Spg038953 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg038953
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	transmembrane ascorbate ferrireductase 1
Location	scaffold12: 463161 .. 467909 (-)
RNA-Seq Expression	Spg038953
Synteny	Spg038953

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTATCGGTGAAGGCTTTTCCGTTCACTGTGGTGAGTCACGTGCTCGCAATCGTGGCGTTGATTTTGGTGCTCATTTGGACCATTCACTTCAGAGGCGGCCTCGCTTGGGAGGCTGTCAATAAGAACCTCATCTTCAATGTAACTCTCTCTCTCTCTCTACCTTCTTCTTCTTCTGGTTTCTTTGTTTGTTTGCTTTTAACCAAAGATTCAGGATTTACTTTCTCAGAATAAACGAATATTTCGTTCTTATCTTCAAAATTTTGAATCTGTCCTCACTCAATATTTCTAGGGTTCTTTTTCTTTTTCTTTTCTTTTATTTTTTTAATTTATCGATTTTGATGAAGGACCGCTAAATTGCTAATGCCATCTGAGTATCTGACCAATGGAAGTTTTTCACAGATTATTATATTTTTTCTTGTTAGAACTTTTGTTTCGATTTTTAAATTTTCTATTATTTTGATATTTGGGATTTTCTGATTTTCTATTTTGGTTTTTCAAAATTTACGTCAAAATATATTTTCACACTTGTGGTTAACATTTTTACATGTTCTACTCTTGATATGAATTTTGGATGCTGTCACATCAACTTTTCAATTATATGATATTATTCAGTTTTGTCGATTAAGTCAATTATATTGGATCTACTAAAAAAAATAAATAAATAAACGTGGCTAGAAACTTTTGTTTGTTCCTATAATAATCTCGGATAAGGAACTGGGCACACTATTTGAAAATTTGAGGACTATGATCTTACTTTAAATTACATGTTTTTTATTAGTTTTTTTTAAACGTTTTATAGTAGTAATAATTTAATTTTATTTTTGGATTAAGTTATTAATTTTTTTTCCTATAATTTAAGTTTAAGTTTAAGTTTAATTTGGTTTATTGGAGTGGAGCTTGATTTCAACCTGTTTTCTTTGATTTTATAATTTTTAATTTAGTTATCATAGTTCAGTTAATTCTCACAAATATTTTTGGAGATACAAGTTATTACTATGTTTACTTCATATGTATTTAAGTGGGTGTGTTAGTTTATTGTAATAAAATTTAAGGGAAAATGAGTGGACTTTTGGGTAAGTAAAAAATGGTTCCATTTGTATAATTTAACAAAATCACCATGGTTGAAAAAGAAAATCACACGTCCCAAATTGAAACCAGTACAGTTTAAAAACATCAAATTGAAACTCTTTAAATCATAGATATTAGGTTTAACTATTTTAATTAATTATTTTATTTTATTTTCCCTAAAAACGAAATTAGCCAAATAAAATAAAATAAAAGTGGTTGGGTTGGGAGGCCTTTGAATTTTGGCTGTTTTAATCGAGAGAGAGACCCACCTTTTAGGTCCTAGCTCTCAATTCCCACCTACCTTCATTTTTTTTTTTTTTTAAATATTCCCACCTACCTCCATTAAGGCTTTGGCATTGAGTTTTTTAATAAATTATAAATTATAAATTATTTTATTTGTATAATTTTATAATTCATTTTTTAAAGAACTGGATAAAGATATAGTCTCTATATTTTAGAGTTTCTATTTTGATTTTTTCTTTTTATAAATTTTTTTTCATTCAATTTTATATCTTCTATAATTGAGAAATTTATTTTTTGGTAGGGGGAAAATACGGACACACATCATGTTTTTAAATATTATTGATTTGGAAATTAATGGGTTGTTAGGAGTTGAATAAAAACTTAAGGGCAATAGAATATTAAAACTTTTTCCCCTTAATTTGTTTGTTAATTGGAACAGGTATGAATAGTCAATTAAGAACTAAGTTTGCAAAGGTTTAGAGCTAAAACTAGATTTAAGTTTAGGTATTAACATCTGACCTTAAACTAAATTTAAGGTAGTTGATGCCATCTCTTTGACTTGCAGATCAAAGAATCACAAGTAGCAACTTATAAATTATTTTGATGGATCATAGAGTAATTGCATGACCTGCTGCGAGTTTTATAATAGGAAAATTGATTATTTCTTTTGGTCCGGTACATAATTCTAATTTTTATATTTATATTTTAAAAGTTTTAAATTATTATTAAATGTATAATAAATATTTTAAAGAAGATTCTACCCAATAATAATGGAAACATGATATTAAAAAAGTATTAGCTTTTATGAGCCAATTATTTTAATATAAAAATTTTATCACATTCTACAAGAATTAAAAATAAAAAAATCCTGTCTCAAAAAGATAAAATAAAATAAAATAAAATAATCCAATACAGTTGGATGAAAGATGAATAAAATAGTGCATCATCATATAGATATCTTTATTTCAAAAAAAAAAAAAAAAAAAAAGATAGTTTATATCTCAAATAAAAAAATTCCCACCCTACCTATAAAAGATAATGTTTTAAAAATATTAATGATCTATTTGCATTACTTCTAGCTTAAACCCTTTTGAAAAATAATATTTTCAATAAAAATATTAGTATATCTTATATAATAACAAATTTATTTGTTTGCACTCTATTTTAAAAAGTGATTTATTCATTATAAATTTTTTTTTATTTTTTATTATATATTTGGAAGGTTTTCAATTAATTACTTTAAAGTATTTTTAATAATTCATTGATGAGGTGTTTGTTCAAAAAAGGTTTTTAATTAATTCAAATTTTTTAAACACCGTTTCTTCATTAAGATTTTTCTAAAAACTACTTCTTACCACATAAATTTCTTCAATCTTCTATTAAACCCACTCTGAATTATAATTTATTTATTTATTTATTTATTATTATTATTATTATTATTATTATTTTATAGATACTTAAAGAAAAACTGATTTTGTTAAAAAAAATATTTTTTTTCAGAAGTTATTCAAAACACATCATAAATCGTGATTTAATTTAAAGTTAGTTATTTGTGATATATATATTTGAGATATATTTGGGTTATAAATAACGCAGATAATGGTTGGAGTATAGATATATAATTTTGTGGATTGATTCTGGCATGATACCATGTGCTGATGGGTGCAAATCTCGAGTAAATTTTGAGCCAATCTGGAGGGACTGAACATGTTACATATGCTAAATAAATAAATAAATAAATATATACATATATATCCGCTTCCCTTTTTGTTTTTCCAGAAGAAAAACAAGACCGGATTTTATTTTTTCTAAATAAAATCTTATTCAAGTCAACAAATGAATGGTGAAGTTGAGGAACAATCAAAGTAGTTGGGAATGCCAACATTTCACATTTTTTTTCTTTTAAATTTGATGAGATTTGAGGGTGGGGGATTTGAAACTCCGTTTCTTGATCGAGGATACATGTCATTTTCTATAAGTCCATTTTGTCCATATCCACTTTGTAAAAATATATCTATTTATGAATATTTATTTAAATTATGCCTCAATTTTGATCTTGTTCGAAATTTTAAAACCAGATGTGGGAATAAGGAATTTAACTATTTACTTTAGGAAGGTAACTTAGTGCTTTAATTGAATGATTCTATACTTAGCTTGACATTCAATTTCAGTTTCAACTTTTGAATTTTTCATTTTTATAAACTTTTGAAGTTCCATATATGTCTTTTCATTTCTTGAATTGTTCGAAATCATCCTTTTATTCAACATTGTAAAAGAAATTATTAGTTTTAAATTGAACCAAGTTCTAATGTATATATATATTTATGTGAATAGATTCATCCTGTTCTTATGCTACTGGGATTGATCATTATAGGAGGCGAAGGTAATCAACTAAATTGAATTATATTGATTAGTTAATATCCAATTGTAAAGGGGGCAATAATTTGATTAGAAAAATGTGTTGAAAAATGTGCAGCAATAATTAGTTATAAATCACTGCCGCTGAGAAAGGAAGTGAAGAAAGTGATACATATGGTGCTACACGGCGTGGCATTGATACTGGGTATTGTGGGGATCAGCGCTGCATTCAAGAACCATAATGAAAGTGGGATCGCTAATCTTTACAGCTTGCATTCTTGGATTGGAATTGGCGTAATTGTCCTGTATGGCATCCAGGTATTTCCAAATCTGCCACCCATTTATTCTTCCTATACTTTTTCCAATTTAACTTTTATAATATAATAATTGTGTTAAAAGAAAGAAATTGCAATGCAATGCAATGGAATTTTGAATTTGCAGTGGGTGTACGGGTTGGTGACATTCTTGTACCCAGGAGGATCCTTGGATCTCCGAAGAGCGTCACTTCCATGGCACGTGGTATTGGGCATGATGATTTACATATTAGCCGTGGGCAACTCGGCGCTTGGGTTTCTAGAGAAGCTGACGTTTCTGGAGAGCTCTGGGGTCGCCAAATATGGCCCTGAGGCCTTCCTCGTCAACTTCACCGCCATCACCACTCTCATTTTTGCACTCTTTGTCATTCTCTCTGTTATTTCACAGCCCAAACCTCAAGACGATGACCACGCCGCCTATTCCGCTATATGATGCATGCTCTCCACTTTTTTCTTTTTCTTTTTCTATACGCTCAGGAAAGAAAGACATGTAAACAATTTCCAATTTCTTTTTTTCTATCCAAAACTTAATACTCGTGCTCTTATTCCTCCTTTATTAAACTTTTTATGTTTACTACACCTACACTTGTATTATCTGTTTTATTTTTTAATCACTCGTAAACAAATTGTCAACTAATGTAGTTTTCTTTGTAATTATTTATAATGGTTAATGGTTTTATGTTTGTTCTCTCTCTTATATTACATGTAACAGGGCGATGCTCTTTTCTTCTTTGTTAAGGACAATTCATCTTAATCCTGC

