Spg039443 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039443
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPectinesterase
Locationscaffold10: 44375649 .. 44379301 (+)
RNA-Seq ExpressionSpg039443
SyntenySpg039443
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGATTCTCAAACAATAATATTTTTCAAAAAGTTAGAGAAAATAAAACTAAACTAAATACAGCATAAAAAGAGTCATGATACAAGGATCTAATTCCAAACCTTAATTTAACCTTTTTCAATAATAAAAAAAAAAAAAAATCCATCGGTTTTTCTTTAATTTTTTTTTTTTTTTAATTGGAAGATTCCGGCTACTTGGTAATCTCCACCGATGCCACCTCAAAGGCTCAACAAAGAAAGCAGGAGAAAAAAAAAAAAAAAAAAATTGGAAAGAATCTGCTGAACCGACGATAGGTAATGGGTTCGATGCCAAATTTGGCATTTTCGCTTTTCGTTCCATTTTATCACCACAATTGTTCTTCATCTTCAACCTGCCTTCCAACAACAGAAAGGCGCGAACTTCGAGAGAGGGTTTCATGGCGGCCCTGCCCAGGGTGATCACCGTCGCACAAGACGGCTGTGCCGATTTCCGCACGGTTCAAGAAGCCATAGACGCGATTCCATTCAGCAATACCTGCCGGACTGTGATCCGTGTGTCTCCTGGCATTTACAGACAGCCCCTCTACGTCCCCAAGACCAAGAATTTCATCACCTTCGCCGGCTTGAGCCCTGAGACTACTGTTCTCACTTGGGACAATACTGCCACTAAAATTACCCACCACCAAGTCCGTTTCTCATGATCATTTTCTCATTTCGTATCTGGGTTTGCGTTTAATTTGCAGAATTATAATGTTCATCTCTGTGTATCTCTCTCTGTCGCTGGTTAGGCTTCTCGTGTCATTGGAACTGGGACTTTTGGTTGCGGGAGTACAATTGTGGAAGGAGAGGATTTCATTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAGGTACCCTTTCCAGGAGCTCTTTCATCATCTTTATCTTTACTTTGGACTTTTATTTGACTAGTGATATGGAGATGAAAATAGGTCCCCTTTCTAGAGTTCATAATTTGAGGTTGATTTTGAAATTGTACAATGTGGAATCTCTGTGCAATCAAATCTGCTTTATTTTTCTGATCAACTTCTTTGGCGTTTTGAAGTTGCTATTGTTTCAGTTATGTGTGGCTTCTTTGTTTTCTACAGGGTTCCGGACAAGCTGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTCTATAATTGCAGGTTTCTCGGCTGGCAGGTATGCCAATCGTCTAATTCAATTGGTTAATTGCTAGTTAGTTCAAGAGGTCAGACATTATTTCTGCAACAACCTACTGGTTTGTGTTGTACTTCAGCACTTTTCTTCTTACCCTTCTTTGAATCTGTATGAAACAACCTTTTACCATAAAAAACTTGTGGGCTGCTTGGGTGGATGTTCAAGACACAATCTTTATGCAAGTGCCCCAGGCTTTTGGTAAGAGGTATTTCTCAAGATAAAGAGTGATATGTTCTATGGAGAGGGTCTCGAGCTAAGTTCAAGAGTGCTAGTTTTAGCTCAGTTGCCTCAACTGCCATCAAGCTTTCTTTGCTTTTGCTATCTATAACACTGTTAGGATCTACAGTTTTATGCTGTTGTCGTTATCGGATAACACTGTTAGGATCTACATTTGATGTTGTTGTCGATTTCGGATAGCTCTATCCTTTGGCTAGTTCAATCAGCCAAGACCATTCACGATCACAGTAGATTGTTTTTCTGTACTTCTATTGCCTGCTGTTGTTTCTTCTGAAATATTTATGGCTATCCGGGAAATAGCTATGAACATTTTCATTTTTCTCATATAATTTGATGCAGGATACTCTGTACCTACATCATGGAAAACAGTATTTGAAGGACTGCTATATTGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCGTTGTCATATCCACTGCAAGTCGCCTGGCTTTATTACAGCACAGAGCAGGAAATCTTCTCAAGAGACAACAGGTTATGTGTTCTTGAGGTGCGTTGGTAGAAATTATGTTCCTCTTGTTCAAAATTTCTGGTGTCTCATAGATTACTGAAACACTGATTTATGTTTGGCTCTATGACGTCGATCATCTCTTTTTGGACAATAACTTTACGGTTAAATTCTTTCATCCTTTTAGTGGTGGTCAATTATCCTTTTAGTGGTGGTCAATTTTAGTAATAGTTCGTAGTTGTGATTATATCATTTACATTTTAGAAGCTAAGGACGAGAACAGTTGTTTTTGCCCTTCTTTAAAATTTTGTTTCTTTATTAGGTTGGAATGCAAAGTGACTTTGGGATTATAATTCAAACTCTATTGATTGCTGCCTAGGTAGGATATTTAAAATCCTGTAAGTTTTCTAACAATCAAATATCGTAAAGTTTGGGTGTACATAAATTGTCTGAGATGCTCATGATATATATAAAAAAGAGAAGTATTCAGGAATATTGGATCTTGTGGTCTCTAATGTATGGTTCCTTGCTGCTGTGAATCTGAATTCAAGATTAGTTTTGAAGCATTGAACATTTAATTTGGACCAGAAACCTAGTTTCTTAACCCTCGCCCCCCACGTTATCCTCCTCGGCTTCCTATTTGTCATTTATTAATTATTGGTCAGCTTAATATTTGGTTACAATACATGTCTTCTTTCTGCACCCGTACCTGCTGGAAGATATGCTTTTTGTGAGTGATTGCAATCTGCCATATTGGTTAGAGTTTCAAAGAAAGTCAATACTTTATCGTTATTGTCTTTTAAATCGTTTACTGTTTCACACTGAAAGGCTTCTGGTGATATCTTAAAATTTTGACTCTAGTATTCTTGATTCTGTTCACTACTCGTAATGTGTCATATTCAACTGATTCCGACTGTTTTCTGCAAGTTTCCTTGTCTGACATGCTTCTTCATATCGTTTTTCTGTTTTCTATAACGATTTTTTCCCCATTATATGTAGGTGTGTGATCACAGGCAGTGGCGGGACTTCGTATAGTCATCTCGGACGGCCATGGGGACCGTTTGCAAGGGTGGTCTTTGCCTACACATACATGGATGCATGTGTCAAACCAGGAGGCTGGGACAACTGGGGAAAAGCTGAAAATGAGAGAACTGCTTGTTTTTACGAGTACAAGTATGTCGTGTTTAATGCTTGTCGCCTTATTGTTACACAATATCATGCTTATTACAACTTTGAACGACAATTATATGATGAATGGGAACATCGTCGAATGCCTAGATCACCTGATAGGAGACTCATTTTTTTGGTACAGGTGTTTTGGGCCAGGGAGTTGCTCGATGAAAAGGGTATGTTGGGCGAGAGAATTAGTAGATGAAGAAGCTGACGAGTTCCTTCTGCATCGATTCATCGATCCCGACATCGAAAGGCCTTGGCTATGCCAGAGGATGGCATTGAGGATTCCCTTTTCTGCTTAAGAATGGCATGCTAATGATTTCAAAACAAAAATCAGCAAAAATCTCAAGGTTTGGCAGTAATTTATGAGTAGAAAGAGTCTCTAAATGTTGAATTTTTGTTGCCTGCTGGTGCGTTAGAGGATAAAATAAAAACAAAACAAAAAAACAAACTAATCCTATATGGTGGCACACGGCTTTTTCTGGACTGATATTGTGAATCTAAGTAATTTGACGAATTTTTCAAATTACTGTATTTATCTTTGGTATTTTTTTTC

mRNA sequence

ATGGCGGCCCTGCCCAGGGTGATCACCGTCGCACAAGACGGCTGTGCCGATTTCCGCACGGTTCAAGAAGCCATAGACGCGATTCCATTCAGCAATACCTGCCGGACTGTGATCCGTGTGTCTCCTGGCATTTACAGACAGCCCCTCTACGTCCCCAAGACCAAGAATTTCATCACCTTCGCCGGCTTGAGCCCTGAGACTACTGTTCTCACTTGGGACAATACTGCCACTAAAATTACCCACCACCAAGCTTCTCGTGTCATTGGAACTGGGACTTTTGGTTGCGGGAGTACAATTGTGGAAGGAGAGGATTTCATTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAGGGTTCCGGACAAGCTGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTCTATAATTGCAGGTTTCTCGGCTGGCAGGATACTCTGTACCTACATCATGGAAAACAGTATTTGAAGGACTGCTATATTGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCGTTGTCATATCCACTGCAAGTCGCCTGGCTTTATTACAGCACAGAGCAGGAAATCTTCTCAAGAGACAACAGGTTATGTGTTCTTGAGGTGTGTGATCACAGGCAGTGGCGGGACTTCGTATAGTCATCTCGGACGGCCATGGGGACCGTTTGCAAGGGTGGTCTTTGCCTACACATACATGGATGCATGTGTCAAACCAGGAGGCTGGGACAACTGGGGAAAAGCTGAAAATGAGAGAACTGCTTGTTTTTACGAGTACAAGTGTTTTGGGCCAGGGAGTTGCTCGATGAAAAGGGTATGTTGGGCGAGAGAATTAGTAGATGAAGAAGCTGACGAGTTCCTTCTGCATCGATTCATCGATCCCGACATCGAAAGGCCTTGGCTATGCCAGAGGATGGCATTGAGGATTCCCTTTTCTGCTTAA

Coding sequence (CDS)

ATGGCGGCCCTGCCCAGGGTGATCACCGTCGCACAAGACGGCTGTGCCGATTTCCGCACGGTTCAAGAAGCCATAGACGCGATTCCATTCAGCAATACCTGCCGGACTGTGATCCGTGTGTCTCCTGGCATTTACAGACAGCCCCTCTACGTCCCCAAGACCAAGAATTTCATCACCTTCGCCGGCTTGAGCCCTGAGACTACTGTTCTCACTTGGGACAATACTGCCACTAAAATTACCCACCACCAAGCTTCTCGTGTCATTGGAACTGGGACTTTTGGTTGCGGGAGTACAATTGTGGAAGGAGAGGATTTCATTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAGGGTTCCGGACAAGCTGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTCTATAATTGCAGGTTTCTCGGCTGGCAGGATACTCTGTACCTACATCATGGAAAACAGTATTTGAAGGACTGCTATATTGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCGTTGTCATATCCACTGCAAGTCGCCTGGCTTTATTACAGCACAGAGCAGGAAATCTTCTCAAGAGACAACAGGTTATGTGTTCTTGAGGTGTGTGATCACAGGCAGTGGCGGGACTTCGTATAGTCATCTCGGACGGCCATGGGGACCGTTTGCAAGGGTGGTCTTTGCCTACACATACATGGATGCATGTGTCAAACCAGGAGGCTGGGACAACTGGGGAAAAGCTGAAAATGAGAGAACTGCTTGTTTTTACGAGTACAAGTGTTTTGGGCCAGGGAGTTGCTCGATGAAAAGGGTATGTTGGGCGAGAGAATTAGTAGATGAAGAAGCTGACGAGTTCCTTCTGCATCGATTCATCGATCCCGACATCGAAAGGCCTTGGCTATGCCAGAGGATGGCATTGAGGATTCCCTTTTCTGCTTAA

Protein sequence

MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPDIERPWLCQRMALRIPFSA
Homology
BLAST of Spg039443 vs. NCBI nr
Match: XP_023551220.1 (pectinesterase 31 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551221.1 pectinesterase 31 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.8 bits (1688), Expect = 3.7e-184
Identity = 300/318 (94.34%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAQDGCADFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAASPRVLTVAQDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETTVLTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCY+EGSVDFIFGNSTALLERCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYVEGSVDFIFGNSTALLERCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           IHCKS GFITAQSRKSSQETTGYVFLRCVITGS GTSY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSCSM RVCWARELVDEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Spg039443 vs. NCBI nr
Match: KAG6578565.1 (Pectinesterase 31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 653.3 bits (1684), Expect = 1.1e-183
Identity = 300/318 (94.34%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAQDGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETTVLTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLERCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           IHCKS GFITAQSRKSSQETTGYVFLRCVITGS GTSY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSCSM RVCWARELVDEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Spg039443 vs. NCBI nr
Match: XP_022938497.1 (pectinesterase 31 [Cucurbita moschata])

HSP 1 Score: 652.9 bits (1683), Expect = 1.4e-183
Identity = 299/318 (94.03%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAQDGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETTVLTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLERCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGS GTSY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSCSM RVCWARELVDEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Spg039443 vs. NCBI nr
Match: XP_022993645.1 (pectinesterase 31 [Cucurbita maxima])

HSP 1 Score: 649.8 bits (1675), Expect = 1.2e-182
Identity = 298/318 (93.71%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAQDGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETTVLTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLERCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           IHCKS GFITAQSRKSSQETTGYVFLRCVITGS G+SY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGSSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSC M RVCWARELVDEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCPMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Spg039443 vs. NCBI nr
Match: XP_022134347.1 (pectinesterase 31 [Momordica charantia])

HSP 1 Score: 647.9 bits (1670), Expect = 4.6e-182
Identity = 296/318 (93.08%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRVITVA DGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVITVAHDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLSPE+TVLTWDNTATKI HHQASR+IGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLSPESTVLTWDNTATKINHHQASRIIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNC+FLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCKFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           +HCKSPGFITAQSRKSSQETTGYVFLRCVITGSGG SY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGNSYIHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSC MKRVCWARELVDEE DEFLLH+FIDP+
Sbjct: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSCQMKRVCWARELVDEETDEFLLHQFIDPN 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLCQRMA RIPFSA
Sbjct: 301 VERPWLCQRMASRIPFSA 318

BLAST of Spg039443 vs. ExPASy Swiss-Prot
Match: Q9LVQ0 (Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.1e-157
Identity = 249/316 (78.80%), Postives = 283/316 (89.56%), Query Frame = 0

Query: 3   ALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAG 62
           A  R++ V+QDG  D+ +VQ+AID++P  NTCRTVIR+SPGIYRQP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQ 122
           +SPE TVLTW+NTA+KI HHQASRVIGTGTFGCGS IVEGEDFIAENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIH 182
           AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLE CHIH
Sbjct: 122 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIH 181

Query: 183 CKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACV 242
           CKS GFITAQSRKSSQE+TGYVFLRCVITG+G + Y +LGRPWGPF RVV AYTYMDAC+
Sbjct: 182 CKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACI 241

Query: 243 KPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPDIE 302
           +  GW NWG AENER+ACFYEY+CFGPGSCS +RV W+REL+D+EA  F+ H F+DP+ +
Sbjct: 242 RNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQD 301

Query: 303 RPWLCQRMALRIPFSA 319
           RPWLC RM ++ P+SA
Sbjct: 302 RPWLCLRMGVKTPYSA 317

BLAST of Spg039443 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.1e-72
Identity = 140/289 (48.44%), Postives = 178/289 (61.59%), Query Frame = 0

Query: 17  DFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSPETTVLTWDNTA 76
           DF  +Q+AID++P  N  R VI+V  G+Y++ + +P  K FIT  G   E T + W +TA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 77  TKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSGQAVAIRVTAD 136
                   S+    GT+   S  V    F+A+NITF N++P         QAVA+RV+AD
Sbjct: 155 ----QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 214

Query: 137 RCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIHCKSP--GFI 196
             AF+ CR LG QDTLY H G+ Y KDCYIEGSVDFIFGN+ +L E CH+H  +   G +
Sbjct: 215 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 274

Query: 197 TAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACVKPGGWDN 256
           TAQ R S  E TG+ F++C +TG+G     +LGR WGPF+RVVFAYTYMD  + P GW N
Sbjct: 275 TAQGRSSVLEDTGFSFVKCKVTGTG---VLYLGRAWGPFSRVVFAYTYMDNIILPRGWYN 334

Query: 257 WGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFID 299
           WG    E T  + +YKC G G+    RV WAREL DEEA  FL   FID
Sbjct: 335 WGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFID 376

BLAST of Spg039443 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.8e-62
Identity = 125/295 (42.37%), Postives = 172/295 (58.31%), Query Frame = 0

Query: 7   VITVAQDGCADFRTVQEAIDAIP--FSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLS 66
           +I V Q G  DF  +QEAI++IP   +N+    I V PGIYR+ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIHCK 186
           A+RV AD+ AFY C    +QDTL   +G  Y K+CYIEG+ DFI G++++L ERCH+H  
Sbjct: 170 ALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSL 229

Query: 187 SP--GFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACV 246
           SP  G ITAQ R S+ E +G+ FL C +TGSG T    LGRPWG ++RVVFAY++    V
Sbjct: 230 SPNNGSITAQMRTSATEKSGFTFLGCKLTGSGST---FLGRPWGAYSRVVFAYSFFSNVV 289

Query: 247 KPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFI 298
            P GW+ WG +  E T  + EYKC+GPG+   +RV W+++L DEEA  FL   FI
Sbjct: 290 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFI 326

BLAST of Spg039443 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.2e-61
Identity = 126/318 (39.62%), Postives = 181/318 (56.92%), Query Frame = 0

Query: 6   RVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSP 65
           +VITV+ +G A FR+VQ+A+D+IP +N     I+++PG YR+ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTVLTWDNTATKITHHQASRVIGTG----TFGCGSTIVEGEDFIAENITFENSSP---- 125
           + T + W        H +AS +   G    T+   S  V    F A NI+F N++P    
Sbjct: 119 DVTAIEW--------HDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLP 178

Query: 126 -QGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALL 185
                QAVA R++ D+  F  C F G QDTL    G+ Y K+CYIEGS+DFIFGN  ++ 
Sbjct: 179 GMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMY 238

Query: 186 ERCHIHCKSP--GFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFA 245
           + C +H  +   G I A  R   +E TG+ F+ C +TG+G     ++GR  G ++R+V+A
Sbjct: 239 KDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPL---YVGRAMGQYSRIVYA 298

Query: 246 YTYMDACVKPGGWDNWG-KAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLL 305
           YTY DA V  GGWD+W  K+   +TA F  Y C+GPG+ + + V WAR L  E A  F+ 
Sbjct: 299 YTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIA 358

Query: 306 HRFIDPDIERPWLCQRMA 312
             F++    R W+  R A
Sbjct: 359 KSFVN---GRHWIAPRDA 362

BLAST of Spg039443 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.6e-58
Identity = 119/306 (38.89%), Postives = 173/306 (56.54%), Query Frame = 0

Query: 7   VITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSPE 66
           V+TV   GC +F  VQ AID +P  ++ +T+I V+ G YR+ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSG 126
            T + W++TA               T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKS----------AGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHI 186
           QAVA+R+  D+ AFY C F G QDTL    G+ + K+C+I+GS+DFIFGN  +L + C I
Sbjct: 210 QAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTI 269

Query: 187 HCKS-------PGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFA 246
           +  +        G ITAQ R+S  E +G+ F+ C I GSG      LGR WG +A VVF+
Sbjct: 270 NSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEIL---LGRAWGAYATVVFS 329

Query: 247 YTYMDACVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLH 301
            TYM   + P GW+NWG +  E+T  F E+KC+GPG+   +RV + ++L D EA  F+  
Sbjct: 330 NTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDV 382

BLAST of Spg039443 vs. ExPASy TrEMBL
Match: A0A6J1FE78 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 6.9e-184
Identity = 299/318 (94.03%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAQDGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETTVLTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLERCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGS GTSY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSCSM RVCWARELVDEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Spg039443 vs. ExPASy TrEMBL
Match: A0A6J1JZ39 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 5.8e-183
Identity = 298/318 (93.71%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAQDGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETTVLTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLERCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           IHCKS GFITAQSRKSSQETTGYVFLRCVITGS G+SY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGSSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSC M RVCWARELVDEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCPMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Spg039443 vs. ExPASy TrEMBL
Match: A0A6J1BZD5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.2e-182
Identity = 296/318 (93.08%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRVITVA DGC DFRTVQEAIDA+PFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVITVAHDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLSPE+TVLTWDNTATKI HHQASR+IGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLSPESTVLTWDNTATKINHHQASRIIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNC+FLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCKFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           +HCKSPGFITAQSRKSSQETTGYVFLRCVITGSGG SY HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGNSYIHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGK ENERTACFYEYKCFGPGSC MKRVCWARELVDEE DEFLLH+FIDP+
Sbjct: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSCQMKRVCWARELVDEETDEFLLHQFIDPN 300

Query: 301 IERPWLCQRMALRIPFSA 319
           +ERPWLCQRMA RIPFSA
Sbjct: 301 VERPWLCQRMASRIPFSA 318

BLAST of Spg039443 vs. ExPASy TrEMBL
Match: A0A5A7TBP8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002680 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.4e-181
Identity = 293/318 (92.14%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVA DGCADFRTVQEAIDA+PFSNTCRTVIRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETT+LTWDNTAT+I HHQA+RVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATEIKHHQAARVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGSG TSY HLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWAREL+DEEADEF+LHRFIDPD
Sbjct: 241 CIKPTGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           ++RPWLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

BLAST of Spg039443 vs. ExPASy TrEMBL
Match: A0A0A0KVN5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.1e-181
Identity = 291/318 (91.51%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MAALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVA DGCADF TVQEAIDA+PFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PETT+LTWDNTATKI HHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 IHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDA 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGSG TSY HLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPD 300
           C+KP GWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWAREL+DEEADEF+LHRFIDPD
Sbjct: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 IERPWLCQRMALRIPFSA 319
           ++RPWLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

BLAST of Spg039443 vs. TAIR 10
Match: AT3G29090.1 (pectin methylesterase 31 )

HSP 1 Score: 557.4 bits (1435), Expect = 7.6e-159
Identity = 249/316 (78.80%), Postives = 283/316 (89.56%), Query Frame = 0

Query: 3   ALPRVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAG 62
           A  R++ V+QDG  D+ +VQ+AID++P  NTCRTVIR+SPGIYRQP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LSPETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQ 122
           +SPE TVLTW+NTA+KI HHQASRVIGTGTFGCGS IVEGEDFIAENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIH 182
           AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLE CHIH
Sbjct: 122 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIH 181

Query: 183 CKSPGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACV 242
           CKS GFITAQSRKSSQE+TGYVFLRCVITG+G + Y +LGRPWGPF RVV AYTYMDAC+
Sbjct: 182 CKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACI 241

Query: 243 KPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFIDPDIE 302
           +  GW NWG AENER+ACFYEY+CFGPGSCS +RV W+REL+D+EA  F+ H F+DP+ +
Sbjct: 242 RNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQD 301

Query: 303 RPWLCQRMALRIPFSA 319
           RPWLC RM ++ P+SA
Sbjct: 302 RPWLCLRMGVKTPYSA 317

BLAST of Spg039443 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.2e-73
Identity = 140/289 (48.44%), Postives = 178/289 (61.59%), Query Frame = 0

Query: 17  DFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSPETTVLTWDNTA 76
           DF  +Q+AID++P  N  R VI+V  G+Y++ + +P  K FIT  G   E T + W +TA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 77  TKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSGQAVAIRVTAD 136
                   S+    GT+   S  V    F+A+NITF N++P         QAVA+RV+AD
Sbjct: 155 ----QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 214

Query: 137 RCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIHCKSP--GFI 196
             AF+ CR LG QDTLY H G+ Y KDCYIEGSVDFIFGN+ +L E CH+H  +   G +
Sbjct: 215 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 274

Query: 197 TAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACVKPGGWDN 256
           TAQ R S  E TG+ F++C +TG+G     +LGR WGPF+RVVFAYTYMD  + P GW N
Sbjct: 275 TAQGRSSVLEDTGFSFVKCKVTGTG---VLYLGRAWGPFSRVVFAYTYMDNIILPRGWYN 334

Query: 257 WGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFID 299
           WG    E T  + +YKC G G+    RV WAREL DEEA  FL   FID
Sbjct: 335 WGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFID 376

BLAST of Spg039443 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 240.0 bits (611), Expect = 2.7e-63
Identity = 125/295 (42.37%), Postives = 172/295 (58.31%), Query Frame = 0

Query: 7   VITVAQDGCADFRTVQEAIDAIP--FSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLS 66
           +I V Q G  DF  +QEAI++IP   +N+    I V PGIYR+ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHIHCK 186
           A+RV AD+ AFY C    +QDTL   +G  Y K+CYIEG+ DFI G++++L ERCH+H  
Sbjct: 170 ALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSL 229

Query: 187 SP--GFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFAYTYMDACV 246
           SP  G ITAQ R S+ E +G+ FL C +TGSG T    LGRPWG ++RVVFAY++    V
Sbjct: 230 SPNNGSITAQMRTSATEKSGFTFLGCKLTGSGST---FLGRPWGAYSRVVFAYSFFSNVV 289

Query: 247 KPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLHRFI 298
            P GW+ WG +  E T  + EYKC+GPG+   +RV W+++L DEEA  FL   FI
Sbjct: 290 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFI 326

BLAST of Spg039443 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.0e-62
Identity = 126/318 (39.62%), Postives = 181/318 (56.92%), Query Frame = 0

Query: 6   RVITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSP 65
           +VITV+ +G A FR+VQ+A+D+IP +N     I+++PG YR+ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTVLTWDNTATKITHHQASRVIGTG----TFGCGSTIVEGEDFIAENITFENSSP---- 125
           + T + W        H +AS +   G    T+   S  V    F A NI+F N++P    
Sbjct: 119 DVTAIEW--------HDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLP 178

Query: 126 -QGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALL 185
                QAVA R++ D+  F  C F G QDTL    G+ Y K+CYIEGS+DFIFGN  ++ 
Sbjct: 179 GMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMY 238

Query: 186 ERCHIHCKSP--GFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFA 245
           + C +H  +   G I A  R   +E TG+ F+ C +TG+G     ++GR  G ++R+V+A
Sbjct: 239 KDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPL---YVGRAMGQYSRIVYA 298

Query: 246 YTYMDACVKPGGWDNWG-KAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLL 305
           YTY DA V  GGWD+W  K+   +TA F  Y C+GPG+ + + V WAR L  E A  F+ 
Sbjct: 299 YTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIA 358

Query: 306 HRFIDPDIERPWLCQRMA 312
             F++    R W+  R A
Sbjct: 359 KSFVN---GRHWIAPRDA 362

BLAST of Spg039443 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 4.0e-59
Identity = 119/306 (38.89%), Postives = 173/306 (56.54%), Query Frame = 0

Query: 7   VITVAQDGCADFRTVQEAIDAIPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLSPE 66
           V+TV   GC +F  VQ AID +P  ++ +T+I V+ G YR+ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTVLTWDNTATKITHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSG 126
            T + W++TA               T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKS----------AGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLERCHI 186
           QAVA+R+  D+ AFY C F G QDTL    G+ + K+C+I+GS+DFIFGN  +L + C I
Sbjct: 210 QAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTI 269

Query: 187 HCKS-------PGFITAQSRKSSQETTGYVFLRCVITGSGGTSYSHLGRPWGPFARVVFA 246
           +  +        G ITAQ R+S  E +G+ F+ C I GSG      LGR WG +A VVF+
Sbjct: 270 NSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEIL---LGRAWGAYATVVFS 329

Query: 247 YTYMDACVKPGGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFLLH 301
            TYM   + P GW+NWG +  E+T  F E+KC+GPG+   +RV + ++L D EA  F+  
Sbjct: 330 NTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDV 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023551220.13.7e-18494.34pectinesterase 31 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551221.1 pectin... [more]
KAG6578565.11.1e-18394.34Pectinesterase 31, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022938497.11.4e-18394.03pectinesterase 31 [Cucurbita moschata][more]
XP_022993645.11.2e-18293.71pectinesterase 31 [Cucurbita maxima][more]
XP_022134347.14.6e-18293.08pectinesterase 31 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LVQ01.1e-15778.80Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1[more]
Q8VYZ33.1e-7248.44Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9SIJ93.8e-6242.37Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Q8LPF34.2e-6139.62Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9ZQA35.6e-5838.89Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FE786.9e-18494.03Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1[more]
A0A6J1JZ395.8e-18393.71Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1[more]
A0A6J1BZD52.2e-18293.08Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1[more]
A0A5A7TBP82.4e-18192.14Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00268... [more]
A0A0A0KVN57.1e-18191.51Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G29090.17.6e-15978.80pectin methylesterase 31 [more]
AT5G19730.12.2e-7348.44Pectin lyase-like superfamily protein [more]
AT2G21610.12.7e-6342.37pectinesterase 11 [more]
AT5G47500.13.0e-6239.62Pectin lyase-like superfamily protein [more]
AT2G36710.14.0e-5938.89Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 8..298
e-value: 6.6E-73
score: 245.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 2..310
e-value: 7.2E-108
score: 362.7
NoneNo IPR availablePANTHERPTHR31321:SF71PECTINESTERASEcoord: 6..317
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 6..317
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 161..170
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 3..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039443.1Spg039443.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0050829 defense response to Gram-negative bacterium
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity