Spg039520 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg039520
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold10: 43959993 .. 43962240 (-)
RNA-Seq Expression	Spg039520
Synteny	Spg039520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAATGGCAAACTTTTCAGCATTCTTTGTTCTTGTTTTGGCCATTTTTGTTCAATCTCTACAAATTTCAGCCAAAATTTCTTTCAATGTGCTCGATTATGGAGCTGTTGGGGATGGAACTATTGAAGATACTCAAGTAAACACGATGGTTTATTTTTTTTTTTTCCTTTTATCTCTATTTTGATATGAATTGTTTGATCAACAGGCTTTTCTTGATGCTTGGGAAGCTACATGCAAGTCTTCAGATTCTTCTCCTGCAATGGTGGTTCCCAGTGGCAAATCATTTTTACTTCAGCCTTTCACTTTGGATGGTCGCCATTGCAAGTCTAGAAATATTAATATTGTGGTTTGGATTCGTTTCGATATGATTTAATTTTGTTATGCTTTATAATTTTCAATTTTAGTCCAATAATTGCAAGTGTGAGATAAGTTTTGATCTTTCTATATATAAATATTTTTACCTATTAATTTTTTTTGTTTCGAATTAGATTGATGGAGAATTGACTGCACCAAAAGGTCCATGGGCATGGAAATGTAAAGGAAAAAAATGTCGTCAATGGATTTTATTTAACCACATGAAGGGTCTTAAAATTCAGGGTTCTGGCTCTATCAATGGTCAAGGAACCACTTGGTGGCATCTTTCTTGCAAAGATAATAAAAGGGTATATATTTTTTTTTATGAAATATATTCATTGATTTTTTTTTTTAATTTAAATTTTTTGGATGAGTTATTAATAAATCTGTTTGATTTTGTATTTGAGTTATTTATCAATTTTTTAATAGTGAAATTTCAATTTAGATTTGATTCTGTGTTGATATTTGGTCATTCTTTGGCAGGTTTGTGGAAGAAAGCCAACTGTAAGTAATCATTAATTATTTTTCTAAAATTAGTATTTGATTCCAATTAATTAAATTAAATAATTTTTATGCTTACAATAATCCTTTTGAAGCAACATGCATGTTTGTAAGTTTATGATTGATAACTGAGAGACAACTTTTAGGGCTTTATTTTGGAGCATTCGGAGAATGTCCACATGAGTGGTCTAACTTTTAAGGATAGTCCTCAAATGCATATAGCATTGGAAAGATCAAAATATATATACATTGCAAATTTACACATTCAAGCACCAGACGACAGTCCTAATACGGATGGAATTCATATTCAAAATTCTCATAAAATCTCCATTCGTAAATCCGACATTGCCACAGGTAACACACCCATCTCAACTAATTTTATAATATTTTCTATTAGTTTTCTCTACTAATTAATTGGAGTATTGAGTTTTTACAGGGGATGATTGTATTTCAATTGGGGATGGTTCAGCTAAAATCAGTATCTCCAACATTGTCTGTGGTCCAGGTCATGGCATTAGGTGATCATGGATGTTTAATTTATCGTTAACCTAATCAAATTCAACATTGATTTTTCTCTAAAAGTTCCAAATTATTTTTCTTTTTTTTTTTCCAGCATCGGAAGCCTTGGAAAACATGGCCGAAGAGAAACAGTTGAACAAGTTCACGTGAAGGATGTAACTTTTACGGGTACCACTAATGGAGCTAGAATAAAAACATGGCAGGTAATTATTAAATCAATTAATGAATGGATGTTATAACTTATAAGTTTATATAATTTGTATTTAGACTATCTTATAAATTTCAAATTGATCTTATTATTATATTGTGTGATTTTTTTTATCAATTTAGTGGATTGAAAAGTTTATGGTCTAAATTTCTAATTAATTTTGTAAATGATGGTGATGTGATTTAGGGTGGGAGTGGATATGCAAGAAACATCGCATTCGAAGGCATAATTTCTCGTGGCTCGTCAAATCCAATCATCATCGATCAATTTTACTGCGATCATGAAAAATGCAAAAACCAAGTAAATTTAATAATCTCCCTTTCTACATTTTGGCATTATTTACGATTAATTCATCAATCTGCAACAATGTTTTCGAAACAAAATCAAATGGAAATATATATATTTTGTGCAGACTTCAGCGGTAAAGATCAGCAATGTATTGTACAGACAAATATGGGGAACATCAAAGAGTAAAATTGCAGTGCATTTTGCTTGCAGTGACGCAATCCCTTGTAGAAATATCTTGATGAAGGATATTGAGTTGAGACCTATTGGTGATGATGGAGATGAGGAAACGTCGTCGTATTGTGGGAATGTCGTTGATGGTCGTAGGAATGGGACTGTGATTCCCAGTGTCCCTTGCTTAAACGAAATTAATCAGTAG

mRNA sequence

ATGCAAATGGCAAACTTTTCAGCATTCTTTGTTCTTGTTTTGGCCATTTTTGTTCAATCTCTACAAATTTCAGCCAAAATTTCTTTCAATGTGCTCGATTATGGAGCTGTTGGGGATGGAACTATTGAAGATACTCAAGCTTTTCTTGATGCTTGGGAAGCTACATGCAAGTCTTCAGATTCTTCTCCTGCAATGGTGGTTCCCAGTGGCAAATCATTTTTACTTCAGCCTTTCACTTTGGATGGTCGCCATTGCAAGTCTAGAAATATTAATATTGTGGTTTGTGGAAGAAAGCCAACTGGCTTTATTTTGGAGCATTCGGAGAATGTCCACATGAGTGGTCTAACTTTTAAGGATAGTCCTCAAATGCATATAGCATTGGAAAGATCAAAATATATATACATTGCAAATTTACACATTCAAGCACCAGACGACAGTCCTAATACGGATGGAATTCATATTCAAAATTCTCATAAAATCTCCATTCGTAAATCCGACATTGCCACAGGGGATGATTGTATTTCAATTGGGGATGGTTCAGCTAAAATCAGTATCTCCAACATTGTCTGTGGTCCAGGTCATGGCATTAGCATCGGAAGCCTTGGAAAACATGGCCGAAGAGAAACAGTTGAACAAGTTCACGTGAAGGATGTAACTTTTACGGGTACCACTAATGGAGCTAGAATAAAAACATGGCAGGGTGGGAGTGGATATGCAAGAAACATCGCATTCGAAGGCATAATTTCTCGTGGCTCGTCAAATCCAATCATCATCGATCAATTTTACTGCGATCATGAAAAATGCAAAAACCAAACTTCAGCGGTAAAGATCAGCAATGTATTGTACAGACAAATATGGGGAACATCAAAGAGTAAAATTGCAGTGCATTTTGCTTGCAGTGACGCAATCCCTTGTAGAAATATCTTGATGAAGGATATTGAGTTGAGACCTATTGGTGATGATGGAGATGAGGAAACGTCGTCGTATTGTGGGAATGTCGTTGATGGTCGTAGGAATGGGACTGTGATTCCCAGTGTCCCTTGCTTAAACGAAATTAATCAGTAG

Coding sequence (CDS)

ATGCAAATGGCAAACTTTTCAGCATTCTTTGTTCTTGTTTTGGCCATTTTTGTTCAATCTCTACAAATTTCAGCCAAAATTTCTTTCAATGTGCTCGATTATGGAGCTGTTGGGGATGGAACTATTGAAGATACTCAAGCTTTTCTTGATGCTTGGGAAGCTACATGCAAGTCTTCAGATTCTTCTCCTGCAATGGTGGTTCCCAGTGGCAAATCATTTTTACTTCAGCCTTTCACTTTGGATGGTCGCCATTGCAAGTCTAGAAATATTAATATTGTGGTTTGTGGAAGAAAGCCAACTGGCTTTATTTTGGAGCATTCGGAGAATGTCCACATGAGTGGTCTAACTTTTAAGGATAGTCCTCAAATGCATATAGCATTGGAAAGATCAAAATATATATACATTGCAAATTTACACATTCAAGCACCAGACGACAGTCCTAATACGGATGGAATTCATATTCAAAATTCTCATAAAATCTCCATTCGTAAATCCGACATTGCCACAGGGGATGATTGTATTTCAATTGGGGATGGTTCAGCTAAAATCAGTATCTCCAACATTGTCTGTGGTCCAGGTCATGGCATTAGCATCGGAAGCCTTGGAAAACATGGCCGAAGAGAAACAGTTGAACAAGTTCACGTGAAGGATGTAACTTTTACGGGTACCACTAATGGAGCTAGAATAAAAACATGGCAGGGTGGGAGTGGATATGCAAGAAACATCGCATTCGAAGGCATAATTTCTCGTGGCTCGTCAAATCCAATCATCATCGATCAATTTTACTGCGATCATGAAAAATGCAAAAACCAAACTTCAGCGGTAAAGATCAGCAATGTATTGTACAGACAAATATGGGGAACATCAAAGAGTAAAATTGCAGTGCATTTTGCTTGCAGTGACGCAATCCCTTGTAGAAATATCTTGATGAAGGATATTGAGTTGAGACCTATTGGTGATGATGGAGATGAGGAAACGTCGTCGTATTGTGGGAATGTCGTTGATGGTCGTAGGAATGGGACTGTGATTCCCAGTGTCCCTTGCTTAAACGAAATTAATCAGTAG

Protein sequence

MQMANFSAFFVLVLAIFVQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLDGRHCKSRNINIVVCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDEETSSYCGNVVDGRRNGTVIPSVPCLNEINQ

Homology

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BLAST of Spg039520 vs. NCBI nr
Match: XP_022133622.1 (probable polygalacturonase At1g80170 [Momordica charantia])

HSP 1 Score: 525.8 bits (1353), Expect = 2.9e-145
Identity = 271/408 (66.42%), Postives = 304/408 (74.51%), Query Frame = 0


Query: 3   MANFSAFFVLVLAIFVQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKS-SDS 62
           MANF A FVLVLAIF Q  QI +  +FNV++YGAVG+G  EDTQAFL+AW+ATCKS S S
Sbjct: 1   MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS 60

Query: 63  SPAMVVPSGKSFLLQPFTLDGRHCKSRNINIV---------------------------- 122
           SP MVVP GKSFLLQPFT +GR CKS NI IV                            
Sbjct: 61  SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 120

Query: 123 ------------------------------VCGRKPTGFILEHSENVHMSGLTFKDSPQM 182
                                         VCG+KPTGFILEHSE V + GLTFKDSPQM
Sbjct: 121 HMKGLKIQGSGTINGRGSTWWRLSCTQNKRVCGKKPTGFILEHSEKVQIKGLTFKDSPQM 180

Query: 183 HIALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKI 242
           H+A  RS+ +Y  NLHIQAP +SPNTDGIHIQNS +I IR S I TGDDCISIGDGS++I
Sbjct: 181 HMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRI 240

Query: 243 SISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIA 302
           SISNI CGPGHGISIGSLGKHG+RETVE +HV+DVTFTGTTNGARIKTWQGG GYAR+I+
Sbjct: 241 SISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHIS 300

Query: 303 FEGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAI 352
           FEGI SRGSSNPI+IDQFYCDHE+CKNQTSAVKISNVLYR+I GTS S+IAV  ACSD+I
Sbjct: 301 FEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSI 360

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BLAST of Spg039520 vs. NCBI nr
Match: XP_038889205.1 (probable polygalacturonase At1g80170 [Benincasa hispida])

HSP 1 Score: 495.4 bits (1274), Expect = 4.2e-136
Identity = 248/385 (64.42%), Postives = 286/385 (74.29%), Query Frame = 0


Query: 28  SFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLDGRHCKS 87
           +FNV++YGA+G+G+IEDTQAFLDAW+ATC SSDSSP M VP G SFLLQPFTLDG +CKS
Sbjct: 34  TFNVVNYGAIGNGSIEDTQAFLDAWKATCNSSDSSPIMTVPGGNSFLLQPFTLDGSNCKS 93

Query: 88  RNINIVV----------------------------------------------------- 147
            +I+IV+                                                     
Sbjct: 94  TSISIVIDGELIAPKNPSAWKYKGKECRQWILLKHMKGLVIKGSGTINGRGNTWWHLSCK 153

Query: 148 ------CGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDDSPN 207
                 C RKPTG ILEHSE VH+  L FKDSPQMH+A+ERSKY+Y+ NL+IQAP +SPN
Sbjct: 154 NKNKRCCRRKPTGLILEHSEKVHIKDLAFKDSPQMHMAIERSKYVYVKNLNIQAPSESPN 213

Query: 208 TDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHGRRE 267
           TDGIHIQ+S KI I  S+I TGDDCISIGDGS +I+IS+I CGPGHGISIGSLGKHG  E
Sbjct: 214 TDGIHIQHSRKIVISMSNIRTGDDCISIGDGSYRINISSIACGPGHGISIGSLGKHGGHE 273

Query: 268 TVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCDHEKC 327
           TVE V+VKDV FTGTTNGARIKTWQGGSGYARNI FEGIISRGSSNPIIIDQFYCDHEKC
Sbjct: 274 TVEFVNVKDVIFTGTTNGARIKTWQGGSGYARNINFEGIISRGSSNPIIIDQFYCDHEKC 333

Query: 328 KNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDEETSS 354
           KNQTSAVKISNV+Y +I GTS+SKIAV+ ACS+++PCRNI MKDIEL  I +D  + TSS
Sbjct: 334 KNQTSAVKISNVVYSKISGTSRSKIAVNLACSESVPCRNIFMKDIELGSIHEDEQQITSS 393

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BLAST of Spg039520 vs. NCBI nr
Match: KGN52445.1 (hypothetical protein Csa_008476 [Cucumis sativus])

HSP 1 Score: 485.3 bits (1248), Expect = 4.4e-133
Identity = 252/409 (61.61%), Postives = 289/409 (70.66%), Query Frame = 0


Query: 10  FVLVLAIFVQSLQI-SAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDS--SPAMV 69
           F L+L I +Q LQI S+  +FNVLDYGAVGDG  EDTQAFLDAW+A CKSS+S  SP M+
Sbjct: 4   FFLILIISLQFLQINSSSKTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPIML 63

Query: 70  VPSGKSFLLQPFTLDGRHCKSRNINIV--------------------------------- 129
           VP GKSFLLQPFTLDG  CKS   NI+                                 
Sbjct: 64  VPPGKSFLLQPFTLDGYDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKQCRQWILLKHM 123

Query: 130 ---------------------------VCGRKPTGFILEHSENVHMSGLTFKDSPQMHIA 189
                                      VC R+PT  ILE SE VH+  + FKDSPQMH+A
Sbjct: 124 KGLVVKGSGTINAQGNTWWDLCTNEEKVCTRRPTSLILEDSEKVHIKNIAFKDSPQMHMA 183

Query: 190 LERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISIS 249
           +E SKY+++ NL+I+AP DSPNTDGIHIQ+S KISI  S I TGDDCISIGDGS +I+IS
Sbjct: 184 IENSKYVFVKNLYIEAPGDSPNTDGIHIQHSRKISITSSSIRTGDDCISIGDGSYRINIS 243

Query: 250 NIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEG 309
            I CGPGHGISIGSLGKHG  E VE +HVKDVTFT TTNG RIKTWQGGSGYARNI FEG
Sbjct: 244 KIACGPGHGISIGSLGKHGNHEKVEFIHVKDVTFTDTTNGVRIKTWQGGSGYARNIIFEG 303

Query: 310 IISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCR 354
           I+SRGS NPIIIDQFYCDHEKCKNQTSAVK+SNVLY ++ GTSKSKIAV+ ACS+  PCR
Sbjct: 304 IVSRGSLNPIIIDQFYCDHEKCKNQTSAVKVSNVLYNRVSGTSKSKIAVNLACSETEPCR 363

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BLAST of Spg039520 vs. NCBI nr
Match: XP_031741769.1 (probable polygalacturonase At1g80170, partial [Cucumis sativus])

HSP 1 Score: 476.5 bits (1225), Expect = 2.0e-130
Identity = 242/388 (62.37%), Postives = 275/388 (70.88%), Query Frame = 0


Query: 30  NVLDYGAVGDGTIEDTQAFLDAWEATCKSSDS--SPAMVVPSGKSFLLQPFTLDGRHCKS 89
           NVLDYGAVGDG  EDTQAFLDAW+A CKSS+S  SP M+VP GKSFLLQPFTLDG  CKS
Sbjct: 1   NVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPIMLVPPGKSFLLQPFTLDGYDCKS 60

Query: 90  RNINIV------------------------------------------------------ 149
              NI+                                                      
Sbjct: 61  TKTNIISIVIDGELIAPKDRWAWKYEGKQCRQWILLKHMKGLVVKGSGTINAQGNTWWDL 120

Query: 150 ------VCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDDSP 209
                 VC R+PT  ILE SE VH+  + FKDSPQMH+A+E SKY+++ NL+I+AP DSP
Sbjct: 121 CTNEEKVCTRRPTSLILEDSEKVHIKNIAFKDSPQMHMAIENSKYVFVKNLYIEAPGDSP 180

Query: 210 NTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHGRR 269
           NTDGIHIQ+S KISI  S I TGDDCISIGDGS +I+IS I CGPGHGISIGSLGKHG  
Sbjct: 181 NTDGIHIQHSRKISITSSSIRTGDDCISIGDGSYRINISKIACGPGHGISIGSLGKHGNH 240

Query: 270 ETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCDHEK 329
           E VE +HVKDVTFT TTNG RIKTWQGGSGYARNI FEGI+SRGS NPIIIDQFYCDHEK
Sbjct: 241 EKVEFIHVKDVTFTDTTNGVRIKTWQGGSGYARNIIFEGIVSRGSLNPIIIDQFYCDHEK 300

Query: 330 CKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDEE-- 354
           CKNQTSAVK+SNVLY ++ GTSKSKIAV+ ACS+  PCRNILMKDI+L+ I +D +EE  
Sbjct: 301 CKNQTSAVKVSNVLYNRVSGTSKSKIAVNLACSETEPCRNILMKDIKLKSIIEDEEEEDI 360

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BLAST of Spg039520 vs. NCBI nr
Match: XP_016902407.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 471.9 bits (1213), Expect = 5.0e-129
Identity = 249/412 (60.44%), Postives = 284/412 (68.93%), Query Frame = 0


Query: 10  FVLVLAIFVQSLQISAKIS---FNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDS--SPA 69
           FVL+L   ++ LQI++  S   FNVLDYGAVGDG  EDTQAFLDAW+A CKSS+S  SP 
Sbjct: 4   FVLILITSLRFLQINSSASSQTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPI 63

Query: 70  MVVPSGKSFLLQPFTLDGRHCKSRNINIV------------------------------- 129
           M+VP GKSFLLQPFTLDG  CKS   NI+                               
Sbjct: 64  MLVPGGKSFLLQPFTLDGHDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKECRQWILLK 123

Query: 130 -----------------------------VCGRKPTGFILEHSENVHMSGLTFKDSPQMH 189
                                        VC R+PT  ILE S+ VH+  L FKDSPQMH
Sbjct: 124 HMKGLVVKGSGSINAQGNSWWDLCTNEEKVCTRRPTSLILEDSDKVHIKNLAFKDSPQMH 183

Query: 190 IALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKIS 249
           +A+E SKY+YI NL+I+AP DSPNTDGIHIQ S K+SI +S I TGDDCISIGDGS +I+
Sbjct: 184 MAIESSKYVYIKNLYIEAPGDSPNTDGIHIQRSKKVSISRSSIRTGDDCISIGDGSYRIN 243

Query: 250 ISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAF 309
           IS I CGPGHGISIGSLGKHG  ETVE VHVKDVTFT TTNG RIKTWQGGSGYARNI F
Sbjct: 244 ISKIACGPGHGISIGSLGKHGDHETVEFVHVKDVTFTDTTNGFRIKTWQGGSGYARNIIF 303

Query: 310 EGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIP 354
           EG++SRGS NPIIIDQFYCDHEKCKN TSAVKISNV Y +I GTS+SKIAV+  CS+  P
Sbjct: 304 EGMVSRGSFNPIIIDQFYCDHEKCKNHTSAVKISNVSYNRISGTSRSKIAVNLVCSETKP 363

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BLAST of Spg039520 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.4e-71
Identity = 161/395 (40.76%), Postives = 209/395 (52.91%), Query Frame = 0


Query: 5   NFSAFFVLVLAIFVQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPA 64
           NFS FF   L IF  S       + +V  +GAVGDG  +D+QAFL AWEA C S      
Sbjct: 9   NFSLFF---LQIFTSS------NALDVTQFGAVGDGVTDDSQAFLKAWEAVC-SGTGDGQ 68

Query: 65  MVVPSGKSFLLQPFTLDGRHCKSRNINIVVCGR--------------------------- 124
            VVP+G +F+LQP    G  CKS  + + + G+                           
Sbjct: 69  FVVPAGMTFMLQPLKFQG-SCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 128

Query: 125 -------------------KPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANL 184
                              +PT        N+ +SGLT  DSP  HI +    Y+ I++L
Sbjct: 129 EGDGEINGQGSSWWEHKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSL 188

Query: 185 HIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISI 244
            I AP+ SPNTDGI +  S  + I+   IATGDDCI+I  G++ I IS I CGPGHGISI
Sbjct: 189 RINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISI 248

Query: 245 GSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIII 304
           GSLGK G   TVE V V++  F GT NGARIKTWQGGSGYAR I F GI      NPIII
Sbjct: 249 GSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIII 308

Query: 305 DQFY--CDHEKCKN-QTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIEL 350
           DQFY   D +  K+ ++SAV++S V++    GTSKS+  V F CS+ +PC  I ++D+++
Sbjct: 309 DQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKI 368

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BLAST of Spg039520 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.7e-68
Identity = 154/380 (40.53%), Postives = 196/380 (51.58%), Query Frame = 0


Query: 30  NVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLDG------- 89
           +V ++GA G+G  +DT+AF DAW+  C SS     ++VP   + LL+P  L G       
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTAC-SSKVKTRILVPENYTCLLRPIDLSGPCKARLT 113

Query: 90  --------------------------------------------------RHCKSRNINI 149
                                                             R CK  + N 
Sbjct: 114 LQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN- 173

Query: 150 VVCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDDSPNTDGI 209
             C   PT       +N+ +  L   DS QMHIAL   + + I+ L + AP  SPNTDGI
Sbjct: 174 -PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGI 233

Query: 210 HIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHGRRETVEQ 269
           HI  S  I I  + ++TGDDCISI   S +ISISNI+CGPGHGISIGSLGK    E V  
Sbjct: 234 HISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRD 293

Query: 270 VHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCDHEK-CKNQ 329
           + V     + T NG RIKTWQGGSG    I F  I     SNPIIIDQ+YCD  K C NQ
Sbjct: 294 ITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQ 353

Query: 330 TSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDEETSSYCG 352
           TSA+ I N+ +  + GTS SK A+  +CSD+ PCRNIL++DI+L P   +GD  T S+C 
Sbjct: 354 TSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEP--SNGDGFTESFCW 413

[Close]

BLAST of Spg039520 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.5e-64
Identity = 145/385 (37.66%), Postives = 197/385 (51.17%), Query Frame = 0


Query: 18  VQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQP 77
           + SL      + NVL YGA  DG+ + T+AFL AW+  C S++ +  ++VP G+ FL+  
Sbjct: 22  ISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPT-TIIVPKGR-FLVGN 81

Query: 78  FTLDGRHCKSRNINIVVCG----------------------------------------- 137
               G  CK   I+I + G                                         
Sbjct: 82  LVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLW 141

Query: 138 --------RKPTG---FILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDD 197
                     PTG    +   S N+ +SGLT  +S + HI ++ S  + I  + + A ++
Sbjct: 142 KCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADEN 201

Query: 198 SPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHG 257
           SPNTDGIH+++SH + I  S I TGDDCISIG GS  + I  I CGPGHGISIGSLG+  
Sbjct: 202 SPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAE 261

Query: 258 RRETVEQVHVKDVTFTGTTNGARIKTW-QGGSGYARNIAFEGIISRGSSNPIIIDQFYCD 317
             + V+ V V +V F GT NG RIKTW +  + +ARNI F+ I  +   NPIIIDQ YC 
Sbjct: 262 EEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCL 321

Query: 318 HEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDE 350
           H+ C  Q S VK+SNV Y  I GTS +++AV   CS   PC  I+M D+ L  +      
Sbjct: 322 HKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSV----HR 381

[Close]

BLAST of Spg039520 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 7.6e-64
Identity = 145/372 (38.98%), Postives = 195/372 (52.42%), Query Frame = 0


Query: 22  QISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLD 81
           + S     +V D+GA GDGT +DT+AF  AW+  C S      ++VP  K++LL+  T  
Sbjct: 78  ETSPDTDISVDDFGARGDGT-DDTKAFEKAWKDACSSGS---VLIVPENKNYLLKQITFS 137

Query: 82  GRHCKS---------------------------------RNINI---------------- 141
           G  CKS                                  N+ +                
Sbjct: 138 G-PCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDS 197

Query: 142 -------VVCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDD 201
                  + C   PT       +N+ +S L+ KDS +MH++ ++ + +  +NL + AP+ 
Sbjct: 198 SCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEH 257

Query: 202 SPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHG 261
           SPNTDGIHI  + +I +  S I TGDDCISI  GS  +  +NI CGPGHGISIGSLG   
Sbjct: 258 SPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRN 317

Query: 262 RRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCD- 321
               V  V V       TTNG RIKTWQGGSG A+NI F+ I+    +NPIIIDQ+YCD 
Sbjct: 318 SEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDS 377

Query: 322 HEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDE 337
            + C  Q SAVK+SNV Y  I GTS S++AV F CS + PC+  ++ +I L  +G+ G E
Sbjct: 378 KDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINL--VGNGGKE 437

[Close]

BLAST of Spg039520 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.5e-63
Identity = 129/343 (37.61%), Postives = 191/343 (55.69%), Query Frame = 0


Query: 30  NVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLDGRHCKS-R 89
           +V ++GA GDG  +DTQAF  AW+  C S++     +VP GK++LL+     G  CKS R
Sbjct: 69  SVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYLLKSTRFRG-PCKSLR 128

Query: 90  NINIV------------------------------------------------------- 149
           N  I+                                                       
Sbjct: 129 NFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKSK 188

Query: 150 VCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDDSPNTDGIH 209
            C + PT   L + +N+++  L  K++ Q+ I++E+   + ++N+ I AP DSPNTDGIH
Sbjct: 189 PCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIH 248

Query: 210 IQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHGRRETVEQV 269
           I N+  I +  SDI TGDDCISI DG+  + I ++ CGPGHGISIGSLG    +  V  +
Sbjct: 249 ITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGI 308

Query: 270 HVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCDHEKCKNQTS 317
           +V    F+ + NG RIKT+QGGSG A+NI F+ I      NPIIIDQ YCD +KC++Q S
Sbjct: 309 NVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDKCEDQES 368

[Close]

BLAST of Spg039520 vs. ExPASy TrEMBL
Match: A0A6J1BX76 (probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111006160 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.4e-145
Identity = 271/408 (66.42%), Postives = 304/408 (74.51%), Query Frame = 0


Query: 3   MANFSAFFVLVLAIFVQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKS-SDS 62
           MANF A FVLVLAIF Q  QI +  +FNV++YGAVG+G  EDTQAFL+AW+ATCKS S S
Sbjct: 1   MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS 60

Query: 63  SPAMVVPSGKSFLLQPFTLDGRHCKSRNINIV---------------------------- 122
           SP MVVP GKSFLLQPFT +GR CKS NI IV                            
Sbjct: 61  SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 120

Query: 123 ------------------------------VCGRKPTGFILEHSENVHMSGLTFKDSPQM 182
                                         VCG+KPTGFILEHSE V + GLTFKDSPQM
Sbjct: 121 HMKGLKIQGSGTINGRGSTWWRLSCTQNKRVCGKKPTGFILEHSEKVQIKGLTFKDSPQM 180

Query: 183 HIALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKI 242
           H+A  RS+ +Y  NLHIQAP +SPNTDGIHIQNS +I IR S I TGDDCISIGDGS++I
Sbjct: 181 HMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRI 240

Query: 243 SISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIA 302
           SISNI CGPGHGISIGSLGKHG+RETVE +HV+DVTFTGTTNGARIKTWQGG GYAR+I+
Sbjct: 241 SISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHIS 300

Query: 303 FEGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAI 352
           FEGI SRGSSNPI+IDQFYCDHE+CKNQTSAVKISNVLYR+I GTS S+IAV  ACSD+I
Sbjct: 301 FEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSI 360

[Close]

BLAST of Spg039520 vs. ExPASy TrEMBL
Match: A0A0A0KS58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G635400 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.1e-133
Identity = 252/409 (61.61%), Postives = 289/409 (70.66%), Query Frame = 0


Query: 10  FVLVLAIFVQSLQI-SAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDS--SPAMV 69
           F L+L I +Q LQI S+  +FNVLDYGAVGDG  EDTQAFLDAW+A CKSS+S  SP M+
Sbjct: 4   FFLILIISLQFLQINSSSKTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPIML 63

Query: 70  VPSGKSFLLQPFTLDGRHCKSRNINIV--------------------------------- 129
           VP GKSFLLQPFTLDG  CKS   NI+                                 
Sbjct: 64  VPPGKSFLLQPFTLDGYDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKQCRQWILLKHM 123

Query: 130 ---------------------------VCGRKPTGFILEHSENVHMSGLTFKDSPQMHIA 189
                                      VC R+PT  ILE SE VH+  + FKDSPQMH+A
Sbjct: 124 KGLVVKGSGTINAQGNTWWDLCTNEEKVCTRRPTSLILEDSEKVHIKNIAFKDSPQMHMA 183

Query: 190 LERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISIS 249
           +E SKY+++ NL+I+AP DSPNTDGIHIQ+S KISI  S I TGDDCISIGDGS +I+IS
Sbjct: 184 IENSKYVFVKNLYIEAPGDSPNTDGIHIQHSRKISITSSSIRTGDDCISIGDGSYRINIS 243

Query: 250 NIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEG 309
            I CGPGHGISIGSLGKHG  E VE +HVKDVTFT TTNG RIKTWQGGSGYARNI FEG
Sbjct: 244 KIACGPGHGISIGSLGKHGNHEKVEFIHVKDVTFTDTTNGVRIKTWQGGSGYARNIIFEG 303

Query: 310 IISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCR 354
           I+SRGS NPIIIDQFYCDHEKCKNQTSAVK+SNVLY ++ GTSKSKIAV+ ACS+  PCR
Sbjct: 304 IVSRGSLNPIIIDQFYCDHEKCKNQTSAVKVSNVLYNRVSGTSKSKIAVNLACSETEPCR 363

[Close]

BLAST of Spg039520 vs. ExPASy TrEMBL
Match: A0A1S4E2F6 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103498735 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.4e-129
Identity = 249/412 (60.44%), Postives = 284/412 (68.93%), Query Frame = 0


Query: 10  FVLVLAIFVQSLQISAKIS---FNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDS--SPA 69
           FVL+L   ++ LQI++  S   FNVLDYGAVGDG  EDTQAFLDAW+A CKSS+S  SP 
Sbjct: 4   FVLILITSLRFLQINSSASSQTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPI 63

Query: 70  MVVPSGKSFLLQPFTLDGRHCKSRNINIV------------------------------- 129
           M+VP GKSFLLQPFTLDG  CKS   NI+                               
Sbjct: 64  MLVPGGKSFLLQPFTLDGHDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKECRQWILLK 123

Query: 130 -----------------------------VCGRKPTGFILEHSENVHMSGLTFKDSPQMH 189
                                        VC R+PT  ILE S+ VH+  L FKDSPQMH
Sbjct: 124 HMKGLVVKGSGSINAQGNSWWDLCTNEEKVCTRRPTSLILEDSDKVHIKNLAFKDSPQMH 183

Query: 190 IALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKIS 249
           +A+E SKY+YI NL+I+AP DSPNTDGIHIQ S K+SI +S I TGDDCISIGDGS +I+
Sbjct: 184 MAIESSKYVYIKNLYIEAPGDSPNTDGIHIQRSKKVSISRSSIRTGDDCISIGDGSYRIN 243

Query: 250 ISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAF 309
           IS I CGPGHGISIGSLGKHG  ETVE VHVKDVTFT TTNG RIKTWQGGSGYARNI F
Sbjct: 244 ISKIACGPGHGISIGSLGKHGDHETVEFVHVKDVTFTDTTNGFRIKTWQGGSGYARNIIF 303

Query: 310 EGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIP 354
           EG++SRGS NPIIIDQFYCDHEKCKN TSAVKISNV Y +I GTS+SKIAV+  CS+  P
Sbjct: 304 EGMVSRGSFNPIIIDQFYCDHEKCKNHTSAVKISNVSYNRISGTSRSKIAVNLVCSETKP 363

[Close]

BLAST of Spg039520 vs. ExPASy TrEMBL
Match: A0A2N9H8U8 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS26495 PE=3 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.8e-116
Identity = 224/355 (63.10%), Postives = 266/355 (74.93%), Query Frame = 0


Query: 3   MANFSAFFVLVLAIFVQS------LQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATC 62
           MANF    VLVLA+ V S      L  S K+ +NV+DYGAVGDG  +DTQAF DAW ATC
Sbjct: 1   MANF-LLHVLVLALCVLSNQSHGILTDSVKV-YNVVDYGAVGDGLADDTQAFSDAWNATC 60

Query: 63  KSSDSSPAMVVPSGKSFLLQPFTLDGRHCKSRNINIVVCGRKPTGFILEHSENVHMSGLT 122
           +SS S PAM VP G +FLLQP T +G  CKSRNI +  CG+KPTGF++E+S NVH+ GLT
Sbjct: 61  QSSVSFPAMYVPQGMTFLLQPLTFNG-SCKSRNITVQGCGKKPTGFVIENSNNVHIDGLT 120

Query: 123 FKDSPQMHIALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISI 182
           F DSPQMHIA ERS +++  +L IQAP DS NTDGIHIQ+S  I I    IATGDDCISI
Sbjct: 121 FVDSPQMHIAFERSTWVHATSLTIQAPKDSRNTDGIHIQHSQNIIIDNIRIATGDDCISI 180

Query: 183 GDGSAKISISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGS 242
            DGS+ I+IS I CGPGHGISIGSLG  G+ ETV+ VHVKDV+FTGTTNG RIKTWQGG 
Sbjct: 181 ADGSSHINISKIDCGPGHGISIGSLGAKGKHETVQFVHVKDVSFTGTTNGVRIKTWQGGK 240

Query: 243 GYARNIAFEGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVH 302
           GYAR+I FE I+S GS+ PIIIDQ+YC HE C NQ SAV++S+VLY QI GTS ++IAV 
Sbjct: 241 GYARHITFERIVSNGSARPIIIDQYYCAHEHCTNQKSAVQVSDVLYDQISGTSDAEIAVK 300

Query: 303 FACSDAIPCRNILMKDIELRPIGDDGDEETSSYCGNVVDGRRNGTVIPSVPCLNE 352
           FACS ++PC NI M++I L     +   +TSS C N V G RNG +IP+VPCL+E
Sbjct: 301 FACSKSLPCTNIFMRNITLH--STENGSKTSSICQN-VRGLRNGEIIPNVPCLHE 349

[Close]

BLAST of Spg039520 vs. ExPASy TrEMBL
Match: A0A2N9EX52 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11208 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.3e-110
Identity = 225/413 (54.48%), Postives = 268/413 (64.89%), Query Frame = 0


Query: 3   MANFSAFFVLVLAIFVQS------LQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATC 62
           MANF    VLVLA+ V S      L  S K+ +NV+DYGAVGDG  +DTQAF DAW ATC
Sbjct: 1   MANF-LLHVLVLALCVLSNQSHGILTDSVKV-YNVVDYGAVGDGLADDTQAFSDAWNATC 60

Query: 63  KSSDSSPAMVVPSGKSFLLQPFTLDGRHCKSRNINIVV---------------------- 122
           +SS S PAM VP G +FLLQP T +G  CKSRNI + +                      
Sbjct: 61  QSSVSFPAMYVPQGMTFLLQPLTFNG-SCKSRNITVQIDANIIAPSDPFAWKCPGNECHR 120

Query: 123 ------------------------------------CGRKPTGFILEHSENVHMSGLTFK 182
                                               CG+KPTGF++E+S NVH+ GLTF 
Sbjct: 121 WITFLNFNGLFISGSGTINGQGTNWWGLSCQQNKRGCGKKPTGFVIENSNNVHIDGLTFV 180

Query: 183 DSPQMHIALERSKYIYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGD 242
           DSPQMHIA ERS +++  +L IQAP DSPNTDGIHIQ+S  I I    IATGDDCISI D
Sbjct: 181 DSPQMHIAFERSTWVHATSLTIQAPKDSPNTDGIHIQHSQNIIIDNIRIATGDDCISIAD 240

Query: 243 GSAKISISNIVCGPGHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGY 302
           GS+ I+IS I CGPGHGISIGSLG  G+ ETV+ VHVKDV+FTGTTNG RIKTWQGG GY
Sbjct: 241 GSSHINISKIDCGPGHGISIGSLGAKGKHETVQFVHVKDVSFTGTTNGVRIKTWQGGKGY 300

Query: 303 ARNIAFEGIISRGSSNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFA 352
           AR+I FE I+S GS+ PIIIDQ+YC HE C NQ SAV++S+VLY QI GTS ++IAV FA
Sbjct: 301 ARHITFERIVSNGSARPIIIDQYYCAHEHCTNQKSAVQVSDVLYDQISGTSDAEIAVKFA 360

[Close]

BLAST of Spg039520 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.3e-74
Identity = 155/386 (40.16%), Postives = 210/386 (54.40%), Query Frame = 0


Query: 22  QISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLD 81
           Q S K   NV  + A GDG  +DTQAF+ AW   C + +S   ++VP G+S+L+     D
Sbjct: 60  QYSGKNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNS--VLLVPQGRSYLVNATKFD 119

Query: 82  G---------------------------------------------------------RH 141
           G                                                           
Sbjct: 120 GPCQEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAAS 179

Query: 142 CKSRNINIVVCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPD 201
           CK    N   C   PT   +  S NV++ GLT ++S QMH+ ++RS  + I+ + + +P 
Sbjct: 180 CKKNKSN--PCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPG 239

Query: 202 DSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKH 261
           DSPNTDGIHI  S  + ++ S I+TGDDC+SI +GSAKI +  I CGPGHGISIGSLG+ 
Sbjct: 240 DSPNTDGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQG 299

Query: 262 GRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCD 321
             + TV  V ++      TTNG RIKTWQGG+GY + + FE ++ +  +NPIIIDQFYCD
Sbjct: 300 HSKGTVTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCD 359

Query: 322 H-EKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGD 350
               C+NQTSAV IS ++YR I GT+KS  A++F CSDA+PC +I++ +I L   G+DG 
Sbjct: 360 SPSTCQNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINLE--GNDGK 419

[Close]

BLAST of Spg039520 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 6.0e-72
Identity = 161/395 (40.76%), Postives = 209/395 (52.91%), Query Frame = 0


Query: 5   NFSAFFVLVLAIFVQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPA 64
           NFS FF   L IF  S       + +V  +GAVGDG  +D+QAFL AWEA C S      
Sbjct: 9   NFSLFF---LQIFTSS------NALDVTQFGAVGDGVTDDSQAFLKAWEAVC-SGTGDGQ 68

Query: 65  MVVPSGKSFLLQPFTLDGRHCKSRNINIVVCGR--------------------------- 124
            VVP+G +F+LQP    G  CKS  + + + G+                           
Sbjct: 69  FVVPAGMTFMLQPLKFQG-SCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 128

Query: 125 -------------------KPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANL 184
                              +PT        N+ +SGLT  DSP  HI +    Y+ I++L
Sbjct: 129 EGDGEINGQGSSWWEHKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSL 188

Query: 185 HIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISI 244
            I AP+ SPNTDGI +  S  + I+   IATGDDCI+I  G++ I IS I CGPGHGISI
Sbjct: 189 RINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISI 248

Query: 245 GSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIII 304
           GSLGK G   TVE V V++  F GT NGARIKTWQGGSGYAR I F GI      NPIII
Sbjct: 249 GSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIII 308

Query: 305 DQFY--CDHEKCKN-QTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIEL 350
           DQFY   D +  K+ ++SAV++S V++    GTSKS+  V F CS+ +PC  I ++D+++
Sbjct: 309 DQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKI 368

[Close]

BLAST of Spg039520 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.9e-71
Identity = 162/392 (41.33%), Postives = 212/392 (54.08%), Query Frame = 0


Query: 5   NFSAFFVLVLAIFVQSLQISAKISFNVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPA 64
           NFS FF   L IF  S       + +V  +GAVGDG  +D+QAFL AWEA C S      
Sbjct: 8   NFSLFF---LQIFTSS------NALDVTQFGAVGDGVTDDSQAFLKAWEAVC-SGTGDGQ 67

Query: 65  MVVPSGKSFLLQPFTLDGRHCKSRNINIVVCGR--KPT------------------GFIL 124
            VVP+G +F+LQP    G  CKS  + + + G+   P+                  G ++
Sbjct: 68  FVVPAGMTFMLQPLKFQG-SCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVI 127

Query: 125 E-----------------------HSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQ 184
           E                          N+ +SGLT  DSP  HI +    Y+ I++L I 
Sbjct: 128 EGDGEINGQGSSWWEHKGSRPTFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRIN 187

Query: 185 APDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSL 244
           AP+ SPNTDGI +  S  + I+   IATGDDCI+I  G++ I IS I CGPGHGISIGSL
Sbjct: 188 APESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSL 247

Query: 245 GKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQF 304
           GK G   TVE V V++  F GT NGARIKTWQGGSGYAR I F GI      NPIIIDQF
Sbjct: 248 GKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQF 307

Query: 305 Y--CDHEKCKN-QTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPI 350
           Y   D +  K+ ++SAV++S V++    GTSKS+  V F CS+ +PC  I ++D+++   
Sbjct: 308 YNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETA 367

[Close]

BLAST of Spg039520 vs. TAIR 10
Match: AT5G39910.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-69
Identity = 161/411 (39.17%), Postives = 204/411 (49.64%), Query Frame = 0


Query: 3   MANFSAF--FVLVLAIFVQSLQISAKIS-------FNVLDYGAVGDGTIEDTQAFLDAWE 62
           MANF  F  F+L L +F     + +  S       FNVL+YGA+GDG  +D++AF DAWE
Sbjct: 1   MANFVMFLNFLLSLTVFHTFQSVQSHFSLYNDNNIFNVLNYGAIGDGFSDDSKAFKDAWE 60

Query: 63  ATCKSSDSSPAMVVPSGKSFLLQPFTLDGRHCKSRNI----------------------- 122
            TC    S   M +P G +FLLQP    G  CKS+ I                       
Sbjct: 61  DTCNYIGSESIMEIPEGNTFLLQPIEFHG-PCKSKKIILSISGNLIAPESPYEWKCNKDD 120

Query: 123 ------------------NIVVCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKY 182
                              ++ C ++P G ++ HS NVH+S +  KDSP   ++LE SK+
Sbjct: 121 CHQWIEFAHINGLYIDGHGLMACLKRPRGVVISHSSNVHISNIMVKDSPNFQMSLEDSKW 180

Query: 183 IYIANLHIQAPDDSPNTDGIHIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGP 242
           + +  L I A  DSPNTDGIHIQ S  + +  S+I TGDDCISIGDGS  I+IS I CGP
Sbjct: 181 VIVKQLTITADGDSPNTDGIHIQRSQNVIVYDSNIRTGDDCISIGDGSKYINISRISCGP 240

Query: 243 GHGISIGSLGKHGRRETVEQVHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGS 302
           GHGISIGSLG++G +ETVE V V+D TF  TTNG RIKTW                    
Sbjct: 241 GHGISIGSLGRYGTKETVENVVVRDCTFRETTNGVRIKTW-------------------- 300

Query: 303 SNPIIIDQFYCDHEKCKNQTSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKD 350
                             QT AV+I NV++  I GTS  K  V   CS ++PCR+I M D
Sbjct: 301 ------------------QTKAVEIKNVMFNHIHGTSIKKPFVQLLCSKSVPCRDIFMND 360

[Close]

BLAST of Spg039520 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.7e-69
Identity = 154/380 (40.53%), Postives = 196/380 (51.58%), Query Frame = 0


Query: 30  NVLDYGAVGDGTIEDTQAFLDAWEATCKSSDSSPAMVVPSGKSFLLQPFTLDG------- 89
           +V ++GA G+G  +DT+AF DAW+  C SS     ++VP   + LL+P  L G       
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTAC-SSKVKTRILVPENYTCLLRPIDLSGPCKARLT 113

Query: 90  --------------------------------------------------RHCKSRNINI 149
                                                             R CK  + N 
Sbjct: 114 LQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN- 173

Query: 150 VVCGRKPTGFILEHSENVHMSGLTFKDSPQMHIALERSKYIYIANLHIQAPDDSPNTDGI 209
             C   PT       +N+ +  L   DS QMHIAL   + + I+ L + AP  SPNTDGI
Sbjct: 174 -PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGI 233

Query: 210 HIQNSHKISIRKSDIATGDDCISIGDGSAKISISNIVCGPGHGISIGSLGKHGRRETVEQ 269
           HI  S  I I  + ++TGDDCISI   S +ISISNI+CGPGHGISIGSLGK    E V  
Sbjct: 234 HISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRD 293

Query: 270 VHVKDVTFTGTTNGARIKTWQGGSGYARNIAFEGIISRGSSNPIIIDQFYCDHEK-CKNQ 329
           + V     + T NG RIKTWQGGSG    I F  I     SNPIIIDQ+YCD  K C NQ
Sbjct: 294 ITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQ 353

Query: 330 TSAVKISNVLYRQIWGTSKSKIAVHFACSDAIPCRNILMKDIELRPIGDDGDEETSSYCG 352
           TSA+ I N+ +  + GTS SK A+  +CSD+ PCRNIL++DI+L P   +GD  T S+C 
Sbjct: 354 TSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEP--SNGDGFTESFCW 413

The following BLAST results are available for this feature:

BLAST of Spg039520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133622.1	2.9e-145	66.42	probable polygalacturonase At1g80170 [Momordica charantia]	[more]
XP_038889205.1	4.2e-136	64.42	probable polygalacturonase At1g80170 [Benincasa hispida]	[more]
KGN52445.1	4.4e-133	61.61	hypothetical protein Csa_008476 [Cucumis sativus]	[more]
XP_031741769.1	2.0e-130	62.37	probable polygalacturonase At1g80170, partial [Cucumis sativus]	[more]
XP_016902407.1	5.0e-129	60.44	PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo]	[more]

BLAST of Spg039520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW07	8.4e-71	40.76	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q94AJ5	6.7e-68	40.53	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
O22818	4.5e-64	37.66	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
Q02096	7.6e-64	38.98	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
O23147	6.5e-63	37.61	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]

BLAST of Spg039520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BX76	1.4e-145	66.42	probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A0A0KS58	2.1e-133	61.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G635400 PE=3 SV=1	[more]
A0A1S4E2F6	2.4e-129	60.44	probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103498735 PE=...	[more]
A0A2N9H8U8	1.8e-116	63.10	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS26495 PE=3 SV=1	[more]
A0A2N9EX52	3.3e-110	54.48	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11208 PE=3 SV=1	[more]

BLAST of Spg039520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70500.1	8.3e-74	40.16	Pectin lyase-like superfamily protein	[more]
AT3G15720.1	6.0e-72	40.76	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	3.9e-71	41.33	Pectin lyase-like superfamily protein	[more]
AT5G39910.1	3.6e-69	39.17	Pectin lyase-like superfamily protein	[more]
AT1G80170.1	4.7e-69	40.53	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 130..156 e-value: 42.0 score: 13.0 coord: 210..231 e-value: 210.0 score: 10.7 coord: 180..200 e-value: 3300.0 score: 3.5 coord: 239..260 e-value: 4700.0 score: 2.5 coord: 157..178 e-value: 1200.0 score: 6.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 94..333 e-value: 7.3E-74 score: 248.7
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 187..200
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 19..80 e-value: 1.3E-7 score: 33.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 81..342 e-value: 7.5E-82 score: 277.5
None	No IPR available	PANTHER	PTHR31375:SF41	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 102..343 coord: 25..93
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 102..343 coord: 25..93
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 14..348

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg039520.1	Spg039520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity