Homology
BLAST of Tan0000139 vs. ExPASy Swiss-Prot
Match:
P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)
HSP 1 Score: 1062.4 bits (2746), Expect = 1.9e-309
Identity = 497/581 (85.54%), Postives = 529/581 (91.05%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FL++FF L++ +FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLILFFFGLIL-AFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVEL NKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH ++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 MPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTRGRHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVKSNRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMK+ L AF
Sbjct: 364 RITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ TK GNGVYQF++GEIVDVILQNANMMKENLSEIHPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKF+A +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACGGTA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Tan0000139 vs. ExPASy Swiss-Prot
Match:
P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)
HSP 1 Score: 1040.8 bits (2690), Expect = 5.8e-303
Identity = 478/549 (87.07%), Postives = 509/549 (92.71%), Query Frame = 0
Query: 33 AQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANAGDTVVVELTNKLHTEGLVIH 92
+QIRHYKWEVEYMFW+P+C ENIVMGI+GQFPGPTIRANAGD+VVVELTNKLHTEG+VIH
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 93 WHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLI 152
WHGILQRGTPWADGTASISQCAINPGETF Y F VD PGTFFYHGHLGMQRSAGLYGSLI
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 153 VDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNPIRWIGEPQSILLNGRGQFDC 212
VDPPQG KEPFHYD EINLLLSDWWH+SIHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDC
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180
Query: 213 SIAAKYNSELKQCALKGGEQCAPFIFHVHPKKTYRIRIASTTALAALNIAIANHRMIVVE 272
SIAAKY+S L+ C LKG E CAP+IFHV PKKTYRIRIASTTALAALN AI NH+++VVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240
Query: 273 ADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWISVGTRGRHPNTPPGLTLLNYLP 332
ADGNYVQ F DIDIYSGESYSVLITTDQNPSENYW+SVGTR RHPNTPPGLTLLNYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300
Query: 333 NSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKPPVKSNRRIFLLNTQNQINGVA 392
NSVSKLP SPPP+TPAWDDFDRSKNFTYRITAAMGSPKPPVK NRRIFLLNTQN ING
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYV 360
Query: 393 KWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQ 452
KWAIN+VSLALPPTPYLGAMKY L AFDQ PPPE FP +YDID+PP+N+ T+ GNGVYQ
Sbjct: 361 KWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQ 420
Query: 453 FQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFSATDENTFNLKNPPLR 512
F++GE+VDVILQNANMMKENLSE HPWHLHGHDFWVLGYGDGKFSA +E++ NLKNPPLR
Sbjct: 421 FKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLR 480
Query: 513 NTVVIFPFGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEAVHKVGRIPTKALACGG 572
NTVVIFP+GWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAE V KVGRIPTKALACGG
Sbjct: 481 NTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGG 540
Query: 573 TANTLLNNP 582
TA +L+NNP
Sbjct: 541 TAKSLINNP 549
BLAST of Tan0000139 vs. ExPASy Swiss-Prot
Match:
P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)
HSP 1 Score: 993.4 bits (2567), Expect = 1.1e-288
Identity = 465/573 (81.15%), Postives = 502/573 (87.61%), Query Frame = 0
Query: 6 EPLLTSPFLVIFFVLLMISSFG-TFSEAA--QIRHYKWEVEYMFWSPDCQENIVMGIDGQ 65
+P PFL F VL +I FG T SEA +I+HYKW+VEYMFWSPDC ENIVMGI+G+
Sbjct: 7 KPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGE 66
Query: 66 FPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFT 125
FPGPTIRANAGD VVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGETFT
Sbjct: 67 FPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFT 126
Query: 126 YRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIH 185
YRFVVDK GT+FYHGHLGMQRSAGLYGSLIVDPP+G EPFHYDEEINLLLSDWWH+S+H
Sbjct: 127 YRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVH 186
Query: 186 KQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHVHP 245
KQEVGLSS P+RWIGEPQSIL+NG+GQFDCSIAAKYN LKQC L G E+CAPFI HV P
Sbjct: 187 KQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQP 246
Query: 246 KKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQ 305
KKTYRIRIASTTALA+LN AI NH ++VVEADGNYVQ F DIDIYSGESYSVLITTDQ
Sbjct: 247 KKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQ 306
Query: 306 NPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRI 365
NP ENYW+S+G R R P TPPGLTLLNYLPNS SKLPISPPPETP W+DFDRSKNFT+RI
Sbjct: 307 NPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRI 366
Query: 366 TAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQ 425
AAMGSPKPPV+ NRR+FLLNTQN+ING KWAINNVSLALPPTPYL AMK +L +AF+Q
Sbjct: 367 FAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQ 426
Query: 426 TPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLH 485
PPPE FP+ YDI++PP N T TGNGVY+F MGE VDVILQNANM+ N+SEIHPWHLH
Sbjct: 427 NPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLH 486
Query: 486 GHDFWVLGYGDGKFSA-TDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHI 545
GHDFWVLGYG+GKF A DE NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCHI
Sbjct: 487 GHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 546
Query: 546 EPHLHMGMGVVFAEAVHKVGRIPTKALACGGTA 575
EPHLHMGMGVVFAE VH VG IP KALACG TA
Sbjct: 547 EPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA 578
BLAST of Tan0000139 vs. ExPASy Swiss-Prot
Match:
Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)
HSP 1 Score: 836.6 bits (2160), Expect = 1.7e-241
Identity = 384/569 (67.49%), Postives = 460/569 (80.84%), Query Frame = 0
Query: 13 FLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANA 72
F ++ +LL +SS + AA+ RH+KW+VEY+ WSPD +E++VMGI+GQFPGPTIRA A
Sbjct: 9 FFLLPLILLELSSSRSVM-AAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKA 68
Query: 73 GDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGT 132
GDTV V LTNKLHTEG+VIHWHGI Q GTPWADGTA+ISQCAINPGETF YRF VDK GT
Sbjct: 69 GDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGT 128
Query: 133 FFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNP 192
+FYHGH GMQRSAGLYGSLIV+ +G KEPFHYD E NLLLSDWWH+ H+QEV LSSNP
Sbjct: 129 YFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSNP 188
Query: 193 IRWIGEPQSILLNGRGQFDCSIAAKYNS-ELKQCALKGGEQCAPFIFHVHPKKTYRIRIA 252
+RWIGEPQ++LLNGRGQ++CS+AA+++ L QC L+GGEQ AP I V P K YR+R+A
Sbjct: 189 LRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLRVA 248
Query: 253 STTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWIS 312
STTAL +L++AI H+M+VVEADGNYVQ F V D+DIYSGESYSVL TDQ+P++NYWIS
Sbjct: 249 STTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWIS 308
Query: 313 VGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKP 372
+ RGR P TP GLTLLNYLPNS SK P PPP P W+D++ SK+F+ +I A MGSPKP
Sbjct: 309 INVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKP 368
Query: 373 PVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPV 432
P +++RRI LLNTQN+I+G KWAINNVSL LP YLG+++Y + +AFD PPP+NFP
Sbjct: 369 PPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDNFPK 428
Query: 433 EYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGY 492
+YD+ N N+ GNGVY + +D+ILQNAN + +++SEIHPWHLHGHDFWVLGY
Sbjct: 429 DYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGY 488
Query: 493 GDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHIEPHLHMGMGV 552
G+GKFS D FNLKNPPLRNT VIFPFGWTA+RFV DNPGVWAFHCHIEPHLHMGMGV
Sbjct: 489 GEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGV 548
Query: 553 VFAEAVHKVGRIPTKALACGGTANTLLNN 581
+FAE VH V +IP +ALACG T L++N
Sbjct: 549 IFAEGVHLVKKIPKEALACGLTGKMLMSN 575
BLAST of Tan0000139 vs. ExPASy Swiss-Prot
Match:
M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)
HSP 1 Score: 813.1 bits (2099), Expect = 2.0e-234
Identity = 367/550 (66.73%), Postives = 440/550 (80.00%), Query Frame = 0
Query: 32 AAQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANAGDTVVVELTNKLHTEGLVI 91
+A +R Y WEVEY F PDC+E +VM ++GQFPGPTI A AGDT+VV LTNKL TEGLVI
Sbjct: 17 SAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLVI 76
Query: 92 HWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGTFFYHGHLGMQRSAGLYGSL 151
HWHGI Q G+PWADG A ++QCAI+PGETFTY F VDKPGT FYHGH GMQRSAGLYGSL
Sbjct: 77 HWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYGSL 136
Query: 152 IVDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNPIRWIGEPQSILLNGRGQFD 211
I+D +G KEP YD E NLLLSDWWHE + QE+GLSS P+RWIGE QSIL+NGRGQF+
Sbjct: 137 IIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILINGRGQFN 196
Query: 212 CSIAAKYNS-ELKQCALKGGEQCAPFIFHVHPKKTYRIRIASTTALAALNIAIANHRMIV 271
CS+AA+++S L C K G+QCAP HV P KTYRIR+AS+TALA+LN A+ H+++V
Sbjct: 197 CSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGHKLVV 256
Query: 272 VEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWISVGTRGRHPNTPPGLTLLNY 331
VEADGNY+ F DDIDIYSGE+YSVL+TTDQ+PS+NY+I+ G RGR PNTPP LT+LNY
Sbjct: 257 VEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALTVLNY 316
Query: 332 LPNSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKPPVKSNRRIFLLNTQNQING 391
+ S+LP SPPPETP W+DFDRSKNF+ +I AAMGSP PP + R+ LLNTQN I G
Sbjct: 317 VTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERLILLNTQNLIEG 376
Query: 392 VAKWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPVEYDIDSPPSNKNTKTGNGV 451
KWAINNVSLA+P TPYLG++KY L + F+++ PP+++PV+YDI +PP N+N K GN
Sbjct: 377 FTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPPRNRNAKQGNVS 436
Query: 452 YQFQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFS-ATDENTFNLKNP 511
F VDVILQNAN + N SEIHPWHLHGHDFWVLGYG+GKF DE T+NLKNP
Sbjct: 437 CVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKTYNLKNP 496
Query: 512 PLRNTVVIFPFGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEAVHKVGRIPTKALA 571
PLRNTV ++P+GWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAE ++++G++P +AL
Sbjct: 497 PLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALG 556
Query: 572 CGGTANTLLN 580
CG T L+N
Sbjct: 557 CGLTKQFLMN 566
BLAST of Tan0000139 vs. NCBI nr
Match:
XP_022967157.1 (L-ascorbate oxidase [Cucurbita maxima] >P24792.2 RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags: Precursor [Cucurbita maxima] >BAA09528.1 ascorbate oxidase [Cucurbita maxima])
HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 497/581 (85.54%), Postives = 529/581 (91.05%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FL++FF L++ +FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLILFFFGLIL-AFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVEL NKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH ++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 MPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTRGRHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVKSNRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMK+ L AF
Sbjct: 364 RITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ TK GNGVYQF++GEIVDVILQNANMMKENLSEIHPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKF+A +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACGGTA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Tan0000139 vs. NCBI nr
Match:
XP_023531516.1 (L-ascorbate oxidase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1059.7 bits (2739), Expect = 9.2e-306
Identity = 495/581 (85.20%), Postives = 527/581 (90.71%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FLV+FF L++ FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLVLFFFGLIL-GFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGD+VVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSESCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH+++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 SPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTR RHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVK NRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMKY L AF
Sbjct: 364 RITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ T+ GNGVYQF++GE+VDVILQNANMMKENLSE HPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKFSA +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACGGTA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Tan0000139 vs. NCBI nr
Match:
CAA39300.1 (ascorbate oxidase [Cucurbita hybrid cultivar])
HSP 1 Score: 1057.4 bits (2733), Expect = 4.6e-305
Identity = 496/581 (85.37%), Postives = 528/581 (90.88%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FL++FF L++ +FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLILFFFGLIL-AFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVEL NKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDW H+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWCHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH ++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 MPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTRGRHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVKSNRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMK+ L AF
Sbjct: 364 RITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ TK GNGVYQF++GEIVDVILQNANMMKENLSEIHPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKF+A +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACGGTA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Tan0000139 vs. NCBI nr
Match:
XP_022929646.1 (L-ascorbate oxidase [Cucurbita moschata])
HSP 1 Score: 1057.0 bits (2732), Expect = 6.0e-305
Identity = 495/581 (85.20%), Postives = 526/581 (90.53%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FLV+FF L++ FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLVLFFFGLIL-GFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH+++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 SPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTR RHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVK NRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMKY L AF
Sbjct: 364 RITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ T+ GNGVYQF++GE+VDVILQNANMMKENLSE HPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKFSA +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACG TA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVDKVGRIPTKALACGVTAKSLINNP 579
BLAST of Tan0000139 vs. NCBI nr
Match:
KAG6588158.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022051.1 L-ascorbate oxidase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1053.9 bits (2724), Expect = 5.0e-304
Identity = 493/581 (84.85%), Postives = 526/581 (90.53%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FLV+FF L++ FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLVLFFFGLIL-GFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH+++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 SPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTR RHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVK NRRIFLLNTQN I+G KWAIN+VSLALPPTPYLGAMKY L AF
Sbjct: 364 RITAAMGSPKPPVKFNRRIFLLNTQNVISGYVKWAINDVSLALPPTPYLGAMKYNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ T+ GNGVYQF++GE+VDVILQNANMMKENLSE HPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKFSA +E++ NLKNPPL+NTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFSAEEESSLNLKNPPLKNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACG TA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVDKVGRIPTKALACGVTAKSLINNP 579
BLAST of Tan0000139 vs. ExPASy TrEMBL
Match:
A0A6J1HPZ9 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466657 PE=3 SV=1)
HSP 1 Score: 1062.4 bits (2746), Expect = 6.9e-307
Identity = 497/581 (85.54%), Postives = 529/581 (91.05%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FL++FF L++ +FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLILFFFGLIL-AFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVEL NKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH ++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 MPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTRGRHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVKSNRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMK+ L AF
Sbjct: 364 RITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ TK GNGVYQF++GEIVDVILQNANMMKENLSEIHPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKF+A +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACGGTA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Tan0000139 vs. ExPASy TrEMBL
Match:
A0A6J1ENQ7 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1)
HSP 1 Score: 1057.0 bits (2732), Expect = 2.9e-305
Identity = 495/581 (85.20%), Postives = 526/581 (90.53%), Query Frame = 0
Query: 1 MGKLGEPLLTSPFLVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGID 60
MGK EP FLV+FF L++ FG SE +QIRHYKWEVEYMFW+PDC ENIVMGI+
Sbjct: 4 MGKAREP----NFLVLFFFGLIL-GFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGIN 63
Query: 61 GQFPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGET 120
GQFPGPTIRANAGDTVVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGET
Sbjct: 64 GQFPGPTIRANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGET 123
Query: 121 FTYRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHES 180
F Y F VD PGTFFYHGHLGMQRSAGLYGSLIVDPPQG KEPFHYD EINLLLSDWWH+S
Sbjct: 124 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 183
Query: 181 IHKQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHV 240
IHKQEVGLSS PIRWIGEPQ+ILLNGRGQFDCSIAAKY+S L+ C LKG E CAP+IFHV
Sbjct: 184 IHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHV 243
Query: 241 HPKKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITT 300
PKKTYRIRIASTTALAALN AI NH+++VVEADGNYVQ F DIDIYSGESYSVLITT
Sbjct: 244 SPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITT 303
Query: 301 DQNPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTY 360
DQNPSENYW+SVGTR RHPNTPPGLTLLNYLPNSVSKLP SPPPETPAWDDFDRSKNFTY
Sbjct: 304 DQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTY 363
Query: 361 RITAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAF 420
RITAAMGSPKPPVK NRRIFLLNTQN ING KWAIN+VSLALPPTPYLGAMKY L AF
Sbjct: 364 RITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAF 423
Query: 421 DQTPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWH 480
DQ PPPE FP +YDID+PP+N+ T+ GNGVYQF++GE+VDVILQNANMMKENLSE HPWH
Sbjct: 424 DQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWH 483
Query: 481 LHGHDFWVLGYGDGKFSATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCH 540
LHGHDFWVLGYGDGKFSA +E++ NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCH
Sbjct: 484 LHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCH 543
Query: 541 IEPHLHMGMGVVFAEAVHKVGRIPTKALACGGTANTLLNNP 582
IEPHLHMGMGVVFAE V KVGRIPTKALACG TA +L+NNP
Sbjct: 544 IEPHLHMGMGVVFAEGVDKVGRIPTKALACGVTAKSLINNP 579
BLAST of Tan0000139 vs. ExPASy TrEMBL
Match:
A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)
HSP 1 Score: 1009.2 bits (2608), Expect = 6.9e-291
Identity = 470/573 (82.02%), Postives = 508/573 (88.66%), Query Frame = 0
Query: 6 EPLLTSPFLVIFFVLLMISSFG-TFSEAA--QIRHYKWEVEYMFWSPDCQENIVMGIDGQ 65
+P PFLV FFVL +I FG T SEA +IRHYKWEVEYMFWSPDC ENIVMGI+GQ
Sbjct: 7 KPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQ 66
Query: 66 FPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFT 125
FPGPTIRANAGD VVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGETFT
Sbjct: 67 FPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFT 126
Query: 126 YRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIH 185
YRFVVDK GT+FYHGHLGMQRSAGLYGSLIVDP +G EPFHYDEEINLLLSDWWH+S+H
Sbjct: 127 YRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVH 186
Query: 186 KQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHVHP 245
KQEVGLSS P+RWIGEPQSIL+NGRGQFDCSIAAKY + LKQC L G EQCAPFI HVHP
Sbjct: 187 KQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHP 246
Query: 246 KKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQ 305
KKTYRIRIASTTALA+LN AI NH+++VVEADGNYVQ F DIDIYSGESYSVLITTDQ
Sbjct: 247 KKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQ 306
Query: 306 NPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRI 365
NPSENYW+S+G R R P TPPGLT+LNYLPNSVSKLPISPPPETP W+DFDRSKNFT+RI
Sbjct: 307 NPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRI 366
Query: 366 TAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQ 425
AAMGSPKPPV+ NRR+FLLNTQN+ING KWAINNVSLALPPTPYL AMK++L +AF+Q
Sbjct: 367 FAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQ 426
Query: 426 TPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLH 485
PPPE FP++YDI++PP N T TGNGVY+F MGE VDVILQNANM+ N SEIHPWHLH
Sbjct: 427 NPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLH 486
Query: 486 GHDFWVLGYGDGKFSAT-DENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHI 545
GHDFWVLGYG+GKFSA D NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCHI
Sbjct: 487 GHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 546
Query: 546 EPHLHMGMGVVFAEAVHKVGRIPTKALACGGTA 575
EPHLHMGMGV+FAE VHKVG IP A+ACG TA
Sbjct: 547 EPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA 578
BLAST of Tan0000139 vs. ExPASy TrEMBL
Match:
Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)
HSP 1 Score: 1006.9 bits (2602), Expect = 3.4e-290
Identity = 469/573 (81.85%), Postives = 507/573 (88.48%), Query Frame = 0
Query: 6 EPLLTSPFLVIFFVLLMISSFG-TFSEAA--QIRHYKWEVEYMFWSPDCQENIVMGIDGQ 65
+P PFLV FFVL +I FG T SEA +IRHYKWEVEYMFWSPDC ENIVMGI+GQ
Sbjct: 7 KPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQ 66
Query: 66 FPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFT 125
FPGPTIRANAGD VVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGETFT
Sbjct: 67 FPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFT 126
Query: 126 YRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIH 185
YRFVVDK GT+FYHGHLGMQRSAGLYGSLIVDP +G EPFHYDEEINLLLSDWWH+S+H
Sbjct: 127 YRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVH 186
Query: 186 KQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHVHP 245
KQEVGLSS P+RWIGEPQSIL+NGRGQFDCSIAAKY + LKQC L G EQCAPFI HVHP
Sbjct: 187 KQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHP 246
Query: 246 KKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQ 305
KK YRIRIASTTALA+LN AI NH+++VVEADGNYVQ F DIDIYSGESYSVLITTDQ
Sbjct: 247 KKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQ 306
Query: 306 NPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRI 365
NPSENYW+S+G R R P TPPGLT+LNYLPNSVSKLPISPPPETP W+DFDRSKNFT+RI
Sbjct: 307 NPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSKNFTFRI 366
Query: 366 TAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQ 425
AAMGSPKPPV+ NRR+FLLNTQN+ING KWAINNVSLALPPTPYL AMK++L +AF+Q
Sbjct: 367 FAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKFRLSTAFNQ 426
Query: 426 TPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLH 485
PPPE FP++YDI++PP N T TGNGVY+F MGE VDVILQNANM+ N SEIHPWHLH
Sbjct: 427 NPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSEIHPWHLH 486
Query: 486 GHDFWVLGYGDGKFSAT-DENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHI 545
GHDFWVLGYG+GKFSA D NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCHI
Sbjct: 487 GHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 546
Query: 546 EPHLHMGMGVVFAEAVHKVGRIPTKALACGGTA 575
EPHLHMGMGV+FAE VHKVG IP A+ACG TA
Sbjct: 547 EPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA 578
BLAST of Tan0000139 vs. ExPASy TrEMBL
Match:
E7BBM8 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)
HSP 1 Score: 993.8 bits (2568), Expect = 3.0e-286
Identity = 465/573 (81.15%), Postives = 503/573 (87.78%), Query Frame = 0
Query: 6 EPLLTSPFLVIFFVLLMISSFG-TFSEAA--QIRHYKWEVEYMFWSPDCQENIVMGIDGQ 65
+P PFL F VL +I FG T SEA +I+HYKW+VEYMFWSPDC ENIVMGI+G+
Sbjct: 7 KPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGE 66
Query: 66 FPGPTIRANAGDTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFT 125
FPGPTIRANAGD VVVELTNKLHTEG+VIHWHGILQRGTPWADGTASISQCAINPGETFT
Sbjct: 67 FPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFT 126
Query: 126 YRFVVDKPGTFFYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIH 185
YRFVVDK GT+FYHGHLGMQRSAGLYGSLIVDPP+G EPFHYDEEINLLLSDWWH+S+H
Sbjct: 127 YRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVH 186
Query: 186 KQEVGLSSNPIRWIGEPQSILLNGRGQFDCSIAAKYNSELKQCALKGGEQCAPFIFHVHP 245
KQEVGLSS P+RWIGEPQSIL+NG+GQFDCSIAAKYN LKQC L G E+CAPFI HV P
Sbjct: 187 KQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQP 246
Query: 246 KKTYRIRIASTTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQ 305
KKTYRIRIASTTALA+LN AI NH ++VVEADGNYVQ F DIDIYSGESYSVLITTDQ
Sbjct: 247 KKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQ 306
Query: 306 NPSENYWISVGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRI 365
NP ENYW+S+G R R P TPPGLTLLNYLPNS SKLPISPPPETP W+DFDRSKNFT+RI
Sbjct: 307 NPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRI 366
Query: 366 TAAMGSPKPPVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQ 425
AAMGSPKPPV+ NRR+FLLNTQN+ING KWAINNVSLALPPTPYL AMK +L +AF+Q
Sbjct: 367 FAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQ 426
Query: 426 TPPPENFPVEYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLH 485
+PPPE FP+ YDI++PP N T TGNGVY+F MGE VDVILQNANM+ N+SEIHPWHLH
Sbjct: 427 SPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLH 486
Query: 486 GHDFWVLGYGDGKFSA-TDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHI 545
GHDFWVLGYG+GKF A DE NLKNPPLRNTVVIFP+GWTAIRFVADNPGVWAFHCHI
Sbjct: 487 GHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 546
Query: 546 EPHLHMGMGVVFAEAVHKVGRIPTKALACGGTA 575
EPHLHMGMGVVFAE VH VG IP KALACG TA
Sbjct: 547 EPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA 578
BLAST of Tan0000139 vs. TAIR 10
Match:
AT5G21105.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 817.4 bits (2110), Expect = 7.4e-237
Identity = 370/567 (65.26%), Postives = 453/567 (79.89%), Query Frame = 0
Query: 16 IFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANAGDT 75
+++++L+++ T + +A +R Y WEVEY +WSPDC+E VM ++G+FPGPTI+A AGDT
Sbjct: 19 VWWIVLVVAVL-THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDT 78
Query: 76 VVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGTFFY 135
+VV LTNKL TEGLVIHWHGI Q G+PWADG A ++QCAINPGETFTY F V+KPGT FY
Sbjct: 79 IVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFY 138
Query: 136 HGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNPIRW 195
HGH GMQRSAGLYGSLIVD +G E YD E NLLLSDWWHE+I QE+GLSS P+RW
Sbjct: 139 HGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRW 198
Query: 196 IGEPQSILLNGRGQFDCSIAAKY--NSELKQCALKGGEQCAPFIFHVHPKKTYRIRIAST 255
IGE QSIL+NGRGQF+CS+AA++ N+ L C K G+QCAP I HV P KTYRIR++ST
Sbjct: 199 IGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSST 258
Query: 256 TALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWISVG 315
TALA+LN+A+ H+++VVEADGNY+ F DDIDIYSGESYSVL+TTDQ+PS+NY+ISVG
Sbjct: 259 TALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVG 318
Query: 316 TRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKPPV 375
RGR PNT LT+LNY+ SKLP SPPP TP WDDF+RSKNF+ +I +AMGSP PP
Sbjct: 319 VRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPK 378
Query: 376 KSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPVEY 435
K +R+ LLNTQN I+G KWAINNVSL P TPYLG++KY L+ F++ PP ++ ++Y
Sbjct: 379 KYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDY 438
Query: 436 DIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGD 495
DI +PP NT TGNG+Y F VDVI+QNAN++K +SEIHPWHLHGHDFWVLGYGD
Sbjct: 439 DIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGD 498
Query: 496 GKFS-ATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVV 555
GKF DE T+NLKNPPLRNT +++P+GWTAIRFV DNPGVW FHCHIEPHLHMGMGVV
Sbjct: 499 GKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVV 558
Query: 556 FAEAVHKVGRIPTKALACGGTANTLLN 580
FAE ++++G++P +AL CG T L+N
Sbjct: 559 FAEGLNRIGKVPDEALGCGLTKQFLMN 584
BLAST of Tan0000139 vs. TAIR 10
Match:
AT5G21100.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 751.1 bits (1938), Expect = 6.5e-217
Identity = 354/571 (62.00%), Postives = 431/571 (75.48%), Query Frame = 0
Query: 15 VIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANAGD 74
VI + LL + S +A + WEVEY +W PDC+E IVM I+GQFPGPTI A AGD
Sbjct: 3 VIVWWLLTVVVVAFHSASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGD 62
Query: 75 TVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGTFF 134
TV++ + NKL TEG+VIHWHGI Q+GTPWADG A ++QC INPGETFTY+F+VDK GT F
Sbjct: 63 TVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHF 122
Query: 135 YHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNPIR 194
YHGH GMQRS+GLYG LIV P KE YD E NLLLSDWWH+SIH QE+ LSS P+R
Sbjct: 123 YHGHYGMQRSSGLYGMLIVRSP---KERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMR 182
Query: 195 WIGEPQSILLNGRGQFDCSIAAKYN--SELKQCALKGGEQCAPFIFHVHPKKTYRIRIAS 254
WIGEPQS+L+NGRGQF+CS AA +N E C K +QCAP V P + YR+RIAS
Sbjct: 183 WIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIAS 242
Query: 255 TTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWISV 314
TTALA+LN+A+ H+++VVEADGNYV F V+DID+YSGE+YSVL+ T+ PS+ YWISV
Sbjct: 243 TTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISV 302
Query: 315 GTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKPP 374
G RGR P TP LT++NY+ + S+ P PPP TP W+D DRSK+F+ +I AA G PKPP
Sbjct: 303 GVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPP 362
Query: 375 VKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPVE 434
KS+ ++ LLNTQN KW+INNVSL++P TPYLG+++Y L+SA+D P + ++
Sbjct: 363 EKSHDQLILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMD 422
Query: 435 -YDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGY 494
YDI PP N NT G+G+Y F G +VDVILQNAN++K +SEIHPWH+HGHDFWVLGY
Sbjct: 423 NYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGY 482
Query: 495 GDGKFS-ATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHIEPHLHMGMG 554
G+GKF DE TFNLKNPPLRNTVV++PFGWTAIRFV DNPGVW FHCHIEPHLHMGMG
Sbjct: 483 GEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMG 542
Query: 555 VVFAEAVHKVGR--IPTKALACGGTANTLLN 580
VVF E V ++G+ IP +AL CG T L+N
Sbjct: 543 VVFVEGVDRIGKMEIPDEALGCGLTRKWLMN 569
BLAST of Tan0000139 vs. TAIR 10
Match:
AT5G21105.3 (Plant L-ascorbate oxidase )
HSP 1 Score: 747.3 bits (1928), Expect = 9.4e-216
Identity = 340/521 (65.26%), Postives = 415/521 (79.65%), Query Frame = 0
Query: 16 IFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANAGDT 75
+++++L+++ T + +A +R Y WEVEY +WSPDC+E VM ++G+FPGPTI+A AGDT
Sbjct: 19 VWWIVLVVAVL-THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDT 78
Query: 76 VVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGTFFY 135
+VV LTNKL TEGLVIHWHGI Q G+PWADG A ++QCAINPGETFTY F V+KPGT FY
Sbjct: 79 IVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFY 138
Query: 136 HGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNPIRW 195
HGH GMQRSAGLYGSLIVD +G E YD E NLLLSDWWHE+I QE+GLSS P+RW
Sbjct: 139 HGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRW 198
Query: 196 IGEPQSILLNGRGQFDCSIAAKY--NSELKQCALKGGEQCAPFIFHVHPKKTYRIRIAST 255
IGE QSIL+NGRGQF+CS+AA++ N+ L C K G+QCAP I HV P KTYRIR++ST
Sbjct: 199 IGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSST 258
Query: 256 TALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWISVG 315
TALA+LN+A+ H+++VVEADGNY+ F DDIDIYSGESYSVL+TTDQ+PS+NY+ISVG
Sbjct: 259 TALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVG 318
Query: 316 TRGRHPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKPPV 375
RGR PNT LT+LNY+ SKLP SPPP TP WDDF+RSKNF+ +I +AMGSP PP
Sbjct: 319 VRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPK 378
Query: 376 KSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPVEY 435
K +R+ LLNTQN I+G KWAINNVSL P TPYLG++KY L+ F++ PP ++ ++Y
Sbjct: 379 KYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDY 438
Query: 436 DIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGD 495
DI +PP NT TGNG+Y F VDVI+QNAN++K +SEIHPWHLHGHDFWVLGYGD
Sbjct: 439 DIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGD 498
Query: 496 GKFS-ATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPG 534
GKF DE T+NLKNPPLRNT +++P+GWTAIRFV DNPG
Sbjct: 499 GKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538
BLAST of Tan0000139 vs. TAIR 10
Match:
AT4G39830.1 (Cupredoxin superfamily protein )
HSP 1 Score: 573.9 bits (1478), Expect = 1.4e-163
Identity = 282/570 (49.47%), Postives = 368/570 (64.56%), Query Frame = 0
Query: 14 LVIFFVLLMISSFGTFSEAAQIRHYKWEVEYMFWSPDCQENIVMGIDGQFPGPTIRANAG 73
+V+ F+ L SS +IR +KWEV+Y F SPDC E +V+ I+G+FPGPTI+A G
Sbjct: 17 MVLCFIALFFSSVLC---QGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQG 76
Query: 74 DTVVVELTNKLHTEGLVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKPGTF 133
DT+VVEL N TE + +HWHGI Q GTPW DG ++QC I PGE F Y+FVVD+PGT+
Sbjct: 77 DTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTY 136
Query: 134 FYHGHLGMQRSAGLYGSLIVDPPQGVKEPFHYDEEINLLLSDWWHESIHKQEVGLSSNPI 193
YH H GMQR +GL G + V PP EPF YD + N LL+DW+H+S+ ++ GL+S P
Sbjct: 137 MYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPF 196
Query: 194 RWIGEPQSILLNGRGQFDCSIAAKYNSELKQ--CALKGGEQCAPFIFHVHPKKTYRIRIA 253
+W+GEPQS+++ GRG+F+CS L C + + C+ FI V P KTYR+RI
Sbjct: 197 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNAD-CSRFILTVIPGKTYRLRIG 256
Query: 254 STTALAALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWIS 313
S TAL+AL+ I H + VVEADG+YV+ F V ++ +YSGE+YSVL+ DQNP NYWI+
Sbjct: 257 SLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWIT 316
Query: 314 VGTRGRHPNTPPGLTLLNYLPNSVSKLPISPPPET----PAWDDFDRSKNFTYRITAAMG 373
R TPP +LNY PN + P P E+ P W+D + I A G
Sbjct: 317 SSIVSRPATTPPATAVLNYYPNHPRRRP--PTSESSNIVPEWNDTRSRLAQSLAIKARRG 376
Query: 374 SPKP-PVKSNRRIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFD-QTPP 433
P S++ I LLNTQN++NG +W++NNVS P TPYL A+K L +AFD +
Sbjct: 377 FIHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTA 436
Query: 434 PENFPV-EYDIDSPPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLHGH 493
PEN+ YDI + P N N T +G+Y+ + VDVILQNAN M N SE HPWHLHGH
Sbjct: 437 PENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGH 496
Query: 494 DFWVLGYGDGKFS-ATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHIEP 553
DFWVLGYG+GKF+ + D +N +P +NTV + PFGWTA+RF ADNPGVW+FHCHIE
Sbjct: 497 DFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIES 556
Query: 554 HLHMGMGVVFAEAVHKVGRIPTKALACGGT 574
H MGMG+VF + KV +P+ + CG T
Sbjct: 557 HFFMGMGIVFESGIDKVSSLPSSIMGCGQT 580
BLAST of Tan0000139 vs. TAIR 10
Match:
AT5G21105.2 (Plant L-ascorbate oxidase )
HSP 1 Score: 543.5 bits (1399), Expect = 2.1e-154
Identity = 247/383 (64.49%), Postives = 305/383 (79.63%), Query Frame = 0
Query: 200 QSILLNGRGQFDCSIAAKY--NSELKQCALKGGEQCAPFIFHVHPKKTYRIRIASTTALA 259
+SIL+NGRGQF+CS+AA++ N+ L C K G+QCAP I HV P KTYRIR++STTALA
Sbjct: 11 KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70
Query: 260 ALNIAIANHRMIVVEADGNYVQTFEVDDIDIYSGESYSVLITTDQNPSENYWISVGTRGR 319
+LN+A+ H+++VVEADGNY+ F DDIDIYSGESYSVL+TTDQ+PS+NY+ISVG RGR
Sbjct: 71 SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130
Query: 320 HPNTPPGLTLLNYLPNSVSKLPISPPPETPAWDDFDRSKNFTYRITAAMGSPKPPVKSNR 379
PNT LT+LNY+ SKLP SPPP TP WDDF+RSKNF+ +I +AMGSP PP K +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190
Query: 380 RIFLLNTQNQINGVAKWAINNVSLALPPTPYLGAMKYKLESAFDQTPPPENFPVEYDIDS 439
R+ LLNTQN I+G KWAINNVSL P TPYLG++KY L+ F++ PP ++ ++YDI +
Sbjct: 191 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMN 250
Query: 440 PPSNKNTKTGNGVYQFQMGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFS 499
PP NT TGNG+Y F VDVI+QNAN++K +SEIHPWHLHGHDFWVLGYGDGKF
Sbjct: 251 PPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFK 310
Query: 500 -ATDENTFNLKNPPLRNTVVIFPFGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEA 559
DE T+NLKNPPLRNT +++P+GWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 311 PGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEG 370
Query: 560 VHKVGRIPTKALACGGTANTLLN 580
++++G++P +AL CG T L+N
Sbjct: 371 LNRIGKVPDEALGCGLTKQFLMN 393
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P24792 | 1.9e-309 | 85.54 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2 | [more] |
P37064 | 5.8e-303 | 87.07 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1 | [more] |
P14133 | 1.1e-288 | 81.15 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1 | [more] |
Q40588 | 1.7e-241 | 67.49 | L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1 | [more] |
M4DUF2 | 2.0e-234 | 66.73 | L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_022967157.1 | 1.4e-306 | 85.54 | L-ascorbate oxidase [Cucurbita maxima] >P24792.2 RecName: Full=L-ascorbate oxida... | [more] |
XP_023531516.1 | 9.2e-306 | 85.20 | L-ascorbate oxidase [Cucurbita pepo subsp. pepo] | [more] |
CAA39300.1 | 4.6e-305 | 85.37 | ascorbate oxidase [Cucurbita hybrid cultivar] | [more] |
XP_022929646.1 | 6.0e-305 | 85.20 | L-ascorbate oxidase [Cucurbita moschata] | [more] |
KAG6588158.1 | 5.0e-304 | 84.85 | L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022051... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1HPZ9 | 6.9e-307 | 85.54 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466657 PE=3 SV=1 | [more] |
A0A6J1ENQ7 | 2.9e-305 | 85.20 | L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1 | [more] |
A0A5A7UMY3 | 6.9e-291 | 82.02 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... | [more] |
Q9M5B4 | 3.4e-290 | 81.85 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1 | [more] |
E7BBM8 | 3.0e-286 | 81.15 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1 | [more] |