Spg039967 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039967
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionglycosylphosphatidylinositol anchor attachment 1 protein-like
Locationscaffold10: 43144627 .. 43148809 (+)
RNA-Seq ExpressionSpg039967
SyntenySpg039967
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGATCGTATTTACTCATGATAATCATGTGCCACAAGGTATTGGACTCAGAGGGAAACCACCACAACCATTTAGCCTACAACGCTTAGTTGTGGACACCCTCAAATTCCCCAATTCTAGCCCCCTGCATCTCATCGATTTCATCACCAACTCCCAATTCTATAAAATCATAGCCTTGCTTCTAAACACTGAACCTCCAAAAAGATCCTAAGAACAAGTGATCTATCCTCAATGGGAGGAAGATCTCCAAACCAACTTCATCCATCACAAGAGACCAGGCACTACGAGCAAAATTACAATGCAGTAAAATATGATCCGCTATATATGCAGAAACACTTGACAGTACTCTTAACTGTGGCATTATATCTTCCCACAGGTTCTGCAAATACTATGCTTTCAGGTCAGGAAGTCTCAGAGGCGAACAACTTGGTGAAGGATCTGAAGGGTTTGAATTCAAAGTCAGGGTCAACAGTCTTGTATGTTCCTACTGCGCTGGTTTTATCCACTTTCTATTTTCCTTTAAAATATAATAGAAAAATTTTGATGCTTAACTCGTAGTGCCTATAATTTTGAGAATTACCACTTCACAATGTAAAATTTATCTACTTGATTTATGTTTTGAATGCCTTGACATTAAAATGGTCTTATGCACATCCATGCTTTTAAGGCATCTGTGCATTTTAACTCCGTGAACTATTTGACTAATTACAAGAATTTAAGTCAATCAATAGTAGGGCTGAACTTTCAAGTTCACTTCTATGCTGTAGTCCCATGAAAATGTCAAGGAATCATTAGCAAGCTCAATTGAGCCAATTGGATGAATATTTAAGTATGTTATCCGACTTTGGCTTTCAGTATGAACTTCTGAATGAGTCTAATATTGGCCTCCTAAATTGACAATCTCAAGAGGTTAAGAACTTAAGAGAAACAGGCAAGCTGAGGACAGTCCTTGTCTGTAAAGGTATTAGAAAATTAGTTCTAAAGTTCTCATCATGTATCAAATGAATGCCTTCATCAAATTGTGGTATATGGTATTGTTTTCTGATATGTGATGTTTGCGCTAGTTTTATTTTATCTATGACTATGTAGTCTGTGCATGTGGGTCAGCATGCCATTTTATGTTGGTCAAGAGGGATGTTTGTGCGTAGAGACGATCATAAACTTAATTGTGAGTAACTTTTTTCACTAATTATATTGATATTGTATACTTCATAGTTATCGATTTCATCTTCCACCTAGAAATTATCTATAGCATCTTTTTCACATTTCCATAAGCATAATCTTCTTCCTCCCCACCCAACCTATGATAAAGAAATTTGATTTTCTATTCATTTTTCAGTGGAAGCCAGCAGATTATAGCACAATATATATCAAACTTGGGTGCTGAAGTTAATTACCACAGGTTCCACCCGCTGTTTAGTCAGTTTCATCCACTGCACTTTTTTTCCAGCCCTGATTCTGGGATCATTCGGGACAATGTTAGTTGCATGACACAGGGTATTAACACTGTTGGCATAATAAGAGCACCACAAGCCGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAGGACTAGTCTTCATGAGACTCTGTCTCTTGGTATTGCTTATTCAGTGTTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACATTGTATGGCTTGTTGCAGATTCTCGATATGGGGAATATGCTGCAGTTTCAGCATGGCTTAGAGATTACCACACTCCTGCATTTGGTCGGTCCAGCATAATTAACACAGATGCTTGTACTGAGTCAAATGTCCTTTATGAATTAGAAGCAAACCATATGACAGAGAAAAGGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTTATTAAGGTTTCAAATAAAAGCGAACACTTTGAGGATAGTCTTAGTGTGTATGCTGAAGCATCTAATGGTCAAATGCCAAACCTGGACCTCATCAATATTGTGAATTATCTAGCAGTACATAGGCAAGGTTTTCGGATTAAGATTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGAAAATTTATCAGAAGCTTAAACCCTGAATGGAAGTTTGGCATGCCAGCTTCGGACTATGTTGATGGCACCGCCACACTTGCAAGTTCATTGTACTACCAGGTGGTTTACTCTATATTTCATTTTCTAAGAGATTGTTGTTTATGGTTTCGTTTAATGGACAAGAATAAGGAAGGATTTTTCATTTATGTTAAAAATAGAGATTGTTTTCTCTAAAATTATCTATGTTCTTCTTGCCATAGGCTGTGGGTATTCCTACTGGTCCCCATGGGGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATATTGCGAGGTGGCCGGTCAGTGCTGGTTTATGCCCCAATAATTTTATTACAGATCCTCCTTCCACCCCTCTACTTTTCAGGGCTCACTACATAAATAAATGTTTAAAACCAATGGTTTCACCCAATGGGGTTAGGTCAACATTAAACAATATTTTCAACTTTTTTATTATTTATCATTTCCCATTTTTTTTTCTTCGCAGTGAACACTGTCAATCTTCAATGATTAAATTATAATTTGAATATAATTTTAACGGGGTAAGAATTTTCTTTTTCCCTAAATTGAACTTTTGACTGAGTTTCTCTTTTTAGTTTCTAAATGAATAGATTTTACGTGTGAAATTCATCCAACTGAATTTATTGGCTAAGTATTCATTCAAGTGATTTGACGTGTGAAATTCATGCTTATGTATGGGAATGCGTTTAGATTTTGATGTATTAAATGATCAAGACATTCATAATTGAATTTGAAATACAAAATTGGCAATTTACCGCCCAGCTTGTAGTTAATTCTGCATGAAAGGAATGAAATTCTCCTGATCCTTTTGATTTGGCTAAGAATTTTATAGTTGGTTGTGCAGGCTAATTGAAGGAGTTGTACGGTCAGTAAACAACCTCCTGGAGAAGTTCCATCAGTCATTCTTTCTATACCTTATGGTATCCACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCTTTTGCACTCCTTGTTGCTCCACTACCAGCAGTTGCAGCTTCCCTCTATTCTTATGCCAATAACTTGAATTTAACCTTGGAAAAGGCTGAACCTGCAGTTCCTGCAAATGCTGACGATGTGCTTATTGTCTCTTTAAGGTCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTTATCTATGGGGTGCTGTTGTTTCCTTACTTCCATACTTCATCTGCCAAATACCTGGTTACAGTCCTACAACAAACACTATTATTTGGGCGTTGCTTTCACTGCTCACTCTACTAATTTTGTCAGTGATATTGGGCTCTCCACTTGGTTCCACCAAATCTTCAGAACAGCGGATTCAAGAGTGGGCTTTCTTGAAAGCAATGACAACCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATCAACTTTGCTACTGCAGAACTTGGAGCATTTTTGGTGGTATCCATGTGCTTGTTGGCACATCCTCTGAAGCTTGATTTAATGGCTGGGAGCTTTAAAGCTCTTTCAAGGGCAGCCTGCAACATTGTTTTGGGATCCATAGCTTTTCCACCTGTTACATTCTTTTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATCTACATATCGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCCACGTTCCTCTACTTAGGTATGGTTCACCTGCCATGCTGGGTATTATGTACACAAATTTTATTTCATCATTGTTAATGTAACTTGTTTAGTTTATTCCATGACATTTGGTTCGACCAGCCAATTAGGAGATTCTTAGAGTTACCCAATTTTTCTTTTGCTCGAATAGCTAATTTATAAAACTTAGAAATAGCTTTCAACTGTATTGGAACAAGTTACACGGTCATCACTTGTATGGTAGCTTCTGTAAGCAGGCCTTAAAGCCTACTTTGTTACAGTCGTTTATAGTTGTAGAATTAATACAGTAAACTTTTACTCTCTTCGAATTAATTGGACAATTTACCTTCTCT

mRNA sequence

ATGAGGATCGTATTTACTCATGATAATCATGTGCCACAAGGTTCTGCAAATACTATGCTTTCAGGTCAGGAAGTCTCAGAGGCGAACAACTTGGTGAAGGATCTGAAGGGTTTGAATTCAAAGTCAGGGTCAACAGTCTTGTATGTTCCTACTGCGCTGTTTTATTTTATCTATGACTATGTAGTCTGTGCATGTGGGTCAGCATGCCATTTTATTGGAAGCCAGCAGATTATAGCACAATATATATCAAACTTGGGTGCTGAAGTTAATTACCACAGGTTCCACCCGCTGTTTAGTCAGTTTCATCCACTGCACTTTTTTTCCAGCCCTGATTCTGGGATCATTCGGGACAATGTTAGTTGCATGACACAGGGTATTAACACTGTTGGCATAATAAGAGCACCACAAGCCGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAGGACTAGTCTTCATGAGACTCTGTCTCTTGGTATTGCTTATTCAGTGTTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACATTGTATGGCTTGTTGCAGATTCTCGATATGGGGAATATGCTGCAGTTTCAGCATGGCTTAGAGATTACCACACTCCTGCATTTGGTCGGTCCAGCATAATTAACACAGATGCTTGTACTGAGTCAAATGTCCTTTATGAATTAGAAGCAAACCATATGACAGAGAAAAGGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTTATTAAGGTTTCAAATAAAAGCGAACACTTTGAGGATAGTCTTAGTGTGTATGCTGAAGCATCTAATGGTCAAATGCCAAACCTGGACCTCATCAATATTGTGAATTATCTAGCAGTACATAGGCAAGGTTTTCGGATTAAGATTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGAAAATTTATCAGAAGCTTAAACCCTGAATGGAAGTTTGGCATGCCAGCTTCGGACTATGTTGATGGCACCGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTCCCCATGGGGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATATTGCGAGGTGGCCGGCTAATTGAAGGAGTTGTACGGTCAGTAAACAACCTCCTGGAGAAGTTCCATCAGTCATTCTTTCTATACCTTATGGTATCCACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCTTTTGCACTCCTTGTTGCTCCACTACCAGCAGTTGCAGCTTCCCTCTATTCTTATGCCAATAACTTGAATTTAACCTTGGAAAAGGCTGAACCTGCAGTTCCTGCAAATGCTGACGATGTGCTTATTGTCTCTTTAAGGTCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTTATCTATGGGGTGCTGTTGTTTCCTTACTTCCATACTTCATCTGCCAAATACCTGGTTACAGTCCTACAACAAACACTATTATTTGGGCGTTGCTTTCACTGCTCACTCTACTAATTTTGTCAGTGATATTGGGCTCTCCACTTGGTTCCACCAAATCTTCAGAACAGCGGATTCAAGAGTGGGCTTTCTTGAAAGCAATGACAACCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATCAACTTTGCTACTGCAGAACTTGGAGCATTTTTGGTGGTATCCATGTGCTTGTTGGCACATCCTCTGAAGCTTGATTTAATGGCTGGGAGCTTTAAAGCTCTTTCAAGGGCAGCCTGCAACATTGTTTTGGGATCCATAGCTTTTCCACCTGTTACATTCTTTTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATCTACATATCGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCCACGTTCCTCTACTTAGGTATGGTTCACCTGCCATGCTGGGTATTATGTACACAAATTTTATTTCATCATTGTTAA

Coding sequence (CDS)

ATGAGGATCGTATTTACTCATGATAATCATGTGCCACAAGGTTCTGCAAATACTATGCTTTCAGGTCAGGAAGTCTCAGAGGCGAACAACTTGGTGAAGGATCTGAAGGGTTTGAATTCAAAGTCAGGGTCAACAGTCTTGTATGTTCCTACTGCGCTGTTTTATTTTATCTATGACTATGTAGTCTGTGCATGTGGGTCAGCATGCCATTTTATTGGAAGCCAGCAGATTATAGCACAATATATATCAAACTTGGGTGCTGAAGTTAATTACCACAGGTTCCACCCGCTGTTTAGTCAGTTTCATCCACTGCACTTTTTTTCCAGCCCTGATTCTGGGATCATTCGGGACAATGTTAGTTGCATGACACAGGGTATTAACACTGTTGGCATAATAAGAGCACCACAAGCCGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAGGACTAGTCTTCATGAGACTCTGTCTCTTGGTATTGCTTATTCAGTGTTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACATTGTATGGCTTGTTGCAGATTCTCGATATGGGGAATATGCTGCAGTTTCAGCATGGCTTAGAGATTACCACACTCCTGCATTTGGTCGGTCCAGCATAATTAACACAGATGCTTGTACTGAGTCAAATGTCCTTTATGAATTAGAAGCAAACCATATGACAGAGAAAAGGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTTATTAAGGTTTCAAATAAAAGCGAACACTTTGAGGATAGTCTTAGTGTGTATGCTGAAGCATCTAATGGTCAAATGCCAAACCTGGACCTCATCAATATTGTGAATTATCTAGCAGTACATAGGCAAGGTTTTCGGATTAAGATTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGAAAATTTATCAGAAGCTTAAACCCTGAATGGAAGTTTGGCATGCCAGCTTCGGACTATGTTGATGGCACCGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTCCCCATGGGGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATATTGCGAGGTGGCCGGCTAATTGAAGGAGTTGTACGGTCAGTAAACAACCTCCTGGAGAAGTTCCATCAGTCATTCTTTCTATACCTTATGGTATCCACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCTTTTGCACTCCTTGTTGCTCCACTACCAGCAGTTGCAGCTTCCCTCTATTCTTATGCCAATAACTTGAATTTAACCTTGGAAAAGGCTGAACCTGCAGTTCCTGCAAATGCTGACGATGTGCTTATTGTCTCTTTAAGGTCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTTATCTATGGGGTGCTGTTGTTTCCTTACTTCCATACTTCATCTGCCAAATACCTGGTTACAGTCCTACAACAAACACTATTATTTGGGCGTTGCTTTCACTGCTCACTCTACTAATTTTGTCAGTGATATTGGGCTCTCCACTTGGTTCCACCAAATCTTCAGAACAGCGGATTCAAGAGTGGGCTTTCTTGAAAGCAATGACAACCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATCAACTTTGCTACTGCAGAACTTGGAGCATTTTTGGTGGTATCCATGTGCTTGTTGGCACATCCTCTGAAGCTTGATTTAATGGCTGGGAGCTTTAAAGCTCTTTCAAGGGCAGCCTGCAACATTGTTTTGGGATCCATAGCTTTTCCACCTGTTACATTCTTTTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATCTACATATCGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCCACGTTCCTCTACTTAGGTATGGTTCACCTGCCATGCTGGGTATTATGTACACAAATTTTATTTCATCATTGTTAA

Protein sequence

MRIVFTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCACGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVLIVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILSVILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILFHHC
Homology
BLAST of Spg039967 vs. NCBI nr
Match: XP_023550992.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 612/689 (88.82%), Postives = 636/689 (92.31%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKD+KGLNSKSGST+                  
Sbjct: 7   YISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTI------------------ 66

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  SQFHPLHFFSSPDSGI++DNVSCM+Q
Sbjct: 67  -------FGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMSQ 126

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 127 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVW 186

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRSS+I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 187 LAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFK 246

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGFRIKIEK
Sbjct: 247 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEK 306

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE FESIGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 307 FWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 366

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 367 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 426

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEK EPA PAN DDVL
Sbjct: 427 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKIEPAAPANPDDVL 486

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKWLNAAKRVFVV+LWGAVVSLLPYFICQIPGYSPTTN+IIWALLSLLTLLILS
Sbjct: 487 IVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYSPTTNSIIWALLSLLTLLILS 546

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 547 VILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 606

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+AG+FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 607 HPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 666

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 667 AWNSATFLYLGMVHLPCWVLCTQILLHPC 670

BLAST of Spg039967 vs. NCBI nr
Match: XP_023550991.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 612/689 (88.82%), Postives = 636/689 (92.31%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKD+KGLNSKSGST+                  
Sbjct: 51  YISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTI------------------ 110

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  SQFHPLHFFSSPDSGI++DNVSCM+Q
Sbjct: 111 -------FGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMSQ 170

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 171 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVW 230

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRSS+I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 231 LAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFK 290

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGFRIKIEK
Sbjct: 291 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEK 350

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE FESIGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 351 FWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 410

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 411 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 470

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEK EPA PAN DDVL
Sbjct: 471 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKIEPAAPANPDDVL 530

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKWLNAAKRVFVV+LWGAVVSLLPYFICQIPGYSPTTN+IIWALLSLLTLLILS
Sbjct: 531 IVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYSPTTNSIIWALLSLLTLLILS 590

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 591 VILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 650

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+AG+FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 651 HPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 710

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 711 AWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Spg039967 vs. NCBI nr
Match: KAG7016207.1 (Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 606/689 (87.95%), Postives = 634/689 (92.02%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKD+KGLNSKSGST+                  
Sbjct: 51  YISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTI------------------ 110

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  SQFHPLHFFSSPDSGI++DNVSCMTQ
Sbjct: 111 -------FGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQ 170

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 171 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVW 230

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRS++I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 231 LAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFK 290

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAAL+IKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGFRIKIEK
Sbjct: 291 RAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEK 350

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE+FE+IGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 351 FWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 410

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 411 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 470

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEK EPA PAN DD L
Sbjct: 471 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKIEPAAPANPDDEL 530

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSW+WLNAAKRVFVV+LWGAVVSLLPYFICQIP YSPTTN+IIWALLSLLTLLILS
Sbjct: 531 IVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICQIPDYSPTTNSIIWALLSLLTLLILS 590

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 591 VILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 650

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+A +FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 651 HPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 710

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 711 AWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Spg039967 vs. NCBI nr
Match: XP_038891215.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X2 [Benincasa hispida])

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 608/689 (88.24%), Postives = 629/689 (91.29%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSG EVSEANNLVKDLKGLNSK GS +                  
Sbjct: 7   YISENALMPGSANTMLSGWEVSEANNLVKDLKGLNSKPGSAI------------------ 66

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYIS LGA+VNYHRFHP  SQFHPLHFFSSPDSGI+R NVSCMTQ
Sbjct: 67  -------FGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHFFSSPDSGIVRGNVSCMTQ 126

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLH+TLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 127 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHDTLSLGIAYSIFSLLTQVTWLAKDIVW 186

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           LVADSRYGEY AVSAWLRDYH P FGRSS+I+TDACTE+NVLYE+EANH TEK +LDDFK
Sbjct: 187 LVADSRYGEYDAVSAWLRDYHAPVFGRSSVIDTDACTETNVLYEVEANHATEKINLDDFK 246

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK
Sbjct: 247 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 306

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLKVLGEVFESIGK IRSLN EWKFGMPASDYVDGTATLASSLYYQAVGIPT
Sbjct: 307 FWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGMPASDYVDGTATLASSLYYQAVGIPT 366

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           G HGAFRDYQIDAITVEMSPKFSS VKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 367 GSHGAFRDYQIDAITVEMSPKFSSAVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 426

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAA+LYSYANN+NLTLEK EP  PAN D+ L
Sbjct: 427 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAAALYSYANNMNLTLEKVEPPAPANPDNEL 486

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKWLNAAKRVFVV+LWGAVVSLLPYFICQIPGYSPT N+IIWALLSLL LLILS
Sbjct: 487 IVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYSPTANSIIWALLSLLALLILS 546

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPLGSTKS EQRIQEWAFLKAMTTS AFI LCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 547 VILGSPLGSTKSYEQRIQEWAFLKAMTTSTAFISLCLMSVINFATAELGAFLVVSMCLLA 606

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDLMAGSFKALSRAACNIVLG IAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW
Sbjct: 607 HPLKLDLMAGSFKALSRAACNIVLGFIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 666

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 667 AWNSATFLYLGMVHLPCWVLCTQILLHPC 670

BLAST of Spg039967 vs. NCBI nr
Match: XP_038891214.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Benincasa hispida])

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 608/689 (88.24%), Postives = 629/689 (91.29%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSG EVSEANNLVKDLKGLNSK GS +                  
Sbjct: 51  YISENALMPGSANTMLSGWEVSEANNLVKDLKGLNSKPGSAI------------------ 110

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYIS LGA+VNYHRFHP  SQFHPLHFFSSPDSGI+R NVSCMTQ
Sbjct: 111 -------FGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHFFSSPDSGIVRGNVSCMTQ 170

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLH+TLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 171 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHDTLSLGIAYSIFSLLTQVTWLAKDIVW 230

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           LVADSRYGEY AVSAWLRDYH P FGRSS+I+TDACTE+NVLYE+EANH TEK +LDDFK
Sbjct: 231 LVADSRYGEYDAVSAWLRDYHAPVFGRSSVIDTDACTETNVLYEVEANHATEKINLDDFK 290

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK
Sbjct: 291 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 350

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLKVLGEVFESIGK IRSLN EWKFGMPASDYVDGTATLASSLYYQAVGIPT
Sbjct: 351 FWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGMPASDYVDGTATLASSLYYQAVGIPT 410

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           G HGAFRDYQIDAITVEMSPKFSS VKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 411 GSHGAFRDYQIDAITVEMSPKFSSAVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 470

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAA+LYSYANN+NLTLEK EP  PAN D+ L
Sbjct: 471 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAAALYSYANNMNLTLEKVEPPAPANPDNEL 530

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKWLNAAKRVFVV+LWGAVVSLLPYFICQIPGYSPT N+IIWALLSLL LLILS
Sbjct: 531 IVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYSPTANSIIWALLSLLALLILS 590

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPLGSTKS EQRIQEWAFLKAMTTS AFI LCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 591 VILGSPLGSTKSYEQRIQEWAFLKAMTTSTAFISLCLMSVINFATAELGAFLVVSMCLLA 650

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDLMAGSFKALSRAACNIVLG IAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW
Sbjct: 651 HPLKLDLMAGSFKALSRAACNIVLGFIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 710

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 711 AWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Spg039967 vs. ExPASy Swiss-Prot
Match: Q9WTK3 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=10090 GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 112.8 bits (281), Expect = 1.5e-23
Identity = 142/578 (24.57%), Postives = 237/578 (41.00%), Query Frame = 0

Query: 122 MTQGINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKD 181
           M  G N  GI+RAP++   E++VL  P  P  T+   + ++G+  ++ +      + AKD
Sbjct: 123 MVSGTNVYGILRAPRSASTESLVLTVPCGPDATN---SQAVGLLLALAAHFRGQIYWAKD 182

Query: 182 IVWLVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLD 241
           I++LV D    +     AWL  YH         IN      S +                
Sbjct: 183 IIFLVTDH---DLLGTEAWLEAYHD--------INVTGIQSSPLQ--------------- 242

Query: 242 DFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIK 301
              RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N+       + G    
Sbjct: 243 --GRAGAIQAAVALELSS---DVVTSLDVTVEGLNGQLPNLDLLNLFQTFC-QKGGLLCT 302

Query: 302 IEKFWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVG 361
           ++    L    W  + G                          + G  TL   +  QA G
Sbjct: 303 LQ--GKLQPQDWTSLEGP-------------------------LQGLQTLLLMVLRQASG 362

Query: 362 IPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRG-GRLIEGVVRSVNNLLEKFH 421
            P GPHG F  Y ++A+T+     F      R+ ++ L   G+ +EG+ R +N+LLE+ H
Sbjct: 363 RPHGPHGLFLRYGVEALTLRGINSF------RQYKYDLATVGKALEGMFRKLNHLLERLH 422

Query: 422 QSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANA 481
           QSFF YL+ + S+FVS+G+YM A   L+  L   A  L+   +   +  E+A  A P+  
Sbjct: 423 QSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKA-PSPG 482

Query: 482 DDVLIVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTL 541
             +L         L A   + +    G  +  LP     +             +L+LL +
Sbjct: 483 TPLLPTQGVGLASLTAP--LLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAI 542

Query: 542 LILSVILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSM 601
            +  + L        +S+   + W  LK +      + L  ++++NF+   L A  +V  
Sbjct: 543 YVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPA 602

Query: 602 CLLAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWM 661
             LA P     +  +   ++  A  +  GS+       FL++  L+    L + + W   
Sbjct: 603 AALAKPHGPRTLYAALLVVTSPAVTL-FGSL-------FLWRELLE--VPLSLAEGWQLF 619

Query: 662 ETL--------WAWNSATFLYLGMVHLPCWVLCTQILF 691
            T         + + +  F  L +   PCW+L   +LF
Sbjct: 663 LTALAQGVLEHYTYGALLFPILALGLYPCWLLFWNVLF 619

BLAST of Spg039967 vs. ExPASy Swiss-Prot
Match: O43292 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 1.3e-22
Identity = 148/586 (25.26%), Postives = 249/586 (42.49%), Query Frame = 0

Query: 122 MTQGINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKD 181
           M  G N  GI+RAP+A   E++VL  P     T+   + ++G+  ++ +      + AKD
Sbjct: 123 MVSGTNVYGILRAPRAASTESLVLTVPCGSDSTN---SQAVGLLLALAAHFRGQIYWAKD 182

Query: 182 IVWLVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLD 241
           IV+LV +    +     AWL  YH                           ++T  +S  
Sbjct: 183 IVFLVTEH---DLLGTEAWLEAYHDV-------------------------NVTGMQSSP 242

Query: 242 DFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIK 301
              RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N+          F+  
Sbjct: 243 LQGRAGAIQAAVALELSS---DVVTSLDVAVEGLNGQLPNLDLLNL----------FQTF 302

Query: 302 IEKFWPLLDCKWLKVLGEVFESIGKFIRSLNPE-WKFGMPASDYVDGTATLASSLYYQAV 361
            +K   L   +            GK    L PE W         + G  TL   +  QA 
Sbjct: 303 CQKGGLLCTLQ------------GK----LQPEDW---TSLDGPLQGLQTLLLMVLRQAS 362

Query: 362 GIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEF-ILRGGRLIEGVVRSVNNLLEKF 421
           G P G HG F  Y+++A+T+     F      R+ ++ ++  G+ +EG+ R +N+LLE+ 
Sbjct: 363 GRPHGSHGLFLRYRVEALTLRGINSF------RQYKYDLVAVGKALEGMFRKLNHLLERL 422

Query: 422 HQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEK----AEPA 481
           HQSFFLYL+   S+FVS+G+YM A   L+  L   A  L+   +   + LE+      P+
Sbjct: 423 HQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPS 482

Query: 482 VP-ANADDVLIVSLRSWKWLNAAK--RVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIW 541
           VP   +  V + SL +   ++ A    ++V+ + G  V+   + + +             
Sbjct: 483 VPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEA---------- 542

Query: 542 ALLSLLTLLILSVILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAEL 601
            +L+LL +    + L        S++   + W  LK +      + L  +++ NF+   L
Sbjct: 543 VVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFL 602

Query: 602 GAFLVVSMCLLAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLH 661
            A  +V    LA P     +  +   L+  A  + LGS+       FL++   +    L 
Sbjct: 603 LATTMVPTAALAKPHGPRTLYAALLVLTSPAATL-LGSL-------FLWRELQEA--PLS 619

Query: 662 IGDFWNWMETLWA--------WNSATFLYLGMVHLPCWVLCTQILF 691
           + + W       A        + +  F  L +   PCW+L   +LF
Sbjct: 663 LAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLF 619

BLAST of Spg039967 vs. ExPASy Swiss-Prot
Match: Q9US48 (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gaa1 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.6e-15
Identity = 124/552 (22.46%), Postives = 216/552 (39.13%), Query Frame = 0

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           G N +  +RAP+ D  E+++L  P+       +    + +A S+       +  +KDI+ 
Sbjct: 123 GSNFITTLRAPRGDATESLLLCVPWKD-HIGQYNEAGVALAISLLKYFQGWSLWSKDIIL 182

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           ++ D                  P +G SS + +              +  T   S    K
Sbjct: 183 VIFDD-----------------PVYGPSSFLTS------------YFDQTTPYISYTPLK 242

Query: 245 -RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIE 304
            R+G++ A L +++   +E+  D L V  +A+NGQ+PNLDL N ++ + +    + ++++
Sbjct: 243 IRSGSIQAGLSLELVT-TENNSDVLEVLYQATNGQLPNLDLFNTISRIFMQHFNYPLRLQ 302

Query: 305 KFWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIP 364
                         G  F +                  S Y     +L   +  QAV   
Sbjct: 303 --------------GYDFHA---------------NSGSSYTSRLKSLWMGMLTQAVSNV 362

Query: 365 TGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEF---ILRGGRLIEGVVRSVNNLLEKFH 424
           T  H  F  Y+ID +T+ M  K         D F   + R G+ IE   RS+NNLLE  H
Sbjct: 363 TSAHALFPQYRIDMLTLRMKVK---------DPFSFDMFRFGQAIESTFRSLNNLLEHLH 422

Query: 425 QSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANA 484
           QSFF Y ++    F+S+G YM +  +L     A +  L +Y +                 
Sbjct: 423 QSFFFYFILDHLHFISIGNYMPSILIL-----AASFMLGAYRH----------------- 482

Query: 485 DDVLIVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPG-YSPTTNTIIWALLSLLT 544
                       W+N  K+   + LW      L    C I   Y  +++T I   + L  
Sbjct: 483 ------------WINHEKK---IDLWRPFSFWLFSIFCTIAAYYLVSSSTKITVFIFLYL 542

Query: 545 LLILSVILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVS 604
           +L    I+ S   +++ +E  +      K++  S       ++S +NF+ + + A L+V 
Sbjct: 543 MLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFIS-------VVSTLNFSLSFVVAILLVP 543

Query: 605 MCLLAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTF---FLFKAALQGFDNLHIGDF 664
           +  +           SF+   R        S+ F  +T+   F+F  +L    N  +  F
Sbjct: 603 LQFI-----------SFRFNRRL-------SLLFAVLTYFSTFIFLCSLSKILNGPLVPF 543

Query: 665 WNWMETLWAWNS 669
           W W +    +NS
Sbjct: 663 WLWAKEYELFNS 543

BLAST of Spg039967 vs. ExPASy Swiss-Prot
Match: P39012 (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAA1 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.2e-13
Identity = 91/349 (26.07%), Postives = 152/349 (43.55%), Query Frame = 0

Query: 125 GINTVGIIRAPQADGKEAIVLVTP-YNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIV 184
           G    G++ AP+ DG EA+VL  P +N          +LG++ + F     V W +K+I+
Sbjct: 117 GETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPV-W-SKNII 176

Query: 185 WLVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDF 244
            + +++     AA+ +W+  YHT                                SLD  
Sbjct: 177 VVFSEN---PRAALRSWVEAYHT--------------------------------SLD-- 236

Query: 245 KRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIE 304
              G++ AA+V+  S+  + FE  + +  +  NG++PNLDL+NI   +  H +G ++ + 
Sbjct: 237 LTGGSIEAAVVLDYSSTEDFFE-YVEISYDGLNGELPNLDLVNIAISITEH-EGMKVSLH 296

Query: 305 KFWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGT-----ATLASSLYYQ 364
                                             G+P+    +         L   +   
Sbjct: 297 ----------------------------------GLPSDQLTNNNFWSRLKILCLGIRDW 356

Query: 365 AVGIPTGPHG--AFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLL 424
           A+     PHG  AF  ++I ++T++     +SG        I   GR+ E + RS+NNLL
Sbjct: 357 ALSGVKKPHGNEAFSGWRIQSVTLKAHG--NSG------HDITTFGRIPEAMFRSINNLL 379

Query: 425 EKFHQSFFLYLMVSTSKFVSVGVYM-IAFALLVAPLPAVAASLYSYANN 465
           EKFHQSFF YL+++  +FVS+  Y+  A AL +A      +SL ++ NN
Sbjct: 417 EKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIA---FAISSLNAFINN 379

BLAST of Spg039967 vs. ExPASy TrEMBL
Match: A0A6J1JZW5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 608/689 (88.24%), Postives = 633/689 (91.87%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKDLKGLNSKSGST+                  
Sbjct: 7   YISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTI------------------ 66

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  SQFHPLHFFSSPDSGI++DNVSCMTQ
Sbjct: 67  -------FGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQ 126

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQV+WLAKDIVW
Sbjct: 127 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVW 186

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRS++I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 187 LAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFK 246

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGFRIKIEK
Sbjct: 247 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEK 306

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE FESIGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 307 FWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 366

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           G HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 367 GSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 426

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN NLTLEK EPA PAN+DD L
Sbjct: 427 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDEL 486

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKWLNAAKRVFVV+LWGAVVSLLPYFI QIPGYSPTTN+IIWALLSLLTLLILS
Sbjct: 487 IVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILS 546

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 547 VILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 606

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+AG+FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 607 HPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 666

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 667 AWNSATFLYLGMVHLPCWVLCTQILLHPC 670

BLAST of Spg039967 vs. ExPASy TrEMBL
Match: A0A6J1K1W5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 608/689 (88.24%), Postives = 633/689 (91.87%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKDLKGLNSKSGST+                  
Sbjct: 51  YISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTI------------------ 110

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  SQFHPLHFFSSPDSGI++DNVSCMTQ
Sbjct: 111 -------FGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQ 170

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQV+WLAKDIVW
Sbjct: 171 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVW 230

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRS++I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 231 LAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFK 290

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGFRIKIEK
Sbjct: 291 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEK 350

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE FESIGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 351 FWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 410

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           G HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 411 GSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 470

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN NLTLEK EPA PAN+DD L
Sbjct: 471 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDEL 530

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKWLNAAKRVFVV+LWGAVVSLLPYFI QIPGYSPTTN+IIWALLSLLTLLILS
Sbjct: 531 IVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILS 590

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 591 VILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 650

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+AG+FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 651 HPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 710

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 711 AWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Spg039967 vs. ExPASy TrEMBL
Match: A0A6J1FHQ4 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 605/689 (87.81%), Postives = 633/689 (91.87%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKD+KGLNSKSGST+                  
Sbjct: 51  YISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTI------------------ 110

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  S+FHPLHFFSSPDSGI++DNVSCMTQ
Sbjct: 111 -------FGSQQILAQYISNLGAEVNYHRFQPQSSRFHPLHFFSSPDSGIVQDNVSCMTQ 170

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 171 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVW 230

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRSS+I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 231 LAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFK 290

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGF+IKIEK
Sbjct: 291 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFQIKIEK 350

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE+FE+IGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 351 FWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 410

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 411 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 470

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN NLTLEK EPA PAN DD L
Sbjct: 471 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANPDDEL 530

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSW+WLNAAKRVFVV+LWGAVVSLLPYFIC IP YSPTTN+IIWALLSLLTLLILS
Sbjct: 531 IVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICPIPDYSPTTNSIIWALLSLLTLLILS 590

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 591 VILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 650

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+AG+FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 651 HPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 710

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 711 AWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Spg039967 vs. ExPASy TrEMBL
Match: A0A6J1FMH6 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 605/689 (87.81%), Postives = 633/689 (91.87%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVSEAN+LVKD+KGLNSKSGST+                  
Sbjct: 7   YISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTI------------------ 66

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQQI+AQYISNLGAEVNYHRF P  S+FHPLHFFSSPDSGI++DNVSCMTQ
Sbjct: 67  -------FGSQQILAQYISNLGAEVNYHRFQPQSSRFHPLHFFSSPDSGIVQDNVSCMTQ 126

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINTVGIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYS+FSLLTQVTWLAKDIVW
Sbjct: 127 GINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVW 186

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L ADSRYGEYAAVSAWLRDYHTP+FGRSS+I+T  C E+NVLYE EANH+TEK SLDDFK
Sbjct: 187 LAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFK 246

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLA HRQGF+IKIEK
Sbjct: 247 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFQIKIEK 306

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           FWPLLDCKWLK LGE+FE+IGKFIRSLNPEWKFGM ASDYVDGTATLASSLYYQAVGIPT
Sbjct: 307 FWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPT 366

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 367 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 426

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN NLTLEK EPA PAN DD L
Sbjct: 427 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANPDDEL 486

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSW+WLNAAKRVFVV+LWGAVVSLLPYFIC IP YSPTTN+IIWALLSLLTLLILS
Sbjct: 487 IVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICPIPDYSPTTNSIIWALLSLLTLLILS 546

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           VILGSPL STKS EQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA
Sbjct: 547 VILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 606

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+AG+FKALSRAA N+VLG IAFPPVTFFLFKAALQGFDNLH+GDFWNWMETLW
Sbjct: 607 HPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLW 666

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCTQIL H C
Sbjct: 667 AWNSATFLYLGMVHLPCWVLCTQILLHPC 670

BLAST of Spg039967 vs. ExPASy TrEMBL
Match: A0A6J1H9W9 (glycosylphosphatidylinositol anchor attachment 1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111460940 PE=4 SV=1)

HSP 1 Score: 1175.6 bits (3040), Expect = 0.0e+00
Identity = 596/689 (86.50%), Postives = 627/689 (91.00%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSANTMLSGQEVS++NNLVKDLK LNS+ GSTV                  
Sbjct: 51  YISENALMPGSANTMLSGQEVSDSNNLVKDLKSLNSQPGSTV------------------ 110

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                   GSQ+IIAQYI NLGAEVNYHRFHP  SQFHP+HFFSSPD GIIRDNVSC+TQ
Sbjct: 111 -------FGSQKIIAQYILNLGAEVNYHRFHPQLSQFHPVHFFSSPDPGIIRDNVSCITQ 170

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           GINT+GIIRAPQADGKEAIVLVTPYNPV+TSLHETLSLGIAYSVFSLL QVTWLAKDIVW
Sbjct: 171 GINTIGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSVFSLLAQVTWLAKDIVW 230

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           L+ADSRYGEYAAVSAWLRDYHTPAFGRS +I+TDAC E+NVLYE EANHMTEK SL DFK
Sbjct: 231 LIADSRYGEYAAVSAWLRDYHTPAFGRSIMIDTDACPETNVLYEFEANHMTEKMSLGDFK 290

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQMPNLDLINIVNYLAV+RQGFRIKIEK
Sbjct: 291 RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVYRQGFRIKIEK 350

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
           F PLLDCKWLKVLGEVFESIGKFIRS+NPEWKFGMPASDYVDGTATLASSLYYQAVGIPT
Sbjct: 351 FLPLLDCKWLKVLGEVFESIGKFIRSMNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 410

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           G HGAFRD+QIDAITVEMSPKF SGV VR DEFILRGGRLIEGVVRSVNNLLEKFHQSFF
Sbjct: 411 GSHGAFRDFQIDAITVEMSPKFPSGVNVRHDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 470

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLT+EK EPA  AN+DD L
Sbjct: 471 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTVEKVEPAASANSDDEL 530

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPGYSPTTNTIIWALLSLLTLLILS 544
           IVSLRSWKW+NAAKRVFVV++WGAVVSLLPYFICQIPGYSPTT +IIWALLSLLTLLILS
Sbjct: 531 IVSLRSWKWVNAAKRVFVVHVWGAVVSLLPYFICQIPGYSPTTRSIIWALLSLLTLLILS 590

Query: 545 VILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLA 604
           V+L SPL STKSSEQ+IQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLL 
Sbjct: 591 VVLCSPLRSTKSSEQQIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLV 650

Query: 605 HPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLW 664
           HPLKLDL+ GSFKALSRAACN+VLG IAFPPVTFFLFKAALQ  D+LHIGDFWNWMETLW
Sbjct: 651 HPLKLDLVPGSFKALSRAACNLVLGFIAFPPVTFFLFKAALQSLDDLHIGDFWNWMETLW 710

Query: 665 AWNSATFLYLGMVHLPCWVLCTQILFHHC 694
           AWNSATFLYLGMVHLPCWVLCT+IL H C
Sbjct: 711 AWNSATFLYLGMVHLPCWVLCTEILLHSC 714

BLAST of Spg039967 vs. TAIR 10
Match: AT5G19130.2 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 714.5 bits (1843), Expect = 8.1e-206
Identity = 375/690 (54.35%), Postives = 483/690 (70.00%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSA +MLS ++VS+ + LVKD+K         V                  
Sbjct: 63  YISENALMPGSARSMLSNRDVSDGSKLVKDIKNFRLNHEGQV------------------ 122

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                     Q++I +Y+S++GAEV+Y +FHP  +QFHPLHFFS PDS  + +NVSC + 
Sbjct: 123 ----------QKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASY 182

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           G+N  GIIRAP+ DGKE+IVLVTPY+ +    +E LSLGI  S+FSLL++VTWL+KDI+W
Sbjct: 183 GVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVSSLFSLLSRVTWLSKDIIW 242

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           LVADSRYG+Y  V+AWL +YH+P+F  S ++  D                 E+ + D+F+
Sbjct: 243 LVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD-----------------EQNTADNFR 302

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALV+KV  +SE FED+LS+YAEASNGQMPNLDLIN+VNYLAVHRQGF +K+EK
Sbjct: 303 RAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEK 362

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
              LL   WLK+ GE+FE++GK    LNP+W FG+PA+DY++G+ATLASSLY QA+GIPT
Sbjct: 363 VVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPT 422

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQ+DAIT+++SP+F    K R+ +F LRG RL+EG +RSVNNLLEKFHQSFF
Sbjct: 423 GPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFF 482

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LY++ S SKF+SVGVYMIAFALLVAPLP VAASLY      +LT     P          
Sbjct: 483 LYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLY-IDGCKSLTNSTHNPT--------- 542

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPG-YSPTTNTIIWALLSLLTLLIL 544
             + +SWKWL+AAK+VF ++L G +V+LLPYFICQ+PG +SPT  +I+W   S   LLI 
Sbjct: 543 -ENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLIT 602

Query: 545 SVILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLL 604
            V +  P  S  SS      WA LK++T SAAFIGLCLMS+INFATAE+GA L+V  CL+
Sbjct: 603 FVTM--PGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLM 662

Query: 605 AHPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAAL-QGFDNLHI-GDFWNWME 664
           A P+K  L +   K+L  A C+IVL +I FP + F + K  L +G   L + G+FW W+E
Sbjct: 663 AQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGGEFWTWLE 694

Query: 665 TLWAWNSATFLYLGMVHLPCWVLCTQILFH 692
           +LWAW SAT+LY+GMVHLPCW+LC  ILFH
Sbjct: 723 SLWAWKSATYLYIGMVHLPCWLLCLCILFH 694

BLAST of Spg039967 vs. TAIR 10
Match: AT5G19130.1 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 713.8 bits (1841), Expect = 1.4e-205
Identity = 375/690 (54.35%), Postives = 484/690 (70.14%), Query Frame = 0

Query: 5   FTHDNHVPQGSANTMLSGQEVSEANNLVKDLKGLNSKSGSTVLYVPTALFYFIYDYVVCA 64
           +  +N +  GSA +MLS ++VS+ + LVKD+K          + V               
Sbjct: 63  YISENALMPGSARSMLSNRDVSDGSKLVKDIKNFRLNHEGQGVEV--------------- 122

Query: 65  CGSACHFIGSQQIIAQYISNLGAEVNYHRFHPLFSQFHPLHFFSSPDSGIIRDNVSCMTQ 124
                     Q++I +Y+S++GAEV+Y +FHP  +QFHPLHFFS PDS  + +NVSC + 
Sbjct: 123 ----------QKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASY 182

Query: 125 GINTVGIIRAPQADGKEAIVLVTPYNPVRTSLHETLSLGIAYSVFSLLTQVTWLAKDIVW 184
           G+N  GIIRAP+ DGKE+IVLVTPY+ +    +E LSLGI  S+FSLL++VTWL+KDI+W
Sbjct: 183 GVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVSSLFSLLSRVTWLSKDIIW 242

Query: 185 LVADSRYGEYAAVSAWLRDYHTPAFGRSSIINTDACTESNVLYELEANHMTEKRSLDDFK 244
           LVADSRYG+Y  V+AWL +YH+P+F  S ++  D                 E+ + D+F+
Sbjct: 243 LVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD-----------------EQNTADNFR 302

Query: 245 RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEK 304
           RAGTMAAALV+KV  +SE FED+LS+YAEASNGQMPNLDLIN+VNYLAVHRQGF +K+EK
Sbjct: 303 RAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEK 362

Query: 305 FWPLLDCKWLKVLGEVFESIGKFIRSLNPEWKFGMPASDYVDGTATLASSLYYQAVGIPT 364
              LL   WLK+ GE+FE++GK    LNP+W FG+PA+DY++G+ATLASSLY QA+GIPT
Sbjct: 363 VVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPT 422

Query: 365 GPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFF 424
           GPHGAFRDYQ+DAIT+++SP+F    K R+ +F LRG RL+EG +RSVNNLLEKFHQSFF
Sbjct: 423 GPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFF 482

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKAEPAVPANADDVL 484
           LY++ S SKF+SVGVYMIAFALLVAPLP VAASLY      +LT     P          
Sbjct: 483 LYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLY-IDGCKSLTNSTHNPT--------- 542

Query: 485 IVSLRSWKWLNAAKRVFVVYLWGAVVSLLPYFICQIPG-YSPTTNTIIWALLSLLTLLIL 544
             + +SWKWL+AAK+VF ++L G +V+LLPYFICQ+PG +SPT  +I+W   S   LLI 
Sbjct: 543 -ENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLIT 602

Query: 545 SVILGSPLGSTKSSEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLL 604
            V +  P  S  SS      WA LK++T SAAFIGLCLMS+INFATAE+GA L+V  CL+
Sbjct: 603 FVTM--PGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLM 662

Query: 605 AHPLKLDLMAGSFKALSRAACNIVLGSIAFPPVTFFLFKAAL-QGFDNLHI-GDFWNWME 664
           A P+K  L +   K+L  A C+IVL +I FP + F + K  L +G   L + G+FW W+E
Sbjct: 663 AQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGGEFWTWLE 697

Query: 665 TLWAWNSATFLYLGMVHLPCWVLCTQILFH 692
           +LWAW SAT+LY+GMVHLPCW+LC  ILFH
Sbjct: 723 SLWAWKSATYLYIGMVHLPCWLLCLCILFH 697

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023550992.10.0e+0088.82glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 [Cucurb... [more]
XP_023550991.10.0e+0088.82glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
KAG7016207.10.0e+0087.95Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma... [more]
XP_038891215.10.0e+0088.24glycosylphosphatidylinositol anchor attachment 1 protein isoform X2 [Benincasa h... [more]
XP_038891214.10.0e+0088.24glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Benincasa h... [more]
Match NameE-valueIdentityDescription
Q9WTK31.5e-2324.57Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=1009... [more]
O432921.3e-2225.26Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606... [more]
Q9US482.6e-1522.46GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
P390123.2e-1326.07GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JZW50.0e+0088.24glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucu... [more]
A0A6J1K1W50.0e+0088.24glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1FHQ40.0e+0087.81glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1FMH60.0e+0087.81glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucu... [more]
A0A6J1H9W90.0e+0086.50glycosylphosphatidylinositol anchor attachment 1 protein-like OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
AT5G19130.28.1e-20654.35GPI transamidase component family protein / Gaa1-like family protein [more]
AT5G19130.11.4e-20554.35GPI transamidase component family protein / Gaa1-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007246GPI transamidase component Gaa1PIRSFPIRSF036762GAA1coord: 5..51
e-value: 11.0
score: 2.1
coord: 71..693
e-value: 1.8E-170
score: 566.4
IPR007246GPI transamidase component Gaa1PFAMPF04114Gaa1coord: 124..685
e-value: 1.1E-109
score: 367.8
IPR007246GPI transamidase component Gaa1PANTHERPTHR13304GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 9..691
NoneNo IPR availablePANTHERPTHR13304:SF0GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 9..691

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039967.1Spg039967.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0016021 integral component of membrane