mRNA sequence

ATGGCGCTATCGGTGAAGGCTTTTCCGTTCACTGTGGTGAGTCACGTGCTCGCAATCGTGGCGTTGATTTTGGTGCTCATTTGGACCATTCACTTCAGAGGCGGCCTCGCTTGGGAGGCTGTCAATAAGAACCTCATCTTCAATGTAACTCTCTCTCTCTCTCTACCTTCTTCTTCTTCTGGTTTCTTTGTTTGTATGAATAGTCAATTAAGAACTAAGTTTGCAAAGATTCATCCTGTTCTTATGCTACTGGGATTGATCATTATAGGAGGCGAAGCAATAATTAGTTATAAATCACTGCCGCTGAGAAAGGAAGTGAAGAAAGTGATACATATGGTGCTACACGGCGTGGCATTGATACTGGGTATTGTGGGGATCAGCGCTGCATTCAAGAACCATAATGAAAGTGGGATCGCTAATCTTTACAGCTTGCATTCTTGGATTGGAATTGGCGTAATTGTCCTGTATGGCATCCAGTGGGTGTACGGGTTGGTGACATTCTTGTACCCAGGAGGATCCTTGGATCTCCGAAGAGCGTCACTTCCATGGCACGTGGTATTGGGCATGATGATTTACATATTAGCCGTGGGCAACTCGGCGCTTGGGTTTCTAGAGAAGCTGACGTTTCTGGAGAGCTCTGGGGTCGCCAAATATGGCCCTGAGGCCTTCCTCGTCAACTTCACCGCCATCACCACTCTCATTTTTGCACTCTTTGTCATTCTCTCTGTTATTTCACAGCCCAAACCTCAAGACGATGACCACGCCGCCTATTCCGCTATATGA

Coding sequence (CDS)

ATGGCGCTATCGGTGAAGGCTTTTCCGTTCACTGTGGTGAGTCACGTGCTCGCAATCGTGGCGTTGATTTTGGTGCTCATTTGGACCATTCACTTCAGAGGCGGCCTCGCTTGGGAGGCTGTCAATAAGAACCTCATCTTCAATGTAACTCTCTCTCTCTCTCTACCTTCTTCTTCTTCTGGTTTCTTTGTTTGTATGAATAGTCAATTAAGAACTAAGTTTGCAAAGATTCATCCTGTTCTTATGCTACTGGGATTGATCATTATAGGAGGCGAAGCAATAATTAGTTATAAATCACTGCCGCTGAGAAAGGAAGTGAAGAAAGTGATACATATGGTGCTACACGGCGTGGCATTGATACTGGGTATTGTGGGGATCAGCGCTGCATTCAAGAACCATAATGAAAGTGGGATCGCTAATCTTTACAGCTTGCATTCTTGGATTGGAATTGGCGTAATTGTCCTGTATGGCATCCAGTGGGTGTACGGGTTGGTGACATTCTTGTACCCAGGAGGATCCTTGGATCTCCGAAGAGCGTCACTTCCATGGCACGTGGTATTGGGCATGATGATTTACATATTAGCCGTGGGCAACTCGGCGCTTGGGTTTCTAGAGAAGCTGACGTTTCTGGAGAGCTCTGGGGTCGCCAAATATGGCCCTGAGGCCTTCCTCGTCAACTTCACCGCCATCACCACTCTCATTTTTGCACTCTTTGTCATTCTCTCTGTTATTTCACAGCCCAAACCTCAAGACGATGACCACGCCGCCTATTCCGCTATATGA

Protein sequence

MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSSGFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALILGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRASLPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVILSVISQPKPQDDDHAAYSAI

Homology

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BLAST of Spg038953 vs. NCBI nr
Match: XP_038888144.1 (transmembrane ascorbate ferrireductase 1 [Benincasa hispida])

HSP 1 Score: 392.1 bits (1006), Expect = 3.7e-105
Identity = 208/260 (80.00%), Postives = 219/260 (84.23%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFPFTVVSHVLAIVALI+VLIWTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPFTVVSHVLAIVALIMVLIWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHLVLHGVALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LYG+QWVYG VTF YPGGS DLRR S
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYGLQWVYGFVTFFYPGGSADLRRVS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHV+ G+ +YILAVGNSALGFLEKLTFLESSG+AKYG EAFLVNFTAITT+IFA+FVI
Sbjct: 181 LPWHVIFGLTVYILAVGNSALGFLEKLTFLESSGLAKYGTEAFLVNFTAITTVIFAIFVI 232

Query: 241 LSVISQPKPQDDDHAAYSAI 261
           LSVISQP  Q+DDH AYSAI
Sbjct: 241 LSVISQPCAQEDDHGAYSAI 232

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BLAST of Spg038953 vs. NCBI nr
Match: BAE46406.1 (cytochrome b561 [Citrullus lanatus])

