Spg039022 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039022
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description3-phosphoinositide-dependent protein kinase-1
Locationscaffold12: 2606507 .. 2612523 (-)
RNA-Seq ExpressionSpg039022
SyntenySpg039022
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCTCTTCCCTCTTTCCTGGACGTCGAAGGAAACCAAATTCCAAGCTCGAAGGGTAGTTCTTTCTTGCTCTTCTTATCCTTCATCTCTCTAGTCCACCAATCGATTTCATTTGTCGCCGCGAGGTTATCTTCATCAATTCCATATTCAGTTTCTCAATCTTTGGAAGCGACGGAAACCGGCCATGGATGACGCACAAGCAATTTGGGAAGAGATCGACCGCTCTGAGAGGTATCGTGTCTAATTTTCTGAACAGACTTTCCTGAATTTTCTTCTTTTTTTTTACCGTAAAAGCGTGGTCGCATATCAGTCAATTGACTCCTTATGTTACGATTCGTTGATGGGCTTTGCAGCTATCTTGTGTGCTCCATGTATGAGGAAGCATTAGAGCTAGCTTCCTCCGTTTTGAAGCGTATTTATCGAGACAAAATTGGCTTTGAAAATGATATGTTGGAAGCAGCTGGTATGGTTTTGGTTCAATCTTTGAAGGAACTTGGAAGGTAGTGTTCTTATAAGTTACTATCGTTTATTTCATTTAGTTGTATTTTTAAACCTATGTTATTACCTATCACATTTGCTAGTTTATAATTCGTACAATAACCAAAGGAACACTGAGTTTTAGAGGGCTGCGTATTGAGTCAAATCCTAAAGGGCTAAAGTGATAATTATAGACTGTGATGTTTGTTGTGAAGCCTTTTGATCGTAATCTTGATTAATATATTGTTGATTGTATTAGTCAATTTTTTAAGATATAGTTTGCAGGACGTCGTTTCCTGATAGGTGGTGGCCAAATTTCAAACTTTCATATTACATTATTAGTATTTTTAATTGTTTAATGAAAAGATAGTTTATATTGTTAATTGTATATTAAACCAATTATTCAGTTCACTCGTACTTTCAGGACATCACATATAGTGGATGAGCTCAAAGTATCATTTGCCTCAGTTGCAGCCATCCCTGTTACTGTTCTTTTAACTGGGTAATCTCATTTACTTTCAAACACAATGTCAAGCTAGTCTTGTAATTCATTTGTTTCTGTTTATGTTGTTTTAACTGGGTAATCTCATCTACTTCCAAACATAATGTCAAGCTAGTCTTGCAATTCATTTGTTTTTCCTGTCCGTTGATTGATCATGTAAGTACGTATCCAATGATCGTAATGGCCTAATGGGTAAATAAAAGATATTGGTTGATTTTAATACATGCTGAAATAAATTAGTGTCTCGAGAGGGACAACGTATGTATTGAATTAGAGATCTTTCTCTCTAGTCATTAACCAGATTTGAAATAGCTAGTCAATGATGCTATAAACCAATTGGACGGGTGGAGTGAGTAGAGTTTGCACACCCTGTAGAGAGAAGGCATCATCTACTTCCCGCTTCATACCTCTAATTACATTTGTAGAATGTTAGGCCCAATCGAGAATGGTAAAATTTATAATTATTTTGTTGTAGTCTCCCTATTTACATTTGTAGAATGCACACCCTGTAGAGAGAGGCATCTCTTGTTCGAAATTTTCTTTTTTCTTTAGGCTCCATATTTACTTGTTGTCCAGTTCACTATATCTTCCTTGTGTAATGTTGTCTTGTGTAGCTGGGAATTATTGGAGGTAGGCCATGAGTATAATTCAAGATATGCGACCCAATACCCCAACTATTTAGTAAAAAAGATGATTGTGAAGAAATAATCTCAATATACCTAAGATATCATGGGTTGGCCCAATAGTGGGAGGGCAAGGTGTAAACTTAGGTACCTTTATAGCTATAGATCATAACTTCCTGGCATTCTATCTAGAAAATTAATACTACGACTTTCCTAATGTTAAATGTTCTAGGATTAAGTAGTTGTCCTTTGAGCTCGGAGCTTTAGGTATTGGGAACCTGAGATTACGTAATGAGACTCTTTTGGCTAAGTGGTTGTGGTGGTTCTTTATAGAAGGTGATACTTTGTGGTATAAAGTGATTATGAGTAGGTATGACCCTCACCCTTTTGAATGTATCTCAAGTGGGGATTCAAGGGGTCTAATCAAAAACCTCTGGAAAGCTATCTCTTCTTGTTTCCCTTCTTTTTCGCAGTTTGTCAGGTGCTATGTGGTGGTAGGCTCTGATGTCTGTTTCTGGGAAGACCTTTGGGTGGGTGAAAACCTCTATGTATCTTGTTCCCTCGTCTCTATCTCTTGTCAGAGAACAGATTGCATTCAGTGGCCTGTGTGTTGCCTTCTCTCGACCTTACCTCTTTGATTTCTCTTGAGTTGCCCCCTACTGATAAGGAGGCAATTGATGTGGCATACCTCCTTTATGTTTTTCGTGATCAGTTTATTCATTTTGGGAGAAGGGATGACAGGGTTTGGTCCCCAGACCCATCTAGGAGTTTCTCTTGTCATTCCTATTTTCAGTCTTTGGTCCTTTCCCCTGACTCTCATGCTGCTTCTGAGACTTCTGTTTTTGTGTTACTCTAAAAGGTTAAAATTCCTAAGAAAGTGAGGTTTTTTTTTCTTGGCAAGTCTTACTTGGAAGGGTTAATACTTTGGACCGCGTTCAAAGACACTAATCTTTAGTTTTGTGTCCGCAATGGTGCATTCTTTGTAGAAGGCATGAGGAGAATTTGTATCATTTGTCATGGGAGTATCAGTTTGCTAACTACCTTTGAGATCGTTGGATGAGCTCGTCTGGTGTCAACGGGGCACATAATAGAGATGGGCGTTTTCTTTTGGAGGAGGTGCTTTTGCATCCCCCTTTAGGGATAAAGGCAAGCAAGATGTTGTGGTAGTCATGTTTTTTTGTAGTGTCGTGGGGTGTTTGGCTTGAGAGGAATAATAAGAGTTTTAGAGGAAAAGAGAAATCGAGTGAGGAAGTTTGGGAGGTAGTTAGATTTAATACTTCCTTGTGGGCGTTTGTCACTAGACCTTTTTGCAATTATGATCTTGGTCTGGTTCTTTTGGATTGGAGTCCCTTCTTGTAGTTAGTTTGGAGCTCCTTTTTTTTCTTGGGCTTGTTTTTTGTATGCCCTTGTATTCTTTCATCTTTTCAATGAAAGTTCAATTTCTAAAAAAAAAAAGGATTAAGTAGTTGTCCTTTGAAGTTTTTGAGTGTGGGCAAGTCATGCTGCTGTGAGAAAATTTTAATCGTTAATTTTACCTTGCTCTATTTCTCAGGGCATGTCTTCAGATCTCGGAAGGTCTTACTGATATGCAATGTTTTCTAGAAGAGTTCCTTAGCAAGTGGAATTTATTGAACAAAGAAATTTATGTTCTTGTTGGCTCAAGAAATATTGATGACAGAGAACATTGTGATGGGCATGCTCAGTTGACTGTTGATGAATATCTCCAAGTTGTTGAAGTTTACCTCCGGACGCTCATAGAGATAGGTTTGAAGGATGTGGATCTTGCTGTCTCTTGGGTTGAGAAAGCTGCTTTACCTGAGGAAAATCGTCAGGTAAATGGATCTTTTCATGTTATACTGAATGTCATTTAGATAGTTATAATTATAGGATTGATTGGACATCACAAGGTTTATGTTGTCAATTGATGGAGTTTTGATTTAAGATGAATTGGATATGAAATATGTCCGATCCATGTTATTGGCTTATCCTATGTCTTATTTATTCTTCTTCTCAATTGTTCCAACACTTTTCCTTTTCCAAGGAATCCAGCGTGGTATTAGATTGTTATTTTTTCCTTTTATTTTTTGATCTCTTACGTATACAACTTCATAATGCCTCTACTATTTTCTCAATATTTCATCAGTTCACTGCATCATTTTTCATCGTTATCAATGCATCTCAAAGAAAATCTCCAAGGAACTTTGACATTTGGGATGACGTATTTGATCCTATTTCTATATCTTTGTCAGTATTGTTGCATCCATCCCTCACCATAAGGATTTGAAAAAAATTGGGAGAGAAGTTTATGATTCAGATAAACGAAGTTTGTAAATTTCTGAAAATAATAATGACTGCACAAATATTTCTGAAAATAAAACGGACTGCACAAACTTTTCTATGAATTTTCAAACCTTGACTTAAAGCTACATTTGCATTTGTTACTGAATGTTATTTTCAACTAGTATTTGTCTGAATAACTTTATTGTCCGATTATAATCTTTCTTCTTTAATTTTAAACCGGAGAACTTTCTTGTAATTCACCTATAGGTGTTGGAAGTTTTACTCCGTTTATTTTATTCATCAATGAAATTTGTTTCTTTTCTAAAAAAAAAGAAAAAGAAAGAATAACCTCATTGTCTTCCTTTAGTCTTGAACTGAAAGTTTTTCTCCGTCAAAGTAAAGTTGCATCTCCCTCACATCCTGCCACCTTCTTTTTCCATTTTCTTATTGAGATAGTGGAGAGGAAAATGAGGAAATATTATTCATATCACAGAAAGTGAGGAAACCCCTCGAATATATGAGTACAACTCTGAACTTTAGTTAATAATCTGAATAATAACCAACTCTGGTTAAGAATTAACCAACACTGGTAACAGTAACAACTAATACACAGGTATTAAAACTAATGTAACTAATACATCTCCCTTCTTATTCACCTCCTGCCACCTTTTTCCATTTTCTTAATTGTTTTGCTTTCCTGTTCTTGATACCTAGTGCTTTGCCCAATAAATGTCAATTTTTTCCTTCTACTTCTTGGCTAGGTACTTTTGAGGAGGCTAGACTACCTGCAGTCTCTTAAAGCTGCTGGCCTGTCGCAATCATCCACTTCATCTTTGCTTAAAGATGAGTATAAAGCACATTTGTCTTCTTCAGAAGAACCTCAAATATCAAGAGCTTCAAAAGCAGCCGGAAACCCTGGATACCATCAAGATGGAGGGAATGCTACCAGAGAAACGGTTCTCAGACTACAGAAAGTTACAAAACCATGTTTTTGGCCGTTCCATGCTATTACTTTGAAGTTTGGAAGTACGCGGCTTGTCATATCCACCAGAAGGATTATGCTTAGCTGTTTGTCGGTGCTAATTTATTATCTATTGCGAAGGAAACTAACCACTTTGAAGAGGTATGTTTGTTGTCAAATAAAGAGCATGGTACTGATTCATCTCTCTTTCTACTAGCTATATCCATGTTAATTCCTTGCTGCAATGCCAAAATTGAGAAGTACGTGCAAGTTAGAAGTTCAACTTAAGAGATGAAGGATATTTTAGCTTAAGAAGTTTCTTTTGTAGGTTAAAGTTTTGTTAATTGTCTACTTTTAGGGATACATTACTTTGCAACAATTCTTAACAAAAAGTAATTGCATATTTGCATCTTACCGGCTAGACGAATTTGAAATATACTTATATACTTTCCTCTCAAGAAATAGGGAAAATGCCGGTAAGATTAAGGGTGAGTTTGGGGTGATTTTTGAAAAAAAAAAAGGTTTTAAATCAAAACACTTCTTTTTCATAAGCATTTTTGGAAGGAATCATAATCAAGTGCTACTTCTAGAATTCTCAACATCATTTTTACTATCTTATACGTGTTAAATTAAAAATTTAGAAAACTTTGAAAAGTGATTTCGGGGATAAAAGCACTTTTCACCCTTCTCTCTACTAATTGGTTAGGTTTTTTATTGGATTAAAACTTTTTTCTTTAACTTCTATGACTGACTAACTAATGTTGATACTACTGGCTGTTGTAAATGTTGTAATTAGGTGAGATTGGCAACCTTGTTCATATATACTTTACTTTTGATAATTGCAGCAATGATTGTATTGACATTTACGTTAAATGTGTTATTATCATTATTTCTTTTAATATCAGGATTGCTACAAGACAAGCTTCGTCCATAAAGAAAGCACTGGTAGACTTGTGGCAGCTTGCATTTTCATATCAAGTTAACCCTTTAGCTATTGCTCAACCCCTTTCTGGTGTAGCTCGTGGAGCCTCATGATTCTTTTTAAACCATATTTGTATTCTATCATTTGATATAAAGCAGCATTTGCAAGCATTTCTCTTTTCTGGGAATTCTCTTTCCCTTTGCTTCCTTGTGCTGTAGAACCTGCTGAAGAGTAAAATAAAGTTGAAGCTTAC

mRNA sequence

ATGGATGACGCACAAGCAATTTGGGAAGAGATCGACCGCTCTGAGAGCTATCTTGTGTGCTCCATGTATGAGGAAGCATTAGAGCTAGCTTCCTCCGTTTTGAAGCGTATTTATCGAGACAAAATTGGCTTTGAAAATGATATGTTGGAAGCAGCTGGTATGGTTTTGGTTCAATCTTTGAAGGAACTTGGAAGGACATCACATATAGTGGATGAGCTCAAAGTATCATTTGCCTCAGTTGCAGCCATCCCTGTTACTGTTCTTTTAACTGGCTGGGAATTATTGGAGAACAGATTGCATTCAGTGGCCTGTGTGTTGCCTTCTCTCGACCTTACCTCTTTGATTTCTCTTGAGTTGCCCCCTACTGATAAGGAGGCAATTGATGTGGCATACCTCCTTTATGTTTTTCGTGATCAGTTTATTCATTTTGGGAGAAGGGATGACAGGGTTTGGTCCCCAGACCCATCTAGGAGTTTCTCTTGTCATTCCTATTTTCAGTCTTTGGTCCTTTCCCCTGACTCTCATGCTGCTTCTGAGACTTCTTGTCGTGGGGTGTTTGGCTTGAGAGGAATAATAAGAGTTTTAGAGGAAAAGAGAAATCGAGTGAGGAAGTTTGGGAGGGCATGTCTTCAGATCTCGGAAGGTCTTACTGATATGCAATGTTTTCTAGAAGAGTTCCTTAGCAAGTGGAATTTATTGAACAAAGAAATTTATGTTCTTGTTGGCTCAAGAAATATTGATGACAGAGAACATTGTGATGGGCATGCTCAGTTGACTGTTGATGAATATCTCCAAGTTGTTGAAGTTTACCTCCGGACGCTCATAGAGATAGGTTTGAAGGATGTGGATCTTGCTGTCTCTTGGGTTGAGAAAGCTGCTTTACCTGAGGAAAATCGTCAGGTACTTTTGAGGAGGCTAGACTACCTGCAGTCTCTTAAAGCTGCTGGCCTGTCGCAATCATCCACTTCATCTTTGCTTAAAGATGAGTATAAAGCACATTTGTCTTCTTCAGAAGAACCTCAAATATCAAGAGCTTCAAAAGCAGCCGGAAACCCTGGATACCATCAAGATGGAGGGAATGCTACCAGAGAAACGGTTCTCAGACTACAGAAAGTTACAAAACCATGTTTTTGGCCGTTCCATGCTATTACTTTGAAGTTTGGAAGTACGCGGCTTGTCATATCCACCAGAAGGATTATGCTTAGCTGTTTGTCGGTGCTAATTTATTATCTATTGCGAAGGAAACTAACCACTTTGAAGAGGATTGCTACAAGACAAGCTTCGTCCATAAAGAAAGCACTGGTAGACTTGTGGCAGCTTGCATTTTCATATCAAGTTAACCCTTTAGCTATTGCTCAACCCCTTTCTGGTGTAGCTCGTGGAGCCTCATGA

Coding sequence (CDS)

ATGGATGACGCACAAGCAATTTGGGAAGAGATCGACCGCTCTGAGAGCTATCTTGTGTGCTCCATGTATGAGGAAGCATTAGAGCTAGCTTCCTCCGTTTTGAAGCGTATTTATCGAGACAAAATTGGCTTTGAAAATGATATGTTGGAAGCAGCTGGTATGGTTTTGGTTCAATCTTTGAAGGAACTTGGAAGGACATCACATATAGTGGATGAGCTCAAAGTATCATTTGCCTCAGTTGCAGCCATCCCTGTTACTGTTCTTTTAACTGGCTGGGAATTATTGGAGAACAGATTGCATTCAGTGGCCTGTGTGTTGCCTTCTCTCGACCTTACCTCTTTGATTTCTCTTGAGTTGCCCCCTACTGATAAGGAGGCAATTGATGTGGCATACCTCCTTTATGTTTTTCGTGATCAGTTTATTCATTTTGGGAGAAGGGATGACAGGGTTTGGTCCCCAGACCCATCTAGGAGTTTCTCTTGTCATTCCTATTTTCAGTCTTTGGTCCTTTCCCCTGACTCTCATGCTGCTTCTGAGACTTCTTGTCGTGGGGTGTTTGGCTTGAGAGGAATAATAAGAGTTTTAGAGGAAAAGAGAAATCGAGTGAGGAAGTTTGGGAGGGCATGTCTTCAGATCTCGGAAGGTCTTACTGATATGCAATGTTTTCTAGAAGAGTTCCTTAGCAAGTGGAATTTATTGAACAAAGAAATTTATGTTCTTGTTGGCTCAAGAAATATTGATGACAGAGAACATTGTGATGGGCATGCTCAGTTGACTGTTGATGAATATCTCCAAGTTGTTGAAGTTTACCTCCGGACGCTCATAGAGATAGGTTTGAAGGATGTGGATCTTGCTGTCTCTTGGGTTGAGAAAGCTGCTTTACCTGAGGAAAATCGTCAGGTACTTTTGAGGAGGCTAGACTACCTGCAGTCTCTTAAAGCTGCTGGCCTGTCGCAATCATCCACTTCATCTTTGCTTAAAGATGAGTATAAAGCACATTTGTCTTCTTCAGAAGAACCTCAAATATCAAGAGCTTCAAAAGCAGCCGGAAACCCTGGATACCATCAAGATGGAGGGAATGCTACCAGAGAAACGGTTCTCAGACTACAGAAAGTTACAAAACCATGTTTTTGGCCGTTCCATGCTATTACTTTGAAGTTTGGAAGTACGCGGCTTGTCATATCCACCAGAAGGATTATGCTTAGCTGTTTGTCGGTGCTAATTTATTATCTATTGCGAAGGAAACTAACCACTTTGAAGAGGATTGCTACAAGACAAGCTTCGTCCATAAAGAAAGCACTGGTAGACTTGTGGCAGCTTGCATTTTCATATCAAGTTAACCCTTTAGCTATTGCTCAACCCCTTTCTGGTGTAGCTCGTGGAGCCTCATGA

Protein sequence

MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSLKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELPPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASETSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVLVGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGNATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLKRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS
Homology
BLAST of Spg039022 vs. NCBI nr
Match: XP_023526235.1 (protein APEM9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.6 bits (1324), Expect = 8.8e-142
Identity = 293/463 (63.28%), Postives = 319/463 (68.90%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSL 60
           MDD +AIWE+I+RSESYLVCSMYEEAL LASSVLKRI ++K G END+LEAAGMVLVQSL
Sbjct: 1   MDDGEAIWEDIERSESYLVCSMYEEALGLASSVLKRISQEKNGCENDLLEAAGMVLVQSL 60

Query: 61  KELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELP 120
           KELGRTSH+VDELKVSFASVAAIPV VLLTG                             
Sbjct: 61  KELGRTSHMVDELKVSFASVAAIPVNVLLTG----------------------------- 120

Query: 121 PTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASET 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVL 240
                                      ACLQISEGL+DM+CFLEEFLSKW+LLN+EIYVL
Sbjct: 181 ---------------------------ACLQISEGLSDMRCFLEEFLSKWSLLNEEIYVL 240

Query: 241 VGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQ 300
            GSRNIDDREH D HAQLTVDEYLQVVEVYLRTL+EIGLKDVDLAVSWVEKAALPEE RQ
Sbjct: 241 AGSRNIDDREHYDDHAQLTVDEYLQVVEVYLRTLMEIGLKDVDLAVSWVEKAALPEEKRQ 300

Query: 301 VLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGN 360
           VLLRRLDY Q LKAA LSQSS SSL KD++KAHLSSSE+PQ SRASK A +PGYHQDGG+
Sbjct: 301 VLLRRLDYQQCLKAASLSQSSPSSLHKDDHKAHLSSSEDPQESRASKTALDPGYHQDGGS 347

Query: 361 ATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLK 420
           A RETVL+L K+TKP FWPF  ITLKFGSTRL+ISTRR++LSCL VLIYYLLRRKLTTLK
Sbjct: 361 ANRETVLKLHKLTKPWFWPFRTITLKFGSTRLIISTRRVLLSCLFVLIYYLLRRKLTTLK 347

Query: 421 RIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           R+A +Q  S+KKA+VDLWQLAFSYQVNPLAIAQPLSGV RGAS
Sbjct: 421 RMAQKQGLSLKKAVVDLWQLAFSYQVNPLAIAQPLSGVPRGAS 347

BLAST of Spg039022 vs. NCBI nr
Match: XP_022983646.1 (protein APEM9 [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 3.3e-141
Identity = 292/463 (63.07%), Postives = 318/463 (68.68%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSL 60
           MDD +AIWE+I+RSESYLVCSMYEEAL LASSVLKRI ++K G END+LEAAGMVLVQSL
Sbjct: 1   MDDGEAIWEDIERSESYLVCSMYEEALGLASSVLKRISQEKNGCENDLLEAAGMVLVQSL 60

Query: 61  KELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELP 120
           KELGRTSH+VDELKVSFASVA IPV VLLTG                             
Sbjct: 61  KELGRTSHMVDELKVSFASVAGIPVNVLLTG----------------------------- 120

Query: 121 PTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASET 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVL 240
                                      ACLQISEGL+DM+CFLEEFLSKW+LLN+EIYVL
Sbjct: 181 ---------------------------ACLQISEGLSDMRCFLEEFLSKWSLLNEEIYVL 240

Query: 241 VGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQ 300
            GSRNIDDREH D HAQLTVDEYLQVVEVYLRTL+EIGLKDVDLAVSWVEKAALPEE RQ
Sbjct: 241 AGSRNIDDREHYDDHAQLTVDEYLQVVEVYLRTLMEIGLKDVDLAVSWVEKAALPEEKRQ 300

Query: 301 VLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGN 360
           VLLRRLDY QSLKAA LSQSS SSL KD++KAHLSSSE+PQ SR SK A +PGYHQDGG+
Sbjct: 301 VLLRRLDYQQSLKAASLSQSSPSSLHKDDHKAHLSSSEDPQESRTSKTALDPGYHQDGGS 347

Query: 361 ATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLK 420
           A RETVL+L K+TKP FWPF  ITLKFGSTRLVISTR+++LSCL VLIYYLLRRKLTTLK
Sbjct: 361 ANRETVLKLHKLTKPWFWPFRTITLKFGSTRLVISTRKVVLSCLFVLIYYLLRRKLTTLK 347

Query: 421 RIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           R+A +Q  S+KKA+VDLWQLAFSYQVNPLAIAQPLSGV RGAS
Sbjct: 421 RMAQKQGLSVKKAVVDLWQLAFSYQVNPLAIAQPLSGVPRGAS 347

BLAST of Spg039022 vs. NCBI nr
Match: XP_022935649.1 (protein APEM9-like isoform X1 [Cucurbita moschata] >XP_022935650.1 protein APEM9-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 505.8 bits (1301), Expect = 4.1e-139
Identity = 289/463 (62.42%), Postives = 315/463 (68.03%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSL 60
           MDD +AIWE+I+RSESYLVCSMYEEAL LASSVLKRI ++  G END+LEAAGMVLVQSL
Sbjct: 1   MDDGEAIWEDIERSESYLVCSMYEEALGLASSVLKRISQENNGCENDLLEAAGMVLVQSL 60

Query: 61  KELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELP 120
           KELGRTSH+VDELKVSF SV AIPV VLLTG                             
Sbjct: 61  KELGRTSHMVDELKVSFTSVTAIPVNVLLTG----------------------------- 120

Query: 121 PTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASET 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVL 240
                                      ACLQISEGL+DM+CFLEEFLSKW+LLN+EIYVL
Sbjct: 181 ---------------------------ACLQISEGLSDMRCFLEEFLSKWSLLNEEIYVL 240

Query: 241 VGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQ 300
            G+RNIDDREH D HAQLTVDEYLQVVEVYL+TL+EIGLKDVDLAVSWVEKAALPEE RQ
Sbjct: 241 AGARNIDDREHYDDHAQLTVDEYLQVVEVYLQTLMEIGLKDVDLAVSWVEKAALPEEKRQ 300

Query: 301 VLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGN 360
           VLLRRLDY QSLKAA LSQSS SSL KD++KAHLSSSE+ Q SRASK A +PGYH DGGN
Sbjct: 301 VLLRRLDYQQSLKAASLSQSSPSSLHKDDHKAHLSSSEDRQESRASKTALDPGYHPDGGN 347

Query: 361 ATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLK 420
           A RETVL+L K+TKP FWPF  ITLKFGSTRL+ISTRR++LSCL VLIYYLLRRKLTTLK
Sbjct: 361 ANRETVLKLHKLTKPWFWPFRTITLKFGSTRLIISTRRVLLSCLFVLIYYLLRRKLTTLK 347

Query: 421 RIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           R+A +QA S KKA+VDLWQLAFSYQVNPLAIAQPLSGV RGAS
Sbjct: 421 RMAQKQALSAKKAMVDLWQLAFSYQVNPLAIAQPLSGVPRGAS 347

BLAST of Spg039022 vs. NCBI nr
Match: KAG6581201.1 (Protein APEM9, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017940.1 Protein APEM9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 505.0 bits (1299), Expect = 7.0e-139
Identity = 289/463 (62.42%), Postives = 317/463 (68.47%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSL 60
           MDD +AIWE+I+RSESYLVCSMYEEAL LASSVLKRI ++K   END+LEAAGMVLVQSL
Sbjct: 1   MDDGEAIWEDIERSESYLVCSMYEEALGLASSVLKRISQEKNVCENDLLEAAGMVLVQSL 60

Query: 61  KELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELP 120
           KELGRTSH+VDELKVSFASVAAIPV VLLTG                             
Sbjct: 61  KELGRTSHMVDELKVSFASVAAIPVNVLLTG----------------------------- 120

Query: 121 PTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASET 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVL 240
                                      ACLQISEG++DM+CFLEEFLSKW+LLN+EIYVL
Sbjct: 181 ---------------------------ACLQISEGISDMRCFLEEFLSKWSLLNEEIYVL 240

Query: 241 VGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQ 300
            GSRNIDD E  D HAQLTVDEYLQVVEV+L+TL+EIGLKDVDLAVSWVEKAALPEE RQ
Sbjct: 241 AGSRNIDDSERYDDHAQLTVDEYLQVVEVHLQTLMEIGLKDVDLAVSWVEKAALPEEKRQ 300

Query: 301 VLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGN 360
           VLLRRLDY QSLKAA LSQSS SSL KD++KAHLSSSE+PQ SRASK A +PGYH DGGN
Sbjct: 301 VLLRRLDYHQSLKAASLSQSSPSSLHKDDHKAHLSSSEDPQESRASKTALDPGYHPDGGN 347

Query: 361 ATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLK 420
           A RETVL+L K+TKP FWPF  ITLKFGSTRL+ISTRR++LSCL VLIYYLLRRKLTTLK
Sbjct: 361 ANRETVLKLHKLTKPWFWPFRTITLKFGSTRLIISTRRVLLSCLFVLIYYLLRRKLTTLK 347

Query: 421 RIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           R+A +QA S+KKA+VDLWQLAFSYQVNPLAIAQPLSGV RGAS
Sbjct: 421 RMAQKQALSVKKAMVDLWQLAFSYQVNPLAIAQPLSGVPRGAS 347

BLAST of Spg039022 vs. NCBI nr
Match: XP_022143437.1 (protein APEM9 [Momordica charantia])

HSP 1 Score: 483.0 bits (1242), Expect = 2.8e-132
Identity = 286/464 (61.64%), Postives = 309/464 (66.59%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFEN-DMLEAAGMVLVQS 60
           MDDA+AIWEEI+RSESYLVCSMYEEAL LA S+L RI +DK  +EN DMLEAAGMVLVQS
Sbjct: 1   MDDAKAIWEEIERSESYLVCSMYEEALSLAYSILTRISQDKSSWENGDMLEAAGMVLVQS 60

Query: 61  LKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLEL 120
           LKELGRTS IVDELK+SFASVAAIPV VLLTG                            
Sbjct: 61  LKELGRTSRIVDELKLSFASVAAIPVNVLLTG---------------------------- 120

Query: 121 PPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASE 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 TSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYV 240
                                       ACLQISEG +DM+ FLEEFLSKW LLN EIYV
Sbjct: 181 ----------------------------ACLQISEGFSDMRYFLEEFLSKWRLLNGEIYV 240

Query: 241 LVGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENR 300
           LVGSRNID  EHCD HAQLTVDEYLQVVEVYLRT+IEIGL DVDLAVSWVE AALPEE R
Sbjct: 241 LVGSRNIDGIEHCDEHAQLTVDEYLQVVEVYLRTVIEIGLNDVDLAVSWVENAALPEEKR 300

Query: 301 QVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGG 360
           QVLLRRLDYL SLKAA  S+SS SSLLKD+ K HLSSSEE     ASKAA +PGYHQD G
Sbjct: 301 QVLLRRLDYLHSLKAASSSKSSPSSLLKDDQKQHLSSSEE---LHASKAALDPGYHQDRG 345

Query: 361 NATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTL 420
           NA RETVLRL+K+TKP FWPF +ITLKFGSTRLVIS +RI+L+CLSV+IYYLLRRKLTT+
Sbjct: 361 NANRETVLRLRKLTKPRFWPFRSITLKFGSTRLVISNKRIVLTCLSVVIYYLLRRKLTTM 345

Query: 421 KRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           KRIA +QASS+KKAL+DLWQLAFSYQVNPLAIAQPLS  ARGAS
Sbjct: 421 KRIAQKQASSVKKALLDLWQLAFSYQVNPLAIAQPLSDAARGAS 345

BLAST of Spg039022 vs. ExPASy Swiss-Prot
Match: Q8W4B2 (Protein APEM9 OS=Arabidopsis thaliana OX=3702 GN=APEM9 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.7e-44
Identity = 156/467 (33.40%), Postives = 210/467 (44.97%), Query Frame = 0

Query: 3   DAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYR--DKIGFE--------NDMLEAA 62
           +A  IW EI+RSESYLVCSMYEEA  L+SS+LKRI+   D +  E        +DMLE+A
Sbjct: 2   EATDIWGEIERSESYLVCSMYEEAESLSSSILKRIFGNIDVLSDEASQGDHQFHDMLESA 61

Query: 63  GMVLVQSLKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLT 122
           GMVLVQSL  +GRT  IV+EL+  F  VAAIPV VLLTG                     
Sbjct: 62  GMVLVQSLHGIGRTVEIVNELRDVFGEVAAIPVQVLLTG--------------------- 121

Query: 123 SLISLELPPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSP 182
                                                                       
Sbjct: 122 ------------------------------------------------------------ 181

Query: 183 DSHAASETSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEG-LTDMQCFLEEFLSKWN 242
                                               CLQIS G    ++  LEEF   W 
Sbjct: 182 -----------------------------------VCLQISNGSYLGVRDILEEFFRIW- 241

Query: 243 LLNKEIYVLVGSRNIDDREHCDGHAQ--LTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWV 302
            + K+ + ++    +  +     HA+  L +DEY++VVE+Y   ++     D+ LA+SWV
Sbjct: 242 -VYKDNHYILNDAGVSTKGF---HAKNCLDIDEYMEVVELYTFGVLAKFSNDMGLAISWV 301

Query: 303 EKAALPEENRQVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAA 362
           EKAALPEE RQ +LRRL  L SLK A    SS     KD   A +++          K+ 
Sbjct: 302 EKAALPEERRQGILRRLHSLLSLKTA----SSFEENSKDSSYAVVNN---------KKSL 328

Query: 363 GNPGYHQDGGNATRETVLRLQKVTKP-CFWPFHAITLKFGSTRLVISTRRIMLSCLSVLI 422
           GN        N   ++ L+L K  +P   W  H ++LK G+T+  +S  ++ +S + ++I
Sbjct: 362 GNE------KNDEIDSFLKLSKQHEPWSLWSSHPLSLKVGNTQFSMSRGKVAVSLVGLII 328

Query: 423 YYLLRRKLTTLKRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPL 456
            Y L+RK   L RI  RQ  S +KA+VD W+LAFSYQVNPLA  Q +
Sbjct: 422 CYALKRKRAALIRIIRRQMESTRKAIVDFWKLAFSYQVNPLAAIQSI 328

BLAST of Spg039022 vs. ExPASy TrEMBL
Match: A0A6J1J2X4 (protein APEM9 OS=Cucurbita maxima OX=3661 GN=LOC111482197 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.6e-141
Identity = 292/463 (63.07%), Postives = 318/463 (68.68%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSL 60
           MDD +AIWE+I+RSESYLVCSMYEEAL LASSVLKRI ++K G END+LEAAGMVLVQSL
Sbjct: 1   MDDGEAIWEDIERSESYLVCSMYEEALGLASSVLKRISQEKNGCENDLLEAAGMVLVQSL 60

Query: 61  KELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELP 120
           KELGRTSH+VDELKVSFASVA IPV VLLTG                             
Sbjct: 61  KELGRTSHMVDELKVSFASVAGIPVNVLLTG----------------------------- 120

Query: 121 PTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASET 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVL 240
                                      ACLQISEGL+DM+CFLEEFLSKW+LLN+EIYVL
Sbjct: 181 ---------------------------ACLQISEGLSDMRCFLEEFLSKWSLLNEEIYVL 240

Query: 241 VGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQ 300
            GSRNIDDREH D HAQLTVDEYLQVVEVYLRTL+EIGLKDVDLAVSWVEKAALPEE RQ
Sbjct: 241 AGSRNIDDREHYDDHAQLTVDEYLQVVEVYLRTLMEIGLKDVDLAVSWVEKAALPEEKRQ 300

Query: 301 VLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGN 360
           VLLRRLDY QSLKAA LSQSS SSL KD++KAHLSSSE+PQ SR SK A +PGYHQDGG+
Sbjct: 301 VLLRRLDYQQSLKAASLSQSSPSSLHKDDHKAHLSSSEDPQESRTSKTALDPGYHQDGGS 347

Query: 361 ATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLK 420
           A RETVL+L K+TKP FWPF  ITLKFGSTRLVISTR+++LSCL VLIYYLLRRKLTTLK
Sbjct: 361 ANRETVLKLHKLTKPWFWPFRTITLKFGSTRLVISTRKVVLSCLFVLIYYLLRRKLTTLK 347

Query: 421 RIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           R+A +Q  S+KKA+VDLWQLAFSYQVNPLAIAQPLSGV RGAS
Sbjct: 421 RMAQKQGLSVKKAVVDLWQLAFSYQVNPLAIAQPLSGVPRGAS 347

BLAST of Spg039022 vs. ExPASy TrEMBL
Match: A0A6J1FB87 (protein APEM9-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442442 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 2.0e-139
Identity = 289/463 (62.42%), Postives = 315/463 (68.03%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFENDMLEAAGMVLVQSL 60
           MDD +AIWE+I+RSESYLVCSMYEEAL LASSVLKRI ++  G END+LEAAGMVLVQSL
Sbjct: 1   MDDGEAIWEDIERSESYLVCSMYEEALGLASSVLKRISQENNGCENDLLEAAGMVLVQSL 60

Query: 61  KELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLELP 120
           KELGRTSH+VDELKVSF SV AIPV VLLTG                             
Sbjct: 61  KELGRTSHMVDELKVSFTSVTAIPVNVLLTG----------------------------- 120

Query: 121 PTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASET 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 SCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYVL 240
                                      ACLQISEGL+DM+CFLEEFLSKW+LLN+EIYVL
Sbjct: 181 ---------------------------ACLQISEGLSDMRCFLEEFLSKWSLLNEEIYVL 240

Query: 241 VGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENRQ 300
            G+RNIDDREH D HAQLTVDEYLQVVEVYL+TL+EIGLKDVDLAVSWVEKAALPEE RQ
Sbjct: 241 AGARNIDDREHYDDHAQLTVDEYLQVVEVYLQTLMEIGLKDVDLAVSWVEKAALPEEKRQ 300

Query: 301 VLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGGN 360
           VLLRRLDY QSLKAA LSQSS SSL KD++KAHLSSSE+ Q SRASK A +PGYH DGGN
Sbjct: 301 VLLRRLDYQQSLKAASLSQSSPSSLHKDDHKAHLSSSEDRQESRASKTALDPGYHPDGGN 347

Query: 361 ATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTLK 420
           A RETVL+L K+TKP FWPF  ITLKFGSTRL+ISTRR++LSCL VLIYYLLRRKLTTLK
Sbjct: 361 ANRETVLKLHKLTKPWFWPFRTITLKFGSTRLIISTRRVLLSCLFVLIYYLLRRKLTTLK 347

Query: 421 RIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           R+A +QA S KKA+VDLWQLAFSYQVNPLAIAQPLSGV RGAS
Sbjct: 421 RMAQKQALSAKKAMVDLWQLAFSYQVNPLAIAQPLSGVPRGAS 347

BLAST of Spg039022 vs. ExPASy TrEMBL
Match: A0A6J1CQT9 (protein APEM9 OS=Momordica charantia OX=3673 GN=LOC111013313 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.4e-132
Identity = 286/464 (61.64%), Postives = 309/464 (66.59%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFEN-DMLEAAGMVLVQS 60
           MDDA+AIWEEI+RSESYLVCSMYEEAL LA S+L RI +DK  +EN DMLEAAGMVLVQS
Sbjct: 1   MDDAKAIWEEIERSESYLVCSMYEEALSLAYSILTRISQDKSSWENGDMLEAAGMVLVQS 60

Query: 61  LKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLEL 120
           LKELGRTS IVDELK+SFASVAAIPV VLLTG                            
Sbjct: 61  LKELGRTSRIVDELKLSFASVAAIPVNVLLTG---------------------------- 120

Query: 121 PPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASE 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 TSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYV 240
                                       ACLQISEG +DM+ FLEEFLSKW LLN EIYV
Sbjct: 181 ----------------------------ACLQISEGFSDMRYFLEEFLSKWRLLNGEIYV 240

Query: 241 LVGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENR 300
           LVGSRNID  EHCD HAQLTVDEYLQVVEVYLRT+IEIGL DVDLAVSWVE AALPEE R
Sbjct: 241 LVGSRNIDGIEHCDEHAQLTVDEYLQVVEVYLRTVIEIGLNDVDLAVSWVENAALPEEKR 300

Query: 301 QVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGG 360
           QVLLRRLDYL SLKAA  S+SS SSLLKD+ K HLSSSEE     ASKAA +PGYHQD G
Sbjct: 301 QVLLRRLDYLHSLKAASSSKSSPSSLLKDDQKQHLSSSEE---LHASKAALDPGYHQDRG 345

Query: 361 NATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTL 420
           NA RETVLRL+K+TKP FWPF +ITLKFGSTRLVIS +RI+L+CLSV+IYYLLRRKLTT+
Sbjct: 361 NANRETVLRLRKLTKPRFWPFRSITLKFGSTRLVISNKRIVLTCLSVVIYYLLRRKLTTM 345

Query: 421 KRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           KRIA +QASS+KKAL+DLWQLAFSYQVNPLAIAQPLS  ARGAS
Sbjct: 421 KRIAQKQASSVKKALLDLWQLAFSYQVNPLAIAQPLSDAARGAS 345

BLAST of Spg039022 vs. ExPASy TrEMBL
Match: A0A5D3BP62 (3-phosphoinositide-dependent protein kinase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001350 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 3.3e-118
Identity = 256/464 (55.17%), Postives = 299/464 (64.44%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFE-NDMLEAAGMVLVQS 60
           MD  +AIW+EI+ +ESYLVC+M+EEA+ L+SSVLKRI + + G E N+M+E+AGMVL+QS
Sbjct: 1   MDAGEAIWKEIELAESYLVCAMFEEAVALSSSVLKRISQLENGIEKNEMMESAGMVLIQS 60

Query: 61  LKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLEL 120
           LKELGRTS I++EL+VSF+SVAAIP TVLL G                            
Sbjct: 61  LKELGRTSQILNELRVSFSSVAAIPFTVLLFG---------------------------- 120

Query: 121 PPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASE 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 TSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYV 240
                                       AC   SEGL+DMQ  LEEFL KWNLLN+E+YV
Sbjct: 181 ----------------------------ACFHFSEGLSDMQSLLEEFLGKWNLLNEEVYV 240

Query: 241 LVGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENR 300
            VGSR+IDDRE C  HAQLTVDEYLQ+V VYLR + EIGLKDVDLAVSWVEKAALPE  R
Sbjct: 241 FVGSRSIDDRE-C--HAQLTVDEYLQLVHVYLRIVTEIGLKDVDLAVSWVEKAALPEGKR 300

Query: 301 QVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGG 360
           Q+LLRRLDYLQS K A  SQSS+SSLL+++++ HLSSSE  Q+SRAS+ A +PGYHQDGG
Sbjct: 301 QILLRRLDYLQSKKPASSSQSSSSSLLRNDHRKHLSSSEGLQVSRASETALDPGYHQDGG 345

Query: 361 NATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTL 420
           +A RETVLRL K+TKP FWPF  ITLKFGS RLV+STR+I+LSC  VLIYYLLRRKLT +
Sbjct: 361 SANRETVLRLHKLTKPSFWPFRTITLKFGSFRLVVSTRKIVLSCFLVLIYYLLRRKLTAV 345

Query: 421 KRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           KR+A +Q SS+KKALVDLWQLAFSYQVNPLAIAQPLSG ARG S
Sbjct: 421 KRMAQKQGSSMKKALVDLWQLAFSYQVNPLAIAQPLSGAARGVS 345

BLAST of Spg039022 vs. ExPASy TrEMBL
Match: A0A1S3C5A0 (uncharacterized protein LOC103496818 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496818 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 3.3e-118
Identity = 256/464 (55.17%), Postives = 299/464 (64.44%), Query Frame = 0

Query: 1   MDDAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYRDKIGFE-NDMLEAAGMVLVQS 60
           MD  +AIW+EI+ +ESYLVC+M+EEA+ L+SSVLKRI + + G E N+M+E+AGMVL+QS
Sbjct: 1   MDAGEAIWKEIELAESYLVCAMFEEAVALSSSVLKRISQLENGIEKNEMMESAGMVLIQS 60

Query: 61  LKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLTSLISLEL 120
           LKELGRTS I++EL+VSF+SVAAIP TVLL G                            
Sbjct: 61  LKELGRTSQILNELRVSFSSVAAIPFTVLLFG---------------------------- 120

Query: 121 PPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSPDSHAASE 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 TSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEGLTDMQCFLEEFLSKWNLLNKEIYV 240
                                       AC   SEGL+DMQ  LEEFL KWNLLN+E+YV
Sbjct: 181 ----------------------------ACFHFSEGLSDMQSLLEEFLGKWNLLNEEVYV 240

Query: 241 LVGSRNIDDREHCDGHAQLTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWVEKAALPEENR 300
            VGSR+IDDRE C  HAQLTVDEYLQ+V VYLR + EIGLKDVDLAVSWVEKAALPE  R
Sbjct: 241 FVGSRSIDDRE-C--HAQLTVDEYLQLVHVYLRIVTEIGLKDVDLAVSWVEKAALPEGKR 300

Query: 301 QVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAAGNPGYHQDGG 360
           Q+LLRRLDYLQS K A  SQSS+SSLL+++++ HLSSSE  Q+SRAS+ A +PGYHQDGG
Sbjct: 301 QILLRRLDYLQSKKPASSSQSSSSSLLRNDHRKHLSSSEGLQVSRASETALDPGYHQDGG 345

Query: 361 NATRETVLRLQKVTKPCFWPFHAITLKFGSTRLVISTRRIMLSCLSVLIYYLLRRKLTTL 420
           +A RETVLRL K+TKP FWPF  ITLKFGS RLV+STR+I+LSC  VLIYYLLRRKLT +
Sbjct: 361 SANRETVLRLHKLTKPSFWPFRTITLKFGSFRLVVSTRKIVLSCFLVLIYYLLRRKLTAV 345

Query: 421 KRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPLSGVARGAS 464
           KR+A +Q SS+KKALVDLWQLAFSYQVNPLAIAQPLSG ARG S
Sbjct: 421 KRMAQKQGSSMKKALVDLWQLAFSYQVNPLAIAQPLSGAARGVS 345

BLAST of Spg039022 vs. TAIR 10
Match: AT3G10572.1 (3-phosphoinositide-dependent protein kinase-1, putative )

HSP 1 Score: 179.9 bits (455), Expect = 4.8e-45
Identity = 156/467 (33.40%), Postives = 210/467 (44.97%), Query Frame = 0

Query: 3   DAQAIWEEIDRSESYLVCSMYEEALELASSVLKRIYR--DKIGFE--------NDMLEAA 62
           +A  IW EI+RSESYLVCSMYEEA  L+SS+LKRI+   D +  E        +DMLE+A
Sbjct: 2   EATDIWGEIERSESYLVCSMYEEAESLSSSILKRIFGNIDVLSDEASQGDHQFHDMLESA 61

Query: 63  GMVLVQSLKELGRTSHIVDELKVSFASVAAIPVTVLLTGWELLENRLHSVACVLPSLDLT 122
           GMVLVQSL  +GRT  IV+EL+  F  VAAIPV VLLTG                     
Sbjct: 62  GMVLVQSLHGIGRTVEIVNELRDVFGEVAAIPVQVLLTG--------------------- 121

Query: 123 SLISLELPPTDKEAIDVAYLLYVFRDQFIHFGRRDDRVWSPDPSRSFSCHSYFQSLVLSP 182
                                                                       
Sbjct: 122 ------------------------------------------------------------ 181

Query: 183 DSHAASETSCRGVFGLRGIIRVLEEKRNRVRKFGRACLQISEG-LTDMQCFLEEFLSKWN 242
                                               CLQIS G    ++  LEEF   W 
Sbjct: 182 -----------------------------------VCLQISNGSYLGVRDILEEFFRIW- 241

Query: 243 LLNKEIYVLVGSRNIDDREHCDGHAQ--LTVDEYLQVVEVYLRTLIEIGLKDVDLAVSWV 302
            + K+ + ++    +  +     HA+  L +DEY++VVE+Y   ++     D+ LA+SWV
Sbjct: 242 -VYKDNHYILNDAGVSTKGF---HAKNCLDIDEYMEVVELYTFGVLAKFSNDMGLAISWV 301

Query: 303 EKAALPEENRQVLLRRLDYLQSLKAAGLSQSSTSSLLKDEYKAHLSSSEEPQISRASKAA 362
           EKAALPEE RQ +LRRL  L SLK A    SS     KD   A +++          K+ 
Sbjct: 302 EKAALPEERRQGILRRLHSLLSLKTA----SSFEENSKDSSYAVVNN---------KKSL 328

Query: 363 GNPGYHQDGGNATRETVLRLQKVTKP-CFWPFHAITLKFGSTRLVISTRRIMLSCLSVLI 422
           GN        N   ++ L+L K  +P   W  H ++LK G+T+  +S  ++ +S + ++I
Sbjct: 362 GNE------KNDEIDSFLKLSKQHEPWSLWSSHPLSLKVGNTQFSMSRGKVAVSLVGLII 328

Query: 423 YYLLRRKLTTLKRIATRQASSIKKALVDLWQLAFSYQVNPLAIAQPL 456
            Y L+RK   L RI  RQ  S +KA+VD W+LAFSYQVNPLA  Q +
Sbjct: 422 CYALKRKRAALIRIIRRQMESTRKAIVDFWKLAFSYQVNPLAAIQSI 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023526235.18.8e-14263.28protein APEM9-like [Cucurbita pepo subsp. pepo][more]
XP_022983646.13.3e-14163.07protein APEM9 [Cucurbita maxima][more]
XP_022935649.14.1e-13962.42protein APEM9-like isoform X1 [Cucurbita moschata] >XP_022935650.1 protein APEM9... [more]
KAG6581201.17.0e-13962.42Protein APEM9, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017940.1 Pro... [more]
XP_022143437.12.8e-13261.64protein APEM9 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q8W4B26.7e-4433.40Protein APEM9 OS=Arabidopsis thaliana OX=3702 GN=APEM9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J2X41.6e-14163.07protein APEM9 OS=Cucurbita maxima OX=3661 GN=LOC111482197 PE=4 SV=1[more]
A0A6J1FB872.0e-13962.42protein APEM9-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442442 PE=4... [more]
A0A6J1CQT91.4e-13261.64protein APEM9 OS=Momordica charantia OX=3673 GN=LOC111013313 PE=4 SV=1[more]
A0A5D3BP623.3e-11855.173-phosphoinositide-dependent protein kinase-1 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3C5A03.3e-11855.17uncharacterized protein LOC103496818 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT3G10572.14.8e-4533.403-phosphoinositide-dependent protein kinase-1, putative [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..360
IPR034571Protein APEM9PANTHERPTHR36361PROTEIN APEM9coord: 4..92
coord: 207..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039022.1Spg039022.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015919 peroxisomal membrane transport
cellular_component GO:0110165 cellular anatomical entity