Spg039050 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg039050
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450 89A2-like
Location	scaffold12: 1235391 .. 1236865 (-)
RNA-Seq Expression	Spg039050
Synteny	Spg039050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACCGCATGCAGTGAAAGAAGATACAATGTTTGCGAATTATTTCATTCCCAAAAATGGAACAGTGAATATCCTGGCCGTGGAGATGGGGTGGGATCCAGAAGTATGGGAGGATCCGATGGCGTTTAAGCCGGAGAGGTTCGTGAGCGGCGACGGAGAGGCGACATTGATGACGACGACGACGTTTGATGTAACATGTAGCAAGGAGATTCAGATGATGCCGTTTGGAGCAGGGAGGAGGATATGTCCAGGAGCTGGTTTGGCAATGCTTCATTTGGAATATTTTGTTGCAAATTTGGTTTGGCGATTTGAGTGGAAGGCTGTGGAAGGAGATGAAATTGATTTATCAGAGAAGATGGAATTCACCATTGTGATGAAGAAGCCTCTCAAAGCCCATATACATCCAAGGTTTTGAAGGATATGAATATCTATGTAACATATATTTGAAAATCAAAAGGAGAGGTTAAGGGGAAAAAAAAACTTAGCCTCTACTTGATAGATATATATCTATATTTTTTTTCAATACAACAAGAGCGAAGACTCGCCCAATGCTTGTTAGATAACGTACCAATTACCATCTCAAAAGTGTCATGGTTTGATGTTCACCGTACCTTTTCAATTGTTGAACTATGAAAAATGGGGAGGACTTCAAAAGAATGTATAATTACCTCAATAATTGTTAAGTAACGTTATGTTTTATAATGATTTGGTTTTAATTTTCTATTTTTGGAAATTGTTTTTGTTTTCTCACCATTTTAAAAAATTATTTTTATCTTTCTTAAACAAACATTTAAATTATTCTTAACCTAATTTAAGAAACAAAAATAAGTTTTTGAAAATCATCAACTTTTTTTTTTTTTGTTTTTAAAAGTTGACTTATATATTTTGAAAACATTTGCAAAAAGTAGATAACAAGATAAAGAAACTCAAAGGTGGAAGTACTAAAGTAGTGTTTGTTGATGCCAAAACTCGGACAGGAGACTCACCTGACCTTCACGTGCCACCAACGGTCAATTTGTATGAGAGGAGAGGACGTGACGTGCAAGACAAGCAAACACTGCACACCGGTGTGGTGCTTGCCACATCGACTCCTATGCCTAAGTCAGAAAACTGGTGAAAGAGCAAGAGGGGAAGTAGAGAGTTCAGAGAATGAATTTCGGGATCTCATCTTCAGTGACGAAGAGAGGTTTAAATACCTGCTCATGCTCCTAGGGTTTTTAGGAATTCAGAGACGTTTCAGGACAATCCAACAGAACCGGGGTGGCCAGAGGTAGCAGAGACCAAGCGGAGGCGACCGAGCTCAGCCCGCGCAAGCGGCTTGGCCCGACCCTTTGGTCTGGTCTTCCTCTGGGTCAGTTTCCTGGTCCTACCTCTGCCCGATTGTCCTCGTCAGCTTCTTGTGAATCGGAATGGTCCAAAATTGCTTATAACAGTGTTTATAAACTTAACTTTTGGAAAAGAAAACCCAAATAA

mRNA sequence

ATGTTACCGCATGCAGTGAAAGAAGATACAATGTTTGCGAATTATTTCATTCCCAAAAATGGAACAGTGAATATCCTGGCCGTGGAGATGGGGTGGGATCCAGAAGTATGGGAGGATCCGATGGCGTTTAAGCCGGAGAGGTTCGTGAGCGGCGACGGAGAGGCGACATTGATGACGACGACGACGTTTGATGTAACATGTAGCAAGGAGATTCAGATGATGCCGTTTGGAGCAGGGAGGAGGATATGTCCAGGAGCTGGTTTGGCAATGCTTCATTTGGAATATTTTGTTGCAAATTTGGTTTGGCGATTTGAGTGGAAGGCTGTGGAAGGAGATGAAATTGATTTATCAGAGAAGATGGAATTCACCATTGTGATGAAGAAGCCTCTCAAAGCCCATATACATCCAAGTCAGAAAACTGGTGAAAGAGCAAGAGGGGAAGTAGAGAGTTCAGAGAATGAATTTCGGGATCTCATCTTCAGTGACGAAGAGAGAGGTAGCAGAGACCAAGCGGAGGCGACCGAGCTCAGCCCGCGCAAGCGGCTTGGCCCGACCCTTTGGTCTGGTCTTCCTCTGGGTCAGTTTCCTGGTCCTACCTCTGCCCGATTGTCCTCGTCAGCTTCTTGTGAATCGGAATGGTCCAAAATTGCTTATAACAGTGTTTATAAACTTAACTTTTGGAAAAGAAAACCCAAATAA

Coding sequence (CDS)

ATGTTACCGCATGCAGTGAAAGAAGATACAATGTTTGCGAATTATTTCATTCCCAAAAATGGAACAGTGAATATCCTGGCCGTGGAGATGGGGTGGGATCCAGAAGTATGGGAGGATCCGATGGCGTTTAAGCCGGAGAGGTTCGTGAGCGGCGACGGAGAGGCGACATTGATGACGACGACGACGTTTGATGTAACATGTAGCAAGGAGATTCAGATGATGCCGTTTGGAGCAGGGAGGAGGATATGTCCAGGAGCTGGTTTGGCAATGCTTCATTTGGAATATTTTGTTGCAAATTTGGTTTGGCGATTTGAGTGGAAGGCTGTGGAAGGAGATGAAATTGATTTATCAGAGAAGATGGAATTCACCATTGTGATGAAGAAGCCTCTCAAAGCCCATATACATCCAAGTCAGAAAACTGGTGAAAGAGCAAGAGGGGAAGTAGAGAGTTCAGAGAATGAATTTCGGGATCTCATCTTCAGTGACGAAGAGAGAGGTAGCAGAGACCAAGCGGAGGCGACCGAGCTCAGCCCGCGCAAGCGGCTTGGCCCGACCCTTTGGTCTGGTCTTCCTCTGGGTCAGTTTCCTGGTCCTACCTCTGCCCGATTGTCCTCGTCAGCTTCTTGTGAATCGGAATGGTCCAAAATTGCTTATAACAGTGTTTATAAACTTAACTTTTGGAAAAGAAAACCCAAATAA

Protein sequence

MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTTTTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKMEFTIVMKKPLKAHIHPSQKTGERARGEVESSENEFRDLIFSDEERGSRDQAEATELSPRKRLGPTLWSGLPLGQFPGPTSARLSSSASCESEWSKIAYNSVYKLNFWKRKPK

Homology

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BLAST of Spg039050 vs. NCBI nr
Match: XP_038877784.1 (cytochrome P450 89A2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 239.2 bits (609), Expect = 3.6e-59
Identity = 114/136 (83.82%), Postives = 122/136 (89.71%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           MLPHAVKEDT   NYFIPKNG VNI AVEMGWDP+VWEDPMAFKPERF+S  G  T  T+
Sbjct: 385 MLPHAVKEDTTLENYFIPKNGIVNIYAVEMGWDPQVWEDPMAFKPERFMSNVGGET--TS 444

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           TTFD+T SKEI+MMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKA+EG E+DLSEKM
Sbjct: 445 TTFDITGSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAMEG-EVDLSEKM 504

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTIVMK PLKA+IHP
Sbjct: 505 EFTIVMKNPLKAYIHP 517

[Close]

BLAST of Spg039050 vs. NCBI nr
Match: XP_038877785.1 (cytochrome P450 89A2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 239.2 bits (609), Expect = 3.6e-59
Identity = 114/136 (83.82%), Postives = 122/136 (89.71%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           MLPHAVKEDT   NYFIPKNG VNI AVEMGWDP+VWEDPMAFKPERF+S  G  T  T+
Sbjct: 375 MLPHAVKEDTTLENYFIPKNGIVNIYAVEMGWDPQVWEDPMAFKPERFMSNVGGET--TS 434

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           TTFD+T SKEI+MMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKA+EG E+DLSEKM
Sbjct: 435 TTFDITGSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAMEG-EVDLSEKM 494

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTIVMK PLKA+IHP
Sbjct: 495 EFTIVMKNPLKAYIHP 507

[Close]

BLAST of Spg039050 vs. NCBI nr
Match: XP_038877782.1 (cytochrome P450 89A2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 239.2 bits (609), Expect = 3.6e-59
Identity = 114/136 (83.82%), Postives = 122/136 (89.71%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           MLPHAVKEDT   NYFIPKNG VNI AVEMGWDP+VWEDPMAFKPERF+S  G  T  T+
Sbjct: 388 MLPHAVKEDTTLENYFIPKNGIVNIYAVEMGWDPQVWEDPMAFKPERFMSNVGGET--TS 447

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           TTFD+T SKEI+MMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKA+EG E+DLSEKM
Sbjct: 448 TTFDITGSKEIKMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAMEG-EVDLSEKM 507

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTIVMK PLKA+IHP
Sbjct: 508 EFTIVMKNPLKAYIHP 520

[Close]

BLAST of Spg039050 vs. NCBI nr
Match: XP_022957787.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 239.2 bits (609), Expect = 3.6e-59
Identity = 112/136 (82.35%), Postives = 117/136 (86.03%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDTM  NYFIPKNGTVNILA EMGWDPEVWEDPMAFKPERFV G GEA  M  
Sbjct: 376 VLPHAVKEDTMLENYFIPKNGTVNILAAEMGWDPEVWEDPMAFKPERFVGGGGEAEAM-- 435

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
             FD+T SKEI+MMPFG GRR+CP AGLAMLHLEYFVANLVWRFEWKAVEG E+DLSEKM
Sbjct: 436 -AFDITGSKEIKMMPFGVGRRMCPAAGLAMLHLEYFVANLVWRFEWKAVEGVEVDLSEKM 495

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTIVM  PLK HI P
Sbjct: 496 EFTIVMNNPLKPHIRP 508

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BLAST of Spg039050 vs. NCBI nr
Match: KAG6605760.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 238.8 bits (608), Expect = 4.7e-59
Identity = 111/136 (81.62%), Postives = 118/136 (86.76%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDTM  NYFIPKNGTVNILA EMGWDPEVWEDPMAFKPERF+ G GEA     
Sbjct: 376 VLPHAVKEDTMLENYFIPKNGTVNILAAEMGWDPEVWEDPMAFKPERFMGGGGEA---EA 435

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           TTFD+T SKEI+MMPFG GRR+CP AGLAMLHL+YFVANLVWRFEWKAVEG E+DLSEKM
Sbjct: 436 TTFDITGSKEIKMMPFGVGRRMCPAAGLAMLHLQYFVANLVWRFEWKAVEGVEVDLSEKM 495

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTIVM  PLK HI P
Sbjct: 496 EFTIVMNNPLKPHIRP 508

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BLAST of Spg039050 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-43
Identity = 82/132 (62.12%), Postives = 103/132 (78.03%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPH+V EDT+   Y +PKNGT+N +  E+G DP  WE+PMAFKPERF+ G+ EA     
Sbjct: 375 LLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFKPERFM-GEEEAV---- 434

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
              D+T S+ I+MMPFGAGRRICPG GLAMLHLEY+VAN+V  F+WK V+G E+DL+EK+
Sbjct: 435 ---DLTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKL 494

Query: 121 EFTIVMKKPLKA 133
           EFT+VMK PLKA
Sbjct: 495 EFTVVMKHPLKA 498

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BLAST of Spg039050 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.9e-43
Identity = 80/137 (58.39%), Postives = 106/137 (77.37%), Query Frame = 0


Query: 4   HAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTTTTF 63
           H V  DT+   + IP+ GT+N +  EMG DP++WEDP+ FKPERF+  +GEA       F
Sbjct: 381 HKVTHDTVLGGFLIPRQGTINFMVGEMGRDPKIWEDPLTFKPERFLE-NGEA-----CDF 440

Query: 64  DVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKMEF- 123
           D+T ++EI+MMPFGAGRR+CPG  L++LHLEY+VANLVW+FEWK VEG+E+DLSEK +F 
Sbjct: 441 DMTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLVWKFEWKCVEGEEVDLSEKQQFI 500

Query: 124 TIVMKKPLKAHIHPSQK 140
           T+VMK P KA+I+P +K
Sbjct: 501 TMVMKNPFKANIYPRRK 511

[Close]

BLAST of Spg039050 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.0e-28
Identity = 61/134 (45.52%), Postives = 82/134 (61.19%), Query Frame = 0


Query: 2   LPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTTT 61
           L H+V E    A Y IP +  V      +  DP VW DP  F P+RF+SG  +A      
Sbjct: 370 LTHSVTEPVKLAGYDIPMDTNVEFFVHGISHDPNVWSDPEKFDPDRFLSGREDA------ 429

Query: 62  TFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEG-DEIDLSEKM 121
             D+T  KE++MMPFG GRRICPG G+A +H+   +A +V  FEW A  G +++D SEK+
Sbjct: 430 --DITGVKEVKMMPFGVGRRICPGLGMATVHVNLMLARMVQEFEWFAYPGNNKVDFSEKL 489

Query: 122 EFTIVMKKPLKAHI 135
           EFT+VMK PL+A +
Sbjct: 490 EFTVVMKNPLRAKV 495

[Close]

BLAST of Spg039050 vs. ExPASy Swiss-Prot
Match: Q9LZ31 (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 57/136 (41.91%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 2   LPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTTT 61
           L HAV E T  A Y IP    V +    +  DP +W +P  F P+RF+ G  +A      
Sbjct: 383 LTHAVMETTTLAGYDIPAGVNVEVYLPGISEDPRIWNNPKKFDPDRFMLGKEDA------ 442

Query: 62  TFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKA-VEGDEIDLSEKM 121
             D+T    ++M+PFG GRRICPG  +A +H+   +A +V  FEW A   G EID + K+
Sbjct: 443 --DITGISGVKMIPFGVGRRICPGLAMATIHVHLMLARMVQEFEWCAHPPGSEIDFAGKL 502

Query: 122 EFTIVMKKPLKAHIHP 137
           EFT+VMK PL+A + P
Sbjct: 503 EFTVVMKNPLRAMVKP 510

[Close]

BLAST of Spg039050 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.0e-24
Identity = 59/146 (40.41%), Postives = 84/146 (57.53%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +L HAV E T    Y IP +  V +    +  DP+ W +P  F PERF+SG  EA     
Sbjct: 377 VLTHAVTEPTTLGGYDIPIDANVEVYTPAIAEDPKNWLNPEKFDPERFISGGEEA----- 436

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGD-EIDLSEK 120
              D+T    ++MMPFG GRRICPG  +A +H+   +A +V  FEW A   + ++D + K
Sbjct: 437 ---DITGVTGVKMMPFGVGRRICPGLAMATVHIHLMMARMVQEFEWGAYPPEKKMDFTGK 496

Query: 121 MEFTIVMKKPLKAHIHPSQKTGERAR 146
            EFT+VMK+ L+A I P  + GE+ +
Sbjct: 497 WEFTVVMKESLRATIKP--RGGEKVK 512

[Close]

BLAST of Spg039050 vs. ExPASy TrEMBL
Match: A0A6J1H044 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111459225 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.7e-59
Identity = 112/136 (82.35%), Postives = 117/136 (86.03%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDTM  NYFIPKNGTVNILA EMGWDPEVWEDPMAFKPERFV G GEA  M  
Sbjct: 376 VLPHAVKEDTMLENYFIPKNGTVNILAAEMGWDPEVWEDPMAFKPERFVGGGGEAEAM-- 435

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
             FD+T SKEI+MMPFG GRR+CP AGLAMLHLEYFVANLVWRFEWKAVEG E+DLSEKM
Sbjct: 436 -AFDITGSKEIKMMPFGVGRRMCPAAGLAMLHLEYFVANLVWRFEWKAVEGVEVDLSEKM 495

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTIVM  PLK HI P
Sbjct: 496 EFTIVMNNPLKPHIRP 508

[Close]

BLAST of Spg039050 vs. ExPASy TrEMBL
Match: A0A5A7TME8 (Cytochrome P450 89A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00750 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.9e-59
Identity = 107/136 (78.68%), Postives = 122/136 (89.71%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDT+F NYFIPKNG V+I AV MGWDP+VWEDPMAFKPERF+S DG      +
Sbjct: 371 ILPHAVKEDTIFENYFIPKNGIVSIFAVGMGWDPQVWEDPMAFKPERFMSDDGGREAGYS 430

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           TTFD+T +KEI+MMPFGAGRR+CP A LAMLHLEYFVANL+WRFEWKAVEG+E+DLSEKM
Sbjct: 431 TTFDITGNKEIKMMPFGAGRRMCPAASLAMLHLEYFVANLIWRFEWKAVEGEEVDLSEKM 490

Query: 121 EFTIVMKKPLKAHIHP 137
           EFT+VMKKPLKAHI+P
Sbjct: 491 EFTVVMKKPLKAHINP 506

[Close]

BLAST of Spg039050 vs. ExPASy TrEMBL
Match: A0A1S3ATZ4 (cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103482935 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.9e-59
Identity = 107/136 (78.68%), Postives = 122/136 (89.71%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDT+F NYFIPKNG V+I AV MGWDP+VWEDPMAFKPERF+S DG      +
Sbjct: 389 ILPHAVKEDTIFENYFIPKNGIVSIFAVGMGWDPQVWEDPMAFKPERFMSDDGGREAGYS 448

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           TTFD+T +KEI+MMPFGAGRR+CP A LAMLHLEYFVANL+WRFEWKAVEG+E+DLSEKM
Sbjct: 449 TTFDITGNKEIKMMPFGAGRRMCPAASLAMLHLEYFVANLIWRFEWKAVEGEEVDLSEKM 508

Query: 121 EFTIVMKKPLKAHIHP 137
           EFT+VMKKPLKAHI+P
Sbjct: 509 EFTVVMKKPLKAHINP 524

[Close]

BLAST of Spg039050 vs. ExPASy TrEMBL
Match: A0A6J1E305 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430325 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 6.6e-59
Identity = 110/136 (80.88%), Postives = 119/136 (87.50%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDT   NYFIPKNGTVNI++ +MGWDPEVWEDPM FKPERFVSGDGEA    +
Sbjct: 383 ILPHAVKEDTTLENYFIPKNGTVNIMSGDMGWDPEVWEDPMTFKPERFVSGDGEA---LS 442

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           T FD+T SKEI+MMPFG GRRICPG GLAMLHLEYFVANLVWRFEWKAVEGDE+DLSEKM
Sbjct: 443 TMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKM 502

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTI MK PLKA + P
Sbjct: 503 EFTIWMKNPLKAQLRP 515

[Close]

BLAST of Spg039050 vs. ExPASy TrEMBL
Match: A0A6J1I6F9 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111471545 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 8.6e-59
Identity = 109/136 (80.15%), Postives = 120/136 (88.24%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPHAVKEDT   NYFIPKNGTVNI++ EMGWDPEVWEDPM FKPERF+SGDGE   +++
Sbjct: 383 ILPHAVKEDTTLENYFIPKNGTVNIMSGEMGWDPEVWEDPMTFKPERFMSGDGE---VSS 442

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
           T FD+T SKEI+MMPFG GRRICPG GLAMLHLEYFVANLVWRFEWKAVEGDE+DLSEKM
Sbjct: 443 TMFDITGSKEIKMMPFGVGRRICPGYGLAMLHLEYFVANLVWRFEWKAVEGDEVDLSEKM 502

Query: 121 EFTIVMKKPLKAHIHP 137
           EFTI MK PLKA + P
Sbjct: 503 EFTIWMKNPLKAQLRP 515

[Close]

BLAST of Spg039050 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 180.3 bits (456), Expect = 1.8e-45
Identity = 83/132 (62.88%), Postives = 104/132 (78.79%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPH+V EDT+   Y +PK GT+N L  E+G DP+VWE+PMAFKPERF+ G+ EA     
Sbjct: 380 VLPHSVTEDTVLGGYKVPKKGTINFLVAEIGRDPKVWEEPMAFKPERFM-GEEEAV---- 439

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
              D+T S+ I+MMPFGAGRRICPG GLAMLHLEY+VAN+V  F+WK VEG E+DL+EK+
Sbjct: 440 ---DITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVEGHEVDLTEKV 499

Query: 121 EFTIVMKKPLKA 133
           EFT++MK PLKA
Sbjct: 500 EFTVIMKHPLKA 503

[Close]

BLAST of Spg039050 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 177.9 bits (450), Expect = 9.1e-45
Identity = 80/132 (60.61%), Postives = 101/132 (76.52%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPH+V EDT+   Y +PKNGT+N +  E+G DP+VWE+PMAFKPERF+           
Sbjct: 381 VLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPKVWEEPMAFKPERFME---------- 440

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
              D+T S+ I+MMPFGAGRRICPG GLAMLHLEY+VAN+V  F+WK V+G E+DL+EK+
Sbjct: 441 EAVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFDWKEVQGHEVDLTEKL 500

Query: 121 EFTIVMKKPLKA 133
           EFT+VMK PLKA
Sbjct: 501 EFTVVMKHPLKA 502

[Close]

BLAST of Spg039050 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-44
Identity = 82/132 (62.12%), Postives = 103/132 (78.03%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPH+V EDT+   Y +PKNGT+N +  E+G DP  WE+PMAFKPERF+ G+ EA     
Sbjct: 375 LLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFKPERFM-GEEEAV---- 434

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
              D+T S+ I+MMPFGAGRRICPG GLAMLHLEY+VAN+V  F+WK V+G E+DL+EK+
Sbjct: 435 ---DLTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKL 494

Query: 121 EFTIVMKKPLKA 133
           EFT+VMK PLKA
Sbjct: 495 EFTVVMKHPLKA 498

[Close]

BLAST of Spg039050 vs. TAIR 10
Match: AT3G03470.1 (cytochrome P450, family 87, subfamily A, polypeptide 9 )

HSP 1 Score: 176.8 bits (447), Expect = 2.0e-44
Identity = 80/137 (58.39%), Postives = 106/137 (77.37%), Query Frame = 0


Query: 4   HAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTTTTF 63
           H V  DT+   + IP+ GT+N +  EMG DP++WEDP+ FKPERF+  +GEA       F
Sbjct: 381 HKVTHDTVLGGFLIPRQGTINFMVGEMGRDPKIWEDPLTFKPERFLE-NGEA-----CDF 440

Query: 64  DVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKMEF- 123
           D+T ++EI+MMPFGAGRR+CPG  L++LHLEY+VANLVW+FEWK VEG+E+DLSEK +F 
Sbjct: 441 DMTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLVWKFEWKCVEGEEVDLSEKQQFI 500

Query: 124 TIVMKKPLKAHIHPSQK 140
           T+VMK P KA+I+P +K
Sbjct: 501 TMVMKNPFKANIYPRRK 511

[Close]

BLAST of Spg039050 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 175.3 bits (443), Expect = 5.9e-44
Identity = 79/132 (59.85%), Postives = 100/132 (75.76%), Query Frame = 0


Query: 1   MLPHAVKEDTMFANYFIPKNGTVNILAVEMGWDPEVWEDPMAFKPERFVSGDGEATLMTT 60
           +LPH+V EDT+   Y +PK GT+N +  E+G DP+VWE+PMAFKPERF+           
Sbjct: 382 VLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRDPKVWEEPMAFKPERFME---------- 441

Query: 61  TTFDVTCSKEIQMMPFGAGRRICPGAGLAMLHLEYFVANLVWRFEWKAVEGDEIDLSEKM 120
              D+T S+ I+MMPFGAGRRICPG GLAMLHLEY+VAN+V  FEW+ V+G E+DL+EK+
Sbjct: 442 EAVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFEWQEVQGHEVDLTEKL 501

Query: 121 EFTIVMKKPLKA 133
           EFT+VMK PLKA
Sbjct: 502 EFTVVMKHPLKA 503

The following BLAST results are available for this feature:

BLAST of Spg039050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877784.1	3.6e-59	83.82	cytochrome P450 89A2-like isoform X2 [Benincasa hispida]	[more]
XP_038877785.1	3.6e-59	83.82	cytochrome P450 89A2-like isoform X3 [Benincasa hispida]	[more]
XP_038877782.1	3.6e-59	83.82	cytochrome P450 89A2-like isoform X1 [Benincasa hispida]	[more]
XP_022957787.1	3.6e-59	82.35	cytochrome P450 89A2-like [Cucurbita moschata]	[more]
KAG6605760.1	4.7e-59	81.62	Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg039050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42602	1.7e-43	62.12	Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2	[more]
Q9SRQ1	2.9e-43	58.39	Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1	[more]
P37123	2.0e-28	45.52	Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=...	[more]
Q9LZ31	2.7e-25	41.91	Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1	[more]
O48928	3.0e-24	40.41	Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1	[more]

BLAST of Spg039050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H044	1.7e-59	82.35	cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111459225 PE=3 SV=...	[more]
A0A5A7TME8	2.9e-59	78.68	Cytochrome P450 89A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3ATZ4	2.9e-59	78.68	cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103482935 PE=3 SV=1	[more]
A0A6J1E305	6.6e-59	80.88	cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111430325 PE=3 SV=...	[more]
A0A6J1I6F9	8.6e-59	80.15	cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111471545 PE=3 SV=1	[more]

BLAST of Spg039050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64930.1	1.8e-45	62.88	cytochrome P450, family 87, subfamily A, polypeptide 7	[more]
AT1G64950.1	9.1e-45	60.61	cytochrome P450, family 89, subfamily A, polypeptide 5	[more]
AT1G64900.1	1.2e-44	62.12	cytochrome P450, family 89, subfamily A, polypeptide 2	[more]
AT3G03470.1	2.0e-44	58.39	cytochrome P450, family 87, subfamily A, polypeptide 9	[more]
AT1G64940.1	5.9e-44	59.85	cytochrome P450, family 87, subfamily A, polypeptide 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 67..83 score: 37.56 coord: 31..49 score: 34.01 coord: 83..101 score: 27.73
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..147 e-value: 1.3E-36 score: 128.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..136
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..129 e-value: 2.0E-30 score: 106.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 159..184
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 159..176
None	No IPR available	PANTHER	PTHR24298:SF852	P450, PUTATIVE-RELATED	coord: 2..137
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 2..137
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 76..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg039050.1	Spg039050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen