Tan0000474 (gene) Snake gourd v1

BLAST of Tan0000474 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 4.4e-85
Identity = 206/428 (48.13%), Postives = 286/428 (66.82%), Query Frame = 0

Query: 28  EDEDADESDRSPSSSSNGEKDEFIVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRE 87
           E+E+ D  +RSPSS S G++ EF+ + L +IRK+VQCPICLGII+KTRTVMECLHRFCRE
Sbjct: 101 EEEEEDSKERSPSSIS-GDQSEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRE 160

Query: 88  CIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFREEERIR 147
           CIDKSMRLGNNECPACR HCASRRSLRDDP +D+LIAAL+ +I+ YEE+ELAF E+E  R
Sbjct: 161 CIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMAR 220

Query: 148 NKQIQASIAQTLRRQSEAVSKKRP--PSIRRLRGNNYMNNHSRGKRSYRNDAEFPAS--- 207
           NKQIQASIAQ  +RQSEA+ K+R        L  +  + + SR +R+ RN  +  AS   
Sbjct: 221 NKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAH 280

Query: 208 --DDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAKFSQPPLAACSDGAGEDN--- 267
             DDN+D       KDSSSDE+   +E R K  R +  ++ +Q P    S GA ++N   
Sbjct: 281 EDDDNDDNNNRGRDKDSSSDER--GTEVRQKKRRKRSTSRSTQHP---SSSGANKNNGNC 340

Query: 268 -DSD-QVHRETLGASSMRVGSSERLVWGKGGMRSHTRHG-STSVGNSKNVKNNRLSALVD 327
            D+D +V+R+T G S   V + E L WG+GG RS+TRHG +TS G+SK+V+N R++ LV+
Sbjct: 341 ADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVE 400

Query: 328 YLRNS-NNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLCQYVAHQTGFQADE 387
           YLR+S + ++VE ++H+ L S D + IP L  PYLCCRPTL V+ L ++VA Q   + +E
Sbjct: 401 YLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEE 460

Query: 388 IDMLLIHELNPKIEPSTSIDVLVSKS-LIPRPTEDNFQVLRERQTLADLKTH--CSTRDQ 439
           +++L+      ++    +I+ L + +      ++D    L + +TL+ LK     S    
Sbjct: 461 VELLVTRR---RVGEDAAIENLPAVTPASAAASKDEMLSLEDNETLSRLKIDFISSHEQH 519

BLAST of Tan0000474 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.7e-74
Identity = 195/459 (42.48%), Postives = 276/459 (60.13%), Query Frame = 0

Query: 3   AQKRAPES--SEDDPLKLDHGCSTARPED----------------EDADESDRSPSSSSN 62
           A++  PE+   E+DP K+D    +   ED                E+ D  +RS SSS+ 
Sbjct: 23  AERFNPEAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKERSQSSSAG 82

Query: 63  G-EKDEFIVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPAC 122
              + E++VV L+DI K+VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECP C
Sbjct: 83  ELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTC 142

Query: 123 RTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQS 182
           R HCASRRSLRDDPN+D+LIAAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS
Sbjct: 143 RKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQS 202

Query: 183 EAVSKKRP--PSIRRLRGNNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDE 242
           +A+ K++        L  +      SR +R+ RN  E   S+ N+D       KDSSSDE
Sbjct: 203 KALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDE 262

Query: 243 QMERSERRLKGFRTQGAAKFSQPPLAACSDGAGEDNDSDQVH-RETLGASSMRVGSSERL 302
             ER  +R K   TQ ++        A ++     N ++Q H R++   S + V +SE +
Sbjct: 263 PCER--QRKKRSATQPSSS------NANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELI 322

Query: 303 VWGKGGMRSHTRHGSTSVGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTI 362
            WG+GG RS+TR G+ + G + + +N RL  LV+YL +   N+VE ++HL L S D   +
Sbjct: 323 AWGRGGTRSNTRQGNNNQG-AISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGL 382

Query: 363 PSLQLPYLCCRPTLSVRHLCQYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSL 422
            +L  PYLC RPTL V+ L +YVA     +A+E+++L+  + +  I   TS         
Sbjct: 383 LNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS--------- 442

Query: 423 IPRPTEDNFQVLRERQTLADLKTHC-STRDQLLLAYQKK 439
                 +  Q L++ +T+A LK  C S+   +++ Y++K
Sbjct: 443 -----TEKMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 457

BLAST of Tan0000474 vs. ExPASy Swiss-Prot
Match: Q6MGB6 (E3 ubiquitin-protein ligase RING1 OS=Rattus norvegicus OX=10116 GN=Ring1 PE=2 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 3.6e-18
Identity = 75/227 (33.04%), Postives = 115/227 (50.66%), Query Frame = 0

Query: 51  IVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 110
           I V    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 111 RSLRDDPNYDSLIAALYPDIEKYE--EDELAFREEERIRNKQIQASIAQTLRRQSEAVSK 170
           RSLR DPN+D+LI+ +YP  E+YE  +D +  R       + + +SI + LR Q    + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQ----AM 154

Query: 171 KRPPSIRR-LRGNNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSE 230
            R   +RR + G++     S G+         P   + +    ED   DS+ D     + 
Sbjct: 155 HRAQRVRRPMPGSDQTTTMSGGEGE-------PGEGEGD---GEDISSDSAPDSAPGPAP 214

Query: 231 RRLKGFRTQGAAKFSQPPLA--ACSDGAGEDNDSDQVHRETLGASSM 273
           +R +G    G++  +    A  AC  GAG ++  D+    TLG  ++
Sbjct: 215 KRPRGGGAGGSSVGTGGGAAGGACG-GAGSEDSGDR--GGTLGGGTL 244

BLAST of Tan0000474 vs. ExPASy Swiss-Prot
Match: Q5TJF3 (E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=3 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.0e-17
Identity = 73/226 (32.30%), Postives = 113/226 (50.00%), Query Frame = 0

Query: 51  IVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 110
           I V    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 111 RSLRDDPNYDSLIAALYPDIEKYE--EDELAFREEERIRNKQIQASIAQTLRRQSEAVSK 170
           RSLR DPN+D+LI+ +YP  E+YE  +D +  R       + + +SI + LR Q    + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQ----AM 154

Query: 171 KRPPSIRR-LRGNNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSE 230
            R   +RR + G++     S G+         P   + +    ED   DS+ D     + 
Sbjct: 155 HRAQRVRRPMPGSDQTTTMSGGEGE-------PGEGEGD---GEDVSSDSAPDSAPGPAP 214

Query: 231 RRLKGFRTQGAAKFSQPPLA-ACSDGAGEDNDSDQVHRETLGASSM 273
           +R +G    G++  +    A     GAG ++  D+    TLG  ++
Sbjct: 215 KRPRGGGAGGSSVGTGGGGAGGVGGGAGSEDSGDR--GGTLGGGTL 244

BLAST of Tan0000474 vs. ExPASy Swiss-Prot
Match: A1YER5 (E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.4e-17
Identity = 72/226 (31.86%), Postives = 111/226 (49.12%), Query Frame = 0

Query: 51  IVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 110
           I V    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 6   IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 65

Query: 111 RSLRDDPNYDSLIAALYPDIEKYE--EDELAFREEERIRNKQIQASIAQTLRRQSEAVSK 170
           RSLR DPN+D+LI+ +YP  E+YE  +D +  R       + + +SI + LR Q    + 
Sbjct: 66  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQ----AM 125

Query: 171 KRPPSIRR-LRGNNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSE 230
            R   +RR + G++     S G+         P   + +    ED   DS+ D     + 
Sbjct: 126 HRAQRVRRPIPGSDQTTTMSGGEGD-------PGEGEGD---GEDVSSDSAPDSAPGPAP 185

Query: 231 RRLKGFRTQGAA-KFSQPPLAACSDGAGEDNDSDQVHRETLGASSM 273
           +R +G    G++             GAG ++  D+    TLG  ++
Sbjct: 186 KRPRGGGAGGSSVGTGGGGTGGVGGGAGSEDSGDR--GGTLGGGTL 215

BLAST of Tan0000474 vs. NCBI nr
Match: KAG6583983.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 765.4 bits (1975), Expect = 2.7e-217
Identity = 399/443 (90.07%), Postives = 415/443 (93.68%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPA+KRAPESSEDD LKL+HGCS ARPED DADESDRS SSSSNGEKDEFIVV+LSDIRK
Sbjct: 1   MPAKKRAPESSEDDSLKLEHGCSAARPEDGDADESDRSTSSSSNGEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRGN 180
           SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPS+RRLRGN
Sbjct: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAK 240
           NY N+ S+GKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERR KGFR QGAAK
Sbjct: 181 NYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFRKQGAAK 240

Query: 241 FSQPPLAACSDGAGEDNDSDQVHRETLGASSMRVGSSERLVWGKGGMRSHTRHGSTSVGN 300
           FSQPP AACSDGAGEDNDSDQV+RE L ASSM VG+SERLVWGKGGMRSHTRHG  SVG+
Sbjct: 241 FSQPPFAACSDGAGEDNDSDQVNREILSASSMLVGNSERLVWGKGGMRSHTRHGCMSVGS 300

Query: 301 SKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLC 360
           SKNVKNNR+S LVD LR S+NNA EREV LLLFSYDQR IPSLQLPY+CCRPT SVRHLC
Sbjct: 301 SKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTSSVRHLC 360

Query: 361 QYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTLAD 420
           QYVA QTGFQADEIDM LI ELN KIEPSTSIDVLVSKSLIP P ED+FQVLRE+QTLA+
Sbjct: 361 QYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLREQQTLAE 420

Query: 421 LKTHCSTRDQLLLAYQKKPRQSR 444
           LKTHCSTR QLLLAYQKKPRQS+
Sbjct: 421 LKTHCSTRGQLLLAYQKKPRQSQ 443

BLAST of Tan0000474 vs. NCBI nr
Match: XP_022927385.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita moschata] >XP_023519096.1 putative E3 ubiquitin-protein ligase RING1a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764.6 bits (1973), Expect = 4.7e-217
Identity = 399/443 (90.07%), Postives = 414/443 (93.45%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPA+KRAPESSEDD LKLDHGCS ARPED DADESDRS SSSSNGEKDEFIVV+LSDIRK
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGCSAARPEDGDADESDRSTSSSSNGEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRGN 180
           SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPS+RRLRGN
Sbjct: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAK 240
           NY N+ S+GKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERR KGFR QGAAK
Sbjct: 181 NYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFRKQGAAK 240

Query: 241 FSQPPLAACSDGAGEDNDSDQVHRETLGASSMRVGSSERLVWGKGGMRSHTRHGSTSVGN 300
           FSQPP AACSDGAGEDNDSDQV+RE L ASS  VG+SERLVWGKGGMRSHTRHG  SVG+
Sbjct: 241 FSQPPFAACSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHGCMSVGS 300

Query: 301 SKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLC 360
           SKNVKNNR+S LVD LR S+NNA EREV LLLFSYDQR IPSLQLPY+CCRPT SVRHLC
Sbjct: 301 SKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTSSVRHLC 360

Query: 361 QYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTLAD 420
           QYVA QTGFQADEIDM LI ELN KIEPSTSIDVLVSKSLIP P ED+FQVLRE+QTLA+
Sbjct: 361 QYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLREQQTLAE 420

Query: 421 LKTHCSTRDQLLLAYQKKPRQSR 444
           LKTHCSTR QLLLAYQKKPRQS+
Sbjct: 421 LKTHCSTRGQLLLAYQKKPRQSQ 443

BLAST of Tan0000474 vs. NCBI nr
Match: XP_023001718.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita maxima])

HSP 1 Score: 756.1 bits (1951), Expect = 1.7e-214
Identity = 397/443 (89.62%), Postives = 412/443 (93.00%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPA+KRAPESSEDD LKLDHG S ARPED DADESDRS SSSSNGEKDEFIVV+LSDIRK
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGFSAARPEDGDADESDRSTSSSSNGEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRGN 180
           SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPS+RRLRGN
Sbjct: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAK 240
           NY N+ S+GKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERR KGFR QGAAK
Sbjct: 181 NYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFRKQGAAK 240

Query: 241 FSQPPLAACSDGAGEDNDSDQVHRETLGASSMRVGSSERLVWGKGGMRSHTRHGSTSVGN 300
           FSQPP AA SDGAGEDNDSDQV+RE L ASS  VG+SERLVWGKGGMRSHTRHG  SVG+
Sbjct: 241 FSQPPFAAYSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHGCMSVGS 300

Query: 301 SKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLC 360
           SKNVKNNR+S LVD LR S+NNA EREV LLLFSYDQR IPSLQLPY+CCRPT SVRHLC
Sbjct: 301 SKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTSSVRHLC 360

Query: 361 QYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTLAD 420
           QYVA QTGFQADEIDM LI ELN KIEPSTSIDVLVSKSLIP P ED+FQVLRE+QTLA+
Sbjct: 361 QYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLREQQTLAE 420

Query: 421 LKTHCSTRDQLLLAYQKKPRQSR 444
           LKTHCSTR QLLLAYQKKPRQS+
Sbjct: 421 LKTHCSTRGQLLLAYQKKPRQSQ 443

BLAST of Tan0000474 vs. NCBI nr
Match: XP_022139814.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Momordica charantia])

HSP 1 Score: 751.1 bits (1938), Expect = 5.4e-213
Identity = 394/448 (87.95%), Postives = 416/448 (92.86%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPAQKRAPESSEDDPLK DHGCS+  PED D+++SDRSPSSSSN EKDEFIVV+LSDIRK
Sbjct: 1   MPAQKRAPESSEDDPLKRDHGCSSVHPEDGDSEQSDRSPSSSSNEEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRG- 180
           SLI+ALYPDIEKYEEDELAFREEER+RNKQIQASIAQTL RQSEAVSKKRPPS+RRLRG 
Sbjct: 121 SLISALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAA 240
           NN+ NNHSRGKRSYRNDAEFP SDD EDPTAEDG KDSSSDEQ ER ERR KGFRTQGAA
Sbjct: 181 NNHRNNHSRGKRSYRNDAEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGFRTQGAA 240

Query: 241 KFSQPP-LAACSDGAGEDNDSDQVHRETLGASSMR-VGSSERLVWGKGGMRSHTRHGSTS 300
           KFSQPP LAACSDGAGEDNDS +V+RE  GA+S R VGSSERLVWGKGGMRSHTRHGS S
Sbjct: 241 KFSQPPCLAACSDGAGEDNDS-EVNREAPGAASSRHVGSSERLVWGKGGMRSHTRHGSLS 300

Query: 301 VGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVR 360
           +GNSKNVKNNRLSAL DYLRNS++N+ EREVHLLLFSYDQR IPSLQLPY+CCRP  SVR
Sbjct: 301 IGNSKNVKNNRLSALADYLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCRPASSVR 360

Query: 361 HLCQYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQT 420
           HLCQYVAHQTGFQADEIDMLLI EL+PKIEPSTSIDVLVSKSLIP PTEDNFQVLRE+QT
Sbjct: 361 HLCQYVAHQTGFQADEIDMLLIQELHPKIEPSTSIDVLVSKSLIPHPTEDNFQVLREQQT 420

Query: 421 LADLKTHCSTRDQLLLAYQKKPRQSRQG 446
           L +LKTHCSTR QLLLAYQKKPRQS+ G
Sbjct: 421 LGELKTHCSTRGQLLLAYQKKPRQSQLG 447

BLAST of Tan0000474 vs. NCBI nr
Match: XP_038894853.1 (putative E3 ubiquitin-protein ligase RING1a isoform X3 [Benincasa hispida])

HSP 1 Score: 708.0 bits (1826), Expect = 5.2e-200
Identity = 379/442 (85.75%), Postives = 397/442 (89.82%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPAQKRAPESSEDDPLKLD  CS A PEDEDADESDRSPSSSSNGEKD+FI+V+LSDIRK
Sbjct: 1   MPAQKRAPESSEDDPLKLDRDCSIACPEDEDADESDRSPSSSSNGEKDKFIIVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRGN 180
           SLIAALYPDIEKYEEDELAFREEER+RNKQIQASIAQTLRRQSEAVSKKRPPSIRRL+GN
Sbjct: 121 SLIAALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLQGN 180

Query: 181 NYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQM-ERSERRLKGFRTQGAA 240
           NY NN SRGKRSYRNDAEF ASDDNEDPT EDGVKDSSSDEQM ER ERR KGFRTQGAA
Sbjct: 181 NYRNNQSRGKRSYRNDAEFQASDDNEDPTTEDGVKDSSSDEQMAERPERRSKGFRTQGAA 240

Query: 241 KFSQPPLAACSDGAGEDNDSDQVHRETLGASSMRVGSSERLVWGKGGMRS---HTRHGST 300
           KFSQPPLAACSDGA EDNDS +V+RE  GASS       +LVWGKGGMRS   HTRHGS 
Sbjct: 241 KFSQPPLAACSDGASEDNDS-EVNREIQGASS-------KLVWGKGGMRSHTRHTRHGSM 300

Query: 301 SVGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSV 360
           SVG+SKNVKNNR+SALV+YL+ S+NN  E EV LLLFSYDQR IP+LQ PYLCCRPTL +
Sbjct: 301 SVGSSKNVKNNRISALVEYLQTSDNNCEEHEVRLLLFSYDQRIIPNLQQPYLCCRPTLLI 360

Query: 361 RHLCQYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQ 420
            HL QYVA QTGFQADEIDM LI ELNPKIEPSTSIDVLVSKSLIP P EDNFQVLR +Q
Sbjct: 361 GHLRQYVARQTGFQADEIDMFLIQELNPKIEPSTSIDVLVSKSLIPHPIEDNFQVLRGQQ 420

Query: 421 TLADLKTHCSTRDQLLLAYQKK 439
           TL++LK H STR QLLLAYQKK
Sbjct: 421 TLSELKAHRSTRGQLLLAYQKK 434

BLAST of Tan0000474 vs. ExPASy TrEMBL
Match: A0A6J1ENS0 (putative E3 ubiquitin-protein ligase RING1a OS=Cucurbita moschata OX=3662 GN=LOC111434221 PE=4 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.3e-217
Identity = 399/443 (90.07%), Postives = 414/443 (93.45%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPA+KRAPESSEDD LKLDHGCS ARPED DADESDRS SSSSNGEKDEFIVV+LSDIRK
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGCSAARPEDGDADESDRSTSSSSNGEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRGN 180
           SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPS+RRLRGN
Sbjct: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAK 240
           NY N+ S+GKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERR KGFR QGAAK
Sbjct: 181 NYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFRKQGAAK 240

Query: 241 FSQPPLAACSDGAGEDNDSDQVHRETLGASSMRVGSSERLVWGKGGMRSHTRHGSTSVGN 300
           FSQPP AACSDGAGEDNDSDQV+RE L ASS  VG+SERLVWGKGGMRSHTRHG  SVG+
Sbjct: 241 FSQPPFAACSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHGCMSVGS 300

Query: 301 SKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLC 360
           SKNVKNNR+S LVD LR S+NNA EREV LLLFSYDQR IPSLQLPY+CCRPT SVRHLC
Sbjct: 301 SKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTSSVRHLC 360

Query: 361 QYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTLAD 420
           QYVA QTGFQADEIDM LI ELN KIEPSTSIDVLVSKSLIP P ED+FQVLRE+QTLA+
Sbjct: 361 QYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLREQQTLAE 420

Query: 421 LKTHCSTRDQLLLAYQKKPRQSR 444
           LKTHCSTR QLLLAYQKKPRQS+
Sbjct: 421 LKTHCSTRGQLLLAYQKKPRQSQ 443

BLAST of Tan0000474 vs. ExPASy TrEMBL
Match: A0A6J1KRC5 (putative E3 ubiquitin-protein ligase RING1a OS=Cucurbita maxima OX=3661 GN=LOC111495770 PE=4 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 8.1e-215
Identity = 397/443 (89.62%), Postives = 412/443 (93.00%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPA+KRAPESSEDD LKLDHG S ARPED DADESDRS SSSSNGEKDEFIVV+LSDIRK
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGFSAARPEDGDADESDRSTSSSSNGEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRGN 180
           SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPS+RRLRGN
Sbjct: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAK 240
           NY N+ S+GKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERR KGFR QGAAK
Sbjct: 181 NYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFRKQGAAK 240

Query: 241 FSQPPLAACSDGAGEDNDSDQVHRETLGASSMRVGSSERLVWGKGGMRSHTRHGSTSVGN 300
           FSQPP AA SDGAGEDNDSDQV+RE L ASS  VG+SERLVWGKGGMRSHTRHG  SVG+
Sbjct: 241 FSQPPFAAYSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHGCMSVGS 300

Query: 301 SKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLC 360
           SKNVKNNR+S LVD LR S+NNA EREV LLLFSYDQR IPSLQLPY+CCRPT SVRHLC
Sbjct: 301 SKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTSSVRHLC 360

Query: 361 QYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTLAD 420
           QYVA QTGFQADEIDM LI ELN KIEPSTSIDVLVSKSLIP P ED+FQVLRE+QTLA+
Sbjct: 361 QYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLREQQTLAE 420

Query: 421 LKTHCSTRDQLLLAYQKKPRQSR 444
           LKTHCSTR QLLLAYQKKPRQS+
Sbjct: 421 LKTHCSTRGQLLLAYQKKPRQSQ 443

BLAST of Tan0000474 vs. ExPASy TrEMBL
Match: A0A6J1CE12 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010638 PE=4 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.6e-213
Identity = 394/448 (87.95%), Postives = 416/448 (92.86%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPAQKRAPESSEDDPLK DHGCS+  PED D+++SDRSPSSSSN EKDEFIVV+LSDIRK
Sbjct: 1   MPAQKRAPESSEDDPLKRDHGCSSVHPEDGDSEQSDRSPSSSSNEEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRG- 180
           SLI+ALYPDIEKYEEDELAFREEER+RNKQIQASIAQTL RQSEAVSKKRPPS+RRLRG 
Sbjct: 121 SLISALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAA 240
           NN+ NNHSRGKRSYRNDAEFP SDD EDPTAEDG KDSSSDEQ ER ERR KGFRTQGAA
Sbjct: 181 NNHRNNHSRGKRSYRNDAEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGFRTQGAA 240

Query: 241 KFSQPP-LAACSDGAGEDNDSDQVHRETLGASSMR-VGSSERLVWGKGGMRSHTRHGSTS 300
           KFSQPP LAACSDGAGEDNDS +V+RE  GA+S R VGSSERLVWGKGGMRSHTRHGS S
Sbjct: 241 KFSQPPCLAACSDGAGEDNDS-EVNREAPGAASSRHVGSSERLVWGKGGMRSHTRHGSLS 300

Query: 301 VGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVR 360
           +GNSKNVKNNRLSAL DYLRNS++N+ EREVHLLLFSYDQR IPSLQLPY+CCRP  SVR
Sbjct: 301 IGNSKNVKNNRLSALADYLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCRPASSVR 360

Query: 361 HLCQYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQT 420
           HLCQYVAHQTGFQADEIDMLLI EL+PKIEPSTSIDVLVSKSLIP PTEDNFQVLRE+QT
Sbjct: 361 HLCQYVAHQTGFQADEIDMLLIQELHPKIEPSTSIDVLVSKSLIPHPTEDNFQVLREQQT 420

Query: 421 LADLKTHCSTRDQLLLAYQKKPRQSRQG 446
           L +LKTHCSTR QLLLAYQKKPRQS+ G
Sbjct: 421 LGELKTHCSTRGQLLLAYQKKPRQSQLG 447

BLAST of Tan0000474 vs. ExPASy TrEMBL
Match: A0A6J1CDU7 (putative E3 ubiquitin-protein ligase RING1a isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010638 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 6.2e-175
Identity = 327/371 (88.14%), Postives = 344/371 (92.72%), Query Frame = 0

Query: 78  MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDE 137
           MECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLRDDPNYDSLI+ALYPDIEKYEEDE
Sbjct: 1   MECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLRDDPNYDSLISALYPDIEKYEEDE 60

Query: 138 LAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRG-NNYMNNHSRGKRSYRND 197
           LAFREEER+RNKQIQASIAQTL RQSEAVSKKRPPS+RRLRG NN+ NNHSRGKRSYRND
Sbjct: 61  LAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSVRRLRGNNNHRNNHSRGKRSYRND 120

Query: 198 AEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAKFSQPP-LAACSDGAGE 257
           AEFP SDD EDPTAEDG KDSSSDEQ ER ERR KGFRTQGAAKFSQPP LAACSDGAGE
Sbjct: 121 AEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGFRTQGAAKFSQPPCLAACSDGAGE 180

Query: 258 DNDSDQVHRETLGASSMR-VGSSERLVWGKGGMRSHTRHGSTSVGNSKNVKNNRLSALVD 317
           DNDS +V+RE  GA+S R VGSSERLVWGKGGMRSHTRHGS S+GNSKNVKNNRLSAL D
Sbjct: 181 DNDS-EVNREAPGAASSRHVGSSERLVWGKGGMRSHTRHGSLSIGNSKNVKNNRLSALAD 240

Query: 318 YLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLCQYVAHQTGFQADEI 377
           YLRNS++N+ EREVHLLLFSYDQR IPSLQLPY+CCRP  SVRHLCQYVAHQTGFQADEI
Sbjct: 241 YLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCRPASSVRHLCQYVAHQTGFQADEI 300

Query: 378 DMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTLADLKTHCSTRDQLLLA 437
           DMLLI EL+PKIEPSTSIDVLVSKSLIP PTEDNFQVLRE+QTL +LKTHCSTR QLLLA
Sbjct: 301 DMLLIQELHPKIEPSTSIDVLVSKSLIPHPTEDNFQVLREQQTLGELKTHCSTRGQLLLA 360

Query: 438 YQKKPRQSRQG 446
           YQKKPRQS+ G
Sbjct: 361 YQKKPRQSQLG 370

BLAST of Tan0000474 vs. ExPASy TrEMBL
Match: A0A6J1CDC8 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010638 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 3.5e-170
Identity = 320/373 (85.79%), Postives = 340/373 (91.15%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLDHGCSTARPEDEDADESDRSPSSSSNGEKDEFIVVRLSDIRK 60
           MPAQKRAPESSEDDPLK DHGCS+  PED D+++SDRSPSSSSN EKDEFIVV+LSDIRK
Sbjct: 1   MPAQKRAPESSEDDPLKRDHGCSSVHPEDGDSEQSDRSPSSSSNEEKDEFIVVKLSDIRK 60

Query: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 120
           EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLRDDPNYD
Sbjct: 61  EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLRDDPNYD 120

Query: 121 SLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSIRRLRG- 180
           SLI+ALYPDIEKYEEDELAFREEER+RNKQIQASIAQTL RQSEAVSKKRPPS+RRLRG 
Sbjct: 121 SLISALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSVRRLRGN 180

Query: 181 NNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAA 240
           NN+ NNHSRGKRSYRNDAEFP SDD EDPTAEDG KDSSSDEQ ER ERR KGFRTQGAA
Sbjct: 181 NNHRNNHSRGKRSYRNDAEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGFRTQGAA 240

Query: 241 KFSQPP-LAACSDGAGEDNDSDQVHRETLGASSMR-VGSSERLVWGKGGMRSHTRHGSTS 300
           KFSQPP LAACSDGAGEDNDS +V+RE  GA+S R VGSSERLVWGKGGMRSHTRHGS S
Sbjct: 241 KFSQPPCLAACSDGAGEDNDS-EVNREAPGAASSRHVGSSERLVWGKGGMRSHTRHGSLS 300

Query: 301 VGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVR 360
           +GNSKNVKNNRLSAL DYLRNS++N+ EREVHLLLFSYDQR IPSLQLPY+CCRP  SVR
Sbjct: 301 IGNSKNVKNNRLSALADYLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCRPASSVR 360

Query: 361 HLCQYVAHQTGFQ 371
           HLCQ    ++  Q
Sbjct: 361 HLCQNYTPKSSLQ 372

BLAST of Tan0000474 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 316.6 bits (810), Expect = 3.1e-86
Identity = 206/428 (48.13%), Postives = 286/428 (66.82%), Query Frame = 0

Query: 28  EDEDADESDRSPSSSSNGEKDEFIVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRE 87
           E+E+ D  +RSPSS S G++ EF+ + L +IRK+VQCPICLGII+KTRTVMECLHRFCRE
Sbjct: 101 EEEEEDSKERSPSSIS-GDQSEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRE 160

Query: 88  CIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFREEERIR 147
           CIDKSMRLGNNECPACR HCASRRSLRDDP +D+LIAAL+ +I+ YEE+ELAF E+E  R
Sbjct: 161 CIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMAR 220

Query: 148 NKQIQASIAQTLRRQSEAVSKKRP--PSIRRLRGNNYMNNHSRGKRSYRNDAEFPAS--- 207
           NKQIQASIAQ  +RQSEA+ K+R        L  +  + + SR +R+ RN  +  AS   
Sbjct: 221 NKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAH 280

Query: 208 --DDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAKFSQPPLAACSDGAGEDN--- 267
             DDN+D       KDSSSDE+   +E R K  R +  ++ +Q P    S GA ++N   
Sbjct: 281 EDDDNDDNNNRGRDKDSSSDER--GTEVRQKKRRKRSTSRSTQHP---SSSGANKNNGNC 340

Query: 268 -DSD-QVHRETLGASSMRVGSSERLVWGKGGMRSHTRHG-STSVGNSKNVKNNRLSALVD 327
            D+D +V+R+T G S   V + E L WG+GG RS+TRHG +TS G+SK+V+N R++ LV+
Sbjct: 341 ADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVE 400

Query: 328 YLRNS-NNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLCQYVAHQTGFQADE 387
           YLR+S + ++VE ++H+ L S D + IP L  PYLCCRPTL V+ L ++VA Q   + +E
Sbjct: 401 YLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEE 460

Query: 388 IDMLLIHELNPKIEPSTSIDVLVSKS-LIPRPTEDNFQVLRERQTLADLKTH--CSTRDQ 439
           +++L+      ++    +I+ L + +      ++D    L + +TL+ LK     S    
Sbjct: 461 VELLVTRR---RVGEDAAIENLPAVTPASAAASKDEMLSLEDNETLSRLKIDFISSHEQH 519

BLAST of Tan0000474 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-75
Identity = 195/459 (42.48%), Postives = 276/459 (60.13%), Query Frame = 0

Query: 3   AQKRAPES--SEDDPLKLDHGCSTARPED----------------EDADESDRSPSSSSN 62
           A++  PE+   E+DP K+D    +   ED                E+ D  +RS SSS+ 
Sbjct: 23  AERFNPEAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKERSQSSSAG 82

Query: 63  G-EKDEFIVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPAC 122
              + E++VV L+DI K+VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECP C
Sbjct: 83  ELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTC 142

Query: 123 RTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQS 182
           R HCASRRSLRDDPN+D+LIAAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS
Sbjct: 143 RKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQS 202

Query: 183 EAVSKKRP--PSIRRLRGNNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDE 242
           +A+ K++        L  +      SR +R+ RN  E   S+ N+D       KDSSSDE
Sbjct: 203 KALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDE 262

Query: 243 QMERSERRLKGFRTQGAAKFSQPPLAACSDGAGEDNDSDQVH-RETLGASSMRVGSSERL 302
             ER  +R K   TQ ++        A ++     N ++Q H R++   S + V +SE +
Sbjct: 263 PCER--QRKKRSATQPSSS------NANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELI 322

Query: 303 VWGKGGMRSHTRHGSTSVGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTI 362
            WG+GG RS+TR G+ + G + + +N RL  LV+YL +   N+VE ++HL L S D   +
Sbjct: 323 AWGRGGTRSNTRQGNNNQG-AISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGL 382

Query: 363 PSLQLPYLCCRPTLSVRHLCQYVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSL 422
            +L  PYLC RPTL V+ L +YVA     +A+E+++L+  + +  I   TS         
Sbjct: 383 LNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS--------- 442

Query: 423 IPRPTEDNFQVLRERQTLADLKTHC-STRDQLLLAYQKK 439
                 +  Q L++ +T+A LK  C S+   +++ Y++K
Sbjct: 443 -----TEKMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 457

BLAST of Tan0000474 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 274.2 bits (700), Expect = 1.8e-73
Identity = 195/465 (41.94%), Postives = 276/465 (59.35%), Query Frame = 0

Query: 3   AQKRAPES--SEDDPLKLDHGCSTARPED----------------EDADESDRSPSSSSN 62
           A++  PE+   E+DP K+D    +   ED                E+ D  +RS SSS+ 
Sbjct: 23  AERFNPEAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKERSQSSSAG 82

Query: 63  G-EKDEFIVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPAC 122
              + E++VV L+DI K+VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECP C
Sbjct: 83  ELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTC 142

Query: 123 RTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQS 182
           R HCASRRSLRDDPN+D+LIAAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS
Sbjct: 143 RKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQS 202

Query: 183 EAVSKKRP--PSIRRLRGNNYMNNHSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDE 242
           +A+ K++        L  +      SR +R+ RN  E   S+ N+D       KDSSSDE
Sbjct: 203 KALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDE 262

Query: 243 QMERSERRLKGFRTQGAAKFSQPPLAACSDGAGEDNDSDQVH-RETLGASSMRVGSSERL 302
             ER  +R K   TQ ++        A ++     N ++Q H R++   S + V +SE +
Sbjct: 263 PCER--QRKKRSATQPSSS------NANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELI 322

Query: 303 VWGKGGMRSHTRHGSTSVGNSKNVKNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTI 362
            WG+GG RS+TR G+ + G + + +N RL  LV+YL +   N+VE ++HL L S D   +
Sbjct: 323 AWGRGGTRSNTRQGNNNQG-AISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGL 382

Query: 363 PSLQLPYLCCRPTLSVRHLCQ------YVAHQTGFQADEIDMLLIHELNPKIEPSTSIDV 422
            +L  PYLC RPTL V+ L +      YVA     +A+E+++L+  + +  I   TS   
Sbjct: 383 LNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNKTS--- 442

Query: 423 LVSKSLIPRPTEDNFQVLRERQTLADLKTHC-STRDQLLLAYQKK 439
                       +  Q L++ +T+A LK  C S+   +++ Y++K
Sbjct: 443 -----------TEKMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 463

BLAST of Tan0000474 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 274.2 bits (700), Expect = 1.8e-73
Identity = 191/441 (43.31%), Postives = 269/441 (61.00%), Query Frame = 0

Query: 12  EDDPLKLDH---GCSTARPEDEDADESDRSPSSSSNG-EKDEFIVVRLSDIRKEVQCPIC 71
           E+D +K D        A  E+E +D  +RS SSS+    + E++VV L+DI K+VQC IC
Sbjct: 49  EEDDVKRDQVEAEDEEALGEEEVSDSKERSQSSSAGELSESEYMVVDLADICKDVQCSIC 108

Query: 72  LGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALY 131
           LGIIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D+LIAAL+
Sbjct: 109 LGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALF 168

Query: 132 PDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRP--PSIRRLRGNNYMNN 191
            +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS+A+ K++        L  +     
Sbjct: 169 KNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRRSGG 228

Query: 192 HSRGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAKFSQPP 251
            SR +R+ RN  E   S+ N+D       KDSSSDE  ER  +R K   TQ ++      
Sbjct: 229 GSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDEPCER--QRKKRSATQPSSS----- 288

Query: 252 LAACSDGAGEDNDSDQVH-RETLGASSMRVGSSERLVWGKGGMRSHTRHGSTSVGNSKNV 311
             A ++     N ++Q H R++   S + V +SE + WG+GG RS+TR G+ + G + + 
Sbjct: 289 -NANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGNNNQG-AISK 348

Query: 312 KNNRLSALVDYLRNSNNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLCQ--- 371
           +N RL  LV+YL +   N+VE ++HL L S D   + +L  PYLC RPTL V+ L +   
Sbjct: 349 RNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSS 408

Query: 372 ---YVAHQTGFQADEIDMLLIHELNPKIEPSTSIDVLVSKSLIPRPTEDNFQVLRERQTL 431
              YVA     +A+E+++L+  + +  I   TS               +  Q L++ +T+
Sbjct: 409 LPLYVARHLKLKAEEVELLVSKDGDTVIGNKTS--------------TEKMQSLQDDETV 465

Query: 432 ADLKTHC-STRDQLLLAYQKK 439
           A LK  C S+   +++ Y++K
Sbjct: 469 AKLKVDCISSNGYMIVVYRRK 465

BLAST of Tan0000474 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 250.0 bits (637), Expect = 3.6e-66
Identity = 182/432 (42.13%), Postives = 269/432 (62.27%), Query Frame = 0

Query: 28  EDEDADESDRSPSSSSNGEKDEFIVVRLSDIRKEVQCPICLGIIRKTRTVMECLHRFCRE 87
           E+E+ D  +RSPSS S G++ EF+ + L +IRK+VQCPICL  ++  R  +   ++  ++
Sbjct: 101 EEEEEDSKERSPSSIS-GDQSEFMEIDLGEIRKDVQCPICLVGMKFLRLFIS-YYKENKD 160

Query: 88  CIDKSMR----LGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFREE 147
           C   S +      NNECPACR HCASRRSLRDDP +D+LIAAL+ +I+ YEE+ELAF E+
Sbjct: 161 CDGMSPQFCATFKNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHED 220

Query: 148 ERIRNKQIQASIAQTLRRQSEAVSKKRP--PSIRRLRGNNYMNNHSRGKRSYRNDAEFPA 207
           E  RNKQIQASIAQ  +RQSEA+ K+R        L  +  + + SR +R+ RN  +  A
Sbjct: 221 EMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNA 280

Query: 208 S-----DDNEDPTAEDGVKDSSSDEQMERSERRLKGFRTQGAAKFSQPPLAACSDGAGED 267
           S     DDN+D       KDSSSDE+   +E R K  R +  ++ +Q P    S GA ++
Sbjct: 281 SEAHEDDDNDDNNNRGRDKDSSSDER--GTEVRQKKRRKRSTSRSTQHP---SSSGANKN 340

Query: 268 N----DSD-QVHRETLGASSMRVGSSERLVWGKGGMRSHTRHG-STSVGNSKNVKNNRLS 327
           N    D+D +V+R+T G S   V + E L WG+GG RS+TRHG +TS G+SK+V+N R++
Sbjct: 341 NGNCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVN 400

Query: 328 ALVDYLRNS-NNNAVEREVHLLLFSYDQRTIPSLQLPYLCCRPTLSVRHLCQYVAHQTGF 387
            LV+YLR+S + ++VE ++H+ L S D + IP L  PYLCCRPTL V+ L ++VA Q   
Sbjct: 401 KLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHL 460

Query: 388 QADEIDMLLIHELNPKIEPSTSIDVLVSKS-LIPRPTEDNFQVLRERQTLADLKTH--CS 439
           + +E+++L+      ++    +I+ L + +      ++D    L + +TL+ LK     S
Sbjct: 461 KTEEVELLVTRR---RVGEDAAIENLPAVTPASAAASKDEMLSLEDNETLSRLKIDFISS 520