Spg039625 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039625
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionHeat shock 70 kDa protein
Locationscaffold10: 42695540 .. 42696967 (-)
RNA-Seq ExpressionSpg039625
SyntenySpg039625
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGATCATCAATGAGCCTACAGCCGCAGCTATCGCTTATGGACTCGACAAAAAGGCAAGCAGTGTCGGCGAGAAGAATGTACTGATATTTGATCTGGGTGGAGGAACATTTGATGTCTCTCTCCTCACAATTGAGGAAGGTATTTTTGAGGTGAAGGCAACAGCCGGTGACACCCATCTCGGAGGCGAGGACTTCGACAACAGATTGGTTAACCATTTCGTTCAGGAATTCAAGAGAAAGCACAAGAAGGACATCAGTGGAAATCCGAGAGCTCTTAGGAGATTGAGGACTGCTTGTGAGAGGGCCAAGAGGACCCTTTCATCCACTGCCCAAACTACAATCGAGATTGATTCTTTGTATGAGGGAATTGATTTCTATACAACCATCACTCGTGCTAGATTCGAGGAGCTCAACATGGACTTGTTCCGAAAGTGTATGGAGCCGGTTGAGAAGTGTCTGAGAGATGCAAAGATGGACAAAAGCACCGTCCATGATGTTGTCCTTGTTGGTGGCTCTACAAGGATTCCTAAGGTGCAGCAGCTATTGCAGGATTTCTTCAATGGCAAAGAACTCTGCAAAAGTATCAATCCTGATGAGGCAGTTGCTTATGGGGCTGCTGTCCAAGCGGCGATCTTGAGTGGTGAAGGAAATGAGAAAGTGCAGGATCTTCTTCTTTTGGATGTCACTCCTCTGTCTCTTGGTTTGGAAACTGCAGGAGGTGTGATGACAGTTTTGATTCCAAGGAATACCACCATACCCACCAAGAAGGAGCAAGTATTTTCCACATACTCAGACAACCAACCTGGTGTTCTCATTCAGGTCTACGAGGGTGAGAGGACAAGAACCAGAGATAATAACTTGTTGGGCAAGTTTGAACTTTCCGGCATCCCTCCGGCTCCGAGAGGTGTTCCACAGATCACTGTCTGCTTTGACATTGACGCCAACGGTATTCTAAACGTTTCTGCAGAGGACAAGACCACCGGACAGAAGAATAAGATCACCATTACCAACGACAAAGGAAGGTTGTCCAAAGAGGAGATCGAGAAACTGGTTCAGGAAGCTGAGAAATTCAAGGCCGAAGATGAGGAACACAAGAAGAAAGTTGAATCAAAGAACGCGTTAGAAAACTATGCATACAACATGAGGAACACTATCAGAGATGAAAAGATAAGTGGTAAACTTGCGCCAGCAGATAAGAAGAAGATTGAAGATGCAGTCGAACAGGCCGTCCAGTGGCTAGACAGCAACCAGCTGGCTGAGTCGGATGAATTTGAAGACAAGATGAAGGAACTTGAATCTATATGCAATCCCATCATAGCCAAGATGTATCAAGGTGGTGCTGACATGGGCGGCAGCGCAATGGATGATGATGCTCCTCCTTCTGGCGGCAGCGGCGCTGGTCCTAAGATTGAAGAAGTTGATTAA

