Spg038881 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038881
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Locationscaffold12: 7110928 .. 7114703 (-)
RNA-Seq ExpressionSpg038881
SyntenySpg038881
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAAGCCTCTGATGTCATTTGGGAGGGGAGAATGGACCAATATAGACAAAACCATTGCATTGGTTCTTTTCTTTTTGCATGTGCCCTGTATTTTTGCACCATTTCAATTCACTTGGGGTGCATTTTGGGTTGCCTTCGTGTTATATGTTATCACAGGTCTTTCCGGTATTACTATTTCATATCATAGAAATCTTTCACACAAGAGTTTCAAGCTTCCCAAATGGCTCGAATATTTGTTTGCTTATTGTGGAGTTCATGCATTTCAGGTTTGATATTTGAACCATATGAATATATGAATAAATCTATGAGATTCTGAAAGTTTTTATTTGTTGTTTATATTTTAGGGTGATCCGATCGATTGGGTGAGTACGCATAGGTGTCACCATCAATTTGTTGATACCGAAAAAGACCCGCACAGTCCTATTCATGGATTTTGGTTCAGTCATATTGCTTGGACTTTAGATTCGTACCGTTTGACTAAAAATGTTTGTCCAAAATACTTTAGTGATTTTAAAGAGATGGAAAGAAACATCTTCATGATGGCTATGAAGCATGGGAAACCAACTAATGTTGGAGATTTGGAGAAACAAACTTTTTATAGGTTTATTCAGAAAACATATTTTCTTCATCCAATTTCTCTTGCTGTGCTGCTCTATTCTATTGGAGGAATCCCTTTTGTTATATGGGGAATGGTAAGATATGATCACCATATGATGCTTAAAATAGATCATCTTATTTACATTATTATCATTTTAATAACAAGGCTTTAATAAATTAATTTCAATTTAACAAAATATGAAAAAGTGTATGTATTTTTCACAAATGAAAAGGTAACTGAGAAAAGAGCAATTAAATTATTTCTCATGGTATCTTGCCAGTTAATTTTGAACAATACTTGGCTGCATACCGGTATATGTAGGGATCCCTACATACCACATTAAAATTTTGACAACTAAAAAAGAGAAAGATAGAAAATGAGAAGAAAAAGAATTTATCTCTCATGGTTAATTATCAACATTTTAATAGTCCCTACATACCAATTCTCCCTGCATATCGGTATACAGCCAAGTTTTTTCCTTTAATTTTTATTTGTAGAATAGTTATGCATGTAAACTTTCAATTTACTCTAAAGCCTGTATAAGTTTCCAGGATTTTATAATTCTTCAATTATTACATAAAAATGTTAAAAATTTTTGGAATATTTTAGACTTTTAAATACAAATAGAATATTTTAAAAATTCATGTTTGGAGAAATAATACTAAATAGTAATTGTATTAATCTTACTGCAGTGTGTGAGGATGGTAATGTTCGTACATGTAATGTTTATGGTAAATTCAATATGCCATACATGGGGAAAACGTCAGTGGAATACGAGTGATTTATCCAGAAATAATTGGTAAGTATTGTTTTTACTCGAGTCCGTTTGGAATACATTTTGATTTAAATGTTTTTCAAGAATGCATTTTTTAAAAAAAAAAATTATATAAAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTGCATTTCCTCGCAAATTTTTTTTATACATAAGTTATCGTTTACTTAAAAAGTCTTTTTTAAAAGGTTGGTTTCAAGTGTTTCTCTCAAAATGGTTTATTTATCAATCTCATTTTCTAATAAGTCGTTTTTAGTGGTTGTCAAACACTAGAAATTTTTTCAAAATAATTTATTTTCAAAATTAAACACTTAAAAGTTAAACCAAACACACCCTCCATTATCAAATGAAAGGAAATGATTATTTACAATAATTGATTACTATTTTGAGCCTTTGTTGCAGATATTGCAATACTCTTTAGATAGAAATTTTAATGAAAATTGACCAGATTTATTTAGGCATCGTTTGGTAACCATTTGGTTTTTGGTTTTTGAAAATTAAGCTTATAAATCCTACTTCGTTTCTTTGTTTTGCTAATACTTTTTTTTTCCTTGTTTTTAAAAACCAAGCAAATTTTGAAAACTAAAAAAAGCAGTTTTCAAAACCTTGTTTTTGTTTTTAGAATTTGGCTAGGAACTCAAATGTGTCATTGGCAAATATGAAAACCATGGTAGGAAAATTGATAGAAAACAAGCCTAATTTTCAAAAACCAAATGGTTATCAAATGAGACCTTATTCATTTATTTTGAGTTCAACAGATTTGGGATAGGAGATTTGAACTTTTGACTTCTTGGTCAAGAATACAACTCAAATACCACTAAGCTATGTTGGTTATTTAATGTAGCACAGATCAGTGGTTTCTCAAATAAAAAAAATGTGGCAAAGAAATTACTAACATTTCATATATACATTTGATTATATGATTTTTAACCCAAAAAAAAAAAGTAAATAAGTACCAATTTTTTTTCTAGTAAACCAATTTAATTGCTGTTAGGATTATTAGTATAAAAATGAAATTATTTTTTTTTTTGAAAAAAAAAATGAAATTATTTAACTATTAGTTCATAGTTATACTTCATTAATGCTATTTTTATTTTGATGATTCAATACTATTAAGTAAATTTCATAATTACCCACTTAACTCAATTTATTAAGATATATACCTTCAATCATGAAGTTTTTTTTTTAGAAAAATATAATTCCATTTTTGAAACTAAAAAGATGATGAGAGACATGACCCACACTATTCAATAACTTGATAAATGGAATTCGATGATTATAGAAGGTGTATTTAGGGTACATTTGGCGTTGATTTTAGAAGCAGTTAAAGTGATTTTAAAAGAATCTTAATCGTTTTTAGCGTTTGGTAAAACAGATTATAAATTAATTTTTATAAAAGTACTTTTTCAACCACGTTCATTTGCAAAATCTAATAATCGTAGCTTTTCCGTTTAGCTTTCCTAAAAATCTAATCTGTAACTAACTATTTATCTTCTCTCTACGTTTTTTAGACTTGATTCATGTGAATCATTTTTTTAATTTAATATATTTTTATTTTATTATTTTATACTCATTTGAAATTATTTGTGTTTATTTTTATTTAAATTAAAATAATATTTTTTCCAATACAAGTGGCAAATTATATTTCTTAGCAAAATATAAAAACATAATAGGTTAAACATAAGATTATATTTTAAATCATACGAAATTTAAAACAATTATCTTATATGTTTCAAAAAAATAATTATCTTAAAATAATTTGGAAATTATCTTTTAAAATTTTGAAATTAAATATCAATATTAAATTTATTTATTGAAAAAATATAGTTAAGTTGCGATAATTTAGAATATTAATTTACCAAACACCTCAACTTACTTTTTTGTCTATAAAGCTGAAATTTACCAAACACTAATTTACTTCGTAATACAGCTGATTTTCTTAAAAGCACAGTTAACAGCAATTATTTTAGAAGCTACAACAACGCCAAACTAAGCCTTAGAAGTAATCAACTCAACTCTGGTTAAGACATTATACATCTTTCTCGAAGGTCAAATGTTCACATTTCCCTCTCAATATTATTGTTGAACTAAAAAAAAACTATTTTTTTAATTAGAGTATTACATTTTGCTTTTTTTTCTCTCTCTCCTTAAGGTGGGTAGGTTTGCTTTCATTTGGAGAAGGATGGCATAATAACCACCATGCCTTTGAGTATTCAGCTAGACATGGACTTGAATGGTGGCAACTTGACTTTAGTTGGTATGTCATTATGTTCCTTCAAGCCATTGGAGTGGCCACTGAGGTAAAATTACCTTCTCAAAGTCACAAGGAAAGATTGGCTATGGACAAACTAAAAGAAGACATTCCAAGATGA

mRNA sequence

ATGGTGAAGCCTCTGATGTCATTTGGGAGGGGAGAATGGACCAATATAGACAAAACCATTGCATTGGTTCTTTTCTTTTTGCATGTGCCCTGTATTTTTGCACCATTTCAATTCACTTGGGGTGCATTTTGGGTTGCCTTCGTGTTATATGTTATCACAGGTCTTTCCGGTATTACTATTTCATATCATAGAAATCTTTCACACAAGAGTTTCAAGCTTCCCAAATGGCTCGAATATTTGTTTGCTTATTGTGGAGTTCATGCATTTCAGGGTGATCCGATCGATTGGGTGAGTACGCATAGGTGTCACCATCAATTTGTTGATACCGAAAAAGACCCGCACAGTCCTATTCATGGATTTTGGTTCAGTCATATTGCTTGGACTTTAGATTCGTACCGTTTGACTAAAAATGTTTGTCCAAAATACTTTAGTGATTTTAAAGAGATGGAAAGAAACATCTTCATGATGGCTATGAAGCATGGGAAACCAACTAATGTTGGAGATTTGGAGAAACAAACTTTTTATAGGTTTATTCAGAAAACATATTTTCTTCATCCAATTTCTCTTGCTGTGCTGCTCTATTCTATTGGAGGAATCCCTTTTGTTATATGGGGAATGTGTGTGAGGATGAGTATTACATTTTGCTTTTTTTTCTCTCTCTCCTTAAGGTGGGTAGGTTTGCTTTCATTTGGAGAAGGATGGCATAATAACCACCATGCCTTTGAGTATTCAGCTAGACATGGACTTGAATGGTGGCAACTTGACTTTAGTTGGTATGTCATTATGTTCCTTCAAGCCATTGGAGTGGCCACTGAGGTAAAATTACCTTCTCAAAGTCACAAGGAAAGATTGGCTATGGACAAACTAAAAGAAGACATTCCAAGATGA

Coding sequence (CDS)

ATGGTGAAGCCTCTGATGTCATTTGGGAGGGGAGAATGGACCAATATAGACAAAACCATTGCATTGGTTCTTTTCTTTTTGCATGTGCCCTGTATTTTTGCACCATTTCAATTCACTTGGGGTGCATTTTGGGTTGCCTTCGTGTTATATGTTATCACAGGTCTTTCCGGTATTACTATTTCATATCATAGAAATCTTTCACACAAGAGTTTCAAGCTTCCCAAATGGCTCGAATATTTGTTTGCTTATTGTGGAGTTCATGCATTTCAGGGTGATCCGATCGATTGGGTGAGTACGCATAGGTGTCACCATCAATTTGTTGATACCGAAAAAGACCCGCACAGTCCTATTCATGGATTTTGGTTCAGTCATATTGCTTGGACTTTAGATTCGTACCGTTTGACTAAAAATGTTTGTCCAAAATACTTTAGTGATTTTAAAGAGATGGAAAGAAACATCTTCATGATGGCTATGAAGCATGGGAAACCAACTAATGTTGGAGATTTGGAGAAACAAACTTTTTATAGGTTTATTCAGAAAACATATTTTCTTCATCCAATTTCTCTTGCTGTGCTGCTCTATTCTATTGGAGGAATCCCTTTTGTTATATGGGGAATGTGTGTGAGGATGAGTATTACATTTTGCTTTTTTTTCTCTCTCTCCTTAAGGTGGGTAGGTTTGCTTTCATTTGGAGAAGGATGGCATAATAACCACCATGCCTTTGAGTATTCAGCTAGACATGGACTTGAATGGTGGCAACTTGACTTTAGTTGGTATGTCATTATGTTCCTTCAAGCCATTGGAGTGGCCACTGAGGTAAAATTACCTTCTCAAAGTCACAAGGAAAGATTGGCTATGGACAAACTAAAAGAAGACATTCCAAGATGA

Protein sequence

MVKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFFFSLSLRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLAMDKLKEDIPR
Homology
BLAST of Spg038881 vs. NCBI nr
Match: XP_038903746.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 520.8 bits (1340), Expect = 7.8e-144
Identity = 241/314 (76.75%), Postives = 266/314 (84.71%), Query Frame = 0

Query: 1   MVKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITI 60
           +VKPL  F RG+W NIDKT A V+FF+H+PCIF PFQFTWGAFWVAFVLYVITGL GITI
Sbjct: 11  LVKPLTPFERGKWNNIDKTTAFVIFFIHIPCIFGPFQFTWGAFWVAFVLYVITGLFGITI 70

Query: 61  SYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGF 120
           SYHR+LSHKSFKLPKWLEYLFAYCGVHA QGDP+DWVSTHRCHHQFVDTEKDPHSPI GF
Sbjct: 71  SYHRHLSHKSFKLPKWLEYLFAYCGVHALQGDPMDWVSTHRCHHQFVDTEKDPHSPIEGF 130

Query: 121 WFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQK 180
           WFSHIAWTLDSY LTK VCPKYF DFK MERN+ M+ +KHG+PTNV DLEKQ+FYRFIQK
Sbjct: 131 WFSHIAWTLDSYLLTKKVCPKYFRDFKGMERNMLMLNLKHGRPTNVVDLEKQSFYRFIQK 190

Query: 181 TYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFFF-------------------SLS 240
           TYFLHPI+LAV+LY+IGGIPFVIWGM VR+++     F                   S +
Sbjct: 191 TYFLHPIALAVVLYAIGGIPFVIWGMFVRVTMFVHVMFMVNSIGHRWGTRQWNTSDLSTN 250

Query: 241 LRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHK 296
             W+ LLSFG+GWHNNHHAFEYSARHGLEWWQ+DFSWYVIMFLQAIGVATEVKLP+QSHK
Sbjct: 251 NWWLSLLSFGDGWHNNHHAFEYSARHGLEWWQIDFSWYVIMFLQAIGVATEVKLPTQSHK 310

BLAST of Spg038881 vs. NCBI nr
Match: XP_038904662.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 471.5 bits (1212), Expect = 5.4e-129
Identity = 217/306 (70.92%), Postives = 243/306 (79.41%), Query Frame = 0

Query: 2   VKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITIS 61
           VKP MS  R EWTNIDKTIA +LF LH  CIFAPFQFTWGAFW+A VLYVITGL GITIS
Sbjct: 12  VKPTMSPRRREWTNIDKTIACLLFILHTLCIFAPFQFTWGAFWIALVLYVITGLFGITIS 71

Query: 62  YHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFW 121
           YHRNLSH+SFKLPKWLEYLFAYCG HA QGDPIDWVSTHRCHHQFVDT+ DPHSPI GFW
Sbjct: 72  YHRNLSHQSFKLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHQFVDTDNDPHSPIQGFW 131

Query: 122 FSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKT 181
           +SHI W LDSY LTK VC KYF+D KE ERN+F++ MKH +P NV DLEKQ FY FI KT
Sbjct: 132 YSHITWVLDSYNLTKKVCRKYFNDSKEAERNLFVLVMKHERPDNVKDLEKQIFYTFIHKT 191

Query: 182 YFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFFF-------------------SLSL 241
           Y LHPI+LAVLL  +GG+PFV+WGMCVR+ + +   F                   S + 
Sbjct: 192 YILHPIALAVLLSMVGGVPFVLWGMCVRIVMLYHVIFMVNSVCHKWGKQQWNTNDLSRNN 251

Query: 242 RWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKE 289
            WV  LSFGEGWHNNHHAFEYSA++GLEWWQLDF WYVIMFL+ IGVAT+VKLP+QSHK+
Sbjct: 252 WWVSFLSFGEGWHNNHHAFEYSAKYGLEWWQLDFGWYVIMFLKVIGVATDVKLPTQSHKQ 311

BLAST of Spg038881 vs. NCBI nr
Match: XP_011652979.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 455.3 bits (1170), Expect = 4.0e-124
Identity = 208/304 (68.42%), Postives = 237/304 (77.96%), Query Frame = 0

Query: 2   VKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITIS 61
           VKP+MS  + EWTNIDKTIA +LFFLH  CIFAPF FTW AFWVA +LY ITGL GITIS
Sbjct: 12  VKPIMSPTKREWTNIDKTIACLLFFLHALCIFAPFNFTWNAFWVALILYSITGLFGITIS 71

Query: 62  YHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFW 121
           YHRNLSHKSF+LPKWLEYLFAYCG HA QGDP+DWVSTHRCHHQFVDT+KDPH    GFW
Sbjct: 72  YHRNLSHKSFQLPKWLEYLFAYCGAHALQGDPMDWVSTHRCHHQFVDTDKDPHDRNQGFW 131

Query: 122 FSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKT 181
           FSHI W  DSY LTK VC KYF+D KE +RN+F + MKH +P NV DLEKQ FY FI KT
Sbjct: 132 FSHINWAFDSYHLTKKVCGKYFNDSKETKRNLFTLVMKHERPDNVKDLEKQIFYTFIHKT 191

Query: 182 YFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFFF-------------------SLSL 241
           Y LHPI+LA+ LY +GG+PFV+WGMCVR+ + +   F                   S + 
Sbjct: 192 YILHPIALAIFLYMVGGLPFVLWGMCVRIVMLYHVIFMVNSVCHKWGKKQWSTTDLSRNN 251

Query: 242 RWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKE 287
            WV LLSFGEGWHNNHHAFEYSA++GLEWWQLDF WYVIMFL+AIGVAT+VKLP+Q HK+
Sbjct: 252 WWVSLLSFGEGWHNNHHAFEYSAKYGLEWWQLDFGWYVIMFLKAIGVATDVKLPTQCHKQ 311

BLAST of Spg038881 vs. NCBI nr
Match: XP_022153632.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia] >XP_022153633.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 452.2 bits (1162), Expect = 3.4e-123
Identity = 206/313 (65.81%), Postives = 244/313 (77.96%), Query Frame = 0

Query: 1   MVKPLMSFG-RGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGIT 60
           M  P  SFG R +WT +DK +A  LFF+H+  +FAPF F W AFW+AF LYV TGL GI+
Sbjct: 31  MAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGLFGIS 90

Query: 61  ISYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHG 120
           +SYHRNL+HKSF LPKWLEYLFAYCG HA QGDPIDWV+THRCHH+FVDTE+DPHSPI G
Sbjct: 91  VSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHSPIQG 150

Query: 121 FWFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQ 180
           FWFSHI W LDSY LTK VCPKYF+DF+++ERNIF+M  KHG+P NVGDLEKQTFYRFI 
Sbjct: 151 FWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFYRFIH 210

Query: 181 KTYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITF---CFF----------------FSL 240
           KTYFLHPI LA++LY+ GG PF+IWGM VR+ +     C                   S 
Sbjct: 211 KTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWRTGDLSR 270

Query: 241 SLRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSH 294
           +  WV L+SFGEGWHNNHHAFEYSARHG+EWWQ+DF WYV+MFL+AIG+AT VK+PSQ+ 
Sbjct: 271 NNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVKVPSQNQ 330

BLAST of Spg038881 vs. NCBI nr
Match: XP_008454216.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 450.7 bits (1158), Expect = 9.9e-123
Identity = 210/308 (68.18%), Postives = 238/308 (77.27%), Query Frame = 0

Query: 1   MVKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITI 60
           MVKP+MS  R EWTNIDKTIA +LFF H  CIFAPF FTW AFWVA VLY +T L GITI
Sbjct: 11  MVKPIMSPTRREWTNIDKTIACLLFFFHALCIFAPFHFTWSAFWVAVVLYSVTSLFGITI 70

Query: 61  SYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGF 120
           SYHRNLSHKSFKLPKWLEYLFAYCGV AFQGDPIDWVSTHRCHHQFVDT+ DPH    GF
Sbjct: 71  SYHRNLSHKSFKLPKWLEYLFAYCGVQAFQGDPIDWVSTHRCHHQFVDTDNDPHDSNQGF 130

Query: 121 WFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQK 180
           WFSHI W LDSY LTK VC KYF++ KE +RN+F +  K  +P NV DLEKQ FY FI K
Sbjct: 131 WFSHITWLLDSYNLTKKVCGKYFNESKETKRNLFTLVTKLVRPDNVKDLEKQIFYTFIHK 190

Query: 181 TYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFF-------------------FSLS 240
           TY LHPI+LAVLLY +GG+PFV+WGMCVR+ I++                       S +
Sbjct: 191 TYLLHPIALAVLLYMVGGLPFVLWGMCVRIVISYHAISMVNSVCHKWGKKQWNTTDLSKN 250

Query: 241 LRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHK 290
             WV LLSFGEGWHNNHHAFEYSA++GLEWWQLDF WYVIMFL+AIGVAT+VKLP++SHK
Sbjct: 251 NWWVSLLSFGEGWHNNHHAFEYSAKYGLEWWQLDFGWYVIMFLKAIGVATDVKLPTESHK 310

BLAST of Spg038881 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 338.2 bits (866), Expect = 9.4e-92
Identity = 161/293 (54.95%), Postives = 204/293 (69.62%), Query Frame = 0

Query: 12  EWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSF 71
           EW  +D     V+  +H+  + APFQF W A  VAF LY++TGL GIT+S+HRNLSHK+F
Sbjct: 97  EWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAF 156

Query: 72  KLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDS 131
           KLPKWLEYLFAYCG  A QG+PIDWVSTHR HHQF D+++DPHSP+ GFWFSH+ W  D+
Sbjct: 157 KLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFDT 216

Query: 132 YRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAV 191
             +T+                      + G+P NVGDLEKQ FYRF++ TY LHP++LAV
Sbjct: 217 NTITQ----------------------RCGEPNNVGDLEKQPFYRFLRTTYILHPLALAV 276

Query: 192 LLYSIGGIPFVIWGMCVRM----SITF-----CFFF----------SLSLRWVGLLSFGE 251
            LY++GG PF++WGM VR+     IT+     C  +          S +  WV  L+FGE
Sbjct: 277 ALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGE 336

Query: 252 GWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLA 286
           GWHNNHHAFE+SARHGLEWWQLD +WYV+ FLQAIG+AT+VKLPS++ K+R+A
Sbjct: 337 GWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of Spg038881 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-79
Identity = 141/294 (47.96%), Postives = 187/294 (63.61%), Query Frame = 0

Query: 12  EWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSF 71
           +W  +D   A    F+H  C+ APF FTW A  VA ++Y + GL GIT+SYHRNL+H+SF
Sbjct: 33  KWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSYHRNLAHRSF 92

Query: 72  KLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDS 131
           K+PKWLEY FAYCG+ A QGDPIDWVSTHR HHQF D+++DPHSP  GFWFSH+ W  D+
Sbjct: 93  KVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDT 152

Query: 132 YRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAV 191
             L +                      K G+ TNV DL++Q +Y+F+Q+T   H ++   
Sbjct: 153 GYLVE----------------------KCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGF 212

Query: 192 LLYSIGGIPFVIWGMCVRMSI---TFCFFFSL----------------SLRWVGLLSFGE 251
           LLY  GG+ F+ WGM + +++     C   SL                ++ W+ + SFGE
Sbjct: 213 LLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGE 272

Query: 252 GWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLAM 287
            WHNNHHAFE SAR GLEWWQ+D SWY++ FL+ IG+AT+VKLPS+S + R+AM
Sbjct: 273 SWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAM 303

BLAST of Spg038881 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.1e-79
Identity = 140/293 (47.78%), Postives = 186/293 (63.48%), Query Frame = 0

Query: 12  EWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSF 71
           +W + D    L +  LHV C+FAP  F+W +FWV F LY I G+ G T+S+HRNL+H+SF
Sbjct: 39  KWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRSF 98

Query: 72  KLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDS 131
           KLPK+LEY FAY G+HA QGDP+ WVSTHR HH++ DT  DPHSPI GFWF HI W  DS
Sbjct: 99  KLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFDS 158

Query: 132 YRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAV 191
                    KY  +             K G+  N GDL KQ++YRF+++T+  H    A 
Sbjct: 159 ---------KYIIE-------------KCGRYENAGDLMKQSYYRFLERTFVYHVYLQAA 218

Query: 192 LLYSIGGIPFVIWGMCVRMSITFCFFF---SLSLRW----------------VGLLSFGE 251
           LLY  GG PF++WGM VR  + F   +   S+  RW                + +L+ GE
Sbjct: 219 LLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGE 278

Query: 252 GWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLA 286
           GWHNNHHAFEYSARHG+EWWQ+D +WY+I  L+ +G+AT++K+PS+ HK +++
Sbjct: 279 GWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMS 309

BLAST of Spg038881 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.0e-78
Identity = 141/299 (47.16%), Postives = 188/299 (62.88%), Query Frame = 0

Query: 13  WTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSFK 72
           W + D    +++  LHV C+ APF F+W AFW++  LY + G+ G T+SYHRNL+H+SFK
Sbjct: 37  WNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFK 96

Query: 73  LPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDSY 132
           LPK+LEY FAY G+HA QGDP+ WVSTHR HH+F DT  DPHSPI GFWFSHI W  DS 
Sbjct: 97  LPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDS- 156

Query: 133 RLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAVL 192
                   KY  +             + G+  N GDL KQ++YRF+++T+  H    A L
Sbjct: 157 --------KYILE-------------ECGRYENAGDLLKQSYYRFLERTFVFHVYLQAAL 216

Query: 193 LYSIGGIPFVIWGMCVRMSITF---------CFFF----------SLSLRWVGLLSFGEG 252
           LY  GG PF++WGM VR    F         C  +          S +  ++ +L+ GEG
Sbjct: 217 LYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEG 276

Query: 253 WHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLAMDKLKED 293
           WHNNHHAFEYSARHG+EWWQ+D +WYVI  L+ +G+AT+VK+PS+ HK +++     +D
Sbjct: 277 WHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHKRKMSFKNCVQD 313

BLAST of Spg038881 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.7e-77
Identity = 142/297 (47.81%), Postives = 186/297 (62.63%), Query Frame = 0

Query: 8   FGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLY-VITGLSGITISYHRNL 67
           F R +W   D   A+ L  LH     APF F+W AFW++F+L    +G+ GIT+ +HR L
Sbjct: 76  FWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCFHRCL 135

Query: 68  SHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIA 127
           +H  FKLPK +EY FAYCG  A QGDP++WVS HR HHQFVDTE+D HSP  GFWF HI 
Sbjct: 136 THGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWFCHIG 195

Query: 128 WTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHP 187
           W LD                    +++F +  + G+  NV DL+KQ FYRF+QKTY  H 
Sbjct: 196 WVLD--------------------KDLF-VEKRGGRRNNVNDLKKQAFYRFLQKTYMYHQ 255

Query: 188 ISLAVLLYSIGGIPFVIWGMCVRMSITFCFFFSLSL---RW----------------VGL 247
           ++L  LLY +GG P+++WGM  R+   F   F+++    +W                V L
Sbjct: 256 LALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNMFVAL 315

Query: 248 LSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERL 285
            +FGEGWHNNHHAFE SARHGLEWW++D +WYVI  LQAIG+AT VKLP+++ K++L
Sbjct: 316 CAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPTEAQKQKL 351

BLAST of Spg038881 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 206/313 (65.81%), Postives = 244/313 (77.96%), Query Frame = 0

Query: 1   MVKPLMSFG-RGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGIT 60
           M  P  SFG R +WT +DK +A  LFF+H+  +FAPF F W AFW+AF LYV TGL GI+
Sbjct: 31  MAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVATGLFGIS 90

Query: 61  ISYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHG 120
           +SYHRNL+HKSF LPKWLEYLFAYCG HA QGDPIDWV+THRCHH+FVDTE+DPHSPI G
Sbjct: 91  VSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDPHSPIQG 150

Query: 121 FWFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQ 180
           FWFSHI W LDSY LTK VCPKYF+DF+++ERNIF+M  KHG+P NVGDLEKQTFYRFI 
Sbjct: 151 FWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQTFYRFIH 210

Query: 181 KTYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITF---CFF----------------FSL 240
           KTYFLHPI LA++LY+ GG PF+IWGM VR+ +     C                   S 
Sbjct: 211 KTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWRTGDLSR 270

Query: 241 SLRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSH 294
           +  WV L+SFGEGWHNNHHAFEYSARHG+EWWQ+DF WYV+MFL+AIG+AT VK+PSQ+ 
Sbjct: 271 NNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVKVPSQNQ 330

BLAST of Spg038881 vs. ExPASy TrEMBL
Match: A0A1S3BZB5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103494691 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.8e-123
Identity = 210/308 (68.18%), Postives = 238/308 (77.27%), Query Frame = 0

Query: 1   MVKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITI 60
           MVKP+MS  R EWTNIDKTIA +LFF H  CIFAPF FTW AFWVA VLY +T L GITI
Sbjct: 11  MVKPIMSPTRREWTNIDKTIACLLFFFHALCIFAPFHFTWSAFWVAVVLYSVTSLFGITI 70

Query: 61  SYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGF 120
           SYHRNLSHKSFKLPKWLEYLFAYCGV AFQGDPIDWVSTHRCHHQFVDT+ DPH    GF
Sbjct: 71  SYHRNLSHKSFKLPKWLEYLFAYCGVQAFQGDPIDWVSTHRCHHQFVDTDNDPHDSNQGF 130

Query: 121 WFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQK 180
           WFSHI W LDSY LTK VC KYF++ KE +RN+F +  K  +P NV DLEKQ FY FI K
Sbjct: 131 WFSHITWLLDSYNLTKKVCGKYFNESKETKRNLFTLVTKLVRPDNVKDLEKQIFYTFIHK 190

Query: 181 TYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFF-------------------FSLS 240
           TY LHPI+LAVLLY +GG+PFV+WGMCVR+ I++                       S +
Sbjct: 191 TYLLHPIALAVLLYMVGGLPFVLWGMCVRIVISYHAISMVNSVCHKWGKKQWNTTDLSKN 250

Query: 241 LRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHK 290
             WV LLSFGEGWHNNHHAFEYSA++GLEWWQLDF WYVIMFL+AIGVAT+VKLP++SHK
Sbjct: 251 NWWVSLLSFGEGWHNNHHAFEYSAKYGLEWWQLDFGWYVIMFLKAIGVATDVKLPTESHK 310

BLAST of Spg038881 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.1e-122
Identity = 202/301 (67.11%), Postives = 235/301 (78.07%), Query Frame = 0

Query: 1   MVKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITI 60
           M+KP   + R +WT +DK IA  LF +H+ CI APFQF W AFWVAFVLY++TGL G+T+
Sbjct: 11  MMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVTGLFGMTL 70

Query: 61  SYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGF 120
           SYHRNL+HKSFKLPKWLEYLFAYCGV A QGDP+DWVSTHRCHHQFVDTE+DPHSPI GF
Sbjct: 71  SYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDPHSPIRGF 130

Query: 121 WFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQK 180
           WFSHI W  DSY LTK VCPKYFS+F+++ERNIF++  KHGKP NV DLEKQ FYRFI +
Sbjct: 131 WFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQAFYRFIHQ 190

Query: 181 TYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFF-------------------FSLS 240
           TY LHPI LAV+LY +GGIPF++WGM VR+ +   F                     S +
Sbjct: 191 TYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWTTGDMSKN 250

Query: 241 LRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHK 283
             WV L++FGEGWHNNHHAFEYSA+ GLEWWQLDF WY+I+FLQAIGVAT VKLPSQSH 
Sbjct: 251 NWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVKLPSQSHM 310

BLAST of Spg038881 vs. ExPASy TrEMBL
Match: A0A1S4E0J1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494694 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.6e-118
Identity = 206/314 (65.61%), Postives = 239/314 (76.11%), Query Frame = 0

Query: 1   MVK-PLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGIT 60
           MVK P+ SF R +WT +DK +A ++ F+H+ CIFAPFQF W AF VA  L VITGL G+T
Sbjct: 33  MVKPPVRSFWRRKWTTLDKRVAYIISFVHLLCIFAPFQFNWSAFRVAVALKVITGLFGMT 92

Query: 61  ISYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHG 120
           ISYHRNL+HKSF+LPKWLEYLFAYCGVHA QGDPIDWVSTHRCHHQFVDTEKDPHSPI+G
Sbjct: 93  ISYHRNLAHKSFQLPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTEKDPHSPING 152

Query: 121 FWFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQ 180
           FW SH+ W  DSY LT  VCPKY SD K++ERN+F++  KHGKP NVGDLEKQ FYRFI 
Sbjct: 153 FWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNVGDLEKQRFYRFIH 212

Query: 181 KTYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFFFSLSL----------------- 240
           KTY LH ++LA++LY++GG+PF IWGMCVR+ + F   F ++                  
Sbjct: 213 KTYMLHHLTLAIILYNVGGLPFFIWGMCVRIVVVFHSTFMVNSVCHIWGKQPWRTGDLSR 272

Query: 241 -RW-VGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSH 294
             W VGL SFGEGWHNNHHAF YSAR GLEWWQLD  WYVI FLQ IG+AT VKLPS SH
Sbjct: 273 NNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVIGLATNVKLPSPSH 332

BLAST of Spg038881 vs. ExPASy TrEMBL
Match: A0A1S3BZB9 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494694 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.6e-118
Identity = 206/314 (65.61%), Postives = 239/314 (76.11%), Query Frame = 0

Query: 1   MVK-PLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGIT 60
           MVK P+ SF R +WT +DK +A ++ F+H+ CIFAPFQF W AF VA  L VITGL G+T
Sbjct: 34  MVKPPVRSFWRRKWTTLDKRVAYIISFVHLLCIFAPFQFNWSAFRVAVALKVITGLFGMT 93

Query: 61  ISYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHG 120
           ISYHRNL+HKSF+LPKWLEYLFAYCGVHA QGDPIDWVSTHRCHHQFVDTEKDPHSPI+G
Sbjct: 94  ISYHRNLAHKSFQLPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTEKDPHSPING 153

Query: 121 FWFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQ 180
           FW SH+ W  DSY LT  VCPKY SD K++ERN+F++  KHGKP NVGDLEKQ FYRFI 
Sbjct: 154 FWISHMMWLFDSYTLTNKVCPKYSSDLKKIERNMFVIFTKHGKPDNVGDLEKQRFYRFIH 213

Query: 181 KTYFLHPISLAVLLYSIGGIPFVIWGMCVRMSITFCFFFSLSL----------------- 240
           KTY LH ++LA++LY++GG+PF IWGMCVR+ + F   F ++                  
Sbjct: 214 KTYMLHHLTLAIILYNVGGLPFFIWGMCVRIVVVFHSTFMVNSVCHIWGKQPWRTGDLSR 273

Query: 241 -RW-VGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSH 294
             W VGL SFGEGWHNNHHAF YSAR GLEWWQLD  WYVI FLQ IG+AT VKLPS SH
Sbjct: 274 NNWLVGLTSFGEGWHNNHHAFSYSARLGLEWWQLDAGWYVIKFLQVIGLATNVKLPSPSH 333

BLAST of Spg038881 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 338.2 bits (866), Expect = 6.7e-93
Identity = 161/293 (54.95%), Postives = 204/293 (69.62%), Query Frame = 0

Query: 12  EWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSF 71
           EW  +D     V+  +H+  + APFQF W A  VAF LY++TGL GIT+S+HRNLSHK+F
Sbjct: 97  EWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAF 156

Query: 72  KLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDS 131
           KLPKWLEYLFAYCG  A QG+PIDWVSTHR HHQF D+++DPHSP+ GFWFSH+ W  D+
Sbjct: 157 KLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFDT 216

Query: 132 YRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAV 191
             +T+                      + G+P NVGDLEKQ FYRF++ TY LHP++LAV
Sbjct: 217 NTITQ----------------------RCGEPNNVGDLEKQPFYRFLRTTYILHPLALAV 276

Query: 192 LLYSIGGIPFVIWGMCVRM----SITF-----CFFF----------SLSLRWVGLLSFGE 251
            LY++GG PF++WGM VR+     IT+     C  +          S +  WV  L+FGE
Sbjct: 277 ALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGE 336

Query: 252 GWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLA 286
           GWHNNHHAFE+SARHGLEWWQLD +WYV+ FLQAIG+AT+VKLPS++ K+R+A
Sbjct: 337 GWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of Spg038881 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 298.1 bits (762), Expect = 7.7e-81
Identity = 141/294 (47.96%), Postives = 187/294 (63.61%), Query Frame = 0

Query: 12  EWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSF 71
           +W  +D   A    F+H  C+ APF FTW A  VA ++Y + GL GIT+SYHRNL+H+SF
Sbjct: 33  KWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSYHRNLAHRSF 92

Query: 72  KLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDS 131
           K+PKWLEY FAYCG+ A QGDPIDWVSTHR HHQF D+++DPHSP  GFWFSH+ W  D+
Sbjct: 93  KVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDT 152

Query: 132 YRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAV 191
             L +                      K G+ TNV DL++Q +Y+F+Q+T   H ++   
Sbjct: 153 GYLVE----------------------KCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGF 212

Query: 192 LLYSIGGIPFVIWGMCVRMSI---TFCFFFSL----------------SLRWVGLLSFGE 251
           LLY  GG+ F+ WGM + +++     C   SL                ++ W+ + SFGE
Sbjct: 213 LLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGE 272

Query: 252 GWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLAM 287
            WHNNHHAFE SAR GLEWWQ+D SWY++ FL+ IG+AT+VKLPS+S + R+AM
Sbjct: 273 SWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAM 303

BLAST of Spg038881 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 289.7 bits (740), Expect = 2.7e-78
Identity = 139/293 (47.44%), Postives = 180/293 (61.43%), Query Frame = 0

Query: 13  WTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSFK 72
           W  +D       F +H   + APF FTW A WV F+ Y I GL GIT+SYHRNL+H+SFK
Sbjct: 36  WRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLAHRSFK 95

Query: 73  LPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDSY 132
           +PKWLEYL AYC + A QGDPIDWVSTHR HHQF D+E+DPHSP  GFWFSH+ W  DS 
Sbjct: 96  VPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSA 155

Query: 133 RLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAVL 192
            L                        K G+  NV DL++Q FYRF+QKT   H + L   
Sbjct: 156 YLVS----------------------KCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFF 215

Query: 193 LYSIGGIPFVIWGMCVRMSI---------TFCFFF----------SLSLRWVGLLSFGEG 252
           L+ +GG+ FV WGM V  ++         + C  +          S ++ W+ + SFGE 
Sbjct: 216 LFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGES 275

Query: 253 WHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLAM 287
           WHNNHHAFE SAR GLEWWQ+D SWY++ F + IG+AT+VK+P+++ + R+A+
Sbjct: 276 WHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAI 305

BLAST of Spg038881 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 285.0 bits (728), Expect = 6.7e-77
Identity = 143/305 (46.89%), Postives = 189/305 (61.97%), Query Frame = 0

Query: 1   MVKPLMSFGRGEWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITI 60
           +V+   SF    WT+ D +  ++    H+  + APF F+W AFWV   L  I G+  IT+
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 61  SYHRNLSHKSFKLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGF 120
           SYHRNLSH+SF LPKWLEYLFAY GV AFQGDPI+WVS HR HH+  +T++DPHSP  GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 121 WFSHIAWTLDSYRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQK 180
           WFSH+AW  D+  + +N                       G   NV DL +Q FYRF+Q+
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQR 258

Query: 181 TYFLHPISLAVLLYSIGGIPFVIWG----MCVRMSITF-----CFF----------FSLS 240
           T  LH ++ + L Y  GG+P ++WG    + VR+ +TF     C            FS +
Sbjct: 259 TVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKN 318

Query: 241 LRWVGLLSFGEGWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHK 287
             WV +L+ GEGWHNNHHAFE+SARHGLEWWQLD +W +I FL+AIG+AT VKLP+++  
Sbjct: 319 NWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQM 360

BLAST of Spg038881 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 264.6 bits (675), Expect = 9.4e-71
Identity = 130/294 (44.22%), Postives = 181/294 (61.56%), Query Frame = 0

Query: 12  EWTNIDKTIALVLFFLHVPCIFAPFQFTWGAFWVAFVLYVITGLSGITISYHRNLSHKSF 71
           +WT +D      +  +H+ C+ APF +TW AF  A ++ + T LS IT SYHRNL+H+SF
Sbjct: 27  KWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGISTNLS-ITFSYHRNLTHRSF 86

Query: 72  KLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQFVDTEKDPHSPIHGFWFSHIAWTLDS 131
           KLPKWLEY FAY  + A QG PIDWVSTHR HHQF D+++DPHSPI GFWFSH+ W  D+
Sbjct: 87  KLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWIFDT 146

Query: 132 YRLTKNVCPKYFSDFKEMERNIFMMAMKHGKPTNVGDLEKQTFYRFIQKTYFLHPISLAV 191
             + +                      K G   NV DL++Q FYRF+Q T  LH ++  +
Sbjct: 147 SYIRE----------------------KCGGRDNVMDLKQQWFYRFLQNTIGLHILTFWI 206

Query: 192 LLYSIGGIPFVIWGMCVRMSITF---------CFFF----------SLSLRWVGLLSFGE 251
           L+Y  GG+P++ W + V  +I +         C  +          S ++ W+G  + GE
Sbjct: 207 LVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWNTKDTSRNIWWLGPFTMGE 266

Query: 252 GWHNNHHAFEYSARHGLEWWQLDFSWYVIMFLQAIGVATEVKLPSQSHKERLAM 287
            WHNNHHAFE SARHGLEW+Q+D +WY+I F Q +G+AT+VKLP+ + K ++++
Sbjct: 267 SWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLATDVKLPTDAQKRKMSL 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903746.17.8e-14476.75palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_038904662.15.4e-12970.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_011652979.24.0e-12468.42palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic isoform... [more]
XP_022153632.13.4e-12365.81palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_008454216.19.9e-12368.18PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
Match NameE-valueIdentityDescription
Q949X09.4e-9254.95Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657971.1e-7947.96Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW24.1e-7947.78Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW32.0e-7847.16Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9FV681.7e-7747.81Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DHZ71.7e-12365.81palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A1S3BZB54.8e-12368.18palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DJP03.1e-12267.11palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A1S4E0J13.6e-11865.61palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is... [more]
A0A1S3BZB93.6e-11865.61palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is... [more]
Match NameE-valueIdentityDescription
AT3G15850.16.7e-9354.95fatty acid desaturase 5 [more]
AT1G06080.17.7e-8147.96delta 9 desaturase 1 [more]
AT2G31360.12.7e-7847.4416:0delta9 desaturase 2 [more]
AT3G15870.16.7e-7746.89Fatty acid desaturase family protein [more]
AT1G06120.19.4e-7144.22Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 231..245
score: 60.7
coord: 100..129
score: 52.11
coord: 63..83
score: 32.83
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 155..213
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 223..287
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 11..140
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 39..274
e-value: 7.88059E-55
score: 173.896
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 43..217
e-value: 1.2E-9
score: 38.4
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 155..213
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 11..140
coord: 223..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038881.1Spg038881.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water