Spg038852 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038852
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMethyltransferase
Locationscaffold12: 5313892 .. 5318511 (-)
RNA-Seq ExpressionSpg038852
SyntenySpg038852
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAATGTTAAACGTGGAGTATGCGCTGTCCTTCACGAGAACCAGAAGAGAGAGATTTTTTTTTGTGCTTGTTATCACTGCCCATTTCTTTTTCTTCCTTTTGTTTGTTGTTTGTTTGTTCGTCCGGCTTCAGCATTGATTACTCTGTAAAGATTCCTTTTTGAATTTTCATCCATTTTTACATTTGGGTTTGTTTCTCTCTCAGATCTTCTTGGGCTTTTGACGTAATACTCAACACGAATCACACCCATATTCTTATTCCCATTCCCTTTCCCCACTGCATTTTCTAACAAAAATGCTCTAACAAACCTTCGATTCTCTTTCTGATTCTTCTTTTGCTTCAGTTGTTCTCTTAGATCTCCCAGACCGCTTTTGATCACACCCATGTCGAAAGCTGGAGCGTTGGATCTTGCATCTGGCCTCGGCGGCAAGATAGACAAGGGCGAGGTCCTTTCCGCCGTTGAAAAGTAAACATTCCCTCAAATTTTCCACCCCAGATTGGTTTTTGTTTAGTTCATGGAACCCCATTTGTGAAATCATGTATATGGGTTGTATTGGTTGTGTTTTGGGCTATTCTGACAAGCTAATCGAGCTAGTTCATTCTCTTTTTGAGTCACTTACTTTGAATTCTATGTTTAAGCTTTAAGCCTCTGCATGTTCTGCTTTTTTGTTGTGTAATTCGGTTATGGCACCTTGATCTGTCGATATCAATTTTAGATATGTGGATGCTTTGAACGAAACAGGGCATTCTAGAATTGTGTACCATGTTGTGTGTAATGACAGGATTTCGTGAATTGAATGAAGATTTGGCCTGATTTGTTGTATTTGAATTGTGGTTTGTTGTATCTTTGTTTTTTTGAACTCATTGTGTTCTGAAAGTTTTGCATTGTTGAATCAGGTATGAGAAGTACCATGTCTGTTATGGAGGTGAAGAGGAGGAGAGGAAAGCCAATTACACTGACATGGTAAGAGAAGATTCCATTCTCATTGTGTTTCTTCTCAATATCTCCATGTAGTAGAATCACTCCTTGTCATTTTCATGCTTTCTTCACCATATTGGCTTTGGACTTAGCTGTGTAATATGTATTACTACAGGTTAACAAATACTATGATCTTGTTACGAGCTTTTACGAGTTCGGTTGGGGCGAGTCTTTCCATTTTGCACCTAGGTATGATGCTATCTCCCTTCTTCAGTTAACAGACATTCTATATGTCTCTGTTTTCTCAAATCTAAACGTGCATATTTTGCACTCTAGATGGAATGGTGAATCTCTGCGGGAGAGCATTAAGAGGCACGAGCACTTCCTTGCTTTGCAACTAGGTTTGAAACCTGGACAGAAGGTTCGTATTTTTCCATGCACATGGAGAAATTTTCTTCATTCATTTCAAATTATAGACAATAACTTTGTCTATATTCTAGGTGTTGGACGTTGGATGTGGAATTGGTGGACCACTTAGAGAAATTGCAAGATTTAGGTATGCTAAACTCCGTATCGTGCTACCTGCCCTGAAATGGAATCGAAATAAGTCTAACAACTAGATTGTTTACAGTCTCAATTGAATACTTCGATGCTGGATCTAGTATATATAGTTCTGTACTTTTTGTTTTATTTTTATATTTATGGTTGGTATCTCATATCCTATGACACTCTGTTGCAGTTCTACTTCAGTTACTGGATTGAACAACAATGAGTACCAGATTTCAAGGGGAAAGGTAATTTTTTTTAAATCTAATCCTTTGATGAAGGATGTCAGGAATATTGTTTCTTCGAAATATCTTGCTGTTGGTTGTAAATTTTCTTGACTTTTCTTTCTACTTTTTTCATTGCCTCGTTCATCATAATTACTATTCTGCTATTATTATGATGTATTCTGATCAAGTTCTTACAGTTAGATAATTGTATTTTTCGCTTTTTACTTCGTCAGGAACTGAATCGCGTTGCAAAAGTGGACAGGACTTGTGACTTTGTCAAGGTTGGTTTTTTGTAGATTACATTTAGTCTTTCAAAAATTTGTTTGTTCACAATTTTTATTTCATTGTGAACTTTTTTAGAAAAGAAAAGCCTACAATAGTTATACTTCAAAAGTTAATACTTATTCATTTTCTCCATGCTGCAGGCCGACTTCATGAAAATGCCATTTCCTGACAATTCATTCGATGCAGTGTATGCAATTGAAGCTACCTGTCACGCACCAGATGCAGTAAGTCTTTAATACGATATGTAGTGTTAATTTGAGTTTACAGCATTTATTGTTAATCTAATACGACTATTGTTTGCCGTGAGGTATGTGTTTTGGACATCTTGGTTTGACTATGTTTCTTGTCTGGCAGTATGGGTGCTACAAGGAGATTTATAGAGTACTAAAGCCTGGCCAACATTTTGCTGCTTATGAATGGTGTATGACTGATGCTTTTGATCCAAATAACCAAGAACATCAAAAGATAAAGGTAAGTCGGCTCCATCCCTTCTATTTCGTAGATTCTTCAATTGCACCGCACTGATTGATCCTATATGGATTACCTTGACCGAGTTCGAATTGTGCAGGCAGAAATTGAGATCGGTGATGGCCTTCCAGATATCAGGTTGACAGGAAAGTGCCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTTAGTTGGAAAAGAACTTCATATTATTAGTTTCCATTTTCATGAACAGATGAGAGACATCATTTATGGATTCAAAGCAAAGATTTCATATTCATGGGCACATGACACCAGGTTCTTTGGGAGAAAGATCTTGCTGTAAATTCGCCTCTTCCTTGGTACTTACCTTTAGACAAAAGCCATTTCTCATTGAGTAGCTTCCGTCTAACAGCAATTGGTCGTTTCATTACTAAAAATATGGTAAGCTTTATCAGATGTGACCTTGATGAGTTTTTTCCTCGATTATATTACGTCAAGGAGAACCTTTCTGTTTTACCAAACATATGGGAGACATTCAGGATGAAGTTAACAAGTGTTGAGTTGGATCTTTTTGTAGGTCAAAGCGCTAGAGTTCATTCGACTCGCCCCAAAGGGTAGCCAAAGAGTTCAAGACTTTCTAGAGAAAGCAGCCGAAGGGCTAGTTGCGGGTGGAAAGTGAGTATACCTTATTATGCTCTTGAAATTCTGTCTTTTACCTAACATGCTGGATTTGGTACCCTTTTTCTGAAAGTTCATTATTCCATTCTTGTTACATTAGATCCACATAATATTTTTGGGAAATGACCAAGAGGAGTGGAGCTAGTATTTTTCAAAGGGAGGGGAAAATTTTGCCTTTTAACCTTTTATATATAGTCGTTCAATTCCGAAAATCGAGACGGTAAATTAGAGATTAAACTTTAGAAAGATTTTAACTTTTTAATATAATTATTTTAATCATGATTTTGATATATAATTCCCTTGGCACCTTGATAGATCTTAGTCCATCCATCCATCTAATTTTAATTAGTAAAAGAAACATTCTCTGAAAGAATACAAAATCAATCCTACTCTTCCCCCATCATCTTCCTCCCTCTCGGAAAGAATCTACATCCGGGCTCCTTGAAGAAGGAACCTCGGAAAGCAAAATTCCTGTGGGAGACGGCGGGGTTTCTTCGGTATGCAATAATGCCAGCATGAGTTAGATTAAAAGAGAAGGGGGAGAGGAAGAAGTGTATGATTGAATGAGGAACTCTTTTTCTGGTAGATATGTTGATTCCATGTGTTCTTAAAGTTCTAACTTTAGAATGTTCTTCAAATAGAAATGCAATCGATAGCATGTCGTATCTCTCTCATATCTTAACTATGACGTTCGTTCTTTTAACTTACCCGAGTCGCTCATACGATGTTTCTTAATGTTCTCAGGAAAGAGATATTCACACCGATGTATTTCTTCTTGGCTCGGAAGCCGCTTTCAGACAGTGAGTAATTTGACAGTGCATAGTTAACTTATTTACTGCTACTCAGTTAGGGAGTGTTTGCAGTCATTCTGAAGAATGTTTTTTTGGCTTAATTTAACCATTCTAGAGCTTTTTTGTTTTTTCTTTTCCTTTTCCTTTTTGTTGTGGGGGGTAAAGTTGTCCTTTGCTTTTCTGAATTAGTTTCAAGTGGGTTATCTTTCTTCCTAGGAAGATGCTAAGTCTTAGCCTTTGTGCTTTCAATTTTTCTTGTTTGTTCTGTTAATGCAATAAATTAGTTTCTTTCTGATCTTATATGGGTTGTTCTTCTTTTGGAAATGTTGGTTTCCATTTGTTTTCTAACAAGGTTTACTGGCTCATTCTGTACTGCTTACTGTTTGTTGCGTCGGTATGCGATCGATGTTCCTGAAATACCATAGAAATGTTGACGAAATATAAAACGTGCATTGTTAGAGATTTCGACGAAAGTATCACCGTGATGAAATATAATTTGTTTAAGAGTATGAAATCCTAAGAAATGTTTGTCGATGTCGATATTTTAGTAACTATAGATAAGATATGAGATAATATCAAATCACATCCTTGTTTGTTTCAAATCTTTTTTTTTTTTTTTAATGATATCATTTTGGTTACTGTATTTTGGAACTTGTTCTATTTTAGTCTCTACTTCAAATGTTCGAAATTAATTCTCATACTTTCAATAAAGTTAGTCTCTAC

mRNA sequence

ATGACAATGTTAAACGTGGATTGTTCTCTTAGATCTCCCAGACCGCTTTTGATCACACCCATGTCGAAAGCTGGAGCGTTGGATCTTGCATCTGGCCTCGGCGGCAAGATAGACAAGGGCGAGGTCCTTTCCGCCGTTGAAAAATATGTGGATGCTTTGAACGAAACAGGGCATTCTAGAATTGTGTACCATGTTGTGTGTAATGACAGGATTTCGTATGAGAAGTACCATGTCTGTTATGGAGGTGAAGAGGAGGAGAGGAAAGCCAATTACACTGACATGGTTAACAAATACTATGATCTTGTTACGAGCTTTTACGAGTTCGGTTGGGGCGAGTCTTTCCATTTTGCACCTAGGTATGATGCTATCTCCCTTCTTCAGTTAACAGACATTCTATATGTCTCTGTTTTCTCAAATCTAAACGTGCATATTTTGCACTCTAGATGGAATGGTGAATCTCTGCGGGAGAGCATTAAGAGGCACGAGCACTTCCTTGCTTTGCAACTAGGTTTGAAACCTGGACAGAAGGTGTTGGACGTTGGATGTGGAATTGGTGGACCACTTAGAGAAATTGCAAGATTTAGTTCTACTTCAGTTACTGGATTGAACAACAATGAGTACCAGATTTCAAGGGGAAAGTTAGATAATTGTATTTTTCGCTTTTTACTTCGTCAGGAACTGAATCGCGTTGCAAAAGTGGACAGGACTTGTGACTTTGTCAAGGCAGAAATTGAGATCGGTGATGGCCTTCCAGATATCAGGTTGACAGGAAAGTGCCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTTCTTTGGGAGAAAGATCTTGCTGTAAATTCGCCTCTTCCTTGGTACTTACCTTTAGACAAAAGCCATTTCTCATTGAGTAGCTTCCGTCTAACAGCAATTGGTCGTTTCATTACTAAAAATATGGTCAAAGCGCTAGAGTTCATTCGACTCGCCCCAAAGGGTAGCCAAAGAGTTCAAGACTTTCTAGAGAAAGCAGCCGAAGGGCTAGTTGCGGGTGGAAAGAAAGAGATATTCACACCGATGTATTTCTTCTTGGCTCGGAAGCCGCTTTCAGACAGTGAGTAA

Coding sequence (CDS)

ATGACAATGTTAAACGTGGATTGTTCTCTTAGATCTCCCAGACCGCTTTTGATCACACCCATGTCGAAAGCTGGAGCGTTGGATCTTGCATCTGGCCTCGGCGGCAAGATAGACAAGGGCGAGGTCCTTTCCGCCGTTGAAAAATATGTGGATGCTTTGAACGAAACAGGGCATTCTAGAATTGTGTACCATGTTGTGTGTAATGACAGGATTTCGTATGAGAAGTACCATGTCTGTTATGGAGGTGAAGAGGAGGAGAGGAAAGCCAATTACACTGACATGGTTAACAAATACTATGATCTTGTTACGAGCTTTTACGAGTTCGGTTGGGGCGAGTCTTTCCATTTTGCACCTAGGTATGATGCTATCTCCCTTCTTCAGTTAACAGACATTCTATATGTCTCTGTTTTCTCAAATCTAAACGTGCATATTTTGCACTCTAGATGGAATGGTGAATCTCTGCGGGAGAGCATTAAGAGGCACGAGCACTTCCTTGCTTTGCAACTAGGTTTGAAACCTGGACAGAAGGTGTTGGACGTTGGATGTGGAATTGGTGGACCACTTAGAGAAATTGCAAGATTTAGTTCTACTTCAGTTACTGGATTGAACAACAATGAGTACCAGATTTCAAGGGGAAAGTTAGATAATTGTATTTTTCGCTTTTTACTTCGTCAGGAACTGAATCGCGTTGCAAAAGTGGACAGGACTTGTGACTTTGTCAAGGCAGAAATTGAGATCGGTGATGGCCTTCCAGATATCAGGTTGACAGGAAAGTGCCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTTCTTTGGGAGAAAGATCTTGCTGTAAATTCGCCTCTTCCTTGGTACTTACCTTTAGACAAAAGCCATTTCTCATTGAGTAGCTTCCGTCTAACAGCAATTGGTCGTTTCATTACTAAAAATATGGTCAAAGCGCTAGAGTTCATTCGACTCGCCCCAAAGGGTAGCCAAAGAGTTCAAGACTTTCTAGAGAAAGCAGCCGAAGGGCTAGTTGCGGGTGGAAAGAAAGAGATATTCACACCGATGTATTTCTTCTTGGCTCGGAAGCCGCTTTCAGACAGTGAGTAA

Protein sequence

MTMLNVDCSLRSPRPLLITPMSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKAEIEIGDGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRLAPKGSQRVQDFLEKAAEGLVAGGKKEIFTPMYFFLARKPLSDSE
Homology
[Close]
BLAST of Spg038852 vs. NCBI nr
Match: KAG6606886.1 (Cycloartenol-C-24-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.9 bits (1265), Expect = 4.8e-135
Identity = 272/417 (65.23%), Postives = 279/417 (66.91%), Query Frame = 0 

Query: 15  PLLITPMSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYE 74
           PLLIT MSKAGALDLASGLGGKIDK EVLSAVEK                        YE
Sbjct: 77  PLLITLMSKAGALDLASGLGGKIDKNEVLSAVEK------------------------YE 136

Query: 75  KYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYV 134
           KYHVCYGG+E ERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP                
Sbjct: 137 KYHVCYGGDETERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP---------------- 196

Query: 135 SVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARF 194
                        RWNGESLRESIKRHEHFLALQL LKPGQKVLDVGCGIGGPLREIARF
Sbjct: 197 -------------RWNGESLRESIKRHEHFLALQLNLKPGQKVLDVGCGIGGPLREIARF 256

Query: 195 SSTSVTGLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFV-------------- 254
           SSTSVTGLNNNEYQISRGK            ELNR+AKVD+TCDFV              
Sbjct: 257 SSTSVTGLNNNEYQISRGK------------ELNRIAKVDKTCDFVKADFMKMPFPDNSF 316

Query: 255 -----------------------------------------------------KAEIEIG 314
                                                                KAEIEIG
Sbjct: 317 DAVYAIEATCHAPDAYGCYKEIYRVLKPGQHFAAYEWCMADAYDPNNQEHQKIKAEIEIG 376

Query: 315 DGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFI 365
           DGLPDIRLTGKCLEALKQAGFEV+WEKDLAVNSP+PWYLPLDKSHFSLSSFRLTAIGRFI
Sbjct: 377 DGLPDIRLTGKCLEALKQAGFEVVWEKDLAVNSPVPWYLPLDKSHFSLSSFRLTAIGRFI 428

[Close]
BLAST of Spg038852 vs. NCBI nr
Match: KAG6581566.1 (Cycloartenol-C-24-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.1 bits (1263), Expect = 8.2e-135
Identity = 272/420 (64.76%), Postives = 278/420 (66.19%), Query Frame = 0 

Query: 12  SPRPLLITPMSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRI 71
           S  PLLI PMSKAGALDLASGLGGK+DK EVLSAVEK                       
Sbjct: 530 SHTPLLIIPMSKAGALDLASGLGGKLDKNEVLSAVEK----------------------- 589

Query: 72  SYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDI 131
            YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP             
Sbjct: 590 -YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP------------- 649

Query: 132 LYVSVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREI 191
                           RWNGESLRESIKRHEHFLALQL LKPG KVLDVGCGIGGPLREI
Sbjct: 650 ----------------RWNGESLRESIKRHEHFLALQLDLKPGHKVLDVGCGIGGPLREI 709

Query: 192 ARFSSTSVTGLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFV----------- 251
           ARFS TSVTGLNNNEYQISRGK            ELNRVAKVD+TCDFV           
Sbjct: 710 ARFSYTSVTGLNNNEYQISRGK------------ELNRVAKVDKTCDFVKADFMKMPFPD 769

Query: 252 --------------------------------------------------------KAEI 311
                                                                   KAEI
Sbjct: 770 NSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDSNNQEHQKIKAEI 829

Query: 312 EIGDGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIG 365
           EIGDGLPDIRLTGKCLEALKQAGFEV+WE+DLAVNSP+PWYLPLDKSHFSLSSFRLTAIG
Sbjct: 830 EIGDGLPDIRLTGKCLEALKQAGFEVVWERDLAVNSPVPWYLPLDKSHFSLSSFRLTAIG 884

[Close]
BLAST of Spg038852 vs. NCBI nr
Match: XP_023526267.1 (cycloartenol-C-24-methyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526269.1 cycloartenol-C-24-methyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.1 bits (1263), Expect = 8.2e-135
Identity = 272/420 (64.76%), Postives = 278/420 (66.19%), Query Frame = 0 

Query: 12  SPRPLLITPMSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRI 71
           S  PLLI PMSKAGALDLASGLGGK+DK EVLSAVEK                       
Sbjct: 17  SHTPLLIIPMSKAGALDLASGLGGKLDKNEVLSAVEK----------------------- 76

Query: 72  SYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDI 131
            YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP             
Sbjct: 77  -YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP------------- 136

Query: 132 LYVSVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREI 191
                           RWNGESLRESIKRHEHFLALQL LKPG KVLDVGCGIGGPLREI
Sbjct: 137 ----------------RWNGESLRESIKRHEHFLALQLDLKPGHKVLDVGCGIGGPLREI 196

Query: 192 ARFSSTSVTGLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFV----------- 251
           ARFS TSVTGLNNNEYQISRGK            ELNRVAKVD+TCDFV           
Sbjct: 197 ARFSYTSVTGLNNNEYQISRGK------------ELNRVAKVDKTCDFVKADFMKMPFPD 256

Query: 252 --------------------------------------------------------KAEI 311
                                                                   KAEI
Sbjct: 257 NSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDSNNQEHQKIKAEI 316

Query: 312 EIGDGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIG 365
           EIGDGLPDIRLTGKCLEALKQAGFEV+WE+DLAVNSP+PWYLPLDKSHFSLSSFRLTAIG
Sbjct: 317 EIGDGLPDIRLTGKCLEALKQAGFEVVWERDLAVNSPVPWYLPLDKSHFSLSSFRLTAIG 371

[Close]
BLAST of Spg038852 vs. NCBI nr
Match: XP_022935654.1 (cycloartenol-C-24-methyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 491.1 bits (1263), Expect = 8.2e-135
Identity = 272/420 (64.76%), Postives = 278/420 (66.19%), Query Frame = 0 

Query: 12  SPRPLLITPMSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRI 71
           S  PLLI PMSKAGALDLASGLGGK+DK EVLSAVEK                       
Sbjct: 33  SHTPLLIIPMSKAGALDLASGLGGKLDKNEVLSAVEK----------------------- 92

Query: 72  SYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDI 131
            YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP             
Sbjct: 93  -YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP------------- 152

Query: 132 LYVSVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREI 191
                           RWNGESLRESIKRHEHFLALQL LKPG KVLDVGCGIGGPLREI
Sbjct: 153 ----------------RWNGESLRESIKRHEHFLALQLDLKPGHKVLDVGCGIGGPLREI 212

Query: 192 ARFSSTSVTGLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFV----------- 251
           ARFS TSVTGLNNNEYQISRGK            ELNRVAKVD+TCDFV           
Sbjct: 213 ARFSYTSVTGLNNNEYQISRGK------------ELNRVAKVDKTCDFVKADFMKMPFPD 272

Query: 252 --------------------------------------------------------KAEI 311
                                                                   KAEI
Sbjct: 273 NSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDSNNQEHQKIKAEI 332

Query: 312 EIGDGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIG 365
           EIGDGLPDIRLTGKCLEALKQAGFEV+WE+DLAVNSP+PWYLPLDKSHFSLSSFRLTAIG
Sbjct: 333 EIGDGLPDIRLTGKCLEALKQAGFEVVWERDLAVNSPVPWYLPLDKSHFSLSSFRLTAIG 387

[Close]
BLAST of Spg038852 vs. NCBI nr
Match: KHG15107.1 (Cycloartenol-C-24-methyltransferase -like protein [Gossypium arboreum])

HSP 1 Score: 490.3 bits (1261), Expect = 1.4e-134
Identity = 251/344 (72.97%), Postives = 271/344 (78.78%), Query Frame = 0 

Query: 21  MSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCY 80
           MSKAGALDLASGLGGKI+K EVLSAVE+                        YE+YHV Y
Sbjct: 1   MSKAGALDLASGLGGKIEKAEVLSAVEQ------------------------YERYHVFY 60

Query: 81  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNL 140
           GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP                      
Sbjct: 61  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP---------------------- 120

Query: 141 NVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 200
                  RWNGESL+ESIKRHEHFLALQLGLKPG KVLDVGCGIGGPLREIARFSSTSVT
Sbjct: 121 -------RWNGESLKESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSSTSVT 180

Query: 201 GLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKAEIEIGDGLPDIRLTGKCL 260
           G+NNNEYQI+RGK            ELNR+A V++TC+FVKAEIEIGDGLPDIRLTG+CL
Sbjct: 181 GINNNEYQITRGK------------ELNRIAGVEKTCNFVKAEIEIGDGLPDIRLTGQCL 240

Query: 261 EALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRL 320
           EALKQAGFE++WEKDLAV SPLPWYLPLD SHFSLSSFRLT++GRFIT+NMVKALEF+ L
Sbjct: 241 EALKQAGFEIIWEKDLAVGSPLPWYLPLDTSHFSLSSFRLTSVGRFITRNMVKALEFVGL 279

Query: 321 APKGSQRVQDFLEKAAEGLVAGGKKEIFTPMYFFLARKPLSDSE 365
           APKGSQRVQDFLEKAAEGLV GG+KEIFTPMYFFLARKPLS+S+
Sbjct: 301 APKGSQRVQDFLEKAAEGLVEGGRKEIFTPMYFFLARKPLSESQ 279

[Close]
BLAST of Spg038852 vs. ExPASy Swiss-Prot
Match: Q9LM02 (Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=SMT1 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.6e-115
Identity = 229/400 (57.25%), Postives = 254/400 (63.50%), Query Frame = 0 

Query: 27  LDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCYGGEEEE 86
           +DLAS LGGKIDK +VL+AVEK                        YE+YHV +GG EEE
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEK------------------------YEQYHVFHGGNEEE 60

Query: 87  RKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNVHILH 146
           RKANYTDMVNKYYDL TSFYE+GWGESFHFA                             
Sbjct: 61  RKANYTDMVNKYYDLATSFYEYGWGESFHFA----------------------------- 120

Query: 147 SRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNE 206
            RW GESLRESIKRHEHFLALQLG++PGQKVLDVGCGIGGPLREIARFS++ VTGLNNNE
Sbjct: 121 QRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNNE 180

Query: 207 YQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKA------------------------ 266
           YQI+RGK            ELNR+A VD+TC+FVKA                        
Sbjct: 181 YQITRGK------------ELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHA 240

Query: 267 -------------------------------------------EIEIGDGLPDIRLTGKC 326
                                                      EIEIGDGLPDIRLT KC
Sbjct: 241 PDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKC 300

Query: 327 LEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIR 360
           LEALKQAGFEV+WEKDLA +SP+PWYLPLDK+HFSLSSFRLTA+GRFITKNMVK LE+IR
Sbjct: 301 LEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIR 335

[Close]
BLAST of Spg038852 vs. ExPASy Swiss-Prot
Match: Q6ZIX2 (Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Smt1-1 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.2e-109
Identity = 221/409 (54.03%), Postives = 249/409 (60.88%), Query Frame = 0 

Query: 21  MSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCY 80
           MS++GA+DLASGLGGKI K EV SAV++                        YEKYH  Y
Sbjct: 1   MSRSGAMDLASGLGGKITKDEVKSAVDE------------------------YEKYHGYY 60

Query: 81  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNL 140
           GG+EE RK+NYTDMVNKYYDL TSFYE+GWGESFHFA                       
Sbjct: 61  GGKEEARKSNYTDMVNKYYDLATSFYEYGWGESFHFA----------------------- 120

Query: 141 NVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 200
                  RWNGESLRESIKRHEHFLALQLG+KPG KVLDVGCGIGGPLREIA+FS  SVT
Sbjct: 121 ------HRWNGESLRESIKRHEHFLALQLGVKPGMKVLDVGCGIGGPLREIAKFSLASVT 180

Query: 201 GLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKA------------------ 260
           GLNNNEYQI+RGK            ELNRVA V  TCDFVKA                  
Sbjct: 181 GLNNNEYQITRGK------------ELNRVAGVSGTCDFVKADFMKMPFSDNTFDAVYAI 240

Query: 261 -------------------------------------------------EIEIGDGLPDI 320
                                                            EIE+G+GLPDI
Sbjct: 241 EATCHAPDPVGCYKEIYRVLKPGQCFAVYEWCITDHYEPNNATHKRIKDEIELGNGLPDI 300

Query: 321 RLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVK 363
           R T +CL+A K AGFEV+W+KDLA +SP+PWYLPLD S FSLSSFRLT +GR IT+ MVK
Sbjct: 301 RSTQQCLQAAKDAGFEVIWDKDLAEDSPVPWYLPLDPSRFSLSSFRLTTVGRAITRTMVK 344

[Close]
BLAST of Spg038852 vs. ExPASy Swiss-Prot
Match: Q54I98 (Probable cycloartenol-C-24-methyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=smt1 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 5.4e-57
Identity = 135/359 (37.60%), Postives = 179/359 (49.86%), Query Frame = 0 

Query: 68  NDRI--SYEKYHVCYGGEEEE----RKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYD 127
           ND++  +   Y   + G +++    RK NYT MVN +YDL T FYEFGWG+SFHFA R+ 
Sbjct: 29  NDKVDDTLNGYKELFKGNDDKAIQARKNNYTHMVNTFYDLATDFYEFGWGQSFHFATRH- 88

Query: 128 AISLLQLTDILYVSVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVG 187
                                         ES   SI RHE ++A QLGL PG KV+D+G
Sbjct: 89  ----------------------------KYESFEASIARHEMYMAHQLGLFPGMKVIDIG 148

Query: 188 CGIGGPLREIARFSSTSVTGLNNNEYQISRGKLDN--------CIFRFLLRQELNRVAKV 247
           CG+GGP+R IARFS  +V GLNNNEYQI RGK  N        C F   ++ +   V   
Sbjct: 149 CGVGGPMRTIARFSGANVVGLNNNEYQIQRGKRLNESAGLSHLCSF---IKADFMHVPVE 208

Query: 248 DRTCDF--------------------------------------------------VKAE 307
           D T D                                                   +K +
Sbjct: 209 DNTYDCAYQIEATCHAPDLVGLYKEVFRIVKPGGLFGGYEWIMTNKFNPEDPVEVNIKKQ 268

Query: 308 IEIGDGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAI 363
           IE+G+GLPD+    + + A K AGFEV+   D+A  S LPWYLPL  S  S++ F  T +
Sbjct: 269 IELGNGLPDLVKPAEIINAAKAAGFEVITAFDVAETSELPWYLPL-SSGVSITGFLHTGV 328

[Close]
BLAST of Spg038852 vs. ExPASy Swiss-Prot
Match: Q6C2D9 (Sterol 24-C-methyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ERG6 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.7e-48
Identity = 119/340 (35.00%), Postives = 174/340 (51.18%), Query Frame = 0 

Query: 83  EEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNV 142
           +E+ R  +Y  +   YY+LVT FYE+GWG SFHF+ RY                      
Sbjct: 67  DEQGRIEDYAGLTKHYYNLVTDFYEYGWGSSFHFS-RY---------------------- 126

Query: 143 HILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGL 202
                 + GE+ R++  RHEH+LA ++G++PG KVLDVGCG+GGP REIARF+  ++ GL
Sbjct: 127 ------YKGEAFRQATARHEHYLAYKMGIQPGMKVLDVGCGVGGPAREIARFTGANIVGL 186

Query: 203 NNNEYQISRGK------------------------LDNC--------------------- 262
           NNN+YQ+ RG                          DN                      
Sbjct: 187 NNNDYQVERGTHYSEVQGFGDQVTYVKGDFMQMDFPDNSFDAVYAIEATVHAPVLEGVYS 246

Query: 263 -IFRFLLRQELNRVAKVDRTCDFVKAE---------IEIGDGLPDIRLTGKCLEALKQAG 322
            IFR L    +  V +   T ++ ++          IE GDG+P +      ++AL+  G
Sbjct: 247 EIFRVLKPGGVFGVYEWVMTDEYDESNPEHRDICYGIEKGDGIPKMYKREVAVKALENVG 306

Query: 323 FEVLWEKDLAV-NSPLPWYLPLDKSHFSLSSF-------RLTAIGRFITKNMVKALEFIR 360
           F++ +++DLA  ++ +PWY PL      + S        R + +GR +T N++ ALE I 
Sbjct: 307 FDIEYQEDLAADDAEVPWYYPLAGEWKYVQSLNDIVTIGRTSRLGRMVTMNVIGALEKIG 366

[Close]
BLAST of Spg038852 vs. ExPASy Swiss-Prot
Match: Q759S7 (Sterol 24-C-methyltransferase OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=ERG6 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.0e-47
Identity = 121/339 (35.69%), Postives = 166/339 (48.97%), Query Frame = 0 

Query: 84  EEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNVH 143
           EE R A+Y +  + YY++VT FYE+GWG SFHF+  Y                       
Sbjct: 60  EERRLADYNESTHSYYNVVTDFYEYGWGASFHFSRFY----------------------- 119

Query: 144 ILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLN 203
                  GES   S+ RHEH+LA + G+  G  VLDVGCG+GGP REIARF+   V GLN
Sbjct: 120 ------TGESFAMSMARHEHYLAHRAGITSGDLVLDVGCGVGGPAREIARFTGCRVVGLN 179

Query: 204 NNEYQISRGK---------------LDNCIFRFLLRQELNRVAKVDRTC----------- 263
           NN+YQI +GK                 + +         ++V  ++ TC           
Sbjct: 180 NNDYQIMKGKHYSRKLGLGDQVSYVKGDFMNMDFPDATFDKVYAIEATCHAPSFEGVYGE 239

Query: 264 --------------DFVKAE---------------IEIGDGLPDIRLTGKCLEALKQAGF 323
                         ++V  E               IE+GDG+P +       +AL + GF
Sbjct: 240 IYRVLKPGGVFAVYEWVMTENYDETNPEHRRIAYDIELGDGIPKMYSVKVARDALAKVGF 299

Query: 324 EVLWEKDLAVN-SPLPWYLPLD------KSHFSLSSF-RLTAIGRFITKNMVKALEFIRL 360
           E+L ++D A N  P+PWY PL       ++   L +F R +A+GR +T   V  +E +RL
Sbjct: 300 EILVDEDRADNDDPVPWYYPLTGEWAYIQTIADLGTFVRTSALGRRVTSAFVSLMETLRL 359

[Close]
BLAST of Spg038852 vs. ExPASy TrEMBL
Match: A0A0A0LD12 (SAM_MT_ERG6_SMT domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G689270 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.2e-139
Identity = 264/344 (76.74%), Postives = 270/344 (78.49%), Query Frame = 0 

Query: 21  MSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCY 80
           MSK GALDLASGLGGK++K EVLSAVEK                        YEKYHVCY
Sbjct: 1   MSKTGALDLASGLGGKLEKNEVLSAVEK------------------------YEKYHVCY 60

Query: 81  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNL 140
           GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP                      
Sbjct: 61  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP---------------------- 120

Query: 141 NVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 200
                  RW GESLRESIKRHEHFLALQL LKPG KVLDVGCGIGGPLREIARFS TSVT
Sbjct: 121 -------RWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIARFSYTSVT 180

Query: 201 GLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKAEIEIGDGLPDIRLTGKCL 260
           GLNNNEYQISRGK            ELNRVAKVDRTCDFVKAEIEIGDGLPDIR+TGKCL
Sbjct: 181 GLNNNEYQISRGK------------ELNRVAKVDRTCDFVKAEIEIGDGLPDIRMTGKCL 240

Query: 261 EALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRL 320
           EALKQAGFEV+WEKDLA NSPLPWYLPLDKSHFSLSSFRLTA+GRFITKNMVKALEFIRL
Sbjct: 241 EALKQAGFEVIWEKDLAENSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFIRL 279

Query: 321 APKGSQRVQDFLEKAAEGLVAGGKKEIFTPMYFFLARKPLSDSE 365
           APKGSQRVQDFLEKAAEGLV GGKKEIFTPMYFFLARKPLS +E
Sbjct: 301 APKGSQRVQDFLEKAAEGLVEGGKKEIFTPMYFFLARKPLSATE 279

[Close]
BLAST of Spg038852 vs. ExPASy TrEMBL
Match: A0A6J1F616 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442447 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 4.0e-135
Identity = 272/420 (64.76%), Postives = 278/420 (66.19%), Query Frame = 0 

Query: 12  SPRPLLITPMSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRI 71
           S  PLLI PMSKAGALDLASGLGGK+DK EVLSAVEK                       
Sbjct: 33  SHTPLLIIPMSKAGALDLASGLGGKLDKNEVLSAVEK----------------------- 92

Query: 72  SYEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDI 131
            YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP             
Sbjct: 93  -YEKYHVCYGGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP------------- 152

Query: 132 LYVSVFSNLNVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREI 191
                           RWNGESLRESIKRHEHFLALQL LKPG KVLDVGCGIGGPLREI
Sbjct: 153 ----------------RWNGESLRESIKRHEHFLALQLDLKPGHKVLDVGCGIGGPLREI 212

Query: 192 ARFSSTSVTGLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFV----------- 251
           ARFS TSVTGLNNNEYQISRGK            ELNRVAKVD+TCDFV           
Sbjct: 213 ARFSYTSVTGLNNNEYQISRGK------------ELNRVAKVDKTCDFVKADFMKMPFPD 272

Query: 252 --------------------------------------------------------KAEI 311
                                                                   KAEI
Sbjct: 273 NSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDSNNQEHQKIKAEI 332

Query: 312 EIGDGLPDIRLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIG 365
           EIGDGLPDIRLTGKCLEALKQAGFEV+WE+DLAVNSP+PWYLPLDKSHFSLSSFRLTAIG
Sbjct: 333 EIGDGLPDIRLTGKCLEALKQAGFEVVWERDLAVNSPVPWYLPLDKSHFSLSSFRLTAIG 387

[Close]
BLAST of Spg038852 vs. ExPASy TrEMBL
Match: A0A0B0NR21 (Cycloartenol-C-24-methyltransferase-like protein OS=Gossypium arboreum OX=29729 GN=F383_19071 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 6.7e-135
Identity = 251/344 (72.97%), Postives = 271/344 (78.78%), Query Frame = 0 

Query: 21  MSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCY 80
           MSKAGALDLASGLGGKI+K EVLSAVE+                        YE+YHV Y
Sbjct: 1   MSKAGALDLASGLGGKIEKAEVLSAVEQ------------------------YERYHVFY 60

Query: 81  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNL 140
           GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP                      
Sbjct: 61  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAP---------------------- 120

Query: 141 NVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 200
                  RWNGESL+ESIKRHEHFLALQLGLKPG KVLDVGCGIGGPLREIARFSSTSVT
Sbjct: 121 -------RWNGESLKESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIARFSSTSVT 180

Query: 201 GLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKAEIEIGDGLPDIRLTGKCL 260
           G+NNNEYQI+RGK            ELNR+A V++TC+FVKAEIEIGDGLPDIRLTG+CL
Sbjct: 181 GINNNEYQITRGK------------ELNRIAGVEKTCNFVKAEIEIGDGLPDIRLTGQCL 240

Query: 261 EALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRL 320
           EALKQAGFE++WEKDLAV SPLPWYLPLD SHFSLSSFRLT++GRFIT+NMVKALEF+ L
Sbjct: 241 EALKQAGFEIIWEKDLAVGSPLPWYLPLDTSHFSLSSFRLTSVGRFITRNMVKALEFVGL 279

Query: 321 APKGSQRVQDFLEKAAEGLVAGGKKEIFTPMYFFLARKPLSDSE 365
           APKGSQRVQDFLEKAAEGLV GG+KEIFTPMYFFLARKPLS+S+
Sbjct: 301 APKGSQRVQDFLEKAAEGLVEGGRKEIFTPMYFFLARKPLSESQ 279

[Close]
BLAST of Spg038852 vs. ExPASy TrEMBL
Match: A0A6J1DGP7 (Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020326 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.7e-133
Identity = 269/411 (65.45%), Postives = 274/411 (66.67%), Query Frame = 0 

Query: 21  MSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCY 80
           MSKAGALDLASGLGGK+DKGEVLSAVEK                        YEKYHVCY
Sbjct: 1   MSKAGALDLASGLGGKLDKGEVLSAVEK------------------------YEKYHVCY 60

Query: 81  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNL 140
           GGEEEERKANY+DMVNKYYDLVTSFYEFGWGESFHFAP                      
Sbjct: 61  GGEEEERKANYSDMVNKYYDLVTSFYEFGWGESFHFAP---------------------- 120

Query: 141 NVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 200
                  RWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT
Sbjct: 121 -------RWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 180

Query: 201 GLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFV-------------------- 260
           GLNNNEYQISRGK            ELNRVAKV  TCDFV                    
Sbjct: 181 GLNNNEYQISRGK------------ELNRVAKVHNTCDFVKADFMKMPFPDNSFDAVYAI 240

Query: 261 -----------------------------------------------KAEIEIGDGLPDI 320
                                                          KAEIEIGDGLPDI
Sbjct: 241 EATCHAPDAYGCYKEIYRVLKPGQHFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDI 300

Query: 321 RLTGKCLEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVK 365
           R TGKC+EALKQAGFEV+WEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVK
Sbjct: 301 RSTGKCIEALKQAGFEVIWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVK 346

[Close]
BLAST of Spg038852 vs. ExPASy TrEMBL
Match: A0A2P5DLU8 (SAM-dependent methyltransferase SMT-type OS=Parasponia andersonii OX=3476 GN=PanWU01x14_050770 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.8e-133
Identity = 252/339 (74.34%), Postives = 261/339 (76.99%), Query Frame = 0 

Query: 21  MSKAGALDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCY 80
           MSKAGALDLASGLGGKI K EVLSAVEK                        YEKYHVCY
Sbjct: 1   MSKAGALDLASGLGGKIGKDEVLSAVEK------------------------YEKYHVCY 60

Query: 81  GGEEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNL 140
           GGEEEERKANY+DMVNKYYDLVTSFYEFGWGESFHFAP                      
Sbjct: 61  GGEEEERKANYSDMVNKYYDLVTSFYEFGWGESFHFAP---------------------- 120

Query: 141 NVHILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVT 200
                  RW GESLRESIKRHEHFLALQLG+KPGQKVLDVGCGIGGPLRE ARFSS SVT
Sbjct: 121 -------RWKGESLRESIKRHEHFLALQLGVKPGQKVLDVGCGIGGPLREFARFSSASVT 180

Query: 201 GLNNNEYQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKAEIEIGDGLPDIRLTGKCL 260
           GLNNNEYQISRGK            ELNR+A VD TC+FVKAEIEIGDGLPDIRLT KCL
Sbjct: 181 GLNNNEYQISRGK------------ELNRIAGVDNTCEFVKAEIEIGDGLPDIRLTTKCL 240

Query: 261 EALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRL 320
           EALKQAGFEV+WEKDLA +SP+PW+LPLDKSHFSLSSFRLTA+GRF TKNMVKALEF  L
Sbjct: 241 EALKQAGFEVIWEKDLAADSPIPWFLPLDKSHFSLSSFRLTAVGRFFTKNMVKALEFAGL 274

Query: 321 APKGSQRVQDFLEKAAEGLVAGGKKEIFTPMYFFLARKP 360
           APKGSQRVQDFLEKAAEGLV GGKKEIFTPMYFFLARKP
Sbjct: 301 APKGSQRVQDFLEKAAEGLVEGGKKEIFTPMYFFLARKP 274

[Close]
BLAST of Spg038852 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-116
Identity = 229/400 (57.25%), Postives = 254/400 (63.50%), Query Frame = 0 

Query: 27  LDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCYGGEEEE 86
           +DLAS LGGKIDK +VL+AVEK                        YE+YHV +GG EEE
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEK------------------------YEQYHVFHGGNEEE 60

Query: 87  RKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNVHILH 146
           RKANYTDMVNKYYDL TSFYE+GWGESFHFA                             
Sbjct: 61  RKANYTDMVNKYYDLATSFYEYGWGESFHFA----------------------------- 120

Query: 147 SRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNE 206
            RW GESLRESIKRHEHFLALQLG++PGQKVLDVGCGIGGPLREIARFS++ VTGLNNNE
Sbjct: 121 QRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNNE 180

Query: 207 YQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKA------------------------ 266
           YQI+RGK            ELNR+A VD+TC+FVKA                        
Sbjct: 181 YQITRGK------------ELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHA 240

Query: 267 -------------------------------------------EIEIGDGLPDIRLTGKC 326
                                                      EIEIGDGLPDIRLT KC
Sbjct: 241 PDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKC 300

Query: 327 LEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIR 360
           LEALKQAGFEV+WEKDLA +SP+PWYLPLDK+HFSLSSFRLTA+GRFITKNMVK LE+IR
Sbjct: 301 LEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIR 335

[Close]
BLAST of Spg038852 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-116
Identity = 229/400 (57.25%), Postives = 254/400 (63.50%), Query Frame = 0 

Query: 27  LDLASGLGGKIDKGEVLSAVEKYVDALNETGHSRIVYHVVCNDRISYEKYHVCYGGEEEE 86
           +DLAS LGGKIDK +VL+AVEK                        YE+YHV +GG EEE
Sbjct: 1   MDLASNLGGKIDKSDVLTAVEK------------------------YEQYHVFHGGNEEE 60

Query: 87  RKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNVHILH 146
           RKANYTDMVNKYYDL TSFYE+GWGESFHFA                             
Sbjct: 61  RKANYTDMVNKYYDLATSFYEYGWGESFHFA----------------------------- 120

Query: 147 SRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNE 206
            RW GESLRESIKRHEHFLALQLG++PGQKVLDVGCGIGGPLREIARFS++ VTGLNNNE
Sbjct: 121 QRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNNE 180

Query: 207 YQISRGKLDNCIFRFLLRQELNRVAKVDRTCDFVKA------------------------ 266
           YQI+RGK            ELNR+A VD+TC+FVKA                        
Sbjct: 181 YQITRGK------------ELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHA 240

Query: 267 -------------------------------------------EIEIGDGLPDIRLTGKC 326
                                                      EIEIGDGLPDIRLT KC
Sbjct: 241 PDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKC 300

Query: 327 LEALKQAGFEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIR 360
           LEALKQAGFEV+WEKDLA +SP+PWYLPLDK+HFSLSSFRLTA+GRFITKNMVK LE+IR
Sbjct: 301 LEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIR 335

[Close]
BLAST of Spg038852 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 142.9 bits (359), Expect = 5.1e-34
Identity = 102/337 (30.27%), Postives = 146/337 (43.32%), Query Frame = 0 

Query: 83  EEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNV 142
           +E E      D V+ +Y+LVT  YE+GWG+SFHF+P                        
Sbjct: 62  KEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIP--------------------- 121

Query: 143 HILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGL 202
                   G+S +++ + HE      + +KPGQK+LDVGCG+GGP+R IA  S  +V G+
Sbjct: 122 --------GKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRANVVGI 181

Query: 203 NNNEYQISRGKLDN-----------CIFRFL--------------------------LRQ 262
             NEYQ++R +L N               FL                          +  
Sbjct: 182 TINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYA 241

Query: 263 ELNRVAKVDR--------TCDFVKAE----------IEIGDGLPDIRLTGKCLEALKQAG 322
           E+ RV K           T +  KAE          IE GD LP +R      E  K+ G
Sbjct: 242 EIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVG 301

Query: 323 FEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRLAPKGSQR 365
           FE++ EKDLA     PW+  L          ++  +  +    +V+ L  + +APKG+  
Sbjct: 302 FEIVKEKDLASPPAEPWWTRL----------KMGRLAYWRNHIVVQILSAVGVAPKGTVD 359

[Close]
BLAST of Spg038852 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 139.4 bits (350), Expect = 5.6e-33
Identity = 101/337 (29.97%), Postives = 139/337 (41.25%), Query Frame = 0 

Query: 83  EEEERKANYTDMVNKYYDLVTSFYEFGWGESFHFAPRYDAISLLQLTDILYVSVFSNLNV 142
           +E E      D V+ +Y+LVT  YE+GWG+SFHF+P                        
Sbjct: 62  KEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSP------------------------ 121

Query: 143 HILHSRWNGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGL 202
           H+      G+S +++ + HE      + +KPGQK+LD GCG+GGP+R IA  S   VTG+
Sbjct: 122 HV-----PGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGI 181

Query: 203 NNNEYQISRGKLDN-----------CIFRFLL----RQELNRVAKVDRTCDFVKAE---- 262
             NEYQ+ R KL N               FL         +    ++ TC   K E    
Sbjct: 182 TINEYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYS 241

Query: 263 ------------------------------------IEIGDGLPDIRLTGKCLEALKQAG 322
                                               IE GD LP +R         K+ G
Sbjct: 242 EIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKVG 301

Query: 323 FEVLWEKDLAVNSPLPWYLPLDKSHFSLSSFRLTAIGRFITKNMVKALEFIRLAPKGSQR 365
           FEV+ EKDLA     PW+          +  ++  I  +    +V  L  I +APKG+  
Sbjct: 302 FEVVKEKDLAKPPSKPWW----------NRLKMGRIAYWRNHVVVVILSAIGVAPKGTVD 359

The following BLAST results are available for this feature:
BLAST of Spg038852 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6606886.14.8e-13565.23Cycloartenol-C-24-methyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG6581566.18.2e-13564.76Cycloartenol-C-24-methyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_023526267.18.2e-13564.76cycloartenol-C-24-methyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo]... [more]
XP_022935654.18.2e-13564.76cycloartenol-C-24-methyltransferase-like isoform X1 [Cucurbita moschata][more]
KHG15107.11.4e-13472.97Cycloartenol-C-24-methyltransferase -like protein [Gossypium arboreum][more]
BLAST of Spg038852 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LM022.6e-11557.25Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=SMT1 PE=1... [more]
Q6ZIX21.2e-10954.03Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q54I985.4e-5737.60Probable cycloartenol-C-24-methyltransferase 1 OS=Dictyostelium discoideum OX=44... [more]
Q6C2D92.7e-4835.00Sterol 24-C-methyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O... [more]
Q759S71.0e-4735.69Sterol 24-C-methyltransferase OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51... [more]
BLAST of Spg038852 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LD121.2e-13976.74SAM_MT_ERG6_SMT domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G68... [more]
A0A6J1F6164.0e-13564.76Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442447 PE=3 SV=1[more]
A0A0B0NR216.7e-13572.97Cycloartenol-C-24-methyltransferase-like protein OS=Gossypium arboreum OX=29729 ... [more]
A0A6J1DGP71.7e-13365.45Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020326 PE=3 SV=1[more]
A0A2P5DLU82.8e-13374.34SAM-dependent methyltransferase SMT-type OS=Parasponia andersonii OX=3476 GN=Pan... [more]
BLAST of Spg038852 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G13710.11.8e-11657.25sterol methyltransferase 1 [more]
AT5G13710.21.8e-11657.25sterol methyltransferase 1 [more]
AT1G20330.15.1e-3430.27sterol methyltransferase 2 [more]
AT1G76090.15.6e-3329.97sterol methyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 80..215
e-value: 1.1E-20
score: 75.9
NoneNo IPR availablePFAMPF02353CMAScoord: 163..216
e-value: 5.5E-5
score: 22.6
NoneNo IPR availablePANTHERPTHR44068:SF3METHYLTRANSFERASEcoord: 21..125
NoneNo IPR availablePANTHERPTHR44068:SF3METHYLTRANSFERASEcoord: 240..361
coord: 146..243
NoneNo IPR availablePANTHERPTHR44068ZGC:194242coord: 21..125
NoneNo IPR availablePANTHERPTHR44068ZGC:194242coord: 240..361
coord: 146..243
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 176..267
e-value: 5.17471E-4
score: 37.0243
IPR013705Sterol methyltransferase C-terminalPFAMPF08498Sterol_MT_Ccoord: 296..359
e-value: 7.8E-31
score: 106.0
IPR030384SAM-dependent methyltransferase SMT-typePROSITEPS51685SAM_MT_ERG6_SMTcoord: 98..359
score: 41.71146
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 97..360

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038852.1Spg038852.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0016020 membrane
molecular_function GO:0008168 methyltransferase activity