Tan0000447 (gene) Snake gourd v1

Overview


Name	Tan0000447
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	LG05: 71656743 .. 71658115 (+)
RNA-Seq Expression	Tan0000447
Synteny	Tan0000447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACCCAATATTTGCAAAACACAACAAAATTTCTTTCTTATTTTGTGAGCTCATTTTTGTGTCCAACAATGGCGGATCAGATTCCAGTGGTGAAATTTTCTTCAGAGAATTTGAAGAGTGGAACAAGGAACTGGATTTTGACGAGGGAGAAAGTTCGAGAAGCTCTTGAAGAATATGGGTGCTTTGTAGCTTTATACGACAACGTTTCGATGGAACTGAGTTCCAATATGTTCGATTCCTTAAAAGAGTTGTTCGATGTTCCATTAGAGAGGAAATTTCAGAATGTTTCTGAAAAACCCTACCATGGCTACTTTGGCCAAAACCCTCTAATGCCCATTCATGAAAGTATGGGCATTGAAGACCCAATTCTCTTAACCAATATTCAATCTTTCACTAATCTCATGTGGCCCTCTAATGGAAACAAACTTTTTTGGTAACTTCATCTCTCTCGAGTTATAAGTTAGTATTTATTTGATCTTGTATTTTAAAAAGTTTTTAATATCTAAAACGTTTATAAAATAGACGAACTTTAATATCATACTTGGAAATTTAATGGAAAACAATGTTTTTTTTCCGAAAATCTTAACTAGTATATGAATATGAAACTAACTATTTTCATCATCTTAAAAAAATGCAGTGAAAATGTCAACTCCTATGCAAAATTGGTATCGCAGTTAGACCAAACAGTGAAAAATATGGTCTTTGAAAGCTATGGCGTGAGAGATCACTTAGATTCTCACGTGAAATCTACGAGATATCTTTTGCGAATGATCAAATACAGAGTTCCACAACAAAACGAGATGAATTTGGGAGCATTTCCTCACACAGACAAGAGCTTCCTCACTATTCTTCACCAAAATCAAGTGAATGGCTTGCAAATCAAAACCAAAGATAACAAATGGCTTGAATATGAGCCTTCTTATTCTTGTTTATCTTCTACCTCATCTTTTATTGTCATGGCCGGAGATGCCTTCTTTGTAAGTTTATTAATTAACCTTCAAATTCTCTCATATGTAAAAGATTAAAATTATCATAATTAATTATAGCTATCGAACTTAACATAAAGTTTGGTCCTTTGTTGGCACAGGCATGGAGCAATGGAAGGATTTACTCACCACCTCATAGAGTGATGATGAATGGGAAGAGAGAAAGATATTCAATGGGGTTGTTTTCATTTAATGATGGGATTGTTCAGATTCCTAAAGAGTTGGTGGATGAAACTCATCCACAACAATTTAAGGCATTTGATCATCAAGATTATCTTCGTTTTTATAGCACTGAAAGAGGTCAAAATTCTCCTTGTGCTATTAAAGCTTATTGTGGTGTTGGAAATGCCCTCCTTGATACTAGGGGTTGCATTCTCTTGCAGTAA

mRNA sequence

AAAACCCAATATTTGCAAAACACAACAAAATTTCTTTCTTATTTTGTGAGCTCATTTTTGTGTCCAACAATGGCGGATCAGATTCCAGTGGTGAAATTTTCTTCAGAGAATTTGAAGAGTGGAACAAGGAACTGGATTTTGACGAGGGAGAAAGTTCGAGAAGCTCTTGAAGAATATGGGTGCTTTGTAGCTTTATACGACAACGTTTCGATGGAACTGAGTTCCAATATGTTCGATTCCTTAAAAGAGTTGTTCGATGTTCCATTAGAGAGGAAATTTCAGAATGTTTCTGAAAAACCCTACCATGGCTACTTTGGCCAAAACCCTCTAATGCCCATTCATGAAAGTATGGGCATTGAAGACCCAATTCTCTTAACCAATATTCAATCTTTCACTAATCTCATGTGGCCCTCTAATGGAAACAAACTTTTTTGTGAAAATGTCAACTCCTATGCAAAATTGGTATCGCAGTTAGACCAAACAGTGAAAAATATGGTCTTTGAAAGCTATGGCGTGAGAGATCACTTAGATTCTCACGTGAAATCTACGAGATATCTTTTGCGAATGATCAAATACAGAGTTCCACAACAAAACGAGATGAATTTGGGAGCATTTCCTCACACAGACAAGAGCTTCCTCACTATTCTTCACCAAAATCAAGTGAATGGCTTGCAAATCAAAACCAAAGATAACAAATGGCTTGAATATGAGCCTTCTTATTCTTGTTTATCTTCTACCTCATCTTTTATTGTCATGGCCGGAGATGCCTTCTTTGCATGGAGCAATGGAAGGATTTACTCACCACCTCATAGAGTGATGATGAATGGGAAGAGAGAAAGATATTCAATGGGGTTGTTTTCATTTAATGATGGGATTGTTCAGATTCCTAAAGAGTTGGTGGATGAAACTCATCCACAACAATTTAAGGCATTTGATCATCAAGATTATCTTCGTTTTTATAGCACTGAAAGAGGTCAAAATTCTCCTTGTGCTATTAAAGCTTATTGTGGTGTTGGAAATGCCCTCCTTGATACTAGGGGTTGCATTCTCTTGCAGTAA

Coding sequence (CDS)

ATGGCGGATCAGATTCCAGTGGTGAAATTTTCTTCAGAGAATTTGAAGAGTGGAACAAGGAACTGGATTTTGACGAGGGAGAAAGTTCGAGAAGCTCTTGAAGAATATGGGTGCTTTGTAGCTTTATACGACAACGTTTCGATGGAACTGAGTTCCAATATGTTCGATTCCTTAAAAGAGTTGTTCGATGTTCCATTAGAGAGGAAATTTCAGAATGTTTCTGAAAAACCCTACCATGGCTACTTTGGCCAAAACCCTCTAATGCCCATTCATGAAAGTATGGGCATTGAAGACCCAATTCTCTTAACCAATATTCAATCTTTCACTAATCTCATGTGGCCCTCTAATGGAAACAAACTTTTTTGTGAAAATGTCAACTCCTATGCAAAATTGGTATCGCAGTTAGACCAAACAGTGAAAAATATGGTCTTTGAAAGCTATGGCGTGAGAGATCACTTAGATTCTCACGTGAAATCTACGAGATATCTTTTGCGAATGATCAAATACAGAGTTCCACAACAAAACGAGATGAATTTGGGAGCATTTCCTCACACAGACAAGAGCTTCCTCACTATTCTTCACCAAAATCAAGTGAATGGCTTGCAAATCAAAACCAAAGATAACAAATGGCTTGAATATGAGCCTTCTTATTCTTGTTTATCTTCTACCTCATCTTTTATTGTCATGGCCGGAGATGCCTTCTTTGCATGGAGCAATGGAAGGATTTACTCACCACCTCATAGAGTGATGATGAATGGGAAGAGAGAAAGATATTCAATGGGGTTGTTTTCATTTAATGATGGGATTGTTCAGATTCCTAAAGAGTTGGTGGATGAAACTCATCCACAACAATTTAAGGCATTTGATCATCAAGATTATCTTCGTTTTTATAGCACTGAAAGAGGTCAAAATTCTCCTTGTGCTATTAAAGCTTATTGTGGTGTTGGAAATGCCCTCCTTGATACTAGGGGTTGCATTCTCTTGCAGTAA

Protein sequence

MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFDVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTERGQNSPCAIKAYCGVGNALLDTRGCILLQ

Homology

[Close]

BLAST of Tan0000447 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-76
Identity = 135/316 (42.72%), Postives = 210/316 (66.46%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+PV+ FS +NLK G+  W   +  V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +P+  K +NVS KP+HGY   N    ++ES+GI+D  +L  +  FT  +WP +GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVP-----QQNEMN 183
            ++ +++ + +LD  V+ M+ ES+G+ +++D H+ ST YL R++KY  P        E  
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEETK 192

Query: 184 LGAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWS 243
           LG   HTDK+ +TILHQ QV+GL++KTKD+KW++ +P      S  S +VM GD+  A  
Sbjct: 193 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP------SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSPPHRVMMNGKRERYSMGLFSF--NDGIVQIPKELVDETHPQQFKAFDHQDYLRF 303
           NGR++SP HRV+M GK+ RYS GLFS      I+  P+ELVD+ HP+ FK F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTERGQNSPCAIKAY 313
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

[Close]

BLAST of Tan0000447 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-75
Identity = 135/317 (42.59%), Postives = 208/317 (65.62%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+PV+ FS +NLK G+  W      V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +P+  K +NVS KP+HGY   N    ++ES+GI D  +L  +  FT  +WP +GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVP------QQNEM 183
            ++ +++ + +LD  V+ M+ ES+G+ +++D H+ ST YL R++KY  P         E 
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAW 243
            LG   HTDK+ +TILHQ QV+GL++KTKD+KW++ +P      S  S +VM GD+  A 
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP------SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSPPHRVMMNGKRERYSMGLFSF--NDGIVQIPKELVDETHPQQFKAFDHQDYLR 303
            NGR++SP HRV+M GK+ RYS GLFS      I+  P+ELVD+ HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTERGQNSPCAIKAY 313
           F+ TE G+ +  A+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of Tan0000447 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 132/306 (43.14%), Postives = 201/306 (65.69%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+PV+ FS +NLK G+  W      V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +P+  K +NVS K +HGY   N    ++ES+GI D  +L  +  FT  +WP +GNK   E
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 125

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVP----QQNEMNL 183
            ++ +++ + +LD  V+ M+ ES+G+  ++D H+ ST YL R++KY  P       E  L
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETKL 185

Query: 184 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 243
           G   HTDK+ +TILHQ QV+GL++KTKD+KW++ +P      S  S +VM GD+  A  N
Sbjct: 186 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP------SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSPPHRVMMNGKRERYSMGLFSF--NDGIVQIPKELVDETHPQQFKAFDHQDYLRFY 303
           GR++SP HRV+M GK+ RYS GLFS      I+  P+ELVD+ HP+ FK F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

[Close]

BLAST of Tan0000447 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.8e-66
Identity = 130/309 (42.07%), Postives = 185/309 (59.87%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           ++PV+  S +NLK GT +W   R +VREALEEYGCF A+ D VS EL   + +   EL +
Sbjct: 8   RLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLN 67

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +PLE K  N ++  Y G+     +  ++E MG+     L  ++ FTNLMWP  GNK FCE
Sbjct: 68  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCE 127

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAFP 183
            V SY K ++++D  +K MVFES+G+  H DS  +ST YLL  ++Y+ P ++  +     
Sbjct: 128 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 187

Query: 184 HTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIY 243
           H DKS LTI++QN V GL+ +TKD +W+        L +  + IV+ GD F AWSNGR++
Sbjct: 188 HKDKSILTIVNQNDVKGLEFETKDGEWI--------LPTADNHIVLLGDCFMAWSNGRLH 247

Query: 244 SPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTERGQ 303
           SP HRV +   + R S   FSF   I++ P ELVDE HP  F  F+  + L +  T+ G 
Sbjct: 248 SPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGA 304

Query: 304 NSPCAIKAY 313
            + C +K Y
Sbjct: 308 KAVCDLKQY 304

[Close]

BLAST of Tan0000447 vs. ExPASy Swiss-Prot
Match: Q9ZTA1 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.9e-58
Identity = 135/411 (32.85%), Postives = 197/411 (47.93%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+P++  S + LK G+  W+  R  VR+ALE+YGCF A  D VSMEL  ++  +++ELF 
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQGSVLKAMQELFA 66

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +P E K +NV  KP+ GY   N L    ES GI+D  +L     FT  +WP  GNK   +
Sbjct: 67  LPTEAKQRNVCPKPFTGYLSHNGL---SESFGIKDANILEKAHEFTQQLWP-EGNKSISK 126

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEM------ 183
            +  YA+ +++LD  V+ ++ ESYG+   +D H+ ST Y +R++KY     N++      
Sbjct: 127 MIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVGA 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 NVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDLD 246

Query: 244 ------------------------------NLGAFPHTDKSFLTILHQNQVNGLQIKTKD 303
                                          LG   HTDKS   I++Q+Q++GL++KTK+
Sbjct: 247 AEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGLEVKTKE 306

Query: 304 NKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIYSPPHRVMMNGKRE-RYSMGLFSF- 316
            KW+  +P      + ++ IV+AGDA  A  NGRI SP HRV +  K++ RY+  LFS  
Sbjct: 307 GKWIRVKP------APNTVIVIAGDALCALMNGRIPSPYHRVRVTEKKKTRYAAALFSNP 366

[Close]

BLAST of Tan0000447 vs. NCBI nr
Match: XP_022137575.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Momordica charantia])

HSP 1 Score: 587.8 bits (1514), Expect = 5.8e-164
Identity = 279/325 (85.85%), Postives = 303/325 (93.23%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+P+VKFS ENL    R+W+ TREKVREALEEYGCFVALYD+VSMELSSNMFDSLKELF+
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFE 64

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           VPLERK +NVSEKPYHGYFGQNPLMPIHESMGIEDP LL+  QSFTNLMWPSNGNK FCE
Sbjct: 65  VPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFCE 124

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAFP 183
           NVNSYA+ +++LD+TVK MVFESYGVR HL+SH+KSTRYLLRMIKYRVP+QNEMN+GAFP
Sbjct: 125 NVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAFP 184

Query: 184 HTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIY 243
           HTDKSFLTILHQNQVNGLQIKTK NKWL+Y PS S  SSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 185 HTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPS-SSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 244 SPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTERGQ 303
           SPPH+V+M+GKRERYS+GLFSFNDGIVQIPKELVDE HPQQFK FDHQDYLRFYSTERGQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 304 NSPCAIKAYCGVGNALLDTRGCILL 329
           NSPCAIKAYCG+GN L+DTRGCILL
Sbjct: 305 NSPCAIKAYCGIGNGLVDTRGCILL 324

[Close]

BLAST of Tan0000447 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 555.1 bits (1429), Expect = 4.2e-154
Identity = 263/330 (79.70%), Postives = 296/330 (89.70%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKE 60
           M  +IP+VKF SENLKSG + W+LTREKVREALEEYGCFVALYD VSM++SSNMF SLK+
Sbjct: 1   MGGEIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQ 60

Query: 61  LFDVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKL 120
           LFDVPLERK QNVSEKPYHGYFGQNPLMPIHESMGIE PIL TNI SFTNLMWPS+GN+ 
Sbjct: 61  LFDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRP 120

Query: 121 FCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLG 180
           FCENV  YAKLVS+LD+ VK+MVFESYGV   L +H++ST+YL+RMIKYRVP++ EMNLG
Sbjct: 121 FCENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLG 180

Query: 181 AFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNG 240
           AFPHTDKSFLTILHQN+VNGLQIKT+DNKWL+Y P  S  SSTSSFIVMAGDAFFAWSNG
Sbjct: 181 AFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSS--SSTSSFIVMAGDAFFAWSNG 240

Query: 241 RIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTE 300
           RIYSPPHRV+++  +ERYS+GLFSFNDGI+QIPKELVD+ HPQQF  FDHQDYLRFY+TE
Sbjct: 241 RIYSPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTE 300

Query: 301 RGQNSPCAIKAYCGVGNALLDT--RGCILL 329
           RGQNSPCAIKAYCGVGN+L+DT  R  +LL
Sbjct: 301 RGQNSPCAIKAYCGVGNSLVDTDSRSTLLL 328

[Close]

BLAST of Tan0000447 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 554.7 bits (1428), Expect = 5.4e-154
Identity = 263/327 (80.43%), Postives = 295/327 (90.21%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           QIP+VKF SENLKSG + W+LTREKVREALEEYGCFVALYD VSM++SSNMF SLK+LFD
Sbjct: 5   QIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLFD 64

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           VPLERK QNVSEKPYHGYFGQNPLMPIHESMGIE PIL TNI SFTNLMWPS+GN+ FCE
Sbjct: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFCE 124

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAFP 183
           NV  YAKLVS+LD+ VK+MVFESYGV   L +H++ST+YL+RMIKYRVP++ EMNLGAFP
Sbjct: 125 NVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAFP 184

Query: 184 HTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIY 243
           HTDKSFLTILHQN+VNGLQIKT+DNKWL+Y P  S  SSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 185 HTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSS--SSTSSFIVMAGDAFFAWSNGRIY 244

Query: 244 SPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTERGQ 303
           SPPHRV+++  +ERYS+GLFSFNDGI+QIPKELVD+ HPQQF  FDHQDYLRFY+TERGQ
Sbjct: 245 SPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQ 304

Query: 304 NSPCAIKAYCGVGNALLDT--RGCILL 329
           NSPCAIKAYCGVGN+L+DT  R  +LL
Sbjct: 305 NSPCAIKAYCGVGNSLVDTDSRSTLLL 329

[Close]

BLAST of Tan0000447 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 544.3 bits (1401), Expect = 7.4e-151
Identity = 262/331 (79.15%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKE 60
           M+D++PVVKF SENLKSGT+ W+ T +KVREALE+YGCFVALYD+VS E+SSNMFDSLKE
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKE 64

Query: 61  LF-DVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNK 120
           LF DVPLERK QNVSEKPYHGYFGQNPLMPIHESMGIE PIL TNI SFTNLMWPS GN 
Sbjct: 65  LFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGND 124

Query: 121 LFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNL 180
            F E+V +YAKLVS+LD+ VK MVFESYGV + L SH++ST+YL+RMIKYRVP++ EMNL
Sbjct: 125 SFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNL 184

Query: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 240
           GAFPHTDKSFLTILHQN+VNGLQIKT+DNKW++Y P  S  SSTSSFIVMAGDAFFAWSN
Sbjct: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS--SSTSSFIVMAGDAFFAWSN 244

Query: 241 GRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST 300
           GRIYSPPHRV+M+G RERYS+GLFSFN+GIVQIP+ELVD+ HPQ F  FDHQD+LRFYST
Sbjct: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYST 304

Query: 301 ERGQNSPCAIKAYCGVGNAL--LDTRGCILL 329
           ERGQNSPCAIKAYCGVGN+L  +DTR  +LL
Sbjct: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPLLL 333

[Close]

BLAST of Tan0000447 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 543.9 bits (1400), Expect = 9.6e-151
Identity = 260/321 (81.00%), Postives = 289/321 (90.03%), Query Frame = 0


Query: 3   DQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELF 62
           +Q+PVVKF S+NLKSGT  W+ T +KVREALEEYGCFVALYD VS E+SSNMFDSLKELF
Sbjct: 11  EQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELF 70

Query: 63  D-VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLF 122
           D VPLERK QNVSEKPYHGYFGQNPLMPIHESMGIE PIL TNI SFTNLMWPS GN  F
Sbjct: 71  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 130

Query: 123 CENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGA 182
            ENV +YA LVS+LD+ VK+MVFESYGV + L SH++ST+YL+RMIKYRVP++ EMNLGA
Sbjct: 131 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 190

Query: 183 FPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGR 242
           FPHTDKSFLTILHQN+VNGLQIKT+DNKW++Y P  S  SSTSSFIVMAGDAFFAWSNGR
Sbjct: 191 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS--SSTSSFIVMAGDAFFAWSNGR 250

Query: 243 IYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTER 302
           IYSPPHRV+M+G +ERYS+GLFSFN+GIVQIPKELVD+ HPQ FK FDHQDYLRFYSTER
Sbjct: 251 IYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTER 310

Query: 303 GQNSPCAIKAYCGVGNALLDT 323
           GQNSPCAIKAYCGVGN+L+DT
Sbjct: 311 GQNSPCAIKAYCGVGNSLIDT 329

[Close]

BLAST of Tan0000447 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.8e-164
Identity = 279/325 (85.85%), Postives = 303/325 (93.23%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+P+VKFS ENL    R+W+ TREKVREALEEYGCFVALYD+VSMELSSNMFDSLKELF+
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFE 64

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           VPLERK +NVSEKPYHGYFGQNPLMPIHESMGIEDP LL+  QSFTNLMWPSNGNK FCE
Sbjct: 65  VPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFCE 124

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAFP 183
           NVNSYA+ +++LD+TVK MVFESYGVR HL+SH+KSTRYLLRMIKYRVP+QNEMN+GAFP
Sbjct: 125 NVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAFP 184

Query: 184 HTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIY 243
           HTDKSFLTILHQNQVNGLQIKTK NKWL+Y PS S  SSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 185 HTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPS-SSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 244 SPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTERGQ 303
           SPPH+V+M+GKRERYS+GLFSFNDGIVQIPKELVDE HPQQFK FDHQDYLRFYSTERGQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 304 NSPCAIKAYCGVGNALLDTRGCILL 329
           NSPCAIKAYCG+GN L+DTRGCILL
Sbjct: 305 NSPCAIKAYCGIGNGLVDTRGCILL 324

[Close]

BLAST of Tan0000447 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.6e-151
Identity = 262/331 (79.15%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKE 60
           M+D++PVVKF SENLKSGT+ W+ T +KVREALE+YGCFVALYD+VS E+SSNMFDSLKE
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKE 64

Query: 61  LF-DVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNK 120
           LF DVPLERK QNVSEKPYHGYFGQNPLMPIHESMGIE PIL TNI SFTNLMWPS GN 
Sbjct: 65  LFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGND 124

Query: 121 LFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNL 180
            F E+V +YAKLVS+LD+ VK MVFESYGV + L SH++ST+YL+RMIKYRVP++ EMNL
Sbjct: 125 SFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNL 184

Query: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 240
           GAFPHTDKSFLTILHQN+VNGLQIKT+DNKW++Y P  S  SSTSSFIVMAGDAFFAWSN
Sbjct: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS--SSTSSFIVMAGDAFFAWSN 244

Query: 241 GRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST 300
           GRIYSPPHRV+M+G RERYS+GLFSFN+GIVQIP+ELVD+ HPQ F  FDHQD+LRFYST
Sbjct: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYST 304

Query: 301 ERGQNSPCAIKAYCGVGNAL--LDTRGCILL 329
           ERGQNSPCAIKAYCGVGN+L  +DTR  +LL
Sbjct: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPLLL 333

[Close]

BLAST of Tan0000447 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.3e-148
Identity = 257/327 (78.59%), Postives = 288/327 (88.07%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSS-NMFDSLK 60
           MA +IPVV+FSS NL+SG R W  TREKVREALEEYGCFVALYD+VSME SS  M +++K
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  ELFDVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNK 120
           E+F+VPLERK QNVSEKP+HGYFGQNPLMPIHESMGIEDP+L +NIQSF+NLMWPS  N 
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNL 180
           LFCEN+ SYA+LVSQL+QTVK MVFESYG++    SH +ST+YLLRMIKYRVP +NEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 240
           GAFPHTDKSFLTILHQN VNGL IKT+DN+W+EY+P  S  SS  SFIVMAGDAFFAWSN
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPP-SAESSAKSFIVMAGDAFFAWSN 240

Query: 241 GRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST 300
           GRIYS PHRV M+G RERYS+GLFSFNDGIVQIPKELVD+ HPQQFKAFDHQDYLRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 301 ERGQNSPCAIKAYCGVGNALLDTRGCI 327
           E+GQ+SP AIKAYCGV N LLD+R CI
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCI 326

[Close]

BLAST of Tan0000447 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 9.1e-147
Identity = 253/327 (77.37%), Postives = 287/327 (87.77%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSS-NMFDSLK 60
           MA ++PVV+FSS NL+SG R W  TREKVREALEEYGCFVAL D+VSME SS  M ++++
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  ELFDVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNK 120
           E+F+VPLERK QNVSEKP+HGYFGQNPLMPIHESMGIEDP+L +NIQSF+NLMWPS  N 
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNL 180
           LFCEN+ SYA+LVSQL+QTVK MVFESYG++    SH +ST+YLLRMIKYRVP +NEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 240
           GAFPHTDKSFLTILHQN VNGL IKT+DN+W+EY+P  S  SS  SFI+MAGDAFFAWSN
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPP-SAESSAKSFIIMAGDAFFAWSN 240

Query: 241 GRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST 300
           GRIYS PHRV M+G RERYS+GLFSFNDGIVQIPKELVD+ HPQQFKAFDHQDYLRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 301 ERGQNSPCAIKAYCGVGNALLDTRGCI 327
           E+GQ+SP AIKAYCGV N LLD+R CI
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCI 326

[Close]

BLAST of Tan0000447 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.5e-146
Identity = 253/327 (77.37%), Postives = 286/327 (87.46%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSS-NMFDSLK 60
           MA +IPVV+FSS N++SG R W  TREKVREALEEYGCFVAL D+VSME SS  M +++K
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  ELFDVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNK 120
           E+F+VPLERK QNVS+KP+HGYFGQNPLMPIHESMGIEDP+L +NIQSF+NLMWPS  N 
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNL 180
           LFC N+ SYA+LVSQL+QTVK MVFESYG++    SH +ST+YLLRMIKYRVP +NEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 240
           GAFPHTDKSFLTILHQN VNGL IKT+DN+W+EY+P  S  SS  SFIVMAGDAFFAWSN
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPP-SAQSSARSFIVMAGDAFFAWSN 240

Query: 241 GRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST 300
           GRIYS PHRV M+G RERYS+GLFSFNDGIVQIPKELVD+ HPQQFKAFDHQDYLRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 301 ERGQNSPCAIKAYCGVGNALLDTRGCI 327
           E+GQ+SP AIKAYCGV N LLD+R CI
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCI 326

[Close]

BLAST of Tan0000447 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 327.0 bits (837), Expect = 1.7e-89
Identity = 154/315 (48.89%), Postives = 217/315 (68.89%), Query Frame = 0


Query: 1   MADQIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVA-LYDNVSMELSSNMFDSLK 60
           M+ +IP + FS E+LK GT+ W  TRE +R+ALEEYGCF+  L D   ++L   +F SL 
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLV 60

Query: 61  ELFDVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNK 120
           +LFD+P + K +N  +KP +GY GQ P +P+HES+GI++   L   +SFT LMWP  GN+
Sbjct: 61  DLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNE 120

Query: 121 LFCENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNL 180
            F E +  YA+  ++LDQ V  MVF+SY V  + D +++ST YLLR++K R P      L
Sbjct: 121 HFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSN 240
           G   HTDKSF TILHQ+QVNGL+++T++ + +        LSS S F+V+AGDA  AWSN
Sbjct: 181 GFVTHTDKSFTTILHQDQVNGLEMETREGERININ-----LSSPSLFMVVAGDALMAWSN 240

Query: 241 GRIYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST 300
            R++SP H+V+++G+ +RYS+G+F+FN+G +Q+P+EL+D  HP  +K FDH   LRFY T
Sbjct: 241 DRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRT 300

Query: 301 ERGQNSPCAIKAYCG 315
           + G  S C IKAYCG
Sbjct: 301 DTGYKSECPIKAYCG 309

[Close]

BLAST of Tan0000447 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.2e-88
Identity = 149/314 (47.45%), Postives = 216/314 (68.79%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           ++PV+ FS++NLK G   W LTR  V++AL++YG F A +D +  EL  ++F +L+ELFD
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +PL+ K +NVS+KP+HGY GQ P++P++ESMGI+D  +   + +FT  +WP  GN  F  
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 130

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAFP 183
            + S++K +S+LD T++ M+ ES+G+  ++D H+ ST YLLR++KY+ P   E  +G   
Sbjct: 131 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 190

Query: 184 HTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGRIY 243
           HTDK+ +TIL+QN V GL+++TKD  W++ +P      +  SF VM GD+ +A  NGR++
Sbjct: 191 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP------TQDSFTVMIGDSLYALLNGRLH 250

Query: 244 SPPHRVMMNGKRERYSMGLFSFNDG--IVQIPKELVDETHPQQFKAFDHQDYLRFYSTER 303
           SP HRVMM G   RYS+GLFS      IV  P ELVDE HP+ FK FDH ++L+FY TE 
Sbjct: 251 SPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEA 310

Query: 304 GQNSPCAIKAYCGV 316
           GQ S  A+K YCG+
Sbjct: 311 GQRSQSALKTYCGI 317

[Close]

BLAST of Tan0000447 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 7.0e-83
Identity = 145/314 (46.18%), Postives = 211/314 (67.20%), Query Frame = 0


Query: 4   QIPVVKFSS-ENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELF 63
           ++P++  +S + LK  T  W     +  EALEEYGCF+A+YD V+ +L  ++F + +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 64  DVPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFC 123
           D+P E K +NV+EKPYHGY GQ P++P+HE +G++        Q FT+LMWP  GN  FC
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFC 128

Query: 124 ENVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGAF 183
             V++++  V++LD+ V  M+FE+YGV  H +SHV S  YLL+ +KY  P ++ +++ AF
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPAF 188

Query: 184 P-HTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGR 243
           P HTDK+FL+ILHQN VNGL++K+KD +W+      S      S++VMAGD    WSN R
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGEWI------SLQLPPKSYVVMAGDISMGWSNDR 248

Query: 244 IYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYSTER 303
           I S  HRV M G + RY++GLFSF   +V IP+ELVD+ HP  +K FD+   + FY+T+ 
Sbjct: 249 IRSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKE 308

Query: 304 GQNSPCAIKAYCGV 316
           G+ +   +KAYCG+
Sbjct: 309 GREANSTLKAYCGI 314

[Close]

BLAST of Tan0000447 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 7.5e-77
Identity = 135/317 (42.59%), Postives = 208/317 (65.62%), Query Frame = 0


Query: 4   QIPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFD 63
           Q+PV+ FS +NLK G+  W      V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 64  VPLERKFQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 123
           +P+  K +NVS KP+HGY   N    ++ES+GI D  +L  +  FT  +WP +GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 124 NVNSYAKLVSQLDQTVKNMVFESYGVRDHLDSHVKSTRYLLRMIKYRVP------QQNEM 183
            ++ +++ + +LD  V+ M+ ES+G+ +++D H+ ST YL R++KY  P         E 
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAW 243
            LG   HTDK+ +TILHQ QV+GL++KTKD+KW++ +P      S  S +VM GD+  A 
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP------SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSPPHRVMMNGKRERYSMGLFSF--NDGIVQIPKELVDETHPQQFKAFDHQDYLR 303
            NGR++SP HRV+M GK+ RYS GLFS      I+  P+ELVD+ HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTERGQNSPCAIKAY 313
           F+ TE G+ +  A+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of Tan0000447 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.2e-70
Identity = 134/317 (42.27%), Postives = 195/317 (61.51%), Query Frame = 0


Query: 5   IPVVKFSSENLKSGTRNWILTREKVREALEEYGCFVALYDNVSMELSSNMFDSLKELFDV 64
           +P++ FSS+ L  GT +WI TR+ VR A+E  G FVA +  VS +L  N+   +KE++ +
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 65  PLERKFQNVSEKPYHGYFGQ-NPLMPIHESMGIEDPILLTNIQSFTNLMWPSNGNKLFCE 124
           P + K +N + K  HGY         IHES+GI+    L   + F+ L+WP  GN  FC+
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 125 NVNSYAKLVSQLDQTVKNMVFESYGV--RDHLDSHVKSTRYLLRMIKYRVPQQNEMNLGA 184
             + YA  +++LDQTV  M++ESYG+  + H  SH +STRYLLRM+ YR  Q  E N G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 185 FPHTDKSFLTILHQNQVNGLQIKTKDNKWLEYEPSYSCLSSTSSFIVMAGDAFFAWSNGR 244
             HTDKSF++ILHQN V GLQ+KT   +W+ + P      S + F+V++G    AWSN R
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP------SPTRFVVLSGMGLTAWSNDR 248

Query: 245 IYSPPHRVMMNGKRERYSMGLFSFNDGIVQIPKELVDETHPQQFKAFDHQDYLRFYST-- 304
           I +  H+V+M+    RYS+G FSF+ G ++ P+ELVD+ HP ++  F+H   LRFY +  
Sbjct: 249 IKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYI 308

Query: 305 -ERGQNSPCAIKAYCGV 316
               ++S   ++ YCGV
Sbjct: 309 NSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of Tan0000447 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	1.2e-76	42.72	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	1.1e-75	42.59	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	1.7e-73	43.14	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	5.8e-66	42.07	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q9ZTA1	9.9e-58	32.85	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Tan0000447 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137575.1	5.8e-164	85.85	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Momordica charant...	[more]
XP_038895877.1	4.2e-154	79.70	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_038893580.1	5.4e-154	80.43	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
TYJ99087.1	7.4e-151	79.15	putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa]	[more]
XP_004151475.3	9.6e-151	81.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570...	[more]

BLAST of Tan0000447 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C712	2.8e-164	85.85	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara...	[more]
A0A5D3BIZ7	3.6e-151	79.15	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1EAT7	1.3e-148	78.59	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1E7C8	9.1e-147	77.37	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KFF7	4.5e-146	77.37	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Tan0000447 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	1.7e-89	48.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	4.2e-88	47.45	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	7.0e-83	46.18	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	7.5e-77	42.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	5.2e-70	42.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 169..265 e-value: 2.7E-19 score: 69.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 5..83 e-value: 1.0E-8 score: 35.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..296 e-value: 2.8E-57 score: 196.7
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 3..314
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 3..314
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..302
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 158..268 score: 11.309815

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0000447.1	Tan0000447.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding