Spg039898 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039898
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionvery-long-chain enoyl-CoA reductase-like
Locationscaffold10: 42787977 .. 42791971 (+)
RNA-Seq ExpressionSpg039898
SyntenySpg039898
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAGATATTTTCTCTATACTATTACTTATCATTTGCTCTTCTTATTTTTTTGGGAGTGATGACACAGACACCAATCCGTAGCCAAATTTTGCTGCAATTAAGAATTTAGGTTCCCGTTCATCCCAATAAATTTATTCAGCTGGTTGTCGGCCTCCAGCCTCACAGACAGAACGATGTGTCGAATTGTAATGAGATGATTAGGAAGACGGTTCATCGGTCATCGGAATTAGCTCTCCTACCCCAACTGTACCTCATTTAATAGCCTACTTCCCAACCTCCCCCTTCAAAACCCACTTCAAACCCAGAAAACTTTTTCCATTTTCTAATAAAATCCATTTGCGGATCTCATCATATAACCACTTCTTGATTCTGGGTCCTCTCATTCTGCTCTTTCAGGATCTGGGTTGAAATAATAGGGCTATTTGCCCAACTGGGGTTCTCTAATTTGGCTGCAAAATGAAGGTCTCTGTGGTTTCTCGCAGTGGGAGAGAGATTGTTAAGGGCGGCATTCAACTGAGCGATTCTGTAAGTGTTCGGATTTTGATTGAATTACTGTTTGTTTCTCACTTCTCCTTTCGATTTTTTGGAAAAGAAGGAAGGATGAGAAGAGTACAACTTGTTTTGGTGGATGTGTTCAGTTTATAGACCTGTCTATTTGGCTTCTTAGAGATGAGTAGAAGATGGGACTTTAAGTTTCATGGAAATTGTTGTTTTATTTTTTTGCAGGCTTCGGTGGCTGATCTGCAGGATGCGATTTATAAGCGAAGTGAGTGCAAGATAGTAGAATGTTTCCTTTAATTGTTGCATACATGCCCTTATTAGCTTAGATTTTTTCGATTGGTTAGCTTAGATTCGACATTTTTATAGGAGTGAGTGACAGAACTGGAGTTCGCGGATGTTATACTACCATATCTACTTGTGGTTTGACCCAGTTTGGTAGTTGTTATAAGGATGAATCTAAAAGATTAAGTTTAGCCTAGCCTGAAAGTTTAAGCTTTTTGAGTTCAACAACATATAGAGATGATGATTCAAACCTATTACCTTTCGGTTGAGGATATAATGCCTTAACAGTTCAAGCTCTTGGGTAAGGAATCGAATTTTTGTTAACTAGTTCAGAGATTGTAAATTATTTCAAAAAGTTCATTGTTTTCCTATCTCATGCTTCCAGAAAAGCAGTCTTTTTATTACTAGTGGTTAGAAGAGACAGGAATTGATCTTCGAGTATGCTGATTTCTTCTGTTTTTACCCCTTGACCATATGATTATCGTTCCACCTTTATTATGTTGCATAGAGTTGCAGGCAAAGAATGAAATGATTTCGTTAATAGCTGGTTAGTTTCTTTCTTTCCTGGATGGGGTGAGGAGTTAGTGTAAAATCAAATGTTGGGGTTCAATATGATTGCAAATGCACCTCAATAGCTTTAAGATAAATATTCATAGTATGGTTGTGGTGTAAAAACTAATGAAAAGAACCTTGCCTCTCAATTTTCTAACAAAATGCATACTTGCTAATTGGCATTCTAGATAGACAATCCCTCCCTGTCCTTAGCAAGGTTTTTCTGTCTGAATTTTTTACGAGAAAACCTCAATGTCAAAAAATTGAATGAGGGAGCTGTATAAATATATATTTTTTAGTTTTTGAAATTACTATGTAAATTTGTATTTCAAACAGCAAGGAGTTCATTAGAATAGCTCCTAAATCCTAATGCTTATCTTATTACCATGCTGGTATACTTTGTTAAAATAATTCTGCATTTGCATTAATGCCTTCAGGCTTTTGATATGCATTGTAGCTTGGCTGGAGAATTTTCTTATTAGAGAAGGAAAACTATGCTTTGCAGGCTTCATTTTGCAATATTTTATTGATATTTACTTTGAGCATCATTATCCTCGCGACATAGCGCCTTTCTTGTTGTCATCTATGATTAAACATAAAGTACCTGAACAAAATTTACTGTTGATAGAATCCTCCACTCCACTATAAGTTCTATCAAGAAGCAAAAACAAAAAGAAATCTTAAACGACTTATACTTGATAGTGCCTGTAGTTCTTATAAATGAAACGTTTGATATTTTTGCCTGATATTGTTACGTGGTTCTTAAGCAGTGGCAAATTTTCAGTCGTTTAGCATGCATTCATATAATTCGATATCAGTTTTTATGTAAAGTGCAGCTACCTGATTTCCTTCTTTCACTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCGTTCAACCTGGTTCAAAGGAGAGGCCTACTGTTCTGAACTACAAGAAAAGCCTCAGAGAGTATTGTAGTGACAACTCGGACAATATAACCATTGTCTTCAAGGACTTGGGCCCGCAAGTTTCTTATCGTACACTATTCTTTTTTGAATACTTGGGTCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTATATCAGTTCTTCGGCTACAATGCAGAACGTGTCATCCATCCGGTTCAGACATATGCGATGTACTACTGGTGCTTTCACTATTTTAAGCGAATCATGGAAACATTTTTCGTGCATAGGTTCAGCCACGCAACCTCACCACTTTCGAATGTGTTCCGGAACTGTGCTTACTACTGGTCCTTTGGCTCATATATTGCTTATTATGTCAATCACCCACTTTATACCCCTGTTGGTGATCTTCAAATGAAGATTGGCTTCGCATTTGGTTTGCTGTGTCAACTGTCAAACTTCTACTGTCACATCTTGCTGAGACAATTGCGCAGCCCTGAGGGTAATGGGGGATATCAAATCCCAAAGGGGTTTCTTTTCAACATTGTGACATGTGCAAATTACACAACAGAAATATATCAGTGGGTTGGTTTCAATATAGCTACACAGACTGTTGCTGGTTACATCTTTCTCGTGGTGGCAACTCTTATCATGACGAACTGGGCCCTCGCAAAGCACCGTCGCCTCAAAAAGGTAGCATCTTTTTTAGTGGTTTAAGCTTCTAGGTTCAGTGATAATTCAATGGTTTTTGATATTAGGTTAAATTACCTCTAAACTCAAAAGCTTTAAGCTGATAGGTTAAAGTCTATTTATCTTTTTCATATATGGTCAACACTCTACTTAGTTAGTGGACTTGGGAACTAACACGGAATCCGATAAAGGGATTATCAATGTTAATTAGAGAAGAGGTCGAAAACAATACCTCTTGCTTTGATACTATATTATATCACTACTAAATTCAAAAGCTCAAACCGATAAATTATTAGTAAATTTCTATATTGTTTATAGTTAGCTTTGCTATATTTTGGCATGCTCTATTTAAACGTGCGATTCCCTTGGACTATAACTTCGCTCTCAAGTCTTGGAAACTCGAGATTACCATCATATTTTTGGATTTAGCTAGAGGCTTTGGAAGGGGCTACAGTTTTCGGTCTTGCAATTTCATTAGTTTTAGAGATAAAAGATGCCAATTTGGTAGATTCCTTGCACTTTTGTTGTGATTTTGCCTCTCATTTTGACAATGTTTTCCCCATTTGCAGCTGTTTGATGGGAAGGAGGGAAGGCCAAAGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAGAAGGATTGAGCTGCCTCATCAGCTCCTTATTTATCTCCTCAGCATTCGTTCAAGTTGGTAGCTTTAAATTAGAAGTGTCCCCTTGAAAGACACGACACGACATCATTTTTTTCCAGTGCAATTTATGTTCTTTGTTGTTGTCTTAACAGTATGGGCAATGGTCCTTTTGGTTTGTTTTGCTTTGGCGTCTAGTGTACTGTTGGTGGCTGATGGCTGCATGTTTTCTTTTGATAGCCTTTTAAATTCTCTCTGCTTCACCTCCTTTCCTGTTAGTTTGAGGACGTTCTTTGAACTGCTTTTTTGTACTGATATATGTTAGATGGCTCTAAATTATTGTGTTCTGATTGAATTTTGGCACCACAAAGAAGAAAAAAAGTCGATATTGAAGTCGTCTAGATCATTC

mRNA sequence

ATGAAGGTCTCTGTGGTTTCTCGCAGTGGGAGAGAGATTGTTAAGGGCGGCATTCAACTGAGCGATTCTGCTTCGGTGGCTGATCTGCAGGATGCGATTTATAAGCGAAAGTTGCAGGCAAAGAATGAAATGATTTCGTTAATAGCTGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCGTTCAACCTGGTTCAAAGGAGAGGCCTACTGTTCTGAACTACAAGAAAAGCCTCAGAGAGTATTGTAGTGACAACTCGGACAATATAACCATTGTCTTCAAGGACTTGGGCCCGCAAGTTTCTTATCGTACACTATTCTTTTTTGAATACTTGGGTCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTATATCAGTTCTTCGGCTACAATGCAGAACGTGTCATCCATCCGGTTCAGACATATGCGATGTACTACTGGTGCTTTCACTATTTTAAGCGAATCATGGAAACATTTTTCGTGCATAGGTTCAGCCACGCAACCTCACCACTTTCGAATGTGTTCCGGAACTGTGCTTACTACTGGTCCTTTGGCTCATATATTGCTTATTATGTCAATCACCCACTTTATACCCCTGTTGGTGATCTTCAAATGAAGATTGGCTTCGCATTTGGTTTGCTGTGTCAACTGTCAAACTTCTACTGTCACATCTTGCTGAGACAATTGCGCAGCCCTGAGGGTAATGGGGGATATCAAATCCCAAAGGGGTTTCTTTTCAACATTGTGACATGTGCAAATTACACAACAGAAATATATCAGTGGGTTGGTTTCAATATAGCTACACAGACTGTTGCTGGTTACATCTTTCTCGTGGTGGCAACTCTTATCATGACGAACTGGGCCCTCGCAAAGCACCGTCGCCTCAAAAAGCTGTTTGATGGGAAGGAGGGAAGGCCAAAGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAG

Coding sequence (CDS)

ATGAAGGTCTCTGTGGTTTCTCGCAGTGGGAGAGAGATTGTTAAGGGCGGCATTCAACTGAGCGATTCTGCTTCGGTGGCTGATCTGCAGGATGCGATTTATAAGCGAAAGTTGCAGGCAAAGAATGAAATGATTTCGTTAATAGCTGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCGTTCAACCTGGTTCAAAGGAGAGGCCTACTGTTCTGAACTACAAGAAAAGCCTCAGAGAGTATTGTAGTGACAACTCGGACAATATAACCATTGTCTTCAAGGACTTGGGCCCGCAAGTTTCTTATCGTACACTATTCTTTTTTGAATACTTGGGTCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTATATCAGTTCTTCGGCTACAATGCAGAACGTGTCATCCATCCGGTTCAGACATATGCGATGTACTACTGGTGCTTTCACTATTTTAAGCGAATCATGGAAACATTTTTCGTGCATAGGTTCAGCCACGCAACCTCACCACTTTCGAATGTGTTCCGGAACTGTGCTTACTACTGGTCCTTTGGCTCATATATTGCTTATTATGTCAATCACCCACTTTATACCCCTGTTGGTGATCTTCAAATGAAGATTGGCTTCGCATTTGGTTTGCTGTGTCAACTGTCAAACTTCTACTGTCACATCTTGCTGAGACAATTGCGCAGCCCTGAGGGTAATGGGGGATATCAAATCCCAAAGGGGTTTCTTTTCAACATTGTGACATGTGCAAATTACACAACAGAAATATATCAGTGGGTTGGTTTCAATATAGCTACACAGACTGTTGCTGGTTACATCTTTCTCGTGGTGGCAACTCTTATCATGACGAACTGGGCCCTCGCAAAGCACCGTCGCCTCAAAAAGCTGTTTGATGGGAAGGAGGGAAGGCCAAAGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAG

Protein sequence

MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL
Homology
BLAST of Spg039898 vs. NCBI nr
Match: XP_038890322.1 (very-long-chain enoyl-CoA reductase [Benincasa hispida])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 299/323 (92.57%), Postives = 306/323 (94.74%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLP+QPGSKERPTVLNYKKSLREYCSDNSDNIT++FKDLGPQVSYRTLFF+EYLGPLILY
Sbjct: 61  TLPIQPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLGPQVSYRTLFFWEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQFFGY AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLR+LRSPEGNG
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRKLRSPEGNG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. NCBI nr
Match: XP_022938888.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita moschata] >KAG6578697.1 Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016235.1 Very-long-chain enoyl-CoA reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 612.8 bits (1579), Expect = 1.7e-171
Identity = 297/323 (91.95%), Postives = 307/323 (95.05%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLNYKKSLREYCSDNSDNI++VFKDLGPQVSYRTLFF+EYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNISVVFKDLGPQVSYRTLFFWEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQ FGYNAER+IHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPV DLQMKIGFAFGL+CQ+SNFYCHILLR+LRSPEG+G
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLVCQVSNFYCHILLRKLRSPEGSG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. NCBI nr
Match: XP_008458883.1 (PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis melo] >KAA0037664.1 very-long-chain enoyl-CoA reductase [Cucumis melo var. makuwa] >TYK22808.1 very-long-chain enoyl-CoA reductase [Cucumis melo var. makuwa])

HSP 1 Score: 610.9 bits (1574), Expect = 6.3e-171
Identity = 298/323 (92.26%), Postives = 305/323 (94.43%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQDAI+KR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLN KKSLREYCSDNSD+IT+VFKDLGPQVSYRTLFFFEYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQFFGY AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQFFGYQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQ+SNFYCHILLR+LRSPEGNG
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. NCBI nr
Match: XP_023550073.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.9 bits (1574), Expect = 6.3e-171
Identity = 297/323 (91.95%), Postives = 306/323 (94.74%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLNYKKSLREYCSDNSDNI++VFKDLGPQV YRTLFF+EYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNISVVFKDLGPQVLYRTLFFWEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQ FGYNAER+IHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPV DLQMKIGFAFGL+CQ+SNFYCHILLR+LRSPEG+G
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLVCQVSNFYCHILLRKLRSPEGSG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. NCBI nr
Match: XP_022921854.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita moschata])

HSP 1 Score: 610.9 bits (1574), Expect = 6.3e-171
Identity = 297/323 (91.95%), Postives = 305/323 (94.43%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+V GGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLNYKKSL+EYCS+NSDNIT+VFKDLGPQVSYRTLFFFEYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNYKKSLKEYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQFFGY AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQ+ NFYCHILLR+LRSPEGNG
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRP+YPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPRYPRRWVILPPFL 310

BLAST of Spg039898 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 7.5e-151
Identity = 248/323 (76.78%), Postives = 284/323 (87.93%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKV+VVSRSGRE++K  + L DSA+VADLQ+A +KR             AKKFYP+RQRL
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKR-------------AKKFYPSRQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPV PGSK++P VLN KKSL+EYC  N++++T+VFKDLG QVSYRTLFFFEYLGPL++Y
Sbjct: 61  TLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           P+FYYFPVY+F GY  + VIHPVQTYAMYYWCFHYFKRI+ETFFVHRFSHATSP+ NVFR
Sbjct: 121 PVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFG+YIAYYVNHPLYTPV DLQMKIGF FGL+CQ++NFYCHILL+ LR P G G
Sbjct: 181 NCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIP+GFLFNIVTCANYTTEIYQW+GFNIATQT+AGY+FL VA LIMTNWAL KH RL+
Sbjct: 241 GYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLR 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           K+FDGK+G+PKYPRRWVILPPFL
Sbjct: 301 KIFDGKDGKPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.4e-50
Identity = 119/322 (36.96%), Postives = 178/322 (55.28%), Query Frame = 0

Query: 3   VSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRLTL 62
           + VVS+  ++ V G    S S +V +L     K+++ +K  +             +R+ L
Sbjct: 4   IKVVSQRSKKEV-GSFSTSSSTTVGEL-----KKQISSKTRL-----------GTERIRL 63

Query: 63  PVQPGSKERPTVLNYKKSLREYCSDN-SDNITIVFKDLGPQVSYRTLFFFEYLGPLILYP 122
            V   + + P          +  S +   + T+ FKDLGPQ+S+  +F  EY GPL +YP
Sbjct: 64  AVPSKTSKLPNAFEALGKDSDLVSKHVGADSTLYFKDLGPQISWSLVFICEYAGPLFVYP 123

Query: 123 IFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRN 182
           IFY+      +G ++ +     Q  A+  +  HY KRI ET FVHRFSH T P+ N+F+N
Sbjct: 124 IFYFLS-NLIYGTDSPKSF--AQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKN 183

Query: 183 CAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNGG 242
           C+YYW   + ++Y+VNHPLYT     ++ +G    ++ ++ N+ CHI LR LR P G+  
Sbjct: 184 CSYYWGCTAMVSYFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGSTE 243

Query: 243 YQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKK 302
            +IP+G LF  V+C NYT EI  W+GF+I TQT+  +IF ++    M  WA+ KHRR +K
Sbjct: 244 RKIPRGLLFEFVSCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRK 299

Query: 303 LFDGKEGRPKYPRRWVILPPFL 324
            F       KYP+   IL PFL
Sbjct: 304 EFG-----DKYPKSRKILIPFL 299

BLAST of Spg039898 vs. ExPASy Swiss-Prot
Match: Q9N5Y2 (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=6239 GN=art-1 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 110/284 (38.73%), Postives = 154/284 (54.23%), Query Frame = 0

Query: 48  IAAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRT 107
           IA KK     +R  L V+P  K +P   + K S       +S    +  +DLGPQ++++T
Sbjct: 36  IAQKKLKLTEERQALRVEP--KGKPLADDQKLS---DLGLSSQKAVLYVRDLGPQIAWKT 95

Query: 108 LFFFEYLGPLILYPIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHR 167
           +F  EY GPL +YP+FY  P + +        +HP    A + W FHY KR+ ET F+HR
Sbjct: 96  VFMAEYAGPLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIHR 155

Query: 168 FSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDLQMKIGFAFGLLCQLSNFY 227
           F ++T P  N+ +NC+YYW F +++AY+VNHPL+TP   GDLQ+  G A  ++ +  N  
Sbjct: 156 FGNSTMPQFNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNLS 215

Query: 228 CHILLRQLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTVAGYI 287
            HILLR LR P G    +IPK        LFN V+C NYT E+  W+ F+I  Q++   I
Sbjct: 216 IHILLRNLR-PAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAII 275

Query: 288 FLVVATLIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 324
           F       M  WA  KHR   K F      P YP+    + PF+
Sbjct: 276 FTTAGFAQMAIWAQGKHRNYLKEF------PDYPKNRKAIVPFV 307

BLAST of Spg039898 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.5e-46
Identity = 115/278 (41.37%), Postives = 152/278 (54.68%), Query Frame = 0

Query: 52  KFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFF 111
           ++YPARQ L L  +  S +   VL                 T+ F+DLG Q+S+ T+F  
Sbjct: 42  QWYPARQSLRLDPKGKSLKDEDVLQKLPV--------GTTATLYFRDLGAQISWVTVFLT 101

Query: 112 EYLGPLILYPIFYY-FPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 171
           EY GPL +Y +FY+  P      Y+     H V   A     FHY KR++ET FVHRFSH
Sbjct: 102 EYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIKRLLETLFVHRFSH 161

Query: 172 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDLQMKIGFAFGLLCQLSNFYCHI 231
            T PL N+F+NC YYW F +++AYY+NHPLYTP   G  Q+K+  A  ++CQL NF  H+
Sbjct: 162 GTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHM 221

Query: 232 LLRQLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLV 291
            LR LR P G+   +IP        +LF +V+C NYT E+  W+GF I TQ +   +F +
Sbjct: 222 ALRDLR-PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSL 281

Query: 292 VATLIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILP 321
           V    MT WA  KHR   K F     R   P R  I+P
Sbjct: 282 VGFTQMTIWAKGKHRSYLKEF-----RDYPPLRMPIIP 305

BLAST of Spg039898 vs. ExPASy Swiss-Prot
Match: Q64232 (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.5e-46
Identity = 116/278 (41.73%), Postives = 152/278 (54.68%), Query Frame = 0

Query: 52  KFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFF 111
           ++YPARQ L L  +  S +   VL                 T+ F+DLG Q+S+ T+F  
Sbjct: 42  QWYPARQSLRLDPKGKSLKDEDVLQKLPV--------GTTATLYFRDLGAQISWVTVFLT 101

Query: 112 EYLGPLILYPIFYY-FPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 171
           EY GPL +Y +FY+  P      Y+     H V   A     FHY KR++ET FVHRFSH
Sbjct: 102 EYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVVHLACMCHSFHYIKRLLETLFVHRFSH 161

Query: 172 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDLQMKIGFAFGLLCQLSNFYCHI 231
            T PL N+F+NC YYW F +++AYY+NHPLYTP   G  Q+K+  A  ++CQL NF  H+
Sbjct: 162 GTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAIFVICQLGNFSIHM 221

Query: 232 LLRQLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLV 291
            LR LR P G+   +IP        +LF +V+C NYT E+  W+GF I TQ V   +F +
Sbjct: 222 ALRDLR-PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCVPVALFSL 281

Query: 292 VATLIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILP 321
           V    MT WA  KHR   K F     R   P R  I+P
Sbjct: 282 VGFTQMTIWAKGKHRSYLKEF-----RDYPPLRMPIIP 305

BLAST of Spg039898 vs. ExPASy TrEMBL
Match: A0A6J1FK64 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111444958 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.0e-172
Identity = 297/323 (91.95%), Postives = 307/323 (95.05%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLNYKKSLREYCSDNSDNI++VFKDLGPQVSYRTLFF+EYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNISVVFKDLGPQVSYRTLFFWEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQ FGYNAER+IHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPV DLQMKIGFAFGL+CQ+SNFYCHILLR+LRSPEG+G
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLVCQVSNFYCHILLRKLRSPEGSG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. ExPASy TrEMBL
Match: A0A5A7T2H3 (Very-long-chain enoyl-CoA reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00470 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 298/323 (92.26%), Postives = 305/323 (94.43%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQDAI+KR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLN KKSLREYCSDNSD+IT+VFKDLGPQVSYRTLFFFEYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQFFGY AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQFFGYQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQ+SNFYCHILLR+LRSPEGNG
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. ExPASy TrEMBL
Match: A0A6J1JQU0 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111489004 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 298/323 (92.26%), Postives = 306/323 (94.74%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLNYKKSLREYCSDNSD I++VFKDLGPQVSYRTLFF+EYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDIISVVFKDLGPQVSYRTLFFWEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQ FGYNAER+IHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQLFGYNAERIIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPV DLQMKIGFAFGLLCQ+SNFYCHILLR+LRSPEG+G
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVSDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGSG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. ExPASy TrEMBL
Match: A0A0A0KNT8 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517090 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 298/323 (92.26%), Postives = 305/323 (94.43%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+VKGGIQLSDSASVADLQDAI+KR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLN KKSLREYCSDNSD+IT+VFKDLGPQVSYRTLFFFEYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQFFGY AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQ+SNFYCHILLR+LRSPEGNG
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRPKYPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. ExPASy TrEMBL
Match: A0A6J1E1Q3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111429991 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 297/323 (91.95%), Postives = 305/323 (94.43%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKVSVVSRSGRE+V GGIQLSDSASVADLQDAIYKR              KKFYPARQRL
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYKR-------------TKKFYPARQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPVQPGSKERPTVLNYKKSL+EYCS+NSDNIT+VFKDLGPQVSYRTLFFFEYLGPLILY
Sbjct: 61  TLPVQPGSKERPTVLNYKKSLKEYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           PIFYYFPVYQFFGY AERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR
Sbjct: 121 PIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQ+ NFYCHILLR+LRSPEGNG
Sbjct: 181 NCAYYWSFGSYIAYYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK
Sbjct: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           KLFDGKEGRP+YPRRWVILPPFL
Sbjct: 301 KLFDGKEGRPRYPRRWVILPPFL 310

BLAST of Spg039898 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 5.3e-152
Identity = 248/323 (76.78%), Postives = 284/323 (87.93%), Query Frame = 0

Query: 1   MKVSVVSRSGREIVKGGIQLSDSASVADLQDAIYKRKLQAKNEMISLIAAKKFYPARQRL 60
           MKV+VVSRSGRE++K  + L DSA+VADLQ+A +KR             AKKFYP+RQRL
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKR-------------AKKFYPSRQRL 60

Query: 61  TLPVQPGSKERPTVLNYKKSLREYCSDNSDNITIVFKDLGPQVSYRTLFFFEYLGPLILY 120
           TLPV PGSK++P VLN KKSL+EYC  N++++T+VFKDLG QVSYRTLFFFEYLGPL++Y
Sbjct: 61  TLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIY 120

Query: 121 PIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFR 180
           P+FYYFPVY+F GY  + VIHPVQTYAMYYWCFHYFKRI+ETFFVHRFSHATSP+ NVFR
Sbjct: 121 PVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFR 180

Query: 181 NCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRQLRSPEGNG 240
           NCAYYWSFG+YIAYYVNHPLYTPV DLQMKIGF FGL+CQ++NFYCHILL+ LR P G G
Sbjct: 181 NCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAG 240

Query: 241 GYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLK 300
           GYQIP+GFLFNIVTCANYTTEIYQW+GFNIATQT+AGY+FL VA LIMTNWAL KH RL+
Sbjct: 241 GYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLR 300

Query: 301 KLFDGKEGRPKYPRRWVILPPFL 324
           K+FDGK+G+PKYPRRWVILPPFL
Sbjct: 301 KIFDGKDGKPKYPRRWVILPPFL 310

BLAST of Spg039898 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-11
Identity = 55/210 (26.19%), Postives = 96/210 (45.71%), Query Frame = 0

Query: 115 GPLILYPIFYYFPVYQFFGYNAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSP 174
           G L+LY   +      FF   ++ +   +   A+     H+FKR+ E  F+H++S   + 
Sbjct: 68  GMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSAL---ALHFFKRVFEVLFIHKYSGGMA- 127

Query: 175 LSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKI-GFAFGLLCQLSNFYCHILLRQL 234
           + +     + Y+S  + + Y  N  L        MK+ G    ++  + N Y H+LL +L
Sbjct: 128 IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKL 187

Query: 235 RSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWAL 294
           R  +G   Y+IPKG LF+I+ C +Y  EI  +  F + +QT+  + F +   L +   + 
Sbjct: 188 RKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSY 247

Query: 295 AKHRRLKKLFDGKEGRPKYPRRWVILPPFL 324
           A        FD       +P+    L PF+
Sbjct: 248 ATRTWYLSKFD------DFPKHIKALIPFV 267

BLAST of Spg039898 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.5e-05
Identity = 33/115 (28.70%), Postives = 56/115 (48.70%), Query Frame = 0

Query: 213 FAFGLLCQLSNFYCHI----LLRQLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGF 272
           F  G++  ++  Y +I     L +L+  E  GGY IP+G  F +V+C NY  E  +W+G+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLKK-ENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 273 NIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 324
            + T + AG  F +     +   A A H+     F     + +YP+    + PF+
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKF-----KEEYPKTRKAVIPFV 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890322.11.1e-17292.57very-long-chain enoyl-CoA reductase [Benincasa hispida][more]
XP_022938888.11.7e-17191.95very-long-chain enoyl-CoA reductase-like [Cucurbita moschata] >KAG6578697.1 Very... [more]
XP_008458883.16.3e-17192.26PREDICTED: very-long-chain enoyl-CoA reductase [Cucumis melo] >KAA0037664.1 very... [more]
XP_023550073.16.3e-17191.95very-long-chain enoyl-CoA reductase-like [Cucurbita pepo subsp. pepo][more]
XP_022921854.16.3e-17191.95very-long-chain enoyl-CoA reductase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M2U27.5e-15176.78Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ... [more]
Q55C174.4e-5036.96Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn... [more]
Q9N5Y21.6e-4738.73Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=... [more]
Q9NZ018.5e-4641.37Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1[more]
Q642328.5e-4641.73Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FK648.0e-17291.95very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A5A7T2H33.0e-17192.26Very-long-chain enoyl-CoA reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1JQU03.0e-17192.26very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A0A0KNT83.0e-17192.26S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G5170... [more]
A0A6J1E1Q33.0e-17191.95very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT3G55360.15.3e-15276.783-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT5G16010.11.4e-1126.193-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G38050.11.5e-0528.703-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 172..322
e-value: 1.6E-27
score: 96.3
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 212..293
score: 22.110218
IPR0393573-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductasePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 1..323
NoneNo IPR availablePANTHERPTHR10556:SF28SC2coord: 1..323
NoneNo IPR availableCDDcd01801Ubl_TECR_likecoord: 2..99
e-value: 5.83293E-17
score: 72.3191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039898.1Spg039898.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function GO:0016631 enoyl-[acyl-carrier-protein] reductase activity
molecular_function GO:0004318 enoyl-[acyl-carrier-protein] reductase (NADH) activity
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors