Spg038997 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg038997
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Alanine--tRNA ligase
Location	scaffold12: 4871524 .. 4875646 (+)
RNA-Seq Expression	Spg038997
Synteny	Spg038997

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACGAATGTCCATGGACATACAAGAATGAGAACAAATTGATAATTACAAAGTCCAAATAACGGACACCCATAAGAAAGCATTAAACCTCCCCACCTCCGAAACCTTTTCCAAAGAGATCTCAACCCTTCTAAAAGTTCTACCTCTCGATCCAAACTCCCCACAAATCTAGAGACAAATTATTTATTCTCTCCAAAATTGTGTAGGAAAGCCAATTTTTTATAGTAGCCGTCCTGGTAGATATTTGTCCTTAGTCCTCTTGTTAGTTTTGAATCAGAGGTTTTCCATTTAGGAGAATGAAAGGAAAAATAGTAGCCATGCTAATACAATTTTATATCCATTTCCTCAACCTTTATTCTCCAAAACCACATCTGGAAAATTTATGTTAACTAAATCAAAAGCTGCTCCATTTTTCTCGTATTAGCTAAGTTCTGTTTTTCTCATGGGAAGATGTTCTGGTCATTTTCAAATTTCAATCGTGTATTTGAAATTTTGATTGCTTGACTGCTCCTGAATTATAGGCAAGCCAGTGGATCCTGATGATTTGAGAAAAATAGAATCAATTGTTAACAAACAAATAGAAGATGAACTGGACGTAAATGCTAAGGAGGTAACCCTTGCTGATGCAAAGCGCATTAATGGGTTACGAGCTGTTTTTGGAGAAGTAAGATGCTAGTTGTCTTCGTTATTTCTGAAAGCTTTTATTTCATTTTCGACAAAAGCACGATCTCCAATATCTTCCTTATTTTATATGCAGGTCTATCCCGACCCAGTTAGAGTTGTTGCAATTGGGAAAAGTGTTGAGGAACTCCTGGCTGATCCAGATAATGAAAACTGGTTATCTATTTCTTCAGAGCTATGTGGAGGTCAGTGTGTTCTTGTCATGGCCTTCACTTTTTGCAGCCTGGGCCAAGTTTGCCTCAAATAAACCCAATGGATTATTGCAGGGACCCATATATCAAATACACGGGAAGCAAAGGCATTTGCCCTTCTATCTGAAGAAGGAATTGCCAAGGGCATTCGAAGAATTACTGCTGTTACCACTGATTCTGCCTTTAAGGCAATCGAGCAGGCTCAATCACTTGAGCAGGAAGTTGACGCTGCTTTTAATATAGATGGTAGCTTGCTGGAAAAGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACAATTTACAAACACTGGCAGTGGCAGATAGGTATCATCGGGGGAGGGGATGATTGTGGGAATTTTATGCTTGATAGGCTGATTTTTAACAGTTTACCTTTGCTTTTATATTTTAGTATTTCACCGTTGCATTACCCATTCCACTCATCCCTTTTCCTCTATTGGAAACTTCTAATAAACAAGAAAAGGATGCATGAGATTAGAGTTGGAAGGATATGAGTTATACTCAAAAGAAGAGAGATCAATACCCAGAAATGAAAAAAAGAGCCTCGTGTACAATAGGTCCTAGAAAAGGAAGAAAGGGTATAAAATTCTTTAGCATTTTTACATTAAGTTAATCTAAATAACTTAGGCCCCGTTTGATAACCATTTGGTTTTTTGTTTTTGGTTTTTGGTTTTTAAGCTTATAAACACCACTTCCAACTCTAAGTTTCTTTGGCTTTTCACCTACCATCTTTCCATGTTTTCAAAAACCAAGCCAATTTTTGAAAACTAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTAGAATTTGGTTAGGAATTCAAGTATGTCTCTAACAAAAATGAAAACCATGGTGAGGAAATTTAAAAAAAAACAAGCCTAATTTTCAAAAACTAAAAACAAAAAACGAAATGGTTATCAAACGGGGTCTTTAACTGTTTCAAAGACTCAAGATCATCAAACATGAAAATTTCTTGCACCCTGAAATCACTAAATTTCAGATAGTCCGTTGCTCTTTCTGTCTACCGCTCATCATTTAGTAGGATTTTTTTATTTTTTATTTTTAATTTTAAAATAAGAAACCAACTTTCAATGGTAGAACAAGGGCATACAAAAAACAATCCCCGGAATACAAACAAACTCACCAACCAAAGCTCCAGAAACTAGTTACAAAAACAAATGCCAATCTAAAAGAATCCACGGGTAATTACAAAAGGTCCGATTCACGGACGCCCATAAAGAAGAAAATATTAGGTTGATACACTGTACTCATCAATTGGATTAAAATTTCTCTTGCTGAATGTTGATTACATTGAACATGCCTCTAAAAGAATGATTTATGTTGAATTCTTGCTCGCTTCTATCCTGCAGGTGGTTGCTTCATTCAAAAACCGTGTTGATTCAGCACCAATTCCCGCTGCTAAAAAAGCACAAATTAGGTCTAAGATTGTCTTGCTCCAGGTATTGCTTGTAGACCCACTGTGCATTGATTACCCTCTTAAACTGTGTATTTATTGCCTGAAAACTGTTGTTTCATATAGAATGAAGTGAGGAAGGCTCAGAAGAAGCTTGCCGAAGAAAATATGCAGAAAGCTGTCAAAATTGCCATTGAAACAGCAGAAGTGGCTTCTTCAGATGGCAAGGCCTTCTGTATATCCCATGTCGATGTTGGTTTGGATGCAGCTGCTGTTCGTGAAGCAGTTGTCAAAGTTATGGAACAGAAGGTCATTTCTTATGCTTAGTAAAAAAACCATTTTGATGTTTGGATCTACATTTTCAAAAGTTGTTTTGCAAATGCATGAGAGTGGGAGTTTTCAAAAAATTTATGTCCCCACTGTATGCTTAAAAAAATTTATGACACCATTTGCCTCTCGCAATTTCCTTGATTTAGTGTGATTGTTTTACCATGGGCTCTTGAAGAAAATGGTCAATCTCCTCCCGTAGTGAAACATTTTGTTTATATCAAGAATTTCATTCTTGATTTCAAGTGAAGAAGTTTTCCAATTGGAGGTTGCATCAATAGGAAAATATCTTCTCATTGGAATGTGAGGATTTGATTTTATATTTGTGATCAACAGGGCATATCGGCGATGGTTTTCAGCACTGATGAAACCACAAAAAAGGCCGTGGTTTGTGCTGGGGTTCCTCAAAAAGGGAGTCTAGGGAAGCAATTAGAGGTGTCAGAGTGGTTGACAACAGCTTTGCAGCCTTTAAAAGGAAGGTGCGGGAAGGGGAAAGGCGGCCTTGCCACGGGCCAGGTAACTTTAATTACTTTGCTTCTCTATAGTATTTTTCTGGTTGAAGTTTCTCAACTCTAGTTCTCCAAATCTCATATACACTGACTTCTACCATATTCTCATGGTTGAGAATTAATTTATTGGGCTCATGACTAATATATATCTGAACAAAGTTGCATGATGAGGGAGATCAGGAATTTTGATACCCTTTGCTCCAGACTCAGATTCAGATCCTAATTCTAGAGAACAAAAGAAGTGCTTTTCTTTTTTCTCCTTTTTGGTGGTTATCGTTGGAACTTAGACTTGAATAATGGTGTTGGAATAGGAAGACTAAACCTTCATTTCGATCTTTTAATTGTAACATATTCATCTTCTGAAATGCTTTGTGCTTTTCAGGGAACTGATGCATCTCAAATCAAGGAGGCCATGGATCTTGCCACATCTTTTGCATCATTGAAACTGAACCAATGAAAGTTTTTAGGCTCCTGAAGTCTCACTTAGAGGAGGAGTTTTGCCATTATGTTCTGCATTAGTTGGGCTAACTTATGGAAAATGGGTATGGAATACTACACACACCTCAAGTTGAAGAATTTTCTGTCAAAATATTTGCTCCAGCTCTGCCTTCCACACTTGATTCATAGTTTTGGGTTGGGGGGTAATTATCTTTATATTCACTCACAAATACTGGTTGGATGTCCAAAGGAATCGATGCGATTCATACTACAATTTGAAGTACTAGATTTGTAAAGCATTGTCATTACTACATGCCATAGTGGCAATTTGAAAGTTGTGTTTGTTTTATTTTATTGTATTCTGTTCATGTGCTGTAGAAAATTAAATTAAATTATAGATTGTTGGTTCAACTGATGTGTAGCCTAAATCGATGTTGAATCAGATCTAAGGATTTGAGAAAAAAAAATAGAAAACTTTAGTCTTATCATTGATATATTCGTCTACTAAATCAATTTATTG

mRNA sequence

ATGAACGAATGCAAGCCAGTGGATCCTGATGATTTGAGAAAAATAGAATCAATTGTTAACAAACAAATAGAAGATGAACTGGACGTAAATGCTAAGGAGGTAACCCTTGCTGATGCAAAGCGCATTAATGGGTTACGAGCTGTTTTTGGAGAAGTCTATCCCGACCCAGTTAGAGTTGTTGCAATTGGGAAAAGTGTTGAGGAACTCCTGGCTGATCCAGATAATGAAAACTGGTTATCTATTTCTTCAGAGCTATGTGGAGGGACCCATATATCAAATACACGGGAAGCAAAGGCATTTGCCCTTCTATCTGAAGAAGGAATTGCCAAGGGCATTCGAAGAATTACTGCTGTGGTTGCTTCATTCAAAAACCGTGTTGATTCAGCACCAATTCCCGCTGCTAAAAAAGCACAAATTAGGTCTAAGATTGTCTTGCTCCAGAATGAAGTGAGGAAGGCTCAGAAGAAGCTTGCCGAAGAAAATATGCAGAAAGCTGTCAAAATTGCCATTGAAACAGCAGAAGTGGCTTCTTCAGATGGCAAGGCCTTCTGTATATCCCATGTCGATGTTGGTTTGGATGCAGCTGCTGTTCGTGAAGCAGTTGTCAAAGTTATGGAACAGAAGGGCATATCGGCGATGGTTTTCAGCACTGATGAAACCACAAAAAAGGCCGTGGTTTGTGCTGGGGTTCCTCAAAAAGGGAGTCTAGGGAAGCAATTAGAGGTGTCAGAGTGGTTGACAACAGCTTTGCAGCCTTTAAAAGGAAGGTGCGGGAAGGGGAAAGGCGGCCTTGCCACGGGCCAGGGAACTGATGCATCTCAAATCAAGGAGGCCATGGATCTTGCCACATCTTTTGCATCATTGAAACTGAACCAATGA

Coding sequence (CDS)

ATGAACGAATGCAAGCCAGTGGATCCTGATGATTTGAGAAAAATAGAATCAATTGTTAACAAACAAATAGAAGATGAACTGGACGTAAATGCTAAGGAGGTAACCCTTGCTGATGCAAAGCGCATTAATGGGTTACGAGCTGTTTTTGGAGAAGTCTATCCCGACCCAGTTAGAGTTGTTGCAATTGGGAAAAGTGTTGAGGAACTCCTGGCTGATCCAGATAATGAAAACTGGTTATCTATTTCTTCAGAGCTATGTGGAGGGACCCATATATCAAATACACGGGAAGCAAAGGCATTTGCCCTTCTATCTGAAGAAGGAATTGCCAAGGGCATTCGAAGAATTACTGCTGTGGTTGCTTCATTCAAAAACCGTGTTGATTCAGCACCAATTCCCGCTGCTAAAAAAGCACAAATTAGGTCTAAGATTGTCTTGCTCCAGAATGAAGTGAGGAAGGCTCAGAAGAAGCTTGCCGAAGAAAATATGCAGAAAGCTGTCAAAATTGCCATTGAAACAGCAGAAGTGGCTTCTTCAGATGGCAAGGCCTTCTGTATATCCCATGTCGATGTTGGTTTGGATGCAGCTGCTGTTCGTGAAGCAGTTGTCAAAGTTATGGAACAGAAGGGCATATCGGCGATGGTTTTCAGCACTGATGAAACCACAAAAAAGGCCGTGGTTTGTGCTGGGGTTCCTCAAAAAGGGAGTCTAGGGAAGCAATTAGAGGTGTCAGAGTGGTTGACAACAGCTTTGCAGCCTTTAAAAGGAAGGTGCGGGAAGGGGAAAGGCGGCCTTGCCACGGGCCAGGGAACTGATGCATCTCAAATCAAGGAGGCCATGGATCTTGCCACATCTTTTGCATCATTGAAACTGAACCAATGA

Protein sequence

MNECKPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGKSVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVRKAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGISAMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTDASQIKEAMDLATSFASLKLNQ

Homology

[Close]

BLAST of Spg038997 vs. NCBI nr
Match: XP_023527072.1 (alanine--tRNA ligase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-139
Identity = 277/321 (86.29%), Postives = 285/321 (88.79%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 715  KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 774

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
            ++EELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 775  NIEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 834

Query: 125  --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                      VVASFKNRVDSAPIPAAKKAQIR+KIVLLQNE+R
Sbjct: 835  KAFEQAQSLEQEVDAAFKIDGSLLEKVVASFKNRVDSAPIPAAKKAQIRAKIVLLQNELR 894

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKKLAEENMQKAVK+AIETAEV+SSDGKAFCISHV+VGLDAAAVREAVVKVMEQKGIS
Sbjct: 895  KAQKKLAEENMQKAVKVAIETAEVSSSDGKAFCISHVNVGLDAAAVREAVVKVMEQKGIS 954

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
            AMVFSTDETTKKAVVCAGVP KGS GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 955  AMVFSTDETTKKAVVCAGVPPKGSQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1014

[Close]

BLAST of Spg038997 vs. NCBI nr
Match: KAG7018049.1 (Alanine--tRNA ligase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 505.4 bits (1300), Expect = 3.4e-139
Identity = 276/321 (85.98%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 5   KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
           KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 614 KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 673

Query: 65  SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
           ++EELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 674 NIEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 733

Query: 125 --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                     VVASFKNRVDSAPIPAAKKAQIR+KIVLLQNE+R
Sbjct: 734 KAFEQAQSLEQEVDAAFKIDGSLLEKVVASFKNRVDSAPIPAAKKAQIRAKIVLLQNELR 793

Query: 185 KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
           KAQKKLAEENMQKAVK+AIETAEV+S DGKAFCISHV+VGLDAAAVREAVVKVMEQKGIS
Sbjct: 794 KAQKKLAEENMQKAVKVAIETAEVSSKDGKAFCISHVNVGLDAAAVREAVVKVMEQKGIS 853

Query: 245 AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
           AMVFSTDETTKKAVVCAGVP KGS GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 854 AMVFSTDETTKKAVVCAGVPPKGSQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 913

[Close]

BLAST of Spg038997 vs. NCBI nr
Match: KAA0052760.1 (alanine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 504.6 bits (1298), Expect = 5.7e-139
Identity = 275/321 (85.67%), Postives = 286/321 (89.10%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 710  KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 769

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
            +V+ELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 770  NVDELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 829

Query: 125  --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                      VVASFKNRVDSAPIPAA+KAQIR+KIVLLQNEVR
Sbjct: 830  KAIEQAQSLEQEVDAAFNIDGNLLEKVVASFKNRVDSAPIPAARKAQIRAKIVLLQNEVR 889

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKKLAEENMQKAVKIA+ETAE+ASS+GKAFCISHVDVGLDAAAVREAVV+VMEQKGIS
Sbjct: 890  KAQKKLAEENMQKAVKIAVETAEMASSEGKAFCISHVDVGLDAAAVREAVVRVMEQKGIS 949

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
            AMVFSTDETTKKAVVCAGVP KG+ GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 950  AMVFSTDETTKKAVVCAGVPPKGNQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1009

[Close]

BLAST of Spg038997 vs. NCBI nr
Match: TYK04196.1 (alanine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 504.6 bits (1298), Expect = 5.7e-139
Identity = 275/321 (85.67%), Postives = 286/321 (89.10%), Query Frame = 0


Query: 5   KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
           KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 636 KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 695

Query: 65  SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
           +V+ELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 696 NVDELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 755

Query: 125 --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                     VVASFKNRVDSAPIPAA+KAQIR+KIVLLQNEVR
Sbjct: 756 KAIEQAQSLEQEVDAAFNIDGNLLEKVVASFKNRVDSAPIPAARKAQIRAKIVLLQNEVR 815

Query: 185 KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
           KAQKKLAEENMQKAVKIA+ETAE+ASS+GKAFCISHVDVGLDAAAVREAVV+VMEQKGIS
Sbjct: 816 KAQKKLAEENMQKAVKIAVETAEMASSEGKAFCISHVDVGLDAAAVREAVVRVMEQKGIS 875

Query: 245 AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
           AMVFSTDETTKKAVVCAGVP KG+ GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 876 AMVFSTDETTKKAVVCAGVPPKGNQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 935

[Close]

BLAST of Spg038997 vs. NCBI nr
Match: XP_022934816.1 (alanine--tRNA ligase-like [Cucurbita moschata])

HSP 1 Score: 504.2 bits (1297), Expect = 7.5e-139
Identity = 275/321 (85.67%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 715  KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 774

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
            ++EELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 775  NIEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 834

Query: 125  --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                      VVASFKNRVDSAPIPAAKKAQIR+KIVLLQNE+R
Sbjct: 835  KAFEQAQSLEQEVDAAFKIDGSLLEKVVASFKNRVDSAPIPAAKKAQIRAKIVLLQNELR 894

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKKLAEENMQKAVK+AIETAEV+S DGKAFCISHV+VGLDAAAVREAVVKVMEQKGIS
Sbjct: 895  KAQKKLAEENMQKAVKVAIETAEVSSKDGKAFCISHVNVGLDAAAVREAVVKVMEQKGIS 954

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
            AMVFSTDETTKKAVVCAGVP +GS GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 955  AMVFSTDETTKKAVVCAGVPPRGSQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1014

[Close]

BLAST of Spg038997 vs. ExPASy Swiss-Prot
Match: P36428 (Alanine--tRNA ligase OS=Arabidopsis thaliana OX=3702 GN=ALATS PE=1 SV=3)

HSP 1 Score: 405.2 bits (1040), Expect = 6.2e-112
Identity = 217/320 (67.81%), Postives = 256/320 (80.00%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDP+DLR+IESIVNKQI+DELDV +KE  L++AKRI GLRAVFGEVYPDPVRVV+IG+
Sbjct: 684  KPVDPEDLRRIESIVNKQIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGR 743

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAV------ 124
             VE+LLADP+N  W  +SSE CGGTHI+NTREAKAFALLSEEGIAKGIRR+TAV      
Sbjct: 744  KVEDLLADPENNEWSLLSSEFCGGTHITNTREAKAFALLSEEGIAKGIRRVTAVTTECAF 803

Query: 125  ---------------------------VASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                       V++ K+RVD+A IPAAKKA IR+KI  LQNEVR
Sbjct: 804  DALNAASLLEREVEDASRAEGSALEKKVSALKSRVDAAIIPAAKKADIRTKIASLQNEVR 863

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKK+AE+N++K+VK+A E AE A+SDGK FCI  +DVGLDAAAVREAV KVME+KG+S
Sbjct: 864  KAQKKIAEQNLKKSVKLATEAAESAASDGKTFCIIQLDVGLDAAAVREAVSKVMEKKGMS 923

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 292
             MVFSTDE+T KAVVCAGVP+K    K L+V+EWLTTAL PLKGRCGKGKGGLA+GQGTD
Sbjct: 924  IMVFSTDESTNKAVVCAGVPEKSDQFKPLDVTEWLTTALGPLKGRCGKGKGGLASGQGTD 983

[Close]

BLAST of Spg038997 vs. ExPASy Swiss-Prot
Match: Q54Y20 (Alanine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=alaS PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.3e-40
Identity = 112/318 (35.22%), Postives = 169/318 (53.14%), Query Frame = 0


Query: 5   KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
           + +  D+L KI+ IVN QI  +LDV+AKEV LA AK+INGLRAVFGEVYPDPVRVV++G 
Sbjct: 629 RAITKDELIKIDQIVNDQIFKQLDVHAKEVGLASAKKINGLRAVFGEVYPDPVRVVSVGV 688

Query: 65  SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVVAS--- 124
            VE+L+ +P N  W + S E CGGTH+SNT++A+ F + SEE +  G+RRI AV  S   
Sbjct: 689 PVEQLIENPTNPEWANYSIEFCGGTHLSNTKQAELFTITSEETLGAGVRRIVAVTGSEAA 748

Query: 125 ------------FKNRVDSAPIPAAKK--------------AQIRSKIVLLQNEVRKAQK 184
                       F N +  +    AK+              A +R  +V    EV+  Q+
Sbjct: 749 SAIELNKELEVRFNNALKLSGSELAKEIVSLLDLLKVVTISASVRMNLVETLKEVQALQR 808

Query: 185 KLAEEN-----MQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGI 244
           K  +E       Q    +   + E+A S  K F +  V+   +   + E + K+  +  +
Sbjct: 809 KQVKEQETILAQQAQTYLEKTSEELAKSQPKVF-VDLVNFNSNTPLITETIKKIQTKSPM 868

Query: 245 SAMVFSTDETTKKAVVCAG-VPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQG 288
           +A++  + +  K  V C G VP+   + K L  + W+    + L G+ G GK  +A G G
Sbjct: 869 TAIMLISPDEEKGKVTCIGIVPKDSEISKTLTANAWVVKVTEVLGGK-GGGKVDVAQGVG 928

[Close]

BLAST of Spg038997 vs. ExPASy Swiss-Prot
Match: Q5A8K2 (Alanine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ALA1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-34
Identity = 107/317 (33.75%), Postives = 159/317 (50.16%), Query Frame = 0


Query: 7   VDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGKSV 66
           V   +L KIE+I NK I++   V  K+V+L  AK INGLRAVFGE YPDPVRVV+IG SV
Sbjct: 656 VTAKELEKIEAISNKYIKNNDKVYYKDVSLTKAKEINGLRAVFGETYPDPVRVVSIGVSV 715

Query: 67  EELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVVA------ 126
           ++LLADP N  W  IS E CGGTH++ T + K   ++ E GIAKGIRRI AV        
Sbjct: 716 DDLLADPTNTKWHEISIEFCGGTHVAKTGDIKDLVIIEESGIAKGIRRIVAVTGHDAHHV 775

Query: 127 -----SFKNRVDSA---PIPAAKKAQIRSKIVLLQ-------------NEVRKAQKKLAE 186
                 F+  +D+A   P   AK+++ +   V L+              +  K  K + +
Sbjct: 776 QKVANEFEQEIDNASSLPFGVAKESKSKELGVALKKLSISVLDKQRLTEKFNKLDKSIKD 835

Query: 187 ENMQKAVKIAIETAEVASS------DGKAFCISHVDVGLDAAAVREAVVKVMEQKGISAM 246
               K  +   +T +V ++      +  +F ++HV +  +A A+ EA+  + +Q    ++
Sbjct: 836 NLKAKQKEETKKTLDVVNNWLNDKENASSFLVAHVPITANAKAITEAINLIKKQDKTKSI 895

Query: 247 VFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTDAS 291
              T ET K A  C       ++ K +  +E      + + G+ G GKG +  G G    
Sbjct: 896 YLLTGETDKVAHGC--YVSDEAIAKGINANELAKAVSENIGGKAG-GKGNIVQGMGDKPQ 955

[Close]

BLAST of Spg038997 vs. ExPASy Swiss-Prot
Match: P40825 (Alanine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ALA1 PE=1 SV=3)

HSP 1 Score: 142.5 bits (358), Expect = 7.5e-33
Identity = 107/323 (33.13%), Postives = 166/323 (51.39%), Query Frame = 0


Query: 5   KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
           K V  ++L+K+E I N+QI++ L V  KE+ L  AK I+G+RAVFGE YPDPVRVV++GK
Sbjct: 663 KAVSNEELKKVEDICNEQIKENLQVFYKEIPLDLAKSIDGVRAVFGETYPDPVRVVSVGK 722

Query: 65  SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVVAS--- 124
            +EELLA+P NE W   S E CGGTH++ T + K F +L E GIAKGIRRI AV  +   
Sbjct: 723 PIEELLANPANEEWTKYSIEFCGGTHVNKTGDIKYFVILEESGIAKGIRRIVAVTGTEAF 782

Query: 125 --------FKNRVDSA------PIPAAKKAQIRSKI------VLLQNEVR----KAQKKL 184
                   F   +D+A      PI   K  ++  K+      V+ +NE++    K +K +
Sbjct: 783 EAQRLAEQFAADLDAADKLPFSPIKEKKLKELGVKLGQLSISVITKNELKQKFNKIEKAV 842

Query: 185 AEENMQKAVKIAIETAE-----VASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS- 244
            +E   +A K   +T +       +++   + +  +D+  +A A+ EA+  +     +  
Sbjct: 843 KDEVKSRAKKENKQTLDEVKTFFETNENAPYLVKFIDISPNAKAITEAINYMKSNDSVKD 902

Query: 245 ---AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQ 292
               ++   D   + A  C       +L K ++ S         + G+ G GKG +  G 
Sbjct: 903 KSIYLLAGNDPEGRVAHGC--YISNAALAKGIDGSALAKKVSSIIGGKAG-GKGNVFQGM 962

[Close]

BLAST of Spg038997 vs. ExPASy Swiss-Prot
Match: O13914 (Alanine--tRNA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ala1 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-31
Identity = 107/313 (34.19%), Postives = 153/313 (48.88%), Query Frame = 0


Query: 10  DDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGKSVEEL 69
           D L  +E+  N  I+D L V +KEV L+ AK INGLRAVFGEVYPDPVRVV IG  ++ L
Sbjct: 649 DKLLLVENYCNNVIQDNLSVYSKEVALSKAKEINGLRAVFGEVYPDPVRVVCIGVDIDTL 708

Query: 70  LADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVVASFKNRV--- 129
           L +P   +W   S E CGGTH   + E K F +L E GIAKGIRRI AV ++  NRV   
Sbjct: 709 LQEPKKPDWTKYSIEFCGGTHCDKSGEIKDFVILEENGIAKGIRRIVAVTSTEANRVSRL 768

Query: 130 -----------DSAPIPAAKKAQIR------SKIVLLQNEVRKAQKKLAE--ENMQKAVK 189
                      +  P   AK+A+++      SK+V+        ++++A+  + +Q+ VK
Sbjct: 769 ANEFDARIAKLEKMPFSPAKEAELKKISVDLSKLVVAAVRKHAMKERIAKITKQVQEHVK 828

Query: 190 I--AIETAEVAS--------SDGKAFCISHVDVGLDAAAVREAVVKVMEQKGISAMVFST 249
              A E  EV +        +   +F ++ V +  +  A+  A+    +     ++    
Sbjct: 829 AINAAEQKEVVNVVTEYFKENPDMSFVVAKVPISANPKALSFALTYAKKNLKDKSIYLLA 888

Query: 250 DETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTDASQIKE 291
            + TK A  C   P+     K+L   EW       + GR G GKG    G G     I  
Sbjct: 889 SDDTKVAHACLVSPE---AMKKLTPQEWSQKVCHSIGGRSG-GKGDTCQGVGDKPLSIDV 948

[Close]

BLAST of Spg038997 vs. ExPASy TrEMBL
Match: A0A5D3C1B3 (Alanine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00220 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.8e-139
Identity = 275/321 (85.67%), Postives = 286/321 (89.10%), Query Frame = 0


Query: 5   KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
           KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 636 KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 695

Query: 65  SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
           +V+ELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 696 NVDELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 755

Query: 125 --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                     VVASFKNRVDSAPIPAA+KAQIR+KIVLLQNEVR
Sbjct: 756 KAIEQAQSLEQEVDAAFNIDGNLLEKVVASFKNRVDSAPIPAARKAQIRAKIVLLQNEVR 815

Query: 185 KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
           KAQKKLAEENMQKAVKIA+ETAE+ASS+GKAFCISHVDVGLDAAAVREAVV+VMEQKGIS
Sbjct: 816 KAQKKLAEENMQKAVKIAVETAEMASSEGKAFCISHVDVGLDAAAVREAVVRVMEQKGIS 875

Query: 245 AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
           AMVFSTDETTKKAVVCAGVP KG+ GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 876 AMVFSTDETTKKAVVCAGVPPKGNQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 935

[Close]

BLAST of Spg038997 vs. ExPASy TrEMBL
Match: A0A5A7UC57 (Alanine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43055G00130 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.8e-139
Identity = 275/321 (85.67%), Postives = 286/321 (89.10%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 710  KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 769

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
            +V+ELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 770  NVDELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 829

Query: 125  --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                      VVASFKNRVDSAPIPAA+KAQIR+KIVLLQNEVR
Sbjct: 830  KAIEQAQSLEQEVDAAFNIDGNLLEKVVASFKNRVDSAPIPAARKAQIRAKIVLLQNEVR 889

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKKLAEENMQKAVKIA+ETAE+ASS+GKAFCISHVDVGLDAAAVREAVV+VMEQKGIS
Sbjct: 890  KAQKKLAEENMQKAVKIAVETAEMASSEGKAFCISHVDVGLDAAAVREAVVRVMEQKGIS 949

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
            AMVFSTDETTKKAVVCAGVP KG+ GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 950  AMVFSTDETTKKAVVCAGVPPKGNQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1009

[Close]

BLAST of Spg038997 vs. ExPASy TrEMBL
Match: A0A6J1F2W2 (Alanine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111441875 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.6e-139
Identity = 275/321 (85.67%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 715  KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 774

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
            ++EELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 775  NIEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 834

Query: 125  --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                      VVASFKNRVDSAPIPAAKKAQIR+KIVLLQNE+R
Sbjct: 835  KAFEQAQSLEQEVDAAFKIDGSLLEKVVASFKNRVDSAPIPAAKKAQIRAKIVLLQNELR 894

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKKLAEENMQKAVK+AIETAEV+S DGKAFCISHV+VGLDAAAVREAVVKVMEQKGIS
Sbjct: 895  KAQKKLAEENMQKAVKVAIETAEVSSKDGKAFCISHVNVGLDAAAVREAVVKVMEQKGIS 954

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
            AMVFSTDETTKKAVVCAGVP +GS GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 955  AMVFSTDETTKKAVVCAGVPPRGSQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1014

[Close]

BLAST of Spg038997 vs. ExPASy TrEMBL
Match: A0A6J1J7W9 (Alanine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111482084 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 274/321 (85.36%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 715  KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 774

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA------- 124
            ++EELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITA       
Sbjct: 775  NIEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 834

Query: 125  --------------------------VVASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                      VVASFKNRVDSAPIPAA+KAQIR+KIVLLQNE+R
Sbjct: 835  KAFKQAQSLEQEVDAAFNIDGSLLEKVVASFKNRVDSAPIPAARKAQIRAKIVLLQNELR 894

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKK+AEENMQKAVK+AIETAEV+SSDGKAFCISHV+VGLDAAAVREAVVKVMEQKGI 
Sbjct: 895  KAQKKVAEENMQKAVKVAIETAEVSSSDGKAFCISHVNVGLDAAAVREAVVKVMEQKGIP 954

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
            AMVFSTDETTKKAVVCAGVP KGS GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 955  AMVFSTDETTKKAVVCAGVPPKGSQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1014

[Close]

BLAST of Spg038997 vs. ExPASy TrEMBL
Match: A0A0A0LEP6 (Alanine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G656490 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.3e-138
Identity = 273/321 (85.05%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDPDDLRKIESIVNKQIEDELDVNA+EVTLA+AKRINGLRAVFGEVYPDPVRVVAIGK
Sbjct: 710  KPVDPDDLRKIESIVNKQIEDELDVNAQEVTLAEAKRINGLRAVFGEVYPDPVRVVAIGK 769

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAV------ 124
            +V+ELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAV      
Sbjct: 770  NVDELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVTTDSAF 829

Query: 125  ---------------------------VASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                       VASFKNRVDSAPIPAA+KAQIR+KIVLLQNEVR
Sbjct: 830  KAIEQAQSLEQEVDAAFNIDGNLLEKMVASFKNRVDSAPIPAARKAQIRTKIVLLQNEVR 889

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKKLAEENMQKAVKIA+ETAE+ASS+GK FCISHVDVGLDAAAVREAVV+VMEQKGIS
Sbjct: 890  KAQKKLAEENMQKAVKIAVETAEMASSEGKPFCISHVDVGLDAAAVREAVVRVMEQKGIS 949

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 293
             MVFSTDETTKKAVVCAGVP KG+ GKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD
Sbjct: 950  TMVFSTDETTKKAVVCAGVPPKGNQGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 1009

[Close]

BLAST of Spg038997 vs. TAIR 10
Match: AT1G50200.1 (Alanyl-tRNA synthetase )

HSP 1 Score: 405.2 bits (1040), Expect = 4.4e-113
Identity = 217/320 (67.81%), Postives = 256/320 (80.00%), Query Frame = 0


Query: 5    KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
            KPVDP+DLR+IESIVNKQI+DELDV +KE  L++AKRI GLRAVFGEVYPDPVRVV+IG+
Sbjct: 684  KPVDPEDLRRIESIVNKQIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGR 743

Query: 65   SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAV------ 124
             VE+LLADP+N  W  +SSE CGGTHI+NTREAKAFALLSEEGIAKGIRR+TAV      
Sbjct: 744  KVEDLLADPENNEWSLLSSEFCGGTHITNTREAKAFALLSEEGIAKGIRRVTAVTTECAF 803

Query: 125  ---------------------------VASFKNRVDSAPIPAAKKAQIRSKIVLLQNEVR 184
                                       V++ K+RVD+A IPAAKKA IR+KI  LQNEVR
Sbjct: 804  DALNAASLLEREVEDASRAEGSALEKKVSALKSRVDAAIIPAAKKADIRTKIASLQNEVR 863

Query: 185  KAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVKVMEQKGIS 244
            KAQKK+AE+N++K+VK+A E AE A+SDGK FCI  +DVGLDAAAVREAV KVME+KG+S
Sbjct: 864  KAQKKIAEQNLKKSVKLATEAAESAASDGKTFCIIQLDVGLDAAAVREAVSKVMEKKGMS 923

Query: 245  AMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGGLATGQGTD 292
             MVFSTDE+T KAVVCAGVP+K    K L+V+EWLTTAL PLKGRCGKGKGGLA+GQGTD
Sbjct: 924  IMVFSTDESTNKAVVCAGVPEKSDQFKPLDVTEWLTTALGPLKGRCGKGKGGLASGQGTD 983

[Close]

BLAST of Spg038997 vs. TAIR 10
Match: AT1G50200.2 (Alanyl-tRNA synthetase )

HSP 1 Score: 397.9 bits (1021), Expect = 7.0e-111
Identity = 217/328 (66.16%), Postives = 256/328 (78.05%), Query Frame = 0


Query: 5   KPVDPDDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPDPVRVVAIGK 64
           KPVDP+DLR+IESIVNKQI+DELDV +KE  L++AKRI GLRAVFGEVYPDPVRVV+IG+
Sbjct: 636 KPVDPEDLRRIESIVNKQIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGR 695

Query: 65  SVEELLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAV------ 124
            VE+LLADP+N  W  +SSE CGGTHI+NTREAKAFALLSEEGIAKGIRR+TAV      
Sbjct: 696 KVEDLLADPENNEWSLLSSEFCGGTHITNTREAKAFALLSEEGIAKGIRRVTAVTTECAF 755

Query: 125 ---------------------------VASFKNRVDSAPIPAAKKAQIRSKIVLLQ---- 184
                                      V++ K+RVD+A IPAAKKA IR+KI  LQ    
Sbjct: 756 DALNAASLLEREVEDASRAEGSALEKKVSALKSRVDAAIIPAAKKADIRTKIASLQVISS 815

Query: 185 ----NEVRKAQKKLAEENMQKAVKIAIETAEVASSDGKAFCISHVDVGLDAAAVREAVVK 244
               NEVRKAQKK+AE+N++K+VK+A E AE A+SDGK FCI  +DVGLDAAAVREAV K
Sbjct: 816 FSLTNEVRKAQKKIAEQNLKKSVKLATEAAESAASDGKTFCIIQLDVGLDAAAVREAVSK 875

Query: 245 VMEQKGISAMVFSTDETTKKAVVCAGVPQKGSLGKQLEVSEWLTTALQPLKGRCGKGKGG 292
           VME+KG+S MVFSTDE+T KAVVCAGVP+K    K L+V+EWLTTAL PLKGRCGKGKGG
Sbjct: 876 VMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQFKPLDVTEWLTTALGPLKGRCGKGKGG 935

[Close]

BLAST of Spg038997 vs. TAIR 10
Match: AT5G22800.1 (Alanyl-tRNA synthetase, class IIc )

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-15
Identity = 86/292 (29.45%), Postives = 135/292 (46.23%), Query Frame = 0


Query: 10  DDLRKIESIVNKQIEDELDVNAKEVTLADAKRINGLRAVFGEVYPD-PVRVVAIGKSVEE 69
           ++L +IE ++N+ I D   +  K + LADAKR  G  A+FGE Y +  VRVV +      
Sbjct: 698 NELEEIECLINRWIGDATRLETKVLPLADAKRA-GAIAMFGEKYDENEVRVVEVP----- 757

Query: 70  LLADPDNENWLSISSELCGGTHISNTREAKAFALLSEEGIAKGIRRITAVVA----SFKN 129
                       +S ELCGGTH+ NT E +AF ++SE+GIA GIRRI AV       + N
Sbjct: 758 -----------GVSMELCGGTHVGNTAEIRAFKIISEQGIASGIRRIEAVAGEAFIEYIN 817

Query: 130 RVDS-----APIPAAKKAQIRSKIVLLQNEVRKAQKKLAEENMQKAVKIAIETAEVASSD 189
             DS           K   + +++  L  E+R A+K+ ++   + AV  A   +  A + 
Sbjct: 818 SRDSQMTRLCSTLKVKAEDVTNRVENLLEELRAARKEASDLRSKAAVYKASVISNKAFTV 877

Query: 190 GKAFCISHVDVGLD------AAAVREAVVKVMEQKGISAMVFSTDETTKKAVVCAGVPQK 249
           G +  I  +   +D        +  E ++  +E   ++ ++ S+ E  K ++V A  P  
Sbjct: 878 GTSQTIRVLVESMDDTDADSLKSAAEHLISTLEDP-VAVVLGSSPEKDKVSLVAAFSPGV 937

Query: 250 GSLGKQLEVSEWLTTALQPLKGRCGKGKGG---LATGQGTDASQIKEAMDLA 283
            SLG Q          + P+   CG G GG    A   G     +  A++ A
Sbjct: 938 VSLGVQ------AGKFIGPIAKLCGGGGGGKPNFAQAGGRKPENLPSALEKA 965

The following BLAST results are available for this feature:

BLAST of Spg038997 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527072.1	1.2e-139	86.29	alanine--tRNA ligase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7018049.1	3.4e-139	85.98	Alanine--tRNA ligase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAA0052760.1	5.7e-139	85.67	alanine--tRNA ligase [Cucumis melo var. makuwa]	[more]
TYK04196.1	5.7e-139	85.67	alanine--tRNA ligase [Cucumis melo var. makuwa]	[more]
XP_022934816.1	7.5e-139	85.67	alanine--tRNA ligase-like [Cucurbita moschata]	[more]

BLAST of Spg038997 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36428	6.2e-112	67.81	Alanine--tRNA ligase OS=Arabidopsis thaliana OX=3702 GN=ALATS PE=1 SV=3	[more]
Q54Y20	8.3e-40	35.22	Alanine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=alaS P...	[more]
Q5A8K2	1.4e-34	33.75	Alanine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=2375...	[more]
P40825	7.5e-33	33.13	Alanine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204...	[more]
O13914	1.4e-31	34.19	Alanine--tRNA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=2...	[more]

BLAST of Spg038997 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C1B3	2.8e-139	85.67	Alanine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold185...	[more]
A0A5A7UC57	2.8e-139	85.67	Alanine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430...	[more]
A0A6J1F2W2	3.6e-139	85.67	Alanine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111441875 PE=3 SV=1	[more]
A0A6J1J7W9	1.1e-138	85.36	Alanine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111482084 PE=3 SV=1	[more]
A0A0A0LEP6	2.3e-138	85.05	Alanine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G656490 PE=3 SV=1	[more]

BLAST of Spg038997 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50200.1	4.4e-113	67.81	Alanyl-tRNA synthetase	[more]
AT1G50200.2	7.0e-111	66.16	Alanyl-tRNA synthetase	[more]
AT5G22800.1	1.9e-15	29.45	Alanyl-tRNA synthetase, class IIc	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 136..163
None	No IPR available	GENE3D	3.10.310.40		coord: 177..286 e-value: 5.7E-10 score: 41.2
None	No IPR available	GENE3D	3.30.980.10		coord: 2..129 e-value: 1.2E-25 score: 92.3
None	No IPR available	PANTHER	PTHR11777	ALANYL-TRNA SYNTHETASE	coord: 3..120
None	No IPR available	PANTHER	PTHR11777:SF35	BNACNNG61840D PROTEIN	coord: 3..120
None	No IPR available	PANTHER	PTHR11777	ALANYL-TRNA SYNTHETASE	coord: 119..291
None	No IPR available	PANTHER	PTHR11777:SF35	BNACNNG61840D PROTEIN	coord: 119..291
IPR012947	Threonyl/alanyl tRNA synthetase, SAD	SMART	SM00863	tRNA_SAD_4	coord: 57..116 e-value: 2.3E-20 score: 83.7
IPR012947	Threonyl/alanyl tRNA synthetase, SAD	PFAM	PF07973	tRNA_SAD	coord: 57..115 e-value: 6.4E-18 score: 64.6
IPR003156	DHHA1 domain	PFAM	PF02272	DHHA1	coord: 146..284 e-value: 1.2E-13 score: 51.6
IPR018165	Alanyl-tRNA synthetase, class IIc, core domain	PROSITE	PS50860	AA_TRNA_LIGASE_II_ALA	coord: 1..120 score: 16.199976
IPR018163	Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily	SUPERFAMILY	55186	ThrRS/AlaRS common domain	coord: 5..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg038997.1	Spg038997.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106074	aminoacyl-tRNA metabolism involved in translational fidelity
biological_process	GO:0070143	mitochondrial alanyl-tRNA aminoacylation
biological_process	GO:0006400	tRNA modification
biological_process	GO:0006419	alanyl-tRNA aminoacylation
biological_process	GO:0043039	tRNA aminoacylation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004813	alanine-tRNA ligase activity
molecular_function	GO:0016597	amino acid binding
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding