Tan0000457 (gene) Snake gourd v1

Overview


Name	Tan0000457
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	metalloendoproteinase 2-MMP-like
Location	LG11: 5052742 .. 5054192 (+)
RNA-Seq Expression	Tan0000457
Synteny	Tan0000457

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTTGAAGGATTATCATTCTTCTCTTTCTCTTTTTTCCTTCTAATTTCCATCTTCCCTTTAATATCATCTTCTCAGCCAAACCCTGCTCATAATTCCCATGGATTGGTGTTTCTAAACAATCTCAATGGATGCAAGAAGGGTGACAAAGTGGAAGGGATCCACCAAATGAAGAAATACCTCCAACATTTTGGCTATCTCAATCATAATCATTCTAGAGCTAATGATGATGAGTTCGATGAGCTCTTAGAGTCTGCCATTAAAACTTACCAAATCAACTACAATCTCAAGGTCACAGGTACTCTCGATGCCACGACGCTAGCTCTTATGTCGAAACCTCGATGCGGGGCTTCAGATGTCATCAACGGCAAGACTGGAATGAATTCGGGCAAGAAAATAATGAACCAACATAGGAAAATAAGTGGTCATTTCCACACAGTTTCTCACTTTTCTTTCTTCCAAGGAAACCCTAAATGGCCAGCTTCCAAATTCCACCTCACTTATGGTAAATCTAATCTTTATTCATATTCTTTCATCTTTAAACTCATAATGGAGTAGTTATCAATCTTTTTCAATGAGAAAATGATATATATATTTTTTTTAAGTAAAATTGGAGTTGGATAATTCAAACTACAAATTTCGTAGTCATTAATACATTTAATACGCCAATTGAGCTATGATCTCTTTGGCATAATGAGAAAATGATATCAAGTTTGAATATTACAAGATCTTCTTTTCTAATATTGTAATTCAAAATTACAACAAAATTTATTGTGTTTGGAATAACTTGTTAAAAAAATACTTTTGGACAAAATTAATTTTTCTGTGAACACTTCGGAAAAAATTAATAATTTAAAGTGTGTTTAGTACTACTCATTGAGTGATTTTTAGATGATTAAAAGTGTTTTTTTTTAAAAAAAAAATCTTAAGAGATAATAATATATTTTTTTTAAAAGAATATTTAAACTAGAAGGTATTTGAAAGAGACTAATATTCTATTTACATCCTTAACAGGTTTCCTCCCTGGAACCCCATCAGAGGCGATAGCTCCGGTGAGTCGAGCTTTTACGACTTGGGCTGCAAACACTCACTTCACCTTCTCCCAAGTTTCTGACTATCAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGGCGATGCCTTTGATGGCATTGGAGGAGTCATAGCCCACGCCTTTTCTCCCACGGATGGGAGATTACATTTCGATGCAGTCGAGCCTTGGGCCGACGGCATTGTTCCTGATTCGTACGATGTCGAGACGGTGGCGTTGCATGAGATCGGACATCTTCTCGGCCTCCATCATAGCTCTGTGGAAGGAGCTATCATGTGGCCAACTATCATGGCCGGAGCTACCAAGGGTTTGCATGCCGACGATATTGCAGGCATTAATGCTTTATACGGTTCTACTTCTTGA

mRNA sequence

ATGGCATTTGAAGGATTATCATTCTTCTCTTTCTCTTTTTTCCTTCTAATTTCCATCTTCCCTTTAATATCATCTTCTCAGCCAAACCCTGCTCATAATTCCCATGGATTGGTGTTTCTAAACAATCTCAATGGATGCAAGAAGGGTGACAAAGTGGAAGGGATCCACCAAATGAAGAAATACCTCCAACATTTTGGCTATCTCAATCATAATCATTCTAGAGCTAATGATGATGAGTTCGATGAGCTCTTAGAGTCTGCCATTAAAACTTACCAAATCAACTACAATCTCAAGGTCACAGGTACTCTCGATGCCACGACGCTAGCTCTTATGTCGAAACCTCGATGCGGGGCTTCAGATGTCATCAACGGCAAGACTGGAATGAATTCGGGCAAGAAAATAATGAACCAACATAGGAAAATAAGTGGTCATTTCCACACAGTTTCTCACTTTTCTTTCTTCCAAGGAAACCCTAAATGGCCAGCTTCCAAATTCCACCTCACTTATGGTTTCCTCCCTGGAACCCCATCAGAGGCGATAGCTCCGGTGAGTCGAGCTTTTACGACTTGGGCTGCAAACACTCACTTCACCTTCTCCCAAGTTTCTGACTATCAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGGCGATGCCTTTGATGGCATTGGAGGAGTCATAGCCCACGCCTTTTCTCCCACGGATGGGAGATTACATTTCGATGCAGTCGAGCCTTGGGCCGACGGCATTGTTCCTGATTCGTACGATGTCGAGACGGTGGCGTTGCATGAGATCGGACATCTTCTCGGCCTCCATCATAGCTCTGTGGAAGGAGCTATCATGTGGCCAACTATCATGGCCGGAGCTACCAAGGGTTTGCATGCCGACGATATTGCAGGCATTAATGCTTTATACGGTTCTACTTCTTGA

Coding sequence (CDS)

ATGGCATTTGAAGGATTATCATTCTTCTCTTTCTCTTTTTTCCTTCTAATTTCCATCTTCCCTTTAATATCATCTTCTCAGCCAAACCCTGCTCATAATTCCCATGGATTGGTGTTTCTAAACAATCTCAATGGATGCAAGAAGGGTGACAAAGTGGAAGGGATCCACCAAATGAAGAAATACCTCCAACATTTTGGCTATCTCAATCATAATCATTCTAGAGCTAATGATGATGAGTTCGATGAGCTCTTAGAGTCTGCCATTAAAACTTACCAAATCAACTACAATCTCAAGGTCACAGGTACTCTCGATGCCACGACGCTAGCTCTTATGTCGAAACCTCGATGCGGGGCTTCAGATGTCATCAACGGCAAGACTGGAATGAATTCGGGCAAGAAAATAATGAACCAACATAGGAAAATAAGTGGTCATTTCCACACAGTTTCTCACTTTTCTTTCTTCCAAGGAAACCCTAAATGGCCAGCTTCCAAATTCCACCTCACTTATGGTTTCCTCCCTGGAACCCCATCAGAGGCGATAGCTCCGGTGAGTCGAGCTTTTACGACTTGGGCTGCAAACACTCACTTCACCTTCTCCCAAGTTTCTGACTATCAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGGCGATGCCTTTGATGGCATTGGAGGAGTCATAGCCCACGCCTTTTCTCCCACGGATGGGAGATTACATTTCGATGCAGTCGAGCCTTGGGCCGACGGCATTGTTCCTGATTCGTACGATGTCGAGACGGTGGCGTTGCATGAGATCGGACATCTTCTCGGCCTCCATCATAGCTCTGTGGAAGGAGCTATCATGTGGCCAACTATCATGGCCGGAGCTACCAAGGGTTTGCATGCCGACGATATTGCAGGCATTAATGCTTTATACGGTTCTACTTCTTGA

Protein sequence

MAFEGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPTDGRLHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLHADDIAGINALYGSTS

Homology

[Close]

BLAST of Tan0000457 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 8.8e-64
Identity = 131/283 (46.29%), Postives = 173/283 (61.13%), Query Frame = 0


Query: 42  NLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRAN-DDEFDELLESAIKTYQINYNLKVT 101
           N  GC  G K +G++ +K+Y QHFGY+   +   N  D+FD++L++A++ YQ N+ L VT
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 102 GTLDATTLALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISG-HFHTVSHFSFFQGNPK 161
           G LD  TL  +  PRCG  DV+NG + M+SG+K         G  FH V H+SFF G P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 162 WPASKFHLTYGFLP--GTPSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGD 221
           WP ++  LTY F P      E  +  SRAFT W   T  TF++V  + T+DI I F SG+
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 222 HGDGDAFDGIGGVIAHAFSPTDGRLHFDAVEPW------ADGI--VPDSYDVETVALHEI 281
           HGDG+ FDG    +AHAFSP  G  H D  E W       DG   V ++ D+E+VA+HEI
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 282 GHLLGLHHSSVEGAIMWPTIMAGATK-GLHADDIAGINALYGS 312
           GHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LYG+
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]

BLAST of Tan0000457 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-61
Identity = 136/322 (42.24%), Postives = 188/322 (58.39%), Query Frame = 0


Query: 8   FFSFSFFLLIS-----IFPLISSSQPNPAHNSHGLVF---LNNLNGCKKGDKVEGIHQMK 67
           F   S FL++S      FP  +S+   P+  +   VF    +N  GC  G  V+G++++K
Sbjct: 6   FGFLSLFLIVSPASAWFFP--NSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIK 65

Query: 68  KYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGAS 127
           KY Q FGY+    S    D+FD++L++A++ YQ N+NL VTG LDA T+  +  PRCG  
Sbjct: 66  KYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 125

Query: 128 DVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTP--S 187
           DV+NG + M+ G++   +      H H V  ++ F G P+WP ++  LTY F P  P   
Sbjct: 126 DVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTE 185

Query: 188 EAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSP 247
           E  +  SRAF  W+  T   F+    + T+DI I F +GDHGDG+ FDG+ G +AHAFSP
Sbjct: 186 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 245

Query: 248 TDGRLHFDAVEPWADGIVPDSY-------DVETVALHEIGHLLGLHHSSVEGAIMWPTIM 307
             G+ H DA E W      DS+       D+E+VA+HEIGHLLGL HSSVE +IM+PTI 
Sbjct: 246 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTIT 305

Query: 308 AGATK-GLHADDIAGINALYGS 312
            G  K  L  DD+ GI  LYG+
Sbjct: 306 TGKRKVDLTNDDVEGIQYLYGA 325

[Close]

BLAST of Tan0000457 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.8e-56
Identity = 129/282 (45.74%), Postives = 165/282 (58.51%), Query Frame = 0


Query: 41  NNLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVT 100
           + L GC  G+ + G+ ++K+Y + FGY+    +    D+FD++L+SAI TYQ N+NLKVT
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT--DDFDDVLQSAINTYQKNFNLKVT 105

Query: 101 GTLDATTLALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKW 160
           G LD++TL  + KPRCG  D+I+G + MN GK +            T   +SFF G P+W
Sbjct: 106 GKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRW 165

Query: 161 PASKFHLTYGFLP--GTPSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDH 220
           P  K  LTY F P      E     SRAFT WA  T   F++      ADI I F SG+H
Sbjct: 166 PKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEH 225

Query: 221 GDGDAFDGIGGVIAHAFSPTDGRLHFDAVEPW-------ADGIVPDS--YDVETVALHEI 280
           GDG+ FDG  G +AHA SP  G LH D  E W       +  I+P +   D+E+VA+HEI
Sbjct: 226 GDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEI 285

Query: 281 GHLLGLHHSSVEGAIMWPTIMAGATK-GLHADDIAGINALYG 311
           GHLLGL HSSVE AIM+P I  G  K  L  DDI GI  LYG
Sbjct: 286 GHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Tan0000457 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.3e-51
Identity = 121/275 (44.00%), Postives = 159/275 (57.82%), Query Frame = 0


Query: 49  GDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTL 108
           G  V G+ ++K+YL  FGY+N + S    D FD  LESAI  YQ N  L +TG LD +T+
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN-DGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTV 123

Query: 109 ALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISGHF-HTVSHFSFFQGNPKWPASKFHL 168
            LMS PRCG SD                 H  I+  F HT +H+++F G PKW  ++  L
Sbjct: 124 TLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--NRDTL 183

Query: 169 TYGF-----LPGTPSEAIAPV-SRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDG 228
           TY       L    SE +  V  RAF+ W++    +F +V D+ TAD+KI F +GDHGDG
Sbjct: 184 TYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDG 243

Query: 229 DAFDGIGGVIAHAFSPTDGRLHFDAVEPW-----ADGIVPDSYDVETVALHEIGHLLGLH 288
             FDG+ G +AHAF+P +GRLH DA E W       G    + D+E+VA HEIGHLLGL 
Sbjct: 244 LPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLG 303

Query: 289 HSSVEGAIMWPTIMAGATK-GLHADDIAGINALYG 311
           HSS E A+M+P++     K  L  DD+AG+  LYG
Sbjct: 304 HSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Tan0000457 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 2.5e-50
Identity = 121/272 (44.49%), Postives = 156/272 (57.35%), Query Frame = 0


Query: 49  GDKVEGIHQMKKYLQHFGYL-NHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATT 108
           G   +G+  +K Y  H GY+ N  H    DD FD+ L SAIKTYQ NYNL VTG  D  T
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHF---DDNFDDTLVSAIKTYQKNYNLNVTGKFDINT 107

Query: 109 LALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHL 168
           L  +  PRCG  D+I     +N+ K         +  F  +S ++FF+  P+W A    L
Sbjct: 108 LKQIMTPRCGVPDII-----INTNK---------TTSFGMISDYTFFKDMPRWQAGTTQL 167

Query: 169 TYGFLPGTPSEAI--APVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFD 228
           TY F P    +    + ++RAF+ W    +  F + + Y+TA+IKI F S +HGD   FD
Sbjct: 168 TYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFD 227

Query: 229 GIGGVIAHAFSPTDGRLHFDAVEPW-ADGIVPDS-----YDVETVALHEIGHLLGLHHSS 288
           G GG++ HAF+PTDGR HFDA E W A G V  S     +D+E+VA+HEIGHLLGL HSS
Sbjct: 228 GPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSS 287

Query: 289 VEGAIMWPTIMAGATK-GLHADDIAGINALYG 311
              AIM+P+I     K  L  DDI GI  LYG
Sbjct: 288 DLRAIMYPSIPPRTRKVNLAQDDIDGIRKLYG 302

[Close]

BLAST of Tan0000457 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 550.8 bits (1418), Expect = 7.5e-153
Identity = 271/316 (85.76%), Postives = 293/316 (92.72%), Query Frame = 0


Query: 1   MAFEGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKK 60
           MAFEGLSFF  SFFLLI IFPLISSS+PN AH SHGLVFLNNL+GCKKGDKVEGIHQMK 
Sbjct: 1   MAFEGLSFFITSFFLLIIIFPLISSSEPNNAH-SHGLVFLNNLHGCKKGDKVEGIHQMKA 60

Query: 61  YLQHFGYLNH--NHSRA-NDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCG 120
           YLQHFGYLNH  NHS++ +DDEFDE+LESAIKTYQ+NYNLK TGTLDATTLALMSKPRCG
Sbjct: 61  YLQHFGYLNHVQNHSKSFSDDEFDEVLESAIKTYQLNYNLKATGTLDATTLALMSKPRCG 120

Query: 121 ASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPS 180
            +DV++GKT M SGKK++NQHRKI+GHFH+VSH++FF+GNPKWPASK HLTYGFLPGTPS
Sbjct: 121 IADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYGFLPGTPS 180

Query: 181 EAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSP 240
           EA+APV RAFTTWAANTHF+FSQ S Y+TADIKISFESGDHGDG AFDG+GGVIAHAFSP
Sbjct: 181 EAVAPVGRAFTTWAANTHFSFSQASAYETADIKISFESGDHGDGHAFDGVGGVIAHAFSP 240

Query: 241 TDGRLHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGL 300
           TDGRLHFDAVEPWA G V DSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIM GATKGL
Sbjct: 241 TDGRLHFDAVEPWAIGAVADSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGL 300

Query: 301 HADDIAGINALYGSTS 314
           HADDIAGINALY +TS
Sbjct: 301 HADDIAGINALYTTTS 315

[Close]

BLAST of Tan0000457 vs. NCBI nr
Match: XP_023551500.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.9 bits (1361), Expect = 3.0e-146
Identity = 258/315 (81.90%), Postives = 282/315 (89.52%), Query Frame = 0


Query: 1   MAFEGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKK 60
           MA + LSFF  S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+
Sbjct: 1   MAVQSLSFFPSSLFLLITLFPLISSSHQYPAH-SHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNH--NHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGA 120
           YL  FGYLNH  NHS+  DDEFDELLESAI+TYQ+NYNL+ TGTLDA+TLALMSKPRCG 
Sbjct: 61  YLHRFGYLNHVQNHSKTYDDEFDELLESAIQTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 SDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSE 180
           +DV +GKT MNSGKK++N+HRKI G FH VSHF+FF GNPKWPASK HLTYGFLPGTP+E
Sbjct: 121 ADVFHGKTRMNSGKKMVNRHRKIGGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTE 180

Query: 181 AIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPT 240
           AIAPV RAFTTWAANTHFTFSQ S Y+TADIKISFESGDHGDG+AFDG+GGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLH 300
           DGRLHFDAVEPWA G VPDS+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM GATKGLH
Sbjct: 241 DGRLHFDAVEPWAIGAVPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 ADDIAGINALYGSTS 314
           ADDIAGINALY +TS
Sbjct: 301 ADDIAGINALYTTTS 314

[Close]

BLAST of Tan0000457 vs. NCBI nr
Match: XP_022993647.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 254/312 (81.41%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFF+ S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+YL 
Sbjct: 5   QALSFFASSLFLLITLFPLISSSPQYPAH-SHGLLFLNNLNGCKKGDTVEGLHQMKRYLC 64

Query: 64  HFGYLNH--NHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGASDV 123
            FGYL+H  NHS+  DDEFDELLESAI+TYQ+NYNL+ TGTLDA+TLALMSKPRCG +DV
Sbjct: 65  RFGYLSHVQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 INGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSEAIA 183
            +GKTGMNSGKK++N+HRKIS HFH VSHF+FF GNPKWPASK HL YGFLPGTP EAIA
Sbjct: 125 FHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIEAIA 184

Query: 184 PVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPTDGR 243
           PV RAFTTWAANTHFTFSQ S Y+TADIKISFESGDHGDG+AFDG+GGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLHADD 303
           LHFDA+E WA G VP+S+D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM GATKGLHADD
Sbjct: 245 LHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYGSTS 314
           IAGINALY +TS
Sbjct: 305 IAGINALYTATS 315

[Close]

BLAST of Tan0000457 vs. NCBI nr
Match: KAG6578618.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.9 bits (1343), Expect = 3.7e-144
Identity = 253/312 (81.09%), Postives = 280/312 (89.74%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFFS S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALSFFSSSLFLLIALFPLISSSHQYPAH-SHGLLFLNNLNGCKKGDMVEGLHQMKRYLR 64

Query: 64  HFGYLN--HNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGASDV 123
            FGYLN   NHS+  DDEFDE+LESAI+TYQ+NYNL+ TGTLDA+TLALM KPRCG +DV
Sbjct: 65  RFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMLKPRCGVADV 124

Query: 124 INGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSEAIA 183
            +GKT MNSGKK++N+HRKISG FH VSHF+FF GNPKWPASK HLTYGFLPGTP+EAIA
Sbjct: 125 FHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIA 184

Query: 184 PVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPTDGR 243
           PV RAFTTWAANTHFTFS  S Y+TADIKISFESGDHGDG+AFDG+GGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLHADD 303
           LHFDAVEPWA G +PDS+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM GATKGLHADD
Sbjct: 245 LHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYGSTS 314
           IAGINALY +TS
Sbjct: 305 IAGINALYTTTS 315

[Close]

BLAST of Tan0000457 vs. NCBI nr
Match: KAG7016165.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520.0 bits (1338), Expect = 1.4e-143
Identity = 252/312 (80.77%), Postives = 280/312 (89.74%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFF+ S FLLI++FPLISSS   PA  SHGL+FLNNLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALSFFASSLFLLIALFPLISSSHQYPA-QSHGLLFLNNLNGCKKGDTVEGLHQMKRYLR 64

Query: 64  HFGYLN--HNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGASDV 123
            FGYLN   NHS+  DDEFDE+LESAI+TYQ+NYNL+ TGTLDA+TLALMSKPRCG +DV
Sbjct: 65  RFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 INGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSEAIA 183
            +GKT MNSGKK++N+HRKISG FH VSHF+FF GNPKWPASK HLTYGFLPGTP+EAIA
Sbjct: 125 FHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIA 184

Query: 184 PVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPTDGR 243
           PV RAFTTWAANTHFTFS  S Y+TADIKISFESGDHGDG+AFDG+GGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLHADD 303
           LHFDAVEPWA G +PDS+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM GATKGLHADD
Sbjct: 245 LHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYGSTS 314
           IAGINALY +TS
Sbjct: 305 IAGINALYTTTS 315

[Close]

BLAST of Tan0000457 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.2e-145
Identity = 254/312 (81.41%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFF+ S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+YL 
Sbjct: 5   QALSFFASSLFLLITLFPLISSSPQYPAH-SHGLLFLNNLNGCKKGDTVEGLHQMKRYLC 64

Query: 64  HFGYLNH--NHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGASDV 123
            FGYL+H  NHS+  DDEFDELLESAI+TYQ+NYNL+ TGTLDA+TLALMSKPRCG +DV
Sbjct: 65  RFGYLSHVQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 INGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSEAIA 183
            +GKTGMNSGKK++N+HRKIS HFH VSHF+FF GNPKWPASK HL YGFLPGTP EAIA
Sbjct: 125 FHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIEAIA 184

Query: 184 PVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPTDGR 243
           PV RAFTTWAANTHFTFSQ S Y+TADIKISFESGDHGDG+AFDG+GGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLHADD 303
           LHFDA+E WA G VP+S+D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM GATKGLHADD
Sbjct: 245 LHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYGSTS 314
           IAGINALY +TS
Sbjct: 305 IAGINALYTATS 315

[Close]

BLAST of Tan0000457 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.4e-143
Identity = 251/312 (80.45%), Postives = 279/312 (89.42%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSF + S FLLI++FPLISSS   PA  SHGL+FLNNLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALSFLASSLFLLIALFPLISSSHQYPA-QSHGLLFLNNLNGCKKGDTVEGLHQMKRYLR 64

Query: 64  HFGYLN--HNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGASDV 123
            FGYLN   NHS+  DDEFDE+LESAI+TYQ+NYNL+ TGTLDA+TLALMSKPRCG +DV
Sbjct: 65  RFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 INGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTPSEAIA 183
            +GKT MNSGKK++N+HRKISG FH VSHF+FF GNPKWPASK HLTYGFLPGTP+EAIA
Sbjct: 125 FHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIA 184