mRNA sequence

ATGCGGATCATCAATGAGCCTACAGCCGCAGCTATCGCTTATGGACTCGACAAAAAGGCAAGCAGTGTCGGCGAGAAGAATGTACTGATATTTGATCTGGGTGGAGGAACATTTGATGTCTCTCTCCTCACAATTGAGGAAGGTATTTTTGAGGTGAAGGCAACAGCCGGTGACACCCATCTCGGAGGCGAGGACTTCGACAACAGATTGGTTAACCATTTCGTTCAGGAATTCAAGAGAAAGCACAAGAAGGACATCAGTGGAAATCCGAGAGCTCTTAGGAGATTGAGGACTGCTTGTGAGAGGGCCAAGAGGACCCTTTCATCCACTGCCCAAACTACAATCGAGATTGATTCTTTGTATGAGGGAATTGATTTCTATACAACCATCACTCGTGCTAGATTCGAGGAGCTCAACATGGACTTGTTCCGAAAGTGTATGGAGCCGGTTGAGAAGTGTCTGAGAGATGCAAAGATGGACAAAAGCACCGTCCATGATGTTGTCCTTGTTGGTGGCTCTACAAGGATTCCTAAGGTGCAGCAGCTATTGCAGGATTTCTTCAATGGCAAAGAACTCTGCAAAAGTATCAATCCTGATGAGGCAGTTGCTTATGGGGCTGCTGTCCAAGCGGCGATCTTGAGTGGTGAAGGAAATGAGAAAGTGCAGGATCTTCTTCTTTTGGATGTCACTCCTCTGTCTCTTGGTTTGGAAACTGCAGGAGGTGTGATGACAGTTTTGATTCCAAGGAATACCACCATACCCACCAAGAAGGAGCAAGTATTTTCCACATACTCAGACAACCAACCTGGTGTTCTCATTCAGGTCTACGAGGGTGAGAGGACAAGAACCAGAGATAATAACTTGTTGGGCAAGTTTGAACTTTCCGGCATCCCTCCGGCTCCGAGAGGTGTTCCACAGATCACTGTCTGCTTTGACATTGACGCCAACGGTATTCTAAACGTTTCTGCAGAGGACAAGACCACCGGACAGAAGAATAAGATCACCATTACCAACGACAAAGGAAGGTTGTCCAAAGAGGAGATCGAGAAACTGGTTCAGGAAGCTGAGAAATTCAAGGCCGAAGATGAGGAACACAAGAAGAAAGTTGAATCAAAGAACGCGTTAGAAAACTATGCATACAACATGAGGAACACTATCAGAGATGAAAAGATAAGTGGTAAACTTGCGCCAGCAGATAAGAAGAAGATTGAAGATGCAGTCGAACAGGCCGTCCAGTGGCTAGACAGCAACCAGCTGGCTGAGTCGGATGAATTTGAAGACAAGATGAAGGAACTTGAATCTATATGCAATCCCATCATAGCCAAGATGTATCAAGGTGGTGCTGACATGGGCGGCAGCGCAATGGATGATGATGCTCCTCCTTCTGGCGGCAGCGGCGCTGGTCCTAAGATTGAAGAAGTTGATTAA

Coding sequence (CDS)

ATGCGGATCATCAATGAGCCTACAGCCGCAGCTATCGCTTATGGACTCGACAAAAAGGCAAGCAGTGTCGGCGAGAAGAATGTACTGATATTTGATCTGGGTGGAGGAACATTTGATGTCTCTCTCCTCACAATTGAGGAAGGTATTTTTGAGGTGAAGGCAACAGCCGGTGACACCCATCTCGGAGGCGAGGACTTCGACAACAGATTGGTTAACCATTTCGTTCAGGAATTCAAGAGAAAGCACAAGAAGGACATCAGTGGAAATCCGAGAGCTCTTAGGAGATTGAGGACTGCTTGTGAGAGGGCCAAGAGGACCCTTTCATCCACTGCCCAAACTACAATCGAGATTGATTCTTTGTATGAGGGAATTGATTTCTATACAACCATCACTCGTGCTAGATTCGAGGAGCTCAACATGGACTTGTTCCGAAAGTGTATGGAGCCGGTTGAGAAGTGTCTGAGAGATGCAAAGATGGACAAAAGCACCGTCCATGATGTTGTCCTTGTTGGTGGCTCTACAAGGATTCCTAAGGTGCAGCAGCTATTGCAGGATTTCTTCAATGGCAAAGAACTCTGCAAAAGTATCAATCCTGATGAGGCAGTTGCTTATGGGGCTGCTGTCCAAGCGGCGATCTTGAGTGGTGAAGGAAATGAGAAAGTGCAGGATCTTCTTCTTTTGGATGTCACTCCTCTGTCTCTTGGTTTGGAAACTGCAGGAGGTGTGATGACAGTTTTGATTCCAAGGAATACCACCATACCCACCAAGAAGGAGCAAGTATTTTCCACATACTCAGACAACCAACCTGGTGTTCTCATTCAGGTCTACGAGGGTGAGAGGACAAGAACCAGAGATAATAACTTGTTGGGCAAGTTTGAACTTTCCGGCATCCCTCCGGCTCCGAGAGGTGTTCCACAGATCACTGTCTGCTTTGACATTGACGCCAACGGTATTCTAAACGTTTCTGCAGAGGACAAGACCACCGGACAGAAGAATAAGATCACCATTACCAACGACAAAGGAAGGTTGTCCAAAGAGGAGATCGAGAAACTGGTTCAGGAAGCTGAGAAATTCAAGGCCGAAGATGAGGAACACAAGAAGAAAGTTGAATCAAAGAACGCGTTAGAAAACTATGCATACAACATGAGGAACACTATCAGAGATGAAAAGATAAGTGGTAAACTTGCGCCAGCAGATAAGAAGAAGATTGAAGATGCAGTCGAACAGGCCGTCCAGTGGCTAGACAGCAACCAGCTGGCTGAGTCGGATGAATTTGAAGACAAGATGAAGGAACTTGAATCTATATGCAATCCCATCATAGCCAAGATGTATCAAGGTGGTGCTGACATGGGCGGCAGCGCAATGGATGATGATGCTCCTCCTTCTGGCGGCAGCGGCGCTGGTCCTAAGATTGAAGAAGTTGATTAA

Protein sequence

MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKAEDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLAESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD
Homology
BLAST of Spg039625 vs. NCBI nr
Match: KAG6602369.1 (Heat shock cognate 70 kDa protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 914.4 bits (2362), Expect = 3.9e-262
Identity = 469/475 (98.74%), Postives = 471/475 (99.16%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLD NQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG AMDDD PPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGGAMDDDTPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. NCBI nr
Match: XP_022989947.1 (probable mediator of RNA polymerase II transcription subunit 37c [Cucurbita maxima] >XP_023532762.1 probable mediator of RNA polymerase II transcription subunit 37c [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914.4 bits (2362), Expect = 3.9e-262
Identity = 469/475 (98.74%), Postives = 471/475 (99.16%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLD NQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG AMDDD PPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGGAMDDDTPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. NCBI nr
Match: XP_022957133.1 (probable mediator of RNA polymerase II transcription subunit 37c [Cucurbita moschata])

HSP 1 Score: 913.7 bits (2360), Expect = 6.7e-262
Identity = 469/475 (98.74%), Postives = 471/475 (99.16%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLD NQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG AMDDD PPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGVAMDDDTPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. NCBI nr
Match: XP_004142749.1 (heat shock cognate 70 kDa protein [Cucumis sativus] >KGN51294.1 hypothetical protein Csa_009156 [Cucumis sativus])

HSP 1 Score: 913.7 bits (2360), Expect = 6.7e-262
Identity = 468/475 (98.53%), Postives = 474/475 (99.79%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLDSNQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDSNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG+AM+DDAPPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGAAMNDDAPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. NCBI nr
Match: XP_008458892.1 (PREDICTED: heat shock cognate 70 kDa protein [Cucumis melo] >KAA0042494.1 heat shock cognate 70 kDa protein [Cucumis melo var. makuwa] >TYK22880.1 heat shock cognate 70 kDa protein [Cucumis melo var. makuwa])

HSP 1 Score: 912.9 bits (2358), Expect = 1.1e-261
Identity = 468/475 (98.53%), Postives = 474/475 (99.79%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQA+QWLDSNQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAIQWLDSNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMG +AM+DDAPPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGSAAMNDDAPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 2.7e-250
Identity = 442/477 (92.66%), Postives = 463/477 (97.06%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+KKDI+GNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           +EGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 FEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQ+FSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEK+VQEAEK+KA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKV++KNALENYAYNMRNTI+DEKI+ KL  ADKKKIEDA++QA++WLD NQLA
Sbjct: 534 EDEEHKKKVDAKNALENYAYNMRNTIKDEKIASKLDAADKKKIEDAIDQAIEWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQG-GADMGGS-AMDDDAPPSGGSGAGPKIEEVD 476
           E+DEFEDKMKELES+CNPIIA+MYQG G DMGG+  MDDD P  G  GAGPKIEEVD
Sbjct: 594 EADEFEDKMKELESLCNPIIARMYQGAGPDMGGAGGMDDDTPAGGSGGAGPKIEEVD 650

BLAST of Spg039625 vs. ExPASy Swiss-Prot
Match: P22953 (Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3)

HSP 1 Score: 857.8 bits (2215), Expect = 5.7e-248
Identity = 442/479 (92.28%), Postives = 461/479 (96.24%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK KKDI+GNPRALRRLRT+CERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFY+TITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQ AILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMT LIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSK+EIEK+VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVE+KNALENYAYNMRNTI+DEKI  KL  ADKKKIED++EQA+QWL+ NQLA
Sbjct: 534 EDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQG-GADMGG---SAMDDDAPPSGGSGAGPKIEEVD 476
           E+DEFEDKMKELESICNPIIAKMYQG G + GG   S MDDDAPP+ G GAGPKIEEVD
Sbjct: 594 EADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMDDDAPPASG-GAGPKIEEVD 651

BLAST of Spg039625 vs. ExPASy Swiss-Prot
Match: P27322 (Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum OX=4081 GN=HSC-2 PE=2 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 9.7e-248
Identity = 438/475 (92.21%), Postives = 457/475 (96.21%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+KKDI+GNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFY+TITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANG LNVSAEDKTTGQKNKITITNDKGRLSKEEIEK+VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGTLNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVE+KNALENYAYNMRNTI+DEKI+ KL+  D+ KIEDA+EQA+QWLD NQLA
Sbjct: 534 EDEEHKKKVEAKNALENYAYNMRNTIKDEKIASKLSADDRTKIEDAIEQAIQWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           E++EFEDKMKELES+CNPIIAKMYQG     G  MDD+ P   G GAGPKIEEVD
Sbjct: 594 EAEEFEDKMKELESLCNPIIAKMYQG----AGGDMDDEGPAPSGGGAGPKIEEVD 644

BLAST of Spg039625 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 2.4e-246
Identity = 438/480 (91.25%), Postives = 463/480 (96.46%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASS GEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+KKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFY+TITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           G MTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GGMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIE++VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIERMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEE KKKVE+KNALENYAYNMRNTI+D+KI+ +L+ ADKK+IEDA+++A++WLD+NQLA
Sbjct: 534 EDEELKKKVEAKNALENYAYNMRNTIKDDKINSQLSAADKKRIEDAIDEAIKWLDNNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGS-----GAGPKIEEVD 476
           E+DEFEDKMKELESICNPIIAKMYQGGA  GG+ MD+D P  GGS     GAGPKIEEVD
Sbjct: 594 EADEFEDKMKELESICNPIIAKMYQGGA--GGATMDEDGPSVGGSAGSQTGAGPKIEEVD 651

BLAST of Spg039625 vs. ExPASy Swiss-Prot
Match: P24629 (Heat shock cognate 70 kDa protein 1 OS=Solanum lycopersicum OX=4081 GN=HSC-I PE=2 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 9.4e-243
Identity = 434/477 (90.99%), Postives = 453/477 (94.97%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+S GEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFV EFKRKHKKDI+GNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVHEFKRKHKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEG+DFY+TITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGVDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 353

Query: 181 QL-LQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETA 240
           Q+ + +FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETA
Sbjct: 354 QVAMTNFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETA 413

Query: 241 GGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGE-RTRTRDNNLLGKFELSGIP 300
           GGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQV+EGE R RTRDNNLLGKFELS IP
Sbjct: 414 GGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVFEGERRARTRDNNLLGKFELSVIP 473

Query: 301 PAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKF 360
           PAPR VPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEK+VQEAEK+
Sbjct: 474 PAPRVVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKY 533

Query: 361 KAEDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQ 420
           KAEDEE KKKVE+KN+LENYAYNMRNT++DEKI  KL+  DKKKIEDAV+QA+ WL+SNQ
Sbjct: 534 KAEDEELKKKVEAKNSLENYAYNMRNTVKDEKIGSKLSSDDKKKIEDAVDQAISWLESNQ 593

Query: 421 LAESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           LAE DEFEDKMKELE ICNPIIAKMYQG     G  MDDDAPPSGGS AGPKIEEVD
Sbjct: 594 LAEVDEFEDKMKELEGICNPIIAKMYQGAGGDAGVPMDDDAPPSGGSSAGPKIEEVD 650

BLAST of Spg039625 vs. ExPASy TrEMBL
Match: A0A6J1JH81 (probable mediator of RNA polymerase II transcription subunit 37c OS=Cucurbita maxima OX=3661 GN=LOC111486983 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 1.9e-262
Identity = 469/475 (98.74%), Postives = 471/475 (99.16%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLD NQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG AMDDD PPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGGAMDDDTPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. ExPASy TrEMBL
Match: A0A0A0KQW6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G514500 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.2e-262
Identity = 468/475 (98.53%), Postives = 474/475 (99.79%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLDSNQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDSNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG+AM+DDAPPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGAAMNDDAPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. ExPASy TrEMBL
Match: A0A6J1GZC8 (probable mediator of RNA polymerase II transcription subunit 37c OS=Cucurbita moschata OX=3662 GN=LOC111458604 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.2e-262
Identity = 469/475 (98.74%), Postives = 471/475 (99.16%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKI GKLAPADKKKIEDAVEQA+QWLD NQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKIGGKLAPADKKKIEDAVEQAIQWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMGG AMDDD PPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGGVAMDDDTPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. ExPASy TrEMBL
Match: A0A5D3DHM6 (Heat shock cognate 70 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold334G00180 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.5e-262
Identity = 468/475 (98.53%), Postives = 474/475 (99.79%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQA+QWLDSNQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAIQWLDSNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMG +AM+DDAPPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGSAAMNDDAPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. ExPASy TrEMBL
Match: A0A1S3C8G2 (heat shock cognate 70 kDa protein OS=Cucumis melo OX=3656 GN=LOC103498164 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.5e-262
Identity = 468/475 (98.53%), Postives = 474/475 (99.79%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQA+QWLDSNQLA
Sbjct: 534 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAIQWLDSNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           ESDEFEDKMKELESICNPIIAKMYQGGADMG +AM+DDAPPSGGSGAGPKIEEVD
Sbjct: 594 ESDEFEDKMKELESICNPIIAKMYQGGADMGSAAMNDDAPPSGGSGAGPKIEEVD 648

BLAST of Spg039625 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 865.5 bits (2235), Expect = 1.9e-251
Identity = 442/477 (92.66%), Postives = 463/477 (97.06%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+KKDI+GNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           +EGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKS+VHDVVLVGGSTRIPKVQ
Sbjct: 294 FEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLIPRNTTIPTKKEQ+FSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEK+VQEAEK+KA
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKA 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKV++KNALENYAYNMRNTI+DEKI+ KL  ADKKKIEDA++QA++WLD NQLA
Sbjct: 534 EDEEHKKKVDAKNALENYAYNMRNTIKDEKIASKLDAADKKKIEDAIDQAIEWLDGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQG-GADMGGS-AMDDDAPPSGGSGAGPKIEEVD 476
           E+DEFEDKMKELES+CNPIIA+MYQG G DMGG+  MDDD P  G  GAGPKIEEVD
Sbjct: 594 EADEFEDKMKELESLCNPIIARMYQGAGPDMGGAGGMDDDTPAGGSGGAGPKIEEVD 650

BLAST of Spg039625 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 857.8 bits (2215), Expect = 4.0e-249
Identity = 442/479 (92.28%), Postives = 461/479 (96.24%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK KKDI+GNPRALRRLRT+CERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           YEGIDFY+TITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ
Sbjct: 294 YEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQ AILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMT LIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSK+EIEK+VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVE+KNALENYAYNMRNTI+DEKI  KL  ADKKKIED++EQA+QWL+ NQLA
Sbjct: 534 EDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQG-GADMGG---SAMDDDAPPSGGSGAGPKIEEVD 476
           E+DEFEDKMKELESICNPIIAKMYQG G + GG   S MDDDAPP+ G GAGPKIEEVD
Sbjct: 594 EADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMDDDAPPASG-GAGPKIEEVD 651

BLAST of Spg039625 vs. TAIR 10
Match: AT3G09440.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 838.2 bits (2164), Expect = 3.3e-243
Identity = 432/477 (90.57%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+KKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           ++GIDFY  ITRARFEELN+DLFRKCMEPVEKCLRDAKMDK+++ DVVLVGGSTRIPKVQ
Sbjct: 294 FDGIDFYAPITRARFEELNIDLFRKCMEPVEKCLRDAKMDKNSIDDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLL DFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLVDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLI RNTTIPTKKEQVFSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIQRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSK+EIEK+VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKV++KNALENYAYNMRNTIRDEKI  KLA  DKKKIED++E A++WL++NQLA
Sbjct: 534 EDEEHKKKVDAKNALENYAYNMRNTIRDEKIGEKLAGDDKKKIEDSIEAAIEWLEANQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSA--MDDDAPPSGGSGAGPKIEEVD 476
           E DEFEDKMKELESICNPIIAKMYQGG   G +A  MD+D PPS G GAGPKIEEVD
Sbjct: 594 ECDEFEDKMKELESICNPIIAKMYQGGEAGGPAAGGMDEDVPPSAG-GAGPKIEEVD 649

BLAST of Spg039625 vs. TAIR 10
Match: AT3G09440.2 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 838.2 bits (2164), Expect = 3.3e-243
Identity = 432/477 (90.57%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           MRIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 MRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+KKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           ++GIDFY  ITRARFEELN+DLFRKCMEPVEKCLRDAKMDK+++ DVVLVGGSTRIPKVQ
Sbjct: 294 FDGIDFYAPITRARFEELNIDLFRKCMEPVEKCLRDAKMDKNSIDDVVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLL DFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLVDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMTVLI RNTTIPTKKEQVFSTYSDNQPGVLIQVYEGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTVLIQRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSK+EIEK+VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKV++KNALENYAYNMRNTIRDEKI  KLA  DKKKIED++E A++WL++NQLA
Sbjct: 534 EDEEHKKKVDAKNALENYAYNMRNTIRDEKIGEKLAGDDKKKIEDSIEAAIEWLEANQLA 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGADMGGSA--MDDDAPPSGGSGAGPKIEEVD 476
           E DEFEDKMKELESICNPIIAKMYQGG   G +A  MD+D PPS G GAGPKIEEVD
Sbjct: 594 ECDEFEDKMKELESICNPIIAKMYQGGEAGGPAAGGMDEDVPPSAG-GAGPKIEEVD 649

BLAST of Spg039625 vs. TAIR 10
Match: AT5G02490.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 836.6 bits (2160), Expect = 9.6e-243
Identity = 426/480 (88.75%), Postives = 452/480 (94.17%), Query Frame = 0

Query: 1   MRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 60
           +RIINEPTAAAIAYGLDKKA+SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH
Sbjct: 174 LRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTH 233

Query: 61  LGGEDFDNRLVNHFVQEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSL 120
           LGGEDFDNR+VNHFVQEFKRK+K+DI+G PRALRRLRTACERAKRTLSSTAQTTIEIDSL
Sbjct: 234 LGGEDFDNRMVNHFVQEFKRKNKQDITGQPRALRRLRTACERAKRTLSSTAQTTIEIDSL 293

Query: 121 YEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQ 180
           Y G DFY+ ITRARFEE+NMDLFRKCMEPVEKCLRDAKMDKSTVH++VLVGGSTRIPKVQ
Sbjct: 294 YGGADFYSPITRARFEEMNMDLFRKCMEPVEKCLRDAKMDKSTVHEIVLVGGSTRIPKVQ 353

Query: 181 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 240
           QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG
Sbjct: 354 QLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAG 413

Query: 241 GVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPA 300
           GVMT LI RNTTIPTKKEQVFSTYSDNQPGVLIQV+EGER RT+DNNLLGKFELSGIPPA
Sbjct: 414 GVMTTLIQRNTTIPTKKEQVFSTYSDNQPGVLIQVFEGERARTKDNNLLGKFELSGIPPA 473

Query: 301 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKLVQEAEKFKA 360
           PRGVPQITVCFDIDANGILNVSAEDKTTG+KNKITITNDKGRLSKE+IEK+VQEAEK+K+
Sbjct: 474 PRGVPQITVCFDIDANGILNVSAEDKTTGKKNKITITNDKGRLSKEDIEKMVQEAEKYKS 533

Query: 361 EDEEHKKKVESKNALENYAYNMRNTIRDEKISGKLAPADKKKIEDAVEQAVQWLDSNQLA 420
           EDEEHKKKVE+KNALENYAYNMRNTIRDEKI  KL  ADKKK+ED++E+A+QWLD NQL 
Sbjct: 534 EDEEHKKKVEAKNALENYAYNMRNTIRDEKIGEKLPAADKKKVEDSIEEAIQWLDGNQLG 593

Query: 421 ESDEFEDKMKELESICNPIIAKMYQGGA-----DMGGSAMDDDAPPSGGSGAGPKIEEVD 476
           E+DEFEDKMKELES+CNPIIAKMYQGGA       G S MD+D  P    GAGPKIEEVD
Sbjct: 594 EADEFEDKMKELESVCNPIIAKMYQGGAGGEAGGPGASGMDEDEAPPASGGAGPKIEEVD 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6602369.13.9e-26298.74Heat shock cognate 70 kDa protein, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022989947.13.9e-26298.74probable mediator of RNA polymerase II transcription subunit 37c [Cucurbita maxi... [more]
XP_022957133.16.7e-26298.74probable mediator of RNA polymerase II transcription subunit 37c [Cucurbita mosc... [more]
XP_004142749.16.7e-26298.53heat shock cognate 70 kDa protein [Cucumis sativus] >KGN51294.1 hypothetical pro... [more]
XP_008458892.11.1e-26198.53PREDICTED: heat shock cognate 70 kDa protein [Cucumis melo] >KAA0042494.1 heat s... [more]
Match NameE-valueIdentityDescription
Q9LHA82.7e-25092.66Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1[more]
P229535.7e-24892.28Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3[more]
P273229.7e-24892.21Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum OX=4081 GN=HSC-2 PE=... [more]
P091892.4e-24691.25Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1[more]
P246299.4e-24390.99Heat shock cognate 70 kDa protein 1 OS=Solanum lycopersicum OX=4081 GN=HSC-I PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1JH811.9e-26298.74probable mediator of RNA polymerase II transcription subunit 37c OS=Cucurbita ma... [more]
A0A0A0KQW63.2e-26298.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G514500 PE=3 SV=1[more]
A0A6J1GZC83.2e-26298.74probable mediator of RNA polymerase II transcription subunit 37c OS=Cucurbita mo... [more]
A0A5D3DHM65.5e-26298.53Heat shock cognate 70 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C8G25.5e-26298.53heat shock cognate 70 kDa protein OS=Cucumis melo OX=3656 GN=LOC103498164 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G12580.11.9e-25192.66heat shock protein 70 [more]
AT5G02500.14.0e-24992.28heat shock cognate protein 70-1 [more]
AT3G09440.13.3e-24390.57Heat shock protein 70 (Hsp 70) family protein [more]
AT3G09440.23.3e-24390.57Heat shock protein 70 (Hsp 70) family protein [more]
AT5G02490.19.6e-24388.75Heat shock protein 70 (Hsp 70) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 341..368
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 36..46
score: 79.84
coord: 196..216
score: 81.15
coord: 223..242
score: 83.09
coord: 304..320
score: 76.99
coord: 164..180
score: 77.28
NoneNo IPR availableGENE3D3.30.420.40coord: 1..211
e-value: 2.2E-102
score: 344.5
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 63..145
e-value: 2.2E-102
score: 344.5
NoneNo IPR availableGENE3D3.30.420.40coord: 19..193
e-value: 2.2E-102
score: 344.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 446..475
NoneNo IPR availablePANTHERPTHR19375:SF424HEAT SHOCK COGNATE 70 KDA PROTEIN 2-LIKEcoord: 1..455
IPR029048Heat shock protein 70kD, C-terminal domain superfamilyGENE3D1.20.1270.10coord: 372..475
e-value: 2.4E-41
score: 142.0
IPR029048Heat shock protein 70kD, C-terminal domain superfamilySUPERFAMILY100934Heat shock protein 70kD (HSP70), C-terminal subdomaincoord: 370..450
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 1..445
e-value: 9.3E-179
score: 595.4
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 1..455
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 218..371
e-value: 1.2E-77
score: 261.1
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 219..376
IPR018181Heat shock protein 70, conserved sitePROSITEPS01036HSP70_3coord: 167..181
IPR018181Heat shock protein 70, conserved sitePROSITEPS00329HSP70_2coord: 30..43
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 25..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039625.1Spg039625.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone
molecular_function GO:0051082 unfolded protein binding