Tan0000114 (gene) Snake gourd v1

BLAST of Tan0000114 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 6.5e-232
Identity = 391/543 (72.01%), Postives = 459/543 (84.53%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           M  ETNQ  + IFRSKLPDI+IP +LPLH Y F+N ++F SRPCLI+GA  H+Y+Y DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTSR+VA+GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFD 180
           AKA+NAKLI+T ACF ++VKD A +N + ++C+D   EGC+HFS L+ ADE   P V   
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIY 240
           SDDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H  DV++CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDV 300
           SLNS+LLCGLR GAAILIMQKFDIV   +L+EKY+V+I P VPPI+LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND  +T RT+DKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVP 480
           DIG+ID+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DEQAGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  +TE++VK F+SKQV+FYKR+KRVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 VPN 544

BLAST of Tan0000114 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 4.2e-231
Identity = 394/543 (72.56%), Postives = 460/543 (84.71%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           M  +T Q  + IFRSKLPDI+IPN+LPLH Y F+N ++F SRPCLI+GA   +Y+Y DV+
Sbjct: 1   MEKDTKQV-DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L SR+VA+GLH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFD 180
           AKA++AK+IVT AC  ++VKD A EN VKI+C+D   EGCLHFSVL+ A+E   P V+  
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQ 180

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY H  DV+LCVLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIY 240

Query: 241 SLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDV 300
           SLNS+LLCGLR GAAILIMQKFDIVS L+L+++Y+V+I P VPPI+LAIAKSP  + YD+
Sbjct: 241 SLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 301 SSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA 360

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N +GEICIRGDQIMKGYLND E+T RT+DKEGWL+TG
Sbjct: 361 CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTG 420

Query: 421 DIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVP 480
           DIG+IDDDDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DEQAGEVP
Sbjct: 421 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  +TE++VK FISKQV+FYKR+KRVFFV+ IPK+PSGKILRK+LRAKLA+G
Sbjct: 481 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 LPN 542

BLAST of Tan0000114 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 7.2e-231
Identity = 389/540 (72.04%), Postives = 456/540 (84.44%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQLTS 63
           ET Q+ + IFRSKLPDI+IP +LPLH Y F+N ++F SRPCLI+GA   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDSDD 183
           ++AK+I+T +CF  +VKD A EN VK++C+D   EGCLHFS L+ +DE   P V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H  DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVSSV 303
           SILLCGLR GAAILIMQKFDI   L+L++KY+VSI P VPPI+LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPVAF 483
           FID+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M DEQAGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGAYN 543
           VV++NG A+TE++VK FISKQV+FYKR+KRVFFV T+PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Tan0000114 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 4.0e-229
Identity = 387/543 (71.27%), Postives = 453/543 (83.43%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           M +ET Q+ + IFRSKLPDI+IP +LPLH Y F+N ++F SRPCLIDGA   +Y+Y +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTSR+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFD 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD   EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H  DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDV 300
           SLNS+LLC LR GAAILIMQKFDI   L+L+ K++V+I P VPPI+LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVP 480
           DIGFIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M DEQAGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  +TE++VK FISKQV+FYKR+KRVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Tan0000114 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.2e-229
Identity = 387/543 (71.27%), Postives = 453/543 (83.43%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           M  ET Q+ + IFRSKLPDI+IP +LPLH Y F+N ++F SRPCLIDGA   +Y+Y +V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTSR+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFD 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD   EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H  DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDV 300
           SLNS+LLC LR GAAILIMQKFDI   L+L+ K++V+I P VPPI+LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVP 480
           DIGFIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M DEQAGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV++NG  +TE++VK FISKQV+FYKR+KRVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Tan0000114 vs. NCBI nr
Match: KAG6593348.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.4e-294
Identity = 512/542 (94.46%), Postives = 527/542 (97.23%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPN+LPLH YVFQNQ+KFGSRPCLI+GATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVD+ VE CLH+SVLSGADESH P VDF +
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVS LQLVEKY+VSIMPIVPPI LAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPELEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPAA+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Tan0000114 vs. NCBI nr
Match: XP_022959958.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.4e-294
Identity = 512/542 (94.46%), Postives = 527/542 (97.23%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPN+LPLH YVFQNQ+KFGSRPCLI+GATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVD+ VE CLH+SVLSGADESH P VDF +
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVS LQLVEKY+VSIMPIVPPI LAIAKSPD EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPAA+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFID+DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Tan0000114 vs. NCBI nr
Match: XP_022991872.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 1020.0 bits (2636), Expect = 7.5e-294
Identity = 509/542 (93.91%), Postives = 530/542 (97.79%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPN+LPLHEYVFQN +KFGSRPCLI+GATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L SRRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASY GA+MTAANPFYTAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDF VEGCLH+SVLSGADESH PPV+F +
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVSLL+L+EK++VSIMPIVPPI LAIAKSP+FEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AVLGQGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFIDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Tan0000114 vs. NCBI nr
Match: XP_023004761.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 1016.9 bits (2628), Expect = 6.4e-293
Identity = 508/542 (93.73%), Postives = 526/542 (97.05%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPN+LPLH YVFQNQ+K GSRPCLI+GATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+RRVASGLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVD+ VEGCLH+SVLSGADESH P VDF +
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVSLLQLVEKY+VSIMPIVPPI LAIAKSPD EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPAA+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGF+DD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDE+AGEVPV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDV QFIS+QVVFYKRLKRVFFVN IPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Tan0000114 vs. NCBI nr
Match: XP_023547628.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 508/542 (93.73%), Postives = 529/542 (97.60%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPN+LPLHEYVFQN +KFGSRPCLI+GATG V+SY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVHSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L SRRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFL ASY GA+MTAANPFYTAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDF VEGCLH+SVLSGADESH P V+F +
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVSLL+L+EK++VSIMPIVPPI LAIAKSP+FEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFIDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Tan0000114 vs. ExPASy TrEMBL
Match: A0A6J1H7E7 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460855 PE=4 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.1e-294
Identity = 512/542 (94.46%), Postives = 527/542 (97.23%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPN+LPLH YVFQNQ+KFGSRPCLI+GATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVD+ VE CLH+SVLSGADESH P VDF +
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVS LQLVEKY+VSIMPIVPPI LAIAKSPD EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPAA+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFID+DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of Tan0000114 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 1020.0 bits (2636), Expect = 3.6e-294
Identity = 509/542 (93.91%), Postives = 530/542 (97.79%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPN+LPLHEYVFQN +KFGSRPCLI+GATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L SRRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASY GA+MTAANPFYTAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDF VEGCLH+SVLSGADESH PPV+F +
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVSLL+L+EK++VSIMPIVPPI LAIAKSP+FEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AVLGQGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFIDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Tan0000114 vs. ExPASy TrEMBL
Match: A0A6J1KT15 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497966 PE=4 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.1e-293
Identity = 508/542 (93.73%), Postives = 526/542 (97.05%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPN+LPLH YVFQNQ+K GSRPCLI+GATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+RRVASGLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVD+ VEGCLH+SVLSGADESH P VDF +
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVSLLQLVEKY+VSIMPIVPPI LAIAKSPD EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPAA+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGF+DD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDE+AGEVPV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDV QFIS+QVVFYKRLKRVFFVN IPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Tan0000114 vs. ExPASy TrEMBL
Match: A0A6J1GMS9 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455820 PE=4 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 504/542 (92.99%), Postives = 527/542 (97.23%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPN+LPLHEYVFQN +KFGSRPCLI+GATG VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L SRRVA+GLHNLGIKK DVVMNLLPNSPEFVFTFL ASY GA+MTAANPFYTAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDF VEGCLH+SVLSGADESH P V+F +
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHG DVILCVLPFFH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+I+SLL+L+EK++VSIMPIVPPI LAIAKSP+FEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPDEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKANGGA+TEEDVKQFISKQVVFYKRLKRVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of Tan0000114 vs. ExPASy TrEMBL
Match: A0A6J1KVH9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497965 PE=4 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 494/542 (91.14%), Postives = 518/542 (95.57%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQ 60
           MA E NQT EFIFRSKLPDIHIPN+LPLHEY+FQN   +GSRPCLI+GATGHVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60

Query: 61  LTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESHAPPVDFDS 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVD+ VEGCLH+SVLSGADESH P VDF +
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVS 300
           LNSILLCGLRAGAAILIMQKF+IVSLL+LVEKY+ SI+PIVPPILLAIAKS +FEKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGK+LEDAVREKFP A+LGQGYGMTEAGPVLTMSLAFAKEPFPVK GAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLPANSAGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480
           IGFIDDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPDEQAGE+PV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVV ANGG +TEEDVKQFIS+QVVFYKRLKRVFFVN IPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Tan0000114 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 750.7 bits (1937), Expect = 7.9e-217
Identity = 371/543 (68.32%), Postives = 446/543 (82.14%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPN+L LH+Y+FQN ++F ++PCLI+G TGHVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAAN 125
           +A+  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDRVKDLA-ENGVKIVCVDFT-----VEGCLHFSVLSGADESHAPPVD-- 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D  
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 -FDSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFF 245
               DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+H  DVILCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEK 305
           HIY+LNSI+LCGLR GAAILIM KF+I  LL+L+++ +V++ P+VPPI+LAIAKS + EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAG 485
           HTGDIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKL 540
           EVPVAFVVK+    ++E+DVKQF+SKQVVFYKR+ +VFF  +IPKAPSGKILRK+LRAKL
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Tan0000114 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 745.7 bits (1924), Expect = 2.6e-215
Identity = 363/539 (67.35%), Postives = 451/539 (83.67%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQLTSRRVA 67
           +N+ IFRS+LPDI+IPN+LPLH+Y+F+N ++F ++PCLI+G TG VY+Y DV +TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  SGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           +GLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDFTV--EGCLHFSVLSGADESHAP--PVDFDSDDV 187
           LIVT + + D++K+L  +GV IV  D     E CL FS L+ ++E      P     +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY++  DVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVSSVR 307
           I+LC LR GA ILIM KF+I  LL+ +++ +V++  +VPPI+LAIAKSP+ EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTV 367
           ++KSG APLGKELEDA+  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  T+DK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPVAFV 487
           IDDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M +E AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKLASGAYN 543
           V++    ++E+++KQF+SKQVVFYKR+ +VFF ++IPKAPSGKILRK+LRA+LA+G  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of Tan0000114 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-200
Identity = 344/508 (67.72%), Postives = 414/508 (81.50%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPN+L LH+Y+FQN ++F ++PCLI+G TGHVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAAN 125
           +A+  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDRVKDLA-ENGVKIVCVDFT-----VEGCLHFSVLSGADESHAPPVD-- 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D  
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 -FDSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFF 245
               DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+H  DVILCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEK 305
           HIY+LNSI+LCGLR GAAILIM KF+I  LL+L+++ +V++ P+VPPI+LAIAKS + EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAG 485
           HTGDIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKANGGAMTEEDVKQFISKQV 505
           EVPVAFVVK+    ++E+DVKQF+SKQV
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Tan0000114 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 682.9 bits (1761), Expect = 2.0e-196
Identity = 331/546 (60.62%), Postives = 426/546 (78.02%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNYLPLHEYVFQNQAKFG----SRPCLIDGATGHVYSYDDV 63
           E   +++FIFRSKLPDI IPN+LPL +YVFQ  +  G    S  C+IDGATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTSRRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+A+G+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVKIVCVD---------FTVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHAPPVDFDSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVIL 243
           +         +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +   DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAK 303
           C LP FHIY+L++++L  +R GAA+LI+ +F++  +++L+++Y+V+++P+ PP++LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPDFEKYDVSSVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAK 363
           SP+ E+YD+SSVR++ SG A L KELEDAVR KFP A+ GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTV 423
            PF  K+GACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RT+
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGF+DDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDEQAGEVPVAFVVKANGGAMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRK 537
            DE A EVPVAFV ++ G  +TE+DVK +++KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Tan0000114 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 676.0 bits (1743), Expect = 2.5e-194
Identity = 334/527 (63.38%), Postives = 416/527 (78.94%), Query Frame = 0

Query: 12  IFRSKLPDIHIPNYLPLHEYVFQNQAKFGSRPCLIDGATGHVYSYDDVQLTSRRVASGLH 71
           IFRSKLPDI IPN+LPLH Y F+  +    +PCLI G+TG  Y+Y +  L  RRVASGL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVKIVCVDFTVEGCLHFSVLSGADESH--APPVDFDSDDVVALPYS 191
            + + D++K+L EN   I   + T E CL FS L   DE++     VD   DD  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY    DVILCVLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAILIMQKFDIVSLLQLVEKYRVSIMPIVPPILLAIAKSPDFEKYDVSSVRVLKSGG 311
           R+GA +L+M KF+I +LL L++++RV+I  +VPP+++A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVREKFPAAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P A+LGQGYGMTEAGPVL+MSL FAKEP P K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFIDDDDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  T+D+EGWLHTGDIG++D+DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPVAFVVKANGG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   DE AGEVPVAFVV++NG 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AMTEEDVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRAKL 537
            +TEEDVK++++KQVVFYKRL +VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560