Query: 184 PVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSPTDGR 243
           PV RAFTTWAANTHFTFS  S Y+TADIKISFESGDHGDG+AFDG+GGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKGLHADD 303
           LHFDAVEPWA G +PDS+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM GATKGLHADD
Sbjct: 245 LHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYGSTS 314
           IAGINALY +TS
Sbjct: 305 IAGINALYTTTS 315

[Close]

BLAST of Tan0000457 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 9.6e-138
Identity = 244/313 (77.96%), Postives = 270/313 (86.26%), Query Frame = 0


Query: 1   MAFEGLS-FFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMK 60
           M F+GLS FF+F+ FL+IS+FPLISSSQP P     GLVFL NL GCKKGDKVEGIHQMK
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPV---DGLVFLKNLKGCKKGDKVEGIHQMK 60

Query: 61  KYLQHFGYLN---HNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRC 120
           KYLQHFGYLN   H  S ++DDEFDE+LE A+KTYQINYNLKVTGTLD +TL  MSKPRC
Sbjct: 61  KYLQHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTP 180
           G +D+INGKT M SGK+ +NQHRKISGHFH VSHF+FF+GN +WP SK HLTYGFLPGTP
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP 180

Query: 181 SEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFS 240
           SEAI+PV+RAFTTWAANTHF+FSQ S Y+ ADIKISFE GDHGDG  FD +GGV+AHAF+
Sbjct: 181 SEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFA 240

Query: 241 PTDGRLHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATKG 300
           PTDGRLHFDAVE WADG VP SYD+ETVALHEIGHLLGLHHSSVEGAIMWP+IM GATKG
Sbjct: 241 PTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKG 300

Query: 301 LHADDIAGINALY 310
           LHADDI GI  LY
Sbjct: 301 LHADDIEGIKVLY 310

[Close]

BLAST of Tan0000457 vs. ExPASy TrEMBL
Match: A0A1S3C9I6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.4e-134
Identity = 238/316 (75.32%), Postives = 274/316 (86.71%), Query Frame = 0


Query: 1   MAFEG-LSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMK 60
           M FE  LSF + SFFL+I +FPLISSS PN  H+S+GL FL NLNGC+KGDKV GIHQ+K
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPN-IHHSNGLAFLKNLNGCRKGDKVAGIHQIK 60

Query: 61  KYLQHFGYLNH---NHSRA-NDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPR 120
           +YLQHFGYLN+   N+S++ +DDEFD +LESAIKTYQ+NYNLK TGTLDA TL LMSKPR
Sbjct: 61  EYLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPR 120

Query: 121 CGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGT 180
           CG +D+I+GKT M SGKK++NQHRKI+GHFHTVSH++FF GNPKWPASKFHLTYGFLPGT
Sbjct: 121 CGVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGT 180

Query: 181 PSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAF 240
           PS+A+APV RAF TW A+THF FS+ S Y+ ADIKISF SGDHGDG +FDG+GGVIAH F
Sbjct: 181 PSKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGF 240

Query: 241 SPTDGRLHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATK 300
           +PTDGRLHFDA+E WA G V DS+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM G TK
Sbjct: 241 APTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTK 300

Query: 301 GLHADDIAGINALYGS 312
           GL ADDIAGINALY +
Sbjct: 301 GLDADDIAGINALYNA 315

[Close]

BLAST of Tan0000457 vs. ExPASy TrEMBL
Match: A0A5D3BMC5 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00180 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.4e-133
Identity = 237/316 (75.00%), Postives = 273/316 (86.39%), Query Frame = 0


Query: 1   MAFEG-LSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMK 60
           M FE  LSF + SFFL+I +FPLISSS PN  H+S+GL FL NLNGC+KGDKV GIHQ+K
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPN-IHHSNGLAFLKNLNGCRKGDKVAGIHQIK 60

Query: 61  KYLQHFGYLNH---NHSRA-NDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPR 120
           +YLQHFGYLN+   N+S++ +DDEFD +LESAIKTYQ+NYNLK TGTLDA TL LMSKPR
Sbjct: 61  EYLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPR 120

Query: 121 CGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGT 180
           CG +D+I+GKT M SGKK++NQHRKI+GHFHTVSH++FF GNPKWPASK HLTYGFLPGT
Sbjct: 121 CGVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGT 180

Query: 181 PSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAF 240
           PS+A+APV RAF TW A+THF FS+ S Y+ ADIKISF SGDHGDG +FDG+GGVIAH F
Sbjct: 181 PSKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGF 240

Query: 241 SPTDGRLHFDAVEPWADGIVPDSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMAGATK 300
           +PTDGRLHFDA+E WA G V DS+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM G TK
Sbjct: 241 APTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTK 300

Query: 301 GLHADDIAGINALYGS 312
           GL ADDIAGINALY +
Sbjct: 301 GLDADDIAGINALYNA 315

[Close]

BLAST of Tan0000457 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.3e-65
Identity = 131/283 (46.29%), Postives = 173/283 (61.13%), Query Frame = 0


Query: 42  NLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRAN-DDEFDELLESAIKTYQINYNLKVT 101
           N  GC  G K +G++ +K+Y QHFGY+   +   N  D+FD++L++A++ YQ N+ L VT
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 102 GTLDATTLALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISG-HFHTVSHFSFFQGNPK 161
           G LD  TL  +  PRCG  DV+NG + M+SG+K         G  FH V H+SFF G P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 162 WPASKFHLTYGFLP--GTPSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGD 221
           WP ++  LTY F P      E  +  SRAFT W   T  TF++V  + T+DI I F SG+
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 222 HGDGDAFDGIGGVIAHAFSPTDGRLHFDAVEPW------ADGI--VPDSYDVETVALHEI 281
           HGDG+ FDG    +AHAFSP  G  H D  E W       DG   V ++ D+E+VA+HEI
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 282 GHLLGLHHSSVEGAIMWPTIMAGATK-GLHADDIAGINALYGS 312
           GHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LYG+
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]

BLAST of Tan0000457 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 238.0 bits (606), Expect = 1.0e-62
Identity = 136/322 (42.24%), Postives = 188/322 (58.39%), Query Frame = 0


Query: 8   FFSFSFFLLIS-----IFPLISSSQPNPAHNSHGLVF---LNNLNGCKKGDKVEGIHQMK 67
           F   S FL++S      FP  +S+   P+  +   VF    +N  GC  G  V+G++++K
Sbjct: 6   FGFLSLFLIVSPASAWFFP--NSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIK 65

Query: 68  KYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKPRCGAS 127
           KY Q FGY+    S    D+FD++L++A++ YQ N+NL VTG LDA T+  +  PRCG  
Sbjct: 66  KYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 125

Query: 128 DVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPASKFHLTYGFLPGTP--S 187
           DV+NG + M+ G++   +      H H V  ++ F G P+WP ++  LTY F P  P   
Sbjct: 126 DVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTE 185

Query: 188 EAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGIGGVIAHAFSP 247
           E  +  SRAF  W+  T   F+    + T+DI I F +GDHGDG+ FDG+ G +AHAFSP
Sbjct: 186 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 245

Query: 248 TDGRLHFDAVEPWADGIVPDSY-------DVETVALHEIGHLLGLHHSSVEGAIMWPTIM 307
             G+ H DA E W      DS+       D+E+VA+HEIGHLLGL HSSVE +IM+PTI 
Sbjct: 246 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTIT 305

Query: 308 AGATK-GLHADDIAGINALYGS 312
            G  K  L  DD+ GI  LYG+
Sbjct: 306 TGKRKVDLTNDDVEGIQYLYGA 325

[Close]

BLAST of Tan0000457 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-57
Identity = 129/282 (45.74%), Postives = 165/282 (58.51%), Query Frame = 0


Query: 41  NNLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVT 100
           + L GC  G+ + G+ ++K+Y + FGY+    +    D+FD++L+SAI TYQ N+NLKVT
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT--DDFDDVLQSAINTYQKNFNLKVT 105

Query: 101 GTLDATTLALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKW 160
           G LD++TL  + KPRCG  D+I+G + MN GK +            T   +SFF G P+W
Sbjct: 106 GKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRW 165

Query: 161 PASKFHLTYGFLP--GTPSEAIAPVSRAFTTWAANTHFTFSQVSDYQTADIKISFESGDH 220
           P  K  LTY F P      E     SRAFT WA  T   F++      ADI I F SG+H
Sbjct: 166 PKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEH 225

Query: 221 GDGDAFDGIGGVIAHAFSPTDGRLHFDAVEPW-------ADGIVPDS--YDVETVALHEI 280
           GDG+ FDG  G +AHA SP  G LH D  E W       +  I+P +   D+E+VA+HEI
Sbjct: 226 GDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEI 285

Query: 281 GHLLGLHHSSVEGAIMWPTIMAGATK-GLHADDIAGINALYG 311
           GHLLGL HSSVE AIM+P I  G  K  L  DDI GI  LYG
Sbjct: 286 GHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Tan0000457 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 204.9 bits (520), Expect = 9.4e-53
Identity = 121/275 (44.00%), Postives = 159/275 (57.82%), Query Frame = 0


Query: 49  GDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTL 108
           G  V G+ ++K+YL  FGY+N + S    D FD  LESAI  YQ N  L +TG LD +T+
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN-DGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTV 123

Query: 109 ALMSKPRCGASDVINGKTGMNSGKKIMNQHRKISGHF-HTVSHFSFFQGNPKWPASKFHL 168
            LMS PRCG SD                 H  I+  F HT +H+++F G PKW  ++  L
Sbjct: 124 TLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--NRDTL 183

Query: 169 TYGF-----LPGTPSEAIAPV-SRAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDG 228
           TY       L    SE +  V  RAF+ W++    +F +V D+ TAD+KI F +GDHGDG
Sbjct: 184 TYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDG 243

Query: 229 DAFDGIGGVIAHAFSPTDGRLHFDAVEPW-----ADGIVPDSYDVETVALHEIGHLLGLH 288
             FDG+ G +AHAF+P +GRLH DA E W       G    + D+E+VA HEIGHLLGL 
Sbjct: 244 LPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLG 303

Query: 289 HSSVEGAIMWPTIMAGATK-GLHADDIAGINALYG 311
           HSS E A+M+P++     K  L  DD+AG+  LYG
Sbjct: 304 HSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Tan0000457 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.2e-44
Identity = 106/269 (39.41%), Postives = 146/269 (54.28%), Query Frame = 0


Query: 55  IHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKVTGTLDATTLALMSKP 114
           I ++K++LQ +GYL       N +  D   E A+  YQ N  L +TG  D+ TL+ +  P
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLP 109

Query: 115 RCGASDVINGKTGMNSGKKIMNQHRKISGHFHTVSHFSFFQGNPKWPAS-KFHLTYGFLP 174
           RCG  D +  KT                  FHT   + +F G P+W       LTY F  
Sbjct: 110 RCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQ 169

Query: 175 GTPSEAIAPVS------RAFTTWAANTHFTFSQVSDYQTADIKISFESGDHGDGDAFDGI 234
              +  +AP        RAF  WA+    +F +  DY  ADIKI F +GDHGDG+ FDG+
Sbjct: 170 ENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGV 229

Query: 235 GGVIAHAFSPTDGRLHFDAVEPWADGIVPD----SYDVETVALHEIGHLLGLHHSSVEGA 294
            GV+AH FSP +GRLH D  E WA     +    + D+E+VA+HEIGH+LGL HSSV+ A
Sbjct: 230 LGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDA 289

Query: 295 IMWPTIMAGATK-GLHADDIAGINALYGS 312
            M+PT+   + K  L+ DD+ G+ +LYG+
Sbjct: 290 AMYPTLKPRSKKVNLNMDDVVGVQSLYGT 297

The following BLAST results are available for this feature:

BLAST of Tan0000457 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	8.8e-64	46.29	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	1.4e-61	42.24	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	1.8e-56	45.74	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O23507	1.3e-51	44.00	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
P29136	2.5e-50	44.49	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]

BLAST of Tan0000457 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889209.1	7.5e-153	85.76	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_023551500.1	3.0e-146	81.90	metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993647.1	1.3e-144	81.41	metalloendoproteinase 2-MMP-like [Cucurbita maxima]	[more]
KAG6578618.1	3.7e-144	81.09	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7016165.1	1.4e-143	80.77	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of Tan0000457 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTF5	6.2e-145	81.41	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=...	[more]
A0A6J1FDT9	3.4e-143	80.45	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P...	[more]
A0A0A0KS47	9.6e-138	77.96	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S...	[more]
A0A1S3C9I6	6.4e-134	75.32	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV...	[more]
A0A5D3BMC5	5.4e-133	75.00	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Tan0000457 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	6.3e-65	46.29	Matrixin family protein	[more]
AT1G70170.1	1.0e-62	42.24	matrix metalloproteinase	[more]
AT1G59970.1	1.3e-57	45.74	Matrixin family protein	[more]
AT4G16640.1	9.4e-53	44.00	Matrixin family protein	[more]
AT2G45040.1	1.2e-44	39.41	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 183..198 score: 34.91 coord: 264..289 score: 57.69 coord: 297..310 score: 60.19 coord: 111..124 score: 50.17 coord: 207..235 score: 58.37
IPR021190	Peptidase M10A	PIRSF	PIRSF001191	MMP_stromelysin	coord: 23..313 e-value: 8.1E-62 score: 207.3
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 156..311 e-value: 7.5E-32 score: 121.8
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 164..310 e-value: 1.3E-42 score: 145.4
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 53..111 e-value: 8.5E-13 score: 48.3
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 46..313 e-value: 8.9E-80 score: 269.7
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 13..310
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 13..310
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 153..310
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 159..310 e-value: 3.08903E-57 score: 180.093
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 57..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0000457.1	Tan0000457.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity