Tan0000332 (gene) Snake gourd v1

Overview


Name	Tan0000332
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	rRNA N-glycosidase
Location	LG06: 29837230 .. 29838069 (+)
RNA-Seq Expression	Tan0000332
Synteny	Tan0000332

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGATTCTTAGTCCTCTCTCTGCTTATTCTCACCATCTTCCTTGAAGCTGTTTCAACAGTCGAAGGCGATGTGAGCTTCAGTTTATCAGGAGGAACTGCTTCGTACGAGAAGTTCATCGAAGATCTGAGAAAGGCTCTTCCATCTAAGGAGTTAGTATACAAGATACCACTATTACTTCCCTCCGCTTCGGGCATAAATCGGTACACCCTAATGAAACTCTCCAATTATGACGGCAAAGCCATCACAATGGCTATCGATGTAACAAACGTTTACATTATGGGGTATCTTGTGAATACAACATCCTACTTTTTCAACGAGTCTGATGCTAAATCAGCCTCTCAATATGTATTCAAAGGTAGTACAGTTTTTACACTTCCATATTCTGGCAATTACGAAAGGCTTCAAATTGCTGCAGGTAAAAAGAGAGAGATTATTCCCCTCGGATTCCAAGCTTTGGACAGCGCCATTACCACTTTGTTTCATTACGACTCCACTTCTGCTGCTGGGGCACTTCTCGTAATCATTCAGACCACTGCTGAGGCTTCAAGATATAAATATATTGAGCAACAAATTCTTGAGAGAGCTTACAAAGACGAGGCTCCAAGTTTAGCAACTATAAGTTTAGAAAATAGCTGGTCTGCTCTCTCCAAACAAATTCAGTTAGCGCAAACTAATAATGGAAATTTTAAAACTTCTGTTGTCATTACAGACAATAAAGGCAGACCAGTCGAAATTAAAAACGTTAGTTCAAAAGTTGTCACCAACAACATCAAGTTGCTTCTTAATAAACAAAATGTTGTAGGTTTTGATGACTATGTTTCTACAAAACACTGA

mRNA sequence

ATGAAAAGATTCTTAGTCCTCTCTCTGCTTATTCTCACCATCTTCCTTGAAGCTGTTTCAACAGTCGAAGGCGATGTGAGCTTCAGTTTATCAGGAGGAACTGCTTCGTACGAGAAGTTCATCGAAGATCTGAGAAAGGCTCTTCCATCTAAGGAGTTAGTATACAAGATACCACTATTACTTCCCTCCGCTTCGGGCATAAATCGGTACACCCTAATGAAACTCTCCAATTATGACGGCAAAGCCATCACAATGGCTATCGATGTAACAAACGTTTACATTATGGGGTATCTTGTGAATACAACATCCTACTTTTTCAACGAGTCTGATGCTAAATCAGCCTCTCAATATGTATTCAAAGGTAGTACAGTTTTTACACTTCCATATTCTGGCAATTACGAAAGGCTTCAAATTGCTGCAGGTAAAAAGAGAGAGATTATTCCCCTCGGATTCCAAGCTTTGGACAGCGCCATTACCACTTTGTTTCATTACGACTCCACTTCTGCTGCTGGGGCACTTCTCGTAATCATTCAGACCACTGCTGAGGCTTCAAGATATAAATATATTGAGCAACAAATTCTTGAGAGAGCTTACAAAGACGAGGCTCCAAGTTTAGCAACTATAAGTTTAGAAAATAGCTGGTCTGCTCTCTCCAAACAAATTCAGTTAGCGCAAACTAATAATGGAAATTTTAAAACTTCTGTTGTCATTACAGACAATAAAGGCAGACCAGTCGAAATTAAAAACGTTAGTTCAAAAGTTGTCACCAACAACATCAAGTTGCTTCTTAATAAACAAAATGTTGTAGGTTTTGATGACTATGTTTCTACAAAACACTGA

Coding sequence (CDS)

ATGAAAAGATTCTTAGTCCTCTCTCTGCTTATTCTCACCATCTTCCTTGAAGCTGTTTCAACAGTCGAAGGCGATGTGAGCTTCAGTTTATCAGGAGGAACTGCTTCGTACGAGAAGTTCATCGAAGATCTGAGAAAGGCTCTTCCATCTAAGGAGTTAGTATACAAGATACCACTATTACTTCCCTCCGCTTCGGGCATAAATCGGTACACCCTAATGAAACTCTCCAATTATGACGGCAAAGCCATCACAATGGCTATCGATGTAACAAACGTTTACATTATGGGGTATCTTGTGAATACAACATCCTACTTTTTCAACGAGTCTGATGCTAAATCAGCCTCTCAATATGTATTCAAAGGTAGTACAGTTTTTACACTTCCATATTCTGGCAATTACGAAAGGCTTCAAATTGCTGCAGGTAAAAAGAGAGAGATTATTCCCCTCGGATTCCAAGCTTTGGACAGCGCCATTACCACTTTGTTTCATTACGACTCCACTTCTGCTGCTGGGGCACTTCTCGTAATCATTCAGACCACTGCTGAGGCTTCAAGATATAAATATATTGAGCAACAAATTCTTGAGAGAGCTTACAAAGACGAGGCTCCAAGTTTAGCAACTATAAGTTTAGAAAATAGCTGGTCTGCTCTCTCCAAACAAATTCAGTTAGCGCAAACTAATAATGGAAATTTTAAAACTTCTGTTGTCATTACAGACAATAAAGGCAGACCAGTCGAAATTAAAAACGTTAGTTCAAAAGTTGTCACCAACAACATCAAGTTGCTTCTTAATAAACAAAATGTTGTAGGTTTTGATGACTATGTTTCTACAAAACACTGA

Protein sequence

MKRFLVLSLLILTIFLEAVSTVEGDVSFSLSGGTASYEKFIEDLRKALPSKELVYKIPLLLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVFKGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQTTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVITDNKGRPVEIKNVSSKVVTNNIKLLLNKQNVVGFDDYVSTKH

Homology

[Close]

BLAST of Tan0000332 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 5.7e-107
Identity = 218/281 (77.58%), Postives = 237/281 (84.34%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEGDVSFSLSGGTA-SYEKFIEDLRKALPSKELVYKIPL 60
           MKRF V   LIL IF+ A STVE DV FSLSG ++ SY KFI DLRKALPS   VY I L
Sbjct: 1   MKRFTV---LILAIFV-AASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITL 60

Query: 61  LLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVF 120
           LL SASG +RYTLM LSNYDGKAIT+A+DVTNVYIMGYLVN+TSYFFNESDAK ASQYVF
Sbjct: 61  LLSSASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQT 180
           KGST+ TLPYSGNYE+LQ AAGK RE IPLGF ALDSAITTLFHYDST+AA A LVIIQT
Sbjct: 121 KGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQT 180

Query: 181 TAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVITD 240
           TAEASR+KYIE QI+ER  K++ PSLATISLEN WSALSKQIQLAQTNNG FKT VVITD
Sbjct: 181 TAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITD 240

Query: 241 NKGRPVEIKNVSSKVVTNNIKLLLN-KQNVVGFDDYVSTKH 280
           +KG+ VEI NV+SKVVT NI+LLLN KQNV  FD+ VS KH
Sbjct: 241 DKGQRVEITNVTSKVVTKNIQLLLNYKQNVAAFDEDVSAKH 277

[Close]

BLAST of Tan0000332 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 354.8 bits (909), Expect = 9.2e-97
Identity = 199/282 (70.57%), Postives = 225/282 (79.79%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEGDVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPL 60
           M RF VLS LIL IFL   S V+GDVSF LSG    SY  FI+DLR ALP +E VY IPL
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVF 120
           LLPS SG  RY LM L NYDGK IT+A+DVTNVYIMGYL +TTSYFFNE  A+ ASQYVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KGS-TVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQ 180
           + +    TLPYSGNYERLQIAAGK RE IP+G  ALDSAI+TL HYDST+AAGALLV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 TTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVIT 240
           TTAEA+R+KYIEQQI ERAY+DE PSLATISLENSWS LSKQIQLAQ NNG F+T +V+ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNKGRPVEIKNVSSKVVTNNIKLLLNKQNVV-GFDDYVSTKH 280
           DNKG  V+I NV+SKVVT+NI+LLLN +N+  G +  VST H
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTTH 281

[Close]

BLAST of Tan0000332 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 346.7 bits (888), Expect = 2.5e-94
Identity = 190/241 (78.84%), Postives = 204/241 (84.65%), Query Frame = 0


Query: 25  DVSFSLSGGTA-SYEKFIEDLRKALPSKELVYKIPLLLPSASGINRYTLMKLSNYDGKAI 84
           DVSFSLSG ++ SY KFI  LRKALPS   VY I LLL SASG +RYTLMKLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVFKGSTVFTLPYSGNYERLQIAAGKK 144
           T+AIDVTNVYIMGYLVN+TSYFFNESDAK ASQYVF GST+ TLPYSGNYE+LQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 REIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQTTAEASRYKYIEQQILERAYKDEAP 204
           RE IPLGF ALDSAITTLFHYDST+AA A LVIIQTTAE+SR+KYIE QI+ R  K+  P
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLATISLENSWSALSKQIQLAQTNNGNFKTSVVITDNKGRPVEIKNVSSKVVTNNIKLLL 264
           SLATISLEN WSALSKQIQLAQTNNG FKT VVI D  G+ VEI NV SKVVT NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

[Close]

BLAST of Tan0000332 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 3.4e-91
Identity = 186/248 (75.00%), Postives = 209/248 (84.27%), Query Frame = 0


Query: 25  DVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPLLLPSASGINRYTLMKLSNYDGKAI 84
           +VSFSLSG  + SY KFI  LRKALPSKE V  IPLLLPSASG +RY LM+LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVFKGSTVFTLPYSGNYERLQIAAGKK 144
           TMAIDVTNVYIMGYLVN+TSYF NESDAK ASQYVFKGST+ T+PYSGNYERLQ AAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 REIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQTTAEASRYKYIEQQILERAYKDEAP 204
           RE IPLGF+ALDSA+T++FHYDST+AA A LVI+QTTAEASR+KYIE QI+ER  K+E P
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLATISLEN-SWSALSKQIQLAQTNNGNFKTSVVITDNKGRPVEIKNVSSKVVTN--NIK 264
           S A +SLEN +WS LSKQIQLAQTNNG F+T VVI DNKG+ VEI N++SKV     N K
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNKQNV 269
           LLLNKQN+
Sbjct: 242 LLLNKQNI 249

[Close]

BLAST of Tan0000332 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.8e-84
Identity = 169/278 (60.79%), Postives = 208/278 (74.82%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEGDVS--FSLSGGT-ASYEKFIEDLRKALPSKELVYKI 60
           M RF VL  L++      V T EGDV+  FSL G    SY KFI  +R ALP+   +Y I
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  PLLLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQY 120
           PLL+PS SG  RY LM+LSNY+G  ITMA+DVTNVYIMGYLVN TSYFFNE+DA+ AS++
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFKGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVII 180
           VF+G+   TLPYSGNY++LQ  A K+R+ IPLGF ALDSAI+TL++YDS SA  A LV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVI 240
           QTTAEA+RYKYIE+QI++R    + P LA ISLEN WS LSKQIQ+A++NNG F+T V I
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 TDNKGRPVEIKNVSSKVVTNNIKLLLNKQNVVGFDDYV 276
            ++KG   E+ NVSS VVT NI LLLNK N+  F+D+V
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIASFEDHV 278

[Close]

BLAST of Tan0000332 vs. NCBI nr
Match: XP_023513713.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 437.6 bits (1124), Expect = 8.2e-119
Identity = 232/280 (82.86%), Postives = 252/280 (90.00%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLE---AVSTVEGDVSFSLSGGTASYEKFIEDLRKALPSKELVYKI 60
           MKR  +LS LILT+FL      S+V+ DVSFSLSGG  SYEKFIEDLRK LPS E+VYKI
Sbjct: 10  MKRLSMLSSLILTVFLTIFLGASSVQSDVSFSLSGGPTSYEKFIEDLRKTLPSNEMVYKI 69

Query: 61  PLLLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQY 120
           PLL  SASG++RYTLMKL+NYDGK+IT AIDVTNVYIMGYLVNTTSYFFNE++AK ASQY
Sbjct: 70  PLLRASASGVSRYTLMKLTNYDGKSITAAIDVTNVYIMGYLVNTTSYFFNETEAKLASQY 129

Query: 121 VFKGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVII 180
           VFKGST+FTLPYSGNYERLQIAAGKKRE IPLGF+ALDSAITTLFHYDST+AAGALLVII
Sbjct: 130 VFKGSTIFTLPYSGNYERLQIAAGKKREAIPLGFRALDSAITTLFHYDSTAAAGALLVII 189

Query: 181 QTTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVI 240
           QTTAEASRYKYIEQQILERAYK+EAPS A ISLENSWSALSKQIQLAQ+N GNFK  VVI
Sbjct: 190 QTTAEASRYKYIEQQILERAYKNEAPSPAAISLENSWSALSKQIQLAQSNGGNFKNPVVI 249

Query: 241 TDNKGRPVEIKNVSSKVVTNNIKLLLNKQNVVGFDDYVST 278
           TDNKG+PV+I NVSSKVVTNNIKLLLNK N+V FD+ VST
Sbjct: 250 TDNKGKPVQINNVSSKVVTNNIKLLLNKINIVAFDNVVST 289

[Close]

BLAST of Tan0000332 vs. NCBI nr
Match: ABY71834.1 (ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aegyptiaca])

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-104
Identity = 217/281 (77.22%), Postives = 237/281 (84.34%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEG-DVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIP 60
           M RF  LSLLI+ I   A  TVEG +VSFSLSG  + SY KFI  LRKALPSKE V  IP
Sbjct: 1   MNRFTFLSLLIILI---AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIP 60

Query: 61  LLLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYV 120
           LLLPSASG +RY LM+LSNYD KAITMAIDVTNVYIMGYLVN+TSYFFNESDAK ASQYV
Sbjct: 61  LLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYV 120

Query: 121 FKGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQ 180
           FKGST+ TLPYSGNYERLQ AAGK RE IPLGF+A DSAIT+LFHYDST+AAGA LVIIQ
Sbjct: 121 FKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQ 180

Query: 181 TTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVIT 240
           TTAEASR+KYIE QI+ER  K+E PS A +SLEN WSALSKQIQLAQTNNG F+T VVI 
Sbjct: 181 TTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVII 240

Query: 241 DNKGRPVEIKNVSSKVVTNNIKLLLNKQNVVGFDDYVSTKH 280
           DNKG+ VEIK+V+SKVVTNNIKLLLNKQN+  FDD + TKH
Sbjct: 241 DNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 278

[Close]

BLAST of Tan0000332 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 388.7 bits (997), Expect = 4.4e-104
Identity = 218/281 (77.58%), Postives = 237/281 (84.34%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEGDVSFSLSGGTA-SYEKFIEDLRKALPSKELVYKIPL 60
           MKRF V   LIL IF+ A STVE DV FSLSG ++ SY KFI DLRKALPS   VY I L
Sbjct: 1   MKRFTV---LILAIFV-AASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITL 60

Query: 61  LLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVF 120
           LL SASG +RYTLM LSNYDGKAIT+A+DVTNVYIMGYLVN+TSYFFNESDAK ASQYVF
Sbjct: 61  LLSSASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQT 180
           KGST+ TLPYSGNYE+LQ AAGK RE IPLGF ALDSAITTLFHYDST+AA A LVIIQT
Sbjct: 121 KGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQT 180

Query: 181 TAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVITD 240
           TAEASR+KYIE QI+ER  K++ PSLATISLEN WSALSKQIQLAQTNNG FKT VVITD
Sbjct: 181 TAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITD 240

Query: 241 NKGRPVEIKNVSSKVVTNNIKLLLN-KQNVVGFDDYVSTKH 280
           +KG+ VEI NV+SKVVT NI+LLLN KQNV  FD+ VS KH
Sbjct: 241 DKGQRVEITNVTSKVVTKNIQLLLNYKQNVAAFDEDVSAKH 277

[Close]

BLAST of Tan0000332 vs. NCBI nr
Match: 1NIO_A (Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution [Luffa aegyptiaca])

HSP 1 Score: 364.8 bits (935), Expect = 6.8e-97
Identity = 196/245 (80.00%), Postives = 215/245 (87.76%), Query Frame = 0


Query: 25  DVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPLLLPSASGINRYTLMKLSNYDGKAI 84
           +VSFSLSG  + SY KFI  LRKALPSKE V  IPLLLPSASG +RY LM+LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVFKGSTVFTLPYSGNYERLQIAAGKK 144
           TMAIDVTNVYIMGYLVN+TSYFFNESDAK ASQYVFKGST+ TLPYSGNYERLQ AAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKV 121

Query: 145 REIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQTTAEASRYKYIEQQILERAYKDEAP 204
           RE IPLGF+A DSAIT+LFHYDST+AAGA LVIIQTTAEASR+KYIE QI++R  K+E P
Sbjct: 122 REKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIKRIPKNEVP 181

Query: 205 SLATISLENSWSALSKQIQLAQTNNGNFKTSVVITDNKGRPVEIKNVSSKVVTNNIKLLL 264
           S A +SLEN WSALSKQIQLAQTNNG F+T VVI DNKG+ VEIK+V+SKVVTNNIKLLL
Sbjct: 182 SPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLLL 241

Query: 265 NKQNV 269
           NKQN+
Sbjct: 242 NKQNI 246

[Close]

BLAST of Tan0000332 vs. NCBI nr
Match: XP_022156707.1 (ribosome-inactivating protein momordin I [Momordica charantia] >P16094.2 RecName: Full=Ribosome-inactivating protein momordin I; AltName: Full=Alpha-momorcharin; Short=Alpha-MMC; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Momordica charantia] >6LOQ_A crystal structure of alpha-momorcharin in complex with cAMP [Momordica charantia] >6LOR_A crystal structure of alpha-momorcharin in complex with ADP [Momordica charantia] >6LOV_A crystal structure of alpha-momorcharin in complex with xanthosine [Momordica charantia] >6LOW_A crystal structure of alpha-momorcharin in complex with GMP [Momordica charantia] >6LOY_A crystal structure of alpha-momorcharin in complex with dAMP [Momordica charantia] >6LOZ_A crystal structure of alpha-momorcharin in complex with adenine [Momordica charantia] >6LP0_A crystal structure of alpha-momorcharin in complex with AMP [Momordica charantia] >CAA40869.1 alpha-momorcharin [Momordica charantia])

HSP 1 Score: 354.8 bits (909), Expect = 7.0e-94
Identity = 199/282 (70.57%), Postives = 225/282 (79.79%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEGDVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPL 60
           M RF VLS LIL IFL   S V+GDVSF LSG    SY  FI+DLR ALP +E VY IPL
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVF 120
           LLPS SG  RY LM L NYDGK IT+A+DVTNVYIMGYL +TTSYFFNE  A+ ASQYVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KGS-TVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQ 180
           + +    TLPYSGNYERLQIAAGK RE IP+G  ALDSAI+TL HYDST+AAGALLV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 TTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVIT 240
           TTAEA+R+KYIEQQI ERAY+DE PSLATISLENSWS LSKQIQLAQ NNG F+T +V+ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNKGRPVEIKNVSSKVVTNNIKLLLNKQNVV-GFDDYVSTKH 280
           DNKG  V+I NV+SKVVT+NI+LLLN +N+  G +  VST H
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTTH 281

[Close]

BLAST of Tan0000332 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 7.3e-105
Identity = 217/281 (77.22%), Postives = 237/281 (84.34%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEG-DVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIP 60
           M RF  LSLLI+ I   A  TVEG +VSFSLSG  + SY KFI  LRKALPSKE V  IP
Sbjct: 1   MNRFTFLSLLIILI---AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIP 60

Query: 61  LLLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYV 120
           LLLPSASG +RY LM+LSNYD KAITMAIDVTNVYIMGYLVN+TSYFFNESDAK ASQYV
Sbjct: 61  LLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYV 120

Query: 121 FKGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQ 180
           FKGST+ TLPYSGNYERLQ AAGK RE IPLGF+A DSAIT+LFHYDST+AAGA LVIIQ
Sbjct: 121 FKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQ 180

Query: 181 TTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVIT 240
           TTAEASR+KYIE QI+ER  K+E PS A +SLEN WSALSKQIQLAQTNNG F+T VVI 
Sbjct: 181 TTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVII 240

Query: 241 DNKGRPVEIKNVSSKVVTNNIKLLLNKQNVVGFDDYVSTKH 280
           DNKG+ VEIK+V+SKVVTNNIKLLLNKQN+  FDD + TKH
Sbjct: 241 DNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 278

[Close]

BLAST of Tan0000332 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 7.3e-105
Identity = 217/281 (77.22%), Postives = 237/281 (84.34%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEG-DVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIP 60
           M RF  LSLLI+ I   A  TVEG +VSFSLSG  + SY KFI  LRKALPSKE V  IP
Sbjct: 1   MNRFTFLSLLIILI---AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIP 60

Query: 61  LLLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYV 120
           LLLPSASG +RY LM+LSNYD KAITMAIDVTNVYIMGYLVN+TSYFFNESDAK ASQYV
Sbjct: 61  LLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYV 120

Query: 121 FKGSTVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQ 180
           FKGST+ TLPYSGNYERLQ AAGK RE IPLGF+A DSAIT+LFHYDST+AAGA LVIIQ
Sbjct: 121 FKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQ 180

Query: 181 TTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVIT 240
           TTAEASR+KYIE QI+ER  K+E PS A +SLEN WSALSKQIQLAQTNNG F+T VVI 
Sbjct: 181 TTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVII 240

Query: 241 DNKGRPVEIKNVSSKVVTNNIKLLLNKQNVVGFDDYVSTKH 280
           DNKG+ VEIK+V+SKVVTNNIKLLLNKQN+  FDD + TKH
Sbjct: 241 DNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAFDDGIPTKH 278

[Close]

BLAST of Tan0000332 vs. ExPASy TrEMBL
Match: A0A6J1DSN8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 3.4e-94
Identity = 199/282 (70.57%), Postives = 225/282 (79.79%), Query Frame = 0


Query: 1   MKRFLVLSLLILTIFLEAVSTVEGDVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPL 60
           M RF VLS LIL IFL   S V+GDVSF LSG    SY  FI+DLR ALP +E VY IPL
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLPSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVF 120
           LLPS SG  RY LM L NYDGK IT+A+DVTNVYIMGYL +TTSYFFNE  A+ ASQYVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KGS-TVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQ 180
           + +    TLPYSGNYERLQIAAGK RE IP+G  ALDSAI+TL HYDST+AAGALLV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 TTAEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVIT 240
           TTAEA+R+KYIEQQI ERAY+DE PSLATISLENSWS LSKQIQLAQ NNG F+T +V+ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNKGRPVEIKNVSSKVVTNNIKLLLNKQNVV-GFDDYVSTKH 280
           DNKG  V+I NV+SKVVT+NI+LLLN +N+  G +  VST H
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTTH 281

[Close]

BLAST of Tan0000332 vs. ExPASy TrEMBL
Match: S6CNX3 (rRNA N-glycosidase (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.3e-93
Identity = 198/280 (70.71%), Postives = 224/280 (80.00%), Query Frame = 0


Query: 3   RFLVLSLLILTIFLEAVSTVEGDVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPLLL 62
           RF VLS LIL IFL   S V+GDVSF LSG    SY  FI+DLR ALP +E VY IPLLL
Sbjct: 1   RFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLL 60

Query: 63  PSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVFKG 122
           PS SG  RY LM L NYDGK IT+A+DVTNVYIMGYL +TTSYFFNE  A+ ASQYVF+ 
Sbjct: 61  PSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRD 120

Query: 123 S-TVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQTT 182
           +    TLPYSGNYERLQIAAGK RE IP+G  ALDSAI+TL HYDST+AAGALLV+IQTT
Sbjct: 121 ARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTT 180

Query: 183 AEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVITDN 242
           AEA+R+KYIEQQI ERAY+DE PSLATISLENSWS LSKQIQLAQ NNG F+T +V+ DN
Sbjct: 181 AEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDN 240

Query: 243 KGRPVEIKNVSSKVVTNNIKLLLNKQNVV-GFDDYVSTKH 280
           KG  V+I NV+SKVVT+NI+LLLN +N+  G +  VST H
Sbjct: 241 KGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTTH 279

[Close]

BLAST of Tan0000332 vs. ExPASy TrEMBL
Match: S6CN87 (rRNA N-glycosidase (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.8e-93
Identity = 197/280 (70.36%), Postives = 223/280 (79.64%), Query Frame = 0


Query: 3   RFLVLSLLILTIFLEAVSTVEGDVSFSLSGG-TASYEKFIEDLRKALPSKELVYKIPLLL 62
           RF VLS LIL IFL   S V+GDVSF LSG    SY  FI+DLR ALP +E VY IPLLL
Sbjct: 1   RFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLL 60

Query: 63  PSASGINRYTLMKLSNYDGKAITMAIDVTNVYIMGYLVNTTSYFFNESDAKSASQYVFKG 122
           PS SG  RY LM L NYDGK IT+A+DVTNVYIMGYL +TTSYFFNE  A+ ASQYVF+ 
Sbjct: 61  PSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRD 120

Query: 123 S-TVFTLPYSGNYERLQIAAGKKREIIPLGFQALDSAITTLFHYDSTSAAGALLVIIQTT 182
           +    TLPYSGNYERLQIAAGK RE IP+G  ALDSAI+TL HYDST+ AGALLV+IQTT
Sbjct: 121 ARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAVAGALLVLIQTT 180

Query: 183 AEASRYKYIEQQILERAYKDEAPSLATISLENSWSALSKQIQLAQTNNGNFKTSVVITDN 242
           AEA+R+KYIEQQI ERAY+DE PSLATISLENSWS LSKQIQLAQ NNG F+T +V+ DN
Sbjct: 181 AEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDN 240

Query: 243 KGRPVEIKNVSSKVVTNNIKLLLNKQNVV-GFDDYVSTKH 280
           KG  V+I NV+SKVVT+NI+LLLN +N+  G +  VST H
Sbjct: 241 KGNRVQITNVTSKVVTSNIQLLLNTRNIAEGDNGDVSTTH 279

The following BLAST results are available for this feature:

BLAST of Tan0000332 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00465	5.7e-107	77.58	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	9.2e-97	70.57	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
P84530	2.5e-94	78.84	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P22851	3.4e-91	75.00	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q9FRX4	6.8e-84	60.79	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]

BLAST of Tan0000332 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513713.1	8.2e-119	82.86	ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo]	[more]
ABY71834.1	1.5e-104	77.22	ribosome-inactivating protein [Luffa acutangula] >CAA44230.1 b-luffin [Luffa aeg...	[more]
Q00465.1	4.4e-104	77.58	RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g...	[more]
1NIO_A	6.8e-97	80.00	Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolu...	[more]
XP_022156707.1	7.0e-94	70.57	ribosome-inactivating protein momordin I [Momordica charantia] >P16094.2 RecName...	[more]

BLAST of Tan0000332 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B0EVM6	7.3e-105	77.22	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	7.3e-105	77.22	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1DSN8	3.4e-94	70.57	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1	[more]
S6CNX3	1.3e-93	70.71	rRNA N-glycosidase (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=3 SV=1	[more]
S6CN87	3.8e-93	70.36	rRNA N-glycosidase (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=3 SV=1	[more]

BLAST of Tan0000332 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 35..48 score: 43.42 coord: 173..193 score: 59.27 coord: 82..97 score: 50.99 coord: 205..222 score: 53.22 coord: 144..158 score: 55.61
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 185..269 e-value: 1.7E-27 score: 97.1
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 35..222 e-value: 5.9E-31 score: 107.7
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 33..266
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..184 e-value: 2.2E-43 score: 149.7
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 33..266
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 177..193
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 26..266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0000332.1	Tan0000332.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity