Spg039016 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039016
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionbifunctional epoxide hydrolase 2-like
Locationscaffold12: 4066945 .. 4069693 (+)
RNA-Seq ExpressionSpg039016
SyntenySpg039016
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATGTATTGAAGTCAACCACGAGATTCACATTTTCACGAGGCGAAGATGATCTTACAAGATTCCCAGTGATCTGTTTATAATTCTTTGTAATGCCGAGAAACTGAGAAGAAGAAATTGAAAGTGAAACAGTGAGATCGAGAGCTGCCATGGATGCGATTGAACACAGCACCATCAACACCAATGGCATCAAAATGCACATCGCCTCCATAGGCACAGGCCCCGTCGTTCTTCTTCTCCATGGCTTCCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCTTCCGTCGGATATCGAGCCATCGCGCCCGACCTCCGCGGCTATGGCGACACCGACTCGCCCGAATCGCATACTTCCTACTCCGCGCTCCACATAGTCGGCGATCTGGTTGGGGCTTTGGATGAGCTTCGGATCGAGAAGGTGTTTCTGGTAGGGCATGATTGGGGAGCGATCATCGCGTGGTACTTTTGCTTGTTCAGGCCTGATAGAATCAAGGCGTTGGTGAATCTGAGTGTTCAGTTCTTCCCTAGGAATCCGGAAATTTCGTTCATCGAGCGTTTCAGAGCTGCCTACGGTGATGATTTCTATATGTGCAGGTTTCAGGTGAATCTGAACTTCAATTGATTTCTAAGAAGGAATTTGGTTTCTGTTTTTTCCCTTCCAATTCTGGTCCTAATTTCGATTCATCTCTGTGTATGCGATTTTGTTGCTTATAGTTGGATGAATGGTATAATCAATTTGCTTATTTATTCTCTCTCTCACATACACAATCAAAATGAATACTGTTGAAGATTGTTTTCTTATGTTGTTCCATTTAAACTTCTTTTGAGCTTTTAATTTGAGATCTCCAAGCTGTTAAATCTTACTATTTTTATCTTCTCCTCTGTGTAAGTAATTGCAAACTTTCCTGAGAAAGACTTTGTAGAAGTTGTAGTTGTGTACATTTGGAACATTCCTCCGCTAACAACTTCATAAGAAGAAGTGATTGAAATCCTTTTGACCTTTTTGGCGATTGTCTGTTGATTCATTCAAGGTTCTGGTTGGTGAGAGTCCAATCTTAACTTTGATGATTCTCTGGTTTAGTCAATAATTTTTTCTCAAAATTTGAGAGCGATATTCAACTTTGATGTGAATATATAAAGTCTTTCTGTTGAGCTAGAAAGTTTAAATTATAGCATCTTAGGCCCCGTTTTTGGTTTTTGGGTTTTGAAAACCAAATGGTTATCAAACGGGTCTTTAATGTTTTGGACCATTTCTTTTAACTAATAAAGCAAGTTCTGATATCATTTTTGTTGGAAACATAGGGGATGGCTCATGTTAATAGGAAAAGCACAAATGAATTGATTGTGGTTCTATGGCATCTTAATCTTAAACAACTAAGAGTAGATTAATTTCTAACAGGTTCCAGGAGAGGCAGAAGCAGACTTTGCCTCCATTGACACAGCTCAACTGTTCAAGACAACGCTATCTAATAGAAGTACACGGCCTCCATGCTTGCCTAAAGAGTTCGGATTTCGCGCGATTCCGCCTCCAGAAAACCTTCCTTCTTGGTTGACAGAAGAAGATATCAACTATTATGCTGCCGAATTTAAGAAGACAGGCTTCACTGGAGCATTGAACTACTATCGTGCTTTTGACTTGTAAGCCATGTTCATTTACATCCGAATTCGCTATTCATATTGTTGGGAGTGCGACCAAAGTTCCACATTGGCTAGATAAGGGGATGATCATGAGTTTATAAGGGAGGACAAGTATCTTCATTGGTACGAGACTTTTTGGAGTGAACCAAAAGCAAAGCCATGAGGATATCATACCATTGTGGAGATACGTGTGTGTCCGATAGTTCCTTAACGCATATCACTCTGATCGAACACTCTCTCTGTGTCATAAGTTCAACAATGGTTCCATGTTTCGAAATCGAATACATGTTTTCTTGATTCGGGCAAAACCTCGGTTTACCGTTCTAATTCTATTGCATTTTCATGGCAAGAAACTGCCCAATTGTCCCTGTTTCCTCACTTAGAAATGCATATTGTTAGCCATATCTCGTGGAAGGCAGCTGAGAGCATTGATGATTGATTTGGATGTATGAAGTTTAATTGATGTTTTGTTGCAGAACATGGGAGCTGACAGCGCCATGGACGGGAGCGAAGATTCAGGTACCCGTGAAGTTCATCGTCGGAGATACGGATATAACGTACCATTTTCAAGGAGCTAAGGAGTATATCCATAATGGCGGATTCAAAAGGGATGTGCCATTGCTGGAGGAAGTAGTTGTAGTAGAAGATGCTGGCCACTTTGTCCACGAAGAAAGGCCACATGAAATCAGTACTCACATCCATGACTTCATCCTGAAGTTTTGAACTCTATGAAAAAACTGGCTGCATACCGGTATGTTGGGATCTCCCTACATATCATCATTAAAATCTGGACAACTAGGAAAGAGAAAAAAGATTCTCATTTTCTCCCTTCCTCTTTTTTAGATGTCAAGATTTTAATGGAGTATGTAAAGAGATCCCTATATATCGATATGCAGTCAAGTCTTGTTCTTTTCATTGTTGCTATGTTGTGTTGGGAATTGAAACTTTTGATAATTTCAGCTGTTTATAATTTTGTTGTGTCTTTGAAAAGCAATTAGGAGCAGGGAAAGCCAAATTTGTTTCTGAAATATGGAATAATTTATGGGACTTAACAATGTAACACTTAATTTCTCTTTTATATATACATACACAATTCTCC

mRNA sequence

ATGGATGCGATTGAACACAGCACCATCAACACCAATGGCATCAAAATGCACATCGCCTCCATAGGCACAGGCCCCGTCGTTCTTCTTCTCCATGGCTTCCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCTTCCGTCGGATATCGAGCCATCGCGCCCGACCTCCGCGGCTATGGCGACACCGACTCGCCCGAATCGCATACTTCCTACTCCGCGCTCCACATAGTCGGCGATCTGGTTGGGGCTTTGGATGAGCTTCGGATCGAGAAGGTGTTTCTGGTAGGGCATGATTGGGGAGCGATCATCGCGTGGTACTTTTGCTTGTTCAGGCCTGATAGAATCAAGGCGTTGGTGAATCTGAGTGTTCAGTTCTTCCCTAGGAATCCGGAAATTTCGTTCATCGAGCGTTTCAGAGCTGCCTACGGTGATGATTTCTATATGTGCAGGTTTCAGGTTCCAGGAGAGGCAGAAGCAGACTTTGCCTCCATTGACACAGCTCAACTGTTCAAGACAACGCTATCTAATAGAAGTACACGGCCTCCATGCTTGCCTAAAGAGTTCGGATTTCGCGCGATTCCGCCTCCAGAAAACCTTCCTTCTTGGTTGACAGAAGAAGATATCAACTATTATGCTGCCGAATTTAAGAAGACAGGCTTCACTGGAGCATTGAACTACTATCGTGCTTTTGACTTAACATGGGAGCTGACAGCGCCATGGACGGGAGCGAAGATTCAGGTACCCGTGAAGTTCATCGTCGGAGATACGGATATAACGTACCATTTTCAAGGAGCTAAGGAGTATATCCATAATGGCGGATTCAAAAGGGATGTGCCATTGCTGGAGGAAGTAGTTGTAGTAGAAGATGCTGGCCACTTTGTCCACGAAGAAAGGCCACATGAAATCAGTACTCACATCCATGACTTCATCCTGAAGTTTTGA

Coding sequence (CDS)

ATGGATGCGATTGAACACAGCACCATCAACACCAATGGCATCAAAATGCACATCGCCTCCATAGGCACAGGCCCCGTCGTTCTTCTTCTCCATGGCTTCCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCTTCCGTCGGATATCGAGCCATCGCGCCCGACCTCCGCGGCTATGGCGACACCGACTCGCCCGAATCGCATACTTCCTACTCCGCGCTCCACATAGTCGGCGATCTGGTTGGGGCTTTGGATGAGCTTCGGATCGAGAAGGTGTTTCTGGTAGGGCATGATTGGGGAGCGATCATCGCGTGGTACTTTTGCTTGTTCAGGCCTGATAGAATCAAGGCGTTGGTGAATCTGAGTGTTCAGTTCTTCCCTAGGAATCCGGAAATTTCGTTCATCGAGCGTTTCAGAGCTGCCTACGGTGATGATTTCTATATGTGCAGGTTTCAGGTTCCAGGAGAGGCAGAAGCAGACTTTGCCTCCATTGACACAGCTCAACTGTTCAAGACAACGCTATCTAATAGAAGTACACGGCCTCCATGCTTGCCTAAAGAGTTCGGATTTCGCGCGATTCCGCCTCCAGAAAACCTTCCTTCTTGGTTGACAGAAGAAGATATCAACTATTATGCTGCCGAATTTAAGAAGACAGGCTTCACTGGAGCATTGAACTACTATCGTGCTTTTGACTTAACATGGGAGCTGACAGCGCCATGGACGGGAGCGAAGATTCAGGTACCCGTGAAGTTCATCGTCGGAGATACGGATATAACGTACCATTTTCAAGGAGCTAAGGAGTATATCCATAATGGCGGATTCAAAAGGGATGTGCCATTGCTGGAGGAAGTAGTTGTAGTAGAAGATGCTGGCCACTTTGTCCACGAAGAAAGGCCACATGAAATCAGTACTCACATCCATGACTTCATCCTGAAGTTTTGA

Protein sequence

MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNRSTRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELTAPWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEERPHEISTHIHDFILKF
Homology
BLAST of Spg039016 vs. NCBI nr
Match: XP_022984062.1 (uncharacterized protein LOC111482485 [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 7.5e-177
Identity = 293/316 (92.72%), Postives = 307/316 (97.15%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEH+T+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP+ISFIERFRA YGDDFYMCRFQVPGEAEADF  IDTAQLFK+TLSNR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPENLPSWLTEEDIN+YA++FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
            PWTGA+IQVPVKFIVGD DITYHF+GAKEYIHNGGFKRDVPLLEEVV+VEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           RPHEI+THIHDFI KF
Sbjct: 301 RPHEINTHIHDFINKF 316

BLAST of Spg039016 vs. NCBI nr
Match: XP_022934346.1 (uncharacterized protein LOC111441539 [Cucurbita moschata] >KAG6581279.1 hypothetical protein SDJN03_21281, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 628.6 bits (1620), Expect = 2.8e-176
Identity = 293/316 (92.72%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNPEISFIERFRA YGDDFYMCRFQVPGEAEADF  IDTAQLFK TLSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPE+LPSWLTEEDIN+YA++FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
            PWTGA+IQVPVKFIVGD DITYHF+GAKEYIHNGGFKRDVPLLEEVV+VEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           RPHEI+THIHDF  KF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of Spg039016 vs. NCBI nr
Match: XP_023528764.1 (uncharacterized protein LOC111791602 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 628.2 bits (1619), Expect = 3.7e-176
Identity = 293/316 (92.72%), Postives = 304/316 (96.20%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNPEISFIERFRA YGDDFYMCRFQVPGEAEADF  IDTAQLFK TLSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPENLPSWLTEEDIN+YA++FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
            PWTGA+IQVPVKFIVGD DITYHF+GAKEYIH GGFKRDVPLLEEVV+VEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHKGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           RPHEI+THIHDF  KF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of Spg039016 vs. NCBI nr
Match: XP_008454322.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044389.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29517.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 625.5 bits (1612), Expect = 2.4e-175
Identity = 289/316 (91.46%), Postives = 307/316 (97.15%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+H+TI TNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRAAYGDDFYMCRFQVPGEAEADFA IDTAQLFKTTLSNR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST+ PCLPKE+GFRAIPPPENLPSWLTEEDINYYAA+FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
           APWTG +IQVPVKFIVGD+D+TYHF+GAKEYIH GGFKRDVPLLEEVV+VE+AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           +PHEI+THIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Spg039016 vs. NCBI nr
Match: XP_004152242.1 (uncharacterized protein LOC101204329 [Cucumis sativus] >KGN52825.1 hypothetical protein Csa_014782 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 5.9e-174
Identity = 288/316 (91.14%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+H+TINTNGIKMHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP SHTSY+ALHIVGDL+GALDEL IEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRAAYGDDFYMCRFQVPGEAEADFA IDTAQLFKTTLS R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPENLPSWLTEEDINYYAA+FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
           APWTG +IQVPVKFIVGD DITYHF+GAKEYIH+GGFKRDVPLLEEVV+V++AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           +PHEI+THIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Spg039016 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.3e-58
Identity = 121/328 (36.89%), Postives = 183/328 (55.79%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGT--GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M A     ++TNG+++ +   G    PVV+L HGFPEL YSWRHQ+  L+  GY  +APD
Sbjct: 1   MGAPTERLVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPD 60

Query: 61  LRGYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRI 120
            RGYG +  PE+  +Y    +  DLVG LD++  E+   VGHDWGA++ W   L   DR+
Sbjct: 61  QRGYGGSSRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRV 120

Query: 121 KALVNLSVQFFPRNPEISFIERFRAAYGDD-FYMCRFQVPGEAEADFASIDTAQLFKTTL 180
            A+  LSV   PR  ++   + FR+ +G++ FY+  FQ PG A+A+    D A+  +  +
Sbjct: 121 AAVAALSVPALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNG-DPARTMRRMI 180

Query: 181 SNRSTRPP---------CLPKEFGF-RAIPPPENLPSWLTEEDINYYAAEFKKTGFTGAL 240
                RPP           P   GF   +P P  LP+W+++E++++Y  EF +TGFTG L
Sbjct: 181 G--GLRPPGDQSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGL 240

Query: 241 NYYRAFDLTWELTAPWTGAKIQVPVKFIVGDTD--ITYHFQGAKEYIHNGGFKRDVPLLE 300
           N+YR FD  WE TA   G  I VP  FI G  D  +T+        + +G ++       
Sbjct: 241 NWYRNFDRNWETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE------ 300

Query: 301 EVVVVEDAGHFVHEERPHEISTHIHDFI 314
             V+++ AGH++ +ERP E++  + +F+
Sbjct: 301 --VLIDGAGHWLQQERPGEVTAALLEFL 316

BLAST of Spg039016 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 101/304 (33.22%), Postives = 164/304 (53.95%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 72
           G+++H   +G+GP V L HGFPE W+SWR+Q+  L+  G+R +A D++GYG++ +P    
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRN 132
            YS   +  D+V  L++L + +   +GHDWG ++ W   LF P+R++A+ +L+  F P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFK--------TTLSNRSTRP 192
           P +S +E  +A    D Y   FQ PG AEA+    +  + FK        T L+    R 
Sbjct: 367 PNVSPMEIIKANPVFD-YQLYFQEPGVAEAELEQ-NLDRTFKNFFRAHDETFLTTNRVR- 426

Query: 193 PCLPKEFG--FRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELTAP 252
                E G  F   P   +L   +TEEDI +Y  +FKK+GF G LN+YR  +  W+    
Sbjct: 427 -----ELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCK 486

Query: 253 WTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEERP 307
            +G KI +P   +  + D+  H + +K        +  +P L+    ++D GH+   ++P
Sbjct: 487 GSGRKILIPALMVTAENDLVLHPKMSKH------MENWIPHLKR-GHIKDCGHWTQIDKP 535

BLAST of Spg039016 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 6.8e-48
Identity = 99/303 (32.67%), Postives = 161/303 (53.14%), Query Frame = 0

Query: 14  IKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHTS 73
           +++H   +G+GP V L HGFPE WYSWR+Q+  L+  GYR +A D++GYG++ +P     
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRNP 133
           Y    +  ++V  LD+L + +   +GHDWG ++ WY  LF P+R++A+ +L+  F P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 EISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTL--SNRSTRPPCLPKEF 193
            +S +E  +A    D Y   FQ PG AEA+    + ++ FK+    S+ S        E 
Sbjct: 368 NMSPLESIKANPVFD-YQLYFQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFRAIPPPE--NLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELTAPWTGAKIQ 253
           G   +  PE  +L   +TEE+I +Y  +FKK+GF G LN+YR  +  W+      G KI 
Sbjct: 428 GGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKIL 487

Query: 254 VPVKFIVGDTD------ITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEERPH 307
           +P   +  + D      ++ H +    ++  G              +ED GH+   ++P 
Sbjct: 488 IPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGH-------------IEDCGHWTQMDKPT 535

BLAST of Spg039016 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-47
Identity = 102/299 (34.11%), Postives = 167/299 (55.85%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRN 132
            Y+   +  ++V  LD+L I +   +GHDW  ++ W   LF P+R++A+ +L+  F P +
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTL-SNRSTRPPCLPK-- 192
           P++S ++  R+    + Y   FQ PG AEA+    + ++ FK+   ++  T    + K  
Sbjct: 365 PDVSPMKVIRSIPVFN-YQLYFQEPGVAEAELEK-NMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 EFGFRAIPPPE--NLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELTAPWTGAK 252
           E G   +  PE  NL    TEE+I +Y  +FKKTGF G LN+YR  +  W+ +    G K
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEERPHEIS 307
           I VP   +  + DI    + +K        ++ +P L+    +ED GH+   E+P E++
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGHWTQIEKPTEVN 534

BLAST of Spg039016 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.4e-47
Identity = 100/298 (33.56%), Postives = 159/298 (53.36%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRN 132
            Y+   +  ++V  L++L I +   +GHDW  ++ W   LF P+R++A+ +L+    P N
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PEISFIERFRAAYGDDFYMCRFQVPGEAEADFAS--IDTAQLFKTTLSNRSTRPPCLPKE 192
           PE+S +E  R+    + Y   FQ PG AEA+       T + F  T  +          E
Sbjct: 365 PEVSPMEVIRSIPVFN-YQLYFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATE 424

Query: 193 FGFRAIPPPEN--LPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELTAPWTGAKI 252
            G   +  PE+  +    TEE+I YY  +FKK+GF G LN+YR  +  W+ +    G KI
Sbjct: 425 MGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKI 484

Query: 253 QVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEERPHEIS 307
            VP   +  + DI    + +K        +  +P L+    +ED GH+   E+P E++
Sbjct: 485 LVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHWTQIEKPAEVN 534

BLAST of Spg039016 vs. ExPASy TrEMBL
Match: A0A6J1J139 (uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.6e-177
Identity = 293/316 (92.72%), Postives = 307/316 (97.15%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEH+T+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP+ISFIERFRA YGDDFYMCRFQVPGEAEADF  IDTAQLFK+TLSNR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPENLPSWLTEEDIN+YA++FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
            PWTGA+IQVPVKFIVGD DITYHF+GAKEYIHNGGFKRDVPLLEEVV+VEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           RPHEI+THIHDFI KF
Sbjct: 301 RPHEINTHIHDFINKF 316

BLAST of Spg039016 vs. ExPASy TrEMBL
Match: A0A6J1F1J8 (uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC111441539 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.4e-176
Identity = 293/316 (92.72%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNPEISFIERFRA YGDDFYMCRFQVPGEAEADF  IDTAQLFK TLSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPE+LPSWLTEEDIN+YA++FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
            PWTGA+IQVPVKFIVGD DITYHF+GAKEYIHNGGFKRDVPLLEEVV+VEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           RPHEI+THIHDF  KF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of Spg039016 vs. ExPASy TrEMBL
Match: A0A5D3E0A4 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001200 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 289/316 (91.46%), Postives = 307/316 (97.15%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+H+TI TNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRAAYGDDFYMCRFQVPGEAEADFA IDTAQLFKTTLSNR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST+ PCLPKE+GFRAIPPPENLPSWLTEEDINYYAA+FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
           APWTG +IQVPVKFIVGD+D+TYHF+GAKEYIH GGFKRDVPLLEEVV+VE+AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           +PHEI+THIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Spg039016 vs. ExPASy TrEMBL
Match: A0A1S3BZJ8 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494757 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 289/316 (91.46%), Postives = 307/316 (97.15%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+H+TI TNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSY+ALHIVGDLVGALDEL IEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRAAYGDDFYMCRFQVPGEAEADFA IDTAQLFKTTLSNR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST+ PCLPKE+GFRAIPPPENLPSWLTEEDINYYAA+FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
           APWTG +IQVPVKFIVGD+D+TYHF+GAKEYIH GGFKRDVPLLEEVV+VE+AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           +PHEI+THIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Spg039016 vs. ExPASy TrEMBL
Match: A0A0A0KT70 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001920 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.9e-174
Identity = 288/316 (91.14%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+H+TINTNGIKMHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP SHTSY+ALHIVGDL+GALDEL IEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRAAYGDDFYMCRFQVPGEAEADFA IDTAQLFKTTLS R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           ST  PCLPKE+GFRAIPPPENLPSWLTEEDINYYAA+FK+TGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
           APWTG +IQVPVKFIVGD DITYHF+GAKEYIH+GGFKRDVPLLEEVV+V++AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 RPHEISTHIHDFILKF 317
           +PHEI+THIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of Spg039016 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 463.4 bits (1191), Expect = 1.5e-130
Identity = 198/316 (62.66%), Postives = 254/316 (80.38%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TI+TNGI MH+ASIG+GPV+L +HGFP+LWYSWRHQL+  +++GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYSALHIVGDLVGALDELRIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGD+D+P S  SY+ LHIVGDLVG LD L +++VFLVGHDWGAI+AW+ C+ RPDR+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTTLSNR 180
           LVN SV F PRNP +  ++ FRA +GDD+Y+CRFQ PGE E DFA +DT +L     ++R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 STRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAFDLTWELT 240
           + RPPC+PK  GFR +P P +LP+WLTE+D+ +Y  +F + GFTG LNYYRA +L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 APWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDAGHFVHEE 300
           APWTG +I+VPVKFIVGD DITY+  G KEYIH GG K+ VP L+EVVV+E  GHF+H+E
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 RPHEISTHIHDFILKF 317
           +P E++ HI+ F  KF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of Spg039016 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 407.5 bits (1046), Expect = 9.6e-114
Identity = 186/323 (57.59%), Postives = 240/323 (74.30%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M+ I+H  ++ NGI MHIA  G   GPVVLLLHGFP+LWY+WRHQ+  LSS+GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDTDSPESHTSYSALHIVGDLVGALDEL--RIEKVFLVGHDWGAIIAWYFCLFRPD 120
           LRGYGD+DSPES + Y+ L++VGDLV  LD +    EKVFLVGHDWGAII W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKALVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTT 180
           +I   V LSV +  RNP++  ++ F+A +GDD+Y+CRFQ PG+ E + AS D     +  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LSNRSTRPPCLPKE--FGFRAIPPPEN--LPSWLTEEDINYYAAEFKKTGFTGALNYYRA 240
            + R+  PP LPK+  FG +  P  EN  LP W +++D+++Y ++F+K GFTG LNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 FDLTWELTAPWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVED 300
            DL WELTAPWTGAKIQVPVKF+ GD D+ Y   G KEYIH GGF  DVP L+E+VV+ED
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 AGHFVHEERPHEISTHIHDFILK 316
           AGHFV++E+P E++ HI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Spg039016 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 2.8e-105
Identity = 174/324 (53.70%), Postives = 230/324 (70.99%), Query Frame = 0

Query: 1   MDAIEHSTINTNGIKMHIA-----SIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 60
           +D +EH T+  NGI MH+A       G  P++L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDTDSPESHTSYSALHIVGDLVGALDELR--IEKVFLVGHDWGAIIAWYFCLF 120
           APDLRGYGDT++PE    Y+ L++ GD+V  +D +    + V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRIKALVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLF 180
           RP+++KALVN+SV F PRNP    +   R  +GDD+Y+CRFQ  GE E +F  + T  + 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KTTLSNRSTRPPCLPKEFGF-RAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRA 240
           K  L+ ++  P  LPK+  F R+      LP WLT+ED++YY  +++  GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FDLTWELTAPWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVED 300
            D  WELTAPWTGAKI+VPVKFI+GD D+TY+F GAKEYI+ GGFKRDVPLL+E VV++ 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AGHFVHEERPHEISTHIHDFILKF 317
            GHF+HEE P  I+ HIH+F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of Spg039016 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 365.9 bits (938), Expect = 3.2e-101
Identity = 174/325 (53.54%), Postives = 228/325 (70.15%), Query Frame = 0

Query: 4   IEHSTINTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           +EH  +  NGI +H+A  G   GP+VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDTDSPESHTSYSALHIVGDLVGALDELRI---EKVFLVGHDWGAIIAWYFCLFRPDRI 123
           YGD+D+P   +SY+  +IVGDL+  +  L     EKVF+VGHDWGA+IAWY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSV--QFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLFKTT 183
           KALVNLSV   F P +P +  ++R RA YGDD+Y+CRFQ  G+ EA+ A + T ++ K  
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LSNRSTRPPCLPKEFGF-----RAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYR 243
           L+ R+  P  +PK+  F       IP    LPSWLTEED+ Y+ ++F++ GF+G +NYYR
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFEEKGFSGPVNYYR 240

Query: 244 AFDLTWELTAPWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVE 303
            F+   EL  PW G+KIQVP KF++G+ D+ Y+  G KEYIH   FK DVPLLEE VV+E
Sbjct: 241 NFNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVME 300

Query: 304 DAGHFVHEERPHEISTHIHDFILKF 317
              HF+++E+P EI   I DFI KF
Sbjct: 301 GVAHFINQEKPQEILQIILDFISKF 321

BLAST of Spg039016 vs. TAIR 10
Match: AT4G15955.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 3.9e-99
Identity = 173/323 (53.56%), Postives = 222/323 (68.73%), Query Frame = 0

Query: 5   EHSTINTNGIKMHIAS-----IGTG----PVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 64
           +HS +  NGI MH+A       G G    PV+L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 6   DHSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTI 65

Query: 65  APDLRGYGDTDSPESHTSYSALHIVGDLVGALDEL--RIEKVFLVGHDWGAIIAWYFCLF 124
           APDLRGYGDTD+PES  +Y++LH+VGDL+G +D +    EKVF+VGHDWGAIIAW+ CLF
Sbjct: 66  APDLRGYGDTDAPESVDAYTSLHVVGDLIGLIDAVVGDREKVFVVGHDWGAIIAWHLCLF 125

Query: 125 RPDRIKALVNLSVQFFPRNPEISFIERFRAAYGDDFYMCRFQVPGEAEADFASIDTAQLF 184
           RPDR+KALVN+SV F P NP+      F+A YGDD+Y+CRFQ+               L 
Sbjct: 126 RPDRVKALVNMSVVFDPWNPKRKPTSTFKAFYGDDYYICRFQL------------LEILI 185

Query: 185 KTTLSNRSTRPPCLPKEFGFRAIPPPENLPSWLTEEDINYYAAEFKKTGFTGALNYYRAF 244
           K  +        C+      +      +LPSWLT+ D+ YY ++++K GFTG +NYYR  
Sbjct: 186 KIHV--------CIVG----KRYDDSVSLPSWLTDSDVKYYVSKYEKNGFTGPVNYYRNM 245

Query: 245 DLTWELTAPWTGAKIQVPVKFIVGDTDITYHFQGAKEYIHNGGFKRDVPLLEEVVVVEDA 304
           D TWEL    + AK++VPVKFI+GD D+TYH  G+K+YIH+G FK  VPLL+EVVV++  
Sbjct: 246 DRTWELMGSLSNAKVKVPVKFIIGDQDLTYHIPGSKKYIHDGRFKSHVPLLDEVVVIKGV 304

Query: 305 GHFVHEERPHEISTHIHDFILKF 317
           GHF+HEERP EIS HIHD+ L F
Sbjct: 306 GHFIHEERPDEISKHIHDYFLTF 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022984062.17.5e-17792.72uncharacterized protein LOC111482485 [Cucurbita maxima][more]
XP_022934346.12.8e-17692.72uncharacterized protein LOC111441539 [Cucurbita moschata] >KAG6581279.1 hypothet... [more]
XP_023528764.13.7e-17692.72uncharacterized protein LOC111791602 [Cucurbita pepo subsp. pepo][more]
XP_008454322.12.4e-17591.46PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044389.1 bi... [more]
XP_004152242.15.9e-17491.14uncharacterized protein LOC101204329 [Cucumis sativus] >KGN52825.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
I6YGS07.3e-5836.89Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C21.4e-4833.22Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349136.8e-4832.67Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349142.0e-4734.11Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802993.4e-4733.56Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1J1393.6e-17792.72uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485... [more]
A0A6J1F1J81.4e-17692.72uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
A0A5D3E0A41.2e-17591.46Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZJ81.2e-17591.46bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494757 PE... [more]
A0A0A0KT702.9e-17491.14AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
Match NameE-valueIdentityDescription
AT4G02340.11.5e-13062.66alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.19.6e-11457.59alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.12.8e-10553.70alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.13.2e-10153.54soluble epoxide hydrolase [more]
AT4G15955.33.9e-9953.56alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 111..124
score: 26.85
coord: 97..110
score: 33.0
coord: 51..66
score: 44.61
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..302
e-value: 1.7E-22
score: 80.3
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 51..66
score: 60.8
coord: 97..110
score: 52.92
coord: 290..312
score: 39.72
coord: 31..49
score: 55.26
coord: 111..124
score: 49.35
coord: 250..266
score: 35.56
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..316
e-value: 7.7E-108
score: 363.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..313
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..316
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039016.1Spg039016.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity