Spg038820 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg038820 vs. NCBI nr
Match: XP_038905301.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 601.7 bits (1550), Expect = 4.3e-168
Identity = 298/369 (80.76%), Postives = 320/369 (86.72%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEEIQNF  VWISIWICL YCYGVG +IP GITRF+FIFPVI LF YLPL+L SLHLGG
Sbjct: 1   MEEEIQNFLKVWISIWICLSYCYGVGERIPHGITRFLFIFPVISLFLYLPLILRSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSE------ 120
           LTAFF+AWLANFKLLLFAAG+GPLAA  M IGTFCAVASFPVK KQNP Q++SE      
Sbjct: 61  LTAFFIAWLANFKLLLFAAGQGPLAAPGMSIGTFCAVASFPVKLKQNPFQKTSEAAINGG 120

Query: 121 TATIIHPKSNQN-HHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLEL 180
           +A I    SN N H +SLLNY VKILLVAALVKAYDY+H+LPQKLLWL LCFHIYFLLEL
Sbjct: 121 SARINDTNSNHNRHRQSLLNYTVKILLVAALVKAYDYTHILPQKLLWLFLCFHIYFLLEL 180

Query: 181 SLAAAAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAK 240
           SLAAAA+AVA GG VELLPLFH+PYLSTSLQDFWGRRWNLVVTGILRPAV+RPAA AA K
Sbjct: 181 SLAAAASAVAFGGTVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATK 240

Query: 241 LLGRRWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKR 300
           LLG RW+P+AGV ATFLVSA MHELMFFYMSRQWPPPTWEV AFFV HGGCL  EM VKR
Sbjct: 241 LLGSRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVMAFFVFHGGCLVAEMAVKR 300

Query: 301 VWIDKWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNN 360
           +WID W+M P+ A VSVP TVGFFFWTCFWLFFPPF+NTCKADVRVVEEYAIFGAFVKNN
Sbjct: 301 LWIDTWQMRPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360

Query: 361 IPTGFFLLF 363
           IP+ F+  F
Sbjct: 361 IPSSFWSNF 369

BLAST of Spg038820 vs. NCBI nr
Match: XP_023523549.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593.6 bits (1529), Expect = 1.2e-165
Identity = 290/366 (79.23%), Postives = 315/366 (86.07%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           ME+EI     VWISIWICL YCYGVGNKI +G TRF+FIFPVIC+F YLPL LHSLHLGG
Sbjct: 1   MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSE------ 120
           LTAFFVAWLANFKLLLFAAG+GPLA   M I  FCAVASFP+K KQ P Q+SSE      
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGG 120

Query: 121 TATIIHPKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELS 180
            AT+I PKSNQNH ES  NYAVKILLVAALVKAYDY+H+LPQKL+W LLCFHIYF+L+LS
Sbjct: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180

Query: 181 LAAAAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKL 240
            AAA+AAV+ GG +ELLPLFH PYLSTSLQDFWGRRWNL VTGILRP  YRPA   A KL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 241 LGRRWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRV 300
           +G RW+P+AGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMV KR+
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 301 WIDKWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNN- 360
           WID W+MPP+ A VSVP TVGFFFWTCFWLFFPPF+NTCKADVRVV+EY IFGAF+KNN 
Sbjct: 301 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNI 360

BLAST of Spg038820 vs. NCBI nr
Match: XP_022998457.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 577.0 bits (1486), Expect = 1.1e-160
Identity = 281/358 (78.49%), Postives = 307/358 (85.75%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           ME+EI N   VWISIWICL YCYGVG KI +G TRF+ IFPVIC+F YLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSE------ 120
           LTAFFVAWLANFKLLLFAAG+GPLA   M I  FCAVASFP+K KQ   Q+SSE      
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 121 TATIIHPKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELS 180
            AT+I PKSNQNH ES  NYAVKILLVAALVKAYDY+H+L QKL+W LLCFHIYF+L+LS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 181 LAAAAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKL 240
            AAA+AAV+ GG +ELLPLFH PYLSTSLQDFWGRRWNL VTGILRP  YRPA   A KL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 241 LGRRWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRV 300
           +G RW+P+AGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMV KR+
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 301 WIDKWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKN 353
           WID W+MPP+ A+VSVP TVGFFFWTCFWLFFPPF+NTCKADVRVV+EY IFGAF+KN
Sbjct: 301 WIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 358

BLAST of Spg038820 vs. NCBI nr
Match: XP_004149731.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58562.1 hypothetical protein Csa_002341 [Cucumis sativus])

HSP 1 Score: 573.2 bits (1476), Expect = 1.6e-159
Identity = 281/360 (78.06%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           ME+EI NF  VWISIWICL YCYGVG +IP GITRF+FIFPVI LF YLPLLLHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
           LTAFF+AWLANFKLLLF+AG GPLAA  + IGTFCA+ASFPVK KQ PLQ++SE A  IH
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFA--IH 120

Query: 121 ----PKSNQN-HHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSL 180
                KS+ N H +S L+YAVKI LVA +VKAY+Y+H+LPQKL WL LCFHIYFLLELSL
Sbjct: 121 GGLPSKSDHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 AAAAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLL 240
           AAAA+ VACGG +ELLPLFH+PY STSLQDFWGRRWNLVVTGILRPAV+RPAA AA+KLL
Sbjct: 181 AAAASIVACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASKLL 240

Query: 241 GRRWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVW 300
           G++  P+AGV ATFLVSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EM VKRVW
Sbjct: 241 GKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKRVW 300

Query: 301 IDKWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNNIP 356
           I +WRM P+  IVSVP TVGFFFW+CFWLFFPPF+N+CKADVRVVEEYA+FGAF+KNNIP
Sbjct: 301 IQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNNIP 358

BLAST of Spg038820 vs. NCBI nr
Match: XP_008461897.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo])

HSP 1 Score: 572.8 bits (1475), Expect = 2.1e-159
Identity = 280/366 (76.50%), Postives = 312/366 (85.25%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEEI NF  VWIS WICL YCYGVG +IP GITRF+FI PV+ LF YLPLLLHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETAT--I 120
           LTAFF+AWLANFKLLLF+AG GPL+A  + +GTFCA+ASFPVK KQNPLQ++SE A    
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 168

Query: 121 IHPKSNQNHH-ESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAA 180
           +  KS+ N H +S L+YAVKI LVAA+VKAY+Y+H+LPQKL WL LCFHIYFLLELSLAA
Sbjct: 169 LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSLAA 228

Query: 181 AAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGR 240
           AA+ VA GG VELLPLFH PY STSLQDFWGRRWNLVVTGILRPAV+ PAA AA+KL G 
Sbjct: 229 AASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASKLFGS 288

Query: 241 RWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWID 300
           RW PI GV ATF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMVVKRVWI 
Sbjct: 289 RWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKRVWIQ 348

Query: 301 KWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNNIPTG 360
           KW M P+ +IV+VP TVGFFFW+CFWLFFPPF+N+CKADVRVVEEYA+FGAFVKNNIP+ 
Sbjct: 349 KWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNNIPSS 408

Query: 361 FFLLFK 364
            F  FK
Sbjct: 409 LFPNFK 414

BLAST of Spg038820 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.9e-73
Identity = 154/345 (44.64%), Postives = 212/345 (61.45%), Query Frame = 0

Query: 7   NFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGGLTAFFV 66
           +F   W  + I LCY + +   +P+GI R +  FPV  +FF +P L++SLHL G+TAFF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGRGPLAA--APMCIGTFCAVASFPVKFKQNPLQESSETATIIHPKSN 126
           AWLANFKLLLFA GRGPL++   P+ +  F AV+  P+K + +P    +         S+
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTH--------SH 122

Query: 127 QNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAAAVAC 186
           +   E  L Y +K + V  ++KAY+YS  LP+K++  L   HIYF LE+ LAA AAAV  
Sbjct: 123 EGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRA 182

Query: 187 GGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWSPIAG 246
              +EL P F++PYL+TSLQDFWGRRWNL+VTGILRP VY P+    + +LG  +S I  
Sbjct: 183 MSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILA 242

Query: 247 VAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWIDKWRMPPV 306
              TF+VS  MHEL+FFYM R    P W++  FF+++G C  VE+ +K+    +WR P  
Sbjct: 243 AFGTFVVSGIMHELIFFYMGRL--RPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKA 302

Query: 307 SAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAF 350
              +S   T+ F   T  WLF P F N C    + ++EYA  GAF
Sbjct: 303 ---ISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

BLAST of Spg038820 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.1e-61
Identity = 143/353 (40.51%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           M+EE++N   VW+S  I + YCY +  +I  G  RF+ + PV+ LF  LPL   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
           +TAFF+ WLANFKL+LF+  +GPL   P     F     FP+K +QNP            
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQNP------------ 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
              +QNH   L+ +A+K+ + A L+  Y Y   L   +L  L   H+Y  +E+ L     
Sbjct: 121 --KSQNHLPKLV-FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKV 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            V      +L P  ++PYL+TSLQDFWGRRWNL+V  ILRPAVY P    + + +   W+
Sbjct: 181 VVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKR--VWIDK 300
              G+ A F+VS  +HEL+FFY+ R+   PT EV  FFVLHG C AVE+ VK+      +
Sbjct: 241 LFPGILAAFIVSGLVHELLFFYLIREM--PTGEVTLFFVLHGVCTAVELAVKKKTTVAQR 300

Query: 301 WRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVK 352
           WR+ P    VS   TVGF F T  WLF P    +   + R   E  +   F+K
Sbjct: 301 WRLSPG---VSRVLTVGFVFVTGGWLFTPQLKRSGVME-RFTSEAVLLVEFIK 332

BLAST of Spg038820 vs. ExPASy Swiss-Prot
Match: Q4PT07 (Probable long-chain-alcohol O-fatty-acyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=At1g34500 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.7e-61
Identity = 139/352 (39.49%), Postives = 197/352 (55.97%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEE++NF IVWIS  I + YCY +   I  G+ R   + P+  LFF LPL   S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
            TAF+++ +A+ KL+LFA  +GPL      +  F     FP+K ++NP  + S       
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAPNLIQFVCFTCFPIKLQRNPKSQPS------- 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
               QNH      +A+KI++   ++  Y+YSH LPQ +L  L   H+Y  LE+ L     
Sbjct: 121 ----QNHFHKRA-FAIKIMIFGVVLHVYNYSHFLPQTVLLSLCFLHLYVELEILLGPLKV 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            ++     +L P F++PYL+TSLQDFWGRRWNL+V+ +LR  +Y P   A  + +   W+
Sbjct: 181 LLSMALGCDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQRPMNSGWA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKR-VWIDKW 300
              G   TFLVS   HEL++FY++R+   PTWEV  FFVL+G C   E+ VKR  ++ +W
Sbjct: 241 RFMGYLVTFLVSGLFHELVYFYITRE--TPTWEVTLFFVLNGVCTGTEVAVKRTAFLQRW 300

Query: 301 RMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVK 352
              PV + VS   T+GF   T   LFFP F+ +   + R   E   F  FVK
Sbjct: 301 W--PVRSSVSRLLTMGFVVVTGGLLFFPLFIRSGMME-RRANETLFFLDFVK 335

BLAST of Spg038820 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.2e-61
Identity = 143/353 (40.51%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEE+ +   VW+   I + YCY    +I  G+ R + + PV  LF  LPL + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
            TAFF++WLANFKL+LF+  +GPL   P  +  F     FP+K +QNP  ++        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNPKPQNQ------M 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
           PK           +AVK+     L+  Y+Y   +   +L +L   HIY   E+ LA    
Sbjct: 121 PK---------WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKV 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            ++     +L P F+ PYLSTSLQDFWGRRWNL+V  ILRPAVY P    A + +    +
Sbjct: 181 LLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRV-WIDKW 300
              GV A+FLVS  +HEL+FFY +R+   PT EV  FFVLHG C A E   KR   + +W
Sbjct: 241 LFLGVFASFLVSGVVHELIFFYFTRE--SPTGEVTLFFVLHGVCTAAECAAKRTRLVRRW 300

Query: 301 RMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKN 353
           +   VS +VS   TVGF   T  WLFFP    +   + R+ +E  +F  FVK+
Sbjct: 301 K---VSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

BLAST of Spg038820 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.9e-60
Identity = 135/355 (38.03%), Postives = 197/355 (55.49%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQ-GITRFVFIFPVICLFFYLPLLLHSLHLG 60
           +E+E++ F  VWIS     CYC  V    P  G  R + + PV+ LF +LPL L S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GLTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATII 120
           G TA ++ WLANFKLLLFA   GPL+   + +  F +    P+KF+ +P  +  +     
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLSNPSLSLLHFISTTLLPIKFRDDPSNDHEK----- 122

Query: 121 HPKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAA 180
           + ++       ++ +  K++  A ++K Y++   LP  ++ +L CFH Y   E++LAA+A
Sbjct: 123 NKRTLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASA 182

Query: 181 AAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRW 240
                   ++L P F+ PYL+TSLQDFWGRRWNL+V+ IL    Y+P     ++ +  RW
Sbjct: 183 VIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRW 242

Query: 241 SPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWIDKW 300
                +   F VS  MHE+ FFY++R    P+WEV  FFVLHG C AVEMVVK+    K 
Sbjct: 243 EVAGAMLVAFTVSGLMHEVFFFYLTR--ARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKV 302

Query: 301 RMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNNI 355
           R+      VS   TVGF   T  WLF P  V     D++ ++EY +   + +  +
Sbjct: 303 RL---RREVSGALTVGFVMVTGGWLFLPQLVRH-GVDLKTIDEYPVMFNYTQKKL 346

BLAST of Spg038820 vs. ExPASy TrEMBL
Match: A0A6J1K824 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493084 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.5e-161
Identity = 281/358 (78.49%), Postives = 307/358 (85.75%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           ME+EI N   VWISIWICL YCYGVG KI +G TRF+ IFPVIC+F YLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSE------ 120
           LTAFFVAWLANFKLLLFAAG+GPLA   M I  FCAVASFP+K KQ   Q+SSE      
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 121 TATIIHPKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELS 180
            AT+I PKSNQNH ES  NYAVKILLVAALVKAYDY+H+L QKL+W LLCFHIYF+L+LS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 181 LAAAAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKL 240
            AAA+AAV+ GG +ELLPLFH PYLSTSLQDFWGRRWNL VTGILRP  YRPA   A KL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 241 LGRRWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRV 300
           +G RW+P+AGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMV KR+
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 301 WIDKWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKN 353
           WID W+MPP+ A+VSVP TVGFFFWTCFWLFFPPF+NTCKADVRVV+EY IFGAF+KN
Sbjct: 301 WIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 358

BLAST of Spg038820 vs. ExPASy TrEMBL
Match: A0A0A0LD00 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684680 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 7.9e-160
Identity = 281/360 (78.06%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           ME+EI NF  VWISIWICL YCYGVG +IP GITRF+FIFPVI LF YLPLLLHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
           LTAFF+AWLANFKLLLF+AG GPLAA  + IGTFCA+ASFPVK KQ PLQ++SE A  IH
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFA--IH 120

Query: 121 ----PKSNQN-HHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSL 180
                KS+ N H +S L+YAVKI LVA +VKAY+Y+H+LPQKL WL LCFHIYFLLELSL
Sbjct: 121 GGLPSKSDHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 AAAAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLL 240
           AAAA+ VACGG +ELLPLFH+PY STSLQDFWGRRWNLVVTGILRPAV+RPAA AA+KLL
Sbjct: 181 AAAASIVACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASKLL 240

Query: 241 GRRWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVW 300
           G++  P+AGV ATFLVSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EM VKRVW
Sbjct: 241 GKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKRVW 300

Query: 301 IDKWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNNIP 356
           I +WRM P+  IVSVP TVGFFFW+CFWLFFPPF+N+CKADVRVVEEYA+FGAF+KNNIP
Sbjct: 301 IQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNNIP 358

BLAST of Spg038820 vs. ExPASy TrEMBL
Match: A0A1S3CFN5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500392 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.0e-159
Identity = 280/366 (76.50%), Postives = 312/366 (85.25%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEEI NF  VWIS WICL YCYGVG +IP GITRF+FI PV+ LF YLPLLLHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETAT--I 120
           LTAFF+AWLANFKLLLF+AG GPL+A  + +GTFCA+ASFPVK KQNPLQ++SE A    
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 168

Query: 121 IHPKSNQNHH-ESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAA 180
           +  KS+ N H +S L+YAVKI LVAA+VKAY+Y+H+LPQKL WL LCFHIYFLLELSLAA
Sbjct: 169 LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSLAA 228

Query: 181 AAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGR 240
           AA+ VA GG VELLPLFH PY STSLQDFWGRRWNLVVTGILRPAV+ PAA AA+KL G 
Sbjct: 229 AASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASKLFGS 288

Query: 241 RWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWID 300
           RW PI GV ATF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMVVKRVWI 
Sbjct: 289 RWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKRVWIQ 348

Query: 301 KWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNNIPTG 360
           KW M P+ +IV+VP TVGFFFW+CFWLFFPPF+N+CKADVRVVEEYA+FGAFVKNNIP+ 
Sbjct: 349 KWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNNIPSS 408

Query: 361 FFLLFK 364
            F  FK
Sbjct: 409 LFPNFK 414

BLAST of Spg038820 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 5.0e-154
Identity = 273/363 (75.21%), Postives = 305/363 (84.02%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEEI NF  VWIS WICL YCYGVG +IP GITRF+FI PV+ LF YLPLLLHSLHLGG
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETAT--I 120
           LTAFF+AWLANFKLLLF+AG GPL+A  + +GTFCA+ASFPVK KQNPLQ++SE A    
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 120

Query: 121 IHPKSNQNHH-ESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAA 180
           +  KS+ N H +S L+YAVKI LVAA+VKAY+Y+H+LPQKL WL LCFHIYFLLELSLAA
Sbjct: 121 LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSLAA 180

Query: 181 AAAAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGR 240
           AA+ VA GG VELLPLFH PY STSLQDFWGRRWNLVVTGILRPAV+ PAA AA+KL G 
Sbjct: 181 AASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASKLFGS 240

Query: 241 RWSPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWID 300
           RW PI GV ATF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMVVKRVWI 
Sbjct: 241 RWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKRVWIQ 300

Query: 301 KWRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKNNIPTG 360
           KW M P+ +IV+VP TVGFFFW+CFWLFFPPF+N+CKADVRVVEEYA+FG F       G
Sbjct: 301 KWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF-------G 356

BLAST of Spg038820 vs. ExPASy TrEMBL
Match: A0A5N5FGI4 (Acyl-CoA--sterol O-acyltransferase 1 OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_001856 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 5.2e-95
Identity = 187/353 (52.97%), Postives = 239/353 (67.71%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           ME EI NF  VW+S+++ LCYCY +   +P+G+ R + I P++ LF YLPL L SLHLGG
Sbjct: 1   MEGEIGNFIKVWLSVYLSLCYCYAIAKFVPKGVPRLLLIIPIVSLFLYLPLHLSSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAP-MCIGTFCAVASFPVKFKQNPLQESSETATII 120
            TAFF++WLANFKLLLFA G+GPL + P + +  F AVA  P+K +QNP  ++       
Sbjct: 61  TTAFFISWLANFKLLLFAFGKGPLCSDPSISLLRFVAVACLPIKIQQNPYSKTQNKENPA 120

Query: 121 HPKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAA 180
            PK  +N  +S LNYAVK+LL A LVK YDY   +  KLLW++ CFHIYF LE+ L  AA
Sbjct: 121 -PKITKNGTKSPLNYAVKVLLFALLVKLYDYKEHIHPKLLWIMYCFHIYFALEIILVIAA 180

Query: 181 AAVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRW 240
                   +EL P F+ PYLS+SLQDFWGRRWN++VT ILRP VY P    A +++GRRW
Sbjct: 181 TLARALLGLELEPQFNEPYLSSSLQDFWGRRWNIMVTSILRPTVYEPILALATRVVGRRW 240

Query: 241 SPIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWIDKW 300
           + +AGV  TF+VSA MHEL+F+Y+ R    PTWE+  FF+LHG CL  E+ VK+  + K 
Sbjct: 241 ASLAGVMGTFVVSAIMHELIFYYLGR--TQPTWEITWFFLLHGACLMAEIGVKKA-VGKR 300

Query: 301 RMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKN 353
           +   +   VS P TV F   T FWLFFPP +  CKADVR +EEYA  GAFVK+
Sbjct: 301 KKLQLPRSVSGPLTVAFVTATVFWLFFPPLLR-CKADVRALEEYAAVGAFVKH 348

BLAST of Spg038820 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 277.7 bits (709), Expect = 1.3e-74
Identity = 154/345 (44.64%), Postives = 212/345 (61.45%), Query Frame = 0

Query: 7   NFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGGLTAFFV 66
           +F   W  + I LCY + +   +P+GI R +  FPV  +FF +P L++SLHL G+TAFF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGRGPLAA--APMCIGTFCAVASFPVKFKQNPLQESSETATIIHPKSN 126
           AWLANFKLLLFA GRGPL++   P+ +  F AV+  P+K + +P    +         S+
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTH--------SH 122

Query: 127 QNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAAAVAC 186
           +   E  L Y +K + V  ++KAY+YS  LP+K++  L   HIYF LE+ LAA AAAV  
Sbjct: 123 EGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRA 182

Query: 187 GGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWSPIAG 246
              +EL P F++PYL+TSLQDFWGRRWNL+VTGILRP VY P+    + +LG  +S I  
Sbjct: 183 MSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILA 242

Query: 247 VAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWIDKWRMPPV 306
              TF+VS  MHEL+FFYM R    P W++  FF+++G C  VE+ +K+    +WR P  
Sbjct: 243 AFGTFVVSGIMHELIFFYMGRL--RPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKA 302

Query: 307 SAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAF 350
              +S   T+ F   T  WLF P F N C    + ++EYA  GAF
Sbjct: 303 ---ISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

BLAST of Spg038820 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.5e-62
Identity = 143/353 (40.51%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           M+EE++N   VW+S  I + YCY +  +I  G  RF+ + PV+ LF  LPL   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
           +TAFF+ WLANFKL+LF+  +GPL   P     F     FP+K +QNP            
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQNP------------ 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
              +QNH   L+ +A+K+ + A L+  Y Y   L   +L  L   H+Y  +E+ L     
Sbjct: 121 --KSQNHLPKLV-FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKV 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            V      +L P  ++PYL+TSLQDFWGRRWNL+V  ILRPAVY P    + + +   W+
Sbjct: 181 VVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKR--VWIDK 300
              G+ A F+VS  +HEL+FFY+ R+   PT EV  FFVLHG C AVE+ VK+      +
Sbjct: 241 LFPGILAAFIVSGLVHELLFFYLIREM--PTGEVTLFFVLHGVCTAVELAVKKKTTVAQR 300

Query: 301 WRMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVK 352
           WR+ P    VS   TVGF F T  WLF P    +   + R   E  +   F+K
Sbjct: 301 WRLSPG---VSRVLTVGFVFVTGGWLFTPQLKRSGVME-RFTSEAVLLVEFIK 332

BLAST of Spg038820 vs. TAIR 10
Match: AT1G34500.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.4e-62
Identity = 139/352 (39.49%), Postives = 197/352 (55.97%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEE++NF IVWIS  I + YCY +   I  G+ R   + P+  LFF LPL   S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
            TAF+++ +A+ KL+LFA  +GPL      +  F     FP+K ++NP  + S       
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAPNLIQFVCFTCFPIKLQRNPKSQPS------- 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
               QNH      +A+KI++   ++  Y+YSH LPQ +L  L   H+Y  LE+ L     
Sbjct: 121 ----QNHFHKRA-FAIKIMIFGVVLHVYNYSHFLPQTVLLSLCFLHLYVELEILLGPLKV 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            ++     +L P F++PYL+TSLQDFWGRRWNL+V+ +LR  +Y P   A  + +   W+
Sbjct: 181 LLSMALGCDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQRPMNSGWA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKR-VWIDKW 300
              G   TFLVS   HEL++FY++R+   PTWEV  FFVL+G C   E+ VKR  ++ +W
Sbjct: 241 RFMGYLVTFLVSGLFHELVYFYITRE--TPTWEVTLFFVLNGVCTGTEVAVKRTAFLQRW 300

Query: 301 RMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVK 352
              PV + VS   T+GF   T   LFFP F+ +   + R   E   F  FVK
Sbjct: 301 W--PVRSSVSRLLTMGFVVVTGGLLFFPLFIRSGMME-RRANETLFFLDFVK 335

BLAST of Spg038820 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.4e-62
Identity = 143/353 (40.51%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEEE+ +   VW+   I + YCY    +I  G+ R + + PV  LF  LPL + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
            TAFF++WLANFKL+LF+  +GPL   P  +  F     FP+K +QNP  ++        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNPKPQNQ------M 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
           PK           +AVK+     L+  Y+Y   +   +L +L   HIY   E+ LA    
Sbjct: 121 PK---------WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKV 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            ++     +L P F+ PYLSTSLQDFWGRRWNL+V  ILRPAVY P    A + +    +
Sbjct: 181 LLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRV-WIDKW 300
              GV A+FLVS  +HEL+FFY +R+   PT EV  FFVLHG C A E   KR   + +W
Sbjct: 241 LFLGVFASFLVSGVVHELIFFYFTRE--SPTGEVTLFFVLHGVCTAAECAAKRTRLVRRW 300

Query: 301 RMPPVSAIVSVPATVGFFFWTCFWLFFPPFVNTCKADVRVVEEYAIFGAFVKN 353
           +   VS +VS   TVGF   T  WLFFP    +   + R+ +E  +F  FVK+
Sbjct: 301 K---VSQMVSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

BLAST of Spg038820 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 229.6 bits (584), Expect = 4.1e-60
Identity = 138/325 (42.46%), Postives = 183/325 (56.31%), Query Frame = 0

Query: 1   MEEEIQNFFIVWISIWICLCYCYGVGNKIPQGITRFVFIFPVICLFFYLPLLLHSLHLGG 60
           MEE+ +N   VWIS  I L YCY + +K+ +G+ R + I PV  LF  LPL L  +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  LTAFFVAWLANFKLLLFAAGRGPLAAAPMCIGTFCAVASFPVKFKQNPLQESSETATIIH 120
           ++  F++WLANFKLLLFA   GPL   P  +  F   A  P+K +Q+P   S      +H
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPPKLSRFICFACLPIKIRQDP---SPNAIPNLH 120

Query: 121 PKSNQNHHESLLNYAVKILLVAALVKAYDYSHLLPQKLLWLLLCFHIYFLLELSLAAAAA 180
           PK            AVKIL++  L+  Y+Y   LP+ ++  L C HIY  +EL L    A
Sbjct: 121 PKPMPK-----WVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGA 180

Query: 181 AVACGGAVELLPLFHRPYLSTSLQDFWGRRWNLVVTGILRPAVYRPAAYAAAKLLGRRWS 240
            V+      + P+F+ PYL+TSLQDFW RRWNL+V+ +LR  V+ P      ++L    +
Sbjct: 181 VVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGA 240

Query: 241 PIAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVVKRVWIDKWR 300
             AGV A+F VS  MHEL++FYM R+  PPTWEV  FFVLHG   A E+ VKR    +W 
Sbjct: 241 MFAGVMASFFVSGLMHELLYFYMIRK--PPTWEVTCFFVLHGAATATEIAVKRT---QWL 300

Query: 301 MPPVSAIVSVPATVGFFFWTCFWLF 326
            PP  A VS    + F   T  WLF
Sbjct: 301 RPPHRA-VSGLVVLTFVSVTGVWLF 311