HSP 1 Score: 389.0 bits (998), Expect = 3.1e-104
Identity = 208/260 (80.00%), Postives = 219/260 (84.23%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFPFTVVSHVLAIVALILVL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPFTVVSHVLAIVALILVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHLVLHGVALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LYG+QWVYG VTF YPGGS DLRRAS
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYGLQWVYGFVTFFYPGGSADLRRAS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHV+ G+ IYILAVGNSALGFLEKLTFLESSG+AKYG EAFLVNFTAITT++FALFVI
Sbjct: 181 LPWHVLFGITIYILAVGNSALGFLEKLTFLESSGLAKYGTEAFLVNFTAITTVVFALFVI 232

Query: 241 LSVISQPKPQDDDHAAYSAI 261
           LSVISQP  Q+D+H  YSAI
Sbjct: 241 LSVISQPCVQEDNHGDYSAI 232

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BLAST of Spg038953 vs. NCBI nr
Match: XP_008465873.1 (PREDICTED: transmembrane ascorbate ferrireductase 1 [Cucumis melo] >KAA0038497.1 transmembrane ascorbate ferrireductase 1 [Cucumis melo var. makuwa] >TYK31091.1 transmembrane ascorbate ferrireductase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 374.8 bits (961), Expect = 6.1e-100
Identity = 199/261 (76.25%), Postives = 213/261 (81.61%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFP TVVSHV+ IVA+ILVL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAVILVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHP LMLLGLI+IGGEAIISYKSLPLRKEVKKVIH+VLHGVAL+
Sbjct: 61  ----------------IHPFLMLLGLIVIGGEAIISYKSLPLRKEVKKVIHLVLHGVALV 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LY +QWVYG VTF YPGGS DLRR S
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYALQWVYGFVTFFYPGGSADLRRVS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV G+ +YILAVGNSALGFLEKLTFLESSG+ KYG EAFLVNFTAITT+IF +FVI
Sbjct: 181 LPWHVVFGLTVYILAVGNSALGFLEKLTFLESSGIPKYGTEAFLVNFTAITTIIFGVFVI 233

Query: 241 LSVISQPKPQ-DDDHAAYSAI 261
            SVISQP  Q +DDH AYSAI
Sbjct: 241 FSVISQPCAQPEDDHGAYSAI 233

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BLAST of Spg038953 vs. NCBI nr
Match: XP_004148357.1 (transmembrane ascorbate ferrireductase 1 [Cucumis sativus] >KGN60469.1 hypothetical protein Csa_002510 [Cucumis sativus])

HSP 1 Score: 374.4 bits (960), Expect = 8.0e-100
Identity = 199/261 (76.25%), Postives = 214/261 (81.99%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFP TVVSHV+ IVA+I+VL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAMIMVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAI+SYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIMSYKSLPLRKEVKKVIHLVLHGVALG 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LYG+QWVYG  TF YPGGS DLRR S
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYGLQWVYGFATFFYPGGSGDLRRVS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV G+ +Y+LAVGNSALGFLEKLTFLES G+ KYG EAFLVNFTAITT+IFA+FVI
Sbjct: 181 LPWHVVFGLAVYVLAVGNSALGFLEKLTFLESGGIPKYGTEAFLVNFTAITTIIFAVFVI 233

Query: 241 LSVISQP-KPQDDDHAAYSAI 261
           LSVISQP   Q+DDH AYSAI
Sbjct: 241 LSVISQPCAQQEDDHGAYSAI 233

[Close]

BLAST of Spg038953 vs. NCBI nr
Match: XP_022953106.1 (transmembrane ascorbate ferrireductase 1 [Cucurbita moschata] >KAG6571805.1 Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.5 bits (937), Expect = 3.7e-97
Identity = 196/260 (75.38%), Postives = 208/260 (80.00%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           M L VKAFPFTVVSHVLAIVALILVL+W +HFRGGLAWEAVNKNLIFN            
Sbjct: 1   MGLPVKAFPFTVVSHVLAIVALILVLVWAVHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHLVLHGVALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGI NLYSLHSWIGIGVI LYG+QWVYGLVTF YPGGS DLRRAS
Sbjct: 121 LGIVGIWAAFKNHNESGIPNLYSLHSWIGIGVIALYGVQWVYGLVTFFYPGGSADLRRAS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV+G+ +YILAVGNSALGFLEKLTFLES GV KYG EAFLVNFTAI T++F   V+
Sbjct: 181 LPWHVVVGISVYILAVGNSALGFLEKLTFLESGGVDKYGSEAFLVNFTAIITIVFGASVL 232

Query: 241 LSVISQPKPQDDDHAAYSAI 261
           LSVISQ   +  D  AY+AI
Sbjct: 241 LSVISQHSVEQHDADAYTAI 232

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BLAST of Spg038953 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.3e-78
Identity = 153/267 (57.30%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MA+ + A   T V+H LA++A I+VL+W+I +RGGLAWEA NKNLIFN            
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           +HPVLML+G II+GGEAIISYKSLPL K VKK+IH++LH +AL 
Sbjct: 61  ----------------LHPVLMLIGFIILGGEAIISYKSLPLEKPVKKLIHLILHAIALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGI GI AAFKNHNES I NLYSLHSWIGIGVI LYG QWVY  + F +PGGS +L+   
Sbjct: 121 LGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNLKSGL 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWH +LG+ +YILAVGN+ALGFLEKLTFLE+ G+ KYG EAFL+NFTAI T++F  FV+
Sbjct: 181 LPWHAMLGLFVYILAVGNAALGFLEKLTFLENGGLDKYGSEAFLINFTAIITILFGAFVV 239

Query: 241 LSVISQ-----PKPQDDDHA--AYSAI 261
           L+  ++     P   +DD    +YSAI
Sbjct: 241 LTASAESPSPSPSVSNDDSVDFSYSAI 239

[Close]

BLAST of Spg038953 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.1e-70
Identity = 138/246 (56.10%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           + L V+A PFT  +H LA+ A  +VL+W I+FRGGLA EA NKNLIFNV           
Sbjct: 3   LGLGVRAAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNV----------- 62

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPL-RKEVKKVIHMVLHGVAL 120
                            HPVLML+G IIIGGEAI+ Y+ LP    E  K+IH+VLHG+AL
Sbjct: 63  -----------------HPVLMLIGYIIIGGEAIMVYRVLPTSNHETNKLIHLVLHGIAL 122

Query: 121 ILGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRA 180
           +LG VGI  AFKNHNESGIANLYSLHSWIGIG I LYGIQW+ G VTF +PG + ++++ 
Sbjct: 123 VLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIVGFVTFFFPGAAPNVKKG 182

Query: 181 SLPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFV 240
            LPWH++ G+ +YILA+ N+ LGFLEKLTFLESSG+ KYG EAFLVNFTA+  ++F   V
Sbjct: 183 VLPWHILFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFGASV 220

Query: 241 ILSVIS 246
           +++ I+
Sbjct: 243 VVAAIA 220

[Close]

BLAST of Spg038953 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 3.4e-69
Identity = 135/246 (54.88%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           + L V+A PFT  +H LA+ A  +VL+W+I FRGGLA E+ NKNLIFNV           
Sbjct: 3   LGLGVRAAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNV----------- 62

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPL-RKEVKKVIHMVLHGVAL 120
                            HPVLML+G +IIGGEAI+ Y+ LP    +  K+IH++LHG+AL
Sbjct: 63  -----------------HPVLMLIGYVIIGGEAIMVYRVLPTSNHDTTKLIHLILHGIAL 122

Query: 121 ILGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRA 180
           +LG VGI  AFKNHNESGIANLYSLHSWIGIG I LYGIQW+ G VTF +PG + ++++ 
Sbjct: 123 VLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIIGFVTFFFPGAAPNVKKG 182

Query: 181 SLPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFV 240
            LPWHV+ G+ +YILA+ N+ LGFLEKLTFLESSG+ KYG EAFLVNFTA+  ++F   V
Sbjct: 183 VLPWHVLFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFGASV 220

Query: 241 ILSVIS 246
           +++ I+
Sbjct: 243 VVAAIA 220

[Close]

BLAST of Spg038953 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 4.9e-68
Identity = 133/252 (52.78%), Postives = 172/252 (68.25%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           M L V+A PFT V+H LA+ A  +VL+W IHFRGGLA+EA NKNLIFNV           
Sbjct: 1   MGLGVRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNV----------- 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRK-EVKKVIHMVLHGVAL 120
                            HPVLML+G II+G EAI+ YK LP  K +  K+IH++LH +AL
Sbjct: 61  -----------------HPVLMLIGYIILGSEAIMVYKVLPTWKHDTTKLIHLILHAIAL 120

Query: 121 ILGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRA 180
           + G VGI  AFK HNESGIANLYSLHSW+GIG I LYGIQW++G V F +P  S  +R+ 
Sbjct: 121 VFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTICLYGIQWIFGFVAFFFPRASPSVRKG 180

Query: 181 SLPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFV 240
            LPWH++ G+ +YILA+  + LGFLEKLTFL+SSG+ KYG EAFLVNFTA+  ++F   V
Sbjct: 181 VLPWHILFGLFVYILALATAELGFLEKLTFLQSSGLDKYGAEAFLVNFTALIVVLFGASV 224

Query: 241 ILSVISQPKPQD 252
           +++ +S  + ++
Sbjct: 241 VVAAVSPARVEE 224

[Close]

BLAST of Spg038953 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 6.0e-66
Identity = 129/244 (52.87%), Postives = 166/244 (68.03%), Query Frame = 0


Query: 3   LSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSSGF 62
           L VKA PFT V+H LA+ A ++VL+W I FRGGLA+EA NKNLIFNV             
Sbjct: 5   LGVKAAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNV------------- 64

Query: 63  FVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLP-LRKEVKKVIHMVLHGVALIL 122
                          HPVLML+G II+G EAI+ YK  P L  +  K+IH++LH +A++L
Sbjct: 65  ---------------HPVLMLIGYIILGSEAIMIYKIFPKLNHDTTKLIHLILHAIAIVL 124

Query: 123 GIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRASL 182
           G VGI  AFK HNESGIANLYSLHSW+GIG I LYGIQW++G V F YPG +  +RR +L
Sbjct: 125 GAVGIYCAFKFHNESGIANLYSLHSWLGIGTISLYGIQWIFGFVAFFYPGAAPHVRRGAL 184

Query: 183 PWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVIL 242
           PWHV+ G+ +Y+L +  + LG LEKLTFL+SSG+ KYG EAFLVNFT +   +F   V++
Sbjct: 185 PWHVLFGLFVYVLTLATAELGLLEKLTFLQSSGLDKYGAEAFLVNFTGLVVALFGAAVVV 220

Query: 243 SVIS 246
           + ++
Sbjct: 245 AAVA 220

[Close]

BLAST of Spg038953 vs. ExPASy TrEMBL
Match: Q3LGX5 (Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561A PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.5e-104
Identity = 208/260 (80.00%), Postives = 219/260 (84.23%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFPFTVVSHVLAIVALILVL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPFTVVSHVLAIVALILVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHLVLHGVALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LYG+QWVYG VTF YPGGS DLRRAS
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYGLQWVYGFVTFFYPGGSADLRRAS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHV+ G+ IYILAVGNSALGFLEKLTFLESSG+AKYG EAFLVNFTAITT++FALFVI
Sbjct: 181 LPWHVLFGITIYILAVGNSALGFLEKLTFLESSGLAKYGTEAFLVNFTAITTVVFALFVI 232

Query: 241 LSVISQPKPQDDDHAAYSAI 261
           LSVISQP  Q+D+H  YSAI
Sbjct: 241 LSVISQPCVQEDNHGDYSAI 232

[Close]

BLAST of Spg038953 vs. ExPASy TrEMBL
Match: A0A5A7TAA1 (Transmembrane ascorbate ferrireductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003680 PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 3.0e-100
Identity = 199/261 (76.25%), Postives = 213/261 (81.61%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFP TVVSHV+ IVA+ILVL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAVILVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHP LMLLGLI+IGGEAIISYKSLPLRKEVKKVIH+VLHGVAL+
Sbjct: 61  ----------------IHPFLMLLGLIVIGGEAIISYKSLPLRKEVKKVIHLVLHGVALV 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LY +QWVYG VTF YPGGS DLRR S
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYALQWVYGFVTFFYPGGSADLRRVS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV G+ +YILAVGNSALGFLEKLTFLESSG+ KYG EAFLVNFTAITT+IF +FVI
Sbjct: 181 LPWHVVFGLTVYILAVGNSALGFLEKLTFLESSGIPKYGTEAFLVNFTAITTIIFGVFVI 233

Query: 241 LSVISQPKPQ-DDDHAAYSAI 261
            SVISQP  Q +DDH AYSAI
Sbjct: 241 FSVISQPCAQPEDDHGAYSAI 233

[Close]

BLAST of Spg038953 vs. ExPASy TrEMBL
Match: A0A1S3CPV4 (transmembrane ascorbate ferrireductase 1 OS=Cucumis melo OX=3656 GN=LOC103503461 PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 3.0e-100
Identity = 199/261 (76.25%), Postives = 213/261 (81.61%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFP TVVSHV+ IVA+ILVL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAVILVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHP LMLLGLI+IGGEAIISYKSLPLRKEVKKVIH+VLHGVAL+
Sbjct: 61  ----------------IHPFLMLLGLIVIGGEAIISYKSLPLRKEVKKVIHLVLHGVALV 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LY +QWVYG VTF YPGGS DLRR S
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYALQWVYGFVTFFYPGGSADLRRVS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV G+ +YILAVGNSALGFLEKLTFLESSG+ KYG EAFLVNFTAITT+IF +FVI
Sbjct: 181 LPWHVVFGLTVYILAVGNSALGFLEKLTFLESSGIPKYGTEAFLVNFTAITTIIFGVFVI 233

Query: 241 LSVISQPKPQ-DDDHAAYSAI 261
            SVISQP  Q +DDH AYSAI
Sbjct: 241 FSVISQPCAQPEDDHGAYSAI 233

[Close]

BLAST of Spg038953 vs. ExPASy TrEMBL
Match: A0A0A0LHJ1 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914000 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.9e-100
Identity = 199/261 (76.25%), Postives = 214/261 (81.99%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MAL+VKAFP TVVSHV+ IVA+I+VL+WTIHFRGGLAWEAVNKNLIFN            
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAMIMVLVWTIHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAI+SYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIMSYKSLPLRKEVKKVIHLVLHGVALG 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGIANLYSLHSWIGIGVI LYG+QWVYG  TF YPGGS DLRR S
Sbjct: 121 LGIVGIIAAFKNHNESGIANLYSLHSWIGIGVISLYGLQWVYGFATFFYPGGSGDLRRVS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV G+ +Y+LAVGNSALGFLEKLTFLES G+ KYG EAFLVNFTAITT+IFA+FVI
Sbjct: 181 LPWHVVFGLAVYVLAVGNSALGFLEKLTFLESGGIPKYGTEAFLVNFTAITTIIFAVFVI 233

Query: 241 LSVISQP-KPQDDDHAAYSAI 261
           LSVISQP   Q+DDH AYSAI
Sbjct: 241 LSVISQPCAQQEDDHGAYSAI 233

[Close]

BLAST of Spg038953 vs. ExPASy TrEMBL
Match: A0A6J1GM28 (transmembrane ascorbate ferrireductase 1 OS=Cucurbita moschata OX=3662 GN=LOC111455609 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.8e-97
Identity = 196/260 (75.38%), Postives = 208/260 (80.00%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           M L VKAFPFTVVSHVLAIVALILVL+W +HFRGGLAWEAVNKNLIFN            
Sbjct: 1   MGLPVKAFPFTVVSHVLAIVALILVLVWAVHFRGGLAWEAVNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIH+VLHGVAL 
Sbjct: 61  ----------------IHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHLVLHGVALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGIVGI AAFKNHNESGI NLYSLHSWIGIGVI LYG+QWVYGLVTF YPGGS DLRRAS
Sbjct: 121 LGIVGIWAAFKNHNESGIPNLYSLHSWIGIGVIALYGVQWVYGLVTFFYPGGSADLRRAS 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWHVV+G+ +YILAVGNSALGFLEKLTFLES GV KYG EAFLVNFTAI T++F   V+
Sbjct: 181 LPWHVVVGISVYILAVGNSALGFLEKLTFLESGGVDKYGSEAFLVNFTAIITIVFGASVL 232

Query: 241 LSVISQPKPQDDDHAAYSAI 261
           LSVISQ   +  D  AY+AI
Sbjct: 241 LSVISQHSVEQHDADAYTAI 232

[Close]

BLAST of Spg038953 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 293.5 bits (750), Expect = 1.7e-79
Identity = 153/267 (57.30%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MALSVKAFPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSS 60
           MA+ + A   T V+H LA++A I+VL+W+I +RGGLAWEA NKNLIFN            
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFN------------ 60

Query: 61  GFFVCMNSQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALI 120
                           +HPVLML+G II+GGEAIISYKSLPL K VKK+IH++LH +AL 
Sbjct: 61  ----------------LHPVLMLIGFIILGGEAIISYKSLPLEKPVKKLIHLILHAIALA 120

Query: 121 LGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRAS 180
           LGI GI AAFKNHNES I NLYSLHSWIGIGVI LYG QWVY  + F +PGGS +L+   
Sbjct: 121 LGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNLKSGL 180

Query: 181 LPWHVVLGMMIYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVI 240
           LPWH +LG+ +YILAVGN+ALGFLEKLTFLE+ G+ KYG EAFL+NFTAI T++F  FV+
Sbjct: 181 LPWHAMLGLFVYILAVGNAALGFLEKLTFLENGGLDKYGSEAFLINFTAIITILFGAFVV 239

Query: 241 LSVISQ-----PKPQDDDHA--AYSAI 261
           L+  ++     P   +DD    +YSAI
Sbjct: 241 LTASAESPSPSPSVSNDDSVDFSYSAI 239

[Close]

BLAST of Spg038953 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 185.3 bits (469), Expect = 6.4e-47
Identity = 101/245 (41.22%), Postives = 143/245 (58.37%), Query Frame = 0


Query: 8   FPFTVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSSGFFVCMN 67
           FP  +V  VL  +   LVL WT+H+RGGLA  + NK+ IFNV                  
Sbjct: 9   FPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNV------------------ 68

Query: 68  SQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALILGIVGIS 127
                     HPV+M++GLI+  GEA+++YKS+   K +KK++H+ L   A IL ++G+ 
Sbjct: 69  ----------HPVMMVIGLILFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVW 128

Query: 128 AAFKNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRASLPWHVVL 187
           AA K H + GI N YSLHSW+G+  + L+  QW  G VT+ YPGGS + R + +PWHV L
Sbjct: 129 AALKFHIDKGIENFYSLHSWLGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFL 188

Query: 188 GMMIYILAVGNSALGFLEKLTFLE-SSGVAKYGPEAFLVNFTAITTLIFALFVILSVISQ 247
           G+ IY LA+  +  G LEK+TFL+ +  + +Y  EA LVN   +  LI   FVIL V++ 
Sbjct: 189 GISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTP 225

Query: 248 PKPQD 252
              +D
Sbjct: 249 VSGKD 225

[Close]

BLAST of Spg038953 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-31
Identity = 77/233 (33.05%), Postives = 127/233 (54.51%), Query Frame = 0


Query: 11  TVVSHVLAIVALILVLIWTIHFRGGLAWEAVNKNLIFNVTLSLSLPSSSSGFFVCMNSQL 70
           T+V+H   I+A++L+LIW +H+R G+ + + N   + NV                     
Sbjct: 18  TLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNV--------------------- 77

Query: 71  RTKFAKIHPVLMLLGLIIIGGEAIISYKSLPLRKEVKKVIHMVLHGVALILGIVGISAAF 130
                  HP LM  G + + G+A+++YK+     +V+K++H  LH + L+LGIVGI AAF
Sbjct: 78  -------HPFLMYCGFLFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAF 137

Query: 131 KNHNESGIANLYSLHSWIGIGVIVLYGIQWVYGLVTFLYPGGSLDLRRASLPWHVVLGMM 190
           + H++  + ++ SLHSWIG+   +L G+QW++G  TFL P  S   R   +PWHV+ G  
Sbjct: 138 RFHDKVNLKDMVSLHSWIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRA 197

Query: 191 IYILAVGNSALGFLEKLTFLESSGVAKYGPEAFLVNFTAITTLIFALFVILSV 244
           +  + +  +  G +++ T L  S  A    E+ L+NF  +  L+F + V  SV
Sbjct: 198 LLYMGIVAALTGLMQRATMLGQSTNA----ESRLINFLGLAILLFGVSVDFSV 218

[Close]

BLAST of Spg038953 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 125.9 bits (315), Expect = 4.6e-29
Identity = 81/222 (36.49%), Postives = 122/222 (54.95%), Query Frame = 0


Query: 51  LSLSLPSSSSGFFVCMN--------SQLRTKFAKIHPVLMLLGLIIIGGEAIISYKSLPL 110
           LSL L +  SG  V ++          L   ++ +HP+LM++G I++ GEAI+ ++ LP 
Sbjct: 9   LSLVLFARLSGLVVAVSVLYWALFLPNLGLSYSTLHPLLMVIGFILVSGEAILIHRWLPG 68

Query: 111 RKEVKKVIHMVLHGVALILGIVGISAAFKNHNESGI-ANLYSLHSWIGIGVIVLYGIQWV 170
            ++ KK +H+ L G+AL   + GI   F  H + G+ AN YSLHSW+G+  + L+  QWV
Sbjct: 69  SRKTKKAVHLWLQGMALASAVFGIWTKF--HYQRGVFANFYSLHSWMGLLSVSLFAAQWV 128

Query: 171 YGLVTFLYPGGSLDLRRASLPWHVVLGMMIYILAVGNSALGFLEKLTFLESS-GVAKYGP 230
            G ++F + G     R   LPWHV LG+  Y LA+  +  G LEKLTFL++   V + G 
Sbjct: 129 TGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGS 188

Query: 231 EAFLVNFTAITTLIFALFVILSVISQPKPQD---DDHAAYSA 260
           E+  VN   +   +    VI + I  PK Q    D+   YS+
Sbjct: 189 ESMTVNGLGLGLALLGCIVITAAI-LPKYQSHSRDEKLVYSS 227

The following BLAST results are available for this feature:

BLAST of Spg038953 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888144.1	3.7e-105	80.00	transmembrane ascorbate ferrireductase 1 [Benincasa hispida]	[more]
BAE46406.1	3.1e-104	80.00	cytochrome b561 [Citrullus lanatus]	[more]
XP_008465873.1	6.1e-100	76.25	PREDICTED: transmembrane ascorbate ferrireductase 1 [Cucumis melo] >KAA0038497.1...	[more]
XP_004148357.1	8.0e-100	76.25	transmembrane ascorbate ferrireductase 1 [Cucumis sativus] >KGN60469.1 hypotheti...	[more]
XP_022953106.1	3.7e-97	75.38	transmembrane ascorbate ferrireductase 1 [Cucurbita moschata] >KAG6571805.1 Tran...	[more]

BLAST of Spg038953 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L856	2.3e-78	57.30	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
C4IYS8	3.1e-70	56.10	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
Q6I681	3.4e-69	54.88	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]
A3A9H6	4.9e-68	52.78	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]
Q7XMK3	6.0e-66	52.87	Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa...	[more]

BLAST of Spg038953 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q3LGX5	1.5e-104	80.00	Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561A PE=2 SV=1	[more]
A0A5A7TAA1	3.0e-100	76.25	Transmembrane ascorbate ferrireductase 1 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3CPV4	3.0e-100	76.25	transmembrane ascorbate ferrireductase 1 OS=Cucumis melo OX=3656 GN=LOC103503461...	[more]
A0A0A0LHJ1	3.9e-100	76.25	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G91...	[more]
A0A6J1GM28	1.8e-97	75.38	transmembrane ascorbate ferrireductase 1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Spg038953 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25570.1	1.7e-79	57.30	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT5G38630.1	6.4e-47	41.22	cytochrome B561-1	[more]
AT1G14730.1	1.3e-31	33.05	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G26100.1	4.6e-29	36.49	Cytochrome b561/ferric reductase transmembrane protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 77..203 e-value: 3.8E-51 score: 185.9
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 77..209 e-value: 7.3E-44 score: 149.2
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 13..244 score: 41.521877
None	No IPR available	GENE3D	1.20.120.1770		coord: 2..255 e-value: 1.6E-75 score: 255.1
None	No IPR available	PANTHER	PTHR10106:SF22	TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 1	coord: 1..50
None	No IPR available	PANTHER	PTHR10106:SF22	TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 1	coord: 67..260
None	No IPR available	CDD	cd08766	Cyt_b561_ACYB-1_like	coord: 76..211 e-value: 2.14506E-64 score: 195.989
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 67..260 coord: 1..50

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg038953.1	Spg038953.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity