Spg039267 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039267
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCytochrome P450
Locationscaffold10: 45360102 .. 45363116 (+)
RNA-Seq ExpressionSpg039267
SyntenySpg039267
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTCACGTTTGCACTCGTTCTTACAACCCTGGCCTTGGCTGCGACGTCGTTTTTCATCTTCTCTTTGTTATACGACACCCTTTCCTCCTACTGCCTCACTCCTAGACGCATCAAAAACAAGATGGCCAAGCAAGGTGTGCGTGGCCCCAAACCCCGCCCTCTCCTCGGCAACATCCTCGACGTCGCCGCCCTCCTCTCCAAATCCACCTCCTCCGACATGCCCTCTATCTCCCACGACCTCGTCCCCCGCCTCCTCCCCCACTTCCTCGCTTGGTCCGCCCAATTCGGTACGTCCCATATCACCAAATATACCATAACCTATCAACTCAAAACACCCACCGACAAAATTCTAAAAAAGAAAAAATCTAAATCGTACTTTAGACATTCATACTGCGACAGTCACATTCAATAAATTTTTTTTTTTTCCTTTGTAGGTAAGAGATTTATCTTTTGGAATGGGATCGAGCCGAGAATGTGTCTGACCGAAACGGATTTGATCAAAGAGCTTTTGTCGAAATACAGCGCGGTTTCGGGCAAGTCGTGGCTTCAACAACAGGGCTCTAAGCACTTCATTGGCCGAGGTCTCTTAATGGCCAACGGCCAAAACTGGTTGCACCAACGCCATATCGTCGCGCCGGCTTTTGTCGGCGACCGACTCAAGGTTGGTTAAGAATATGTATAAAAGATTAAATTTATCGTACTGCTGGGTTATTTTCTTTTTTCTTCTTTTTGTTGTTTTTCGTTTGAATTTTAGTGTTTTTTTTTTTTTTTTTTTTTTAGAGTTATGCGGGGTACATGGTGGAGTGCACAAGAGAGATGCTTCAATCAATAGAAAATGAGGTAAAATCGGGGCGATCCGAGTTTGAAATCGGCGAGTATATGACCAAACTCACTGCTGACATAATTTCCAGAACCGAGTTTGAAAGCAGTTATGAAAAGGGAAAACAAATCTTTCATTTGCTTACTGTTCTACAGCATCTCTGTGCCCAGGCCAGCCGCCATCTCTGCCTTCCCGGTAGCCGGTAACCTTTTTTTATTTCTTTTCCTTTCCTGCATGCAAATGGAGAAAAAAAAAATACTTCATCCCAATAAAATTTCTAGTATTACGAAAATATCTATTCTCTCGTATCTTTATAAATAATTCTTTAATATTGACGTTGACCTGCATCAATTTTAAAATGATGTTGATGTGACAAACAGTGAATTTTCTCTGATATCAAAAATGTTTTCTCTCTCGTCTTTTCTTTCTCTTAAAAAATGTAGGAAATTCCTGTACAACATTACAGGAATCCAAACACAAAACATCTTATCTGCCATCCGATAACGGATTATCTAAGTTTAATTGTTTCTAATGGTTTTTTTTTATTTTTAATTTTTTTGCAGATTTTTTCCAAGTAAATATAACAGAGAGATAAAGGCATTGAAGGGGAAAGTGGAGGGATTGTTAATGGAGATAATACAAAGCAGAAGGGACTGTGTGGAGATTGGGAGGAGCAGTTCATATGGAAATGATTTATTGGGAATGTTGCTGAATGAGATGCAGAAGAAAAAAGATGGGAATGGTTTGAGTTTGAATTTGCAAATTATTATGGATGAATGCAAGACCTTCTTCTTTGCTGGCCATGAAACAACTGCTCTTTTGCTCACTTGGACTGTAATGTTATTAGCCACTAACCCTTCTTGGCAACAAAAGGTTCGAGCTGAAGTTATGACCGTTTGCAATGGCCAAACTCCCTCGCTCGATCATCTCTCCAAACTCTCCCTGGTACTTTCTCTCTTTCAAAAATGTTACAAATGTCGATAGAAATATCTACATCTCAATTTTATAAAAACGTATCAAAAACTAAAGAAAAAAAATTCTTTATTTTTTATATTGGTACAATGAAAATATCTATTGGTATGTCGATATGATATTTATAAAGATTAACCATGGTTGATGTGAATTCAATATCCTAAAGAGAAAAGGAAGATAACCCACTAACTTTATGATAAAACCCAGTTTCAGAAAGGCACAGAAACTGTCTTTTCAGCTCAAATAGAAGACTTTGGAAAAAGATTTAATTATAATTTACAATCTATAATACAAAACTAAGCAAATGATTTTCAACCCAAAAAAAAAAAAAACTAAATAAATAACTCAAGGAAAAGGGGTGTTGGTGGGACCCAAGTCCATTCCAATATGGCTTACTTCATGGCACACCACTTATAAATGTCTTTTCCCCAACTGAATTAATTCTCACAAATTTTAGAAGGATAAATTTATTTATATATTTTTTTAAAAAAAAAAAAAACCCAACATTCCACTTCCAAAGCTAATGACTGTTCTAGCCACTTAGTGAAAGGACTTTAATTTAATCCACAATTAAGCACATGATCAATCTTTAATTACCATTTATTTTAGAATAATACTTGACTGCATATCGACGAGAGTAAATACTTTTCTACACACTGTTATTAAAAGGTTGACAATTAGAAAAGATTTATTTAGAAAAAAAAATCTCGTTTTCTACCCTTTTATTATGCATTCAAGTATATTTTTCTTCATTTTCTAGAATTTGAGAGTTGAAAATTCTTTGATCATTTACAGTTGAGTATGGTGATAAATGAATCATTGCGACTATATCCACCGGCAAGTATTCTTCCAAGAATGGCATTTGAGGATATAAAATTGGGAGATCTTGAGATTCCGAAAGGATTGTCGATATGGATTCCAGTATTGGCCATTCACCACAGTGAAGAGTTATGGGGAAAAGATGCGAATGAGTTCAACCCAGAACGATTTAGCAATTCGAAAGCCTTTACGTCGGGGAGATTCATCCCTTTTGCTTCGGGCCCTCGAAACTGCGTCGGCCAATCGTTTGCTCTCATGGAAACCAAGATCATCTTGGCTATGCTCATCTCCAAGTTTAGCTTTACAATTTCGGACAATTATCGCCATGCACCTGTGGTTGTGCTTACTATAAAACCTAAATATGGCGTTCAAGTTTGCTTGAAGCCTTTGAATGAATAG

mRNA sequence

ATGGCCGTCACGTTTGCACTCGTTCTTACAACCCTGGCCTTGGCTGCGACGTCGTTTTTCATCTTCTCTTTGTTATACGACACCCTTTCCTCCTACTGCCTCACTCCTAGACGCATCAAAAACAAGATGGCCAAGCAAGGTGTGCGTGGCCCCAAACCCCGCCCTCTCCTCGGCAACATCCTCGACGTCGCCGCCCTCCTCTCCAAATCCACCTCCTCCGACATGCCCTCTATCTCCCACGACCTCGTCCCCCGCCTCCTCCCCCACTTCCTCGCTTGGTCCGCCCAATTCGGTAAGAGATTTATCTTTTGGAATGGGATCGAGCCGAGAATGTGTCTGACCGAAACGGATTTGATCAAAGAGCTTTTGTCGAAATACAGCGCGGTTTCGGGCAAGTCGTGGCTTCAACAACAGGGCTCTAAGCACTTCATTGGCCGAGGTCTCTTAATGGCCAACGGCCAAAACTGGTTGCACCAACGCCATATCGTCGCGCCGGCTTTTGTCGGCGACCGACTCAAGAGTTATGCGGGGTACATGGTGGAGTGCACAAGAGAGATGCTTCAATCAATAGAAAATGAGGTAAAATCGGGGCGATCCGAGTTTGAAATCGGCGAGTATATGACCAAACTCACTGCTGACATAATTTCCAGAACCGAGTTTGAAAGCAGTTATGAAAAGGGAAAACAAATCTTTCATTTGCTTACTGTTCTACAGCATCTCTGTGCCCAGGCCAGCCGCCATCTCTGCCTTCCCGGTAGCCGATTTTTTCCAAGTAAATATAACAGAGAGATAAAGGCATTGAAGGGGAAAGTGGAGGGATTGTTAATGGAGATAATACAAAGCAGAAGGGACTGTGTGGAGATTGGGAGGAGCAGTTCATATGGAAATGATTTATTGGGAATGTTGCTGAATGAGATGCAGAAGAAAAAAGATGGGAATGGTTTGAGTTTGAATTTGCAAATTATTATGGATGAATGCAAGACCTTCTTCTTTGCTGGCCATGAAACAACTGCTCTTTTGCTCACTTGGACTGTAATGTTATTAGCCACTAACCCTTCTTGGCAACAAAAGGTTCGAGCTGAAGTTATGACCGTTTGCAATGGCCAAACTCCCTCGCTCGATCATCTCTCCAAACTCTCCCTGTTGAGTATGGTGATAAATGAATCATTGCGACTATATCCACCGGCAAGTATTCTTCCAAGAATGGCATTTGAGGATATAAAATTGGGAGATCTTGAGATTCCGAAAGGATTGTCGATATGGATTCCAGTATTGGCCATTCACCACAGTGAAGAGTTATGGGGAAAAGATGCGAATGAGTTCAACCCAGAACGATTTAGCAATTCGAAAGCCTTTACGTCGGGGAGATTCATCCCTTTTGCTTCGGGCCCTCGAAACTGCGTCGGCCAATCGTTTGCTCTCATGGAAACCAAGATCATCTTGGCTATGCTCATCTCCAAGTTTAGCTTTACAATTTCGGACAATTATCGCCATGCACCTGTGGTTGTGCTTACTATAAAACCTAAATATGGCGTTCAAGTTTGCTTGAAGCCTTTGAATGAATAG

Coding sequence (CDS)

ATGGCCGTCACGTTTGCACTCGTTCTTACAACCCTGGCCTTGGCTGCGACGTCGTTTTTCATCTTCTCTTTGTTATACGACACCCTTTCCTCCTACTGCCTCACTCCTAGACGCATCAAAAACAAGATGGCCAAGCAAGGTGTGCGTGGCCCCAAACCCCGCCCTCTCCTCGGCAACATCCTCGACGTCGCCGCCCTCCTCTCCAAATCCACCTCCTCCGACATGCCCTCTATCTCCCACGACCTCGTCCCCCGCCTCCTCCCCCACTTCCTCGCTTGGTCCGCCCAATTCGGTAAGAGATTTATCTTTTGGAATGGGATCGAGCCGAGAATGTGTCTGACCGAAACGGATTTGATCAAAGAGCTTTTGTCGAAATACAGCGCGGTTTCGGGCAAGTCGTGGCTTCAACAACAGGGCTCTAAGCACTTCATTGGCCGAGGTCTCTTAATGGCCAACGGCCAAAACTGGTTGCACCAACGCCATATCGTCGCGCCGGCTTTTGTCGGCGACCGACTCAAGAGTTATGCGGGGTACATGGTGGAGTGCACAAGAGAGATGCTTCAATCAATAGAAAATGAGGTAAAATCGGGGCGATCCGAGTTTGAAATCGGCGAGTATATGACCAAACTCACTGCTGACATAATTTCCAGAACCGAGTTTGAAAGCAGTTATGAAAAGGGAAAACAAATCTTTCATTTGCTTACTGTTCTACAGCATCTCTGTGCCCAGGCCAGCCGCCATCTCTGCCTTCCCGGTAGCCGATTTTTTCCAAGTAAATATAACAGAGAGATAAAGGCATTGAAGGGGAAAGTGGAGGGATTGTTAATGGAGATAATACAAAGCAGAAGGGACTGTGTGGAGATTGGGAGGAGCAGTTCATATGGAAATGATTTATTGGGAATGTTGCTGAATGAGATGCAGAAGAAAAAAGATGGGAATGGTTTGAGTTTGAATTTGCAAATTATTATGGATGAATGCAAGACCTTCTTCTTTGCTGGCCATGAAACAACTGCTCTTTTGCTCACTTGGACTGTAATGTTATTAGCCACTAACCCTTCTTGGCAACAAAAGGTTCGAGCTGAAGTTATGACCGTTTGCAATGGCCAAACTCCCTCGCTCGATCATCTCTCCAAACTCTCCCTGTTGAGTATGGTGATAAATGAATCATTGCGACTATATCCACCGGCAAGTATTCTTCCAAGAATGGCATTTGAGGATATAAAATTGGGAGATCTTGAGATTCCGAAAGGATTGTCGATATGGATTCCAGTATTGGCCATTCACCACAGTGAAGAGTTATGGGGAAAAGATGCGAATGAGTTCAACCCAGAACGATTTAGCAATTCGAAAGCCTTTACGTCGGGGAGATTCATCCCTTTTGCTTCGGGCCCTCGAAACTGCGTCGGCCAATCGTTTGCTCTCATGGAAACCAAGATCATCTTGGCTATGCTCATCTCCAAGTTTAGCTTTACAATTTCGGACAATTATCGCCATGCACCTGTGGTTGTGCTTACTATAAAACCTAAATATGGCGTTCAAGTTTGCTTGAAGCCTTTGAATGAATAG

Protein sequence

MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLNE
Homology
BLAST of Spg039267 vs. NCBI nr
Match: XP_038890905.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 984.2 bits (2543), Expect = 4.4e-283
Identity = 488/520 (93.85%), Postives = 499/520 (95.96%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M +   L+LTTL L  TSFFIFSLLYDTLS YC TPRRIK+KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWS QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREML+SIENEVKSGRSEFEIGEYMT+LTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVE LLMEIIQSRRDCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ K+RA
Sbjct: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRA 360

Query: 361 EVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           EV+ +C  +TPS  HLSKLSLL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI
Sbjct: 361 EVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPVLAIHHSEE+WGKDANEFNPERF+NSKAFTSG FIPFASGPRNCVGQSFALMETKII
Sbjct: 421 WIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           LAMLISKFSFTISD YRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 LAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 520

BLAST of Spg039267 vs. NCBI nr
Match: KAA0039372.1 (cytokinin hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 964.9 bits (2493), Expect = 2.8e-277
Identity = 483/522 (92.53%), Postives = 497/522 (95.21%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M +   L+LTTLAL  TSFFIFSLLYDT+S Y LTPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EML+SIE EVKSGRSEFEIGEYMT+LTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KV
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEVM VC  +TPS  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           SIWIPVLAIHHSEELWGKDANEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETK
Sbjct: 421 SIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Spg039267 vs. NCBI nr
Match: XP_008459472.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 963.8 bits (2490), Expect = 6.2e-277
Identity = 482/522 (92.34%), Postives = 497/522 (95.21%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M +   L+LTTLAL  TSFFIFSLLYDT+S Y LTPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EML+SIE EVKSGRSEFEIGEYMT+LTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KV
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEVM VC  +TPS  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           SIWIP+LAIHHSEELWGKDANEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETK
Sbjct: 421 SIWIPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Spg039267 vs. NCBI nr
Match: XP_011656036.1 (cytokinin hydroxylase [Cucumis sativus] >KAE8648965.1 hypothetical protein Csa_008948 [Cucumis sativus])

HSP 1 Score: 962.2 bits (2486), Expect = 1.8e-276
Identity = 483/522 (92.53%), Postives = 497/522 (95.21%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M +    +LTTLAL  TSFFIFSLLYDTLS Y  TPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLLPLLTTLALLTTSFFIFSLLYDTLSCYLFTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EML+S+E EVKSGRSEFEIGEYMT+LTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESLEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVE LLMEIIQSRRDCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KV
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQNKV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEVM VC  +TPS  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           SIWIPVLAIHHSEELWGKDANEFNPERF+NSK FTSG FIPFASGPRNCVGQSFALMETK
Sbjct: 421 SIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCVGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISD+YRHAPVVVLTIKPKYGVQVCL+PLN
Sbjct: 481 IILAMLISKFSFTISDSYRHAPVVVLTIKPKYGVQVCLEPLN 522

BLAST of Spg039267 vs. NCBI nr
Match: XP_022939611.1 (cytokinin hydroxylase-like isoform X1 [Cucurbita moschata] >KAG7016062.1 Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 954.1 bits (2465), Expect = 4.9e-274
Identity = 474/519 (91.33%), Postives = 493/519 (94.99%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           MA+  AL+LTTLA AATSF IFSLLY TLS Y LTPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MAIMLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREMLQS+ENEVKSGRSEFEIGEYMT+LTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Spg039267 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.1e-212
Identity = 365/502 (72.71%), Postives = 426/502 (84.86%), Query Frame = 0

Query: 21  IFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISH 80
           I  +LYDT+S Y LTPRRIK  M +QGV GPKPRPL GNIL+++A++S+S S D  SI H
Sbjct: 17  ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASKDCDSIHH 76

Query: 81  DLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGS 140
           D+V RLLPH++AWS Q+GKRFI WNG +PR+CLTET+LIKELL K++ VSG+SWLQQQG+
Sbjct: 77  DIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQQQGT 136

Query: 141 KHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIENEVKSGRSE 200
           K+FIGRGLLMANGQ+W HQRH+ APAF G+RLK YA +MVECT ++++ +  EV  G +E
Sbjct: 137 KNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERLRKEVGEGANE 196

Query: 201 FEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKY 260
            EIGE M KLTADIISRT+F SS+EKGK++F+ LTVLQ  CAQA+RHLC PGSRF PSKY
Sbjct: 197 VEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFLPSKY 256

Query: 261 NREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEMQKKKDGNGLSLNLQ 320
           NREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+LLNEM   K+ N  + NLQ
Sbjct: 257 NREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQ 316

Query: 321 IIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVC--NGQTPSLDHLSK 380
           +IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR EV  V   NG  PS+D LSK
Sbjct: 317 LIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNG-LPSVDQLSK 376

Query: 381 LSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDA 440
           L+ LS VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWIPVLAIHHSEELWGKDA
Sbjct: 377 LTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDA 436

Query: 441 NEFNPERFSNSKAFTSGR-FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYR 500
           N+FNPERF   + F SGR FIPFA+GPRNC+GQ FALME KIILA LISKF+FTIS NYR
Sbjct: 437 NQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYR 496

Query: 501 HAPVVVLTIKPKYGVQVCLKPL 520
           HAP+VVLTIKPKYGVQV LKPL
Sbjct: 497 HAPIVVLTIKPKYGVQVILKPL 516

BLAST of Spg039267 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 1.4e-202
Identity = 353/521 (67.75%), Postives = 419/521 (80.42%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M VT  L      L      I  +LYD++  Y LTPRRIK  M +QG+ GPKPR L GNI
Sbjct: 1   MMVTLVL---KYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           +D++ +LS S S+D  SI H++VPRLLPH+++WS Q+GKRFI WNG EPR+CLTET++IK
Sbjct: 61  IDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELL+K++ V+GKSWLQQQG+K FIGRGLLMANG+ W HQRH+ APAF  DRLK YA +MV
Sbjct: 121 ELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+ M + +  EV     E EIGE M +LTADIISRTEF SS +KGK++F LLTVLQ L
Sbjct: 181 ECTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQA+RHLC PGSRF PSKYNREIK+LK +VE LLMEII SR+D VEIGRSSSYG+DLLG
Sbjct: 241 CAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           +LLN+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  VR 
Sbjct: 301 LLLNQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRD 360

Query: 361 EVMTVCNGQ-TPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS 420
           EV  VC     PS++ LS L+ L+ VINESLRLYPPA++LPRMAFEDIKLGDL IPKGLS
Sbjct: 361 EVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKI 480
           IWIPVLAIHHS ELWG+DANEFNPERF+     +S  F+PFA+GPRNC+GQ+FA+ME KI
Sbjct: 421 IWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKI 480

Query: 481 ILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ILAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 481 ILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Spg039267 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.3e-99
Identity = 201/531 (37.85%), Postives = 315/531 (59.32%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           + +  AL++  L +    F  F +L      + LT RR+KN    QG+ GP  R   GN+
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMIL--VWHPFVLT-RRLKN----QGISGPNYRIFYGNL 62

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
            ++  +  +S  S +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C+++ +L K
Sbjct: 63  SEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAK 122

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
            +LS       KS  + +  K    +GL+   G +W+  R I+ PAF  DRLK     MV
Sbjct: 123 TMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMV 182

Query: 181 ECTREML-----QSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLT 240
           +CT +ML     +S + E +  + + E+ E   +LTADII+ + F SSY +G ++F    
Sbjct: 183 DCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQM 242

Query: 241 VLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYG 300
            L+     +   + +PG+++ P+  N  +  L+ K++  +  II SR     +   S YG
Sbjct: 243 ELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYG 302

Query: 301 NDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ 360
           +DLLG+LL       +G    ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ
Sbjct: 303 DDLLGILLKAY--NTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQ 362

Query: 361 QKVRAEVMTVCNGQ-TPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEI 420
           +K+R E+   C  + TP  +  SKL L++MVI ESLRLY P S L R A  +IKLGDLEI
Sbjct: 363 EKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEI 422

Query: 421 PKGLSIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGR---FIPFASGPRNCVGQS 480
           PKG ++ IP+L +H  + LWG DA++FNP RF+N  +  +      + F+ GPR C+GQ+
Sbjct: 423 PKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQN 482

Query: 481 FALMETKIILAMLISKFSF-TISDNYRHAPVVVLTIKPKYGVQVCLKPLNE 522
           F ++E K +L M++ +F F ++ D Y+H PV  +TI+P+YG+ V L+PL +
Sbjct: 483 FVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLED 519

BLAST of Spg039267 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 6.3e-99
Identity = 200/526 (38.02%), Postives = 306/526 (58.17%), Query Frame = 0

Query: 8   VLTTLALAATSFFIFSL--LYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           +L+T+ L A +  +  +  +Y         P  +  +  KQG+ GPK R L GN+ ++  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           + +++    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS 
Sbjct: 118 MKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSN 177

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
                 KS  + +  K   G GL+  NG +W+  R I+ PAF  D+LK     MV+CT  
Sbjct: 178 KFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 188 MLQSIENEVKSGRSE--FEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 247
           M    + +     +E    I     +LTADII+   F SSY +G ++F     LQ  CA 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 248 ASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 307
           A   L  PG ++ P+  N +I  L  KV   +  II +R        S  YGNDLLG++L
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIML 357

Query: 308 -----NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 367
                NE +KK       +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ+K+
Sbjct: 358 TAASSNESEKK-------MSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKL 417

Query: 368 RAEVMTVC-NGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 427
           R EV   C   + P  +  SKL L++ V  ESLRLY P   L R+A ED+KLG+LEIPKG
Sbjct: 418 REEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKG 477

Query: 428 LSIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGR---FIPFASGPRNCVGQSFAL 487
            +I +P+  +H  + +WG DA++FNP RF+N  +  +      + F+ GPR C+GQ+FA+
Sbjct: 478 TTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAI 537

Query: 488 METKIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ME K +LAM++ +F   +S +Y+HAP   LT++P+Y + V L+P++
Sbjct: 538 MEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Spg039267 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.1e-94
Identity = 197/513 (38.40%), Postives = 294/513 (57.31%), Query Frame = 0

Query: 21  IFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAAL--LSKST------S 80
           +FS +Y  L    L P R++ K+  QGVRGPKP  L GNI ++  +  L+KS       S
Sbjct: 22  LFSYVYIIL---WLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEAGS 81

Query: 81  SDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGK 140
           +DM   S + V  L P+FL WS  +G  +++  G    + +T+ +++KEL +  S   GK
Sbjct: 82  TDM--FSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANCKSLDLGK 141

Query: 141 SWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIEN 200
               Q+     +G G+L +NG  W+HQR ++AP    +R+K     M+E    ML S +N
Sbjct: 142 PCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKN 201

Query: 201 EV--KSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCL 260
           EV  + G +E  + E++   +AD+ISR  F SS+ +GK+IF  +  LQ   A+ S  + +
Sbjct: 202 EVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGV 261

Query: 261 PGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEMQKKK 320
           PGSR+ P++ NR I  L   +  L++ I +      E   S+S   DLL  ++   Q  K
Sbjct: 262 PGSRYLPTRSNRGIWNLDSSIRTLILNISKK----YEHDSSTSVNKDLLHSII---QGSK 321

Query: 321 DGNGLSLNLQ-IIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVCNGQ 380
           DG   S   +  I+D CK  +FAGHETT+    W +MLLA++  WQ + R E + +C G+
Sbjct: 322 DGPFASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGR 381

Query: 381 TPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHH 440
               D L KL  L+MVI E+LRLYPPAS + R A  D+KLG ++IPKG +IWIP+   H 
Sbjct: 382 PLDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHR 441

Query: 441 SEELWGKDANEFNPERFSNSKAFT---SGRFIPFASGPRNCVGQSFALMETKIILAMLIS 500
              +WG  A++F+P+RF+N  A        ++PF  G R C GQ+ A++E K++L++L+S
Sbjct: 442 DPSVWGPSADKFDPDRFANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLS 501

Query: 501 KFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 520
           KF F +S NY H P   LTI+P  GV +  + L
Sbjct: 502 KFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Spg039267 vs. ExPASy TrEMBL
Match: A0A5A7T7B8 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001740 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.3e-277
Identity = 483/522 (92.53%), Postives = 497/522 (95.21%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M +   L+LTTLAL  TSFFIFSLLYDT+S Y LTPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EML+SIE EVKSGRSEFEIGEYMT+LTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KV
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEVM VC  +TPS  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           SIWIPVLAIHHSEELWGKDANEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETK
Sbjct: 421 SIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Spg039267 vs. ExPASy TrEMBL
Match: A0A1S3CAS2 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.0e-277
Identity = 482/522 (92.34%), Postives = 497/522 (95.21%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M +   L+LTTLAL  TSFFIFSLLYDT+S Y LTPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EML+SIE EVKSGRSEFEIGEYMT+LTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KV
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEVM VC  +TPS  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           SIWIP+LAIHHSEELWGKDANEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETK
Sbjct: 421 SIWIPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Spg039267 vs. ExPASy TrEMBL
Match: A0A6J1FGF0 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.4e-274
Identity = 474/519 (91.33%), Postives = 493/519 (94.99%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           MA+  AL+LTTLA AATSF IFSLLY TLS Y LTPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MAIMLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREMLQS+ENEVKSGRSEFEIGEYMT+LTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Spg039267 vs. ExPASy TrEMBL
Match: A0A6J1JYD2 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.5e-273
Identity = 473/519 (91.14%), Postives = 491/519 (94.61%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           MA   AL+LTTLA A TSF IFSLLY TLS Y LTPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MATMLALLLTTLAFAVTSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREMLQS+ENEVKSGRSEFEIGEYMT+LTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Spg039267 vs. ExPASy TrEMBL
Match: A0A6J1FHA5 (cytokinin hydroxylase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.1e-266
Identity = 466/519 (89.79%), Postives = 485/519 (93.45%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           MA+  AL+LTTLA AATSF IFSLLY TLS Y LTPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MAIMLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW HQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREMLQS+ENEVKSGRSEFEIGEYMT+LTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGS        REIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGS--------REIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVMTVCNGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 511

BLAST of Spg039267 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 740.7 bits (1911), Expect = 7.9e-214
Identity = 365/502 (72.71%), Postives = 426/502 (84.86%), Query Frame = 0

Query: 21  IFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISH 80
           I  +LYDT+S Y LTPRRIK  M +QGV GPKPRPL GNIL+++A++S+S S D  SI H
Sbjct: 17  ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASKDCDSIHH 76

Query: 81  DLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGS 140
           D+V RLLPH++AWS Q+GKRFI WNG +PR+CLTET+LIKELL K++ VSG+SWLQQQG+
Sbjct: 77  DIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQQQGT 136

Query: 141 KHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIENEVKSGRSE 200
           K+FIGRGLLMANGQ+W HQRH+ APAF G+RLK YA +MVECT ++++ +  EV  G +E
Sbjct: 137 KNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERLRKEVGEGANE 196

Query: 201 FEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKY 260
            EIGE M KLTADIISRT+F SS+EKGK++F+ LTVLQ  CAQA+RHLC PGSRF PSKY
Sbjct: 197 VEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFLPSKY 256

Query: 261 NREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEMQKKKDGNGLSLNLQ 320
           NREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+LLNEM   K+ N  + NLQ
Sbjct: 257 NREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQ 316

Query: 321 IIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVC--NGQTPSLDHLSK 380
           +IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR EV  V   NG  PS+D LSK
Sbjct: 317 LIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNG-LPSVDQLSK 376

Query: 381 LSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDA 440
           L+ LS VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWIPVLAIHHSEELWGKDA
Sbjct: 377 LTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDA 436

Query: 441 NEFNPERFSNSKAFTSGR-FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYR 500
           N+FNPERF   + F SGR FIPFA+GPRNC+GQ FALME KIILA LISKF+FTIS NYR
Sbjct: 437 NQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYR 496

Query: 501 HAPVVVLTIKPKYGVQVCLKPL 520
           HAP+VVLTIKPKYGVQV LKPL
Sbjct: 497 HAPIVVLTIKPKYGVQVILKPL 516

BLAST of Spg039267 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 707.2 bits (1824), Expect = 9.7e-204
Identity = 353/521 (67.75%), Postives = 419/521 (80.42%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           M VT  L      L      I  +LYD++  Y LTPRRIK  M +QG+ GPKPR L GNI
Sbjct: 1   MMVTLVL---KYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
           +D++ +LS S S+D  SI H++VPRLLPH+++WS Q+GKRFI WNG EPR+CLTET++IK
Sbjct: 61  IDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
           ELL+K++ V+GKSWLQQQG+K FIGRGLLMANG+ W HQRH+ APAF  DRLK YA +MV
Sbjct: 121 ELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMV 180

Query: 181 ECTREMLQSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+ M + +  EV     E EIGE M +LTADIISRTEF SS +KGK++F LLTVLQ L
Sbjct: 181 ECTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQA+RHLC PGSRF PSKYNREIK+LK +VE LLMEII SR+D VEIGRSSSYG+DLLG
Sbjct: 241 CAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRA 360
           +LLN+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  VR 
Sbjct: 301 LLLNQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRD 360

Query: 361 EVMTVCNGQ-TPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS 420
           EV  VC     PS++ LS L+ L+ VINESLRLYPPA++LPRMAFEDIKLGDL IPKGLS
Sbjct: 361 EVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKI 480
           IWIPVLAIHHS ELWG+DANEFNPERF+     +S  F+PFA+GPRNC+GQ+FA+ME KI
Sbjct: 421 IWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKI 480

Query: 481 ILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ILAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 481 ILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Spg039267 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 365.2 bits (936), Expect = 9.0e-101
Identity = 201/531 (37.85%), Postives = 315/531 (59.32%), Query Frame = 0

Query: 1   MAVTFALVLTTLALAATSFFIFSLLYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNI 60
           + +  AL++  L +    F  F +L      + LT RR+KN    QG+ GP  R   GN+
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMIL--VWHPFVLT-RRLKN----QGISGPNYRIFYGNL 62

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIK 120
            ++  +  +S  S +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C+++ +L K
Sbjct: 63  SEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAK 122

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMV 180
            +LS       KS  + +  K    +GL+   G +W+  R I+ PAF  DRLK     MV
Sbjct: 123 TMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMV 182

Query: 181 ECTREML-----QSIENEVKSGRSEFEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLT 240
           +CT +ML     +S + E +  + + E+ E   +LTADII+ + F SSY +G ++F    
Sbjct: 183 DCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQM 242

Query: 241 VLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYG 300
            L+     +   + +PG+++ P+  N  +  L+ K++  +  II SR     +   S YG
Sbjct: 243 ELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYG 302

Query: 301 NDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ 360
           +DLLG+LL       +G    ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ
Sbjct: 303 DDLLGILLKAY--NTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQ 362

Query: 361 QKVRAEVMTVCNGQ-TPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEI 420
           +K+R E+   C  + TP  +  SKL L++MVI ESLRLY P S L R A  +IKLGDLEI
Sbjct: 363 EKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEI 422

Query: 421 PKGLSIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGR---FIPFASGPRNCVGQS 480
           PKG ++ IP+L +H  + LWG DA++FNP RF+N  +  +      + F+ GPR C+GQ+
Sbjct: 423 PKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQN 482

Query: 481 FALMETKIILAMLISKFSF-TISDNYRHAPVVVLTIKPKYGVQVCLKPLNE 522
           F ++E K +L M++ +F F ++ D Y+H PV  +TI+P+YG+ V L+PL +
Sbjct: 483 FVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLED 519

BLAST of Spg039267 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 362.8 bits (930), Expect = 4.5e-100
Identity = 200/526 (38.02%), Postives = 306/526 (58.17%), Query Frame = 0

Query: 8   VLTTLALAATSFFIFSL--LYDTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           +L+T+ L A +  +  +  +Y         P  +  +  KQG+ GPK R L GN+ ++  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           + +++    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS 
Sbjct: 118 MKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSN 177

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWLHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
                 KS  + +  K   G GL+  NG +W+  R I+ PAF  D+LK     MV+CT  
Sbjct: 178 KFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 188 MLQSIENEVKSGRSE--FEIGEYMTKLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 247
           M    + +     +E    I     +LTADII+   F SSY +G ++F     LQ  CA 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 248 ASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 307
           A   L  PG ++ P+  N +I  L  KV   +  II +R        S  YGNDLLG++L
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIML 357

Query: 308 -----NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 367
                NE +KK       +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ+K+
Sbjct: 358 TAASSNESEKK-------MSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKL 417

Query: 368 RAEVMTVC-NGQTPSLDHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 427
           R EV   C   + P  +  SKL L++ V  ESLRLY P   L R+A ED+KLG+LEIPKG
Sbjct: 418 REEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKG 477

Query: 428 LSIWIPVLAIHHSEELWGKDANEFNPERFSNSKAFTSGR---FIPFASGPRNCVGQSFAL 487
            +I +P+  +H  + +WG DA++FNP RF+N  +  +      + F+ GPR C+GQ+FA+
Sbjct: 478 TTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAI 537

Query: 488 METKIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ME K +LAM++ +F   +S +Y+HAP   LT++P+Y + V L+P++
Sbjct: 538 MEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Spg039267 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 3.3e-95
Identity = 179/493 (36.31%), Postives = 287/493 (58.22%), Query Frame = 0

Query: 36  PRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSA 95
           PR+I+   +KQG+RGP     +GN+ ++  ++ K++S  MP  SH+++PR+L  +  W  
Sbjct: 35  PRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRK 94

Query: 96  QFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQN 155
            +G  F+ W G   R+ + + DLI+E+ SK               K   G GLL   G+ 
Sbjct: 95  IYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE--AHPLVKQLEGDGLLSLKGEK 154

Query: 156 WLHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIENEV-KSGRSEFEIGEYMTKLTADI 215
           W H R I++P F  + LK     +++   +M+    +++ ++G  E ++ E+   LT D+
Sbjct: 155 WAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTEDV 214

Query: 216 ISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGL 275
           ISRT F SSYE G+ +F L      LCA+A + + +PG RFFP++ N +   L  ++   
Sbjct: 215 ISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKS 274

Query: 276 LMEIIQSRRDCVEIGRSSS----YGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFF 335
           L+++I+ RR     G           DLLG+++            ++ +Q I++ECK+FF
Sbjct: 275 LLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---------QAKNVTVQDIVEECKSFF 334

Query: 336 FAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVCNGQ-TPSLDHLSKLSLLSMVINES 395
           FAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC  +  P+ DH+ KL  LSM++NES
Sbjct: 335 FAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNES 394

Query: 396 LRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPERFSNS 455
           LRLYPP     R A  D+KLG  +IP G  + IP++A+HH + +WG D NEFNP RF++ 
Sbjct: 395 LRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADG 454

Query: 456 KAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYRHAPVVVLTI 515
               +     FIPF  G R C+GQ+ A+++ K+ LA++I +F+F ++  Y+HAP V++ +
Sbjct: 455 VPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLL 514

Query: 516 KPKYGVQVCLKPL 520
            P++G  +  + L
Sbjct: 515 YPQHGAPITFRRL 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890905.14.4e-28393.85cytokinin hydroxylase-like [Benincasa hispida][more]
KAA0039372.12.8e-27792.53cytokinin hydroxylase-like [Cucumis melo var. makuwa][more]
XP_008459472.16.2e-27792.34PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
XP_011656036.11.8e-27692.53cytokinin hydroxylase [Cucumis sativus] >KAE8648965.1 hypothetical protein Csa_0... [more]
XP_022939611.14.9e-27491.33cytokinin hydroxylase-like isoform X1 [Cucurbita moschata] >KAG7016062.1 Cytokin... [more]
Match NameE-valueIdentityDescription
Q9FF181.1e-21272.71Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q9ZW951.4e-20267.75Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
Q9ASR31.3e-9937.85Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1[more]
F4IK456.3e-9938.02Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
Q2QYH72.1e-9438.40Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5A7T7B81.3e-27792.53Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3CAS23.0e-27792.34cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1[more]
A0A6J1FGF02.4e-27491.33cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A6J1JYD21.5e-27391.14cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11148899... [more]
A0A6J1FHA51.1e-26689.79cytokinin hydroxylase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
Match NameE-valueIdentityDescription
AT5G38450.17.9e-21472.71cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.19.7e-20467.75cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT2G46960.29.0e-10137.85cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46950.14.5e-10038.02cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT2G26710.13.3e-9536.31Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 384..395
score: 46.75
coord: 332..349
score: 40.96
coord: 458..467
score: 55.03
coord: 467..478
score: 42.46
IPR001128Cytochrome P450PFAMPF00067p450coord: 95..494
e-value: 3.4E-87
score: 293.1
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 210..228
score: 19.27
coord: 321..338
score: 29.73
coord: 467..490
score: 31.04
coord: 94..113
score: 22.62
coord: 341..367
score: 28.62
coord: 457..467
score: 49.55
coord: 424..448
score: 25.82
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 33..521
e-value: 2.9E-112
score: 377.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 50..518
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 7..515
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 7..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039267.1Spg039267.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen