAAPDQDNKQPQSENPPPPENKQAEEPKDGIKEQP-------- 60

Query: 61  LPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKIL 120
           LPQPP  QDIVL VFMHCEGCARKVRRCLKGFEG+ESVETDC+TH+VVVKGEKADPLK+L
Sbjct: 61  LPQPP--QDIVLAVFMHCEGCARKVRRCLKGFEGVESVETDCRTHKVVVKGEKADPLKVL 120

Query: 121 GRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEI 180
           GRLQRKSHRRVELISP+PE PA NP P EKPKTE+ KPE QI+TVVLKVHMHCEACAQEI
Sbjct: 121 GRLQRKSHRRVELISPVPELPAANPPPEEKPKTEDSKPEPQIVTVVLKVHMHCEACAQEI 180

Query: 181 KRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNS 240
           KRRIHRMKGVESV+PDLKSSQVTVKG+IDPAALV+YVHRRTGKHAAI+KQEPEI PENNS
Sbjct: 181 KRRIHRMKGVESVDPDLKSSQVTVKGAIDPAALVEYVHRRTGKHAAIVKQEPEIAPENNS 240

Query: 241 EDAAKEDKEDKKADAGD--GGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGE 300
           +D AKE KE+KKA AGD  G G ESEKKV+EESKVEE     A A +GE     DP    
Sbjct: 241 DDGAKEVKEEKKASAGDGVGEGAESEKKVEEESKVEE-IPGGADAGDGETAAEEDP---- 300

Query: 301 ALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMF 359
             KVVEVKKNE+H YPQRYIMEMYPYP P++  + YP  HM VDAYP +VIG+ YPPQMF
Sbjct: 301 --KVVEVKKNEHHYYPQRYIMEMYPYPAPIMDGIGYPAAHMAVDAYPARVIGYEYPPQMF 355

BLAST of Tan0000608 vs. ExPASy TrEMBL
Match: A0A6J1JJ25 (heavy metal-associated isoprenylated plant protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111485558 PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.9e-126
Identity = 265/372 (71.24%), Postives = 295/372 (79.30%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENN---PPEKKEAE----AIQEQPLIPSPSLS 60
           MGEE+    D KPEE P AA D+E +E ++    PPEK  AE     I+EQP        
Sbjct: 1   MGEEDNKPVDSKPEEPPNAAPDRENEEPQSENPPPPEKTPAEEPKDGIKEQP-------- 60

Query: 61  LPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKIL 120
           LP+PP  QDIVLTVFMHCEGCARKVRRCLKGFEG+ESVETDC+TH+VVVKGEKADPLK+L
Sbjct: 61  LPRPP--QDIVLTVFMHCEGCARKVRRCLKGFEGVESVETDCRTHKVVVKGEKADPLKVL 120

Query: 121 GRLQRKSHRRVELISPIPEPPAPNPEPIEKPKTEEKKPERQIITVVLKVHMHCEACAQEI 180
           GRLQRKSHRRVELISP+PE PA NP P EKPKTE+ KPE QI+TVVLKVHMHCEACAQEI
Sbjct: 121 GRLQRKSHRRVELISPVPELPAANPPPEEKPKTEDNKPEPQIVTVVLKVHMHCEACAQEI 180

Query: 181 KRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNS 240
           +RRIHRMKGVESV+PDLKSSQVTVKG+IDPAALV+YVHRRTGKHAAI+KQEPEI PENNS
Sbjct: 181 RRRIHRMKGVESVDPDLKSSQVTVKGAIDPAALVEYVHRRTGKHAAIVKQEPEIAPENNS 240

Query: 241 EDAAKEDKEDKKADAGDGG--GGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGE 300
           +D AKE KE+KKA+AGDG   G E EKKV+EESKVEE          G   G    AA E
Sbjct: 241 DDGAKEVKEEKKANAGDGDGEGAECEKKVEEESKVEE-------IPGGGDAGDGTTAAEE 300

Query: 301 ALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMF 359
             KVVEVKKNE+H YPQRYIMEMYPYP P++  + YP  H+ VDA P +VIG+ YPPQMF
Sbjct: 301 DPKVVEVKKNEHHYYPQRYIMEMYPYPAPIMDGIGYPAAHIAVDACPARVIGYEYPPQMF 355

BLAST of Tan0000608 vs. ExPASy TrEMBL
Match: A0A0A0LU05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001430 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.6e-125
Identity = 269/374 (71.93%), Postives = 304/374 (81.28%), Query Frame = 0

Query: 1   MGEEN----DKKPEEHPKAASDQEIKEAENNPPEKKEA-EAIQEQPLIPSPSLSLPQPPQ 60
           MGEE+    D K EE PKAA DQE  E E+   EKKE+ + I+E+P        LP+PP 
Sbjct: 1   MGEEDRKPEDTKLEEPPKAAPDQEKTEGES---EKKESKDVIEEKP--------LPKPP- 60

Query: 61  HQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRK 120
            QDIVL+VFMHCEGCARKVRRCL+GFEG+ESVETDC+TH+VVVKGEKADP+K+L RLQRK
Sbjct: 61  -QDIVLSVFMHCEGCARKVRRCLRGFEGVESVETDCRTHKVVVKGEKADPVKVLNRLQRK 120

Query: 121 SHRRVELISPIPEPP--APNPEPIEKPKTEEKKPERQII-TVVLKVHMHCEACAQEIKRR 180
           SHRRVELISPIPEP   AP PEP+EK KTE+ KP+ QII TVVLKVHMHCEACAQEIKRR
Sbjct: 121 SHRRVELISPIPEPEPIAPVPEPVEKLKTEDPKPQPQIIVTVVLKVHMHCEACAQEIKRR 180

Query: 181 IHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSED- 240
           IHRMKGVESV+PDLKSSQV+VKG+ DPAALV YVHRRTGKHAAI+KQEPE+TPENN  + 
Sbjct: 181 IHRMKGVESVDPDLKSSQVSVKGAFDPAALVAYVHRRTGKHAAIVKQEPEVTPENNESEV 240

Query: 241 -AAKEDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEAL- 300
            A KE +E+KKADAGD  G ESEKKV+EES VEEK  AAA   +GEA+ AA   AG+A  
Sbjct: 241 VAVKEAEEEKKADAGD--GVESEKKVEEESVVEEKP-AAAPPGDGEAEEAAPGDAGQAAA 300

Query: 301 ----KVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQV-IGHAYPP 359
               K+VEVKKNEYH YPQRYIMEMYPY PP+IGD +YPPP M V+ YPP V +GHAYPP
Sbjct: 301 EEGPKMVEVKKNEYHYYPQRYIMEMYPYAPPVIGDTSYPPPQMAVETYPPPVMMGHAYPP 358

BLAST of Tan0000608 vs. ExPASy TrEMBL
Match: A0A5D3BID4 (Heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00420 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 8.1e-125
Identity = 269/377 (71.35%), Postives = 306/377 (81.17%), Query Frame = 0

Query: 1   MGEE----NDKKPEEHPKAASDQEIKEAENNPPEKKEA-EAIQEQPLIPSPSLSLPQPPQ 60
           MGEE     D KPEE PKAA DQE  E E+   EKKE+ + I+E+P        LP+PP 
Sbjct: 1   MGEEVKKVEDTKPEEPPKAAPDQEKTEGES---EKKESKDVIEEKP--------LPKPP- 60

Query: 61  HQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRK 120
            +DIVL+VFMHCEGCARKVRRCL+GFEG+ESVETDC+TH+VVVKGEKADP+K+L RLQRK
Sbjct: 61  -RDIVLSVFMHCEGCARKVRRCLRGFEGVESVETDCRTHKVVVKGEKADPVKVLNRLQRK 120

Query: 121 SHRRVELISPIPEPP--APNPEPIEKPKTEEKKPE-RQIITVVLKVHMHCEACAQEIKRR 180
           SHRRVELISPIPEP   AP PEP+EKPKTE+ KP+ R I+TVVLKVHMHCEACAQEIKRR
Sbjct: 121 SHRRVELISPIPEPEPIAPIPEPVEKPKTEDPKPQPRIIVTVVLKVHMHCEACAQEIKRR 180

Query: 181 IHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSED- 240
           IHRMKGVESV+PDLKSSQV+VKG+ DPAALV YVHRRTGKHAAI+KQEPE+T ENN+E  
Sbjct: 181 IHRMKGVESVDPDLKSSQVSVKGAFDPAALVAYVHRRTGKHAAIVKQEPEVTLENNNESE 240

Query: 241 --AAKEDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAA-ATATEGEAQG-----AADP 300
             A KE +E+KKADAGD  G ESEKKV+EES VEEK  AA A   +GEA+G     AA  
Sbjct: 241 AVAVKEVEEEKKADAGD--GVESEKKVEEESTVEEKPAAADAAPGDGEAEGASPGEAAQQ 300

Query: 301 AAGEALKVVEVKKNEYH-CYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYP-PQVIGHA 359
           AA E  K+VEVKKNEYH  YPQRYIMEMYPY PP++GD+AYP P MV++ YP P ++GHA
Sbjct: 301 AAEEGPKMVEVKKNEYHYYYPQRYIMEMYPYAPPVMGDIAYPAPQMVMETYPAPAMMGHA 360

BLAST of Tan0000608 vs. ExPASy TrEMBL
Match: A0A1S3CLJ3 (heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo OX=3656 GN=LOC103502307 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 8.1e-125
Identity = 269/377 (71.35%), Postives = 306/377 (81.17%), Query Frame = 0

Query: 1   MGEE----NDKKPEEHPKAASDQEIKEAENNPPEKKEA-EAIQEQPLIPSPSLSLPQPPQ 60
           MGEE     D KPEE PKAA DQE  E E+   EKKE+ + I+E+P        LP+PP 
Sbjct: 1   MGEEVKKVEDTKPEEPPKAAPDQEKTEGES---EKKESKDVIEEKP--------LPKPP- 60

Query: 61  HQDIVLTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRK 120
            +DIVL+VFMHCEGCARKVRRCL+GFEG+ESVETDC+TH+VVVKGEKADP+K+L RLQRK
Sbjct: 61  -RDIVLSVFMHCEGCARKVRRCLRGFEGVESVETDCRTHKVVVKGEKADPVKVLNRLQRK 120

Query: 121 SHRRVELISPIPEPP--APNPEPIEKPKTEEKKPE-RQIITVVLKVHMHCEACAQEIKRR 180
           SHRRVELISPIPEP   AP PEP+EKPKTE+ KP+ R I+TVVLKVHMHCEACAQEIKRR
Sbjct: 121 SHRRVELISPIPEPEPIAPIPEPVEKPKTEDPKPQPRIIVTVVLKVHMHCEACAQEIKRR 180

Query: 181 IHRMKGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSED- 240
           IHRMKGVESV+PDLKSSQV+VKG+ DPAALV YVHRRTGKHAAI+KQEPE+T ENN+E  
Sbjct: 181 IHRMKGVESVDPDLKSSQVSVKGAFDPAALVAYVHRRTGKHAAIVKQEPEVTLENNNESE 240

Query: 241 --AAKEDKEDKKADAGDGGGGESEKKVDEESKVEEKAVAA-ATATEGEAQG-----AADP 300
             A KE +E+KKADAGD  G ESEKKV+EES VEEK  AA A   +GEA+G     AA  
Sbjct: 241 AVAVKEVEEEKKADAGD--GVESEKKVEEESTVEEKPAAADAAPGDGEAEGASPGEAAQQ 300

Query: 301 AAGEALKVVEVKKNEYH-CYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYP-PQVIGHA 359
           AA E  K+VEVKKNEYH  YPQRYIMEMYPY PP++GD+AYP P MV++ YP P ++GHA
Sbjct: 301 AAEEGPKMVEVKKNEYHYYYPQRYIMEMYPYAPPVMGDIAYPAPQMVMETYPAPAMMGHA 360

BLAST of Tan0000608 vs. TAIR 10
Match: AT5G63530.1 (farnesylated protein 3 )

HSP 1 Score: 278.9 bits (712), Expect = 5.8e-75
Identity = 188/357 (52.66%), Postives = 229/357 (64.15%), Query Frame = 0

Query: 6   DKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQHQDIVLTVFM 65
           +KK  E  K  ++++ ++++  P E +  +  +E     +P    P PP  Q++VL V+M
Sbjct: 23  EKKEGEDKKVDAEKKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPP-QEVVLKVYM 82

Query: 66  HCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRRVELISP 125
           HCEGCARKVRRCLKGFEG+E V TDCKT +VVVKGEKADPLK+L R+QRK+HR+V+L+SP
Sbjct: 83  HCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSP 142

Query: 126 IPEPPAPNPEPIE--KPKTEEKKPERQ-IITVVLKVHMHCEACAQEIKRRIHRMKGVESV 185
           IP PP P  +  E  KP  EEKK E   ++TVVLKVHMHCEACA EIK+RI RMKGVES 
Sbjct: 143 IPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESA 202

Query: 186 NPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAKEDKEDKKA 245
             DLKSSQVTVKG  +P  LV+YV++RTGKHAAIMK +P   P    E AA  + E K+ 
Sbjct: 203 ESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPP-PPPPPEEAAAAAEGEKKEE 262

Query: 246 DAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEAL-KVVEVKKNEYHC 305
           + G+G     E K D+    EEK        EGE   AAD   GE   KVVEV+K E   
Sbjct: 263 EKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGE---AADNGGGEEEGKVVEVRKIENPY 322

Query: 306 YPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPNACSVM 359
           Y        Y Y PP    VA PP  M     PP    HAYPPQ+FSDENPNAC+VM
Sbjct: 323 Y-------YYYYQPPR---VAIPPMEM-----PP----HAYPPQLFSDENPNACTVM 355

BLAST of Tan0000608 vs. TAIR 10
Match: AT5G63530.2 (farnesylated protein 3 )

HSP 1 Score: 278.9 bits (712), Expect = 5.8e-75
Identity = 188/357 (52.66%), Postives = 229/357 (64.15%), Query Frame = 0

Query: 6   DKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQHQDIVLTVFM 65
           +KK  E  K  ++++ ++++  P E +  +  +E     +P    P PP  Q++VL V+M
Sbjct: 8   EKKEGEDKKVDAEKKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPP-QEVVLKVYM 67

Query: 66  HCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRRVELISP 125
           HCEGCARKVRRCLKGFEG+E V TDCKT +VVVKGEKADPLK+L R+QRK+HR+V+L+SP
Sbjct: 68  HCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSP 127

Query: 126 IPEPPAPNPEPIE--KPKTEEKKPERQ-IITVVLKVHMHCEACAQEIKRRIHRMKGVESV 185
           IP PP P  +  E  KP  EEKK E   ++TVVLKVHMHCEACA EIK+RI RMKGVES 
Sbjct: 128 IPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESA 187

Query: 186 NPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAKEDKEDKKA 245
             DLKSSQVTVKG  +P  LV+YV++RTGKHAAIMK +P   P    E AA  + E K+ 
Sbjct: 188 ESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPP-PPPPPEEAAAAAEGEKKEE 247

Query: 246 DAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEAL-KVVEVKKNEYHC 305
           + G+G     E K D+    EEK        EGE   AAD   GE   KVVEV+K E   
Sbjct: 248 EKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGE---AADNGGGEEEGKVVEVRKIENPY 307

Query: 306 YPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPNACSVM 359
           Y        Y Y PP    VA PP  M     PP    HAYPPQ+FSDENPNAC+VM
Sbjct: 308 Y-------YYYYQPPR---VAIPPMEM-----PP----HAYPPQLFSDENPNACTVM 340

BLAST of Tan0000608 vs. TAIR 10
Match: AT5G50740.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 172/362 (47.51%), Postives = 219/362 (60.50%), Query Frame = 0

Query: 1   MGEENDKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQHQDIV 60
           MGEE DKK EE  K + + + K  +  P E+K+ E                     Q+IV
Sbjct: 1   MGEE-DKKMEE--KKSEEPQAKSEDKKPEEEKKKEP--------------------QEIV 60

Query: 61  LTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRRV 120
           L +FMHCEGCA+K+ RCLKGFEG+E V TDCKT +VVVKGEKADPLK+L RLQRKSHR+V
Sbjct: 61  LKIFMHCEGCAKKIHRCLKGFEGVEDVTTDCKTSKVVVKGEKADPLKVLQRLQRKSHRQV 120

Query: 121 ELISPIPEPPAPNPEP----IEKPKTEEKKPERQIITVVLKVHMHCEACAQEIKRRIHRM 180
           ELISPIPEP   + EP     EKPK EEKK E  ++TVVL+VHMHCEACA EI++RI RM
Sbjct: 121 ELISPIPEPKPVSDEPEKKEKEKPKPEEKKEE--VVTVVLRVHMHCEACAMEIQKRIMRM 180

Query: 181 KGVESVNPDLKSSQVTVKGSIDPAALVQYVHRRTGKHAAIMKQEPEITPENNSEDAAKED 240
           KGVESV PD K+SQV+VKG   P  LV+++++R GKHAA++KQ+P   P    ++   +D
Sbjct: 181 KGVESVEPDFKASQVSVKGVFTPEKLVEFIYKRIGKHAAVVKQDPPPKPPEKEKET--KD 240

Query: 241 KEDKKADAGDGGGGESEKKVDEESKVEEKAVAAATATEGEAQGAADPAAGEALKVVEVKK 300
           K++KK + G    G+  K+                   G A+G    AA E  KVV++KK
Sbjct: 241 KDEKKKEEGQPKEGKEAKE---------------NGGGGGAKGDG-AAAEEGNKVVDLKK 290

Query: 301 NEYHCYPQRYIMEMYPYPPPMIGDVAYPPPHMVVDAYPPQVIGHAYPPQMFSDENPNACS 359
           NEY   P RY +EM+                             AYPPQ+FSDENPNAC+
Sbjct: 301 NEYQYQPPRYPVEMF-----------------------------AYPPQIFSDENPNACT 290

BLAST of Tan0000608 vs. TAIR 10
Match: AT5G50740.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.3e-71
Identity = 163/333 (48.95%), Postives = 209/333 (62.76%), Query Frame = 0

Query: 30  EKKEAEAIQEQPLIPSPSLSLPQPPQHQDIVLTVFMHCEGCARKVRRCLKGFEGIESVET 89
           E+K++E  Q +     P     + P  Q+IVL +FMHCEGCA+K+ RCLKGFEG+E V T
Sbjct: 2   EEKKSEEPQAKSEDKKPEEEKKKEP--QEIVLKIFMHCEGCAKKIHRCLKGFEGVEDVTT 61

Query: 90  DCKTHRVVVKGEKADPLKILGRLQRKSHRRVELISPIPEPPAPNPEP----IEKPKTEEK 149
           DCKT +VVVKGEKADPLK+L RLQRKSHR+VELISPIPEP   + EP     EKPK EEK
Sbjct: 62  DCKTSKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEEK 121

Query: 150 KPERQIITVVLKVHMHCEACAQEIKRRIHRMKGVESVNPDLKSSQVTVKGSIDPAALVQY 209
           K E  ++TVVL+VHMHCEACA EI++RI RMKGVESV PD K+SQV+VKG   P  LV++
Sbjct: 122 KEE--VVTVVLRVHMHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVEF 181

Query: 210 VHRRTGKHAAIMKQEPEITPENNSEDAAKEDKEDKKADAGDGGGGESEKKVDEESKVEEK 269
           +++R GKHAA++KQ+P   P    ++   +DK++KK + G    G+  K+          
Sbjct: 182 IYKRIGKHAAVVKQDPPPKPPEKEKET--KDKDEKKKEEGQPKEGKEAKE---------- 241

Query: 270 AVAAATATEGEAQGAADPAAGEALKVVEVKKNEYHCYPQRYIMEMYPYPPPMIGDVAYPP 329
                    G A+G    AA E  KVV++KKNEY   P RY +EM+              
Sbjct: 242 -----NGGGGGAKGDG-AAAEEGNKVVDLKKNEYQYQPPRYPVEMF-------------- 283

Query: 330 PHMVVDAYPPQVIGHAYPPQMFSDENPNACSVM 359
                          AYPPQ+FSDENPNAC+++
Sbjct: 302 ---------------AYPPQIFSDENPNACTII 283

BLAST of Tan0000608 vs. TAIR 10
Match: AT5G50740.4 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.8e-45
Identity = 106/183 (57.92%), Postives = 127/183 (69.40%), Query Frame = 0

Query: 1   MGEENDKKPEEHPKAASDQEIKEAENNPPEKKEAEAIQEQPLIPSPSLSLPQPPQHQDIV 60
           MGEE DKK EE  K + + + K  +  P E+K+ E                     Q+IV
Sbjct: 1   MGEE-DKKMEE--KKSEEPQAKSEDKKPEEEKKKEP--------------------QEIV 60

Query: 61  LTVFMHCEGCARKVRRCLKGFEGIESVETDCKTHRVVVKGEKADPLKILGRLQRKSHRRV 120
           L +FMHCEGCA+K+ RCLKGFEG+E V TDCKT +VVVKGEKADPLK+L RLQRKSHR+V
Sbjct: 61  LKIFMHCEGCAKKIHRCLKGFEGVEDVTTDCKTSKVVVKGEKADPLKVLQRLQRKSHRQV 120

Query: 121 ELISPIPEPPAPNPEP----IEKPKTEEKKPERQIITVVLKVHMHCEACAQEIKRRIHRM 180
           ELISPIPEP   + EP     EKPK EEKK E  ++TVVL+VHMHCEACA EI++RI RM
Sbjct: 121 ELISPIPEPKPVSDEPEKKEKEKPKPEEKKEE--VVTVVLRVHMHCEACAMEIQKRIMRM 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5D38.2e-7452.66Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=... [more]
Q9M8T71.8e-3643.78Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=... [more]
Q9FLU51.4e-2831.86Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=... [more]
Q9FJH57.5e-2730.99Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=... [more]
Q9SHQ81.1e-2531.48Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_038878612.16.2e-13575.82heavy metal-associated isoprenylated plant protein 7 [Benincasa hispida][more]
XP_022921978.13.9e-12972.58heavy metal-associated isoprenylated plant protein 7 [Cucurbita moschata][more]
XP_022988250.14.0e-12671.24heavy metal-associated isoprenylated plant protein 7 [Cucurbita maxima][more]
KAG7023054.15.2e-12670.74Heavy metal-associated isoprenylated plant protein 7 [Cucurbita argyrosperma sub... [more]
KAG6589371.11.2e-12570.74Heavy metal-associated isoprenylated plant protein 7, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
A0A6J1E5A81.9e-12972.58heavy metal-associated isoprenylated plant protein 7 OS=Cucurbita moschata OX=36... [more]
A0A6J1JJ251.9e-12671.24heavy metal-associated isoprenylated plant protein 7 OS=Cucurbita maxima OX=3661... [more]
A0A0A0LU051.6e-12571.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001430 PE=3 SV=1[more]
A0A5D3BID48.1e-12571.35Heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo var. makuwa... [more]
A0A1S3CLJ38.1e-12571.35heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo OX=3656 GN=... [more]
Match NameE-valueIdentityDescription
AT5G63530.15.8e-7552.66farnesylated protein 3 [more]
AT5G63530.25.8e-7552.66farnesylated protein 3 [more]
AT5G50740.31.3e-7147.51Heavy metal transport/detoxification superfamily protein [more]
AT5G50740.12.3e-7148.95Heavy metal transport/detoxification superfamily protein [more]
AT5G50740.42.8e-4557.92Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 148..216
e-value: 1.5E-19
score: 72.2
NoneNo IPR availableGENE3D3.30.70.100coord: 54..128
e-value: 2.1E-17
score: 65.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..148
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 215..282
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR46195:SF2HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7coord: 1..358
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 61..115
e-value: 2.8E-12
score: 46.8
coord: 156..212
e-value: 1.4E-14
score: 54.2
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 65..98
score: 8.523955
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 160..206
score: 10.252422
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 155..215
e-value: 5.24047E-10
score: 52.9933
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 65..121
e-value: 5.1627E-11
score: 55.6897
IPR044577Heavy metal-associated isoprenylated plant protein 4/7/8/17/18/19PANTHERPTHR46195HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7coord: 1..358
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 153..215
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 59..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000608.1Tan0000608.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding