Spg039874 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039874
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionmRNA-decapping enzyme subunit 2-like
Locationscaffold10: 42021494 .. 42025634 (+)
RNA-Seq ExpressionSpg039874
SyntenySpg039874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGCTCACCACTCCTCTTCTACTGCTCCGGTCAAAAATGGCCTTCCTCCTCAGGAGCTCCTCGACGATCTTTGTAGGTCAGTCCTTCAACTCAACTCTCTCTCTCTTTCTCTCCAGTTTTGGCAGTTGTTCTTGTTCTTGAACTACTCGATAATGTGCTGCGAGCATCGCATTCTTTTCAGGTTTTGTTTATACGACTGATCGTTTGACTGAGTTCTTTTTTGTTTTAATGCCTTTCACATTCTATCGATTTCTGTTCTGCCGCGCGATTGTGAGCTTTTGTTCGATCGTTATTGCTAGAAATTGAAAAAGAAGTGTAGTACGTTTAGTTGCATGAAATTTTCCTTAAAAAACGGCGATGAAGTATAGGAAGTTGAATTTTGGAGTAGAAATGCGGTCTATGAGAAAGGAAAATAGGTGATTGGCTCCGTGAATTTCTCTATCTTTACTGACTTAGCTTAGACATGCTTGATGTTGAACAATCTCTGTTTATGATAGCGAGCATGGATAGTCCATAACTTTGTTAAGATGAGTTTGTTGGCACATCTTTGAATTGGCGGTTGGATTATTTTATGTTAGAAGCAACATTATGTTCTCCAATGTAATAAGAGGTGAAGTTATTGTGATGAAGAAAACTGATGAGTGATACTCTTGTTAGATGTCGGAGGATGGTTAATGTGAGGGAAAACGTCACGTAAGATGGGGGCATAGAATTTAAATCAGGCATTATGAGCATGAGGAAGGATTGTGCTGAGCTCTGTTTGCTTAATGTTGTGGTGATCTTAGTGTTAAAATGAAGTTTCCAGAACTGACACAGTAAGAAACCTTTCACGCAGTTTAGAATGGATAGATGGAGTTTCTTTTAGGTAAATAAGTTGTTACTTGAACAAAATATTGTTGTTTCCTAGACGCATTACATCGCTAGTTTTTGTATGATATGTGATCTTGATGGATCTTCAATTCATTCTCCGTTAGTTTCCTTATTTCCTAACCAGTTTGGTGCAGCTCTGAATCTCTTTTGCTCTTCCTTTTATTTGCTTCAATATGTTGGGTTGATTTTTTGCAGTCGGTTTGTTTTGAATGTGCCGAAGGAAGATCTACAGTCATTTGAAAGGATTCTATTCCTCATAGAGTATGCACATTGGTTTTATGAAGACAATTCGGTGGAAAGAAATCCATCTTTAAAGTCATTAAATCTGAAGGAGTTCACTTCATTACGTATCCTCCACAGTTGTGAAACCAATTGATTATGAGAAAATAATAGTGGGAGTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCAAATAATGATTAATACTGGTTAGTGGTATACTTTATTTTTCTCTTCTTAATCCAGATTAATGTGCCTATTGTTATCTGTGATTCTGCACATTCCTGTTGCTCCTTATTATAGATCAGTGTTTAAAAGCTGCGATGTTCTAAAACCATATGTCGCCCATCTTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATAATGGATGAGACCTTTGAACGGGTGAGCTCTTGTTAATGAAGCTCAGCACACTTGAAAATTGTTTAAGCTGTTTTTTACCGTATGATGTAAAGGTTGTCGACGCTGTTCCCTTTTGCTTCTTACTGGCAAAATACAGTGCTTGTTGGTGAAAGGGTGGAAAGGATCAAGTTGGAGTTTTCCACGTGGTAAAAAGAGCAAAGACGAGGAAGACCATGCTTGTGCCATTAGAGAAGTAATCTTGATTCTTTTCGATTTTTCTCATCGCATTTTCAGCATTCTCATGTCATGTAAATACTACTTGGATTGTAACTGCAAAATTCACTTGAAGTTTAATGAATTAGTTGTCAGATGTTATTCCATTTCCATCTGTAGGATCTGGCCAAGAAAGACTGAATCTTGGTTTGATGATTTATATATAGATGTTGAAAAATTCCTCTGTTATTGTCAACTTTTTCAGACATTGATCACCTGTTGACAGTTATTTAATCACTTGCTGCATCTCCTATAAATATGAGATCTCCTATGTGAATGCAGGTCCTGGAAGAAACAGGATTTGACATTACAAATCTTATCAACAAATCTGAATACATAGAGGTTATATTTGGACCACAAAGGGTGCGGCTTTACATCATTGCTGGTGTAAAAGATGATAATGTCTTTGCCCCACAAACTAAGAAAGAGATCAGTGTACGTGTTTTTCCTTACCTCAAAAATATGTTTCTTAGAGTTTTAGTTTTGATCTACATTGATATCACTACTTCAGAAATACCAATATTCTTCTCACCAAAAAAAAGATATCAATATTCTCGGTACTTGATAGAACTCTCTTTTCTCAATGCTACTTGATAGAACTCTCTTTTCTCAATGCTGTATTTTTTATGCCATATTGTACTCATGCAACCATGATGCTATCGAAGAAGAATGTGCTGATGAATCTTTACTAGTCTTTTTTTTTCCATGAACCTTTTTGTAACTCCATTTTCAGGCTCTCGGGTTTAAGCACACTCTTTTTTGGTACATCAATGAAATATTTCCTTAAAAAAATTTAATGGTTTTGGAAATTATGAATTATCATCTGATTCTAGATTATGCTCATGTGACCATGATGCAACAGAAGAAGGATGCGTGGATGAATCTTGAGTTTGGTCTATTCTTCTAGGCAGCATTGGCTTTCATCAGCTCTTCTTAGAGCTGTTCTGCAAGCATTTGGATCAGTAATCTATATCATATTTTAATTCTCAAGAATCTCATCTTTGTTTTTTAGTATTAAAATTTTCTGTAAGTTACTGCTCCATGCAATTTAAAATTTTTGGAGATTTAATAGCTTTAGGCGATGAGAAGATTTACCAACAGACTCTTGGTTATCCTGAAGCTTTTAAATTACCATGTCAAGCTTTTTGCGTTTAATAACATGTTCAACTCAAAGGGCTGTAATACTGATGAAAACTATAATATTGTTGGATTCAACTTAAGAGGTTTGGTTTCAAAATTCCTTTTGGGTGCCTGTCTGAAAAACTAAACATCTCCATGCCTATCACATTTTGAAAAAGTCGGTGGTAGCTTTCAATCTGTACTCAGAGATCCTAGGTTTCTCAAATTTCTGGGTATCTTAGGAAAAGGGGGAAACAGCAGAGATTCCAAATCCTAGGGTTTTTTTATGAAGATAGTATTGAAACTATTTCAGTGCTGTATGGATGGTTGTAACATCCTTCTTCTTTTCTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGATCACTTGTTTATTAGTGATGTGCCTTCAATGGAATCTTTTGTAGTTGAAAGCATGTACTTAACTATTTGTAAACAGGAAATTGCATGGCATCGACTTGATGATCTTGAACCAGCTGGTGATAATGTAATATCTCGTGGGATGAATGGTCTCAAGCTTTATATGGTTGCTCCTTTTTTGGCGTGAGTACACCTGTATATTCTTAATGATTTCTTCCACATCTTTAACGAATGTAGATATTTTAAGGGGTCAGAATCCTCATGCAGATCTCTAAGGTCATGGATTTCGACACATCAGCCCCCTGTAGCTCCAAACCCCGATGTGCCAATAAGAGGTTTCTTTTCTCCACTCATGGATTATAAGATTTTGCAGTTATTACCAATACGAGTTTGCTTCTGGTTTTCAAAGATAGATTCTTTTTTCCAAATCAAGTTATAGGCATCCAAATTTCAAGGCACAATCTAACGACTTTACATTCATTAAATCAAATAAACTTCCTTCCTAGTTTTTATTATACTAATCTGCCAATATGCCTTTCTGAACGAAGTTAATTTAATCTGTTCTTATTTTCAAGGACTAATCGTATGGAAGGCTAGGAACAGCTCGATAGGGAGCAGCAGTGCAATCGTAATGGAGAGCCAATTAAGTAAACCAGAAGTGGAGGCTTCCACCCCGGACACTGGTCCAGGCAGGAGCTTCAGAAACTTCAAGTTTGATACTGCCTCCATCTTACAGTCAATGGAAAGAGCCTTTTCCTAAGTCTTCTATAAGTCCTAAAGACCTTTTTTCTCAGTTGGTACATGTTTAGACCATATAGATATTTCACAAAGTTACTAACTAATAGTAATAGCC

mRNA sequence

ATGGCCGCCGCTCACCACTCCTCTTCTACTGCTCCGGTCAAAAATGGCCTTCCTCCTCAGGAGCTCCTCGACGATCTTTGTAGGTCAGTCCTTCAACTCAACTCTCTCTCTCTTTCTCTCCAGTTTTGGCAGTTGTTCTTGTTCTTGAACTACTCGATAATGTGCTGCGAGCATCGCATTCTTTTCAGTGTTAAAATGAAGTTTCCAGAACTGACACATCGGTTTGTTTTGAATGTGCCGAAGGAAGATCTACAGTCATTTGAAAGGATTCTATTCCTCATAGAATCAGTGTTTAAAAGCTGCGATGTTCTAAAACCATATGTCGCCCATCTTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATAATGGATGAGACCTTTGAACGGTGCTTGTTGGTGAAAGGGTGGAAAGGATCAAGTTGGAGTTTTCCACGTGGTAAAAAGAGCAAAGACGAGGAAGACCATGCTTGTGCCATTAGAGAAGTCCTGGAAGAAACAGGATTTGACATTACAAATCTTATCAACAAATCTGAATACATAGAGGTTATATTTGGACCACAAAGGGTGCGGCTTTACATCATTGCTGGTGTAAAAGATGATAATGTCTTTGCCCCACAAACTAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTTGATGATCTTGAACCAGCTGGTGATAATGTAATATCTCGTGGGATGAATGGTCTCAAGCTTTATATGGTTGCTCCTTTTTTGGCATCTCTAAGGTCATGGATTTCGACACATCAGCCCCCTGTAGCTCCAAACCCCGATGTGCCAATAAGAGGACTAATCGTATGGAAGGCTAGGAACAGCTCGATAGGGAGCAGCAGTGCAATCGTAATGGAGAGCCAATTAAGTAAACCAGAAGTGGAGGCTTCCACCCCGGACACTGGTCCAGGCAGGAGCTTCAGAAACTTCAAGTTTGATACTGCCTCCATCTTACAGTCAATGGAAAGAGCCTTTTCCTAA

Coding sequence (CDS)

ATGGCCGCCGCTCACCACTCCTCTTCTACTGCTCCGGTCAAAAATGGCCTTCCTCCTCAGGAGCTCCTCGACGATCTTTGTAGGTCAGTCCTTCAACTCAACTCTCTCTCTCTTTCTCTCCAGTTTTGGCAGTTGTTCTTGTTCTTGAACTACTCGATAATGTGCTGCGAGCATCGCATTCTTTTCAGTGTTAAAATGAAGTTTCCAGAACTGACACATCGGTTTGTTTTGAATGTGCCGAAGGAAGATCTACAGTCATTTGAAAGGATTCTATTCCTCATAGAATCAGTGTTTAAAAGCTGCGATGTTCTAAAACCATATGTCGCCCATCTTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATAATGGATGAGACCTTTGAACGGTGCTTGTTGGTGAAAGGGTGGAAAGGATCAAGTTGGAGTTTTCCACGTGGTAAAAAGAGCAAAGACGAGGAAGACCATGCTTGTGCCATTAGAGAAGTCCTGGAAGAAACAGGATTTGACATTACAAATCTTATCAACAAATCTGAATACATAGAGGTTATATTTGGACCACAAAGGGTGCGGCTTTACATCATTGCTGGTGTAAAAGATGATAATGTCTTTGCCCCACAAACTAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTTGATGATCTTGAACCAGCTGGTGATAATGTAATATCTCGTGGGATGAATGGTCTCAAGCTTTATATGGTTGCTCCTTTTTTGGCATCTCTAAGGTCATGGATTTCGACACATCAGCCCCCTGTAGCTCCAAACCCCGATGTGCCAATAAGAGGACTAATCGTATGGAAGGCTAGGAACAGCTCGATAGGGAGCAGCAGTGCAATCGTAATGGAGAGCCAATTAAGTAAACCAGAAGTGGAGGCTTCCACCCCGGACACTGGTCCAGGCAGGAGCTTCAGAAACTTCAAGTTTGATACTGCCTCCATCTTACAGTCAATGGAAAGAGCCTTTTCCTAA

Protein sequence

MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRILFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIESVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVFAPQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPNPDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEASTPDTGPGRSFRNFKFDTASILQSMERAFS
Homology
BLAST of Spg039874 vs. NCBI nr
Match: XP_022990407.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita maxima])

HSP 1 Score: 515.8 bits (1327), Expect = 2.9e-142
Identity = 273/369 (73.98%), Postives = 282/369 (76.42%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA H SSSTAPVKNGLPPQELLDDLC                                 
Sbjct: 1   MAALHSSSSTAPVKNGLPPQELLDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVLKPYVAH+DDIFKDFTSYKVRVPVTGAII+DET ERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRL DLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVA  
Sbjct: 241 APQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVASK 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKP--EVEASTPDTGPGRSFRNFKFDTASI 341
           PDV IRGL VWKA+NSSIGSSS IV+ESQLSKP  +V  STPDTGPG+SFRNFKFDTASI
Sbjct: 301 PDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASI 324

BLAST of Spg039874 vs. NCBI nr
Match: XP_038883995.1 (mRNA-decapping enzyme subunit 2-like [Benincasa hispida])

HSP 1 Score: 514.6 bits (1324), Expect = 6.4e-142
Identity = 271/367 (73.84%), Postives = 279/367 (76.02%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA HHSSSTAPVKN LP QELLDDLC                                 
Sbjct: 1   MAALHHSSSTAPVKNVLPSQELLDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVLKPYVAH+DDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDITNL+NKSEYIEVIFG QRVRLYIIAGVK DN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQRVRLYIIAGVKVDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRLDDLEPA DNVISRGMNGLKLYMVAPFLASLRSWISTH PPV PN
Sbjct: 241 APQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLASLRSWISTHHPPVHPN 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEASTPDTGPGRSFRNFKFDTASILQ 341
           PDV IRGL VWKA+NSSIGSSS I+MESQLSK EV+ S PDTGPGRSFRNFKFDTASILQ
Sbjct: 301 PDVSIRGLTVWKAKNSSIGSSSTIIMESQLSKQEVDVSIPDTGPGRSFRNFKFDTASILQ 322

BLAST of Spg039874 vs. NCBI nr
Match: XP_022964616.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >XP_023540752.1 mRNA-decapping enzyme subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.8 bits (1322), Expect = 1.1e-141
Identity = 272/369 (73.71%), Postives = 281/369 (76.15%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA H SS TAPVKNGLPPQELLDDLC                                 
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVLKPYVAH+DDIFKDFTSYKVRVPVTGAII+DET ERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRL DLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVA  
Sbjct: 241 APQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVASK 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKP--EVEASTPDTGPGRSFRNFKFDTASI 341
           PDV IRGL VWKA+NSSIGSSS IV+ESQLSKP  +V  STPDTGPG+SFRNFKFDTASI
Sbjct: 301 PDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASI 324

BLAST of Spg039874 vs. NCBI nr
Match: XP_004149909.2 (mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN47822.1 hypothetical protein Csa_003258 [Cucumis sativus])

HSP 1 Score: 508.1 bits (1307), Expect = 6.0e-140
Identity = 264/367 (71.93%), Postives = 279/367 (76.02%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA HHSSST PVKNGLPPQEL+DDLC                                 
Sbjct: 52  MAALHHSSSTTPVKNGLPPQELIDDLC--------------------------------- 111

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 112 ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 171

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVL+PYVAH+DDIFKDFTSYKVRVPVTGAII+DETFERCLLVKGWKGSSWSF
Sbjct: 172 TSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKGSSWSF 231

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDI+NL+ KSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 232 PRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLF 291

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRLDDLEPA DNVISRGM+GLKLYM+APFLASLRSWI TH PPV PN
Sbjct: 292 APQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHHPPVRPN 351

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEASTPDTGPGRSFRNFKFDTASILQ 341
           PD+PIRGL VWKA+NSSIGSSS I+MESQ+SK EVE    DTGPG+SFRNFKFDTASILQ
Sbjct: 352 PDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV---DTGPGKSFRNFKFDTASILQ 370

BLAST of Spg039874 vs. NCBI nr
Match: KAG6602315.1 (mRNA-decapping enzyme subunit 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032998.1 mRNA-decapping enzyme subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.5 bits (1303), Expect = 1.8e-139
Identity = 270/369 (73.17%), Postives = 279/369 (75.61%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA H SS TAPVKNGLPPQELLDDLC                                 
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVLKPYVAH+DDIFKDFTSYKVRVPVTGAII+DET ERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEED ACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDDACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRL DLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVA  
Sbjct: 241 APQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVASK 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKP--EVEASTPDTGPGRSFRNFKFDTASI 341
            DV IRGL VWKA+NSSIGSSS IV+ESQLSKP  +V  STPDTGPG+SFRNFKFDTASI
Sbjct: 301 LDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASI 324

BLAST of Spg039874 vs. ExPASy Swiss-Prot
Match: Q8GW31 (mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.5e-96
Identity = 200/413 (48.43%), Postives = 237/413 (57.38%), Query Frame = 0

Query: 1   MAAAHHSSSTAP-VKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHR 60
           M+  H SSS++  + N LP +ELLDDLC                                
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLC-------------------------------- 60

Query: 61  ILFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------ 120
                         RFVLNVP+ED QSFERILFL+E                        
Sbjct: 61  -------------SRFVLNVPEEDQQSFERILFLVEYAYWYYEDNAVENDPKLKSLSLKE 120

Query: 121 ---SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWS 180
               +F SCDVL+PYV H+DDIFKDFTSYK RVPVTGAII+DET+ERCLLVKGWKGSSWS
Sbjct: 121 FTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETYERCLLVKGWKGSSWS 180

Query: 181 FPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNV 240
           FPRGKKSKDEEDHACAIREVLEETGFD++ L+ + EYIE +F  QRVRLYI+AGV +D V
Sbjct: 181 FPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQRVRLYIVAGVTEDTV 240

Query: 241 FAPQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAP 300
           FAP TKKEISEI WHRLD L+PA + VI+ G++GLKLYMVAPFL+SL+SWI  H  PVA 
Sbjct: 241 FAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLSSLKSWILKHPSPVAR 300

Query: 301 NPDVPIRGLIVWKARNSSIGSSSAIV---------------------------MESQLSK 337
            P+ P++ L VW AR S  G+ +A V                           MES  +K
Sbjct: 301 RPNKPLKALCVWNARTSVGGNGTATVESQNRKSELRTTTMESNSRKPELKRTTMESHSTK 360

BLAST of Spg039874 vs. ExPASy Swiss-Prot
Match: O13828 (mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dcp2 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 105/277 (37.91%), Postives = 147/277 (53.07%), Query Frame = 0

Query: 70  ELTHRFVLNVPKEDLQSFERILFLIE---------------------------SVFKSCD 129
           +L+ RF+LN+P E+  S ER+ F IE                            +F  C 
Sbjct: 14  DLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLFAHCP 73

Query: 130 VLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSS-WSFPRGKKSKD 189
           +L  +    ++ F DF  YK R+PV GAI++D + ++C+LVKGWK SS W FP+GK  KD
Sbjct: 74  LLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGKIDKD 133

Query: 190 EEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVFAPQTKKEI 249
           E D  CAIREV EETGFD ++ IN +E+I++    Q VRLYII G+  D  F  +T+KEI
Sbjct: 134 ESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRTRKEI 193

Query: 250 SEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWI--------STHQPPVAPN 309
           S+I WH L DL     N      N  K YMV PFLA L+ WI        +T +  ++ +
Sbjct: 194 SKIEWHNLMDLPTFKKNKPQTMKN--KFYMVIPFLAPLKKWIKKRNIANNTTKEKNISVD 253

Query: 310 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEA 311
            D      ++   ++S+  S  A    S   +P VE+
Sbjct: 254 VDADASSQLLSLLKSSTAPSDLATPQPSTFPQPPVES 288

BLAST of Spg039874 vs. ExPASy Swiss-Prot
Match: Q9CYC6 (m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 6.5e-36
Identity = 85/226 (37.61%), Postives = 119/226 (52.65%), Query Frame = 0

Query: 70  ELTHRFVLNVPKEDLQSFERILFLIE---------------------------SVFKSCD 129
           +L  RF+L++P E+  +  R+ F IE                           +VF  C 
Sbjct: 15  DLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVFSHCP 74

Query: 130 VLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKG-SSWSFPRGKKSKD 189
            L P    ++ I  ++  YK+ VP  GAII+DET E  LLV+G+   S W FP+GK +K+
Sbjct: 75  FLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKE 134

Query: 190 EEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVFAPQTKKEI 249
           E  H CA REV EETGFDI + I K +YIE+    Q  RLYII GV  D  F P+T++EI
Sbjct: 135 EAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNPKTRREI 194

Query: 250 SEIAWHRLDDLEPAGDNVISRGMNGL---KLYMVAPFLASLRSWIS 265
             I W  ++ L    +++  +   GL   K +M  PF+  LR W+S
Sbjct: 195 RNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLS 240

BLAST of Spg039874 vs. ExPASy Swiss-Prot
Match: Q8IU60 (m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 83/226 (36.73%), Postives = 119/226 (52.65%), Query Frame = 0

Query: 70  ELTHRFVLNVPKEDLQSFERILFLIE---------------------------SVFKSCD 129
           +L  RF+L++P E+  +  R+ F IE                           +VF  C 
Sbjct: 15  DLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVFSHCP 74

Query: 130 VLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKG-SSWSFPRGKKSKD 189
            L P    ++ +  ++  YK+ VP  GAII+DET E  LLV+G+   S W FP+GK +K+
Sbjct: 75  FLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKE 134

Query: 190 EEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVFAPQTKKEI 249
           E  H CA REV EETGFDI + I K +YIE+    Q  RLYII G+  D  F P+T++EI
Sbjct: 135 EAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREI 194

Query: 250 SEIAWHRLDDLEPAGDNVISRGMNGL---KLYMVAPFLASLRSWIS 265
             I W  ++ L    +++  +   GL   K +M  PF+  LR W+S
Sbjct: 195 RNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLS 240

BLAST of Spg039874 vs. ExPASy Swiss-Prot
Match: Q5REQ8 (m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-35
Identity = 83/226 (36.73%), Postives = 119/226 (52.65%), Query Frame = 0

Query: 70  ELTHRFVLNVPKEDLQSFERILFLIE---------------------------SVFKSCD 129
           +L  RF+L++P E+  +  R+ F IE                           +VF  C 
Sbjct: 15  DLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLSQCGIRDFAKAVFSHCP 74

Query: 130 VLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKG-SSWSFPRGKKSKD 189
            L P    ++ +  ++  YK+ VP  GAII+DET E  LLV+G+   S W FP+GK +K+
Sbjct: 75  FLLPEGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKE 134

Query: 190 EEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVFAPQTKKEI 249
           E  H CA REV EETGFDI + I K +YIE+    Q  RLYII G+  D  F P+T++EI
Sbjct: 135 EAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREI 194

Query: 250 SEIAWHRLDDLEPAGDNVISRGMNGL---KLYMVAPFLASLRSWIS 265
             I W  ++ L    +++  +   GL   K +M  PF+  LR W+S
Sbjct: 195 RNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLS 240

BLAST of Spg039874 vs. ExPASy TrEMBL
Match: A0A6J1JIL9 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487274 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.4e-142
Identity = 273/369 (73.98%), Postives = 282/369 (76.42%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA H SSSTAPVKNGLPPQELLDDLC                                 
Sbjct: 1   MAALHSSSSTAPVKNGLPPQELLDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVLKPYVAH+DDIFKDFTSYKVRVPVTGAII+DET ERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRL DLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVA  
Sbjct: 241 APQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVASK 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKP--EVEASTPDTGPGRSFRNFKFDTASI 341
           PDV IRGL VWKA+NSSIGSSS IV+ESQLSKP  +V  STPDTGPG+SFRNFKFDTASI
Sbjct: 301 PDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASI 324

BLAST of Spg039874 vs. ExPASy TrEMBL
Match: A0A6J1HJG1 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464573 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.3e-142
Identity = 272/369 (73.71%), Postives = 281/369 (76.15%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA H SS TAPVKNGLPPQELLDDLC                                 
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVLKPYVAH+DDIFKDFTSYKVRVPVTGAII+DET ERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRL DLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVA  
Sbjct: 241 APQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVASK 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKP--EVEASTPDTGPGRSFRNFKFDTASI 341
           PDV IRGL VWKA+NSSIGSSS IV+ESQLSKP  +V  STPDTGPG+SFRNFKFDTASI
Sbjct: 301 PDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASI 324

BLAST of Spg039874 vs. ExPASy TrEMBL
Match: A0A0A0KHG2 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G405350 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.9e-140
Identity = 264/367 (71.93%), Postives = 279/367 (76.02%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA HHSSST PVKNGLPPQEL+DDLC                                 
Sbjct: 52  MAALHHSSSTTPVKNGLPPQELIDDLC--------------------------------- 111

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 112 ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 171

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVL+PYVAH+DDIFKDFTSYKVRVPVTGAII+DETFERCLLVKGWKGSSWSF
Sbjct: 172 TSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKGSSWSF 231

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDI+NL+ KSEYIEVIFGPQRVRLYIIAGVKDDN+F
Sbjct: 232 PRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLF 291

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRLDDLEPA DNVISRGM+GLKLYM+APFLASLRSWI TH PPV PN
Sbjct: 292 APQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHHPPVRPN 351

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEASTPDTGPGRSFRNFKFDTASILQ 341
           PD+PIRGL VWKA+NSSIGSSS I+MESQ+SK EVE    DTGPG+SFRNFKFDTASILQ
Sbjct: 352 PDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV---DTGPGKSFRNFKFDTASILQ 370

BLAST of Spg039874 vs. ExPASy TrEMBL
Match: A0A5D3BM04 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00650 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.8e-137
Identity = 260/366 (71.04%), Postives = 275/366 (75.14%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA  HSSST PVKNGLPPQEL+DDLC                                 
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVL+PYVAH+DDIFKDFTSYKVRVPVTGAI++DETFERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDI+NL+NKSEYIEVIFG QRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRLDDLEPA DNVISRGMNGLKLYMVAPFLASLRSWI TH PPV  N
Sbjct: 241 APQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLASLRSWILTHHPPVHSN 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEASTPDTGPGRSFRNFKFDTASILQ 340
           PD+PIRGL VWKA+NSSI SSS I+MESQ+SK EV+    DTGPG+SFRNFKFDTASILQ
Sbjct: 301 PDIPIRGLTVWKAKNSSISSSSTIIMESQISKAEVDV---DTGPGKSFRNFKFDTASILQ 318

BLAST of Spg039874 vs. ExPASy TrEMBL
Match: A0A1S3C3F4 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103496421 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.8e-137
Identity = 260/366 (71.04%), Postives = 275/366 (75.14%), Query Frame = 0

Query: 1   MAAAHHSSSTAPVKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHRI 60
           MAA  HSSST PVKNGLPPQEL+DDLC                                 
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLC--------------------------------- 60

Query: 61  LFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------- 120
                        RFVLNVPKEDLQSFERILFLIE                         
Sbjct: 61  ------------SRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEF 120

Query: 121 --SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSF 180
              +FKSCDVL+PYVAH+DDIFKDFTSYKVRVPVTGAI++DETFERCLLVKGWKGSSWSF
Sbjct: 121 TSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFERCLLVKGWKGSSWSF 180

Query: 181 PRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNVF 240
           PRGKKSKDEEDHACAIREVLEETGFDI+NL+NKSEYIEVIFG QRVRLYIIAGVKDDN+F
Sbjct: 181 PRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNIF 240

Query: 241 APQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAPN 300
           APQTKKEISEIAWHRLDDLEPA DNVISRGMNGLKLYMVAPFLASLRSWI TH PPV  N
Sbjct: 241 APQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLASLRSWILTHHPPVHSN 300

Query: 301 PDVPIRGLIVWKARNSSIGSSSAIVMESQLSKPEVEASTPDTGPGRSFRNFKFDTASILQ 340
           PD+PIRGL VWKA+NSSI SSS I+MESQ+SK EV+    DTGPG+SFRNFKFDTASILQ
Sbjct: 301 PDIPIRGLTVWKAKNSSISSSSTIIMESQISKAEVDV---DTGPGKSFRNFKFDTASILQ 318

BLAST of Spg039874 vs. TAIR 10
Match: AT5G13570.1 (decapping 2 )

HSP 1 Score: 351.7 bits (901), Expect = 6.7e-97
Identity = 200/413 (48.43%), Postives = 237/413 (57.38%), Query Frame = 0

Query: 1   MAAAHHSSSTAP-VKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHR 60
           M+  H SSS++  + N LP +ELLDDLC                                
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLC-------------------------------- 60

Query: 61  ILFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------ 120
                         RFVLNVP+ED QSFERILFL+E                        
Sbjct: 61  -------------SRFVLNVPEEDQQSFERILFLVEYAYWYYEDNAVENDPKLKSLSLKE 120

Query: 121 ---SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWS 180
               +F SCDVL+PYV H+DDIFKDFTSYK RVPVTGAII+DET+ERCLLVKGWKGSSWS
Sbjct: 121 FTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETYERCLLVKGWKGSSWS 180

Query: 181 FPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQRVRLYIIAGVKDDNV 240
           FPRGKKSKDEEDHACAIREVLEETGFD++ L+ + EYIE +F  QRVRLYI+AGV +D V
Sbjct: 181 FPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQRVRLYIVAGVTEDTV 240

Query: 241 FAPQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASLRSWISTHQPPVAP 300
           FAP TKKEISEI WHRLD L+PA + VI+ G++GLKLYMVAPFL+SL+SWI  H  PVA 
Sbjct: 241 FAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLSSLKSWILKHPSPVAR 300

Query: 301 NPDVPIRGLIVWKARNSSIGSSSAIV---------------------------MESQLSK 337
            P+ P++ L VW AR S  G+ +A V                           MES  +K
Sbjct: 301 RPNKPLKALCVWNARTSVGGNGTATVESQNRKSELRTTTMESNSRKPELKRTTMESHSTK 360

BLAST of Spg039874 vs. TAIR 10
Match: AT5G13570.2 (decapping 2 )

HSP 1 Score: 342.4 bits (877), Expect = 4.1e-94
Identity = 200/426 (46.95%), Postives = 237/426 (55.63%), Query Frame = 0

Query: 1   MAAAHHSSSTAP-VKNGLPPQELLDDLCRSVLQLNSLSLSLQFWQLFLFLNYSIMCCEHR 60
           M+  H SSS++  + N LP +ELLDDLC                                
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLC-------------------------------- 60

Query: 61  ILFSVKMKFPELTHRFVLNVPKEDLQSFERILFLIE------------------------ 120
                         RFVLNVP+ED QSFERILFL+E                        
Sbjct: 61  -------------SRFVLNVPEEDQQSFERILFLVEYAYWYYEDNAVENDPKLKSLSLKE 120

Query: 121 ---SVFKSCDVLKPYVAHLDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWS 180
               +F SCDVL+PYV H+DDIFKDFTSYK RVPVTGAII+DET+ERCLLVKGWKGSSWS
Sbjct: 121 FTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETYERCLLVKGWKGSSWS 180

Query: 181 FPRGKKSKDEEDHACAIRE-------------VLEETGFDITNLINKSEYIEVIFGPQRV 240
           FPRGKKSKDEEDHACAIRE             VLEETGFD++ L+ + EYIE +F  QRV
Sbjct: 181 FPRGKKSKDEEDHACAIRELSSAILLVNVAFQVLEETGFDVSKLLKREEYIEFVFRQQRV 240

Query: 241 RLYIIAGVKDDNVFAPQTKKEISEIAWHRLDDLEPAGDNVISRGMNGLKLYMVAPFLASL 300
           RLYI+AGV +D VFAP TKKEISEI WHRLD L+PA + VI+ G++GLKLYMVAPFL+SL
Sbjct: 241 RLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLSSL 300

Query: 301 RSWISTHQPPVAPNPDVPIRGLIVWKARNSSIGSSSAIV--------------------- 337
           +SWI  H  PVA  P+ P++ L VW AR S  G+ +A V                     
Sbjct: 301 KSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQNRKSELRTTTMESNSRKP 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022990407.12.9e-14273.98mRNA-decapping enzyme subunit 2-like [Cucurbita maxima][more]
XP_038883995.16.4e-14273.84mRNA-decapping enzyme subunit 2-like [Benincasa hispida][more]
XP_022964616.11.1e-14173.71mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >XP_023540752.1 mRNA-d... [more]
XP_004149909.26.0e-14071.93mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN47822.1 hypothetical prote... [more]
KAG6602315.11.8e-13973.17mRNA-decapping enzyme subunit 2, partial [Cucurbita argyrosperma subsp. sororia]... [more]
Match NameE-valueIdentityDescription
Q8GW319.5e-9648.43mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=... [more]
O138281.1e-4337.91mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9CYC66.5e-3637.61m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2[more]
Q8IU601.1e-3536.73m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3[more]
Q5REQ81.4e-3536.73m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JIL91.4e-14273.98mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487274... [more]
A0A6J1HJG15.3e-14273.71mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114645... [more]
A0A0A0KHG22.9e-14071.93Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G40... [more]
A0A5D3BM041.8e-13771.04mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C3F41.8e-13771.04mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103496421 PE=... [more]
Match NameE-valueIdentityDescription
AT5G13570.16.7e-9748.43decapping 2 [more]
AT5G13570.24.1e-9446.95decapping 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007722mRNA decapping protein 2, Box A domainSMARTSM01125DCP2_2coord: 65..122
e-value: 8.9E-6
score: 32.9
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 124..265
e-value: 3.3E-61
score: 208.4
NoneNo IPR availablePANTHERPTHR23114M7GPPPN-MRNA HYDROLASEcoord: 11..280
IPR036189mRNA decapping protein 2, Box A domain superfamilyGENE3D1.10.10.1050Dcp2, box A domaincoord: 95..123
e-value: 3.3E-61
score: 208.4
IPR036189mRNA decapping protein 2, Box A domain superfamilySUPERFAMILY140586Dcp2 domain-likecoord: 69..122
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 125..235
e-value: 1.4E-14
score: 54.2
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 123..252
score: 12.874132
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 156..177
IPR044099mRNA decapping enzyme 2 , NUDIX hydrolase domainCDDcd03672Dcp2pcoord: 125..267
e-value: 4.47076E-75
score: 225.965
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 125..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039874.1Spg039874.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050072 m7G(5')pppN diphosphatase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0016787 hydrolase activity